Frontiers of Medicine in China
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Meta-analysis of the risk factors of breast cancer concerning reproductive factors and oral contraceptive use
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Abstract The authors performed a meta-analysis of case-control studies that addressed whether reproductive factors and oral contraceptive
use were associated with breast cancer by searching the MEDLINE, PubMed, Proquest, Embase, ScienceDirect, African Healthline,
BMJ Health Intelligence and Chinese Periodical net databases for all English-language and Chinese-language papers published
from January 1, 1997 to December 31, 2007. A total of 15 studies calculating pool ORs indicated that menopausal age >50 yr
[odds ratio (OR), 1.39; 95% confidence interval (CI), 1.22–1.57] and oral contraceptive use (OR, 2....
Source: Frontiers of Medicine in China - November 10, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
A survey of physical activity among urban women in ChinaEmail this article to a colleague.
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Abstract Few studies have been conducted to assess the level of physical activity (PA) of women in China. The purpose of this study
was to describe the prevalence of PA among women in Wuhan and explore sociodemographic and behavioral correlation to PA for
the population. A total of 1359 community-dwelling women (42.97±13.60 years old) participated in this study. The long-version
IPAQ was used to define the level of PA. 45.7% of women were considered physically active, and 27.5% were vigorously active.
The proportion of physical inactivity was around 26.8%. The total and 4 domains of PA showed significant ag...
Source: Frontiers of Medicine in China - November 10, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Investigation on the mechanism of ginsenoside Rg3 in treating murine primary mammary tumorEmail this article to a colleague.
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Abstract A murine primary mammary tumor model was established to investigate the treatment with ginsenosides Rg3. The relationship
between ginsenosides Rg3 and primary mammary tumor was explored. Mammary tumor was induced by using the 7,12-dimethybenz(a)anthracene
(DMBA). Ginsenoside Rg3 was employed for treatment. The incidence of mammary tumor in every group was compared, and the expressions
of vascular endothelial growth factor (VEGF) and microvessel density (MVD) were detected by immunohistochemical method. The
cell cycle and apoptosis percentage were determined by means of flow cytometry. The incidence ...
Source: Frontiers of Medicine in China - November 7, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Expression of PC-cell-derived growth factor in breast cancerEmail this article to a colleague.
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This study is mainly aimed at evaluating the expression of PC-cell-derived growth factor (PCDGF) in breast cancer and breast
adenofibroma, and to compare with other commonly used clinical pathological indices, then to investigate the diagnostic and
targeted therapeutic purpose of PCDGF in breast cancer tissue. In this study, we detected the expression of PCDGF, p53 and
CerbB-2 in breast cancer tissue and the expression of PCDGF in breast adenofibroma tissue by immunohistochemical method, and
analyzed the relationship between them. We found that PCDGF was expressed in most breast cancer tissue, but was not in breast
ad...
Source: Frontiers of Medicine in China - November 7, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Construction and humoral immune response of Epstein-Barr virus latent membrane protein 2 DNA vaccine in miceEmail this article to a colleague.
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In conclusion, the EBV LMP2 DNA vaccine can induce a strong humoral immune response in mice.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0087-5Authors
Jianqing Pan, Huazhong University of Science and Technology Department of Internal Medicine, Tongji Hospital, Tongji Medical College Wuhan 430030 ChinaQin Zhang, Huazhong University of Science and Technology Department of Internal Medicine, Tongji Hospital, Tongji Medical College Wuhan 430030 ChinaDaowen Wang, Huazhong University of Science and Technology Department of Internal Medicine, Tongji Hospital, Tongji Medical College Wuhan 43003...
Source: Frontiers of Medicine in China - November 7, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Protective effect of tanshinone II A on signal transduction system protein kinase B in rats with myocardial hypertrophyEmail this article to a colleague.
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Abstract The effects of tanshinone II A on cell signal transduction system protein kinase B in rats with myocardial hypertrophy induced
by the abdominal aorta partial coarctation were investigated. Rat models of myocardial hypertrophy were established by using
abdominal aorta partial coarctation method. Forty-eight rats were randomly divided into sham group (S group), model group
(M group), valsartan treatment group (X group), low-dose tanshinone treatment group (LD group), medium-dose tanshinone treatment
group (MD group), and high-dose tanshinone treatment group (HD group) (n = 8 in each group). Left ventr...
Source: Frontiers of Medicine in China - November 6, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Relationship between Th17 cells and allograft rejectionEmail this article to a colleague.
