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Cardiac rather than intestinal-type background in endoscopic resection specimens of minute Barrett adenocarcinomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
I read with interest the article by Takubo et al . This collaborative work between Japanese and German workers reported that more than 70% of their cases showed cardiac/fundic-type mucosa adjacent to the tumors studied and proposed it seems better to define Barrett esophagus as metaplastic columnar-lined esophagus alone, without requiring the presence of goblet cells. (Source: Human Pathology)
Source: Human Pathology - November 13, 2009 Category: Pathology Authors: Olorunda Rotimi Tags: Correspondence Source Type: journals

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(Source: Human Pathology)
Source: Human Pathology - November 13, 2009 Category: Pathology Source Type: journals

ContentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Human Pathology)
Source: Human Pathology - November 13, 2009 Category: Pathology Source Type: journals

Information for AuthorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Human Pathology)
Source: Human Pathology - November 13, 2009 Category: Pathology Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Human Pathology)
Source: Human Pathology - November 13, 2009 Category: Pathology Source Type: journals

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(Source: Human Pathology)
Source: Human Pathology - November 13, 2009 Category: Pathology Source Type: journals

Overexpression of heat shock protein 27 in squamous cell carcinoma of the uterine cervix—replyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We thank Drs El-Ghobashy and Herrington for their interest in our study . First of all, we did not assert that HPV infection up-regulates Hsp27 expression in cervical intraepithelial neoplasia and squamous cell carcinoma. Although this might be one of the possible mechanisms underlying Hsp27 overexpression in neoplastic cervical lesions, we clearly have mentioned that “further investigations are necessary to test this hypothesis” . As El-Ghobashy and Herrington have suggested in the letter, comparison of expression levels of Hsp27 and p16 in various cervical lesions, including squamous metaplasia, would be helpful to ...
Source: Human Pathology - October 20, 2009 Category: Pathology Authors: Akiko Ono, Hirotaka Koizumi Tags: Correspondence Source Type: journals

Drug reaction with eosinophilia and systemic symptoms: a cause of human herpesvirus 6–related fulminant myocarditis and hepatitis in immunocompetent patients—replyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The letter by Eshki is certainly relevant to our report and further illustrates the importance of the awareness of human herpesvirus 6 (HHV-6) reactivation in medical practice. The role of HHV-6 reactivation in drug reaction with eosinophilia and systemic symptoms (DRESS) is currently under debate. As stated in our report, the trigger event for our patient was indeterminate. Drug-induced or non–drug-related hypersensitivity syndrome is neither confirmed nor ruled out. Extensive drug and toxicology screening tests and medical history inquiry through several involved physicians and close acquaintances were conducted; the f...
Source: Human Pathology - October 20, 2009 Category: Pathology Authors: Yilan L. Chang Tags: Correspondence Source Type: journals

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(Source: Human Pathology)
Source: Human Pathology - October 20, 2009 Category: Pathology Source Type: journals

Table of ContentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Human Pathology)
Source: Human Pathology - October 20, 2009 Category: Pathology Source Type: journals

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(Source: Human Pathology)
Source: Human Pathology - October 20, 2009 Category: Pathology Source Type: journals

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Source: Human Pathology - October 20, 2009 Category: Pathology Source Type: journals

Overexpression of YKL-40 is an independent prognostic marker in gastric cancerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the expression of YKL-40 was studied by immunohistochemistry in gastric cancer tissue microarray containing 172 primary gastric cancer cases and 70 adjacent nonneoplastic mucosa specimens. The correlations between YKL-40 expression and clinicopathologic features, as well as activation of PI3K/Akt pathways were addressed. Expression of YKL-40 was significantly higher in gastric cancer tissues than that in adjacent nonneoplastic tissues. Overexpression YKL-40 was found in 28.4% of gastric cancers and was significantly associated with tumor invasion (P = .007) and lymph node metastasis (P = .009). For survival ...
Source: Human Pathology - September 18, 2009 Category: Pathology Authors: Jiong Bi, Sze-Hang Lau, Zi-Li Lv, Dan Xie, Wen Li, Ying-Rong Lai, Jue-Min Zhong, Hui-qun Wu, Qiao Su, Yu-long He, Wen-Hua Zhan, Jian-Ming Wen, Xin-Yuan Guan Tags: Original Contributions Source Type: journals

