International Journal of Laboratory HematologyThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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253 records returned

Age-related plasma reference ranges for two heparin-binding proteins--vitronectin and platelet factor 4.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was conducted to establish age-related reference ranges for two heparin-binding proteins--vitronectin and platelet factor 4 (PF4)--and to determine if the quantitative values of these proteins may contribute to the reported age-dependent effect of unfractionated heparin (UFH). Plasma samples were obtained from healthy children aged between 1 month and 16 years and from healthy adult volunteers. Two commercial kits were used to measure plasma vitronectin and PF4 levels. Results were reported as mean and boundaries including 95% of the population. Plasma vitronectin levels for children aged 1-5 years were signific...
Source: International Journal of Laboratory Hematology - November 19, 2009 Category: Hematology Authors: Newall F, Johnston L, Ignjatovic V, Summerhayes R, Monagle P Tags: Int J Lab Hematol Source Type: journals

Evaluation of mean platelet volume in the differential diagnosis of thrombocytopenia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19909383 [PubMed - in process] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - November 19, 2009 Category: Hematology Authors: Ntaios G, Papadopoulos A, Chatzinikolaou A, Girtovitis F, Kaiafa G, Savopoulos C, Hatzitolios A Tags: Int J Lab Hematol Source Type: journals

The utility of the Sysmex XE-2100 analyzer's NEUT-X and NEUT-Y parameters for detecting neutrophil dysplasia in myelodysplastic syndromes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary The diagnosis of myelodysplastic syndromes (MDS) is based on morphological changes in the blood and bone marrow. The parameters NEUT-X and NEUT-Y of the Sysmex XE-2100 analyzer could help detect neutrophil dysplasia. A control group of 50 patients, along with 50 postpartum patients, 50 anemias, 50 leukopenias, 50 patients with microscopically visible hypergranulated neutrophils and 50 MDS patients were assessed. The NEUT-X and NEUT-Y values (mean +/- SD) for the control group were 1346 +/- 28.2 and 420 +/- 19.3, respectively, with the anemia and leukopenia groups giving similar values. The postpartum and hyperg...
Source: International Journal of Laboratory Hematology - November 10, 2009 Category: Hematology Authors: Furundarena JR, Araiz M, Uranga M, Sainz MR, Agirre A, Trassorras M, Uresandi N, Montes MC, Argoitia N Tags: Int J Lab Hematol Source Type: journals

Audit on the use of coagulation screen in the acute admission unit in a tertiary centre.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19888993 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - November 2, 2009 Category: Hematology Authors: Wakil A, Ng JM, Medlock R, Masson E Tags: Int J Lab Hematol Source Type: journals

Hirudin-induced pseudothrombocytopenia in a patient with EDTA-dependent platelet aggregation: report of a new laboratory artefact.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19878358 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - October 28, 2009 Category: Hematology Authors: Robier C, Neubauer M, Sternad H, Rainer F Tags: Int J Lab Hematol Source Type: journals

Could rapid particle gel immunoassay (ID-PaGIA) replace standard ELISA for laboratory detection of heparin/pf4 antibodies?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19878359 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - October 28, 2009 Category: Hematology Authors: Antovic JP, Norberg EM, Sten-Linder M Tags: Int J Lab Hematol Source Type: journals

Direct international normalized ratio determination using multicalibrators is more responsive than the conventional method for measuring prothrombin time.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary Direct international normalized ratio (INR) determination using certified INR plasmas was shown to improve precision and accuracy. We evaluated the utility of a multicalibrator in determining INR. INR values were measured in 493 blood samples from patients subjected to anticoagulation therapy (320) and control subjects (173). Study was performed using CA-7000 coagulation analyzer (Sysmex, Japan) with Thromborel S (Dade Behring, Germany). Direct INR values were obtained using PT-Multi Calibrator (Dade Behring) composed of five lyophilized calibrant plasmas. Conventional INR values were calculated from mean norma...
Source: International Journal of Laboratory Hematology - October 28, 2009 Category: Hematology Authors: Cha CH, Park CJ, Kim DH, Kim MJ, Cho YU, Jang S, Chi HS Tags: Int J Lab Hematol Source Type: journals

Plasma thrombopoietin level and platelet indices in hemodialysis patients receiving recombinant human erythropoietin.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study included 25 HD patients undergoing treatment with rHuEPO at 9000 IU/week. These patients were divided into two groups by reference PLT of 130 x 10(9)/l [eight patients with low PLT (L-PLT group) and 17 patients with normal PLT (N-PLT group)], and TPO level and platelet indices in each group were compared with those in nine HD patients not receiving rHuEPO. In HD patients, the mean value of MPV was slightly higher and the mean values of PLT, PCT, and MPC were significantly lower than those in healthy controls. TPO levels were significantly higher in patients receiving rHuEPO than in patients not receiving rHuEPO....
Source: International Journal of Laboratory Hematology - October 28, 2009 Category: Hematology Authors: Asanuma M, Seino K, Mizuno T, Nasu M, Yamauchi F, Fujishima M Tags: Int J Lab Hematol Source Type: journals

