Journal of Applied Microbiology
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1751 records returned
Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum)
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Conclusions: Kocuria SM1 successfully controlled vibriosis in rainbow trout, and the mode of action reflected stimulation of the host innate immune system.Significance and Impact of the Study: Probiotics can contribute a significant role in fish disease control strategies, and their use may replace some of the inhibitory chemicals currently used in fish farms. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 20, 2009 Category: Microbiology Authors: S.M. Sharifuzzaman, B. Austin Source Type: journals
Molecular intraspecific characterization of Photobacterium damselae ssp. damselae strains affecting cultured marine fish
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Conclusions: In this study, the techniques tested are confirmed as good tools for molecular typing, because they allow discrimination between P. damselae ssp. damselae strains isolated within the same outbreak. In addition, ERIC-PCR and REP-PCR methods were more adequate for rapid typing of P. damselae ssp. damselae than RAPD, allowing the discrimination at strain level.Significance and Impact of the Study: The results, in agreement with previous studies, confirmed the high intraspecific variability among isolated P. damselae ssp. damselae strains at both phenotypic and genetic levels. This suggests the existence of differ...
Source: Journal of Applied Microbiology - November 18, 2009 Category: Microbiology Authors: A. Labella, M. Manchado, M.C. Alonso, D. Castro, J.L. Romalde, J.J. Borrego Source Type: journals
Interaction between phosphorus and biodegradable organic carbon on drinking water biofilm subject to chlorination
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Conclusions: Biodegradable organic carbon was found to be the primary nutrient in regulating biofilm formation in drinking water regardless of the presence of chlorine. It can be therefore concluded that the removal of an easily biodegradable organic carbon is necessary to minimize the biofilm growth potential induced by the intrusion of phosphorus.Significance and Impact of the Study: Phosphorus introduced to drinking water may interact with biodegradable organic carbon, thus leading to measurable impact on the biofilm formation. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 17, 2009 Category: Microbiology Authors: S.-K. Park, J.Y. Hu Source Type: journals
The probiotic properties of Lactobacillus buchneri P2
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Conclusions: The isolated Lact. buchneri P2 showed probiotic properties of cholesterol reduction, acid and bile tolerance and antimicrobial activity and could remove cholesterol via different ways.Significance and Impact of the Study: A new strain of Lact. buchneri P2 with efficient cholesterol-reducing ability was isolated to provide species diversity of lactobacilli for functional dairy products. And the possible mechanism of cholesterol removal by Lact. buchneri was discussed. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 12, 2009 Category: Microbiology Authors: X.Q. Zeng, D.D. Pan, Y.X. Guo Source Type: journals
Comparative studies of microbial populations in the rumen, duodenum, ileum and faeces of lactating dairy cows
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Conclusions: T-RFLP comparisons illustrate significant changes in microbial diversity as digesta passes from one segment to another. Direct counts indicate that microbial numbers are reduced by eight orders of magnitude from the rumen, through the abomasum, and into the duodenum (from c. 1012 to c. 3·6 × 104 cells per ml). Quantitative PCR analyses of rRNA genes indicate that methanogens are present in the duodenum and ileum.Significance and Impact of the Study: The contribution of microbial populations of the small intestine to the nutrition and health of cattle is seldom addressed but warrants further investigation. (S...