Drug reaction with eosinophilia and systemic symptoms: a cause of human herpesvirus 6–related fulminant myocarditis and hepatitis in immunocompetent patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
I read with great interest the article by Chang et al . These authors reported a case of human herpesvirus 6 (HHV-6)–related fulminant myocarditis and hepatitis in an immunocompetent patient with a fatal outcome. (Source: Human Pathology)
Source: Human Pathology - September 16, 2009 Category: Pathology Authors: Vincent Descamps Tags: Correspondence Source Type: journals

Hep G2 is a hepatoblastoma-derived cell lineEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hepatoblastoma (HB) is an embryonal malignancy of hepatocellular origin and the most common primary liver tumor of childhood, often presenting in the first years of life . Pediatric hepatocellular carcinoma is a rare tumor associated with much worse prognosis and aggressive behavior than HB and significantly less responsive to chemotherapy. It may occur as early as 1 year of age and in children may not be preceded by cirrhosis. Distinguishing between these tumors is sometimes difficult, especially in older children, so our laboratory is developing molecular genetic tests and has used the well-established cell line Hep G2 f...
Source: Human Pathology - September 14, 2009 Category: Pathology Authors: Dolores López-Terrada, Sau Wai Cheung, Milton J. Finegold, Barbara B. Knowles Tags: Correspondence Source Type: journals

E-cadherin in solid pseudopapillary tumors of the pancreas-ReplyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We appreciate the comments of Dr Chetty regarding our manuscript and agree with the notion that the immunohistochemical staining pattern of E-cadherin in solid pseudopapillary neoplasm (SPN) is dependent on the type of antibody used . The immunohistochemical staining pattern of E-cadherin seems to be determined by which domain is recognized by the antibody. Like the results by Chetty et al, we found that nuclear expression of E-cadherin in 53 (94.7%) of 56 SPN cases was shown using clone 36 directed against the cytoplasmic domain of E-cadherin (BD Transduction Laboratories, Franklin Lakes, NJ, USA) , whereas none of 50 cas...
Source: Human Pathology - September 14, 2009 Category: Pathology Authors: Eunsil Yu Tags: Correspondence Source Type: journals

Tubular invasion and the morphogenesis of tumor budding in colorectal carcinomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The epithelial-mesenchymal transition (EMT) is a hot topic in tumor biology that, with the recognition of tumor budding as a prognostic factor, has found its way into the surgical pathology of colorectal cancer. As a morphogenetic program of embryonic gastrulation governed by WNT/β-catenin signaling, in executing the EMT, epithelia dissociate and migrate as fibroblast-like cells. Phenotypic similarities, particularly when highlighting tumor budding by pancytokeratin immunohistochemistry, are so striking that researchers and pathologists seem to fall prey to the temptation of assuming that the biology would be the same . W...
Source: Human Pathology - September 14, 2009 Category: Pathology Authors: Friedrich Prall, Christiane Ostwald, Micheal Linnebacher Tags: Correspondence Source Type: journals

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(Source: Human Pathology)
Source: Human Pathology - September 14, 2009 Category: Pathology Source Type: journals

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(Source: Human Pathology)
Source: Human Pathology - September 14, 2009 Category: Pathology Source Type: journals

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Source: Human Pathology - September 14, 2009 Category: Pathology Source Type: journals

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Source: Human Pathology - September 14, 2009 Category: Pathology Source Type: journals

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Source: Human Pathology - September 14, 2009 Category: Pathology Source Type: journals

Overexpression of heat shock protein 27 in squamous cell carcinoma of the uterine cervixEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We read with interest the paper by Ono et al in which they describe the analysis of hsp27 expression in squamous lesions of the cervix. Using a proteomic screen, they identified hsp27 to be up-regulated in squamous cell carcinomas by comparison with normal squamous epithelium. They went on to show that up-regulation of this protein was also present in cervical intraepithelial neoplasia (CIN), the level of expression correlating with CIN grade. From their Fig. 5, hsp27 expression in CIN appears to occur in regions of the epithelium occupied by basaloid cells. This suggests that expression is not related to productive human ...
Source: Human Pathology - September 10, 2009 Category: Pathology Authors: Alaa A. El-Ghobashy, C. Simon Herrington Tags: Correspondence Source Type: journals

