Genetics and Molecular Research
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535 records returned
New insights into telomeric DNA sequence (TTAGGG)n location in bat chromosomes.
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Molossidae species, Cynomops abrasus (2n = 34, fundamental number, FN = 64), Eumops auripendulus (2n = 42, FN = 62), Molossus rufus (2n = 48, FN = 64), Molossops temminckii (2n = 48, FN = 64), and Nyctinomops laticaudatus (2n = 48, FN = 64), and Phyllostomidae species, Phyllostomus discolor (2n = 32, FN = 60), have karyotypes with different chromosome and fundamental numbers, different localization of constitutive heterochromatin, and different numbers and location of nucleolar organizer regions (NORs). Fluorescence in situ hybridization with a human probe of the telomeric sequence (TTAGGG)(n) produced fluorescent sign...
Source: Genetics and Molecular Research - November 7, 2009 Category: Genetics & Stem Cells Authors: Faria KC, Marchesin SR, Moreira PR, Beguelini MR, Morielle-Versute E Tags: Genet Mol Res Source Type: journals
Cytogenetic findings in an epithelioid sarcoma with angiomatoid features. A case report.Email this article to a colleague.
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Epithelioid sarcoma is a rare, aggressive soft tissue tumor of unknown histogenesis showing predominantly epithelioid cytomorphology. We conducted a conventional and molecular cytogenetic study of a 27-year-old male with epithelioid sarcoma with angiomatoid features. Cytogenetic analysis of epithelioid sarcoma metaphase spreads by GTG-banding revealed a diploid chromosome complement with structural and numerical aberrations. Comparative genomic hybridization analysis demonstrated the amplification of 3p24-pter, 4p15.2-p16 and 18q23, while chromosome losses involved 3p13-p14, 3q24-q26.1, 9q21, and 11q21. Fluorescence in...
Source: Genetics and Molecular Research - November 5, 2009 Category: Genetics & Stem Cells Authors: Brassesco MS, Valera ET, Castro-Gamero AM, Moreno DA, Silveira TP, Mori BM, Engel EE, Scrideli CA, Tone LG Tags: Genet Mol Res Source Type: journals
FISH analysis and cytogenetic characterization of male meiotic prophase I in Acricotopus lucidus (Diptera, Chironomidae).Email this article to a colleague.
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In the chironomid Acricotopus lucidus, two cells with quite different chromosome complements arise from the last unequal spermatogonial mitosis, as a consequence of monopolar migration of the so-called germ line limited chromosomes (Ks). The cell receiving all the Ks, in addition to two sets of the regularly segregating somatic chromosomes (Ss), develops into the primary spermatocyte, while the cell getting only Ss differentiates into an aberrant spermatocyte. Only the primary spermatocyte enters meiosis. These nuclear events in the primary spermatocytes of A. lucidus during prophase I were analyzed by carmine-orcein s...
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Staiber W Tags: Genet Mol Res Source Type: journals
Buccal micronucleus frequency is associated with age in Down syndrome.Email this article to a colleague.
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We examined the frequency of micronucleus (MN) and binucleated cells in the oral mucosa of Down syndrome patients and healthy controls matched by age and gender, addressing the effect of age and family income. Down syndrome individuals had an increased number of MN (14.30 +/- 9.35 vs 4.03 +/- 1.71; P<0.001) and binucleated cells (0.97 +/- 1.3 vs 0.33 +/- 0.66; P<0.05) per 2000 cells. Micronucleus frequency of Down syndrome individuals correlated positively with age (r = 0.437; P = 0.009), and the older (>/=21) Down syndrome age group (30.8 +/- 8.4 years old) had about 2-fold more micronuclei (P </= 0.05) than d...
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Ferreira FL, Prá D, Martino-Roth MG, Garcias GL Tags: Genet Mol Res Source Type: journals
An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers.Email this article to a colleague.
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We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random decamer primers; 15 of them gave reproducible polymorphic patterns. These 15 primers produced 226 bands, of which 65% were polymorphic. A UPGMA dendrogram clearly divided the genotypes into four groups; we concluded that RAPD analysis can be used for examining genetic relatedness among blackthorn genotypes.
PMID: 19876864 [PubMed - in process] (Source: Genetics and Molecular Research)
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Erturk Y, Ercisli S, Maghradze D, Orhan E, Agar G Tags: Genet Mol Res Source Type: journals
Africanized honey bees more efficiently convert protein diets into hemolymph protein than do Carniolan bees (Apis mellifera carnica).Email this article to a colleague.
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The superiority of Africanized over European honey bees in tropical and subtropical regions of the New World is both well documented and poorly understood. As part of an effort to try to understand the process by which the displacement of European bees occurred, we examined the ability of these two types of bees and of hybrids between the two to convert natural and artificial diets into usable protein. Newly emerged bees from colonies of tropically adapted Africanized and temperate-origin Carniolan bees and first-generation hybrids between the two were caged and fed artificial and natural protein diets for six days to ...
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Cappelari FA, Turcatto AP, Morais MM, De Jong D Tags: Genet Mol Res Source Type: journals
Salt stress causes a shift in the localization pattern of germin gene expression.Email this article to a colleague.