Source: Journal of Applied Microbiology - November 11, 2009 Category: Microbiology Authors: J.C. Frey, A.N. Pell, R. Berthiaume, H. Lapierre, S. Lee, J.K. Ha, J.E. Mendell, E.R. Angert Source Type: journals
Genotypic diversity of lactic acid bacteria isolated from African traditional alkaline-fermented foods
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Conclusion: LAB found in African alkaline-fermented foods belong to a range of genera, species and subspecies of bacteria and vary considerably according to raw material.Significance and Impact of the Study: Our study confirms that LAB survive in alkaline fermentations, a first crucial stage in determining their significance and possible value as probiotic bacteria. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 7, 2009 Category: Microbiology Authors: L.I.I. Ouoba, C.A.G. Nyanga-Koumou, C. Parkouda, H. Sawadogo, S.C. Kobawila, S. Keleke, B. Diawara, D. Louembe, J.P. Sutherland Source Type: journals
Biosynthesis of paromamine derivatives in engineered Escherichia coli by heterologous expression
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Conclusions: Escherichia coli system is engineered for heterologous expression of paromamine derivatives of aminoglycoside biosynthesis.Significance and Impact of the Study: This is the first report of heterologous expression of paromamine gene set in E. coli. Hence a new platform is established in E. coli system for the production of paromamine which is useful for the exploration of novel aminoglycosides by combinatorial biosynthesis of 4,5- and 4,6-disubtituted route of DOS-containing aminoglycosides. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 6, 2009 Category: Microbiology Authors: N.P. Kurumbang, K. Liou, J.K. Sohng Source Type: journals
Nisin-induced expression of pediocin in dairy lactic acid bacteria
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Conclusions: The LAB hosts were able to produce enough pediocin to inhibit the growth of L. monocytogenes Scott A; the growth of L. monocytogenes NR30 was effectively inhibited only by the Lact. casei transformants.Significance and Impact of the Study: This is the first time that the NICE system has been used to express the intact pediocin operon in these LAB hosts. This system could allow for the in situ production of pediocin in fermented dairy foods supplemented with nisin to prevent listeria contamination. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 4, 2009 Category: Microbiology Authors: J.A. Renye Jr., G.A. Somkuti Source Type: journals
Relapsing fever – a forgotten disease revealed
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Borrelial relapsing fever was once a major worldwide epidemic disease that made a significant impact on Livingstone during his epic travels through Africa and throughout Europe. Indeed, the term 'relapsing fever' was first used to describe clinical cases of this disease in Edinburgh. During the last century, we have witnessed the demise of the louse-borne infection, largely through improving standards of living resulting in a reduction in body lice, the vector for Borrelia recurrentis [louse-borne relapsing fever (LBRF)]. The tick-borne zoonotic form of the disease persists in endemic foci around the world [tick-borne rela...
Source: Journal of Applied Microbiology - November 4, 2009 Category: Microbiology Authors: S.J. Cutler Source Type: journals
Isolation of lytic bacteriophage against Vibrio harveyi
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Conclusions: Bacteriophage with lytic activity against V. harveyi were isolated from prawn farm samples. Purified phage of the family Siphoviridae had a clear lytic ability and no apparent transducing properties, indicating they are appropriate for phage therapy. Phage resistance is potentially a major constraint to the use of phage therapy in aquaculture as bacteria are not completely eliminated.Significance and Impact of the Study: Phage therapy is emerging as a potential antibacterial agent that can be used to control pathogenic bacteria in aquaculture systems. The development of phage therapy for aquaculture requires i...
Source: Journal of Applied Microbiology - November 2, 2009 Category: Microbiology Authors: C. Crothers-Stomps, L. Høj, D.G. Bourne, M.R. Hall, L. Owens Source Type: journals
Pyrene degradation and copper and zinc uptake by Fusarium solani and Hypocrea lixii isolated from petrol station soil
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Conclusions: These fungal isolates appear to show promise for use in bioremediation of pyrene or related xenobiotics and removal of copper and zinc from wastes contaminated singly or in combination with these substances.Significance and Impact of the Study: Microbial responses to mixed organic and inorganic pollution are seldom considered: this research highlights the abilities of certain fungal strains to interact with both xenobiotics and toxic metals and is relevant to other studies on natural attenuation and bioremediation of polluted sites. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 31, 2009 Category: Microbiology Authors: J.W. Hong, J.Y. Park, G.M. Gadd Source Type: journals
Carotenoids found in Bacillus
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Conclusions: The most commonly found pigments were yellow, orange and pink. Isolates were nearly always members of the Bacillus genus and in most cases were related with known species such as Bacillus marisflavi, Bacillus indicus, Bacillus firmus, Bacillus altitudinis and Bacillus safensis. Three types of carotenoids were found with absorption maxima at 455, 467 and 492 nm, corresponding to the visible colours yellow, orange and pink, respectively. Although the presence of other carotenoids cannot be ruled out, these three predominant carotenoids appear to account for the pigments obtained in most pigmented bacilli, and ou...