Expression patterns of specific promyelocytic/retinoic acid receptor-alpha transcripts in patients with acute promyelocytic leukemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary Several additional promyelocytic/retinoic acid receptor-alpha (PML/RARalpha) transcripts besides bcr1, bcr2, and bcr3 have been identified in patients with acute promyelocytic leukemia (APL). However, the expression levels of these specific isoforms and their clinical relevance have not been studied to date. The real-time quantitative polymerase chain reaction was established to detect each specific isoform of PML/RARalpha transcripts (bcr1/2, P46R3, P4R3, bcr3, and P2R3) in 46 APL patients. Whereas P46R3 and P4R3 isoforms were concurrently expressed in both bcr1- and bcr2-positive patients, P2R3 isoform was ex...
Source: International Journal of Laboratory Hematology - October 26, 2009 Category: Hematology Authors: Lin J, Han LX, Qian J, Wang YL, Yao DM, Qian Z, Yang XF, Sheng XJ Tags: Int J Lab Hematol Source Type: journals

International Council for Standardization in Haematology technical report 1-2009: new reference material for haemiglobincyanide for use in standardization of blood haemoglobin measurements.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary The International Council for Standardization in Haematology (ICSH) in conjunction with Eurotrol, B.V. has released a new lot of the haemiglobincyanide or haemoglobin standard. This technical report describes the purpose, methodology in manufacturing, summary of value assignment data and availability of this standard material used for the standardization and calibration of whole blood haemoglobin measurements on most haemoglobinometers and automated blood cell counters throughout the world. PMID: 19863683 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - October 26, 2009 Category: Hematology Authors: Davis BH, Jungerius B, Tags: Int J Lab Hematol Source Type: journals

Utility of hematologic and volume, conductivity, and scatter parameters from umbilical cord blood in predicting chorioamnionitis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary We evaluated the utility of routine hematologic parameters and volume, conductivity, and scatter (VCS) parameters from umbilical cord blood in predicting pathologically confirmed chorioamnionitis. Chorioamnionitis is strongly associated with early neonatal sepsis and is the major in utero source of infectious exposure. We prospectively identified mothers who had placenta submitted for pathology and subsequently identified corresponding neonate cord blood sent for routine blood typing (n = 99). Cord blood was then sent for routine complete blood count with differential (CBCD). Among the same neonates, we retrosp...
Source: International Journal of Laboratory Hematology - September 27, 2009 Category: Hematology Authors: Lee JC, Ahern TP, Chaves FP, Quillen K Tags: Int J Lab Hematol Source Type: journals

DNA Index in childhood acute lymphoblastic leukaemia: a karyotypic method to validate the flow cytometric measurement.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary The DNA index (DI) is a prognostic factor in childhood acute lymphoblastic leukemia (ALL). The accuracy of DI measurement is important for treatment stratification: hyperdiploidy with DI >/= 1.16 is predictive of favorable prognosis whereas hypodiploidy is associated with poor prognosis. The aim of this study was to validate the accuracy of the DI measured by flow cytometry (FCM) by comparison with the karyotype. From samples of 112 childhood ALL, we created a formula to calculate a theoretical DNA index (tDI) based on the blast cell karyotype, taking into account the additional or missing chromosome materia...
Source: International Journal of Laboratory Hematology - September 27, 2009 Category: Hematology Authors: Rachieru-Sourisseau P, Baranger L, Dastugue N, Robert A, Geneviève F, Kuhlein E, Chassevent A Tags: Int J Lab Hematol Source Type: journals

An evaluation of the SPIFE(R) 3000 semi-automated gel electrophoresis system for the identification of hemoglobin variants and comparison of relative electrophoretic mobilities with manual gel electrophoresis methods.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary Laboratory identification of hemoglobin (Hb) variants can involve multiple techniques. The use of semi-automated instruments that perform gel electrophoresis and staining, such as the SPIFE((R)) 3000 electrophoresis system, can greatly reduce the labor required for these commonly used techniques. We performed a comparison of the method involved in SPIFE((R)) 3000 system with those of manual gel electrophoresis. A total of 22 540 samples were analyzed using the SPIFE((R)) 3000, and compared with mobilities on cellulose acetate and citrate agar gels using standard manual methods. The results were compared using r...
Source: International Journal of Laboratory Hematology - September 16, 2009 Category: Hematology Authors: Hoyer JD, Markley KM, Savedra ME, Kubik KS, Scheidt RM Tags: Int J Lab Hematol Source Type: journals

