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815 records returned

Functionalized self-assembling peptide hydrogel enhance maintenance of hepatocyte activity in vitro.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT There is a major challenge in maintaining functional hepatocytes in vivo as these cells rapidly lose their metabolic properties in culture. In this work we have developed a bioengineered platform that replaces the use of the collagen I -in the traditional culture sandwich technique- by a defined extracellular matrix analog, the self-assembling peptide hydrogel RAD16-I functionalized with biologically active motifs. Thus, after examining side by side the two culture systems we have found that in both cases hepatocytes acquired similar parenchymal morphology, presence of functional bile canaliculi structures, CY...
Source: J Cell Mol Med - November 13, 2009 Category: Molecular Biology Authors: Genové E, Schmitmeier S, Sala A, Borrós S, Bader A, Griffith LG, Semino CE Tags: J Cell Mol Med Source Type: journals

Human ribosomal protein S13 promotes gastric cancer growth through downregulating p27(Kip1).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Our previous works revealed that human ribosomal protein S13 (RPS13) was upregulated in multidrug-resistant gastric cancer cells and overexpression of RPS13 could protect gastric cancer cells from drug-induced apoptosis. The present study was designed to explore the role of RPS13 in tumorigenesis and development of gastric cancer. The expression of RPS13 in gastric cancer tissues and normal gastric mucosa was evaluated by immunohistochemical staining and Western blot analysis. It was found RPS13 was expressed at a higher level in gastric cancer tissues than that in normal gastric mucosa. RPS13 was then genetic...
Source: J Cell Mol Med - November 13, 2009 Category: Molecular Biology Authors: Guo X, Shi Y, Gou Y, Li J, Han S, Zhang Y, Huo J, Ning X, Sun L, Chen Y, Sun S, Fan D Tags: J Cell Mol Med Source Type: journals

Bone marrow-derived cells can acquire cardiac stem cells properties in damaged heart.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT Experimental data suggest that cell-based therapies may be useful for cardiac regeneration following ischaemic heart disease. Bone marrow (BM) cells have been reported to contribute to tissue repair after myocardial infarction (MI) by a variety of humoral and cellular mechanisms. However, there is no direct evidence, so far, that BM cells can generate Cardiac Stem Cells (CSCs). To investigate whether BM cells contribute to repopulate the Kit+ CSCs pool, we transplanted BM cells from transgenic mice, expressing Green Fluorescent Protein (GFP) under the control of Kit regulatory elements, into wild type irradiat...
Source: J Cell Mol Med - November 13, 2009 Category: Molecular Biology Authors: Barile L, Cerisoli F, Frati G, Gaetani R, Chimenti I, Forte E, Cassinelli L, Spinardi L, Altomare C, Kizana E, Giacomello A, Messina E, Ottolenghi S, Magli MC Tags: J Cell Mol Med Source Type: journals

The CMT4B disease-causing proteins MTMR2 and MTMR13/SBF2 regulate AKT signaling.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Charcot-Marie-Tooth disease type 4B is caused by mutations in the genes encoding either the lipid phosphatase Myotubularin-Related-Protein-2 (MTMR2) or its regulatory binding partner MTMR13/SBF2. Mtmr2 dephosphorylates PI-3-P and PI-3,5-P2 to form phosphatidylinositol and PI-5-P, respectively, while Mtmr13/Sbf2 is an enzymatically inactive member of the myotubularin protein family. We have found altered levels of the critical signaling protein AKT in mouse mutants for Mtmr2 and Mtmr13/Sbf2. Thus, we analyzed the influence of Mtmr2 and Mtmr13/Sbf2 on signaling processes. We found that overexpression of Mtmr2 pr...
Source: J Cell Mol Med - November 13, 2009 Category: Molecular Biology Authors: Berger P, Tersar K, Ballmer-Hofer K, Suter U Tags: J Cell Mol Med Source Type: journals

PPARgamma ligands inhibit telomerase activity and hTERT expression through modulation of the Myc/Mad/Max network, in colon cancer cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we demonstrated that 15d-PG J2 and rosiglitazone (an endogenous and synthetic PPARgamma ligand, respectively) inhibited hTERT expression and telomerase activity in CaCo-2 colon cancer cells. Moreover, both ligands inhibited c-Myc protein expression and its E-box DNA binding activity. Additionally, Mad1 protein expression and its E-box DNA binding activity were strongly increased by 15d-PG J2 and, to a lesser extent, by rosiglitazone. Sp1 transcription factor expression and its GC-box DNA binding activity were not affected by both PPARgamma ligands. Results obtained by transient transfection of CaCo-2 cells w...
Source: J Cell Mol Med - November 13, 2009 Category: Molecular Biology Authors: Toaldo C, Pizzimenti S, Cerbone A, Pettazzoni P, Menegatti E, Daniela B, Minelli R, Giglioni B, Dianzani MU, Ferretti C, Barrera G Tags: J Cell Mol Med Source Type: journals

