Interactions of organic acids with vancomycin ‐resistant Enterococcus faecium isolated from community wastewater in Texas
ConclusionsVancomycin ‐resistantEnterococcus faecium inhibition correlated with the dissociated OA species. A small decrease in the concentration of the dissociated OAs from optimum may result in allowing VRE strains to escape disinfection.Significance and Impact of the StudyWhen an OA is used to disinfect VRE strains, the concentration of the dissociated OA should be carefully controlled. A concentration of at least 20  mmol l−1 dissociated OA should be maintained when disinfecting VRE. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 26, 2018 Category: Microbiology Authors: R.C. Beier, R.B. Harvey, T.L. Poole, M.E. Hume, T.L. Crippen, L.D. Highfield, W.Q. Alali, K. Andrews, R.C. Anderson, D.J. Nisbet Tags: Original Article Source Type: research

In vitro removal of polycyclic aromatic hydrocarbons by lactic acid bacteria
ConclusionsThe removal of PAHs was significantly related to pH of media, strains of bacteria, type and concentration of PAHsSignificance and Impact of the StudyThis study has been focused on the reduction of polycyclic aromatic hydrocarbons using LABs and probiotics. Our results showed that not only live strains but also inactivated tested strains are able to remove PAHs from aqueous media, presenting new methods to diminish the amount of these contaminants in foods. Furthermore, the results of this study can be used in future research on evaluating the effects of oral administration of probiotic supplements and even dead ...
Source: Journal of Applied Microbiology - November 26, 2018 Category: Microbiology Authors: Mojtaba Yousefi, Nabi Shariatifar, Maryam Tajabadi Ebrahimi, Amir Mohammad Mortazavian, Abdorreza Mohammadi, Nasim Khorshidian, Masoumeh Arab, Hedayat Hosseini Tags: Original Article Source Type: research

Bacterioruberin extracts from a genetically modified hyperpigmented Haloferax volcanii strain: Antioxidant activity and bioactive properties on sperm cells
ConclusionsHVLON3 is an excellent source of natural microbial C50 carotenoids with applicability in Biotechnology, Biomedical and Veterinary fields. HVLON3 Bctr ‐extract improves the quality of cryopreserved ram sperm cells and could be applied to increase insemination yields.Significance and Impact of the StudyThis study provides insight on the bioactive properties of a bioproduct derived from haloarchaea (carotenoids) which are so far underexploited.This article is protected by copyright. All rights reserved. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 25, 2018 Category: Microbiology Authors: Lucia Zalazar, Pablo Pagola, Mar ía Victoria Miró, María Sandra Churio, Micaela Cerletti, Celeste Martínez, María Iniesta‐Cuerda, Ana Josefa Soler Valls, Andreina Cesari, Rosana De Castro Tags: Original Article Source Type: research

In vitro potentiation of carbapenems with tannic acid against carbapenemase ‐producing enterobacteriaceae: exploring natural products as potential carbapenemase inhibitors
ConclusionTA inhibited both MBLs and SBLs by targeting their hydrophobic sites. Moreover, TA had a stronger binding affinity for MBLs than SBLs as the MBLs, specifically VIM ‐1 (−43·7220 ± 0·4513 kcal mol−1) and NDM ‐1(−44·2329 ± 0·3806 kcal mol−1), interact with a larger number of their catalytic active ‐site residues than that of OXA‐48 (−22·5275 ±  0·1300 kcal mol−1) and KPC ‐2 (−22·1164 ± 0·0111 kcal mol−1).Significance and Impact of the StudyTannic acid or its analogues could be  developed into carbapenemase‐inhibiting adjuvants to restore carbapenem activity i...
Source: Journal of Applied Microbiology - November 23, 2018 Category: Microbiology Authors: A.M. Somboro, J. Osei Sekyere, D.G. Amoako, H.M. Kumalo, R. Khan, L.A. Bester, S.Y. Essack Tags: Original Article Source Type: research

