Does Xpert Carba R assay detect carbapenemase-producing organism in Gram-negative sepsis in neonates ?
R Usha Devi, Thangaraj Abiramalatha, Ashok Chandrasekaran, Shaik Mohammad Shafi Jan, Prakash Amboiram, Umamaheswari BalakrishnanIndian Journal of Medical Microbiology 2020 38(3):500-501 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: R Usha Devi Thangaraj Abiramalatha Ashok Chandrasekaran Shaik Mohammad Shafi Jan Prakash Amboiram Umamaheswari Balakrishnan Source Type: research

Burkholderia vietnamiensis causing a non-lactational breast abscess in a non-cystic fibrosis patient in Tamil Nadu, India
We present here a report of a case of Burkholderia vietnamiensis causing a non-lactational breast abscess in a non-CF patient. The pathogen was identified as B. cepacia using Vitek system and matrix-assisted laser desorption ionisation–time of flight. This was confirmed by polymerase chain reaction (PCR) using recA genus-specific gene and sequencing of the PCR amplicons. recA-restriction fragment length polymorphism and recA gene sequencing revealed that the isolate is B. vietnamiensis. This is the first description of B. vietnamiensis isolated from a clinical case from India. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Anusha Rohit M Shraddha Rani N Suresh Anand Cynthia Chellappa P Mohanapriya Indrani Karunasagar Iddya Karunasagar Vijaya Kumar Deekshit Source Type: research

Aspergillus mediastinitis in a post-operative immunocompetent child
We describe the case of an immunocompetent 2-month-old child with obstructed supracardiac total anomalous pulmonary venous circulation (TAPVC) and severe pulmonary artery hypertension, who underwent TAPVC repair through median sternotomy and developed post-operative mediastinitis due to Aspergillus flavus. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Anand Kumar Mishra Vidur Bansal Gradlin Roy Vikram Halder Parakritii Gupta Arunaloke Chakrabarti Source Type: research

First report of Vibrio cholerae O9, novel st520, isolated from a child with bacteraemia-associated sepsis
We describe a case of sepsis in a child with spinal dysraphism caused by a V. cholerae O9 strain belonging to a novel sequence type (ST520). The present case highlights the need of considering V. cholerae non-O1/O139 as one of the pathogens while dealing with sepsis cases, and also, the study expounds the importance of proper characterisation of the pathogen for an effective treatment. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Nandita Shashindran Lekshmi Narendrakumar Suhas Udayakumaran Divya Mavanavila Vijayakumar Sabu Thomas Anil Kumar Source Type: research

Adrenal histoplasmosis in immunocompetent individuals a case series from the North-Western part of India, Rajasthan province: An emerging endemic focus
We describe five immunocompetent men with adrenal histoplasmosis presenting with constitutional symptoms. Four patients had bilateral adrenal involvement, whereas one had unilateral adrenal mass. Three patients had adrenal insufficiency at presentation and the other two developed adrenal insufficiencies during follow-up. All the patients received amphotericin B and itraconazole treatment which led to symptomatic improvement but adrenal insufficiency persisted in all patients at the end of the follow-up. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Balram Sharma Hardeva Ram Nehara Vijay Kumar Bhavi Pratibha Maan Sanjay Saran Source Type: research

Streptococcus dysgalactiae septic arthritis of sternoclavicular joint with bacteraemia
Pruthu Narendra Dhekane, Ram Gopalakrishnan, V Lakshmi Sree, V Dedeepiya DevaprasadIndian Journal of Medical Microbiology 2020 38(3):481-484 Invasive infections due to Streptococcus dysgalactiae are uncommon in the adult population, and sternoclavicular joint septic arthritis (SCSA) is usually caused by other organisms such as Staphylococcus aureus and Pseudomonas aeruginosa. We hereby report a case of SCSA caused by this organism. The patient responded well to intravenous antibiotics and recovered fully without any surgical intervention. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Pruthu Narendra Dhekane Ram Gopalakrishnan V Lakshmi Sree V Dedeepiya Devaprasad Source Type: research

An unexpected host in a soft-tissue lesion of thigh
We describe the case of a 35-year-old female patient who presented with a slow-growing soft-tissue swelling with ulceration over the thigh. On clinical and radiological examination, a provisional diagnosis of soft-tissue neoplasm was made. After resection, histopathological sections showed a closely packed cyst with innumerable endospores. The present case report documents the rare occurrence of an incidentally detected cutaneous rhinosporidiosis causing diagnostic difficulty. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Kavita Jain SM Sarfaraj Moumita Sengupta Chhanda Datta Uttara Chatterjee Source Type: research

A rare case of Colletotrichum truncatum keratitis in a young boy with complete healing after medical treatment
Josephine S Christy, A Balraj, Aditi AgarwalIndian Journal of Medical Microbiology 2020 38(3):475-477 Colletotrichum is a rare fungal pathogen, which is known to cause anthracnose in plants and keratitis or subcutaneous infections in humans. Among the seven Colletotrichum species reported in eye infections, truncatum species is usually virulent with poor visual prognosis even after surgical treatment. Here we report a case of Colletotrichum truncatum keratitis in a young boy with thorn injury that completely resolved with topical natamycin and voriconazole. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Josephine S Christy A Balraj Aditi Agarwal Source Type: research

