O-140 Uterine septum: clinical implications on fertility and obstterics outcomes. A systematic review and meta-analysis
AbstractStudy questionHow does the septate uterus and his metroplasty influence pregnancy rate (PR), live birth rate (LBR), spontaneous abortion rates (SA) and preterm labour rates (PL)?Summary answerUterine septum is associated with a poor reproductive outcome. Metroplasty reduce the rate of SA but non-conclusive evidence can be extrapolated about PR and PL.What is known alreadyDifferent studies evaluated the correlation between uterine septum and reproductive outcomes. On one hand, studies reported its association with poor obstetrics outcomes. On the other hand, recent studies raised doubts about the effectiveness of se...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-139 Complete removal of uterine septum tissue as a wedge, an alternative method for patients suffering from recurrent pregnancy loss or infertility, a retrospective cohort study
AbstractStudy questionIs hysteroscopic wedge septectomy (HWS) an effective and safe method for reducing the risk of miscarriage and improving the reproductive outcome in patients with recurrent pregnancy loss or infertility history?Summary answerHWS is a safe and effective method for RPL and infertility cases with statistically significant improvement in pregnancy chances and reproductive outcomes.What is known alreadyWith regard to the persisting uncertainty around the effectiveness of septum resection in recurrent miscarriage and infertility cases, there may be alternative methods to better address the pathophysiology of...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-042 Assisted Reproductive Technology (ART) in Europe 2018 and development of a strategy of vigilance. preliminary results generated from european registers by ESHRE
AbstractStudy questionWhat are the reported data on cycles in ART, IUI and fertility preservation interventions in 2018 as compared to previous years, as well as the main trends over the years?Summary answerAUTHOR: The 22th ESHRE report on ART and IUI shows a progressive increase in reported treatment cycle numbers in Europe, a small decrease in the number of transfers (IVF + ICSI) with more than one embryo with a trend to decreasing multiple delivery rates, higher pregnancy and delivery rates after FER compared to fresh IVF and ICSI cycles, and outcomes for IUI cycles similar to previous years.What is known alreadySince 1...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-041 Functional sperm diagnostics: The evidence behind commercially available tests
Abstract textDespite the fact that the semen analysis has remained the cornerstone of the male infertility evaluation for decades, it has significant limitations. Among them are the inability to completely predict reproductive potential and the inability to analyze the fitness of individual sperm. There remains a real need for novel tests that can determine which interventions for male infertility are the most likely to result in a significant improvement in reproductive potential, to analyze the chance of success or failure with ART and to determine if there exists a sub population of sperm whose use could lead to optimal...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-025 Value of hysteroscopy
Abstract textThe 3 main characters in any ART are the uterus, the endometrium, and the embryo. With the rutinary use of 2D US and especially 3D US in infertile patients we are able to assess most of the uterine anomalies and intrauterine pathologies.Until recently, the assessment of the endometrial cavity with hysteroscopy was reserved only for cases of IVF failure, as a complementary evaluation to ensure that nothing was missed during the ultrasound scan.We also need to remember how hysteroscopy was performed in the past and, unfortunately, in some cases even today. In the old days, to assess the endometrial cavity, it wa...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-024 Endoscopic management of the unexplained infertility, what does it add?
ConclusionThe inappropriate use of “unexplained infertility” by omitting the diagnostic endoscopy in the exploration of the infertile patient, can hide undiagnosed and treatable pathology, jeopardizing possibilities for patients for a spontaneous conception and can be responsible for reduced pregnancy rates after IVF. (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P-479 Are FET and IUI cycles less emotionally difficult for patients than IVF? Evidence from smartphone app based emotional tracking data
AbstractStudy questionIs the emotional experience different in FET and stimulated IUI cycles compared to IVF cycles?Summary answerEmotional tracking data demonstrated cautious optimism and lower harm emotions in IUI, but FET cycles are associated with higher harm emotions than fresh IVF.What is known alreadyIt is sometimes claimed on clinic websites and by advocates for elective freeze all that FET cycles are inherently less stressful. However, little research evaluates the emotional difference between fresh and frozen cycles and the assumed emotional ease of FET may reflect clinician interpretation/bias rather than patien...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P-556 Pre-selected for an award: NAT10-mediated N4-acetylcytidine in RNA regulates mouse oocyte maturation in vitro
This study was performed in vitro. The role of NAT10-mediated ac4C in vivo remains to be elucidated. Also, limited by current techniques, ac4C modification in oocytes cannot be detected. Our exploration of regulated genes and ac4C binding proteins were performed in somatic cell lines.Wider implications of the findingsPost-transcriptional modulation is crucial in oocyte maturation. Our study using in-vitro systems for mouse oocyte identified NAT10-mediated ac4C as an important regulator in IVM. It provided a new insight into the epigenetic mechanisms of IVM, which may lead to improvement of clinical IVM systems.Trial regist...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P-736 Elevated levels of ambient air pollutants increase the primary sex ratio in human embryos