Novel intronic germline FLCN gene mutation in a patient with multiple ipsilateral renal neoplasmsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe here a 64-year-old man with a novel germline mutation in the FLCN gene who presented with 3 phenotypically distinct renal tumors in the same kidney, which were histologically classified as oncocytoma (1.4 cm), oncocytic papillary carcinoma (0.5 cm), and clear cell renal carcinoma (0.8 cm). Genetic analysis of normal kidney tissue revealed a heterozygous germline FLCN mutation (intron 9, IVS9+6 C>T). Additional molecular genetic testing revealed somatic mutations and epigenetic events in genes typically associated with these specific histologic tumor types: oncocytoma harbored a second FLCN mutation (intron 12, ...
Source: Human Pathology - September 7, 2009 Category: Pathology Authors: Zoran Gatalica, Stan L. Lilleberg, Semir Vranic, Eduardo Eyzaguirre, Eduardo Orihuela, Gopalrao Velagaleti Tags: Case Studies Source Type: journals

9p21 index as estimated by dual-color fluorescence in situ hybridization is useful to predict urothelial carcinoma recurrence in bladder washing cytologyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary: Recent studies have shown that chromosome 9p21 locus is frequently deleted in the early stages of urothelial carcinogenesis. To study the predictive value of the 9p21 aberrations in recurrence of urothelial carcinoma of the urinary bladder, we applied dual-color fluorescence in situ hybridization for 9p21 and chromosome 9 centromere to the bladder washing cytology samples that were obtained from the patients with urothelial carcinoma of the urinary bladder treated by transurethral resection. For the evaluation, the 9p21 index was defined as the ratio of the mean number of 9p21 signals per nucleus for that of the c...
Source: Human Pathology - September 7, 2009 Category: Pathology Authors: Shigeto Kawauchi, Hajime Sakai, Kenzo Ikemoto, Satoshi Eguchi, Motonao Nakao, Hirofumi Takihara, Tomoyuki Shimabukuro, Tomoko Furuya, Atsunori Oga, Hideyasu Matsuyama, Mutsuo Takahashi, Kohsuke Sasaki Tags: Original Contributions Source Type: journals

Large cell change of hepatocytes in chronic viral hepatitis represents a senescent-related lesionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigated whether large cell change in chronic viral hepatitis reflects cellular senescence because of morphological similarities between the 2 conditions. The expression of markers of senescence such as senescence-associated β-galactosidase, senescence-associated heterochromatic foci, and p21, as well as markers of cell kinetics such as Ki-67, was examined in 26 frozen and 82 formalin-fixed liver specimens. Large cell change was frequently detected in chronic hepatitis B cases with advanced histologic staging, particularly those with hepatocellular carcinoma. Senescence-associated β-galactosidase activity,...
Source: Human Pathology - September 7, 2009 Category: Pathology Authors: Hiroko Ikeda, Motoko Sasaki, Yasunori Sato, Kenichi Harada, Yoh Zen, Takeshi Mitsui, Yasuni Nakanuma Tags: Original Contributions Source Type: journals

Primary follicular lymphoma of the extrahepatic bile duct mimicking a hilar cholangiocarcinoma: case report and review of the literatureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a case of a 53-year-old Asian woman who presented with abdominal pain, bloating, dysphagia, and signs of incomplete biliary obstruction, having elevated liver function tests but without increased bilirubin. Imaging studies revealed a mass measuring 6.0 × 8.0 cm at the porta hepatis extending to the right lobe of the liver and obstructing the common hepatic duct, causing mild to moderate intrahepatic biliary dilation and variable occlusion of the right portal vein. At laparotomy, an infiltrative neoplasm was noted at the hilum that involved the common bile duct, right and left hepatic ducts, and the right lobe of...
Source: Human Pathology - August 28, 2009 Category: Pathology Authors: Thalis Christophides, Benjamin Samstein, Jean Emond, Govind Bhagat Tags: Case Studies Source Type: journals