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The response of plants to biotic and abiotic stress factors involves biochemical, physiological, morphological, and developmental changes. Salt stress has been the subject of extensive studies due to the low salt tolerance of many crop plants. Germin and germin-like gene products are known to be involved in various aspects of plant development, such as defense, embryonic development and response to biotic as well as abiotic stress, including salt. The responses of germin and germin-like genes to salt stress vary in different plants. RT-PCR and in situ RNA hybridization methods were employed to analyze quantitative chan...
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Caliskan M Tags: Genet Mol Res Source Type: journals
Concurrent sequence variation of TP53 and TP73 genes in anaplastic astrocytoma.Email this article to a colleague.
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Disruption or loss of tumor suppressor gene TP53 is implicated in the development or progression of almost all different types of human malignancies. Other members of the p53 family have been identified. One member, p73, not only shares a high degree of similarity with p53 in its primary sequence, but also has similar functions. Like p53, p73 can bind to DNA and activate transcription. Using PCR-SSCP and gene sequencing, we analyzed the TP53 and TP73 genes in a case of a grade III anaplastic astrocytoma that progressed to glioblastoma. We found a deletion of AAG at position 595-597 of TP53 (exon 6), resulting in the de...
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Anselmo NP, Rey JA, Almeida LO, Custódio AC, Almeida JR, Clara CA, Santos MJ, Casartelli C Tags: Genet Mol Res Source Type: journals
SSR analysis demonstrates that olive production in the southern Marmara region in Turkey uses a single genotype.Email this article to a colleague.
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In conclusion, as indicated by SSR analysis, Gemlik has become the standard cultivar in this region; future research should be focused on techniques to improve the production and quality of table olives and olive oil from this cultivar.
PMID: 19876868 [PubMed - in process] (Source: Genetics and Molecular Research)
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Ipek A, Barut E, Gulen H, Oz AT, Tangu NA, Ipek M Tags: Genet Mol Res Source Type: journals
Phenotypic and RAPD diversity among 80 germplasm accessions of the medicinal plant isabgol (Plantago ovata, Plantaginaceae).Email this article to a colleague.
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Plantago ovata, popularly known as isabgol, has great commercial and medicinal importance due to thin rosy white membranous seed husk. Isabgol seeds and husks have emollient, demulcent and laxative properties. We used both biometric and molecular techniques to assess the genetic variability and relatedness of 80 germplasm accessions of Plantago spp (P. ovata, P. lanceolata, and P. major) collected both from India and abroad. The range of D2 values (2.01-4890.73) indicated a very high degree of divergence among the accessions. Based on the degree of divergence, 80 accessions/genotypes were grouped into seven clusters. T...
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Singh N, Lal RK, Shasany AK Tags: Genet Mol Res Source Type: journals
Lack of association of C282Y and H63D mutations in the hemochromatosis (HFE) gene with diabetes mellitus type 2 in a case-control study of women in Brazil.Email this article to a colleague.
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Hereditary hemochromatosis is one of the most common autosomal recessive diseases; it is characterized by excess absorption of iron. Clinically, the major challenge is to diagnose increased iron deposition before irreversible tissue damage has occurred. C282Y and H63D are the main mutations related to hereditary hemochromatosis; these mutations have been reported to be associated with increased risk of developing diabetes mellitus type 2 (DM2). We investigated whether these mutations are associated with increased risk for the development of DM2 in women in Brazil. Seventy-two women with clinical diagnosis of DM2 under ...
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Gomes KB, Carvalho MG, Coelho FF, Rodrigues IF, Soares AL, Guimarães DA, Fernandes AP Tags: Genet Mol Res Source Type: journals
Potential use of molecular markers for prediction of genotypic values in hybrid maize performance.Email this article to a colleague.
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We evaluated the potential of genetic distances estimated by microsatellite markers for the prediction of the performance of single-cross maize hybrids. We also examined the potential of molecular markers for the prediction of genotypic values and the applicability of the Monte Carlo method for a correlation of genetic distances and grain yield. Ninety S(0:2) progenies derived from three single-cross hybrids were analyzed. All 90 progenies were genotyped with 25 microsatellite markers, including nine markers linked to quantitative trait loci for grain yield. The genetic similarity datasets were used for constructing ad...
Source: Genetics and Molecular Research - November 1, 2009 Category: Genetics & Stem Cells Authors: Balestre M, Von Pinho RG, Souza JC, Oliveira RL Tags: Genet Mol Res Source Type: journals
Oropharyngeal dysphagia and language delay in partial trisomy 9p: case report.Email this article to a colleague.
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We present a case report and a comparative review of clinical findings on this condition, focusing on speech-language development, cognitive abilities and swallowing evaluation. We suggest that oropharyngeal dysphagia should be further investigated, considering that pulmonary and nutritional disorders affect the survival and quality of life of the patient. As far as we know, this is the first study of a patient with partial trisomy 9p described with oropharyngeal dysphagia.
