Issue Information
FEMS Microbiology Letters,Volume 361, Issue 2, Page i-ii, December 2014. (Source: FEMS Microbiology Letters)
Source: FEMS Microbiology Letters - December 22, 2014 Category: Microbiology Source Type: research
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FEMS Microbiology Letters,Volume 361, Issue 2, Page i-ii, December 2014. (Source: FEMS Microbiology Letters)
Source: FEMS Microbiology Letters - December 22, 2014 Category: Microbiology Source Type: research
Issue Information
(Source: FEMS Microbiology Letters)
Source: FEMS Microbiology Letters - December 22, 2014 Category: Microbiology Tags: Issue Information Source Type: research
Issue Information
FEMS Microbiology Letters,Volume 361, Issue 1, Page i-ii, December 2014. (Source: FEMS Microbiology Letters)
Source: FEMS Microbiology Letters - December 8, 2014 Category: Microbiology Source Type: research
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FEMS Microbiology Letters,Volume 361, Issue 1, Page i-ii, December 2014. (Source: FEMS Microbiology Letters)
Source: FEMS Microbiology Letters - December 8, 2014 Category: Microbiology Source Type: research
Issue Information
(Source: FEMS Microbiology Letters)
Source: FEMS Microbiology Letters - December 8, 2014 Category: Microbiology Tags: Issue Information Source Type: research
The thymidylate kinase genes from Mycobacterium tuberculosis and methicillin‐resistant Staphylococcus aureus confer 3′‐azido‐3′‐deoxythymidine resistance to Escherichia coli
This study investigates the ability of an evolutionarily conserved essential gene to provide resistance against antimicrobial compounds. An Escherichia coli chromosomally encoded thymidylate kinase (tmk) conditional lethal strain was developed to investigate tmk alleles from relevant nosocomial pathogens. The thymidylate kinase conditional lethal strain harboring a plasmid with a tmk gene from Mycobacterium tuberculosis, methicillin‐resistant Staphylococcus aureus (MRSA), or Pseudomonas aeruginosa downstream of an inducible promoter was examined for survival against increasing concentrations of 3′‐azido‐3′‐deox...
Source: FEMS Microbiology Letters - November 5, 2014 Category: Microbiology Authors: Jay H. Russell, Marc Ostermeier Tags: Research Letter Source Type: research
Differential expression of the seven rRNA operon promoters from the plant growth ‐promoting bacterium Pseudomonas sp. UW4
Abstract
Bacteria often have multiple copies of ribosomal RNA (rrn) genes in their genomes. The presence of multiple rrn operons suggests an advantage to the organism, perhaps through adjustable control of protein expression in response to altered environmental conditions. In the work described here, the strengths of the seven rRNA promoters of Pseudomonas sp. UW4 were individually assessed by separately cloning each promoter region into an expression vector and monitoring the activity of the reporter protein, the Escherichia coli lacZ gene product. The lacZ expression was the highest for the rrnE promoter under all growth...
Source: FEMS Microbiology Letters - November 4, 2014 Category: Microbiology Authors: Jin Duan, Lori Reimer, John J. Heikkila, Bernard R. Glick Tags: Research Letter Source Type: research
The thymidylate kinase genes from Mycobacterium tuberculosis and methicillin ‐resistant Staphylococcus aureus confer 3′‐azido‐3′‐deoxythymidine resistance to Escherichia coli
This study investigates the ability of an evolutionarily conserved essential gene to provide resistance against antimicrobial compounds. An Escherichia coli chromosomally encoded thymidylate kinase (tmk) conditional lethal strain was developed to investigate tmk alleles from relevant nosocomial pathogens. The thymidylate kinase conditional lethal strain harboring a plasmid with a tmk gene from Mycobacterium tuberculosis, methicillin‐resistant Staphylococcus aureus (MRSA), or Pseudomonas aeruginosa downstream of an inducible promoter was examined for survival against increasing concentrations of 3′‐azido‐3′‐deox...
Source: FEMS Microbiology Letters - November 4, 2014 Category: Microbiology Authors: Jay H. Russell, Marc Ostermeier Tags: Research Letter Source Type: research
Antibacterial effects of protruding and recessed shark skin micropatterned surfaces of polyacrylate plate with a shallow groove
Abstract
Antibacterial effects in terms of biofilm formation and swarming motility were studied using polyacrylate plates having protruding or recessed shark skin micropatterned surfaces with a shallow groove (2 μm pattern width and spacing, 0.4 μm pattern height). It was found that biofilm formation and swarming motility of Pseudomonas aeruginosa were strongly inhibited by the shark skin pattern plates with a shallow (0.4 μm) pattern height. Biofilm formation of Staphylococcus aureus was also strongly inhibited. Live bacteria were located on the pattern rather than in the spacing. When the shape of pattern was a lin...
Source: FEMS Microbiology Letters - October 31, 2014 Category: Microbiology Authors: Akihiko Sakamoto, Yusuke Terui, Chihiro Horie, Takashi Fukui, Toshiyuki Masuzawa, Shintaro Sugawara, Kaku Shigeta, Tatsuo Shigeta, Kazuei Igarashi, Keiko Kashiwagi Tags: Research Letter Source Type: research
Selenate reductase activity in Escherichia coli requires Isc iron–sulfur cluster biosynthesis genes
This study investigates the genes involved in microbial selenium reduction. (Source: FEMS Microbiology Letters)
Source: FEMS Microbiology Letters - October 31, 2014 Category: Microbiology Authors: Nathan Yee, Jessica Choi, Abigail W. Porter, Sean Carey, Ines Rauschenbach, Arye Harel Tags: Research Letter Source Type: research
Gp66, a calcineurin family phosphatase encoded by mycobacteriophage D29, is a 2′, 3′ cyclic nucleotide phosphodiesterase that negatively regulates phage growth
Abstract
Mycobacteriophage D29 encodes a protein Gp66 which has been predicted to be a calcineurin family phosphoesterase. Phylogenetically Gp66 and related proteins mostly derived from mycobacteriophages form a distinct clade within this family. Interestingly, the presence of gene 66 orthologs can be traced to bacteria of diverse phylogenetic lineages such as Aquifex aeolicus, a deep branching eubacteria and Methanococcus jannaschii, an archaebacteria. The promiscuous nature of gene 66 suggests that it may have been transferred across genus barriers by horizontal gene transfer mechanisms. The biological function of member...
Source: FEMS Microbiology Letters - October 31, 2014 Category: Microbiology Authors: Soumita Dutta, Niketa Bhawsinghka, Sujoy K. Das Gupta Tags: Research Letter Source Type: research
Efficiency of rep ‐PCR fingerprinting as a useful technique for molecular typing of plant pathogenic fungal species: Botryosphaeriaceae species as a case study
In this study, we used rep‐PCR technique as a molecular tool for typing and differentiation of the Botryosphaeriaceae species, well‐known and cosmopolitan fungal pathogens on woody plants. Three primer sets BOX, ERIC and REP were used to differentiate 27 species belong to eight genera. The majority of them were examined in terms of typing and differentiation using molecular methods for the first time. All the primer sets were able to generate species‐specific DNA fingerprints from all the tested strains, with two exceptions in the genera Diplodia and Spencermartinsia. Despite the deficiency of each primer sets to sep...
Source: FEMS Microbiology Letters - October 30, 2014 Category: Microbiology Authors: Jafar Abdollahzadeh, Sajedeh Zolfaghari Tags: Research Letter Source Type: research