Source: Journal of Applied Microbiology - October 31, 2009 Category: Microbiology Authors: R. Khaneja, L. Perez-Fons, S. Fakhry, L. Baccigalupi, S. Steiger, E. To, G. Sandmann, T.C. Dong, E. Ricca, P.D. Fraser, S.M. Cutting Source Type: journals
Microbiological analysis of composts produced on South Carolina poultry farms
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Aims: The purpose of this study was to determine whether the methods used in compost operations of small and medium-sized poultry forms resulted in the production of an amendment free of foodborne pathogens.Methods and Results: Nine compost heaps on five South Carolina poultry farms were surveyed at different stages of the composting process. Compost samples were analysed for coliforms and enriched for Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes. The waste materials and composting practices differed among the surveyed farms. On two farms, new materials were added to heaps that had previously comple...
Source: Journal of Applied Microbiology - October 30, 2009 Category: Microbiology Authors: M.W. Shepherd Jr, P. Liang, X. Jiang, M.P. Doyle, M.C. Erickson Source Type: journals
Microbial production of conjugated γ-linolenic acid from γ-linolenic acid by Lactobacillus plantarum AKU 1009a
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Conclusion: The practical process of CGLA production from [gamma]-linolenic acid using washed cells of Lact. plantarum AKU 1009a was successfully established.Significance and Impact of the Study: We presented the first example of microbial production of CGLA. CGLA produced by the process is valuable for evaluating their physiological and nutritional effects, and chemical characteristics. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 30, 2009 Category: Microbiology Authors: S. Kishino, J. Ogawa, A. Ando, K. Yokozeki, S. Shimizu Source Type: journals
Identification of stress-related proteins in Escherichia coli using the pollutant cis-dichloroethylene
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Conclusion: Seventeen proteins are involved in the stress network for this organism, and YhcN and YchH were shown to be important for the degradation of cis-DCE.Significance and Impact of the Study: Six previously uncharacterized proteins (YchH, YdeI, YgiW, YhcN, YjaA and YodD) were shown to be stress proteins. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 30, 2009 Category: Microbiology Authors: J. Lee, S.R. Hiibel, K.F. Reardon, T.K. Wood Source Type: journals
Hepatitis E virus-based evaluation of a virion concentration method and detection of enteric viruses in environmental samples by multiplex nested RT-PCR
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Conclusions: The study suggests absence of enteric viruses both in the source and in the purified water samples from Pune city, not allowing evaluation of the purification system and documents high prevalence of enteric viruses in river water, posing threat to the community.Significance and Impact of the Study: The rapid, sensitive and relatively inexpensive protocol developed for virological evaluation of water seems extremely useful and should be adapted for evaluating viral contamination of water for human consumption. This will lead to development of adequate control measures thereby reducing disease burden because of ...
Source: Journal of Applied Microbiology - October 30, 2009 Category: Microbiology Authors: V. Verma, V.A. Arankalle Source Type: journals
Vibrio nigripulchritudo monitoring and strain dynamics in shrimp pond sediments
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Conclusions: These results show that the sediments contain V. nigripulchritudo, notably pathogenic strains. Surprisingly, the virulence-associated plasmid pSFn1 found in all V. nigripulchritudo isolated from moribund shrimp appears less frequently in sediments, possibly being useless or even detrimental to its recipient bacteria in this environment.Significance and Impact of the Study: This study confirms the presence of pathogenic V. nigripulchritudo strains in shrimp pond sediment before a mortality outbreak complying with a previous hypothesis that sediments could be the infecting reservoir. After the outbreak, both tot...
Source: Journal of Applied Microbiology - October 28, 2009 Category: Microbiology Authors: E. Walling, E. Vourey, D. Ansquer, B. Beliaeff, C. Goarant Source Type: journals
Features of Saccharomyces cerevisiae as a culture starter for the production of the distilled sugar cane beverage, cachaça in Brazil
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Conclusions: Cachaça produced by select yeast strains exhibits analytical differences. UFLA CA 1162 and UFLA CA 116 S. cerevisiae isolates can be considered the ideal strains for the artisanal production of cachaça in Brazil.Significance and Impact of the Study: The use of select yeast strains can improve the quality and productivity of cachaça production. Our findings are important for the appropriate monitoring of yeast during sugar cane fermentation. In addition, we demonstrate that UFLA CA 116 and UFLA CA 1162, the ideal yeast strains for cachaça production, are maintained at a high population density. The persiste...