Intravascular polymer material after coil embolization of a giant cerebral aneurysmEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report the case of an 87-year-old female who died after coil embolization of an intracerebral giant aneurysm. Guglielmi detachable (Boston Scientific Neurovascular, Fremont, CA) and Matrix2 coils (Boston Scientific Neurovascular, Fremont, CA) were used during the procedure to occlude the surgically untreatable left supraclinoid carotid artery aneurysm. Postprocedure imaging studies showed scattered areas of acute infarct involving multiple bilateral vascular territories. Autopsy confirmed widespread infarction due to embolized foreign material, morphologically consistent with hydrophilic polymer originating from the coa...
Source: Human Pathology - August 28, 2009 Category: Pathology Authors: Rupal I. Mehta, Rashi I. Mehta, Michael C. Fishbein, Orestes E. Solis, Reza Jahan, Noriko Salamon, Harry V. Vinters, William H. Yong Tags: Case Studies Source Type: journals

SOX2 is highly expressed in squamous cell carcinomas of the gastrointestinal tractEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary: SOX2 is a high-mobility group box embryonic stem cell transcription factor that is expressed in the developing foregut and normal gastric epithelium and is downregulated in intestinal metaplasia of the stomach and esophagus. In addition, SOX2 colocalizes with p63 in the basal layer and plays a critical role in the maintenance of the stratified squamous epithelium of the esophagus. SOX2 expression in squamous cell carcinomas of the gastrointestinal tract has not been previously evaluated. The purpose of this study was to determine whether SOX2 is differentially expressed in squamous cell carcinomas versus adenocarc...
Source: Human Pathology - August 28, 2009 Category: Pathology Authors: Kevin B. Long, Jason L. Hornick Tags: Original Contributions Source Type: journals

Intestinal metaplasia in gallbladder correlates with high amylase levels in bile in patients with a morphologically normal pancreaticobiliary ductEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, intestinal metaplasia in the gallbladder is significantly correlated with high amylase levels in bile in patients with a morphologically normal pancreaticobiliary ductal arrangement. (Source: Human Pathology)
Source: Human Pathology - August 28, 2009 Category: Pathology Authors: Hirotsugu Sakamoto, Hiroyuki Mutoh, Kenichi Ido, Shin Satoh, Machio Kumagai, Hiroko Hayakawa, Kiichi Tamada, Kentaro Sugano Tags: Original Contributions Source Type: journals

Prostate-specific membrane antigen expression in the neovasculature of gastric and colorectal cancersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study provides the first in-depth look at PSMA expression in gastric and colorectal cancer. Because of its highly tumor-restricted expression and its accessibility to targeted therapy, PSMA represents a promising therapeutic and diagnostic target in colorectal and gastric cancer. (Source: Human Pathology)
Source: Human Pathology - August 28, 2009 Category: Pathology Authors: Michael C. Haffner, Irmgard E. Kronberger, Jeffrey S. Ross, Christine E. Sheehan, Matthias Zitt, Gilbert Mühlmann, Dietmar Öfner, Bettina Zelger, Christian Ensinger, Ximing J. Yang, Stephan Geley, Raimund Margreiter, Neil H. Bander Tags: Original Contributions Source Type: journals

Myoepithelial neoplasms involving the vulva and vagina: report of 4 casesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report 4 myoepitheial neoplasms involving the vulva (2 cases) or vagina (2 cases) in patients aged 40 to 45. Two tumors were composed entirely of ovoid or spindle-shaped cells, one entirely of epithelioid cells, and in the other, there was a mixture of spindled and epithelioid cells. Small foci of ductal differentiation with squamous metaplasia were present in one case and a minor stromal component, which varied from myxoid to hyalinized, in all cases. In all cases, the tumor cells were positive for epithelial markers (cytokeratins and/or epithelial membrane antigen) and the myoid markers α smooth muscle actin and calp...
Source: Human Pathology - August 28, 2009 Category: Pathology Authors: Meenakshi Meenakshi, W. Glenn McCluggage Tags: Original Contributions Source Type: journals

