International Journal of Molecular MedicineThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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508 records returned

Ghrelin in gastrointestinal diseases and disorders: a possible role in the pathophysiology and clinical implications (review).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ghrelin is a peptide hormone, which has been isolated from the stomach. It is localized mostly in endocrine cells in the oxyntic mucosa of the stomach. Ghrelin receptors are expressed equally in all parts of the gastrointestinal tract, with a similar level of expression in the mucosal and muscle layers. This peptide hormone has several functions, the most widely known is its growth hormone (GH)-releasing effect. Ghrelin plays an important role in regulating appetite, feeding and energy metabolism. It also plays a role in mediating immune response and inflammatory processes. Ghrelin stimulates gastric motility and empty...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: El-Salhy M Tags: Int J Mol Med Source Type: journals

Diagnosis of parathyroid carcinoma using immunohistochemical staining against hTERT.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The differential diagnosis of parathyroid carcinoma from benign adenoma is often difficult when its typical clinicopathological features are absent, even with the aid of various molecular markers. We recently demonstrated that telomerase activation through hTERT expression is a unique characteristic that is limited to parathyroid carcinoma and not seen in benign tumors. In the present study, we investigated hTERT expression in parathyroid tumors using immunohistochemistry in an attempt to determine its clinical utility. There was no evidence of immunoreactivity in the 4 normal parathyroid glands and the 18 typical aden...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Osawa N, Onoda N, Kawajiri H, Tezuka K, Takashima T, Ishikawa T, Miyauchi A, Hirokawa M, Wakasa K, Hirakawa K Tags: Int J Mol Med Source Type: journals

Neuronal and BBB damage induced by sera from patients with secondary progressive multiple sclerosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An important component of the pathogenic process of multiple sclerosis (MS) is the blood-brain barrier (BBB) damage. We recently set an in vitro model of BBB, based on a three-cell-type co-culture system, in which rat neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological BBB. Herein we report that the serum from patients with secondary progressive multiple sclerosis (SPMS) has a damaging effect on isolated neurons. This finding suggests that neuronal damaging in MS could be a primary event and not only secon...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Proia P, Schiera G, Salemi G, Ragonese P, Savettieri G, Di Liegro I Tags: Int J Mol Med Source Type: journals

Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a novel solid-state nuclear magnetic resonance (NMR) method that maximizes the advantages of high-resolution magic-angle-spinning (HRMAS), relative conventional liquid-state NMR approaches, when applied to intact biopsies of skeletal muscle specimens collected from burn trauma patients. This novel method, termed optimized adiabatic TOtal through Bond correlation SpectroscopY (TOBSY) solid-state NMR pulse sequence for two-dimensional (2D) 1H-1H homonuclear scalar-coupling longitudinal isotropic mixing, was demonstrated to provide a 40-60% improvement in signal-to-noise ratio (SNR) relative to its liquid-state an...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Righi V, Andronesi O, Mintzopoulos D, Tzika AA Tags: Int J Mol Med Source Type: journals

Human platelet 12-lipoxygenase: naturally occurring Q261/R261 variants and N544L mutant show altered activity but unaffected substrate binding and membrane association behavior.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The single nucleotide polymorphism (SNP) R261Q in the human platelet 12-lipoxygenase has been correlated with several human diseases. To understand better the biological performance we have compared enzymatic properties of the recombinant enzymes: 'wild-type' as Q261 and R261 variants with a single Q261R mutation at the enzyme periphery and N544L mutant with an altered active site. The R261 variant does not follow the same kinetics such as WT-Q261 showing a lag phase, a slower accumulation of product, following a different time-course without reaching plateau characteristic for the Q261 variant. The N544L substitution ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Aleem AM, Wells L, Jankun J, Walther M, Kühn H, Reinartz J, Skrzypczak-Jankun E Tags: Int J Mol Med Source Type: journals

Evaluation of annexin II as a potential serum marker for hepatocellular carcinoma using a developed sandwich ELISA method.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Annexin II (Annexin A2, ANXA2) is a 36 kDa calcium-dependent phospholipid-binding protein that is located on the surface of most eukaryotic cells. ANXA2 is involved in several biological processes, including anti-inflammatory effects, Ca27+-dependent exocytosis, immune responses, Ca2+ transport and phospholipase A2 regulation. In our previous study, ANXA2 was identified as an up-regulated gene in hepatocellular carcinoma (HCC) tissue by cDNA microarray. In the present study, we have evaluated ANXA2 as a tumor-associated marker of HCC. We determined the ANXA2 levels in human liver tissues with HCC using real-time RT-PCR...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Ji NY, Park MY, Kang YH, Lee CI, Kim DG, Yeom YI, Jang YJ, Myung PK, Kim JW, Lee HG, Kim JW, Lee K, Song EY Tags: Int J Mol Med Source Type: journals