Source: Journal of Applied Microbiology - October 27, 2009 Category: Microbiology Authors: C.R. Campos, C.F. Silva, D.R. Dias, L.C. Basso, H.V. Amorim, R.F. Schwan Source Type: journals
Reliability of mCP method for identification of Clostridium perfringens from faecal polluted aquatic environments
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Conclusions: The molecular results indicated all of the presumptive positive isolates were C. perfringens regardless of the source, e.g. sewage influent or environmental water samples. Sequencing revealed that C. perfringens obtained from sewage and the aquatic environment were nearly identical (c. 99·5% similarity).Significance and Impact of the Study: From this study we conclude that the mCP method is a robust approach to enumerate and isolate C. perfringens from aquatic environments that receive diverse sources of faecal pollution. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 26, 2009 Category: Microbiology Authors: S.R. Mueller-Spitz, L.B. Stewart, S.L. McLellan Source Type: journals
Improvement in cell-bound phytase activity of Pichia anomala by permeabilization and applicability of permeabilized cells in soymilk dephytinization
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Conclusions: Whole cell permeabilization of P. anomala using Triton X-100 led to enhancement in cell-bound phytase activity. The viability and integrity of yeast cells were not significantly affected because of permeabilization. The permeabilized P. anomala cells effectively dephytinized soymilk, and the permeabilized cells immobilized in alginate could be reused because of sustained phytase activity.Significance and Impact of the Study: This is the first report on the use of permeabilized yeast cells for mitigating phytate content of soymilk. Alginate entrapment of permeabilized P. anomala allows reuse of cells for soymil...
Source: Journal of Applied Microbiology - October 26, 2009 Category: Microbiology Authors: P. Kaur, T. Satyanarayana Source Type: journals
DNA extraction from bovine faeces: current status and future trends
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The objective is to identify information that can be used to improve the sensitivity of those PCR methods used after direct DNA extraction. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 26, 2009 Category: Microbiology Authors: D. Rapp Source Type: journals
Complete genome sequence of a newly isolated lytic bacteriophage, EFAP-1 of Enterococcus faecalis, and antibacterial activity of its endolysin EFAL-1
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Conclusions: The complete genome sequence of the newly isolated enterococcal lytic phage was analysed, and it was demonstrated that its recombinant endolysin had broad lytic activity against various Gram-positive pathogens.Significance and Impact of the Study: Bacteriophage EFAP-1 and its lytic protein, EFAL-1, can be utilized as potent antimicrobial agents against Enterococcus spp. strains resistant to conventional antibiotics in hospital infections and also as environmental disinfectants to control disease-causing Enterococcus spp. in dairy farms. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 23, 2009 Category: Microbiology Authors: J.S. Son, S.Y. Jun, E.B. Kim, J.E. Park, H.R. Paik, S.J. Yoon, S.H. Kang, Y.-J. Choi Source Type: journals
Antimicrobial-resistant faecal Escherichia coli in wild mammals in central Europe: multiresistant Escherichia coli producing extended-spectrum beta-lactamases in wild boars
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Conclusions: Antibiotic-resistant E. coli occured in populations of wild mammals in various prevalences.Significance and Impact of the Study: Wild mammals are reservoirs of antibiotic-resistant E. coli including ESBL-producing strains which were found in wild boars. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 21, 2009 Category: Microbiology Authors: I. Literak, M. Dolejska, T Radimersky, J. Klimes, M. Friedman, F.M. Aarestrup, H. Hasman, A. Cizek Source Type: journals
Influence of nitrogen fertilization on tropical-grass silage assessed by ensiling process monitoring using chemical and microbial community analyses
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Conclusions: Nitrate content of herbage is an important factor that influences silage quality, and careful fertilization management can facilitate stable and successful fermentation of tropical-grass silage without any pretreatment.Significance and Impact of the Study: The positive effect of nitrate on the ensiling process of tropical-grass was proved by microbial-community analysis. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 20, 2009 Category: Microbiology Authors: T. Namihira, N. Shinzato, H. Akamine, H. Maekawa, T. Matsui Source Type: journals
Identification of different alkane hydroxylase systems in Rhodococcus ruber strain SP2B, an hexane-degrading actinomycete
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Conclusions: Rhodococcus ruber SP2B should prove to be a promising candidate for bioremediation studies of contaminated sites because of its large degradation range of alkanes.Significance and Impact of the Study: This is the first thorough study on R.ruber alkane degradation systems. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 20, 2009 Category: Microbiology Authors: A. Amouric, M. Quéméneur, V. Grossi, P.-P. Liebgott, R. Auria, L. Casalot Source Type: journals
Isolation and characterization of an ether-type polyurethane-degrading micro-organism and analysis of degradation mechanism by Alternaria sp.