Caveolin-1 expression in diffuse gliomas: correlation with the proliferation index, epidermal growth factor receptor, p53, and 1p/19q statusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, the present study indicates that caveolin-1 is not useful as diagnostic marker to differentiate grade II astrocytomas from oligodendrogliomas. (Source: Human Pathology)
Source: Human Pathology - August 28, 2009 Category: Pathology Authors: Valeria Barresi, Francesca Romana Buttarelli, Enrica Vitarelli E, Antonella Arcella, Manila Antonelli, Felice Giangaspero Tags: Original Contributions Source Type: journals

Expression of Grb2 distinguishes classical Hodgkin lymphomas from primary mediastinal B-cell lymphomas and other diffuse large B-cell lymphomasEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary: Growth factor receptor-bound protein 2 is an adaptor molecule that mediates B-cell receptor (BCR) signaling pathways, but the expression of growth factor receptor-bound protein 2 in lymphoma tissues has not been reported. We sought to characterize growth factor receptor-bound protein 2 protein expression in reactive tonsillar tissues and lymphoma tissues obtained from diagnostic biopsies of classical Hodgkin lymphoma, primary mediastinal large B-cell lymphoma, diffuse large B-cell lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, and 20 low-grade B-cell lymphomas. Growth factor receptor-bound protein 2 ex...
Source: Human Pathology - August 28, 2009 Category: Pathology Authors: Rodney R. Miles, Cohra C. Mankey, Charlie E. Seiler, Lauren B. Smith, Julie Teruya-Feldstein, Eric D. Hsi, Kojo S.J. Elenitoba-Johnson, Megan S. Lim Tags: Original Contributions Source Type: journals

Reg IV expression and clinicopathologic features of gallbladder carcinomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we examined Reg IV expression in the gallbladder and gallbladder carcinoma, and measured Reg IV levels in sera from patients with gallbladder carcinoma. Quantitative reverse transcription–polymerase chain reaction revealed that high Reg IV levels were identified in 17 of 31 gallbladder carcinomas, whereas there was no apparent amplification in normal gallbladders. Immunohistochemically, although only a small part of the epithelium with intestinal metaplasia in 2 of 4 cases with adenomyomatosis showed Reg IV expression, Reg IV was negative in all cases with normal gallbladder (n = 15) and cholelithiasis (n ...
Source: Human Pathology - August 28, 2009 Category: Pathology Authors: Hidehiko Tamura, Masayuki Ohtsuka, Mitsutsune Washiro, Fumio Kimura, Hiroaki Shimizu, Hiroyuki Yoshidome, Atsushi Kato, Naohiko Seki, Masaru Miyazaki Tags: Original Contributions Source Type: journals

Growth factor receptor expression in anal squamous lesions: modifications associated with oncogenic human papillomavirus and human immunodeficiency virusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary: High prevalence of squamous anal lesions is linked to oncogenic human papillomavirus (HPV). Human immunodeficiency virus (HIV) promotes anal carcinogenesis. Epidermal growth factor receptor (EGFR), HER2/neu, c-Met, and vascular endothelial growth factor receptor-1 (VEGFR1) (tyrosine kinase growth factor receptors) are implicated in tumor progression, but little is known about their role in anal lesions. We investigated their expression and distribution in normal, dysplastic, and carcinomatous anal epithelium and then tried to analyze the effects on these variables of HPV and the HIV-positive status. Seventy-one HI...
Source: Human Pathology - August 27, 2009 Category: Pathology Authors: Francine Walker, Laurent Abramowitz, Dalila Benabderrahmane, Xavier Duval, Véronique Descatoire, Dominique Hénin, Thérèse Lehy, Thomas Aparicio Tags: Original Contributions Source Type: journals

IMP-3 is differentially expressed in normal and neoplastic lymphoid tissueEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, although IMP-3 expression is seemingly restricted to physiologic germinal center B cells, its expression in lymphomas of germinal center B origin is less robust. However, there does appear to be some association with the latter group of lymphomas, which may prove to have diagnostic or therapeutic relevance as the biologic role of IMP-3 is further elucidated. (Source: Human Pathology)
Source: Human Pathology - August 21, 2009 Category: Pathology Authors: Rebecca L. King, Theresa Pasha, Michele R. Roullet, Paul J. Zhang, Adam Bagg Tags: Original Contributions Source Type: journals