PMID: 19866432 [PubMed - in process] (Source: Genetics and Molecular Research)
Source: Genetics and Molecular Research - October 30, 2009 Category: Genetics & Stem Cells Authors: Rossi NF, Gatto AR, Cola PC, Souza DH, Moretti-Ferreira D, Giacheti CM Tags: Genet Mol Res Source Type: journals
Internal phloem in an interspecific hybrid of cassava, an indicator of breeding value for drought resistance.Email this article to a colleague.
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We examined the stem anatomy of Manihot esculenta Crantz and its hybrid with M. oligantha Pax. Cross-sections were stained with safranin-alcian blue. Manihot esculenta and its hybrid were found to have the same vascular structure in bicollateral bundles with internal phloem, which has been associated with drought resistance in other plant groups. If this association is established for cassava, it would facilitate the selection of lines that are more adapted to arid regions. This is the first report of internal phloem in this genus.
PMID: 19866433 [PubMed - in process] (Source: Genetics and Molecular Research)
Source: Genetics and Molecular Research - October 30, 2009 Category: Genetics & Stem Cells Authors: Graciano-Ribeiro D, Hashimoto DY, Nogueira LC, Teodoro D, Miranda SF, Nassar NM Tags: Genet Mol Res Source Type: journals
Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA.Email this article to a colleague.
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Low purification efficiency and incomplete characterization of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His(6) fusion protein in Escherichia coli Top10 cells. Recombinant clones were selected by growth in ampicillin-enriched medium, PCR amplification and nucleotide sequencing. The inserted cDNA was completely identified and recombinant protein synthesis was monitored by SDS-PAGE, followed by immunoblotting with mon...
Source: Genetics and Molecular Research - October 30, 2009 Category: Genetics & Stem Cells Authors: Cajazeiras JB, Melo LM, Albuquerque ES, Rádis-Baptista G, Cavada BS, Freitas VJ Tags: Genet Mol Res Source Type: journals
POPREP: a generic report for population management.Email this article to a colleague.
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Genetic variation provides a basis upon which populations can be genetically improved. Management of animal genetic resources in order to minimize loss of genetic diversity both within and across breeds has recently received attention at different levels, e.g., breed, national and international levels. A major need for sustainable improvement and conservation programs is accurate estimates of population parameters, such as rate of inbreeding and effective population size. A software system (POPREP) is presented that automatically generates a typeset report. Key parameters for population management, such as age structur...
Source: Genetics and Molecular Research - October 30, 2009 Category: Genetics & Stem Cells Authors: Groeneveld E, Westhuizen BV, Maiwashe A, Voordewind F, Ferraz JB Tags: Genet Mol Res Source Type: journals
Genetic variability and heterogeneity of Venezuelan equine encephalitis virus vector Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations of the Colombian Atlantic coast, based on microsatellite loci.Email this article to a colleague.
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In Colombia, the mosquito Ochlerotatus taeniorhynchus has been identified as an efficient vector of the epidemic-epizootic Venezuelan equine encephalitis virus. We evaluated the genetic variability and heterogeneity of this mosquito in Colombian populations using eight microsatellite DNA loci. Two hundred and ten mosquito specimens collected from seven populations of the Colombian Atlantic coast (San Bernardo del Viento, Coveñas, Cartagena, Barranquilla, Ciénaga, Dibulla, and Riohacha) were analyzed. We found five polymorphic microsatellite loci, with 19 alleles giving 62.5% polymorphism; the mean number of a...
Source: Genetics and Molecular Research - October 30, 2009 Category: Genetics & Stem Cells Authors: Bello F, Becerra V Tags: Genet Mol Res Source Type: journals
Structure characterization of human cytomegalovirus UL131A, UL130 and UL128 genes in clinical strains in China.Email this article to a colleague.
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We examined the structure of these three genes in HCMV. Eighteen low-passage clinical isolates and five non-passage strains from congenitally HCMV-infected infants in China were used to assess the structures of the UL131A, UL130, and UL128 genes and to find possible relationships between sequence polymorphism and different signs of HCMV disease. Comparisons were made between the UL131A, UL130, and UL128 genes of clinical strains and published sequences of Towne and Merlin strains. The UL131A coding region in the clinical strains was similar to that of Towne and Merlin strains, while UL130, and UL128 coding regions in the c...
Source: Genetics and Molecular Research - October 30, 2009 Category: Genetics & Stem Cells Authors: Sun ZR, Ji YH, Ruan Q, He R, Ma YP, Qi Y, Mao ZQ, Huang YJ Tags: Genet Mol Res Source Type: journals
Correlation of aberrant expression of p53, Rad50, and cyclin-E proteins with microsatellite instability in gallbladder adenocarcinomas.Email this article to a colleague.
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Gallbladder carcinoma is an uncommon, but highly malignant tumor, with poor prognostic, and diagnostic manifestations in early stages. The Indian Council of Medical Research reported increased incidence of gallbladder carcinoma in the surviving population of the Bhopal gas tragedy that involved exposure of more than 500,000 people to methyl isocyanate gas. The severity of exposure, and increased multi-systemic morbidity in the survivors stimulated us to examine the molecular changes leading to gallbladder carcinoma. Surgically resected samples (N = 40) of gallbladder carcinoma were studied for the p53, Rad50, and cycli...