Experimental study of the anti-cancer mechanism of tanshinone IIA against human breast cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was performed to determine the anti-cancer activity of tanshinone IIA on human breast cancer cells in vitro and in vivo and to elucidate the underlying mechanism of this activity. Human breast cancer cell lines (estrogen receptor-positive and -negative) were treated with tanshinone IIA and tamoxifen. The inhibitory effects of tanshinone IIA and tamoxifen on breast cancer cell proliferation were examined using MTT assays, BrdU incorporation, immunohistochemistry and flow cytometry. Upon treatment with tanshinone IIA, breast cancer cell proliferation was significantly inhibited in a dose- and time-dependent manner...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Lu Q, Zhang P, Zhang X, Chen J Tags: Int J Mol Med Source Type: journals

Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Neuropeptides B (NPB) and W (NPW) have been identified as endogenous ligands of two G-protein-coupled receptors, neuropeptides B/W receptor 1 (NPBWR1, formerly known as GPR7) and neuropeptides B/W receptor 2 (NPBWR2, formerly known as GPR8). In rodents where NPBWR2 is absent, its counterpart is named the similar to neuropeptides B/W receptor 2 (similar to NPBWR2, formerly GPR8-like). Both NPB and NPW play a role in the control of feeding, neuroendocrine axis functions, memory and learning processes as well as in pain regulation. The present study aimed to investigate the expression of NPB, NPW, NPBWR1 and the similar t...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Ziolkowska A, Rucinski M, Tyczewska M, Malendowicz LK Tags: Int J Mol Med Source Type: journals

REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The reduced expression in immortalized cells (REIC)/Dickkopf (Dkk)-3, a member of the Dkk gene family, is a tumor suppressor in a broad range of cancers. REIC/Dkk-3 transfected stable clones of mouse prostate cancer RM9 cells (RM9-REIC) and the empty vector-transfected control clone cells (RM9-EV) were established. Clones were used to evaluate the anti-cancer effects and a proteomics analysis of REIC/Dkk-3 continuous expression was performed. The RM9-REIC cells show a feeble appearance and the cell membrane shows irregular buds known as blebs. In vitro cell proliferation was significantly suppressed in RM9-REIC clones ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Chen J, Watanabe M, Huang P, Sakaguchi M, Ochiai K, Nasu Y, Ouchida M, Huh NH, Shimizu K, Kashiwakura Y, Kaku H, Kumon H Tags: Int J Mol Med Source Type: journals

Dysfunction of Ca2+/CaM kinase IIalpha cascades in the amygdala in post-traumatic stress disorder.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated changes in Ca2+-CaM-CaMKIIalpha in the basolateral amygdala of rats after SPS which may reveal part of the pathogenesis of PTSD. The intracellular free calcium level in the basolateral amygdala was examined by fluorescence spectrophotometry. CaM and CaMKIIalpha expression in basolateral amygdala was examined using immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction. The intracellular free calcium level in the basolateral amygdala was increased when compared to that in the control group 1 day after SPS exposure (P<0.05). CaM expression significantly in...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Xiao B, Han F, Shi YX Tags: Int J Mol Med Source Type: journals

Lambda-carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Previous studies have shown that highly sulfated lambda-carrageenan oligosaccharides (lambda-CO) possess an anti-angiogenetic effect, while high concentrations of lambda-CO present a cytotoxic effect towards human umbilical vein endothelial cells (HUVECs). The aim of this study was to explore the underlying mechanism of lambda-CO on inhibiting cell proliferation. lambda-CO elicited reactive oxygen species (ROS) production with concentrations at 0.8 and 1 mg/ml, and this event was accompanied by the increase of early apoptotic cells, nuclear morphology changes and cell cycle arrest at the S and G2/M phases. However, pre...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Chen HM, Yan XJ, Mai TY, Wang F, Xu WF Tags: Int J Mol Med Source Type: journals

Neurotrophic receptor tyrosine kinase B induces c-fos-associated cell survival.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we established 293T cells stably expressing human TrkB to elucidate its intracellular functions. Using this cell system, we examined the biological roles of TrkB and its downstream target molecules. The TrkB expressing cells showed an increased survival rate through increased c-fos mRNA expression by BDNF, which were completely suppressed by TrkB inhibitor. Moreover, the combination of inhibitors of mitogen-activated protein kinase (MEK) and phosphatidylinositol 3-kinase (PI3K) partially reduced both the cell survival rate and c-fos mRNA expression, whereas monotreatment of these reagents could not affect ce...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Hayashi A, Nakatani K, Nishioka J, Sakamoto Y, Jinda S, Wada H, Nobori T Tags: Int J Mol Med Source Type: journals