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Abstract Thl7 cells, a special kind of auxiliary type T cells, can secrete IL-17A, IL-17F, IL-21, IL-22, etc., as a newly discovered
T-cell subset in recent years. As a different subset from the Thl and Th2 cells, Th17 cells play an important role in the
development of a variety of autoimmune diseases. A current study shows that the IL-6 inflammatory response of the organization
in combination with the occurrence of TGF-β can induce the differentiation of Thl7 cells. IL-23 can promote the production
of IL17, as well as participate in amplification and maintenance of the survival of IL-l7 generating cells. I...
Source: Frontiers of Medicine in China - November 6, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Epidemiological studies of women under age 30 infected with human papillomavirusEmail this article to a colleague.
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Abstract The aim of this paper was to investigate the prevalence of human papilloma virus (HPV) infection in women under 30 years old
with cervical intraepithelial neoplasia (CIN) or cervical cancer, in order to provide a basis for cervical cancer prevention
and treatment. We recruited 2052 female cases from the education system in Futian District, Shenzhen city, from April 2006
to April 2008, with age ranging from 22 to 60 years old. Secondgeneration hybrid capture test was done for detection of female
genital tract HPV and cervical colposcopy for screening CIN or cervical cancer. The prevalence of HPV and ...
Source: Frontiers of Medicine in China - November 6, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Preliminary results of gentamycin combined with sodium bicarbonate for prevention of irinotecan-induced diarrheaEmail this article to a colleague.
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Abstract The aim of this paper was to observe the clinical effect of gentamycin combined with sodium bicarbonate for the prevention
of irinotecan-induced diarrhea. A total of 98 patients with stage IV cancers were recruited and divided into a prevention
group (52 patients) and a control group (46 patients). All patients received the chemotherapy including irinotecan. The prevention
group received gentamycin and sodium bicarbonate before the use of irinotecan for 4 days; the control group did not receive
any prevention. The use of gentamycin and sodium bicarbonate resulted in significantly higher stool pH (P&...
Source: Frontiers of Medicine in China - November 6, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Expression of STAT6 and NF-κB p65 in the colon mucosa of patients with ulcerative colitisEmail this article to a colleague.
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Abstract The expression of signal transducer and activator of transcription 6 (STAT6) and nuclear factor-κB (NF-κB) in the colonic
mucosa of patients with ulcerative colitis (UC) was examined. Real-time polymerase chain reaction and immunohistochemistry
were used to detect the expression of STAT6 and NF-κB p65 at both mRNA and protein levels in the colonic mucosa of patients
with UC and healthy volunteers. The results showed that the expression levels of STAT6 and NFκB p65 in the colonic mucosa
of patients with UC were significantly higher than in normal controls at both mRNA and protein levels. These da...
Source: Frontiers of Medicine in China - November 6, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
The relationship between platelet-derived growth factor expression and angiogenesis/lymphangiogenesis in cervical cancerEmail this article to a colleague.
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Abstract This paper is aimed to examine if changes in platelet-derived growth factor (PDGF) expression at different stages of cervical
cancer are related to the variation in blood vessel density (BVD) and lymphatic vessel density (LVD) to evaluate the relationship
between PDGF expression and stages and metastasis of cervical cancer. Polymerase chain reaction (PCR) and RT-PCR were used
to detect the expression levels of PDGF in 45 cervical cancer tissue samples (the experimental group). The samples were immunohistochemically
stained with monoclonal antibodies D2-40 and CD34, and BVD and LVD were measured. The...
Source: Frontiers of Medicine in China - November 5, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effect of compound Sophorae Flavescentis Jiechangrong capsule on expression of NF-κB p65 and STAT6 in the intestinal mucosa of patients with ulcerative colitisEmail this article to a colleague.
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Abstract The effects of compound Sophorae Flavescentis Jiechangrong capsule (CSFJC) on the expression of nuclear factor-κB p65 (NF-κB
p65) and signal transducer and activator of transcription 6 (STAT6) in the intestinal mucosa of patients with ulcerative colitis
and the possible mechanism were investigated. Eighteen patients with ulcerative colitis were randomly divided into a traditional
Chinese medicine (TCM) group (n = 11) treated by CSFJC and a western medicine (WM) group (n = 7) treated by Sulfasalazine tablets. The treatment duration lasted eight weeks. Before and after the treatment, the symptoms
an...
Source: Frontiers of Medicine in China - November 5, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Mature cystic teratoma of the parotid gland: a case report and review of the literatureEmail this article to a colleague.