Sideroblastic changes of the bone marrow can be predicted by the erythrogram of peripheral blood.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we analyzed the value of a specific erythrogram pattern from peripheral blood, produced by the ADVIA((R))120 cell counter, to predict sideroblastic changes in the bone marrow. In a two step-design study, we first showed that 32/38 consecutive patients reporting >/=15% RS had such a pattern in the erythrogram. In the second step, we prospectively identified over a period of 32 months 21 patients with this typical erythrogram; 20/21 had >/=15% RS in the bone marrow. Hence, by this validation, we confirm that the erythrogram is highly predictive of RS in the bone marrow. The interpretation of the erythrog...
Source: International Journal of Laboratory Hematology - August 24, 2009 Category: Hematology Authors: Rovó A, Stüssi G, Meyer-Monard S, Favre G, Tsakiris D, Heim D, Halter J, Arber C, Passweg J, Gratwohl A, Tichelli A Tags: Int J Lab Hematol Source Type: journals

Relationships of serum free thyroxine and erythrocyte measures in euthyroid HFE C282Y homozygotes and control subjects: the HEIRS Study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary Hemoglobin (Hb) levels and mean corpuscular volume (MCV) are abnormal in some persons with hemochromatosis or thyroid disorders. We sought to determine whether serum free thyroxine (T4) affects erythrocyte measures in euthyroid adults with or without C282Y homozygosity. We evaluated 488 white HFE C282Y homozygotes and controls (no HFE C282Y or H63D; normal serum iron measures) identified in screening; we excluded those with thyroid disorders, anemia, erythrocytosis, or serum ferritin (SF) <34 pmol/l. In the remaining 141 C282Y homozygotes and 243 controls, we evaluated correlations of log(10) free T4 with H...
Source: International Journal of Laboratory Hematology - August 24, 2009 Category: Hematology Authors: Barton JC, Leiendecker-Foster C, Reboussin DM, Adams PC, Acton RT, Eckfeldt JH, Tags: Int J Lab Hematol Source Type: journals

Detection of circulating antierythropoietin antibodies in patients with end stage renal disease on regular hemodialysis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary Recombinant human erythropoietin (rHuEPO) has been successfully and safely used to treat anemia in patients with end stage renal disease (ESRD). The safety profile of rHuEPO had been considered to be excellent with possible exception of hypertension and increased risk of dialysis access thrombosis. Recently, antibody-mediated pure red cell aplasia associated with administration of rHuEPO has been identified as a cause of major concern; we aimed to detect and evaluate the presence of anti-EPO antibodies in patients with ESRD on regular dialysis who are using rHuEPO. Serum anti-EPO antibodies were detected by enz...
Source: International Journal of Laboratory Hematology - August 22, 2009 Category: Hematology Authors: El-Din MM, Attia FM, Labib SM, Omar W Tags: Int J Lab Hematol Source Type: journals

Immature platelet fraction measurement in patients with chronic liver disease: a convenient marker for evaluating cirrhotic change.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined whether the measurement of the immature platelet fraction (IPF) in thrombocytopenic patients with liver dysfunction is useful as a rapid and noninvasive method for the differential diagnosis of chronic liver diseases. We examined 20 liver cirrhosis patients, 56 patients with chronic hepatitis, 9 patients with fatty liver, and 86 patients without liver disease. The percentage value of IPF (IPF%) was measured using an XE-2100 multiparameter automatic hematology analyzer. Using a receiver operating characteristic curve, we found diagnostic significance of the absolute platelet count and the absolute number of the ...
Source: International Journal of Laboratory Hematology - August 18, 2009 Category: Hematology Authors: Nomura T, Kubota Y, Kitanaka A, Kurokouchi K, Inage T, Saigo K, Iseki K, Baba N, Yamaoka G, Arai T, Taminato T Tags: Int J Lab Hematol Source Type: journals

Letter to the Editor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19694837 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - August 18, 2009 Category: Hematology Authors: Gibbs G Tags: Int J Lab Hematol Source Type: journals

Cotransplantation of HLA-identical mesenchymal stem cells and hematopoietic stem cells in Chinese patients with hematologic diseases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mesenchymal stem cells (MSCs) may be employed to support hematopoietic reconstitution and mitigate graft-vs.-host disease (GVHD) in transplantation of hematopoietic stem cells (HSCs). The aim of this study was to explore the feasibility and safety of cotransplantation culture-expanded MSCs and HSCs from the same human leukocyte antigen (HLA)-identical sibling donor in Chinese patients with hematologic diseases. Bone marrow mononuclear cells from healthy donors were cultured and expanded ex vivo. Immunophenotype, adipogenic and osteogenic differentiation potential, and karyotype of the harvested MSCs were detected on th...
Source: International Journal of Laboratory Hematology - July 27, 2009 Category: Hematology Authors: Zhang X, Li JY, Cao K, Lu H, Hong M, Qian S, Wu H, Liu P, Xu W Tags: Int J Lab Hematol Source Type: journals

