Issue Information
FEMS Yeast Research,Volume 14, Issue 8, Page i-ii, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - December 8, 2014 Category: Research Source Type: research

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FEMS Yeast Research,Volume 14, Issue 8, Page i-ii, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - December 8, 2014 Category: Research Source Type: research

Issue Information
(Source: FEMS Yeast Research)
Source: FEMS Yeast Research - December 8, 2014 Category: Research Tags: Issue Information Source Type: research

Serum albumin promotes ATP‐binding cassette transporter‐dependent sterol uptake in yeast
Abstract Sterol uptake in fungi is a multistep process that involves interaction between external sterols and the cell wall, incorporation of sterol molecules into the plasma membrane, and subsequent integration into intracellular membranes for turnover. ATP‐binding cassette (ABC) transporters have been implicated in sterol uptake, but key features of their activity remain to be elucidated. Here, we apply fluorescent cholesterol (NBD‐cholesterol) to monitor sterol uptake under anaerobic and aerobic conditions in two fungal species, Candida glabrata (Cg) and Saccharomyces cerevisiae (Sc). We found that in both fungal sp...
Source: FEMS Yeast Research - November 17, 2014 Category: Research Authors: Magdalena Marek, Daniele Silvestro, Maria D. Fredslund, Tonni G. Andersen, Thomas G. Pomorski Tags: Research Article Source Type: research

Serum albumin promotes ATP ‐binding cassette transporter‐dependent sterol uptake in yeast
Abstract Sterol uptake in fungi is a multistep process that involves interaction between external sterols and the cell wall, incorporation of sterol molecules into the plasma membrane, and subsequent integration into intracellular membranes for turnover. ATP‐binding cassette (ABC) transporters have been implicated in sterol uptake, but key features of their activity remain to be elucidated. Here, we apply fluorescent cholesterol (NBD‐cholesterol) to monitor sterol uptake under anaerobic and aerobic conditions in two fungal species, Candida glabrata (Cg) and Saccharomyces cerevisiae (Sc). We found that in both fungal sp...
Source: FEMS Yeast Research - November 16, 2014 Category: Research Authors: Magdalena Marek, Daniele Silvestro, Maria D. Fredslund, Tonni G. Andersen, Thomas G. Pomorski Tags: Research Article Source Type: research

Yeast tolerance to the ionic liquid 1 ‐ethyl‐3‐methylimidazolium acetate
Abstract Lignocellulosic plant biomass is the target feedstock for production of second‐generation biofuels. Ionic liquid (IL) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. Although biomass is typically washed following IL pretreatment, small quantities of residual IL can inhibit fermentative microorganisms downstream, such as the widely used ethanologenic yeast, Saccharomyces cerevisiae. The aim of this study was to identify yeasts tolerant to the IL 1‐ethyl‐3‐methylimidazolium acetate, one of the top performing ILs known for biomass pretreatment. ...
Source: FEMS Yeast Research - November 11, 2014 Category: Research Authors: Irnayuli R. Sitepu, Shuang Shi, Blake A. Simmons, Steven W. Singer, Kyria Boundy ‐Mills, Christopher W. Simmons Tags: Research Article Source Type: research

Issue Information
(Source: FEMS Yeast Research)
Source: FEMS Yeast Research - November 10, 2014 Category: Research Tags: Issue Information Source Type: research

Cadmium‐induced activation of high osmolarity glycerol pathway through its Sln1 branch is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in budding yeast
Abstract Cadmium ions disrupt reactive oxygen species/Ca2+ homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive to a sublethal concentration of cadmium, and 64 mutants show elevated intracellular cadmium concentrations upon exposure to cadmium. Hog1 is phosphorylated, reaching a peak 30 min after the cadmium treatment. Both Sln1 and Sho1 upstream branches are involved in the cadmium‐induced activation of high ...
Source: FEMS Yeast Research - November 10, 2014 Category: Research Authors: Linghuo Jiang, Chunlei Cao, Lilin Zhang, Wei Lin, Jing Xia, Huihui Xu, Yan Zhang Tags: Research Article Source Type: research

Cadmium ‐induced activation of high osmolarity glycerol pathway through its Sln1 branch is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in budding yeast
Abstract Cadmium ions disrupt reactive oxygen species/Ca2+ homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive to a sublethal concentration of cadmium, and 64 mutants show elevated intracellular cadmium concentrations upon exposure to cadmium. Hog1 is phosphorylated, reaching a peak 30 min after the cadmium treatment. Both Sln1 and Sho1 upstream branches are involved in the cadmium‐induced activation of high ...
Source: FEMS Yeast Research - November 9, 2014 Category: Research Authors: Linghuo Jiang, Chunlei Cao, Lilin Zhang, Wei Lin, Jing Xia, Huihui Xu, Yan Zhang Tags: Research Article Source Type: research

Physiology of the fuel ethanol strain Saccharomyces cerevisiae PE ‐2 at low pH indicates a context‐dependent performance relevant for industrial applications
This study investigates the physiology of S. cerevisiae strain PE‐2, widely used in the Brazilian fuel ethanol industry, in comparison with CEN.PK113‐7D, a reference laboratory strain, focusing on tolerance to low pH and acetic acid stress. Both strains were grown in anaerobic bioreactors, operated as batch, chemostat or dynamic continuous cultures. Despite their different backgrounds, biomass and product formation by the two strains were similar under a range of conditions (pH 5 or pH < 3, with or without 105 mM acetic acid added). PE‐2 displayed a remarkably higher fitness than CEN.PK113‐7D during batch cul...
Source: FEMS Yeast Research - October 30, 2014 Category: Research Authors: Bianca E. Della ‐Bianca, Erik Hulster, Jack T. Pronk, Antonius J.A. Maris, Andreas K. Gombert Tags: Research Article Source Type: research

Yeast tolerance to the ionic liquid 1 ‐ethyl‐3‐methylimidazolium acetate
FEMS Yeast Research,Volume 14, Issue 8, Page 1286-1294, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 28, 2014 Category: Research Source Type: research

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FEMS Yeast Research,Volume 14, Issue 8, Page 1286-1294, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 28, 2014 Category: Research Source Type: research

Comparative proteome and transcriptome analysis of lager brewer's yeast in the autolysis process
FEMS Yeast Research,Volume 14, Issue 8, Page 1273-1285, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 24, 2014 Category: Research Source Type: research

Nitrogen and carbon assimilation by Saccharomyces cerevisiae during Sauvignon blanc juice fermentation
FEMS Yeast Research,Volume 14, Issue 8, Page 1206-1222, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 24, 2014 Category: Research Source Type: research

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FEMS Yeast Research,Volume 14, Issue 8, Page 1273-1285, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 24, 2014 Category: Research Source Type: research