Primary colonic-type adenocarcinoma of the base of the tongue: a previously unreported phenotypeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present, for the first time, 2 primary colonic-type adenocarcinomas of the base of the tongue and discuss their putative origin and the clinicopathologic characteristics. (Source: Human Pathology)
Source: Human Pathology - August 20, 2009 Category: Pathology Authors: Diana Bell, Michael E. Kupferman, Michelle D. Williams, Asif Rashid, Adel K. El-Naggar Tags: Case Studies Source Type: journals

Polycomb group protein Bmi1 is overexpressed and essential in anchorage-independent colony formation, cell proliferation and repression of cellular senescence in cholangiocarcinoma: Tissue and culture studiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the significance of the Bmi1 expression in intrahepatic cholangiocarcinoma. The expression of Bmi1 was examined in intrahepatic cholangiocarcinoma (n = 30; 9 bile ductular carcinoma, 8 intrahepatic cholangiocarcinoma of peripheral type, and 13 of hilar type) by using immunohistochemistry and real-time polymerase chain reaction. The expression level of Bmi1 was assessed in 7 cholangiocarcinoma cell lines. The effect of Bmi1 knockdown was examined in cultured cholangiocarcinoma cells (HuCCT1 and TFK-1) using small interfering RNA. Bmi1 was consistently expressed in nonneoplastic biliary epithelial cells and in al...
Source: Human Pathology - August 20, 2009 Category: Pathology Authors: Motoko Sasaki, Junpei Yamaguchi, Hiroko Ikeda, Keita Itatsu, Yasuni Nakanuma Tags: Original Contributions Source Type: journals

Increased programmed death-1+ tumor-infiltrating lymphocytes in classical Hodgkin lymphoma substantiate reduced overall survivalEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary: Programmed death-1 (PD-1), a protein that is physiologically expressed by germinal center-associated helper T cells, has an inhibitory function on T-cell activity. The distribution of PD-1+ lymphocytes in the microenvironment of Hodgkin lymphoma is not random and can serve as a diagnostic marker. We measured the number of PD-1+ lymphocytes in Hodgkin lymphoma and correlated it with the remaining background lymphocyte populations and known biological and clinical key data on a tissue microarray platform encompassing 280 cases of classical Hodgkin lymphoma and 3 cases of nodular lymphocyte-predominant Hodgkin lympho...
Source: Human Pathology - August 20, 2009 Category: Pathology Authors: Simone Muenst, Sylvia Hoeller, Stephan Dirnhofer, Alexandar Tzankov Tags: Original Contributions Source Type: journals

Down-regulation of ephrin-A5, a gene product of normal cartilage, in chondrosarcomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary: As ephrins have been associated with tumorigenesis and tumor progression, we investigated ephrin-A5 (EFNA5) expression in specimens of normal cartilage and chondrosarcomas of different grade by conventional and quantitative reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry. We detected a significant EFNA5 down-regulation in chondrosarcomas compared with normal cartilage using quantitative RT-PCR (P < .05). The results were confirmed by Western blot and immunohistochemistry. We did not detect any causative genetic or epigenetic alterations in EFNA5 promoter methylation...
Source: Human Pathology - August 20, 2009 Category: Pathology Authors: Thomas Kalinski, Albrecht Röpke, Saadettin Sel, Irina Kouznetsova, Martin Röpke, Albert Roessner Tags: Original Contributions Source Type: journals