Functional alterations of myeloid cell subsets in hyperlipidemia: relevance for atherosclerosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Atherosclerosis is a chronic inflammatory disease wherein the infiltration of myeloid cells of the vessel wall is a hallmark event. Lymphocytes, platelets and endothelial cells stand out as prominent suspects being involved in atherosclerosis. However, recent advances suggest a crucial role for myeloid leukocytes, specifically monocyte subsets, neutrophils, dendritic cells, and endothelial progenitor cells. These cell types are not just rapidly recruited or already reside in the vascular wall, but also initiate and perpetuate core mechanisms in plaque formation and destabilization. Hyperlipidemia is an indepen...
Source: J Cell Mol Med - November 9, 2009 Category: Molecular Biology Authors: Soehnlein O, Drechsler M, Hristov M, Weber C Tags: J Cell Mol Med Source Type: journals

Identification of microRNA profiles in docetaxel-resistant human non-small cell lung carcinoma cells (SPC-A1).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Docetaxel has been used as first-line chemotherapy in advanced NSCLC, but further extensive and effective application is prevented by drug resistance. MicroRNAs (miRNAs) have recently been identified as important posttranscriptional regulators, which are involved in various biological processes. The aim of this study was to identify microRNA expression profiles involved in the development of docetaxel resistance in non-small cell lung carcinoma (NSCLC). Here, microarray chip technology was employed to identify miRNA expression profiles in docetaxel-resistant human NSCLC cell line (SPC-A1/docetaxel). Then, the ...
Source: J Cell Mol Med - November 9, 2009 Category: Molecular Biology Authors: Rui W, Bing F, Hai-Zhu S, Wei D, Long-Bang C Tags: J Cell Mol Med Source Type: journals

Hypoxia-inducible factors in stem cells and cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Cellular properties are influenced by complex factors inherent to their microenvironments. While oxygen deprivation (hypoxia) occurs in tumors due to rapid cell proliferation and aberrant blood vessel formation, embryonic cells develop in a naturally occurring hypoxic environment. Cells respond to hypoxia by stabilizing hypoxia-inducible factors (HIFs), which are traditionally viewed to function by altering cellular metabolism and blood vessel architecture. Recently, HIFs have been shown to modulate specific stem cell effectors, such as Notch, Wnt, and Oct4, that control stem cell proliferation, differentiatio...
Source: J Cell Mol Med - November 9, 2009 Category: Molecular Biology Authors: Mazumdar J, Dondeti V, Simon MC Tags: J Cell Mol Med Source Type: journals

Mutant presenilin-1 deregulated peripheral immunity exacerbates alzheimer-like pathology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Mutations in the presenilin-1 (PS1) gene are independent causes of familial Alzheimer's disease (AD). AD patients have dysregulated immunity, and PS1 mutant mice exhibit abnormal systemic immune responses. To test whether immune function abnormality caused by a mutant human PS1 gene (mhPS1) could modify AD-like pathology, we reconstituted immune systems of AD model mice carrying a mutant human amyloid precursor protein gene (mhAPP; Tg2576 mice) or both mhAPP and mhPS1 genes (PSAPP mice) with allogeneic bone marrow cells. Here, we report a marked reduction in amyloid-beta (Abeta) levels, beta-amyloid plaques, a...
Source: J Cell Mol Med - November 9, 2009 Category: Molecular Biology Authors: Zhu Y, Obregon D, Hou H, Giunta B, Erhart J, Fernandez F, Mori T, Nikolic W, Zhao Y, Morgan D, Town T, Tan J Tags: J Cell Mol Med Source Type: journals

Roles of pathologists in molecular targeted cancer therapy Introduction.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19891708 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - November 4, 2009 Category: Molecular Biology Authors: Osamura RY Tags: J Cell Mol Med Source Type: journals

Autoimmunity against type VII collagen in inflammatory bowel disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Autoimmunity against type VII collagen, an adhesion molecule of the extracellular matrix in epithelial basement membranes, is causing the rare organ-specific epidermolysis bullosa acquisita (EBA). An intriguing association between EBA and inflammatory bowel disease (IBD) has been extensively documented over the last decades, but, due to the very low incidence of EBA, received little attention from physicians involved in the care of patients with IBD. More recently, autoantibodies against type VII collagen have been detected in up to 68% of IBD patients. While these findings suggest that chronic intestinal infl...
Source: J Cell Mol Med - October 31, 2009 Category: Molecular Biology Authors: Hundorfean G, Neurath MF, Sitaru C Tags: J Cell Mol Med Source Type: journals

Isthmin is a novel secreted angiogenesis inhibitor that inhibits tumor growth in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Antiangiogenesis represents a promising therapeutic strategy for the treatment of various malignances. Isthmin is a gene highly expressed in the isthmus of the midbrain-hindbrain organizer in Xenopus with no known functions. It encodes a secreted 60 kDa protein containing a Thrombospondin Type 1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at the C-terminal. In this work, we demonstrate that isthmin is a novel angiogenesis inhibitor. Recombinant mouse isthmin inhibited endothelial cell (EC) capillary network formation on Matrigel through i...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Xiang W, Ke Z, Zhang Y, Cheng GH, Irwan ID, Sulochana KN, Potturi P, Wang Z, Yang H, Wang J, Zhuo L, Kini RM, Ge R Tags: J Cell Mol Med Source Type: journals