Specific egg yolk antibodies (IgY) confer protection against Acinetobacter baumannii in a murine pneumonia model
ConclusionAll the specific IgY antibodies could protect mice against pneumonia caused byA. baumannii.Significance and Impact of the StudyThe specific IgY antibodies could be employed as a pharmaceutical against pneumonia caused byA. baumannii. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 22, 2018 Category: Microbiology Authors: A. Jahangiri, P. Owlia, I. Rasooli, J. Salimian, E. Derakhshanifar, A. Naghipour Erami, E. Darzi Eslam, S. Darvish Alipour Astaneh Tags: Original Article Source Type: research

Key amino acid residues of the AGT1 permease required for maltotriose consumption and fermentation by Saccharomyces cerevisiae
ConclusionsBased on the results obtained with mutant proteins, molecular docking and the localization of amino acid residues, we propose a transport mechanism for theAGT1 permease.Significance and Impact of the StudyOur results present new insights into the structural basis for activeα–glucoside‐H+ symport activity by yeast transporters, providing the molecular bases for improving the catalytic properties of this type of sugar transporters.This article is protected by copyright. All rights reserved. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 22, 2018 Category: Microbiology Authors: D ébora Trichez, Marilia M. Knychala, Catarina M. Figueiredo, Sérgio L. Alves, Marcia A. da Silva, Luiz C. Miletti, Pedro S. de Araujo, Boris U. Stambuk Tags: Original Article Source Type: research

The cellular and molecular mechanism of glutaraldehyde ‐didecyldimethylammonium bromide as a disinfectant against Candida albicans
ConclusionsGlutaraldehyde ‐didecyldimethylammonium bromide disinfectedC. albicans through distorting cell membrane integrity and permeability, disturbing the intracellular homeostasis by intracellular substances leakage, especially K+, Mg2+, ATP and protein, causing electrolyte imbalance of mitochondria, changing DNA structure, which finally led to cell death.Significance and Impact of the StudyThis study focused on the cellular and molecular mechanism of GD as a disinfectant againstC. albicans. It is important to provide theoretical support to GD againstCandida albicans in practical application. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 21, 2018 Category: Microbiology Authors: W. Lin, D. Yuan, Z. Deng, B. Niu, Q. Chen Tags: Original Article Source Type: research

Challenges and opportunities related to the use of chitosan as a food preservative
SummaryChitosan has attracted a growing attention as a food preservative due to its versatility, nontoxicity, biodegradability and biocompatibility. This review aims to provide a critical appraisal of the limitations and opportunities of the use of chitosan as a food preservative. The application of chitosan as a food preservative necessitates insights into mechanisms of chitosan ‐mediated cell death and injury, factors affecting chitosan activity and effects of chitosan on food safety and quality. Chitosan exerts antimicrobial activity by perturbing the negatively charged cell envelope of micro‐organisms with its poly...
Source: Journal of Applied Microbiology - November 20, 2018 Category: Microbiology Authors: Ziyi Hu, Michael G. G änzle Tags: Review Article Source Type: research

Relation between broiler and human Campylobacter jejuni strains isolated in Belgium from 2011 to 2013
ConclusionsIn several cases, theC. jejuni strains isolated from the consumed broiler meat and from the campylobacteriosis case had the same profile, according to the used typing methods. The circulatingCampylobacter strains appear to have remained the same from 2011 till 2013 in Belgium.Significance and Impact of the StudyThis study corroborates previously published data from Belgium that suggest a strong correlation betweenC. jejuni strains isolated from broiler meat and from campylobacteriosis patients. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 20, 2018 Category: Microbiology Authors: A. Duarte, N. Botteldoorn, W.G. Miller, W. Coucke, D. Martiny, M. Hallin, T. Seliwiorstow, L. De Zutter, M. Uyttendaele, O. Vandenberg, K. Dierick Tags: Original Article Source Type: research