Telomerase activity and telomere length in acute leukemia: correlations with disease progression, subtypes and overall survival.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The progressive shortening of telomeres and the activation of telomerase are considered to be one of the important mechanisms in cellular immortalization and disease progression. Bone marrow samples were collected from 148 patients with acute leukemia (AL). Based on the stage of the disease, patients were divided into the newly diagnosed group, the relapsed group and the complete remission (CR) group. telomerase activity (TA) was examined by PCR-ELISA, and telomere length (TL) was examined by Southern blot analyses. TA and TL were analyzed in relation to AL stage and subtype. Five-year survival was analyzed using Kapla...
Source: International Journal of Laboratory Hematology - July 14, 2009 Category: Hematology Authors: Wang Y, Fang M, Sun X, Sun J Tags: Int J Lab Hematol Source Type: journals

Establishment of a reference interval for natural killer cell activity through flow cytometry and its clinical application in the diagnosis of hemophagocytic lymphohistiocytosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recently, the Histiocyte Society revised the diagnostic criteria for hemophagocytic lymphohistiocytosis (HLH) to include low or absent natural killer (NK) cell activity, according to local laboratory reference. The aim of this study was to establish reference interval for functional NK-cell activity in 63 healthy Korean individuals using a flow-cytometric assay. We used peripheral blood mononuclear cells (PBMCs) as effector cells and Fluorescein isothiocyanate-labeled K562 cells as target cells. NK-cell activity was calculated using the following equation: NK-cell activity (%) = (test lysis - spontaneous lysis) x 100/(...
Source: International Journal of Laboratory Hematology - July 14, 2009 Category: Hematology Authors: Chung HJ, Park CJ, Lim JH, Jang S, Chi HS, Im HJ, Seo JJ Tags: Int J Lab Hematol Source Type: journals

Anti-polymorphonuclear neutrophil antibodies in patients with leukopenia or neutropenia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Our results suggest that diagnosis of immune Np in the laboratory may be improved by focusing on patient's PMN together with the assessment of cellular markers. PMID: 19594833 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 5, 2009 Category: Hematology Authors: Riera NE, Rosso Saltó M, Galán V, Canalejo K, Khoury M, Aixalá M, Kantor GL, Vermeulen M, Bengió R, DE Bracco MM Tags: Int J Lab Hematol Source Type: journals

Myeloablative hematopoietic stem cell transplantation for myelodysplastic syndrome in patients younger than 55 years: impact of comorbidity and disease burden on the long-term outcome.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We retrospectively investigated 31 myelodysplastic syndrome (MDS) patients receiving myeloablative hematopoietic stem cell transplantation (HCT) and focused on prognostic factors affecting the long-term outcome. Patients were classified according to the French-American-British classification and the HCT-comorbidity index was determined. Cytosine arabinoside or thiotepa combined with cyclophosphamide and total body irradiation was used as myeloablative conditioning in eight and 23 patients respectively. After a follow-up period of 0.8-14.2 years from transplantation (median: 6.4 years), 23 patients were alive in complet...
Source: International Journal of Laboratory Hematology - June 30, 2009 Category: Hematology Authors: Kanamori H, Enaka M, Ito S, Motohashi K, Hagihara M, Oshima R, Sakai R, Fujisawa S, Tanaka M, Fujimaki K, Fujita H, Ishigatsubo Y, Maruta A Tags: Int J Lab Hematol Source Type: journals

Myelodysplastic syndromes: the role of flow cytometry in diagnosis and prognosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Flow cytometric immunophenotypic analysis is a well-recognized and widely accepted adjuvant test to increase the sensitivity and specificity of diagnosis in potential myelodysplastic syndrome (MDS) cases. In addition, flow cytometric analysis provides prognostic information in MDS that is not available from other sources. The flow cytometric findings indicative of MDS and its value is a diagnostic adjunct are discussed. PMID: 19555363 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 24, 2009 Category: Hematology Authors: Stetler-Stevenson M, Yuan CM Tags: Int J Lab Hematol Source Type: journals

Evaluating glutathione S-Transferase (GST) null genotypes (GSTT1 and GSTM1) as a potential biomarker of predisposition for developing leukopenia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Glutathione S-transferase (GST) enzymes protect cells against xenobiotics and oxidative stress products through an electrophilic conjugation process. We investigated the theta (GSTT1) and mu (GSTM1) null genotypes in a group of leukopenic subjects and normal subjects from Northeast Brazil, evaluating their use as biomarkers of susceptibility for developing leukopenia. In a sample-based case-control study, we analysed white blood cell (WBC) counts and GSTT1 and GSTM1 genotypes. A total of 278 subjects were analysed: 91 with leukopenia and 187 controls. GSTT1 null genotype conferred a 5.92-fold risk for occurrence of leu...
Source: International Journal of Laboratory Hematology - June 22, 2009 Category: Hematology Authors: Goncalves MS, Moura Neto JP, Souza CL, Melo P, Reis MG Tags: Int J Lab Hematol Source Type: journals