Phosphatidylinositol 3-kinase interacts with the glucocorticoid receptor upon TLR2 activation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT Airway inflammation is a common condition where glucocorticoids (GC) are a well-established therapy. It has been demonstrated that GC stimulate components of innate immunity. Specifically, glucocorticoids up-regulate TLR2 expression and activation upon inflammatory stimuli; however, little is known about the signaling involved in this process. To determine the mechanism by which dexamethasone modulates TLR2-induced cytokine production this signaling pathway was monitored in a lung epithelial cell line exposed to the TLR2 synthetic agonist, Pam(3)-Cys-Ser-Lys(4). These experiments demonstrate that phosphatidyli...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Arancibia S, Benítez D, Núñez LE, Jewell CM, Langjhar P, Candia E, Zapata-Torres G, Cidlowski JA, González MJ, Hermoso MA Tags: J Cell Mol Med Source Type: journals

Serially heterotransplanted human prostate tumors as an experimental model.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Preclinical research on prostate cancer (PC) therapies uses several models to represent the human disease accurately. A common model uses patient prostate tumor biopsies to develop a cell line by serially passaging and subsequent implantation, in immunodeficient mice. An alternative model is direct implantation of patient prostate tumor biopsies into immunodeficient mice, followed by serial passage in vivo. The purpose of this review is to compile data from the more than 30 years of human PC serial heterotransplantation research. Serially heterotransplanted tumors are characterized by evaluating the histopatho...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Lopez-Barcons LA Tags: J Cell Mol Med Source Type: journals

Involvement of neuropeptide Y and its Y1 and Y5 receptors in maintaining self-renewal and proliferation of human embryonic stem cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Neuropeptide Y (NPY) and NPY receptors are widely expressed in various organs and cell types and have been shown to have pleiotropic functions. However, their presence or role in human embryonic stem cells (hESCs) remains unknown. We now show that undifferentiated hESCs primarily express NPY and its Y1 and Y5 receptors. Inhibition of NPY signaling using either the selective NPY Y1 or Y5 receptor antagonist reduces the maintenance of self-renewal and proliferation of undifferentiated hESCs. We also provide compelling evidence that exogenous NPY supports the long-term growth of undifferentiated hESCs in the abse...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Son MY, Kim MJ, Yu K, Koo DB, Cho YS Tags: J Cell Mol Med Source Type: journals

Exendin-4 regulates pancreatic ABCA1 transcription via CaMKK/CaMKIV pathway.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Activation of the CaMKK/CaMKIV cascade by exendin-4 stimulated ABCA1 gene transcription, indicating that exendin-4 plays an important role in insulin secretion and cholesterol ester content in pancreatic beta cells. PMID: 19874424 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Li J, Murao K, Imachi H, Masugata H, Iwama H, Tada S, Zhang GX, Kobayashi R, Ishida T, Tokumitsu H Tags: J Cell Mol Med Source Type: journals

Metformin attenuates ovarian cancer cell growth in an AMP- kinase dispensable manner.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT Metformin, the most widely used drug for type 2 diabetes activates AMP-activated protein kinase (AMPK), which regulates cellular energy metabolism. Here, we report that ovarian cell lines VOSE, A2780, CP70, C200, OV202, OVCAR3, SKOV3ip, PE01 and PE04 predominantly express -alpha1, -beta1, -gamma1 and -gamma2 isoforms of AMPK subunits. Our studies show that metformin treatment (1) significantly inhibited proliferation of diverse chemo-responsive and -resistant ovarian cancer cell lines (A2780, CP70, C200, OV202, OVCAR3, SKVO3ip, PE01 and PE04), (2) caused cell cycle arrest accompanied by decreased cyclin D1 and...
Source: J Cell Mol Med - October 29, 2009 Category: Molecular Biology Authors: Rattan R, Giri S, Hartmann L, Shridhar V Tags: J Cell Mol Med Source Type: journals

Dedifferentiation of human articular chondrocytes is associated with alterations in expression patterns of GDF-5 and its receptors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Human articular chondrocytes (HACs) are expanded in monolayer culture in order to obtain sufficient cells for matrix-associated cartilage transplantation (MACT). During this proliferation process, the cells change their shape as well as their expression profile. These changes resemble those that occur during embryogenesis, when the limb anlagen form the interzone that later develops the joint cleft. We analyzed the expression profile of genes that are reportedly important for these changes during embryogenesis within the dedifferentiation process of adult articular chondrocytes. We found GDF-5, BMPR-Ib, and co...
Source: J Cell Mol Med - October 28, 2009 Category: Molecular Biology Authors: Schlegel W, Albrecht C, Eckl P, Freudenthaler H, Berger A, Vécsei V, Marlovits S Tags: J Cell Mol Med Source Type: journals