Source: Genetics and Molecular Research - October 30, 2009 Category: Genetics & Stem Cells Authors: Mishra PK, Jatawa SK, Raghuram GV, Pathak N, Jain A, Tiwari A, Varshney S, Maudar KK Tags: Genet Mol Res Source Type: journals
Cytogenetic findings in an epithelioid sarcoma with angiomatoid features.A case report.Email this article to a colleague.
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Epithelioid sarcoma is a rare, aggressive soft tissue tumor of unknown histogenesis showing predominantly epithelioid cytomorphology. We conducted a conventional and molecular cytogenetic study of a 27-year-old male with epithelioid sarcoma with angiomatoid features. Cytogenetic analysis of epithelioid sarcoma metaphase spreads by GTG-banding revealed a diploid chromosome complement with structural and numerical aberrations. Comparative genomic hybridization analysis demonstrated the amplification of 3p24~pter, 4p15.2-p16 and 18q23, while chromosome losses involved 3p13-p14, 3q24-q26.1, 9q21, and 11q21. Fluorescence in...
Source: Genetics and Molecular Research - October 30, 2009 Category: Genetics & Stem Cells Authors: Brassesco MS, Valera ET, Castro-Gamero AM, Moreno DA, Silveira TP, Mori BM, Engel EE, Scrideli CA, Tone LG Tags: Genet Mol Res Source Type: journals
Assessment of the potential genotoxic risk of medicinal Tamarindus indica fruit pulp extract using in vivo assays.Email this article to a colleague.
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Tamarindus indica has been used in folk medicine as an antidiabetic, a digestive aid, and a carminative, among other uses. Currently, there is no information in the toxicology literature concerning the safety of T. indica extract. We evaluated the clastogenic and/or genotoxic potential of fruit pulp extract of this plant in vivo in peripheral blood and liver cells of Wistar rats, using the comet assay, and in bone marrow cells of Swiss mice, using the micronucleus test. The extract was administered by gavage at doses of 1000, 1500 and 2000 mg/kg body weight. Peripheral blood and liver cells from Wistar rats were collec...
Source: Genetics and Molecular Research - September 23, 2009 Category: Genetics & Stem Cells Authors: Silva FM, Leite MF, Spadaro AC, Uyemura SA, Maistro EL Tags: Genet Mol Res Source Type: journals
Glutathione S-transferase M1 and T1 polymorphism in men with idiopathic infertility.Email this article to a colleague.
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We examined GSTM1 and GSTT1 polymorphism in 233 men with idiopathic infertility seen at the Human Reproduction Service of the Federal University of Goiás from 2004-2006. Genotype GSTM1/T1 (null) was found in 30/105 normal individuals and in 64/128 abnormal individuals, indicating a significant association with idiopathic male infertility. The sperm alteration associated with greater GSTM1/T1 (null) frequency was decreased sperm count (oligozoospermy), which was more frequent in patients with GSTM1 (78.9%) as well as in those who had GSTT1 (73.7%), although the difference was not significant. Individuals polymorphic fo...
Source: Genetics and Molecular Research - September 23, 2009 Category: Genetics & Stem Cells Authors: Finotti AC, Costa E Silva RC, Bordin BM, Silva CT, Moura KK Tags: Genet Mol Res Source Type: journals
Karyoevolution of the toadfish Thalassophryne nattereri (Batrachoidiformes: Batrachoididae).Email this article to a colleague.
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The Batrachoididae includes some venomous brackish and marine fish found in the Atlantic, Indian and Pacific oceans. This family is composed of 69 species, distributed among 19 genera. Species of the genus Thalassophryne have been reported along the coast of Rio Grande do Norte (Brazil); T. nattereri has been responsible for a large number of human injuries. Little is known about the cytogenetic features of this family. We made a karyotypic characterization of T. nattereri collected from the estuary of the Apodi/Mossoró River, using conventional Giemsa staining, C-banding and silver nitrate-nucleolar organizer reg...
Source: Genetics and Molecular Research - September 23, 2009 Category: Genetics & Stem Cells Authors: Costa GW, Molina WF Tags: Genet Mol Res Source Type: journals
RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey.Email this article to a colleague.
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The fig tree (Ficus carica L.) is of significant socio-economic importance in Turkey, with 25% of the world's fig production. Genetic variation and relationships among 14 wild-grown figs sampled from Coruh Valley in Turkey were characterized by random amplified polymorphic DNA (RAPD). Ninety-eight DNA fragments were scored after amplification of DNA samples with 13 random primers; 70% of the scored bands were polymorphic. Genetic distances between the fig genotypes ranged from 0.21 to 0.62. Genotypes 08-ART-02 and 08-ART-06 were found to be the most closely related, whereas 08-ART-09 and 08-ART-10 were the most distant...
Source: Genetics and Molecular Research - September 23, 2009 Category: Genetics & Stem Cells Authors: Akbulut M, Ercisli S, Karlidag H Tags: Genet Mol Res Source Type: journals
Gene silencing during development of in vitro-produced female bovine embryos.Email this article to a colleague.