BAALC and ERG expression in acute myeloid leukemia with normal karyotype: impact on prognosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cytogenetic aberrations are important prognostic factors in acute myeloid leukemia (AML). About 45% of de novo AML lack cytogenetic abnormalities, so identification of predictive molecular markers might improve therapy. We studied the prognostic impact of brain and acute leukemia, cytoplasmic (BAALC) and ETS-related gene (ERG) expression in AML with normal karyotype. Pretreatment bone marrow samples from 30 cytogenetically normal AML patients were analysed for BAALC and ERG expression using real time RT-PCR. The patients were dichotomized at BAALC and ERG mean expression into low and high expression. BAALC showed high ...
Source: International Journal of Laboratory Hematology - June 22, 2009 Category: Hematology Authors: Eid MA, Attia M, Abdou S, El-Shazly SF, Elahwal L, Farrag W, Mahmoud L Tags: Int J Lab Hematol Source Type: journals

Photochemical decomposition of dipyridamole in aqueous solution and the utilization of citrate-theophylline-adenosine-dipyridamole anticoagulant for monitoring of heparin.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19552650 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 21, 2009 Category: Hematology Authors: van den Besselaar AM Tags: Int J Lab Hematol Source Type: journals

Quantitation of whole blood Epstein-Barr virus DNA is useful for assessing treatment response in patients with non-Hodgkin's lymphoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion. These results suggest that whole blood EBV-DNA quantitation might be of value as a convenient biomarker for therapeutic responsiveness of NHL. PMID: 19522857 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 11, 2009 Category: Hematology Authors: Jo SA, Hwang SH, Kim SY, Shin HJ, Chung JS, Sol MY, Chang CL, Lee EY Tags: Int J Lab Hematol Source Type: journals

Abstracts of the XXII International Symposium on Technological Innovations in Laboratory Hematology. May, 11-14, 2009. Las Vegas, Nevada, USA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: PMID: 19413880 [PubMed - indexed for MEDLINE] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 31, 2009 Category: Hematology Tags: Int J Lab Hematol Source Type: journals

Berend Houwen Memorial Lecture: ISLH Las Vegas May 2009: the pathogenesis and management of thrombotic microangiopathies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Thrombotic microangiopathies are a relatively rare group of congenital and inherited disorders caused by defects in processing the ultra large forms of von Willibrand factor which pathologically give rise to platelet rich microthrombi in the micro arterial circulation leading to end organ damage particularly in the brain, heart and kidneys. Identification of the ADAMTS 13 gene has led to the definition of congenital deficiency of its activity or failure of activity due to the development of an inhibitory IgG antibody. The idiopathic autoimmune form of the disease is the most common. There are various subgroups of acqui...
Source: International Journal of Laboratory Hematology - May 31, 2009 Category: Hematology Authors: Scully MA, Machin SJ Tags: Int J Lab Hematol Source Type: journals

Quality counts: new parameters in blood cell counting.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recently several parameters have been introduced to the complete blood count such as nucleated red blood cells, immature granulocytes; immature reticulocyte fraction, immature platelet fraction and red cell fragments as well as new parameters for detection of functional iron deficiency. Leucocyte positional parameters, which may diagnose specific diseases (e.g. differentiate between abnormal lymphocytes in leukaemia and viral conditions and may also detect malarial infection) are now available. At this time they are only used for research; however, generally such parameters later become reportable. One manufacturer's r...
Source: International Journal of Laboratory Hematology - May 31, 2009 Category: Hematology Authors: Briggs C Tags: Int J Lab Hematol Source Type: journals

Immunophenotypic analysis of cerebrospinal fluid cell populations with the Cell-Dyn Sapphire haematology analyser: method feasibility and preliminary observations.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary Cerebrospinal fluid (CSF) samples (n = 50) from patients with neurological disease (bacterial infection, viral infection, neuroborreliosis and multiple sclerosis) were analysed to characterize cell populations by fluorescent immunocytometry with the CD-Sapphire haematology analyser. Reagent combinations applied to all CSF samples comprised CD3/CD19/HLA-DR and CD4/CD8, with some being further analysed using CD3/CD4, CD3/CD16 and CD3/CD25 protocols. Of the 50 samples, 11 were excluded because of high proportions of nonviable cells (n = 2) or insufficient cell numbers (n = 9). Apart from bacterial infection with g...
Source: International Journal of Laboratory Hematology - May 31, 2009 Category: Hematology Authors: Adam P, Sobek O, Scott CS, Dolezil D, Kasik J, Hajdukova L, Adam D Tags: Int J Lab Hematol Source Type: journals