Diagnostic biomarkers for renal cell carcinoma: selection using novel bioinformatics systems for microarray data analysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary: The differential diagnosis of clear cell, papillary, and chromophobe renal cell carcinoma is clinically important, because these tumor subtypes are associated with different pathobiology and clinical behavior. For cases in which histopathology is equivocal, immunohistochemistry and quantitative reverse transcriptase–polymerase chain reaction can assist in the differential diagnosis by measuring expression of subtype-specific biomarkers. Several renal tumor biomarkers have been discovered in expression microarray studies. However, due to heterogeneity of gene and protein expression, additional biomarkers are need...
Source: Human Pathology - August 20, 2009 Category: Pathology Authors: Adeboye O. Osunkoya, Qiqin Yin-Goen, John H. Phan, Richard A. Moffitt, Todd H. Stokes, May D. Wang, Andrew N. Young Tags: Original Contributions Source Type: journals

“Primary” leptomeningeal medulloblastomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report the case of an 8-year-old boy who presented with a 2-month history of headaches and mild visual impairment and was found to have a medulloblastoma with primary leptomeningeal involvement. No mass lesion was found on imaging studies, during subsequent intraoperative surgical inspection or at autopsy. The pathologic findings were first documented on cerebrospinal fluid cytologic examination and biopsy of the cerebellum and were later confirmed at necropsy. To our knowledge, this is the third reported case of medulloblastoma identified with primary leptomeningeal involvement without a cerebellar mass and the first s...
Source: Human Pathology - August 19, 2009 Category: Pathology Authors: Rupal I. Mehta, Aaron R. Cutler, Joseph L. Lasky, William H. Yong, Jason T. Lerner, Brandon K. Hirota, Noriko Salamon, Gary W. Mathern, Harry V. Vinters Tags: Case Studies Source Type: journals

Involvement of focal adhesion kinase in the progression and prognosis of gastrointestinal stromal tumorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, Western blot analysis revealed that all 10 of the examined gastrointestinal stromal tumor tissues strongly expressed focal adhesion kinase protein and that phosphorylated focal adhesion kinase was detected in 9 of them. Next, we assessed the expression status of focal adhesion kinase and phosphorylated focal adhesion kinase in 51 cases of gastrointestinal stromal tumor by immunohistochemistry. Positive stainings for focal adhesion kinase and phosphorylated focal adhesion kinase were confirmed in 44 (86.3%) and in 40 cases (78.4%) of the 51 gastrointestinal stromal tumors, respectively. We further found that ...
Source: Human Pathology - August 19, 2009 Category: Pathology Authors: Nobuyuki Kamo, Yoshio Naomoto, Yasuhiro Shirakawa, Tomoki Yamatsuji, Seiichi Hirota, Yasuhiro Fujiwara, Kazuhiro Noma, Kazufumi Sakurama, Munenori Takaoka, Hitoshi Nagatsuka, Mehmet Gunduz, Junji Matsuoka, Noriaki Tanaka Tags: Original Contributions Source Type: journals

Promoter hypermethylation of multiple genes in early gastric adenocarcinoma and precancerous lesionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we determined the methylation frequency of 5 genes, including p16, Runx3, MGMT, DAPK, and RASSF1A, by methylation-specific polymerase chain reaction, in a series of formalin-fixed paraffin-embedded tissues including normal gastric mucosa (n = 20), intestinal metaplasia (n = 14), gastric epithelial dysplasia (n = 27), and early gastric adenocarcinoma (n = 16). Immunohistochemistry was used to determine expression of MGMT and RASSF1A protein. All 20 histologically normal gastric biopsy specimens were methylation-free for all 5 genes. Aberrant hypermethylation of RASSF1A was not detected in any case from intest...
Source: Human Pathology - August 19, 2009 Category: Pathology Authors: Xiao-Ping Zou, Bin Zhang, Xiao-Qi Zhang, Min Chen, Jun Cao, Wen-Jia Liu Tags: Original Contributions Source Type: journals

IMP3 is a novel biomarker for triple negative invasive mammary carcinoma associated with a more aggressive phenotypeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary: IMP3, an oncofetal protein, is a member of the insulin-like growth factor-II (IGF-II) mRNA-binding protein family. Its relevance as a novel biomarker in lung, pancreatic, renal, and cervical adenocarcinoma was recently revealed. However, its role in breast carcinogenesis and tumor progression is not yet established. Basal-like carcinoma was initially identified by gene expression profiling. It accounts for 15% to 30% of all breast cancers. These tumors express basal epithelial markers including cytokeratin 5 but lack expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor rece...
Source: Human Pathology - August 19, 2009 Category: Pathology Authors: Otto Walter, Manju Prasad, Shaolei Lu, Robert M. Quinlan, Kathryn L. Edmiston, Ashraf Khan Tags: Original Contributions Source Type: journals