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Conclusions: The enzyme(s) from PURDK2 degraded urethane and urea bonds to convert the high molecular weight structure of ether-PUR to small molecules; and then the fungus seems to use the small molecules as an energy source.Significance and Impact of the Study: Ether-PUR[ndash]degrading fungus, strain PURDK2, was isolated, and the urethane- and urea-bonds[ndash]degrading enzymes from strain PURDK2 could contribute to the material recycling of ether-PUR. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 20, 2009 Category: Microbiology Authors: Y. Matsumiya, N. Murata, E. Tanabe, K. Kubota, M. Kubo Source Type: journals
Growth potential of faecal bacteria in simulated psychrophilic/mesophilic zones during composting of organic waste
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Conclusions: Salm. Typhimurium had the potential for growth in psychrophilic/mesophilic (P/M) zones of immature compost material and its growth potential correlated negatively with the maturity of the compost and the temperature within the simulated P/M zone.Significance and Impact of the Study: The risk of pathogen regrowth in P/M zones during organic waste composting further emphasizes the importance of good management practices and of avoiding P/M zones in combination with low compost maturity. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 20, 2009 Category: Microbiology Authors: J. Elving, J.R. Ottoson, B. Vinnerås, A. Albihn Source Type: journals
Inactivation of Escherichia coli by citral
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Aims: The aim was to evaluate (i) the resistance of Escherichia coli BJ4 to citral in a buffer system as a function of citral concentration, treatment medium pH, storage time and initial inoculum size, (ii) the role of the sigma factor RpoS on citral resistance of E. coli, (iii) the role of the cell envelope damage in the mechanism of microbial inactivation by citral and (iiii) possible synergistic effects of mild heat treatment and pulsed electric fields (PEF) treatment combined with citral.Methods and Results: The initial inoculum size greatly affected the efficacy of citral against E. coli cells. Exposure to 200 [mu]l l...
Source: Journal of Applied Microbiology - October 20, 2009 Category: Microbiology Authors: M. Somolinos, D. García, S. Condón, B. Mackey, R. Pagán Source Type: journals
Simultaneous differential detection of human pathogenic and nonpathogenic Vibrio species using a multiplex PCR based on gyrB and pntA genes
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Conclusions: The multiplex PCR based on combined gyrB and pntA provides a high discriminatory power in the differentiation between Vibrio alginolyticus and V. parahaemolyticus, and between V. cholerae and Vibrio mimicus.Significance and Impact of the Study: This assay will be useful for rapid differentiation of various Vibrio species from clinical and environmental sources and significantly overcomes the limitations of the conventional methods. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 20, 2009 Category: Microbiology Authors: C.S.J. Teh, K.H. Chua, K.L. Thong Source Type: journals
Polyhexamethylene biguanide exposure leads to viral aggregation
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Conclusions: Inactivation kinetics and change in virus hydrophobicity suggested that PHMB induces the formation of viral aggregates. This hypothesis was supported using dynamic light scattering that showed an increase in viral aggregates sizes (up to 500 nm) in a concentration-dependent manner.Significance and Impact of the Study: It has been reported that viral aggregation is responsible for virus survival to the biocide exposure. Here, this might be the case, because the virucidal activity of the biguanides was modest and viral aggregation important. The formation of viral aggregates during virus exposure to PHMB was unl...