International Normalized Ratio (INR), coagulation factor activities and calibrated automated thrombin generation - influence of 24 h storage at ambient temperature.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary International Normalized Ratio (INR) measurements are used to monitor oral anticoagulation therapy with coumarins. Single coagulation factor activities and calibrated automated thrombin (CAT) generation are considered as more advanced methods for evaluating overall haemostatic capacity. The aims were to assess the variability of INR, coagulation factor activities, and CAT, during 24 h of storage of blood samples at ambient temperature. A total of 24 patients on stable coumarin treatment were followed prospectively for 6 weeks. INR was analyzed at 0, 6 and 24 h after blood sampling and 1-stage clotting activity ...
Source: International Journal of Laboratory Hematology - May 25, 2009 Category: Hematology Authors: Christensen TD, Jensen C, Larsen TB, Maegaard M, Christiansen K, Sørensen B Tags: Int J Lab Hematol Source Type: journals

Genomic analysis of acute leukemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Acute leukemia is the commonest childhood cancer and a major cause of morbidity from hematologic malignancies in adults. Acute lymphoblastic leukemia (ALL) is commonest in children, and acute myeloid leukemia (AML) is more frequent in adults. Apart from childhood ALL, the prognosis of acute leukemia is suboptimal, with many patients experiencing relapse, which carries a poor prognosis, or toxicities from nonspecific therapies. Recent years have witnessed great interest in the application of high-resolution, genome wide approaches to the study of acute leukemia. These studies have identified multiple novel genetic alter...
Source: International Journal of Laboratory Hematology - May 17, 2009 Category: Hematology Authors: Mullighan CG Tags: Int J Lab Hematol Source Type: journals

Real-time PCR quantification of haematopoietic chimerism after transplantation: a comparison between TaqMan and hybridization probes technologies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to compare the sensitivity and accuracy of two methods of quantitative real-time polymerase chain reaction (qrt-PCR), in order to determine haematopoietic chimerism (CH): single nucleotide polymorphisms using TaqMan (TM) probes and insertion/deletion polymorphisms using Hybridization (Hyb) probes. A total of 106 samples from 20 patients who underwent allogenic stem cell transplantation (n = 14) or live-donor liver transplantation (n = 6) were studied. The mean level of chimerism was 8.37% for the TM method and 7.73% in the Hyb method, which was not significantly different (P = 0.69). The Pearson correlatio...
Source: International Journal of Laboratory Hematology - May 11, 2009 Category: Hematology Authors: Martinez-Lopez J, Crooke A, Grande S, Ayala R, Jiménez-Velasco A, Gamarra S, Meneu JC, Gilsanz F Tags: Int J Lab Hematol Source Type: journals

Investigation of the distribution of lymphocyte subsets and zinc levels in multitransfused beta-thalassemia major patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to utilize flow cytometric immunophenotyping to characterize effects of regular blood transfusion, and high serum ferritin levels because of irregular use of iron chelation therapy on T lymphocytes (CD2, CD3, CD4 and CD8), B lymphocytes (CD19) and natural killer cells (CD56) and zinc levels in the blood of patients with thalassemia major (n = 49) and healthy normal controls (n = 60) in Kuwait. None of the patients had active infections. T-cell markers' percentage levels were comparable between patients and controls (P > 0.05), while B cell marker (CD19) was significantly higher in patients (P = 0...
Source: International Journal of Laboratory Hematology - April 21, 2009 Category: Hematology Authors: Al-Awadhi AM, Alfadhli SM, Al-Khaldi D, Borhama M, Borusly M Tags: Int J Lab Hematol Source Type: journals

A novel hemoglobin variant beta135(H13) Ala > Asp identified in an asymptomatic Korean family by direct sequencing: suggesting a new insight into Hb Beckman mutation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This article describes the clinical observation of a novel hemoglobin (Hb) variant found during the course of routine blood testing on a 61-year-old subject. The Hb variant was observed during HbA1c testing by ion-exchange high-performance liquid chromatography. Alkaline electrophoresis and DNA sequencing confirmed the presence of a new Hb variant, HBB:c.407C > A (p.Ala136Asp). This mutation has been reported to induce Hb Beckman variant in the Globin Gene Server. However, it was different from the only experimental report for Hb Beckman by Rahbar, Lee & Asmeron (p.Ala136Glu; Hb Beckman alpha2 beta2 135(H13) ala-to-...
Source: International Journal of Laboratory Hematology - April 21, 2009 Category: Hematology Authors: Kim SY, Kim GY, Jo SA, Lee EH, Cho EH, Hwang SH, Lee EY Tags: Int J Lab Hematol Source Type: journals