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In conclusion, male and female embryos express different levels of X-chromosome genes and failures of these genes that are essential for development could reduce the viability of females. Nuclear transfer can modify this relation, possibly due to epigenetic memory, leading to frequent failures in nuclear reprogramming.
PMID: 19768674 [PubMed - as supplied by publisher] (Source: Genetics and Molecular Research)
Source: Genetics and Molecular Research - September 23, 2009 Category: Genetics & Stem Cells Authors: Merighe GK, Biase FH, Santos-Biase WK, Miranda MS, de Bem TH, Watanabe YF, Meirelles FV Tags: Genet Mol Res Source Type: journals
Genetics of quantitative and qualitative traits of isabgol (Plantago ovata).Email this article to a colleague.
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Isabgol is a medicinal plant known for its high-quality dietary fiber. The genetics and inheritance of economic characters, such as number of panicles, panicle length, seed yield, and swelling factor, were measured by diallel analysis of F(1) progenies from seven parents. The additive component of genetic variance was significant for days to flowering, plant height, branches/plant, peduncle length, panicle length, days to maturity, and swelling factor, whereas the dominance component of genetic variance was significant for all the characters except panicles/plant. Additivity was not significant for all the characters, ...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Singh N, Lal RK Tags: Genet Mol Res Source Type: journals
Genetic distances between popcorn populations based on molecular markers and correlations with heterosis estimates made by diallel analysis of hybrids.Email this article to a colleague.
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Diallel analysis was used to obtain information on combining ability, heterosis, estimates of genetic distances by random amplified polymorphic DNA (RAPD) and on their correlations with heterosis, for the popcorn varieties RS 20, UNB2, CMS 43, CMS 42, Zélia, UEM J1, UEM M2, Beija-Flor, and Viçosa, which were crossed to obtain all possible combinations, without reciprocals. The genitors and the 36 F(1) hybrids were evaluated in field trials in Maringá during two growing seasons in a randomized complete block design with three replications. Based on the results, strategies for further studies were develope...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Munhoz RE, Prioli AJ, Amaral Júnior AT, Scapim CA, Simon GA Tags: Genet Mol Res Source Type: journals
In vitro regeneration of cocona (Solanum sessiliflorum, Solanaceae) cultivars for commercial production.Email this article to a colleague.
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Cocona (Solanum sessiliflorum Dunal) is a solanaceous shrub native to the Amazon region that produces an edible fruit. This species has numerous advantages, particularly a high nutritional value and productivity. However, due to irregular germination and rapid loss of seed viability, there are few plantations for production on a large scale. Development of alternative propagation strategies is essential for the production of homogeneous seedlings of genotypes with superior agronomic performance. We developed techniques for in vitro regeneration of the cocona varieties Santa Luzia and Thaís for large-scale producti...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Schuelter AR, Grunvald AK, Amaral Júnior AT, da Luz CL, Luz CL, Gonçalves LM, Stefanello S, Scapim CA Tags: Genet Mol Res Source Type: journals
Leaf carbon assimilation and molecular phylogeny in Cattleya species (Orchidaceae).Email this article to a colleague.
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We examined leaf CO(2) assimilation and how it varied among species within the orchid genus Cattleya. Measurements of CO(2) assimilation and maximum quantum yield of PS II (Fv/Fm) were made for mature leaves of nine species using a portable system for photosynthesis measurement and a portable fluorometer. Leaf area was measured with an area meter, and the specific leaf mass was determined. DNA of nine Cattleya species and two species of Hadrolaelia was extracted using the CTAB protocol. Each sample was amplified and sequenced using primers for the trnL gene. The phylogenetic analyses, using neighbor-joining and maximum par...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Andrade-Souza V, Almeida AA, Corrêa RX, Costa MA, Mielke MS, Gomes FP Tags: Genet Mol Res Source Type: journals
Generation of a preliminary bovine gene atlas, using expression clustering to annotate gene function.Email this article to a colleague.
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We examined bovine gene expression in 27 tissues using non-normalized cDNA library sequencing. Contigs were generated from expressed sequence tags whose sequences overlapped. Contigs containing a minimum of five expressed sequence tags were ordered via a hierarchical clustering process, where the distance between the contigs represents their expression pattern similarity across tissues. Gene ontology terms associated with the genes in each cluster showed that co-clustered genes encoded proteins involved in a common biological process. This process can be used to annotate genes of unknown function in the cluster. Gene expre...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Keane OM, Maqbool N, McCulloch AF, McEwan JC, Dodds KG Tags: Genet Mol Res Source Type: journals
Matrix metalloproteinase gene polymorphisms: lack of association with chronic obstructive pulmonary disease in a Brazilian population.Email this article to a colleague.
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There are many candidate genes for chronic obstructive pulmonary disease (COPD). One such candidate is the group of genes that code for matrix metalloproteinases (MMPs), which play an essential role in tissue remodeling and repair associated with COPD. We tested the hypothesis that polymorphic variation in MMP genes influences the risk of developing COPD by examining functional polymorphisms in the promoters of MMP-3, MMP-9 and MMP-12 genes in 111 COPD patients and 101 controls. The -1171 5A/6A MMP-3, -1562 C/T MMP-9 and -82 A/G MMP-12 polymorphisms were analyzed by polymerase chain reaction, followed by restriction di...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Schirmer H, Basso da Silva L, Teixeira PJ, Moreira JS, Moreira AL, Simon D Tags: Genet Mol Res Source Type: journals
Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins.Email this article to a colleague.