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We report one case of mature cystic teratoma that presented as a tender, well-defined round mass in the right parotid gland
region. Teratomas are common neoplasms but are very rarely found in the parotid gland region, so diagnosis and treatment when
such occurs is a challenge. Magnetic resonance imaging scanning is useful in determining the nature of teratomas, and only
surgery and pathology can provide a final diagnosis. The treatment strategy is to preserve both the facial nerve and the surrounding
parotid gland tissue, while completely extirpating the teratoma.
Content Type Journal ArticleCategory Case ReportDOI ...
Source: Frontiers of Medicine in China - November 5, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effect of pEGFP-survivin on GBC-SD cell growth and chemotherapy sensitivityEmail this article to a colleague.
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Abstract This paper is aimed to investigate the effect of survivin shRNA on chemotherapy resistance in human gallbladder carcinoma
GBC-SD cells. The viability of human gallbladder carcinoma GBC-SD, GBC-SD/enhanced green fluorescent protein (EGFP), and GBC-SD/survivin
cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and mRNA and protein of survivin
were tested by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. After the cells were treated with
cisplatin (DDP) (3.0 µg/mL) for the same time, cell survival rate and IC50 was detected with ...
Source: Frontiers of Medicine in China - November 5, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effect of atorvastatin on tumor growth and metastasis in a breast cancer cell xenograft model and its mechanismEmail this article to a colleague.
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Abstract This paper aims to evaluate the effects and the possible mechanisms of atorvastatin on tumor growth and metastasis in a xenograft
tumor model. Twenty-four female athymic BALB/C mice with MDA-MB-435 xenograft tumors were randomly assigned to three groups:
a control group, a low-dose atorvastatin treatment group, and a high-dose atorvastatin treatment group. The mice in the treatment
groups began to be administered with atorvastatin (10 or 20 mg/kg per day) when the xenograft tumors reached 1 cm in diameter.
At the end of the experiment, the tumor volume and weight and the lung metastasis colonies of ...
Source: Frontiers of Medicine in China - November 4, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Adenovirus-mediated tissue inhibitor of metalloproteinase-3 gene transfection inhibits rabbit intervertebral disc degeneration in vivoEmail this article to a colleague.
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Abstract The aim of this study was to investigate the inhibitory effects of recombinant adenovirus vector carrying tissue inhibitor
of metalloproteinase-3 (RAd-TIMP-3) against degeneration of rabbit intervertebral disc. Thirty Japanese white rabbits of 4
months old were randomly divided into 5 groups. Mild or moderate rabbit lumbar disc degeneration model was constructed with
the controllable axial loading device by imposing 98 N pressure at the discs for 2 weeks. Various doses of virus were injected
into the degenerated discs as follows: 20 μL of normal saline in group 1; 20 μL of RAd66 (an empty adenovir...
Source: Frontiers of Medicine in China - October 28, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effect of PRAK gene knockout on the proliferation of mouse embryonic fibroblastsEmail this article to a colleague.
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Abstract p38 regulated/activated protein kinase (PRAK) plays a key role in cell senescence and tumor suppression. The aim of this study
was to investigate if PRAK had effect on cell proliferation. The growth of PRAK
+/+ and PRAK
−/− mouse embryonic fibroblast (MEF) cells was measured by methylthiazoletetrazolium (MTT) colorimetric assay, and the proportion
of the cell number in different phases of the cell cycle was analyzed by flow cytometry. The growth curves showed that the
growth rate was notably decreased, and cell double time was elongated in PRAK
−/− cells; moreover, the number of PRAK
−/...
Source: Frontiers of Medicine in China - October 27, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Role of exercise training on insulin resistance and TNF-α in high-fat diet ratsEmail this article to a colleague.
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In conclusion,
exercise training ameliorates insulin resistance. The reduction of the level of serum TNF-α and the increased expression of
TNF-α in adipose tissue by exercise training may be involved in this mechanism.
Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11684-009-0071-0Authors
Hui Sun, Huazhong University of Science and Technology Department of Endocrinology, Union Hospital, Tongji Medical College Wuhan 430022 ChinaXiuling Deng, Huazhong University of Science and Technology Department of Endocrinology, Union Hospital, Tongji Medical College Wuhan 430022 ChinaFangxi Xiao, Huazhong Uni...
Source: Frontiers of Medicine in China - October 27, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
The role of natriuretic peptide precursor A gene polymorphism in the development of coronary heart disease in Chinese Han populationEmail this article to a colleague.
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Abstract Natriuretic peptide precursor A (NPPA) is synthesized, stored, and released by atrial myocytes. Previous studies have shown
that NPPA plays a significant role in the regulation of coronary circulation and in atherosclerosis. Rs5065 NPPA gene polymorphism
leads to the translation of NPPA with two additional arginines and has been suggested to be associated with salt-sensitive
hypertension. The purpose of the present study was to investigate the relationship between the rs5065 NPPA gene polymorphism
and the risk of coronary heart disease (CHD) in Chinese Han population. We genotyped the single nucleot...
