Genetic diversity reflects geographical origin of < i > Ralstonia solanacearum < /i > strains isolated from plant and water sources in Spain
The characterization and intraspecific diversity of a collection of 45Ralstonia solanacearum strains isolated in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain diversity, phenotypic and genotypic analysis were performed by a polyphasic approach. Biochemical and metabolic profiles were compared. Serological relationship was evaluated by Indirect-ELISA using polyclonal and monoclonal antibodies. For genotypic analysis,hrpB andegl DNA sequence analysis, repetitive sequences (rep-PCR), amplified fragment length polymorphism (AFLP) profiles and macrore...
Source: International Microbiology - March 26, 2018 Category: Microbiology Authors: Paola Caruso, Elena G. Biosca, Edson Bertolini, Ester Marco-Noales, Mar ía Teresa Gorris, Concetta Licciardello, María M. López Source Type: research
Developmental stages identified in the trophozoite of the free-living Alveolate flagellate < i > Colpodella < /i > sp. (Apicomplexa)
In this study we performed light, immunofluorescent and transmission electron microscopy ofColpodella trophozoites to characterize trophozoite morphology and protein distribution. The use of Giemsa staining and antibodies to distinguishColpodella life cycle stages has not been performed previously. Rhoptry andβ-tubulin antibodies were used in immunofluorescent assays (IFA) to identify protein localization and distribution in the trophozoite stage ofColpodella (ATCC 50594). We report novel data identifying“doughnut-shaped” vesicles in the cytoplasm and apical end ofColpodella trophozoites reactive with anti...
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Transmission and survival of carbapenem-resistant < i > Acinetobacter baumannii < /i > outside hospital setting
Acinetobacter baumannii origin and its epidemiology is under a great concern worldwide since this microorganism has become a leading nosocomial pathogen of the 21th century among the " ESKAPE " group of microorganisms. The aim of the study was to monitor and explore the epidemiology of this important hospital pathogen in the second largest clinical university hospital in Croatia. The presence ofA. baumannii in hospital wastewater, as a route for possible transmission outside of the hospital setting, as well as its survival in environmental conditions including seawater, was investigated. During the examination period, ten ...
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Julio R. Villanueva, microbiologist, researcher, and mentor of generations of scientists
(Source: International Microbiology)
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Overview of laboratory methods to diagnose Leptospirosis and to identify and to type leptospires
Leptospirosis is a virulent zoonosis with a global distribution. Pathogenic spirochetes of the genusLeptospiraare responsible for this disease, and the primary animal reservoirs are rodentvvvs. Direct and indirect contact with infected urine constitutes the main route of transmission. Renal failure and advanced abortions are frequently observed in animals affected by leptospirosis, causing serious problems for farms. In humans, there is a high rate of mortality (10 percent), and farmers and persons in contact with water are frequently exposed. However, vaccines and strict prevention measures confer protection against lepto...
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Developmental stages identified in the trophozoite of the free-living Alveolate flagellate Colpodella sp. (Apicomplexa)
In this study we performed light, immunofluorescent and transmission electron microscopy ofColpodella trophozoites to characterize trophozoite morphology and protein distribution. The use of Giemsa staining and antibodies to distinguishColpodella life cycle stages has not been performed previously. Rhoptry andβ-tubulin antibodies were used in immunofluorescent assays (IFA) to identify protein localization and distribution in the trophozoite stage ofColpodella (ATCC 50594). We report novel data identifying“doughnut-shaped” vesicles in the cytoplasm and apical end ofColpodella trophozoites reactive with anti...
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Fate of < i > Bacillus cereus < /i > within phagocytic cells
In this study we assessed the interaction of different strains ofBacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains ofB. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquis...
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Fate of Bacillus cereus within phagocytic cells
In this study we assessed the interaction of different strains ofBacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains ofB. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquis...