Coronary artery disease and depression: possible role of brain-derived neurotrophic factor and serotonin transporter gene polymorphisms.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cardiovascular disease (CVD) and depression are two of the most common human health problems. Patients with depression have an increased risk of developing cardiovascular disease and mortality after experiencing a cardiac event. Both diseases are complex disorders that are influenced by genetic and environmental factors. Brain-derived neuro-trophic factor (BDNF) plays a critical role in regulating both vascular development and response to injury, and promotes survival, differentiation, and maintenance of neurons in the peripheral and nervous system. Evidence suggests that BDNF can enhance serotoninergic transmission. S...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Bozzini S, Gambelli P, Boiocchi C, Schirinzi S, Falcone R, Buzzi P, Storti C, Falcone C Tags: Int J Mol Med Source Type: journals

Glycoprotein extraction from Laminaria japonica promotes IEC-6 cell proliferation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we isolated a novel glycoprotein from L. japonica that stimulates the growth of the IEC-6 normal murine intestinal epithelial cells. We also identified the mechanism by which this glycoprotein, referred to as LJGP, stimulates cell growth. After 24 h of exposure to LJGP, cell proliferation increased in a dose-dependent manner. To further explore the mechanism associated with LJGP-induced cell proliferation, we treated cells for various times with LJGP. We focused on the epidermal growth factor receptor (EGFR) signaling pathway, which is involved in the regulation of cellular proliferation and differentiation,...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Go H, Hwang HJ, Nam TJ Tags: Int J Mol Med Source Type: journals

Changes in the expression of cholesterol metabolism-associated genes in HCV-infected liver: a novel target for therapy?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we evaluated the expression of lipid metabolism-associated genes in patients with HCV infection by real-time PCR. Sterol regulatory element-binding protein (SREBP)-2 expression was unchanged and low density lipoprotein receptor expression was markedly reduced by 90% in HCV-infected liver. The expression of apolipoprotein B100, microsomal triglyceride transfer protein and ATP-binding cassette G5 was significantly increased. Up-regulation of cholesterol synthesis-associated genes, including HMG-CoA reductase, HMG-CoA synthase, farnesyl-diphosphate synthase and squalene synthase, confirmed enhanced de novo chol...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Nakamuta M, Yada R, Fujino T, Yada M, Higuchi N, Tanaka M, Miyazaki M, Kohjima M, Kato M, Yoshimoto T, Harada N, Taketomi A, Maehara Y, Koga M, Nishinakagawa T, Nakashima M, Kotoh K, Enjoji M Tags: Int J Mol Med Source Type: journals

Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Crohn's disease is associated with increased permeability of the intestine even in quiescent patients. Increased intestinal permeability may cause dysregulated immunological responses in the intestinal mucosa that leads to chronic intestinal inflammation. Tight junction proteins contribute to intestinal permeability, and functional abnormality and dislocation of such proteins may cause increased intestinal permeability. We studied the expression of tight junction proteins Rab13, vasodilator-stimulated phosphoprotein (VASP), zonula occludin-1 (ZO-1), and F-actin in the intestinal epithelium of patients with inactive inf...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Ohira M, Oshitani N, Hosomi S, Watanabe K, Yamagami H, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T Tags: Int J Mol Med Source Type: journals

Middle-term expansion of hematopoietic cord blood cells with new human stromal cell line feeder-layers and different cytokine cocktails.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cord blood (CB) is a source of hematopoietic stem cells (HSCs) and is an alternative to bone marrow for allogenic transplantation in patients with hematological disorders. The improvement of HSC in vitro expansion is one of the main challenges in cell therapy. Stromal components and soluble factors, such as cytokines, can be useful to induce in vitro cell expansion. Hence, we investigated whether feeder-layers from new stromal cell lines and different exogenous cytokine cocktails induce HSC expansion in middle-term cultures. CB HSC middle-term expansion was carried out in co-cultures on different feeder-layers exposed ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: De Angeli S, Baiguera S, Del Pup L, Pavan E, Gajo GB, Di Liddo R, Conconi MT, Grandi C, Schiavon O, Parnigotto PP Tags: Int J Mol Med Source Type: journals

The effects of adiponectin on interleukin-6 and MCP-1 secretion in lipopolysaccharide-treated 3T3-L1 adipocytes: role of the NF-kappaB pathway.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It was recently suggested that the transcription nuclear factor-kappaB (NF-kappaB) plays an important role in controlling the inflammation and metabolic alterations associated with obesity. In endothelial and monocytic cells, adiponectin acts as a modulator of the inflammatory response, suppressing NF-kappaB activation. The aim of this study was to assess the ability of different forms of adiponectin to modulate the inflammatory response in adipocytes. 3T3-L1 preadipocytes were cultured according to standard conditions. Fully differentiated adipocytes were stimulated with 1 microg/ml lipopolysaccharides (LPS) for 16 h,...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Zoico E, Garbin U, Olioso D, Mazzali G, Fratta Pasini AM, Di Francesco V, Sepe A, Cominacini L, Zamboni M Tags: Int J Mol Med Source Type: journals