Source: Frontiers of Medicine in China - October 22, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Primary spinal epidural non-Hodgkin’s lymphoma presented with spinal cord compression syndromeEmail this article to a colleague.
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Abstract The spinal epidural space is an uncommon presenting site in primary non-Hodgkin’s lymphomas, especially for children. A boy
suffered spinal cord compression syndrome caused by primary spinal epidural non-Hodgkin’s lymphoma. Thoracolumbar magnetic
resonance imaging (MRI) demonstrated an intraspinal mass. An operation was performed with gross total tumor removal. Histological
examination revealed a non-Hodgkin’s B-cell lymphoma. Bone marrow aspiration was negative for lymphoma involvement. No other
therapies (chemotherapy and/or radiotherapy) were performed according to the parents’ opinion. T...
Source: Frontiers of Medicine in China - October 22, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
The role of CDK1 siRNA interference in cell cycle and cell apoptosisEmail this article to a colleague.
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Abstract In the present report, cyclin-dependent kinase1 (CDK1) siRNA was transfected into cells to silence the CDK1 gene expression
and study its role in the cell cycle and cell apoptosis. The siRNA targeting CDK1 gene was chemically synthesized and transfected
into Hela cells by lipofectamine 2000. The expression levels of CDK1 gene and protein were examined by real-time quantitative
polymerase chain reaction (PCR) and Western blot, respectively. The cell cycle was analyzed by using DNA content analysis
by flow cytometry. Cell apoptosis was detected by the Annexin V/PI method. The morphological changes of ...
Source: Frontiers of Medicine in China - October 22, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Long-term remodeling of anterior alveolar bone from treatment to retentionEmail this article to a colleague.
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Abstract Bone remodeling in orthodontic treatment has been one of the most concerned issues. The purpose of this study was to characterize
the changes in bone remodeling on both labial and lingual aspects of the anterior teeth during treatment and retention. Data
of 55 extraction cases (41 females and 14 males) were collected at pretreatment, post-treatment, and follow-up stages. Bone
thickness on both labial and lingual aspects of the upper and lower incisors were measured at the level of the center of resistance
(UC and LC) and that of 3 mm apical to the upper and lower incisors’ center of resistance (UA...
Source: Frontiers of Medicine in China - October 22, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Giant renal angiomyolipoma with tuberous sclerosis complexEmail this article to a colleague.
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This article presents a case of renal AML occurring in a 14-year-old girl with a definite diagnosis of TSC. She had been diagnosed
with TSC at the age of three, and a schedule for close observation was disobeyed. At this time, she underwent a series of
examinations: physical examination, ultrasonography, angiography, computed tomography (CT), and magnetic resonance imaging
(MRI) scans. The physical examination showed adenoma sebaceum in a butterfly paranasal distribution, and a mass was palpated
in the left upper quadrant. There were no neurological deficits. Imaging studies (including ultrasonography, angiography,
CT...
Source: Frontiers of Medicine in China - October 17, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effects of phosphatidylinositol 3-kinase inhibitor on human cervical carcinoma cells in vitroEmail this article to a colleague.
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This study was to investigate the regulation of proliferation and
apoptosis by LY294002, an inhibitor of PI3K in cervical cancer cells and the expression of FLICE-like inhibitory protein (c-FLIP)
in vitro. Human cervical cancer HeLa cells were used in this experiment and cultured. The cultured cells were treated with LY294002
at different concentrations (10, 25, 50 and 100 μmol/L) for 6, 12, 24, and 48 h before harvesting for evaluation. Cell viability
was measured by 3-(4,5)-dimethylthiazol(-2-y1)-3,5-di-phenyltetrazoliumbromide (MTT) assay. Apoptosis was analyzed by flow
cytometry. The expression of c-FLIP was dete...
Source: Frontiers of Medicine in China - July 30, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Correlation between viral load and liver cirrhosis in chronic hepatitis B patientsEmail this article to a colleague.
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Abstract The aim of this paper is to investigate the relationship between hepatitis B virus (HBV) DNA levels during the course and
the progression to cirrhosis with chronic hepatitis B. A total of 239 chronic hepatitis B patients confirmed by liver biopsy
between 2001 and 2007 were followed up for a median of 28 months. Compared with the patients without cirrhosis, the patients
progressed to cirrhosis were older and with higher HBV-DNA levels at end point. However, there was no significant difference
in cirrhosis progression between different HBV-DNA groups at baseline (P = 0.531). Kaplan-Meier analysis show...