Prognostic relevance of CCN3 in Ewing sarcomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we assessed the prognostic value of CCN3 (Nov), a matricellular protein that play crucial roles in bone formation. Polyclonal antibodies directed against each of the different CCN3 modules were used to identify variant CCN3 proteins in tumors and to draw potential relationships between the expression of these variants and the outcome of patients with Ewing sarcoma. Our results confirmed that expression of the full-length CCN3 in Ewing sarcoma is associated to a worse prognostic. Furthermore, we report a possible relationship between the expression of a CCN3 protein lacking an internal module (von Willebrand ...
Source: Human Pathology - August 19, 2009 Category: Pathology Authors: Bernard Perbal, Noureddine Lazar, Diana Zambelli, Jose Antonio Lopez-Guerrero, Antonio Llombart-Bosch, Katia Scotlandi, Piero Picci Tags: Original Contributions Source Type: journals

Direct contribution of epithelium to organ fibrosis: epithelial-mesenchymal transitionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary: Fibrosis of epithelial parenchymal organs and end-stage organ failure represent the final common pathway of many chronic diseases and are a major determinant of morbidity and mortality worldwide. Fibrosis is a complex response initiated to protect the host from an injurious event; nevertheless, it leads to serious organ damage when it becomes independent from the initiating stimulus. It involves massive deposition of matrix by an expanded pool of fibrogenic cells, disruption of the normal tissue architecture, and parenchymal destruction. Fibroblasts, the effector cells of matrix production, when engaged in fibroge...
Source: Human Pathology - August 19, 2009 Category: Pathology Authors: Marcello Guarino, Antonella Tosoni, Manuela Nebuloni Tags: Progress in Pathology Source Type: journals

Authors' reply to letter to the editor: direct evidence of mutational inactivation of SMARCB1/INI1 in epithelioid sarcomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We appreciate the interest of Flucke et al in our recent article on “Infrequent SMARCB1/INI1 gene alteration in epithelioid sarcoma: a useful tool in distinguishing epithelioid sarcoma from malignant rhabdoid tumor” . Their recent investigation revealed infrequent SMARCB1/INI1 (INI1) gene alterations (7.7%) in epithelioid sarcoma (ES) with loss of INI1 expression, whereas Modena et al demonstrated frequent INI1 gene alterations (83.3%) in 6 cases of ES with loss of INI1 expression. We also reported infrequent INI1 gene alterations (10.3%) in 39 cases of ES with loss of INI1 expression . (Source: Human Pathology)
Source: Human Pathology - August 18, 2009 Category: Pathology Authors: Kenichi Kohashi, Yoshinao Oda, Masazumi Tsuneyoshi Tags: Correspondence Source Type: journals

Re: Infrequent SMARCB1/INI1 gene alteration in epithelioid sarcoma: a useful tool in distinguishing epithelioid sarcoma from malignant rhabdoid tumor: Direct evidence of mutational inactivation of SMARCB1/INI1 in epithelioid sarcomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We read with great interest the recent article by Kohashi et al in which they report infrequent SMARCB1/INI1 gene alteration in epithelioid sarcomas (ES). They investigated 16 proximal-type and 23 distal-type ES with loss of INI1 expression but detected INI1 gene alterations in only 4 proximal-type tumors, that is, 2 homozygous deletions affecting the whole gene and 2 intragenic deletions of 1 (in exon 9) and 2 (in exon 3) base pairs. In an earlier study , homozygous INI1 gene deletions, but no mutations, were reported in 5/5 proximal type ES and in 0/1 distal type ES, all with loss of INI1 expression. However, in the case...
Source: Human Pathology - August 18, 2009 Category: Pathology Authors: Uta Flucke, Pieter J. Slootweg, Thomas Mentzel, Patrick Pauwels, Theo J.M. Hulsebos Tags: Correspondence Source Type: journals