Effects of protein kinase Cdelta and phospholipase C-gamma1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that plays an important role in immune cell migration. It has been reported that chemokines, including MCP-1, are involved in angiogenesis and metastasis. However, the exact role of chemokines in cancer development is still obscure. We investigated the involvement of MCP-1 in taxol-induced breast cancer cell death. The anti-cancer drug taxol induced MCF-7 breast cancer cell death. Treatment with taxol increased the mRNA expression level of MCP-1 in a dose- and time-dependent manner. Up-regulation of MCP-1 by taxol was augmented in cells treated with rottlerin...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Kim YS, An HT, Kim J, Ko J Tags: Int J Mol Med Source Type: journals

The metabolic syndrome of omega3-depleted rats. IV. Intestinal phospholipid omega3 fatty acids.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A dietary deprivation in long-chain polyunsaturated omega3 fatty acids initiated in 7-week old normal rats provokes within 3 to 7 months the appearance of several features of the metabolic syndrome. Likewise, within 2 to 4-5 weeks exposure to a flaxseed oil-enriched diet, these anomalies are rapidly corrected. The present study deals with the omega3 fatty acid content of intestinal phospholipids under the same experimental conditions. For the sake of comparison, the control rats were given access during the last 4-5 weeks to either a soybean or flaxseed oil-enriched diet. In control rats, the relative weight content of...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Hacquebard M, Portois L, Malaisse WJ, Carpentier YA Tags: Int J Mol Med Source Type: journals

The metabolic syndrome of omega3-depleted rats. V. Intestinal phospholipid omega6 fatty acids.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aims mainly at investigating the effects of a dietary deprivation and replenishment of omega3 PUFA upon the phospholipid pattern of omega6 PUFA in the duodenum, jejunum, caecum and colon of rats exposed for 3-7 months to an omega3-depleted diet and then eventually exposed for 2-4 weeks to an omega3-rich diet. In control rats, the relative weight content of all omega6 fatty acids differed in the proximal and distal intestinal segments. In the omega3-depleted rats the C18:2omega6, C20:2omega6 and C20:3omega6 content was decreased whilst that of C20:4omega6 and C22:4omega6 was increased. Significant correlations we...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Hacquebard M, Portois L, Malaisse WJ, Carpentier YA Tags: Int J Mol Med Source Type: journals

Bone morphogenetic proteins in development and progression of breast cancer and therapeutic potential (review).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bone morphogenetic proteins (BMPs) belong to the TGF-beta superfamily, which plays important roles in foetal and postnatal development and also maintains the homeostasis of various tissues and organs. Due to the critical role played by BMPs in bone formation and bone turnover, the implication of these molecules in bone metastasis has been intensively studied over the past decade. BMPs have been implicated in the development and progression of solid tumours, particularly the disease-specific bone metastasis. In breast cancer, a tumour type which most commonly metastasizes to bones, aberrations of both BMP expression and...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Ye L, Bokobza SM, Jiang WG Tags: Int J Mol Med Source Type: journals

STEAP4 regulates focal adhesion kinase activation and CpG motifs within STEAP4 promoter region are frequently methylated in DU145, human androgen-independent prostate cancer cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we report that STEAP4 expression is able to inhibit anchorage-independent cell growth. We also demonstrate that STEAP4 associates with focal adhesion kinase (FAK) and regulate the activity of FAK through Y397 phosphorylation. Furthermore, we show that CpG sequences in STEAP4 promoter region were frequently methylated in DU145, androgen-independent prostate cancer cells. Demethylation treatment induced STEAP4 expression in DU145, suggesting the possibility that STEAP4 expression in cancer cells is in part epigenetically regulated. Collectively, these data demonstrate a novel function of STEAP4 and that STEAP4...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Tamura T, Chiba J Tags: Int J Mol Med Source Type: journals

Role of Kenae/CCDC125 in cell motility through the deregulation of RhoGTPase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we performed immunoscreening using the serum from a patient with Isaac's syndrome and identified the novel gene named Kenae/CCDC125. Expression analysis of Kenae/CCDC125 revealed that its transcript was highly expressed in tissues associated with the immune system, such as the thymus, spleen and bone marrow. In cells stably expressing Kenae/CCDC125, delay in cell motility and deregulation of RhoGTPase (RhoA, Rac1 and cdc42) activity to extracellular stimuli were demonstrated. These results suggest that the novel gene, Kenae/CCDC125, acts as a regulator of cell motility through RhoA, Rac1 and cdc42. PMID:...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Araya N, Arimura H, Kawahara K, Yagishita N, Ishida J, Fujii R, Aratani S, Fujita H, Sato T, Yamano Y, Higuchi I, Osame M, Nishioka K, Fukamizu A, Arimura K, Maruyama I, Nakajima T Tags: Int J Mol Med Source Type: journals

Differences in gene expression between individuals with multiple primary and single primary malignancies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cytogenetic and molecular studies have identified imbalanced chromosomal regions leading to the characterization of several candidate genes. Differences in gene expression were examined in the blood by whole genome microarray analysis among individuals with double or single primary malignancies and healthy individuals. Twenty-four individuals with at least two primary malignancies of the breast and/or colon and/or ovary were compared with 32 individuals with single breast, colon or ovarian cancer. The single malignancy group had a median duration of disease of 9 years (range 5-23 years). Validation was obtained by exam...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Stathopoulos GP, Armakolas A Tags: Int J Mol Med Source Type: journals