Source: Frontiers of Medicine in China - July 29, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Construction of eukaryotic expression vector of human arresten gene and its secreted expression in HEK 293 cellsEmail this article to a colleague.
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Abstract The eukaryotic expression vector of human arresten gene was constructed and its secretive expression human embryonic kidney
(HEK 293) cells was detected. Human arresten gene was amplified from recombinant plasmid pGEM-Arr by polymerase chain reaction
(PCR), and then digested with restriction endonucleases BamH I and EcoR I. The target fragment was inserted into the BamH I and EcoR I restriction sites of eukaryotic expression vector pSecTag2 to construct pST-AT. Restriction analysis and DNA sequencing
indicated that the arresten gene was successfully inserted into pSecTag2. The recombinant plasmid was...
Source: Frontiers of Medicine in China - July 25, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Sensory innervation of the anterior eye segment in rats: a retrograde tracing studyEmail this article to a colleague.
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This study aimed to investigate the sensory innervation of the anterior eye segment in rats by retrograde tracing with 1,1-dioleyl-3,3,3,3-tetramethylindocarbocyanine,
4-chlorobenesulfonate (FAST Dil) injected into the anterior chamber. The sensory innervation of distinct elements of the anterior
segment of the rat’s eye, i.e. the cornea, ciliary body, iris, and trabecular meshwork, were studied by retrograde tracing
using FAST Dil as a tracer. FAST Dil was injected into the anterior chambers of the rat’s eyes. The animals were sacrificed
at different time points, i.e., 2, 3, 4, and 5 days after the injection. FAST...
Source: Frontiers of Medicine in China - July 25, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Design of tandem genes cluster for isoflavone engineeringEmail this article to a colleague.
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Abstract In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported.
A tandem genes cluster CHS-CHI-IFS (rIFS) of secondary metabolites of plant isoflavones was constructed by using the chalcone
synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830)
in only one recombination with the pET22b vector. The resulting expression vector pET-rIFS was heterogeneously expressed and
co-incubated with barrenwort extractions, and the genistein-like component was detected.
Conten...
Source: Frontiers of Medicine in China - July 25, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effects of exercise therapy on bone mineral density in early postmenopausal women: a controlled trialEmail this article to a colleague.
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Abstract Abstract The purpose of this article is to determine the effect of a well-designed combined aerobic, resistance, and extension
exercise program on bone mineral density (BMD) in postmenopausal women. The population comprised 45 postmenopausal women,
who exercised over 12 months (exercise group), and 36 women who served as a non-training control group. BMD of the hip, and
lumbar spine was measured at the baseline and 12th month. Repeated measurement analysis of variance and nonparametric test
were utilized to compare differences between the exercise group and controls. Thirty-six out of 45 persons in ...
Source: Frontiers of Medicine in China - July 25, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Construction and expression of hepatitis B virus vector encoding TC-tagged core proteinEmail this article to a colleague.
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In this study, we constructed a recombinant
HBV vector encoding TC-tagged core protein for biarsenical labeling of HBV virion. TC tag was genetically inserted near the
immunodominant c/e1 site of HBV core protein by mutagenesis. Western blot and enzyme-linked immunosorbent assay (ELISA) analysis
showed that the TC-tagged core protein, hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) could be expressed
in cells transfected with the recombinant HBV vector, which is similar to the cells transfected with wild-type HBV vector.
Reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot a...
Source: Frontiers of Medicine in China - July 25, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of related proteinsEmail this article to a colleague.
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Abstract The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible
factor-1α (HIF-1α) were investigated. The Hela cells were divided into three groups, namely, H0 (non-transfected Hela cells), H1 (pGenesil-1 empty plasmid-transfected Hela cells), and H2 (HIF-1α-shRNA plasmid-transfected Hela cells), and were cultured under hypoxia (1% O2) and normoxia for 48 h. The expression of HIF-1α, E-cadherin, β-catenin, and actin was detected using Western blot. The scratch
test and the invasion assay were applied to examine the invasion in each group. ...
Source: Frontiers of Medicine in China - July 25, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
MicroRNAs and hepatitis virusesEmail this article to a colleague.
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This article reviews the current knowledge of viral hepatitis (types B and C) at the level of miRNA and tries
to outline areas of potential studies.