Mycotic keratitis due to Cylindrocarpon lichenicola: Successful salvage of the eye
M Suchitra Shenoy, Rajesh R Nayak, Vijay Pai, K Archana BhatIndian Journal of Medical Microbiology 2020 38(3):472-474 We hereby report a successfully salvaged eye due to mycotic keratitis by Cylindrocarpon lichenicola in a 60-year-old female from Kasaragod (Kerala). The patient came with a history of pain, photophobia and decreased vision of the right eye. The microbiological investigations of the corneal scraping revealed C. lichenicola. C. lichenicola is a soil saprophyte. Since the ulcer worsened paracentesis followed by therapeutic keratoplasty and adjunct therapy with natamycin drops, voriconazole drops and oral keto...
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: M Suchitra Shenoy Rajesh R Nayak Vijay Pai K Archana Bhat Source Type: research

False-positive blood cultures: The need for follow-up
Suverna Kirolikar, Ambreen Pandrowala, Sangeeta Joshi, Ruchira Misra, Sujata MushrifIndian Journal of Medical Microbiology 2020 38(3):469-471 The diagnosis of blood-borne infections in immunocompromised patients is a major challenge for the clinical microbiology laboratory. Isolation of bloodborne pathogens in these patients has profound clinical implications, yet is fraught with technical problems, including the presence of unusual and difficult to isolate pathogens. Coupled with this is the problem of false-positive blood culture signals from automated blood culture systems which further delays the definitive diagnosis....
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Suverna Kirolikar Ambreen Pandrowala Sangeeta Joshi Ruchira Misra Sujata Mushrif Source Type: research

A real-time polymerase chain reaction-based approach for qualitative estimation of viral RNA in organ tissues of coxsackievirus A-16-infected neonatal mice
Sanjaykumar Sidram Tikute, Shailesh Dattatraya Pawar, Samruddhi Satish Kawale, Deepa Kailash Sharma, Varanasi GopalkrishnaIndian Journal of Medical Microbiology 2020 38(3):464-468 Hand, foot and mouth disease (HFMD) is a paediatric disease associated with enteroviruses (EVs). Among EVs, coxsackievirus A-16 (CVA-16) strain is currently in circulation and causing outbreaks in India. Neonatal mice (Institute of Cancer Research) strains were infected with CVA-16 strain isolated from HFMD patients to conduct pathological and molecular studies. Infected organs were harvested as per time points. A real-time polymerase chain reac...
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Sanjaykumar Sidram Tikute Shailesh Dattatraya Pawar Samruddhi Satish Kawale Deepa Kailash Sharma Varanasi Gopalkrishna Source Type: research

Prevalence of COVID-19 antibodies in healthcare workers at the peak of the pandemic in Mumbai, India: A preliminary study
Tanu Singhal, Sweta Shah, Reshma Naik, Amreen Kazi, Pooja ThakkarIndian Journal of Medical Microbiology 2020 38(3):461-463 Healthcare worker (HCW) infections due to COVID-19 are of serious consequence. Testing for antibodies against COVID-19 in HCWs has been previously recommended. We conducted a serosurvey in HCWs at a private hospital in Mumbai which is treating COVID patients. A total of 244 HCWs were tested. The prevalence of infection in asymptomatic HCWs was 4.3% and in previously symptomatic untested HCWs was 70%. We recommend that HCWs with a previous history of COVID symptoms who were not te...
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Tanu Singhal Sweta Shah Reshma Naik Amreen Kazi Pooja Thakkar Source Type: research

Arterial blood gas as a prognostic indicator in patients with sepsis
Sayan Mukherjee, Suvrajyoti Das, Sudipta Mukherjee, Pralay Shankar Ghosh, Sanjay BhattacharyaIndian Journal of Medical Microbiology 2020 38(3):457-460 Abnormal arterial blood gas (ABG) among patients with sepsis is an important prognostic indicator. All-cause mortality was the highest among patients with respiratory acidosis (4/9 = 44.4%), followed by those having metabolic acidosis (3/8 = 37.5%). Median length of hospital and intensive care unit stay was 15.75 days and 6.25 days for those with abnormal ABG and 11 and 3.5 days among those with normal ABG. Median health-care expe...
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Sayan Mukherjee Suvrajyoti Das Sudipta Mukherjee Pralay Shankar Ghosh Sanjay Bhattacharya Source Type: research

Analysis of multi-sample pools in the detection of SARS-CoV-2 RNA for mass screening: An Indian perspective
Sangeeta Deka, Deepjyoti Kalita, Amit Mangla, Ravi ShankarIndian Journal of Medical Microbiology 2020 38(3):451-456 In the current COVID-19 crisis, many national healthcare systems are confronted with a huge demand for mass testing and an acute shortage of diagnostic resources. Considering group testing as a viable solution, this pilot study was carried out to find the maximum number of samples that can be pooled together to accurately detect one positive sample carrying the severe acute respiratory syndrome-coronavirus 2 viral RNA from different pools. We made different pool sizes ranging from 5 to 30 samples. Three posi...
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Sangeeta Deka Deepjyoti Kalita Amit Mangla Ravi Shankar Source Type: research

E-tutorials to accentuate & #8211; Clinical microbiology learning
Sheetal U HarakuniIndian Journal of Medical Microbiology 2020 38(3):448-450 Competency-based medical education has challenged the educators to adopt new teaching modalities to instil self-directed learning among children. E-learning in the form of blended learning is studied to facilitate clinical microbiology learning. A class of 200 students was introduced to the process of Google Classroom. Two topics were discussed in online classrooms. The students attempted online tests on the topics, that were later discussed in small groups in offline class. Students found that analytical/interpretive questions accentuated learnin...
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Sheetal U Harakuni Source Type: research