Source: Journal of Applied Microbiology - October 20, 2009 Category: Microbiology Authors: F. Pinto, J.-Y. Maillard, S.P. Denyer, P. McGeechan Source Type: journals
Antimicrobial efficiency of titanium dioxide-coated surfaces
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Conclusions: Antimicrobial surfaces and coatings based on titanium dioxide have the potential to effectively inactivate vegetative micro-organisms.Significance and impact of the study: Knowledge about the antimicrobial efficiency of titanium dioxide was gained. This is a prerequisite for industrial applications to improve hygiene, food quality and safety. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 20, 2009 Category: Microbiology Authors: P. Muranyi, C. Schraml, J. Wunderlich Source Type: journals
Production of enterocin A by Enterococcus faecium MMRA isolated from 'Rayeb', a traditional Tunisian dairy beverage
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Conclusions: The inhibitory activity of Ent. faecium MMRA, a wild strain isolated from the artisan dairy beverage 'Rayeb', is due to the synthesis of an enterocin A.Significance and Impact of the Study: Traditional fresh Tunisian fermented dairy products are generally manufactured with raw milk that can be used as a source of uncharacterized wild lactic acid bacteria strains. To our knowledge, this is the first report on the isolation of an enterocin A producing Ent. faecium from 'Rayeb'. This bacteriocin or the producing strain might have a promising potential in biopreservation to enhance the hygienic quality of this dai...
Source: Journal of Applied Microbiology - October 18, 2009 Category: Microbiology Authors: A. Rehaiem, B. Martínez, M. Manai, A. Rodríguez Source Type: journals
Isolation, identification and characterization of soil microbes which degrade phenolic allelochemicals
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Conclusions: Phenolics in plant culture solutions and soils can be decomposed through application of soil microbes in laboratory or controlled conditions. However, modification of growth conditions is more important for acidic and ions-contaminated media.Significance and Impact of Study: The four microbes were first isolated and characterized from the soils of bamboo, rice or pine. This study provides some evidence and methods for microbial control of phenolic allelochemicals. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 15, 2009 Category: Microbiology Authors: Z.-Y. Zhang, L.-P. Pan, H.-H. Li Source Type: journals
Evidence of the co-circulation of enteric viruses in sewage and in the population of Greater Cairo
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Conclusions: The comparison of environmental and clinical data suggests that similar RV and NoV isolates were circulating in the environment and in the population during the same period.Significance and Impact of the Study: Few studies have investigated the prevalence and the epidemiology of RVs and NoVs in Cairo. This work is the first to establish a correlation between viral gastroenteritis and the concomitant presence of enteric viruses in the environment for Greater Cairo where combined environmental and clinical surveys should help to prevent infections caused by these major pathogens. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 14, 2009 Category: Microbiology Authors: A.H. Kamel, M.A. Ali, H.G. El-Nady, S. Aho, P. Pothier, G. Belliot Source Type: journals
Evaluation of new chromogenic substrates for the detection of coliforms
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Conclusions: The range of substrates described can be used in broths as well as in agars. The VQE agar allows the detection of coliforms within a working day. VQE-gal medium proved to be more sensitive when compared to other available chromogenic media and allows the unambiguous detection of coliforms. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 14, 2009 Category: Microbiology Authors: N.K. Browne, Z. Huang, M. Dockrell, P. Hashmi, R.G. Price Source Type: journals
Persistence of enterohaemorrhagic and nonpathogenic E. coli on spinach leaves and in rhizosphere soil*
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Conclusions: Escherichia coli O157:H7 persisted in soil for at least 28 days. Escherichia coli O157:H7 on spinach leaves survived for less than 14 days when co-inoculated with nonpathogenic E. coli. There was no correlation between HPC and E. coli O157:H7 or nonpathogenic E. coli.Significance and Impact of the Study: The persistence of nonpathogenic E. coli isolates makes them possible candidates as surrogates for E. coli O157:H7 on spinach leaves in field trials. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 12, 2009 Category: Microbiology Authors: J. Patel, P. Millner, X. Nou, M. Sharma Source Type: journals
Assessment of survival of Listeria monocytogenes, Salmonella Infantis and Enterococcus faecalis artificially inoculated into experimental waste or compost
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Conclusions: Strain survival was not observed in stabilized composts. The cooling phase seems to be the turning point for pathogen survival and at this stage the indigenous microflora appeared to play a significant role in suppression.Significance and Impact of the Study: The importance of indigenous microflora in the survival of pathogens in four different composts was demonstrated. Stabilized composts were recommended for spreading on land. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 12, 2009 Category: Microbiology Authors: N. Paniel, S. Rousseaux, P. Gourland, M. Poitrenaud, J. Guzzo Source Type: journals