Content Type Journal ArticleCategory ReviewDOI 10.1007/s11684-009-0055-0Authors
Gang Li, The Third Affiliated Hospital of Sun Yat-sen University Department of Infectious Diseases Guangzhou 510630 ChinaXiaojia Xiong, The Third Affiliated Hospital of Sun Yat-sen University Department of Infectious Diseases Guangzhou 510630 China
Journal Frontiers of Medicine in ChinaOnline ISSN 1673-7458Print ISSN 1673-7342 (Source: Frontiers of Medicine in China)
Source: Frontiers of Medicine in China - July 22, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effect of lanthanum chloride on growth of breast cancer cells and regulation of c-met transcriptionEmail this article to a colleague.
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Abstract Lanthanum salt is a prescription drug, but its underlying functions and mechanisms are not fully understood currently. To
explore the potential therapeutic value of lanthanum chloride, cytotoxicity test was applied to investigate its effects on
cell proliferation. Furthermore, we observed its influence on pro-oncogene c-met by reverse transcription polymerase chain reaction (RT-PCR). In MCF-7 cell line, lanthanum chloride repressed cell proliferation
at high concentrations but had no significant inhibition effect on cell growth at low concentrations. However, we observed
that lanthanum chloride repr...
Source: Frontiers of Medicine in China - July 22, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Laparoscopic sigmoid vaginoplasty in women with Mayer-Rokitansky-Kuster-Hauser syndromeEmail this article to a colleague.
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Abstract The feasibility and clinical therapeutic effects of laparoscopic sigmoid vaginoplasty in women with Mayer-Rokitansky-Kuster-Hauser
syndrome (MRKHs) were explored. The records of 11 MRKHs patients who underwent laparoscopic sigmoid vaginoplasty from 2003
to 2005 were reviewed, and long-term results were evaluated by follow-up. The mean operating time was 234 min (range, 130–300
min), the mean hospital stay was 9.4 days (range, 7–15 days), and the mean hemoglobin drop was 1.91 g/dL (range, 1.6–3.2 g/dL).
A functional neovagina was created measuring 11 to 14 cm in length and two fingers in breadt...
Source: Frontiers of Medicine in China - July 21, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effect of Zhihuang decoction on CD14 expression in lipopolysaccharide signal transduction pathway of alcohol-induced liver disease in ratsEmail this article to a colleague.
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Abstract This paper aims to investigate the effects of Zhihuang (枳黄) decoction on CD14 expression in the lipopolysaccharide signal transduction pathway of alcohol-induced liver disease
in rats. Seventy-five Wistar rats were randomly divided into three groups. Ethanol (56%, weight/volumn) was intragastrically
administrated to 50 rats (14 mL/kg body weight per day) for 10 days to establish a model of alcohol-induced liver disease,
and 25 of these 50 rats were treated with Zhihuang decoction simultaneously. Liver injury was evaluated by biochemical examination. The plasma content of endotoxin was a...
Source: Frontiers of Medicine in China - July 21, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Role of nitric oxide in biological effects of vascular endothelial growth factorEmail this article to a colleague.
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Abstract To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor (VEGF) and the possible
mechanism of VEGF, the in vitro cultured vascular endothelial cells of rabbit aorta were divided into control group, VEGF-treated group and VEGF + N-nitro-L-arginine methyl ester (L-NAME)-treated group. The absorbance (A) value of vascular endothelial cells and the levels of prostaglandin (PGI2), endothelin-1 (ET-1) and von Willebrand factor (vWF) in the supernatant were observed by water-soluble tetrazolium salt
assay, radioimmunoassay and enzyme-linked immunosorbent assay. The...
Source: Frontiers of Medicine in China - July 21, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene in vitroEmail this article to a colleague.
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Abstract In order to explore the role of cyclin-dependent kinase 4 (cdk4) in neurodegenerative diseases, lentiviral-delivered RNA interference
(RNAi) was used to silence the expression of the murine cdk4 gene in vitro. Three cdk4-shRNAs of mouse and a negative sequence were designed. After synthesis and annealing, double strand oligonucleotides
were cloned into a linearized pSIH1-H1-copGFP shRNA vector. It was confirmed by polymerase chain reaction (PCR) and sequencing
that three pairs of cdk4-shRNAs and a negative shRNA were correctly inserted into the pSIH1-H1-copGFP vector. The above recombinants
were tra...
Source: Frontiers of Medicine in China - July 21, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
The value of ultrasound elastography in differential diagnosis of superficial lymph nodesEmail this article to a colleague.
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Abstract The study evaluated the value of ultrasound elastography in differentiating the benign and malignant superficial lymph nodes.