Evaluating five dedicated automatic devices for haemoglobinopathy diagnostics in multi-ethnic populations.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary We have tested five haemoglobin (Hb) separation apparatuses, dedicated to haemoglobinopathy diagnostics. These are the four high performance liquid chromatography devices: VARIANT II(), HA 8160, G7, Ultra(2) and the Capillary Electrophoresis apparatus from Sebia. In the first place, we focussed on the capacity of all apparatuses to detect the most common structural variants relevant for public health, these being HbS, HbC, HbE, HbD-Punjab and HbO-Arab. We then compared how the high HbA(2)beta-thalassaemia carriers were identified. All apparatuses were able to identify carriers of these traits with the expected ...
Source: International Journal of Laboratory Hematology - April 16, 2009 Category: Hematology Authors: VAN Delft P, Lenters E, Bakker-Verweij M, de Korte M, Baylan U, Harteveld CL, Giordano PC Tags: Int J Lab Hematol Source Type: journals

Multiple myeloma presenting with an acute bacterial infection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary Increased susceptibility to bacterial infections is a common manifestation of multiple myeloma (MM), arising mainly from a defect in humoral immunity and is associated with major morbidity and mortality. The propensity to infection is increased in the first months after the initial diagnosis and in patients with renal dysfunction. Gram-positive infections, mainly pneumonia from Streptococcus pneumoniae, occur more frequently in patients with untreated disease while Gram-negative infections, mainly of the urinary tract, are more common after chemotherapy. However, an acute bacterial infection is rarely reported ...
Source: International Journal of Laboratory Hematology - April 16, 2009 Category: Hematology Authors: Kalambokis GN, Christou L, Tsianos EV Tags: Int J Lab Hematol Source Type: journals

Clinical and haematological features in a compound heterozygote (HBB:c.92 + 5G > C/HBB:c.93-2A > C) case of thalassaemia major.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This report confirms the presence of HBB:c.93-2A > C in the Indian subcontinent and has important implications for screening and prenatal diagnosis of beta thalassaemia. This report also supports inclusion of this mutation in the beta globin gene mutation database. PMID: 19486366 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - April 12, 2009 Category: Hematology Authors: Agarwal S, Tamhankar PM, Kumar R, Dalal A Tags: Int J Lab Hematol Source Type: journals

Clinicovirologic analysis of hepatitis C infection in transfusion-dependent beta-thalassemia major children.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Regular blood transfusion puts beta-thalassemia major patients at a higher risk of developing hepatic iron overload and hepatitis C virus (HCV) infection. The association between several transfusion-related factors and an increased risk of developing HCV viremia has been reported. The effect of HCV infection on liver damage in transfusion-dependent thalassemia patients has been poorly described. A sample of 100 Egyptian transfusion-dependent beta-thalassemia major children were studied. Individual patients underwent full history taking, clinical examination and a panel of laboratory tests including HCV ribonucleic acid...
Source: International Journal of Laboratory Hematology - April 5, 2009 Category: Hematology Authors: Ragab L, Helal S, Zaghloul N, El-Raziky M, Afifi R, Musallam KM, Taher AT Tags: Int J Lab Hematol Source Type: journals

British Society for Haematology, slide session, annual scientific meeting, Glasgow, 2008.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A morphology session is held each year at the Annual Scientific Meeting of the British Society of Haematology. Prior to the meeting this year, eight morphology cases were made available to BSH members as glass slides and also digitally as 'virtual slides'. A panel of invited commentators who had no prior knowledge of the diagnosis discussed the eight cases. An initial limited history and blood count are given with representative images from the case material; this is followed by the discussants' comments and suggested diagnosis. The actual clinical diagnosis is then given with other relevant information. PMID: 1936...
Source: International Journal of Laboratory Hematology - April 1, 2009 Category: Hematology Authors: Hutchinson CV, Burthem J, Bisland M, Carey P, Crotty G, Devalia V, Janda B, Gordon W, Harrison CN, Murray J, Shlebak A, Thomas A, Wilkins B, McMullin MF Tags: Int J Lab Hematol Source Type: journals

Performance evaluation of the PENTRA 60C+ automated hematology analyzer and comparison with the ADVIA 2120.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The PENTRA 60C(+) hematology analyzer provides a complete blood cell (CBC) count, including a five-part differential (5-DIFF) count and two leukocyte subpopulations, i.e. large immature cells (LIC's) and atypical lymphocytes (ALY's). We evaluated its analytical performance and assessed agreement with the ADVIA 2120, in order to install the analyzer in a small satellite hematology laboratory. First we assessed repeatability, reproducibility and carry-over to evaluate the analytical performance. Then we used Pearson correlation coefficients, Passing and Bablok regression analysis and a graphical approach (n = 209) to eva...
Source: International Journal of Laboratory Hematology - March 11, 2009 Category: Hematology Authors: Guerti K, Vertessen F, Daniëls L, Van Der Planken M Tags: Int J Lab Hematol Source Type: journals