Sensing, physiological effects and molecular response to elevated CO(2) levels in eukaryotes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Carbon dioxide (CO(2)) is an important gaseous molecule that maintains biosphere homeostasis and is an important cellular signalling molecule in all organisms. The transport of CO(2) through membranes has fundamental roles in most basic aspects of life in both plants and animals. There is a growing interest in understanding how CO(2) is transported into cells, how it is sensed by neurons and other cell types, and in understanding the physiological and molecular consequences of elevated CO(2) levels (hypercapnia) at the cell and organism levels. Human pulmonary diseases and model organisms such as fungi, C. ele...
Source: J Cell Mol Med - October 23, 2009 Category: Molecular Biology Authors: Sharabi K, Lecuona E, Helenius IT, Beitel G, Sznajder JI, Gruenbaum Y Tags: J Cell Mol Med Source Type: journals

Tissue inhibitor of metalloproteinases-1-induced scattered liver metastasis is mediated by host-derived urokinase-type plasminogen activator.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT Paradoxically, not only proteinases but also their inhibitors can correlate with bad prognosis of cancer patients, underlining the evolving concept of the protease web as the complex interplay between proteinases, their inhibitors, and effector molecules. Elevated levels of tissue inhibitor of metalloproteinases-1 (TIMP-1) render the liver more susceptible to metastasis by triggering urokinase plasminogen activator (uPA) expression as well as hepatocyte growth factor (HGF) signalling, thereby leading to the fatal scattered infiltration of metastasizing tumour cells throughout the parenchyma of the target organ...
Source: J Cell Mol Med - October 23, 2009 Category: Molecular Biology Authors: Schrötzlmair F, Kopitz C, Halbgewachs B, Lu F, Algül H, Brünner N, Gänsbacher B, Krüger A Tags: J Cell Mol Med Source Type: journals

Lipid droplet growth by fusion: insights from freeze-fracture imaging.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract An understanding of how lipid droplets grow in the cell is important to current human health issues. Homotypic fusion of small lipid droplets to create larger ones is one proposed mechanism though the evidence for this process continues to be debated. By applying the technique of freeze-fracture electron microscopy to cells that have been stimulated to accumulate lipid droplets, we here present images which suggest that at least some large lipid droplets may indeed result from amalgamation of multiple smaller ones. These visual data add significantly to the notion that fusion contributes to lipid droplet growt...
Source: J Cell Mol Med - October 23, 2009 Category: Molecular Biology Authors: Robenek H, Severs NJ Tags: J Cell Mol Med Source Type: journals

Preservation of TSPO by chronic intermittent hypobaric hypoxia confers antiarrhythmic activity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Abnormal activation of mitochondrial translocator protein (TSPO) contributes to arrhythmogenesis during cardiac metabolic compromise; however, its role in the antiarrhythmic activities of chronic hypoxia adaptation remains unclear. Our results demonstrated that 80% of normoxic rats developed ischemic VF, whereas this condition was seldom observed in rats with 14-days of chronic intermittent hypobaric hypoxia (CIHH). TSPO stimulation or inhibition affected the arrhythmias incidence in normoxic rats, but did not change the CIHH-mediated antiarrhythmic effects. Abrupt and excessive elevation of TSPO activity was ...
Source: J Cell Mol Med - October 23, 2009 Category: Molecular Biology Authors: Li J, Xu J, Xiao J, Zhang H, Liang D, Liu Y, Zhang Y, Liu Y, Wen W, Hu Y, Yu Z, Yan B, Jiang B, Zhou ZN, Chen YH Tags: J Cell Mol Med Source Type: journals

Ox-LDL modifies the behavior of bone marrow stem cells and impairs their endothelial differentiation via inhibition of Akt phosphorylation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, ox-LDL promotes apoptosis and inhibits Oct-4 expression and self-renewal of MAPCs, and impairs their endothelial differentiation via suppression of Akt signaling. PMID: 19863696 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 23, 2009 Category: Molecular Biology Authors: Chu L, Hao H, Luo M, Huang Y, Chen Z, Lu T, Zhao X, Verfaillie CM, Zweier JL, Liu Z Tags: J Cell Mol Med Source Type: journals

Maternal thyroid hormones are transcriptionally active during embryo-fetal development: results from a novel transgenic mouse model.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: our results provide in vivo direct evidence that during embryonic life and before the onset of FTF, maternal THs are transcriptionally active through the action of embryonic TRs. This model may have clinical relevance and may be employed to design endpoint assays for new molecules affecting THs action. PMID: 19863697 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 23, 2009 Category: Molecular Biology Authors: Nucera C, Muzzi P, Tiveron C, Farsetti A, La Regina F, Foglio B, Shih SC, Moretti F, Pietra LD, Mancini F, Sacchi A, Trimarchi F, Vercelli A, Pontecorvi A Tags: J Cell Mol Med Source Type: journals

Native LDL-induced oxidative stress in human proximal 1 tubular cells: multiple players involved.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to examine the effects of native non oxidized LDL on cellular oxidative metabolism in cultured human proximal tubular cells. By means of confocal microscopy imaging combined to respirometric and enzymatic assays it is shown that purified native LDL caused a marked increase of cellular ROS production which was mediated by activation of NADPH oxidase(s) and by mitochondrial dysfunction by means of a ROS-induced ROS release mechanism. The LDL-dependent mitochondrial alterations comprised inhibition of the respiratory chain activity, enhanced ROS production, uncoupling of the oxidative phosphorylation efficien...
Source: J Cell Mol Med - October 23, 2009 Category: Molecular Biology Authors: Piccoli C, Quarato G, D'Aprile A, Montemurno E, Scrima R, Ripoli M, Gomaraschi M, Cirillo P, Boffoli D, Calabresi L, Gesualdo L, Capitanio N Tags: J Cell Mol Med Source Type: journals