AbstractStudy questionAre there any associations between ambient outdoor air pollution and the primary sex ratio (PSR)?Summary answerShort-term exposure to increased PM10, PM2.5 and NO2 levels were significantly associated with higher PSR.What is known alreadyPSR estimates represent a backward extrapolation from data based on spontaneous or induced abortions, fetal deaths or live births and are usually male-biased. A recent study, analyzing 3- to 6-day-old embryos derived from assisted reproductive technology (ART) procedures, showed that the sex ratio at conception is unbiased (0.5). Epidemiologic studies of air pollution...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-086 End-to-end deep learning for recognition of ploidy status using time-lapse videos
AbstractStudy questionOur Retrospective study is to investigate an end-to-end deep learning model in identifying ploidy status through raw time-lapse video.Summary answerOur deep learning model demonstrates a proof of concept and potential in recognizing the ploidy status.What is known alreadySince the time-lapse system has been introduced into the IVF lab, the relationship between morphogenetic and ploidy status has been often discussed. However, the result has not yet reached a united conclusion due to some limitations such as human labeling. Besides the statistical approach, deep learning models have been utilized for p...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-080 Activated AKT/mTOR signalling in peripheral blood of women with premature ovarian insufficiency and its correlation with variable FMR1 expression profiles
AbstractStudy questionHow predictive are gene expression levels of AKT/mTOR-signalling-pathway genes in peripheral blood of patients with premature ovary insufficiency (POI) and is there a link toFMR1-expression?Summary answerAKT1, TSC2, mTOR, S6K andFOXO3-expression-levels are significantly upregulated in POI-patients and demonstrate a positive correlation withFMR1-expression-level in case ofmTOR-, S6K andFOXO3.What is known alreadyThe AKT/mTOR-signalling-pathway is involved in a range of cellular functions. In female germline it regulates early follicular-activation and follicular-pool-maintenance. Over the past few year...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –192 Efficacy of postponement of intracytoplasmic sperm injection timing after spindle visualization for Metaphase I oocytes
This study was approved by the institutional review board.Participants/materials, setting, methodsAs a control group, 464 MetaphaseIIoocytes that underwent ICSI immediately after visualization of the spindle were used. In group A, 103 MI oocytes underwent ICSI immediately after the first polar body release and spindle visualization, and in group B, 105 oocytes underwent ICSI 2–3 hours after spindle visualization. The primary outcomes were fertilization rates, degeneration, cleavage, embryo blastocyst formation, and utility rates. Outcomes were compared among the three groups.Main results and the role of chanceThe ba...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –191 Time-lapse videography reveals morphometric and morphokinetic differences in the pronuclei of male and female human zygotes
AbstractStudy questionDo morphometric and morphokinetic profiles of pronuclei (PN) following intracytoplasmic sperm injection (ICSI) vary between male and female human zygotes?Summary answerMale and female zygotes displayed different PN morphometrics and morphokinetics. Additionally, variations were identified between sperm-originated (SPN) and oocyte-originated (OPN) pronuclei.What is known alreadyPrevious studies have investigated the use of PN-associated parameters via static observations as indicators of zygote viability, including size equality or juxtaposition. However, recent clinical application of time-lapse video...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –190 Can pregnancy be predicted according to mean infra-red attenuated total reflectance (MIR-ATR) spectrometry of incubation medium of embryos in IVF?: A proof of concept
AbstractStudy questionCan MIR-ATR spectrometry of embryo incubation medium be used to predict embryo quality and IVF treatment results.Summary answerMIR-ATR spectrometry is able to distinguish between good and poor embryo quality and may improve the prediction of pregnancy based on better embryo selection.What is known alreadyInfra Red (IR) spectra enable to determine if certain chemical functional components are present in a molecule. Attenuated total reflection (ATR) spectroscopy is a well established, simple and rapid technique for objective classification of biological samples. ATR spectrometry was investigated and con...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –085 Sperm retrieval after microdissection testicular sperm extraction (micro-TESE) and pregnancy outcomes of intracytoplasmic sperm injection (ICSI) in men with Klinefelter syndrome
AbstractStudy questionWhat are the sperm retrieval rate and ICSI outcomes in azoospermic men with Klinefelter syndrome (KS)?Summary answerIn men with KS, a sperm retrieval rate of 51.3% after the first attempt micro-TESE, and 4 live births after ICSI were observed. What is known already: Klinefelter syndrome (KS) is encountered in 10% of men with azoospermia. Micro-TESE is presently used to treat infertility for KS patients with nonobstructive azoospermia. The retrieved sperms can be used for ICSI. Study design, size, duration: From June 2019 to July 2020, 39 azoospermic patients with KS were examined for the presence of t...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