Frequent occurrence of multidrug-resistant CC17 Enterococcus faecium among clinical isolates in Sweden
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Conclusions: The present study demonstrates that nosocomial infections caused by Ent. faecium CC17 are commonly occurring in Sweden.Significance and Impact of the Study: This is the first report of CC17 Ent. faecium in Sweden. The increase of antibiotic resistance and virulence indicates that these strains are further adapting to the hospital environment. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 12, 2009 Category: Microbiology Authors: H. Billström, J. Top, C. Edlund, B. Lund Source Type: journals
Procurement of spore-free Bacillus anthracis for molecular typing outside of BSL3 environment
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Conclusions: This method eliminated viable spores in cell preparations of B. anthracis, yet still allowed the growth of vegetative cells to provide sufficient DNA suitable for analysis by Riboprinter® and PFGE.Significance and Impact of the Study: This method will provide safe cell preparations, prevent instrument contamination, and may be useful for other aerobic and anaerobic spore-formers. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 12, 2009 Category: Microbiology Authors: D.S. King, V.A. Luna, A.C. Cannons, P.T. Amuso Source Type: journals
Esterase as an enzymatic signature of Geodermatophilaceae adaptability to Sahara desert stones and monuments
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Conclusions: Geodermatophilaceae isolates share a rich and particular pool of esterase activities that could be directly linked to harsh conditions characterizing their ecological habitat including high level of aridity, temperature, ionic strength and low nutrient availability.Significance and Impact of the Study: Esterase could be considered as enzymatic signature that outlines adaptability of Geodermatophilaceae in arid area. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 10, 2009 Category: Microbiology Authors: I. Essoussi, F. Ghodhbane-Gtari, H. Amairi, H. Sghaier, A. Jaouani, L. Brusetti, D. Daffonchio, A. Boudabous, M. Gtari Source Type: journals
Purification and identification of antibacterial phenolics from Tripodanthus acutifolius leaves
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Conclusions: The antibacterial activity of T. acutifolius infusion would be partially attributed to the purified glycoflavonoids and mainly to tripodantoside.Significance and Impact: The high extraction yield and the antibacterial activity exhibited by tripodantoside makes this chemical structure of interest to support further studies dealing with chemical modifications to increase the antibacterial activity or to seek another activities. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 7, 2009 Category: Microbiology Authors: J.R. Soberón, M.A. Sgariglia, D.A. Sampietro, E.N. Quiroga, M.G. Sierra, M.A. Vattuone Source Type: journals
In vivo31P and 13C NMR investigations of Rhodococcus rhodochrous metabolism and behaviour during biotransformation processes
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Conclusions: Rhodococcus rhodochrous carbon and energy metabolism is well adapted to different stresses and consequently to live in the environment where conditions are constantly changing.Significance and Impact of the Study: The results of this study can be used to understand the behaviour of this bacterium in natural environments but also in water treatment plants where iron and UV light are present. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 7, 2009 Category: Microbiology Authors: C. Chorao, M. Traïkia, P. Besse-Hoggan, M. Sancelme, R. Bligny, E. Gout, G. Mailhot, A.-M. Delort Source Type: journals
Copper-resistant halophilic bacterium isolated from the polluted Maruit Lake, Egypt
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Conclusions: The highest cell viability was found in the presence of 1·0 mol l[minus]1 NaCl at different copper concentrations, and copper uptake was optimal in the absence of NaCl. Plasmid pMA21 encoded chromate, cobalt, zinc and cadmium transporters, whereas pMA22 encoded specific zinc and RND (resistance, nodulation, cell division) efflux transporters as well as different kinds of metabolic enzymes. Copper resistance was mainly incorporated in the chromosome.Significance and Impact of the Study: Strain MA2 is a fast and efficient tool for copper bioremediation and the isolated plasmids show significant characteristics ...
Source: Journal of Applied Microbiology - October 6, 2009 Category: Microbiology Authors: O. Osman, H. Tanguichi, K. Ikeda, P. Park, S. Tanabe-Hosoi, S. Nagata Source Type: journals
Pathogenic potential and horizontal gene transfer in ovine gastrointestinal Escherichia coli
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Conclusions: The sheep carried Stx phage susceptible E. coli that possessed virulence markers associated with human pathogenicity. Changes in bacterial genomes by phage transfer may complicate outbreak source investigations. Serotype has to be taken into account when evaluating strain relationships by MLVA.Significance and Impact of the Study: Sheep carry E. coli that encode for virulence markers and belong to serogroups known to be human pathogens. In addition, a selection of isolates was found to be susceptible to horizontal transfer of Shiga toxin genes by means of bacteriophages in vitro, and the transfer resulted in a...
