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Adapting Cell-Based Assays to the High Throughput Screening Platform: Problems Encountered and Lessons Learned.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present an overview of the problems encountered with the implementation of multiple cell-based assays at the High Throughput Screening Center at Southern Research Institute as well as empirically defined effective solutions to these problems. These include examples of artifacts induced by temperature differences throughout the screening campaign, cell plating conditions including the effect of room temperature incubation on assay consistency, DMSO carry-over, and incubator induced artifacts. PMID: 19492073 [PubMed - as supplied by publisher]
Source: JALA - June 1, 2008 Category: Laboratory Medicine Authors: Maddox CB, Rasmussen L, White EL Tags: JALA Charlottesv Va Source Type: journals

Compound Management for Quantitative High-Throughput Screening.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An efficient and versatile Compound Management operation is essential for the success of all downstream processes in high-throughput screening (HTS) and small molecule lead development. Staff, equipment, and processes need to be not only reliable, but remain flexible and prepared to incorporate paradigm changes. In the present report, we describe a system and associated processes which enable handling of compounds for both screening and follow-up purposes at the NIH Chemical Genomics Center (NCGC), a recently-established HTS and probe development center within the Molecular Libraries Initiative of the NIH Roadmap. Our ...
Source: JALA - April 1, 2008 Category: Laboratory Medicine Authors: Yasgar A, Shinn P, Jadhav A, Auld D, Michael S, Zheng W, Austin CP, Inglese J, Simeonov A Tags: JALA Charlottesv Va Source Type: journals

An instrument for automated purification of nucleic acids from contaminated forensic samples.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present a novel instrument for DNA purification of forensic samples that is capable of highly effective concentration of nucleic acids from soil particulates, fabric, and other complex samples including solid components. The novel concentration process, known as SCODA, is inherently selective for long charged polymers such as DNA, and therefore is able to effectively reject known contaminants. We present an automated sample preparation instrument based on this process, and preliminary results based on mock forensic samples. PMID: 18438455 [PubMed - as supplied by publisher]
Source: JALA - February 1, 2008 Category: Laboratory Medicine Authors: Broemeling DJ, Pel J, Gunn DC, Mai L, Thompson JD, Poon H, Marziali A Tags: JALA Charlottesv Va Source Type: journals

Microfluidic System for Automated Cell-based Assays.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Microfluidic cell culture is a promising technology for applications in the drug screening industry. Key benefits include improved biological function, higher quality cell-based data, reduced reagent consumption, and lower cost. In this work, we demonstrate how a microfluidic cell culture design was adapted to be compatible with the standard 96-well plate format. Key design features include the elimination of tubing and connectors, the ability to maintain long term continuous perfusion cell culture using a passive gravity driven pump, and direct analysis on the outlet wells of the microfluidic plate. A single microflui...
Source: JALA - December 1, 2007 Category: Laboratory Medicine Authors: Lee PJ, Ghorashian N, Gaige TA, Hung PJ Tags: JALA Charlottesv Va Source Type: journals