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Quantitative and qualitative relationships were found between secreted proteins and their activity, and the hyphal morphology of Moniliophthora perniciosa, the causal agent of witches' broom disease in Theobroma cacao. This fungus was grown on fermentable and non-fermentable carbon sources; significant differences in mycelial morphology were observed and correlated with the carbon source. A biological assay performed with Nicotiana tabacum leaves revealed that the necrosis-related activity of extracellular fungal proteins also differed with carbon source. There were clear differences in the type and quantity of the sec...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Alvim FC, Mattos EM, Pirovani CP, Gramacho K, Pungartnik C, Brendel M, Cascardo JC, Vincentz M Tags: Genet Mol Res Source Type: journals
Amarelinha do Amapá: a carotenoid-rich cassava cultivar.Email this article to a colleague.
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Amarelinha do Amapá: a carotenoid-rich cassava cultivar.
Genet Mol Res. 2009;8(3):1051-5
Authors: Nassar NM, Fernandes PC, Melani RD, Pires Júnior OR
Eight cassava (Manihot esculenta Crantz) cultivars, of which four are indigenous to the Amazon region, along with an interspecific hybrid, were assessed for their carotenoid content. They were propagated and are maintained in the living Manihot collection at the University of Brasília. The cultivar "Amarelinha do Amapá", which was collected from the State of Amapá, was found to have a very high content of b-carotene: 27 mg per 100 mg, whic...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Nassar NM, Fernandes PC, Melani RD, Pires Júnior OR Tags: Genet Mol Res Source Type: journals
Letter to the Editor Comments to the paper by Ambrósio CE, Fadel L, Gaiad TP, Martins DS, et al. [Identification of three distinguishable phenotypes in golden retriever muscular dystrophy (Genet. Mol. Res. 2009 Apr 7;8 (2): 389-396)].Email this article to a colleague.
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Letter to the Editor Comments to the paper by Ambrósio CE, Fadel L, Gaiad TP, Martins DS, et al. [Identification of three distinguishable phenotypes in golden retriever muscular dystrophy (Genet. Mol. Res. 2009 Apr 7;8 (2): 389-396)].
Genet Mol Res. 2009;8(3):818-821
Authors: Zucconi E, Jazedje T, Valadares MC, Zatz M
PMID: 19731203 [PubMed - as supplied by publisher] (Source: Genetics and Molecular Research)
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Zucconi E, Jazedje T, Valadares MC, Zatz M Tags: Genet Mol Res Source Type: journals
Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle.Email this article to a colleague.
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The myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue and Blond'Aquitaine, and others) show polymorphisms in this gene; these polymorphisms are directly related to the double muscling phenotype. We looked for polymorphisms in the Nellore cattle myostatin gene and compared them with those known for taurine breeds. Seven regions, covering...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Grisolia AB, D'Angelo GT, Porto Neto LR, Siqueira F, Garcia JF Tags: Genet Mol Res Source Type: journals
Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini).Email this article to a colleague.
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Scaptotrigona xanthotricha has a wide geographic distribution in the Brazilian Atlantic rainforest. One population from southeast and two from northeast Brazil were analyzed and were found to have chromosome polymorphisms. Although the chromosome number 2n = 34 is conserved in this species, karyotypic analysis revealed clear differences between the three populations. Congruent and ubiquitous multiple nucleolus organizer regions, heterochromatin and CMA(3)-positive blocks were found. The variations suggest that this species is in a process of genetic differentiation. This differentiation process might have been enhanced...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Duarte OM, Martins CC, Waldschmidt AM, Costa MA Tags: Genet Mol Res Source Type: journals
Behavior and viability of spontaneous oxidative stress-resistant Lactococcus lactis mutants in experimental fermented milk processing.Email this article to a colleague.
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Previously, we isolated two strains of spontaneous oxidative (SpOx2 and SpOx3) stress mutants of Lactococcus lactis subsp cremoris. Herein, we compared these mutants to a parental wild-type strain (J60011) and a commercial starter in experimental fermented milk production. Total solid contents of milk and fermentation temperature both affected the acidification profile of the spontaneous oxidative stress-resistant L. lactis mutants during fermented milk production. Fermentation times to pH 4.7 ranged from 6.40 h (J60011) to 9.36 h (SpOx2); V(max) values were inversely proportional to fermentation time. Bacterial counts...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Oliveira MN, Almeida KE, Damin MR, Rochat T, Gratadoux JJ, Miyoshi A, Langella P, Azevedo V Tags: Genet Mol Res Source Type: journals
Assessing potential miRNA targets based on a Markov model.Email this article to a colleague.