Activation of peroxisome proliferator-activated receptor-gamma induces apoptosis on acute promyelocytic leukemia cells via downregulation of XIAP.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study we investigated the in vitro apoptosis inducing effects of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) ligand ciglitazone (CGZ) on acute promyelocytic leukemia (APL) NB4 cells and its mechanisms of action. The results revealed that CGZ (10-50 micromol/l) inhibited the growth of leukemia NB4 cells and caused apoptosis in a time- and dose-dependent manner. Apoptosis was observed clearly by flow cytometry (FCM) and DNA fragmentation analysis. After treatment by CGZ for 48 h, the percentage of disruption of mitochondrial membrane potential (Deltapsim) was increased in a dose-dependent...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Liu JJ, Guo YW, Fang ZG, Si XN, Wu XY, Liu PQ, Lin DJ, Xiao RZ, Xu Y, Wang CZ, Li XD, He Y, Huang RW Tags: Int J Mol Med Source Type: journals

Novel synthetic inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity that inhibit tumor cell proliferation and are structurally unrelated to existing statins.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pilot-scale libraries of eight-membered medium ring lactams (MRLs) and related tricyclic compounds (either seven-membered lactams, thiolactams or amines) were screened for their ability to inhibit the catalytic activity of human recombinant 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase in vitro. A dozen of the synthetic compounds mimic the inhibition of purified HMG-CoA reductase activity caused by pravastatin, fluvastatin and sodium salts of lovastatin, mevastatin and simvastatin in this cell-free assay, suggesting direct interaction with the rate-limiting enzyme of cholesterol biosynthesis. Moreover, seve...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Perchellet JP, Perchellet EM, Crow KR, Buszek KR, Brown N, Ellappan S, Gao G, Luo D, Minatoya M, Lushington GH Tags: Int J Mol Med Source Type: journals

Inhibition of angiogenesis in lipodermatosclerosis: implication for venous ulcer formation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lipodermatosclerosis refers to skin induration of the lower extremities characterized by tortuous, hyperpermeable vessels preceding venous leg ulcerations. Protein ligands and receptor tyrosine kinases that specifically regulate endothelial cell function are mainly involved in physiological as well as in disease-related angiogenesis. These ligand/receptor systems include the vascular endothelial growth factor (VEGF) and the angiopoietin (Ang) families and their receptor the tyrosine kinase with immunoglobulin-like domains (Tie-2) as well as the VEGF receptor family (VEGF-R1 and VEGF-R2). In the present study, the contr...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Herouy Y, Kreis S, Mueller T, Duerk T, Martiny-Baron G, Reusch P, May F, Idzko M, Norgauer Y Tags: Int J Mol Med Source Type: journals

Mitochondria-mediated tumstatin peptide-induced HepG2 cell apoptosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, peptide 19 induced HepG2 cell apoptosis through the mitochondrial apoptosis pathway. PMID: 19787199 [PubMed - in process] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Liu Y, Li J, Xu H, Zhang Y, Liu Y, Liu X Tags: Int J Mol Med Source Type: journals

Copper ions regulate cytotoxicity of disulfiram to myeloid leukemia cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated the cell density-dependent induction of apoptosis of human acute myeloid leukemia U937 and ML-1 cells by disulfiram (DSF), the dithiocarbamate drug recently proposed for treatment of human cancers. This effect is dependent on uptake of extracellular copper and its intracellular accumulation. High-density cells cannot uptake and accumulate this metal to a sufficient level that would allow induction of apoptosis due to progressive decrease of its extracellular concentration. Simple addition of copper can resume sensitivity of high-density leukemic cells to DSF and improve efficiency of anti-leu...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Navrátilová J, Jungová P, Vanhara P, Preisler J, Kanicky V, Smarda J Tags: Int J Mol Med Source Type: journals

The bone anabolic carotenoid beta-cryptoxanthin enhances transforming growth factor-beta1-induced SMAD activation in MC3T3 preosteoblasts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study suggests that the carotenoid beta-cryptoxanthin may promote osteoblast differentiation and activity by amplifying TGF-beta1-induced lineage commitment of osteoblast precursors. PMID: 19787201 [PubMed - in process] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Yamaguchi M, Weitzmann MN Tags: Int J Mol Med Source Type: journals