False positive Kleihauer results: an unusual cause in a postnatal patient in remission from acute myeloid leukaemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A 34-year-old woman, in remission from acute myeloid leukaemia, had a positive postnatal Kleihauer result. Following our standard local protocol for positive Kleihauer tests in Rh-D negative patients, the specimen was referred for confirmatory testing, which proved the result to be false positive. Hereditary persistence of foetal haemoglobin was excluded as a Kleihauer test performed in a pregnancy prior to the development of leukaemia was negative. Further testing confirmed mild elevation of foetal haemoglobin (HbF) and increased F-cells. Elevated-HbF levels have been widely reported in patients with haematological ma...
Source: International Journal of Laboratory Hematology - March 11, 2009 Category: Hematology Authors: Grant R, Keidan J Tags: Int J Lab Hematol Source Type: journals

Preservation of basophils in dapsone-induced agranulocytosis suggests a possible pathogenetic role for leucocyte peroxidases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a hitherto unreported laboratory observation, namely the selective preservation of basophils, in a case of severe dapsone-induced agranulocytosis. Dapsone is known to be metabolised by peroxidases to nitroso derivatives in non-hemopoietic cells where these can in turn act as haptens. Our observation that basophils are selectively spared in this syndrome supports the hypothesis that leucocyte peroxidases function in a similar way to facilitate the pathogenesis of this form of agranulocytosis in susceptible individuals. PMID: 19267812 [PubMed - in process] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - March 11, 2009 Category: Hematology Authors: Besser M, Vera J, Clark J, Chitnavis D, Beatty C, Vassiliou G Tags: Int J Lab Hematol Source Type: journals

M/H ratio for the differential diagnosis of microcytic anemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19267813 [PubMed - in process] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - March 11, 2009 Category: Hematology Authors: Ntaios G, Chatzinikolaou A Tags: Int J Lab Hematol Source Type: journals

Clinical and hematological study for Parvovirus b19 infection in children with acute leukemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to detect parvovirus B19 DNA together with its antibodies in the sera of children with recent acute leukemia and those with acute leukemia receiving chemotherapy to clarify the contribution of this infection to changes observed in hematological and clinical presentations in these populations. Two groups were included: Group I comprised 45 children with acute leukemia receiving chemotherapy and Group II comprised 40 children with recently diagnosed acute leukemia. Serum parvovirus B19 IgG and IgM were investigated by enzyme-linked immunosorbant assay and the virus DNA was sought by polymerase chain reaction...
Source: International Journal of Laboratory Hematology - February 26, 2009 Category: Hematology Authors: Zaki ME, Ashray RE Tags: Int J Lab Hematol Source Type: journals

International Council for Standardization in Haematology - the first 40 years.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Summary This brief history of the origin and development of ICSH must, inevitably, be selective as it has been extracted from the many pages of the records of the meetings of the ICSH board and its secretariat, the annual assembly and the reports of the various expert panels and working groups. It is hoped that it will give a picture of the way in which ICSH functioned and the many experts around the world who have contributed to its activities - but with an apology and appreciation, to other colleagues who have not been named in this annotation, but who made significant contributions to the activities that are describ...
Source: International Journal of Laboratory Hematology - February 26, 2009 Category: Hematology Authors: Lewis SM Tags: Int J Lab Hematol Source Type: journals

Letter to the Editor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19254347 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - February 25, 2009 Category: Hematology Authors: O'Broin S, McCarthy N Tags: Int J Lab Hematol Source Type: journals

The effects of proteasome inhibitor bortezomib on a P-gp positive leukemia cell line K562/A02.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study is to clarify the efficacy of proteasome inhibitor bortezomib to multidrug resistant (MDR) acute leukemia cells. We observed the effects of bortezomib on a P-glycoprotein (P-gp) positive leukemia line K562/A02. The results showed that bortezomib has significant effects on P-gp positive K562/A02 cells including cytotoxicity (48 h IC(50): 171.36 nm), induction of apoptosis (31.71 +/- 1.07% apoptotic cells after 24 h treatment at 100 nm), and inhibition of proteasome chymotrypsin-like activity (relative activity to untreated controls: 20.07 +/- 0.66% at 24 h with 10 nm bortezomib). These effects were...
Source: International Journal of Laboratory Hematology - February 25, 2009 Category: Hematology Authors: Lü S, Chen Z, Yang J, Chen L, Zhou H, Xu X, Li J, Han F, Wang J Tags: Int J Lab Hematol Source Type: journals