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At present, studies on microRNA mainly focus on the identification of microRNA genes and their mRNA targets. Although researchers have identified many microRNA genes, relatively few microRNA targets have been identified by experimental methods. Computational programs designed for predicting potential microRNA targets provide numerous targets for experimental validation. We used a Markov model to examine base-pairing binding patterns of known microRNA targets. Using this model, potential microRNA targets in human species predicted by four well-known computational programs were assessed. Each potential target was assigne...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Fu HY, Xue DY, Zhang XD, Yang PY Tags: Genet Mol Res Source Type: journals
Effect of intracellular Wolbachia on interspecific crosses between Drosophila melanogaster and Drosophila simulans.Email this article to a colleague.
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We examined the influence of this bacterium on reproductive isolation in interspecific crosses between Drosophila melanogaster and D. simulans. We found that Wolbachia infection negatively affected these two species in homospecific crosses. However, in interspecific crosses, it only influenced sexual isolation, as infected females more frequently hybridized than females free of infection; postzygotic reduction of fitness (bidirectional cytoplasmic incompatibility) was not detected. This would be explained by the existence of several modes of rescue systems in these two species, reducing cytoplasmic incompatibility between ...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Gazla IN, Carracedo MC Tags: Genet Mol Res Source Type: journals
Comparison of coefficients and distance measurements in passion fruit plants based on molecular markers and physicochemical descriptors.Email this article to a colleague.
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We investigated seven distance measures and 14 simi-larity coefficients in a set of observations of variables of the 'yellow' passion fruit plant (Passiflora edulis Sims), submitted to multivari-ate analyses (distance, projection and grouping). Fourteen genotypes were characterized, based on DNA amplification with 16 random am-plified polymorphic DNA primers and the assessment of nine fruit physical-chemical descriptors. The distance measurements and the similarity coefficients were compared by the Spearman correlation test, projection in two-dimensional space and grouping efficiency, using five grouping methods; the g...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Cerqueira-Silva CB, Cardoso-Silva CB, Conceição LD, Nonato JV, Oliveira AC, Corrêa RX Tags: Genet Mol Res Source Type: journals
Genetic characterization of green bean (Phaseolus vulgaris) genotypes from eastern Turkey.Email this article to a colleague.
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Green bean genotypes collected from eastern Turkey were characterized using simple sequence repeat (SSR) markers and morphological traits. Among 12 SSR markers, 10 produced successful amplifications and revealed DNA polymorphisms that were subsequently used to assess genetic relatedness of the genotypes. Based on the number of alleles generated and the probability of identity values, the most informative SSR loci were PVGLND5, PVMEIG, PV-ag001, and PV-ag004. Probably, due to the inbreeding nature of beans, the heterozygosity observed within genotypes was low at most of the SSR loci. The UPGMA dendrogram constructed bas...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Sarıkamışş G, Yaşar F, Bakır M, Kazan K, Ergül A Tags: Genet Mol Res Source Type: journals
Origin of a polyploid accession of Brachiaria humidicola (Poaceae: Panicoideae: Paniceae).Email this article to a colleague.
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Brachiaria humidicola, a species adapted to poorly drained and infertile acid soils, is widely used throughout the tropics. Cytological characterization of 54 accessions of B. humidicola for breeding purposes revealed 2n = 36, 42, and 54 chromosomes. One accession (H030), with 2n = 42 chromosomes, showed a different meiotic behavior. In most accessions from the genus Brachiaria previously studied, the basic chromosome number is x = 9, but the putative basic number in H030 appears to be x = 6. Since six univalent chromosomes were found in diakinesis and metaphase I, and these behaved as laggards in anaphase I, it was hy...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Boldrini KR, Micheletti PL, Gallo PH, Mendes-Bonato AB, Pagliarini MS, Valle CB Tags: Genet Mol Res Source Type: journals
A genomic parasite in the evolution of metazoan development.Email this article to a colleague.
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It is a challenge to understand how development emerged as a mechanism to dismantle and dismiss the intromission of foreign parasites in order to consolidate a higher-level multicellular unit of selection where more heritable variations in fitness, required for complex organization, can be procured. Levels in biological hierarchy genes, networks of genes, chromosomes, cells, organisms, etc., possess heritable variations in fitness to varying degrees, and as such, they function as units of selection in the evolutionary process [Lewontin, (1970). The units of selection. Annu. Rev. Ecol. Syst. 1: 1-18]. To proceed from ea...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Hoenigsberg HF, Sanabria C Tags: Genet Mol Res Source Type: journals
Genetic anomalies in patients with severe oligozoospermia and azoospermia in eastern Turkey: a prospective study.Email this article to a colleague.
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We examined the frequency and type of both chromosomal abnormalities and Y chromosome microdeletions in 90 patients with severe male factor infertility and 75 fertile control men. Thirty of the infertile patients had nonobstructive azoospermia, 30 had oligozoospermia and 30 had normozoospermia. Five of 30 were azoospermic, four of 30 were oligozoospermic and two of 30 were normozoospermic with Y chromosome microdeletions. The AZFc locus was the most frequently deleted region (64%). Ten cases with azoospermia, four cases with oligozoospermia and four cases with normozoospermia had chromosomal abnormalities. The 75 men with ...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Ceylan GG, Ceylan C, Elyas H Tags: Genet Mol Res Source Type: journals
The radiotracer (99m)Tc-MIBI is not genotoxic for human peripheral blood lymphocytes at diagnostic radioactive dose.Email this article to a colleague.