Frequent expression of the high molecular, 673-bp CD44v3,v8-10 variant in colorectal adenomas and carcinomas.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CD44 is a transmembrane glycoprotein involved in the interaction between cells and the extracellular matrix. A large variety of alternatively spliced CD44 variants are expressed by different tumors with possible implication for tumor progression, formation of metastasis and survival. In colon carcinomas, previous reports described higher molecular bands of CD44 transcripts in neoplastic colonic tissue, although a complete analysis of multiple combinations of CD44v transcripts were not performed. We therefore analyzed the pattern of CD44 standard and variant (v2-v10) transcripts in colorectal adenomas and carcinomas by ...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Kopp R, Fichter M, Schalhorn G, Danescu J, Classen S Tags: Int J Mol Med Source Type: journals

Suppression of tissue inhibitor of metalloproteinase-1 by recombinant adeno-associated viruses carrying siRNAs in hepatic stellate cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Elevated tissue inhibitor of metalloproteinase (TIMP)-1 expression contributes to excess production of extracellular matrix in liver fibrosis. However, there are few studies on sustained suppression of TIMP-1. We aimed to construct a recombinant adeno-associated virus (AAV) carrying small interfering RNAs (siRNAs) of TIMP-1 and investigate the long-term effects of RNA interference upon the TIMP-1 gene in rat hepatic stellate cells (HSCs). Five siRNA oligomers targeting rat TIMP-1 were designed and transfected into HSCs. A U6 promoter followed by the siRNA which had the strongest suppression effect was cloned into the A...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Cong M, Liu T, Wang P, Xu Y, Tang S, Wang B, Jia J, Liu Y, Hermonat PL, You H Tags: Int J Mol Med Source Type: journals

Implications of antioxidant enzymes in human gastric neoplasms.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study is the first to evaluate the expression and activity of MnSOD, Cu/ZnSOD and catalase in human gastric samples, since ROS play a significant role in the pathogenesis of different forms of malignancy inducing mutations and various diseases such as gastric cancer. Biopsies and surgical samples from 53 patients (male/female 22/31, mean age 56.5+/-15.8 years) consisted of 15 healthy, 12 autoimmune atrophic gastritis, 10 Helicobacter pylori (HP) infection, 8 HP-negative chronic gastritis (CG) and 8 adenocarcinoma cases. Enzyme activity and expression were evaluated by spectrophotometry and immunoblotting af...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Monari M, Foschi J, Calabrese C, Liguori G, Di Febo G, Rizzello F, Gionchetti P, Trinchero A, Serrazanetti GP Tags: Int J Mol Med Source Type: journals

Association of genetic variants with myocardial infarction in individuals with or without hypertension or diabetes mellitus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hypertension and diabetes mellitus are important risk factors for myocardial infarction (MI). The purpose of the present study was to identify genetic variants that confer susceptibility to MI in individuals with or without hypertension or diabetes mellitus, thereby contributing to the personalized prevention of MI in such individuals. The study population comprised 5,835 unrelated Japanese individuals, including 1,339 subjects with MI and 4,496 controls. The 150 polymorphisms were selected by genome-wide association studies of MI and ischemic stroke with the use of the GeneChip Human Mapping 500K Array Set. The genoty...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Yoshida T, Kato K, Yokoi K, Oguri M, Watanabe S, Metoki N, Yoshida H, Satoh K, Aoyagi Y, Nozawa Y, Yamada Y Tags: Int J Mol Med Source Type: journals

Antitumor activity of asukamycin, a secondary metabolite from the actinomycete bacterium Streptomyces nodosus subspecies asukaensis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Asukamycin, a manumycin-type metabolite, was isolated by a rapid and easily scalable purification scheme. Thus far, studies on the biological activity of asukamycin have been limited to its role as an antibacterial and antifungal agent. By using five different tumor cell lines we demonstrate antineoplastic activity of asukamycin. It inhibited cell growth at concentrations similar to other members of the manumycin family (IC50 1-5 microM). Cytotoxicity of asukamycin was accompanied by activation of caspases 8 and 3 and was diminished by SB 202190, a specific p38 mitogen-activated protein kinase (MAPK) inhibitor. These d...
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Shipley PR, Donnelly CC, Le CH, Bernauer AD, Klegeris A Tags: Int J Mol Med Source Type: journals

JNK and p38 inhibitors increase and decrease apoptosis, respectively, in pyrogallol-treated calf pulmonary arterial endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, PG induced apoptosis via the loss of MMP (DeltaPsi(m)) in CPAEC, which is accompanied by GSH depletion. JNK and p38 inhibitors increased and decreased apoptosis in PG-treated CPAEC, respectively, which were correlated with GSH depletion. PMID: 19787207 [PubMed - in process] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Han YH, Moon HJ, You BR, Kim SZ, Kim SH, Park WH Tags: Int J Mol Med Source Type: journals

Cosmetic textiles with biological benefits: Gelatin microcapsules containing Vitamin C.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study discusses the development of cosmetic textiles and addresses microencapsulation technology with respect to its historical background, significant advantages, microencapsulation methods and recent applications in the textile industry. Gelatin microcapsules containing vitamin C were prepared using emulsion hardening technique. Both the optical microscopy and scanning electron microscopy demonstrated that the newly developed microcapsules were in the form of core-shell spheres with relatively smooth surface. The particle size of microcapsules ranged from 5.0 to 44.1 microm with the average particle size being 24.6 ...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Cheng SY, Yuen MC, Kan CW, Cheuk KK, Chui CH, Lam KH Tags: Int J Mol Med Source Type: journals

Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study reports the identification of human sex hormone binding globulin (SHBG)-interacting proteins in the brain using a phage display-based screening technology. Phage display is a system in which a foreign protein is displayed on the surface of a bacteriophage as a fusion protein with one of the coat proteins of the bacteriophage. T7 phage clones expressing normal human brain proteins (human normal brain phage-display cDNA expression library) were screened using SHBG as bait. The bound phage clones were then identified by DNA sequencing and by BLAST search analysis. Of the twenty binding proteins analyzed,...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Gnanasekar M, Suleman FG, Ramaswamy K, Caldwell JD Tags: Int J Mol Med Source Type: journals

A novel mutated cell line with characteristics of dedifferentiated chondrosarcoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, a novel dedifferentiated CS cell line, MS0812, was spontaneously established from mutated human embryonic muscle cells. Several features of the cell line were investigated, including growth characteristics, cytogenetics, electron microscopic features, expression of various antigenic markers and tumor formation. MS0812 has been cultured continuously for more than 3 years. The growth characteristics of MS0812 are similar to the immortalized cell lines as reported. The cell line exhibited complex karyotypes and hyperploidy, the chromosome number ranged from 50 to 158. MS0812 was positive for vimentin, desmin an...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Yang L, Chen Q, Zhang S, Wang X, Li W, Wen J, Huang X, Zheng J, Huang G, Huang T, Ju G Tags: Int J Mol Med Source Type: journals

In vitro preparation and characterization of the human CD3epsilonepsilon homodimer and CD3epsilongamma and CD3epsilondelta heterodimers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we investigated whether interactions between each CD3epsilon subunit play a role in the formation of the CD3 molecular complex. Our results revealed that the human CD3epsilon subunit forms a homodimer structure, which is a crucial piece of information for the elucidation of cellular signaling following TCR receptor ligation, and provide insight into our understanding of the molecular assembly of the CD3 molecular complex. PMID: 19724882 [PubMed - in process] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Su Z, Wang H, Wan Y, Bi Z Tags: Int J Mol Med Source Type: journals

Notch1 induces enhanced expression of Delta-like-1 in the U251MG glioma cell line.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The Notch signaling pathway takes part in coordinated regulation of cell growth, survival and differentiation. Previous findings have shown that Notch1 and Delta-like-1 (DLL1) are overexpressed in many glioma cell lines and primary human gliomas. Down-regulation of DLL1 by RNA interference inhibits proliferation and induces apoptosis in multiple glioma cell lines. Our studies showed that Notch1 expression plasmid induced more expression of DLL1 in the U251MG glioma cell line. Adversely, blocking Notch1 receptors down-regulated the expression of DLL1. Both down-regulating DLL1 and blocking Notch1 receptors induced U251M...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Qian CF, Yan W, Zhang JX, Shi L, Qian J, Fu Z, Kang CS, Liu N, You YP Tags: Int J Mol Med Source Type: journals

Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we investigated the role of interleukin-15 (IL-15), a cytokine previously known to be involved in the control of fat accretion by adipose cells, in the differentiation of the 3T3-L1 preadipose cell line. We found that IL-15 is able to increase alpha-calcineurin mRNA content in white adipose tissue of rats chronically treated with the cytokine and also in the 3T3-L1 preadipose cell line. Moreover, IL-15 promoted a decrease in both leptin mRNA expression and lipid accumulation, as estimated by Red Oil O staining. Cotreatment with IL-15 and FK506 (a calcineurin inhibitor) resulted in no changes in lipid content ...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Almendro V, Fuster G, Ametller E, Costelli P, Pilla F, Busquets S, Figueras M, Argilés JM, López-Soriano FJ Tags: Int J Mol Med Source Type: journals

Cholesterol overloading leads to hepatic L02 cell damage through activation of the unfolded protein response.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study suggests that cholesterol overloading in hepatic L02 cells induces ERS and activates the UPR which, in part, leads to the apoptotic damage of cells. PMID: 19724885 [PubMed - in process] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Li Q, Liu Z, Guo J, Chen J, Yang P, Tian J, Sun J, Zong Y, Qu S Tags: Int J Mol Med Source Type: journals

Establishment and characterization of the human SaTM-1 anal canal squamous cell carcinoma cell line derived from lymph node metastasis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human anal canal squamous cell carcinoma (SCC) cell line has not yet been reported due to the rarity of this disease. Since cell lines to study this malignancy were not available, we attempted to establish and characterize anal canal SCC cell line from primary culture of lymph node metastasis. Six sublines were cloned and isolated from parental cells. They were designated as SaTM-1A, B, C, D, E and F. The features of the six sublines were characterized by reverse transcription-PCR, chemosensitivity test to 5-Fu and CDDP, immunohistochemistry, cDNA microarray analysis and tumorigenicity using immunodeficient mice. All s...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Takeda A, Maeda M, Iseki H, Hirooka E, Shinozuka N, Koyama I Tags: Int J Mol Med Source Type: journals