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Transmission and survival of carbapenem-resistant Acinetobacter baumannii outside hospital setting
Acinetobacter baumannii origin and its epidemiology is under a great concern worldwide since this microorganism has become a leading nosocomial pathogen of the 21th century among the " ESKAPE " group of microorganisms. The aim of the study was to monitor and explore the epidemiology of this important hospital pathogen in the second largest clinical university hospital in Croatia. The presence ofA. baumannii in hospital wastewater, as a route for possible transmission outside of the hospital setting, as well as its survival in environmental conditions including seawater, was investigated. During the examination period, ten ...
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Genetic diversity reflects geographical origin of < i > Ralstonia solanacearum < /i > strains isolated from plant and water sources in Spain
The characterization and intraspecific diversity of a collection of 45Ralstonia solanacearum strains isolated in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain diversity, phenotypic and genotypic analysis were performed by a polyphasic approach. Biochemical and metabolic profiles were compared. Serological relationship was evaluated by Indirect-ELISA using polyclonal and monoclonal antibodies. For genotypic analysis,hrpB andegl DNA sequence analysis, repetitive sequences (rep-PCR), amplified fragment length polymorphism (AFLP) profiles and macrore...
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain
The characterization and intraspecific diversity of a collection of 45Ralstonia solanacearum strains isolated in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain diversity, phenotypic and genotypic analysis were performed by a polyphasic approach. Biochemical and metabolic profiles were compared. Serological relationship was evaluated by Indirect-ELISA using polyclonal and monoclonal antibodies. For genotypic analysis,hrpB andegl DNA sequence analysis, repetitive sequences (rep-PCR), amplified fragment length polymorphism (AFLP) profiles and macrore...
Source: International Microbiology - March 26, 2018 Category: Microbiology Source Type: research
Overview of laboratory methods to diagnose Leptospirosis and to identify and to type leptospires
Leptospirosis is a virulent zoonosis with a global distribution. Pathogenic spirochetes of the genusLeptospiraare responsible for this disease, and the primary animal reservoirs are rodentvvvs. Direct and indirect contact with infected urine constitutes the main route of transmission. Renal failure and advanced abortions are frequently observed in animals affected by leptospirosis, causing serious problems for farms. In humans, there is a high rate of mortality (10 percent), and farmers and persons in contact with water are frequently exposed. However, vaccines and strict prevention measures confer protection against lepto...
Source: International Microbiology - March 26, 2018 Category: Microbiology Authors: Aur élie Marquez, Zoheira Djelouadji, Virginie Lattard, Angéli Kodjo Source Type: research
Julio R. Villanueva, microbiologist, researcher, and mentor of generations of scientists
(Source: International Microbiology)
Source: International Microbiology - March 26, 2018 Category: Microbiology Authors: C ésar Nombela Source Type: research
Developmental stages identified in the trophozoite of the free-living Alveolate flagellate < i > Colpodella < /i > sp. (Apicomplexa)
In this study we performed light, immunofluorescent and transmission electron microscopy ofColpodella trophozoites to characterize trophozoite morphology and protein distribution. The use of Giemsa staining and antibodies to distinguishColpodella life cycle stages has not been performed previously. Rhoptry andβ-tubulin antibodies were used in immunofluorescent assays (IFA) to identify protein localization and distribution in the trophozoite stage ofColpodella (ATCC 50594). We report novel data identifying“doughnut-shaped” vesicles in the cytoplasm and apical end ofColpodella trophozoites reactive with anti...
Source: International Microbiology - March 26, 2018 Category: Microbiology Authors: Raghavendra Yadavalli, Tobili Y. Sam-Yellowe Source Type: research
Fate of < i > Bacillus cereus < /i > within phagocytic cells
In this study we assessed the interaction of different strains ofBacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains ofB. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquis...
Source: International Microbiology - March 26, 2018 Category: Microbiology Authors: Ivanna S. Rolny, Silvia M. Racedo, Pablo F. P érez Source Type: research