A total of 112 subjects, including 82 patients with enlarged lymph nodes and 30 healthy volunteers, were recruited. All the
subjects were examined by B-mode ultrasonography, power Doppler ultrasonography and elastography. Most of the patients were
histopathologically confirmed by needle aspiration cytology and some patients were diagnosed by clinical data combined with
follow-up findings. The sensitivity, specificity and accuracy of B-mode ultrasonography were 59.8%, 76.5% an...
Source: Frontiers of Medicine in China - July 21, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Molecular targeted therapy of gynecological malignant tumors: the development and challenge, from laboratory to clinicEmail this article to a colleague.
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Abstract More and more molecular drugs based on targeted therapy have been utilized in the treatment of gynecologic cancer, especially
in ovarian cancer. In this article, we systematically review the current targeted therapeutic trials running in clinic. Large,
randomized trials have been conducted in the treatment of ovarian cancer, endometrial cancer and cervical cancer by usingsmall
molecule, antisense, mutational gene as well as antibodies. Other planned or ongoing trials currently targeted at molecular
markers which may play important roles in gynecological carcinogenesis and progression suggest that co...
Source: Frontiers of Medicine in China - July 21, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Molecular markers and pathogenically targeted therapy in non-small cell lung cancerEmail this article to a colleague.
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Abstract Lung cancer is one of the most common human cancers and the number one cancer killer in the United States. In general, lung
cancer includes small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC), but NSCLC accounts for approximately
90% of lung cancer. The early diagnosis and therapy of lung cancer still presents a big challenge because validated screening
tools, which can improve current early detection to reduce mortality from lung cancer, do not exist. Over the last decade,
molecular genetic abnormalities have been described in NSCLC, including chromosomal aberrations, overexpressio...
Source: Frontiers of Medicine in China - July 16, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Gene and protein expression of proteinase-activated receptor-1, 2 in a murine model of acute graft vs host diseaseEmail this article to a colleague.
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Abstract Proteinase-activated receptors (PARs) are a novel subclass of seven transmembrane-spanning, G protein-coupled receptors. PAR-1
and PAR-2 are widely expressed in a variety of cells and are found to be involved in many physiological and pathological processes
including inflammation and immune response. However, little is known about the function of PAR-1, 2 in acute graft vs host
disease (GVHD). In the present study, we first detected the expression of PAR-1, 2 protein and mRNA in a murine model of acute
GVHD using the methods of immunohistochemistry, Western blot and quantitative real-time polymerase...
Source: Frontiers of Medicine in China - July 14, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Protective effects of nicotine on gamma-aminobutyric acid neurons and dopaminergic neurons in mice with Parkinson diseaseEmail this article to a colleague.
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This study aimed to investigate the protective effect of nicotine on dopaminergic neurons and its mechanisms in mice with
Parkinson disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). C57BL/6J mice were injected with MPTP
for 8 days to establish a PD model. Nicotine was given for 10 days in the nicotine therapeutic group. Animals were examined
behaviorally with the pole test and traction test. Tyrosine hydroxylase (TH) and γ-aminobutyric acid (GABA) were determined
by using the immunocytochemistry (ICC) method. The ultrastructural changes of the caudate nucleus (CN) were observed under
electr...
Source: Frontiers of Medicine in China - July 14, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Effect of pirfenidone on renal tubulointerstitial fibrosisEmail this article to a colleague.
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Abstract Renal tubulointerstitial fibrosis (TIF) is the common end stage of various chronic renal diseases, and pirfenidone (PFD) is
a novel, broad-spectrum anti-fibrotic compound but little is known about its effect and mechanism of action on renal TIF.
In this work, we employed a unilateral ureteral obstruction (UUO) rat model to investigate the apoptosis of renal tubular
epithelial cells (RTC) after PFD treatment. Thirty-five Sprague Dawley (SD) rats were randomized into three groups: sham-operated
group (n = 7), UUO group (n = 14) and PFD group (n = 14). All rats were sacrificed at day 7 or 14 after oper...
Source: Frontiers of Medicine in China - July 11, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgsEmail this article to a colleague.
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Abstract Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain
secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody
titers in the culture supernatant and ascites were 2.048×106 and 4.096×106, respectively. By applying the anti-HBs G6 mAb and horseradish peroxidase (HRP)-labeled goat anti-HBs antibody, we developed
a sandwich ELISA (defined G6m ELISA) for detecting both wild-type and immune escape mutant HBsAgs (IEM HBsAg). The assay was
performed to detect 17 spe...
Source: Frontiers of Medicine in China - July 6, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Successful infrainguinal revascularization with autologous vein for inadvertent stripping of superficial femoral artery: a case reportEmail this article to a colleague.