Laser capture microdissection: A tool for the molecular characterization of histologic subtypes of lung adenocarcinoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The histologic heterogeneity of lung adenocarcinoma is well known. Many histologic subtypes have been described, and recently their prognostic and predictive value has emerged. Laser capture microdissection may aid in the isolation of cancer cells from distinct subtypes of lung adenocarcinoma, thus enabling the description of their specific molecular features. Characterization of epidermal growth factor receptor (EGFR) mutations in histologic subtypes of lung adenocarcinoma has become an important issue. The purpose of this study was to analyze EGFR mutations in exons 18-21 in single histologic subtypes of lung adenoca...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Donati V, Lupi C, Alì G, Corsi V, Viti A, Lucchi M, Mussi A, Fontanini G Tags: Int J Mol Med Source Type: journals

Low levels of soluble CD1d protein alters NKT cell function in patients with rheumatoid arthritis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study focused on soluble CD1d (sCD1d) by generating recombinant sCD1d proteins and assaying them in plasma using a newly established ELISA method. The amount of sCD1d proteins in plasma was significantly decreased in rheumatoid arthritis (RA) patients (55.2+/-13.3 years, mean +/-SD) compared with healthy donors (31.2+/-7.4 years). Plasma sCD1d protein levels correlated with the number of NKT cells (TCR Valpha24+ Vbeta11+CD3+) in peripheral blood mononuclear cells (r(2)=0.061). Furthermore, sCD1d proteins induced IFN-gamma production from NKT cells, but neither IL-4 nor IL-10. These findings suggest that the low plasma...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Segawa S, Goto D, Yoshiga Y, Hayashi T, Matsumoto I, Ito S, Sumida T Tags: Int J Mol Med Source Type: journals

Evaluation of the effect of glucosamine administration on biomarkers for cartilage and bone metabolism in soccer players.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study, to investigate the effect of glucosamine, a component of glycosaminoglycans with a chondroprotective action, on articular cartilage in athletes, we looked at soccer players, who expose their joints to excessive motion and loading, and compared the levels of biomarkers for type II collagen degradation (CTX-II) and type II collagen synthesis (CPII) between soccer players and non-athlete controls, and in soccer players before and after glucosamine-administration. CTX-II (P<0.01) and CPII (P=0.08) levels were substantially elevated in soccer players compared with those in controls, indicating that ...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Yoshimura M, Sakamoto K, Tsuruta A, Yamamoto T, Ishida K, Yamaguchi H, Nagaoka I Tags: Int J Mol Med Source Type: journals

Induction of G1 arrest and apoptosis by schisandrin C isolated from Schizandra chinensis Baill in human leukemia U937 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We isolated two phytochemical lignans, schisandrin and schisandrin C, from Schizandra chinensis Baill and investigated their anti-cancer effects in human leukemia U937 cells. Schisandrin C inhibited cell growth in a dose-dependent manner, which was associated with the induction of G1 arrest of the cell cycle and apoptosis; schisandrin did not inhibit growth. Schisandrin C induced G1 arrest was correlated with down-regulation of cyclin D1, cyclin E, cyclin-dependent kinase (Cdk) 4 and E2Fs expression, inhibition of phosphorylation of retinoblastoma protein (pRB), and up-regulation of the Cdk inhibitor p21(WAF1/CIP1). In...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Park C, Choi YW, Hyun SK, Kwon HJ, Hwang HJ, Kim GY, Choi BT, Kim BW, Choi IW, Moon SK, Kim WJ, Choi YH Tags: Int J Mol Med Source Type: journals

Effects of local vibrations on skeletal muscle trophism in elderly people: mechanical, cellular, and molecular events.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Several studies have examined the effects of vibrations on muscle mass and performance in young healthy people. We studied the effects of vibrations on muscles of elderly male and female volunteers (65-85 years of age) diagnosed with sarcopenia. We applied mechanical vibrations locally (local vibrational training) to the thigh muscles at 300 Hz for a period of 12 weeks, starting with a session of 15 min stimulation once a week and increasing to three sessions of 15 min per week. Treated muscles displayed enhanced maximal isometric strength and increased content of fast MyHC-2X myosin. Single muscle fiber analysis did n...
Source: International Journal of Molecular Medicine - September 3, 2009 Category: Molecular Biology Authors: Pietrangelo T, Mancinelli R, Toniolo L, Cancellara L, Paoli A, Puglielli C, Iodice P, Doria C, Bosco G, D'Amelio L, di Tano G, Fulle S, Saggini R, Fanò G, Reggiani C Tags: Int J Mol Med Source Type: journals