Metabolic profiling reveals key metabolic features of renal cell carcinoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Recent evidence suggests that metabolic changes play a pivotal role in the biology of cancer and in particular renal cell carcinoma (RCC). Here, a global metabolite profiling approach was applied to characterize the metabolite pool of RCC and normal renal tissue. Advanced decision tree models were applied to characterize the metabolic signature of RCC and to explore features of metastasized tumors. The findings were validated in a second independent dataset. Vitamin E derivates and metabolites of glucose, fatty acid, and inositol phosphate metabolism determined the metabolic profile of RCC. Alpha-tocopherol, h...
Source: J Cell Mol Med - October 19, 2009 Category: Molecular Biology Authors: Catchpole G, Platzer A, Weikert C, Kempkensteffen C, Johannsen M, Krause H, Jung K, Miller K, Willmitzer L, Selbig J, Weikert S Tags: J Cell Mol Med Source Type: journals

Telomere attrition and genomic instability in xeroderma pigmentosum type-B deficient fibroblasts under oxidative stress.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT Xeroderma Pigmentosum B (XPB/ ERCC3/ p89) is an ATP-dependent 3'-->5' directed DNA helicase involved in basal RNA transcription and the nucleotide excision repair pathway (NER). While the role of NER in alleviating oxidative DNA damage has been acknowledged it remains poorly understood. To study the involvement of XPB in repair of oxidative DNA damage, we utilised primary fibroblasts from a patient suffering from Xeroderma Pigmentosum with Cockayne Syndrome and Hydrogen Peroxide (H(2)O(2)) to induce oxidative stress. Mutant cells retained higher viability and cell cycle dysfunction after H(2)O(2) exposure. ...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Ting AP, Low GK, Gopalakrishnan K, Hande MP Tags: J Cell Mol Med Source Type: journals

Assessment of tumor hypoxia for prediction of response to therapy and cancer prognosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Tumor cells exploit both genetic and adaptive means to survive and proliferate in hypoxic microenvironments, resulting in the outgrowth of more aggressive tumor cell clones. Direct measurements of tumor oxygenation, and surrogate markers of the hypoxic response in tumors (for instance, hypoxia inducible factor-1alpha, carbonic anhydrase 9 and glucose transporter-1) are well-established prognostic markers in solid cancers. However, individual markers do not fully capture the complex, dynamic and heterogeneous hypoxic response in cancer. To overcome this, expression profiling has been employed to identify hypoxi...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Jubb AM, Buffa FM, Harris AL Tags: J Cell Mol Med Source Type: journals

Ankyrin protein networks in membrane formation and stabilization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract In eukaryotic cells, ankyrins serve as adaptor proteins that link membrane proteins to the underlying cytoskeleton. These adaptor proteins form protein complexes consisting of integral membrane proteins, signaling molecules, and cytoskeletal components. With their modular architecture and ability to interact with many proteins, ankyrins organize and stabilize these protein networks, thereby establishing the infrastructure of membrane domains with specialized functions. To this end, ankyrin collaborates with a number of proteins including cytoskeletal proteins, cell adhesion molecules, and large structural prot...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Cunha SR, Mohler PJ Tags: J Cell Mol Med Source Type: journals

Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT Aldehyde dehydrogenases (ALDH) are a family of enzymes that efficiently detoxify aldehydic products generated by reactive oxygen species and might therefore participate in cell survival. Since ALDH activity has been used to identify normal and malignant cells with stem cell properties, we asked whether human myogenic precursor cells (myoblasts) could be identified and isolated based on their levels of ALDH activity. Human muscle explant-derived cells were incubated with ALDEFLUOR, a fluorescent substrate for ALDH, and we determined by flow cytometry the level of enzyme activity. We found that ALDH activity pos...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Jean E, Laoudj-Chenivesse D, Notarnicola C, Rouger K, Serratrice N, Bonnieu A, Gay S, Bacou F, Duret C, Carnac G Tags: J Cell Mol Med Source Type: journals

A systems biology perspective on sVEGFR1: its biological function, pathogenic role & therapeutic use.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A systems biology perspective on sVEGFR1: its biological function, pathogenic role & therapeutic use. J Cell Mol Med. 2009 Oct 16; Authors: Wu FT, Stefanini MO, Gabhann FM, Kontos CD, Annex BH, Popel AS Abstract Angiogenesis is the growth of new capillaries from pre-existent microvasculature. A wide range of pathological conditions, from atherosclerosis to cancer, can be attributed to either excessive or deficient angiogenesis. Central to the physiological regulation of angiogenesis is the vascular endothelial growth factor (VEGF) system - its ligands and receptors (VEGFRs) are thus prime molecular targets ...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Wu FT, Stefanini MO, Gabhann FM, Kontos CD, Annex BH, Popel AS Tags: J Cell Mol Med Source Type: journals