A nonconventional two ‐stage fermentation system for the production of aerial conidia of entomopathogenic fungi utilizing surface tension
ConclusionsThe two ‐stage fermentation process here described, based on a simple nonabsorbent inert support, has potential for the application in the production of aerial conidia ofB. bassiana and other fungi.Significance and Impact of the StudyAerial conidia are the most extensive propagule type used in commercial mycopesticides, traditionally produced by solid ‐state fermentation (SSF). The industrial applications and other important benefits of the two‐stage fermentation process here described may overcome some hurdles inherent to SSF aiming for the production of aerial conidia. Additionally, production consistenc...
Source: Journal of Applied Microbiology - November 20, 2018 Category: Microbiology Authors: R.B. Lopes, M. Faria, T.R. Glare Tags: Original Article Source Type: research

Development of a novel methanotrophic process with the helper micro ‐organism Hyphomicrobium sp. NM3
ConclusionsHyphomicrobium sp. NM3 is a promising helper micro ‐organism for methanotrophs.Hyphomicrobium‐methanotroph consortia used concurrently with existing methods can produce an efficient and stable methane oxidation system.Significance and Impact of the StudyThis novel methanotrophic process is superior to those previously reported in the literature, and can provide efficient and stable methane oxidation. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 20, 2018 Category: Microbiology Authors: S. ‐Y. Jeong, T.G. Kim Tags: Original Article Source Type: research

Screening of bacterial isolates related to olive orchard pests in Tunisia using 16S ribosomal RNA and evaluation of their biotechnological potential
ConclusionThe selected species from olive orchards represent a broad ‐spectrum of antimicrobial and insecticidal activities and can be considered promising resources in biological control.Significance And Impact Of StudyBacteria isolated from olive pests in olive orchards were investigated for detecting their potential biotechnological applications.This article is protected by copyright. All rights reserved. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 19, 2018 Category: Microbiology Authors: H Gharsallah, I Ksentini, N Abdelhedi, S Naayma, K Hadj Taieb, M Sahnoun, M. A Triki, M Ksantini, A Leclerque Tags: Original Article Source Type: research

Issue Information
Journal of Applied Microbiology, Volume 125, Issue 6, Page 1569-1569, December 2018. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 15, 2018 Category: Microbiology Tags: Issue Information Source Type: research

Added diagnostic value of  broad‐range 16S PCR on periprosthetic tissue and clinical specimens from other normally sterile body sites
ConclusionsOverall, the yield with 16S PCR was low. For cohort ‐1 16S PCR detected pathogens in 4.3% of culture‐negative specimens, where this was 8.2% for cohort‐2.Significance and Impact of StudyAlthough direct 16S PCR cannot replace culture, it may offer a valuable additional diagnostic option for detection of difficult to culture microorganisms in culture negative clinical specimens.This article is protected by copyright. All rights reserved. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 15, 2018 Category: Microbiology Authors: Anne Q. Reuwer, Wouter van den Bijllaardt, Jean ‐Luc Murk, Anton G. M. Buiting, Jaco J. Verweij Tags: Original Article Source Type: research

Impact of Triclosan Adaptation on Membrane Properties, Efflux and Antimicrobial Resistance of Escherichia coli Clinical Isolates
ConclusionThis study suggests that the extensive use of TCS at sub lethal concentrations contributed to the emergence of antibiotic resistance inE. coli clinical isolates, by inducing changes in bacterial membrane properties and enhancing the efflux system.Significance and Impact of the StudyThe extensive usage of TCS has deleterious effect on the spread of antibiotic resistance, and more studies are needed to investigate the molecular mechanisms underlying the effects of TCS.This article is protected by copyright. All rights reserved. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - November 15, 2018 Category: Microbiology Authors: Fatma Ibrahim Sonbol, Tarek El ‐Said El‐Banna, Ahmed Ahmed Abd El‐Aziz, Engy El‐Ekhnawy Tags: Original Article Source Type: research