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Abstract A referral patient who had previously undergone varicose vein surgery was admitted as an emergency case. On admission, the
patient complained of intolerable pain, paralysis and paresthesia of the affected limb, which was characterized by acute arterial
ischemia symptoms. Color Doppler of the artery of the affected limb indicated that no blood flow signal existed in the superficial
femoral artery. During exploratory operation, we found that the right superficial femoral artery instead of the great saphenous
vein of the affected limb had been stripped and ligated. Therefore, the intact right great sap...
Source: Frontiers of Medicine in China - June 29, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Relative expression of PTTG and bFGF in oral squamous cell carcinoma and Tca8113Email this article to a colleague.
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Abstract The purpose of this study was to investigate the expression of pituitary tumor transforming gene (PTTG) and basic fibroblast
growth factor (bFGF) in oral squamous cell carcinoma (OSCC) and tongue cancer cell line Tca8113, as well as their effects
on each other. We detected PTTG protein and bFGF in OSCC tissues from 56 cases using the streptavidin-biotin peroxidase (S-P)
method; additionally, after being treated with different concentrations of anti bFGF or PTTG antibody, PTTG or bFGF expression
in Tca8113 was examined by immuno-cytochemistry. The results were as follows: (1) Positive rates of PTTG p...
Source: Frontiers of Medicine in China - June 26, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Tim-3 mRNA expression in peripheral blood lymphocytes from asthmatic patientsEmail this article to a colleague.
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Abstract The study aimed to detect the expression of the Th1-specific cell surface protein T cell Ig and mucin domain-containing molecule-3
(Tim-3) mRNA in peripheral blood lymphocytes isolated from asthmatic patients and to examine the correlation among Tim-3 mRNA,
interleukin-4 (IL-4), interferon-γ (IFN-γ) level, and FEV1/FVC (force expiratory volume in one second/forced vital capacity)
to explore the role of Tim-3 in the development and progression of asthmatic inflammation. Tim-3 mRNA expression was detected
by reverse transcription polymerase chain reaction (RT-PCR). The IL-4 and IFN-γ levels were de...
Source: Frontiers of Medicine in China - May 9, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Expression of protease-activated receptors on platelets in healthy individualsEmail this article to a colleague.
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This study aimed to investigate the expression of protease-activated receptors (PARs) on platelets in healthy individuals
and preliminarily elucidate physiological functions of PARs. Thirty healthy volunteers, who did not take any anticoagulants
and antiplatelet agents within 10 days before the examination, were recruited. Fasting venous blood (5 mL) was taken from
the medial cubital vein in each individual and platelet-rich plasma (PRP) was prepared. The expression of PAR1 mRNA and PAR4
mRNA in PRP was determined by RT-PCR analysis. The results showed that the average levels of PAR1 mRNA and PAR4 mRNA on platelets
in...
Source: Frontiers of Medicine in China - April 26, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Expression and clinical implication of PRL-1 and PRL-3 in transitional cell carcinoma of bladderEmail this article to a colleague.
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Abstract The mRNA and protein expression of phosphatase of regenerating liver 1 (PRL-1) and phosphatase of regenerating liver 3 (PRL-3)
in transitional cell carcinoma of bladder (BTCC) and normal epithelia of bladder was investigated, and the relationship between
the BTCC and pathological changes was clarified. The expression of PRL-1 and PRL-3 mRNA was detected by using reverse transcription
polymerase chain reaction (RT-PCR) in 30 cases of BTCC and 10 cases of normal bladder, and the expression of PRL-1 and PRL-3
protein was checked by using immunohistochemistry in 30 cases of BTCC and 15 cases of normal b...
Source: Frontiers of Medicine in China - April 26, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
Adenovirus-mediated antisense ERK2 gene therapy ameliorates chronic allograft nephropathy in a rat modelEmail this article to a colleague.
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Abstract To investigate the effect and underlying mechanism of adenovirus-mediated antisense ERK2 (Adanti-ERK2) gene therapy upon chronic
allograft nephropathy (CAN) of rats, male Lewis (LEW, RT11) rats received male Fisher (F344, RT11v1) renal allografts. The
recipients were divided into three groups: (1) empty control group; (2) vector control group; (3) gene therapy group. All
recipients were sacrificed for the grafts and serum analysis at the 24th week after transplantation. Morphometric analysis
was used to determine the fibrosis of grafts. Immunohistochemistry was used to detect the expression of E-Cad...
Source: Frontiers of Medicine in China - April 26, 2009 Category: Journals (General) Tags: Frontiers of Medicine in China Source Type: journals