Plasma gelsolin facilitates interaction between beta(2) glycoprotein I and alpha5beta1 integrin.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we identified plasma gelsolin as a protein associated with beta(2)GPI by using immunoaffinity chromatography and mass spectrometric analysis. An in vivo binding assay showed that endogenous beta(2)GPI interacts with plasma gelsolin, which binds to integrin alpha5beta1 through fibronectin. The tethering of beta(2)GPI to monoclonal anti-beta(2)GPI autoantibody on the cell surface was enhanced in the presence of plasma gelsolin. Immunoblot analysis demonstrated that p38 MAPK protein was phosphorylated by monoclonal anti-beta(2)GPI antibody treatment, and its phosphorylation was attenuated in the presence of ant...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Bohgaki M, Matsumoto M, Atsumi T, Kondo T, Yasuda S, Horita T, Nakayama KI, Okumura F, Hatakeyama S, Koike T Tags: J Cell Mol Med Source Type: journals

FHL1 interacts with estrogen receptors and regulates breast cancer cell growth.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Four and a half LIM protein 1 (FHL1) belongs to the LIM-only protein family and plays important roles in muscle growth and carcinogenesis. However, the biological function of FHL1 remains largely unknown. Here, we show that FHL1 physically and functionally interacted with estrogen receptors (ERs), which are involved in breast cancer development and progression. FHL1 bound specifically to the activation function-1 (AF1) domain of ER. Physical interaction of FHL1 and ER is required for FHL1 repression of estrogen-responsive gene transcription. FHL1 affected recruitment of ER to an estrogen-responsive promoter an...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Ding L, Niu C, Zheng Y, Xiong Z, Liu Y, Lin J, Sun H, Huang K, Yang W, Li X, Ye Q Tags: J Cell Mol Med Source Type: journals

Glomerular parietal epithelial cells of adult murine kidney undergo EMT to generate cells with traits of renal progenitors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT Glomerular parietal epithelial cells (GPECs) are known to revert to embryonic phenotype in response to renal injury. However, the mechanism of de-differentiation in GPECs and the underlying cellular processes are not fully understood. In the present study, we show that cultured GPECs of adult murine kidney undergo epithelial-mesenchymal transition (EMT) to generate cells, which express CD24, CD44 and CD29 surface antigens. Characterization by qRT-PCR and immunostaining of these clonogenic cells demonstrate that they exhibit metastable phenotype with co-expression of both epithelial (cytokeratin-18) and mesench...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Chandra V, Phadnis S, Bhonde R Tags: J Cell Mol Med Source Type: journals

Androgen receptor coactivator p44/Mep50 in breast cancer growth and invasion.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report, we examined the expression and function of p44 in breast cancer. In addition to being an AR coactivator, p44 also functions as an ER coactivator. In contrast to findings in prostate cancer, the expression of p44 shows strong cytoplasmic expression in morphologically normal terminal ductal lobular units, while nuclear p44 is observed in both ductal carcinoma in situ (DCIS) and invasive carcinoma. Further, overexpression of nuclear-localized p44 stimulates proliferation and invasion in MCF7 breast cancer cells in the presence of estrogen and is ERalpha-dependent. These findings strongly suggest that p44 plays...
Source: J Cell Mol Med - October 15, 2009 Category: Molecular Biology Authors: Peng Y, Li Y, Gellert LL, Zou X, Wang J, Singh B, Xu R, Chiriboga L, Daniels G, Pan R, Zhang DY, Garabedian M, Schneider R, Wang Z, Lee P Tags: J Cell Mol Med Source Type: journals

Breast Cancer Cell Growth Inhibition by Phenethyl Isothiocyanate is Associated with Downregulation of Estrogen Receptor-alpha36.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The dietary isothiocyanates (ITCs) exhibit strong chemopreventive activities for varieties of neoplasms including breast cancer. However, the molecular mechanisms underlying ITC function in breast cancer cells have not been well established. Here, we found that phenethyl isothiocyanate (PEITC) acted more potently than the "pure" antiestrogen ICI 182,780 to inhibit the growth of estrogen receptor (ER)-positive breast cancer MCF7 and H3396 cells and ER-negative MDA-MB-231 and SK-BR-3 cells. PEITC reduced the steady state levels of ER-alpha and it's novel variant, ER-alpha36 in a dose-and time-dependent manner and inhibit...
Source: J Cell Mol Med - October 14, 2009 Category: Molecular Biology Authors: Kang L, Wang ZY Tags: J Cell Mol Med Source Type: journals

Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumor protection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
s O, Montes-de-Oca-Luna R ABSTRACT The endoplasmic reticulum (ER) is where the Major Histocompatibility Complex (MHC) Class I molecules are loaded with epitopes to cause an immune cellular response. Most of the protein antigens are degraded in the cytoplasm to amino acids and few epitopes reach the ER. Antigen targeting of this organelle by Calreticulin fusion avoids this degradation and enhances the immune response. We constructed a recombinant adenovirus to express the E7 antigen with an ER-targeting signal peptide (SP) plus an ER retention signal (KDEL sequence). In cell-culture experiments we demonstrated that this...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Loera-Arias MJ, Martínez-Pérez AG, Barrera-Hernández A, Ibarra-Obregón ER, González-Saldívar G, Martínez-Ortega JI, Rosas-Taraco A, Villanueva-Olivo A, Esparza-González SC, Villatoro-Hernandez J, Saucedo-Cárden Tags: J Cell Mol Med Source Type: journals