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The radiotracer technetium-99m methoxyisobutyl isonitrile ((99m)Tc-MIBI) has been widely used for myocardial blood flow imaging. We investigated the genotoxicity of (99m)Tc-MIBI in cultured human lymphocytes at the same concentration used in patients. Radioactivity doses were determined in whole blood at 5 min post-injection of 20 mCi (99m)Tc-MIBI in patients. Subsequently, whole blood of human volunteers was incubated with 1, 2.3, 4 or 8 microCi (99m)Tc-MIBI. After a 30-min incubation, the lymphocytes were stimulated with a mitogen to assay for micronuclei in cytokinesis-blocked binucleated cells. The frequency of mic...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Hosseinimehr SJ, Ahmadi A, Beiki D, Mahmoudzadeh A, Babaei M, Habibi E Tags: Genet Mol Res Source Type: journals
Proteomics-based approach for identification and purification of human phosphate binding apolipoprotein from amniotic fluid.Email this article to a colleague.
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Human amniotic fluid is of both maternal and fetal origin; it protects the fetus and provides the environment for growth and development of the fetus. We used a proteomics-based approach for targeting and purifying human phosphate binding protein, a member of the DING family of proteins from amniotic fluid, using Blue Sepharose CL-6B, DEAE-Sephacel and gel filtration chroma-tography. The protein had earlier been reported to be serendipitously purified along with PON1 (paraoxonase 1). It was identified using electro-spray-ionization-time-of-flight mass spectrometry and was found to be human phosphate binding protein. Hu...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Alam M, Mahajan M, Raziuddin M, Singh TP, Yadav S Tags: Genet Mol Res Source Type: journals
Mutagenic potential of water from Pelotas Creek in Rio Grande do Sul, Brazil.Email this article to a colleague.
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Water resource degradation is one of mankind's greatest worries, as it causes direct and indirect damage to the associated biota. We initiated a water monitoring study in Pelotas Creek in 2003 in order to assess the mutagenic effect of the creek's waters. Allium cepa cells exposed to water samples and a chronically exposed macrophyte were analyzed, through evaluation of the mitotic index, mitotic anomalies, interphase anomalies, and total anomalies. Five points were chosen along the lower course of Pelotas Creek, from which water samples and floating pennywort (Hydrocotyle ranunculoides, Apiaceae) were collected in 200...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Santos TC, Maciel LF, Leal KS, Bender AE, Paiva TS, Garcias GL, Martino-Roth MG Tags: Genet Mol Res Source Type: journals
Investigation of mutations associated with pyrethroid resistance in populations of the New World Screwworm fly, Cochliomyia hominivorax (Diptera: Calliphoridae).Email this article to a colleague.
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Cochliomyia hominivorax larvae are known for their parasitic habit in living vertebrates, causing considerable economic losses to livestock industry. This ectoparasite has been controlled mainly by applying insecticides, but this method usually results in the selection of resistant individuals. The resistance mechanism known as knockdown resistance (kdr) is a generic term for amino acid substitutions in the sodium channel associated with pyrethroid resistance, and substitutions in residue 251 of the carboxylesterase E3 have been associated with organophosphate and pyrethroid hydrolysis. We looked for L1014F kdr and W25...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: da Silva NM, de Azeredo-Espin AM Tags: Genet Mol Res Source Type: journals
Short CommunicationNew insights into telomeric DNA sequence (TTAGGG)(n) location in bat chromosomes.Email this article to a colleague.
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Molossidae species, Cynomops abrasus (2n = 34, fundamental number, FN = 64), Eumops auripendulus (2n = 42, FN = 62), Molossus rufus (2n = 48, FN = 64), Molossops temminckii (2n = 48, FN = 64), and Nyctinomops laticaudatus (2n = 48, FN = 64), and Phyllostomidae species, Phyllostomus discolor (2n = 32, FN = 60), have karyotypes with different chromosome and fundamental numbers, different localization of constitutive heterochromatin, and different numbers and location of nucleolar organizer regions (NORs). Fluorescence in situ hybridization with a human probe of the telomeric sequence (TTAGGG)(n) produced fluorescent sign...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Faria KC, Marchesin SR, Moreira PR, Beguelini MR, Morielle-Versute E Tags: Genet Mol Res Source Type: journals
Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera, Apidae) in the State of Santa Catarina, Brazil.Email this article to a colleague.
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The mite Varroa destructor is an ectoparasite that is considered a major pest for beekeeping with European honey bees. However, Africanized bee colonies are less threatened by this ectoparasite, because infestation levels remain low in these bees. The low reproductive ability of female mites of the Japanese biotype (J), introduced to Brazil early in the 1970s was initially considered the main factor for the lack of virulence of this parasite on Africanized bees. In other regions of the world where the Korean (K) biotype of this mite was introduced, there have been serious problems with Varroa due to the high reproducti...
Source: Genetics and Molecular Research - September 6, 2009 Category: Genetics & Stem Cells Authors: Strapazzon R, Carneiro FE, Guerra JC, Moretto G Tags: Genet Mol Res Source Type: journals