Source: Journal of Applied Microbiology - October 6, 2009 Category: Microbiology Authors: D. Döpfer, C. Sekse, L. Beutin, H. Solheim, F.J. van der Wal, A. de Boer, J.S. Slettemeås, Y. Wasteson, A.M. Urdahl Source Type: journals
New protocols for the extraction of nucleic acids from soil
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(Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 6, 2009 Category: Microbiology Authors: D. Per[scaron]oh, G. Rambold Source Type: journals
New protocols for the extraction of nucleic acids from soil – a response to Peršoh and Rambold's letter
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(Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 6, 2009 Category: Microbiology Authors: Y. Wang, S. Morimoto, N. Ogawa, T. Oomori, T. Fujii Source Type: journals
Antimicrobial activity of honey from the stingless bee Trigona carbonaria determined by agar diffusion, agar dilution, broth microdilution and time-kill methodology
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Aims: The aim of this study was to determine the spectrum of antimicrobial activity of 11 samples of stingless bee honey compared to medicinal, table and artificial honeys.Methods and Results: Activity was assessed by agar diffusion, agar dilution, broth microdilution and time-kill viability assays. By agar dilution, minimum inhibitory concentration (MIC) ranges were 4% to >10% (w/v) for Gram-positive bacteria, 6% to >16% (w/v) for Gram-negative bacteria and 6% to >10% (w/v) for Candida spp. By broth microdilution, all organisms with the exception of Candida albicans and Candida glabrata were inhibited at [le]32% (w/v). Ge...
Source: Journal of Applied Microbiology - October 6, 2009 Category: Microbiology Authors: K.L. Boorn, Y.-Y. Khor, E. Sweetman, F. Tan, T.A. Heard, K.A. Hammer Source Type: journals
Development of a quantitative Real-Time TaqMan PCR assay for determination of the minimal dose of Mycoplasma hyopneumoniae strain 116 required to induce pneumonia in SPF pigs
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Conclusion: The triplex RT-PCR test was validated and can be used for testing samples taken on the pig farms.Significance and Impact of the Study: This test should be a very useful tool in pig herds to control enzootic pneumonia or healthy carrier pigs and to study the dynamics of Myc. hyopneumoniae infections. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 6, 2009 Category: Microbiology Authors: C. Marois, D. Dory, C. Fablet, F. Madec, M. Kobisch Source Type: journals
Fungal microbiota dynamics as a postmortem investigation tool: focus on Aspergillus, Penicillium and Candida species
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Conclusions: Aspergillus, Penicillium and Candida species were associated with decomposed human cadavers.Significance and Impact of the Study: These findings enable tracing out the profile of fungal communities of human cadavers for the first time. However, much more research will be necessary to develop this new segment of mycology and to enable its routine use in forensic science. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - October 6, 2009 Category: Microbiology Authors: J.J.C. Sidrim, R.E. Moreira Filho, R.A. Cordeiro, M.F.G Rocha, E.P. Caetano, A.J. Monteiro, R.S.N. Brilhante Source Type: journals
Utilization of oligo- and polysaccharides at microgram-per-litre levels in freshwater by Flavobacterium johnsoniae
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Conclusions: Strain A3 utilizes oligo- and polysaccharides at microgram-per-litre levels. In surface water and in tap water, the organism was able to utilize compounds that were not utilized by strain P17. These compounds may include oligo- and/or polysaccharides.Significance and Impact of the Study: Phytoplanktonic and bacterial polysaccharides can constitute an important biodegradable fraction of natural organic matter in water and may promote growth of heterotrophic bacteria during water treatment and drinking water distribution. Strain A3 can be used to quantify a group of compounds that includes oligo- and polysacchar...
Source: Journal of Applied Microbiology - October 4, 2009 Category: Microbiology Authors: E.L.W. Sack, P.W.J.J. van der Wielen, D. van der Kooij Source Type: journals