Effect of irreversibly glycated LDL in human vascular smooth muscle cells: Lipid loading, oxidative and inflammatory stress.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, AGE-LDL activates hSMC (increasing CD36, LRP1, RAGE), inducing a pro-oxidant state (activation of NADPHox), lipid accumulation and a pro-inflammatory state (expression of MCP-1). These results may partly explain the contribution of AGE-LDL and hSMC to the accelerated atherosclerosis in diabetes. PMID: 19818091 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Sima AV, Botez GM, Stancu CS, Manea A, Raicu M, Simionescu M Tags: J Cell Mol Med Source Type: journals

BiP, an endoplasmic reticulum chaperone, modulates the development of morphine antinociceptive tolerance.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined knock-in mice expressing a mutant BiP with the retrieval sequence deleted in order to elucidate physiological processes that are sensitive to BiP functions. We tested the thermal antinociceptive effect of morphine in heterozygous mutant BiP mice in a hot plate test. Paw withdrawal latencies before and after a single administration of morphine were not significantly different between the wild-type and mutant BiP mice. Repeated morphine administration caused the development of morphine tolerance in the wild-type mice. The activation of glycogen synthase kinase 3beta (GSK3beta) was associated with morphine toleran...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Dobashi T, Tanabe S, Jin H, Mimura N, Yamamoto T, Nishino T, Aoe T Tags: J Cell Mol Med Source Type: journals

A link between the accumulation of DNA damage and loss of multipotency of human mesenchymal stromal cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Human mesenchymal stromal cells (hMSCs) represent and attractive cell source for clinic applications. Besides being mutipotent, recent clinical trials suggest that they secrete both trophic and immunomodulatory factors, allowing allogenic MSCs to be used in a wider variety of clinical situations. The yield of prospective isolation is however very low, making expansion a required step towards clinical applications. Unfortunately, this leads to a significant decrease in their stemness. To identify the mechanism behind loss of multipotency, hMSCs were expanded until replicative senescence and the concomitant mole...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Alves H, Munoz-Najar U, de Wit J, Renard AJ, Hoeijmakers JH, Sedivy JM, van Blitterswijk C, de Boer J Tags: J Cell Mol Med Source Type: journals

Transport characteristics of a novel peptide platform for CNS therapeutics.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT New and effective therapeutics that cross the blood-brain barrier (BBB) are critically needed for treatment of many brain diseases. We characterize here a novel drug development platform that is broadly applicable for the development of new therapeutics with increased brain penetration. The platform is based on the Angiopep-2 peptide, a sequence derived from ligands that bind to low-density lipoprotein receptor-related protein-1 (LRP-1), a receptor expressed on the BBB. Fluorescent imaging studies of a Cy5.5Angiopep-2 conjugate and immunohistochemical studies of injected Angiopep-2 in mice demonstrated efficie...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Bertrand Y, Currie JC, Demeule M, Régina A, Ché C, Abulrob A, Fatehi D, Sartelet H, Gabathuler R, Castaigne JP, Stanimirovic D, Béliveau R Tags: J Cell Mol Med Source Type: journals

Mechanisms of intermittent hypoxia-induced hypertension.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Exposing rodents to brief episodes of hypoxia mimics the hypoxemia and the cardiovascular and metabolic effects observed in patients with obstructive sleep apnea (OSA), a condition that affects between 5% to 20% of the population. Apart from daytime sleepiness, OSA is associated with a high incidence of systemic and pulmonary hypertension, peripheral vascular disease, stroke and sudden cardiac death. The development of animal models to study sleep apnea has provided convincing evidence that recurrent exposure to intermittent hypoxia has significant vascular and hemodynamic impacts that explain much of the cardiovascula...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: González Bosc LV, Resta T, Walker B, Kanagy NL Tags: J Cell Mol Med Source Type: journals

Platelet microparticle levels: A biomarker of thromboangiitis obliterans (Buerger's disease) exacerbation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19818096 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Darnige L, Helley D, Fischer AM, Emmerich J, Smadja DM, Fiessinger JN Tags: J Cell Mol Med Source Type: journals

Epigenetic modulation of the protein kinase a RIIalpha (PRKAR2A) gene by histone deacetylases 1 & 2 in human smooth muscle cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Epigenetic modulation of the protein kinase a RIIalpha (PRKAR2A) gene by histone deacetylases 1 & 2 in human smooth muscle cells. J Cell Mol Med. 2009 Oct 10; Authors: Karolczak-Bayatti M, Loughney AD, Robson SC, Europe-Finner GN Abstract Recently we reported that the expression of the PKA regulatory subunit RIIalpha is dynamically regulated in human smooth muscle cells of the uterus. We showed that expression levels of mRNA/protein were substantially increased during pregnancy and decreased upon labour, changes that were mirrored by particulate type II PKA activity. This implied an important role for RIIal...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Karolczak-Bayatti M, Loughney AD, Robson SC, Europe-Finner GN Tags: J Cell Mol Med Source Type: journals

Zoledronic acid repolarizes tumor-associated macrophages and inhibits mammary carcinogenesis by targeting the mevalonate pathway.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT It is unknown whether zoledronic acid at clinically relevant doses is active against tumors not located in bone. Mice transgenic for the activated ErbB-2 oncogene were treated with a cumulative number of doses equivalent to that recommended in humans. A significant increase in tumor-free and overall survival was observed in mice treated with zoledronic acid. At clinically compatible concentrations, zoledronic acid modulated the mevalonate pathway and affected protein prenylation in both tumor cells and macrophages. A marked reduction in the number of tumor associated macrophages was paralleled by a significant...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Coscia M, Quaglino E, Iezzi M, Curcio C, Pantaleoni F, Riganti C, Holen I, Mönkkönen H, Boccadoro M, Forni G, Musiani P, Bosia A, Cavallo F, Massaia M Tags: J Cell Mol Med Source Type: journals

Characterization of the expression of HTm4 (MS4A3), a cell cycle regulator, in human peripheral blood cells and normal and malignant tissues.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract HTm4 (MS4A3) is a member of a family of four-transmembrane proteins designated MS4A. MS4A proteins fulfill diverse functions, acting as cell surface signaling molecules and intracellular adapter proteins. Early reports demonstrated that HTm4 is largely restricted to the hematopoietic lineage, and is involved in cell cycle control, via a regulatory interaction with the KAP phosphatase, Cyclin A and CDK2. Here we describe the expression pattern of HTm4 in peripheral blood cells using gene expression microarray technology, and in normal fetal and adult human tissues, as well as adult human cancers, using tissue m...
Source: J Cell Mol Med - October 9, 2009 Category: Molecular Biology Authors: Kutok JL, Yang X, Folkerth R, Adra CN Tags: J Cell Mol Med Source Type: journals

Familial and Racial Determinants of Tumor Suppressor Genes Promoter Hypermethylation in Breast Tissues from Healthy Women.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Gene promoter hypermethylation was commonly found in healthy breast tissues from women without cancer, indicating that these events are frequent and early lesions. Race and family history of cancer increase the likelihood of these early events. PMID: 19799643 [PubMed - as supplied by publisher] (Source: J Cell Mol Med)
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Dumitrescu RG, Marian C, Krishnan SS, Spear SL, Kallakury BV, Perry DJ, Convit JR, Seillier-Moiseiwitsch F, Yang Y, Freudenheim JL, Shields PG Tags: J Cell Mol Med Source Type: journals

Hematopoietic stem cells in spleen have distinct differentiative potential for antigen presenting cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, neonatal spleen showed multilineage hematopoietic reconstitution in mouse chimeras, rather than specific or restricted ability to differentiate into L-DC. However, the representation of individual APC subsets was found to be unequal in chimeras partially reconstituted with donor cells, such that more donor-derived progeny were seen for L-DC than for myeloid and cDC subsets. The ability of HSC in spleen to develop into L-DC was indicated by a strong bias in the subset size of these cells over other splenic APC subsets. This type of evidence supports a model whereby spleen represents an important site for hema...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Tan JK, O'Neill HC Tags: J Cell Mol Med Source Type: journals

Functional characterization of the progestagen-associated endometrial protein gene in human melanoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Utilizing gene microarray profiling of melanoma samples, we have recently identified a novel gene over-expressed in both thick primary and metastatic melanomas. This gene, progestagen-associated endometrial protein (PAEP), has never before been implicated in the oncogenic processes of melanoma, with its true function in oncogenesis and tumor progression relatively unknown. Over-expression of the PAEP gene in freshly procured thick primary and metastatic melanoma samples (58%) and daughter cell lines (77%) is confirmed by quantitative RT-PCR, immunohistochemisty, Western blotting and mass spectrometric analysis...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Ren S, Liu S, Howell PM, Zhang G, Pannell L, Samant R, Shevde-Samant L, Tucker JA, Fodstad O, Riker AI Tags: J Cell Mol Med Source Type: journals

Co-ordinated autophagy with resveratrol and gamma-tocotrienol confers synergetic cardioprotection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study compared two dietary phytochemicals, grape-derived resveratrol and palm oil-derived gamma-tocotrienol, either alone or in combination, on the contribution of autophagy in cardioprotection during ischemia and reperfusion. Sprague Dawley rats weighing between 250-300 gm were randomly assigned to one of the following groups: vehicle, ischemia/reperfusion (I/R), resveratrol+I/R, gamma-tocotrienol+I/R, resveratrol+gamma-tocotrienol+I/R. For resveratrol treatments, the rats were gavaged with resveratrol [2.5 mg/kg] for 15 days while for gamma-tocotrienol experiments the rats were gavaged with gamma-tocotrienol [0.3 mg...
Source: J Cell Mol Med - October 2, 2009 Category: Molecular Biology Authors: Lekli I, Ray D, Mukherjee S, Gurusamy N, Ahsan MK, Juhasz B, Bak I, Tosaki A, Gherghiceanu M, Popescu LM, Das DK Tags: J Cell Mol Med Source Type: journals