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Catalase and superoxide dismutase double staining zymogram technique for Deinococcus and Kocuria species exposed to multiple stressesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to achieve a simple and rapid technique for the double staining of bacterial SOD and catalase on the same polyacrylamide gel. SOD detection was carried out using nitro-blue tetrazolium (NBT) dye reduction followed by ferricyanide precipitation for negative staining of the catalase enzyme on the same gel. The staining procedure resulted in pale blue SOD bands while catalase appeared as yellow bands against a greenish blue background on the same gel. This technique was used to detect changes in the polymorphic forms of these enzymes in Deinococcus radiodurans R1 and Kocuria sp. C2 subjected to...
Source: Journal of Basic Microbiology - October 6, 2009 Category: Microbiology Authors: Manish R Shukla, Radhika Yadav, Anjana Desai Source Type: journals

2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicansEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study found that 2-dodecanol is able to inhibit hyphal development and block SIR2 up-regulation, even in hyphal-inducing growth conditions. We suggest that SIR2 may be involved in Candida albicans quorum-sensing and serum-induced yeast-hyphae transition via the Ras1-cAMP-Efg1 signalling cascade. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Crystale Siew-Ying Lim, Won-Fen Wong, Rozita Rosli, Kee-Peng Ng, Heng-Fong Seow, Pei-Pei Chong Source Type: journals

Wild boars as reservoirs of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli of different phylogenetic groupsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ESBL-producing E. coli isolates have been isolated from eight of seventy seven faecal samples (10.4%) of wild boars in Portugal. The ESBL types identified by PCR and sequencing were blaCTX-M-1 (6 isolates) and blaCTX-M-1 + blaTEM1-b (2 isolates). Further resistance genes detected included tet (A) or tet (B) (in three tetracycline-resistant isolates), aad A (in three streptomycin-resistant isolates), cml A (in one chloramphenicol-resistant isolate), sul 1 and/or sul 2 and/or sul 3 (in all sulfonamide-resistant isolates). The intI 1 gene encoding class 1 integrase was detected in all ESBL-producing E. coli isolates. One isol...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Patrícia Poeta, Hajer Radhouani, Luís Pinto, António Martinho, Vítor Rego, Rogério Rodrigues, Alexandre Gonçalves, Jorge Rodrigues, Vanesa Estepa, Carmen Torres, Gilberto Igrejas Source Type: journals

Elevated curdlan production by a mutant of Agrobacterium sp. ATCC 31749Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A mutant strain of the curdlan-producing bacterium Agrobacterium sp. ATCC 31749, isolated by ethylmethane sulfonate mutagenesis and resistance to ampicillin, was capable of elevated curdlan synthesis. Using 2.5% corn syrup, glucose or maltose as a carbon source, the mutant strain was shown to produce a 1.5-fold, 1.5-fold or 1.5-fold higher level of curdlan, respectively, than its parent strain after 120 h of growth. The mutant strain produced higher curdlan levels after 96 or 120 h of growth on glucose or maltose as a carbon source than it did on corn syrup. Biomass production by the mutant strain grown on the carbon sourc...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Thomas P. West Source Type: journals

Interference of chromium with biological systems in yeasts and fungi: a reviewEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This paper deals with the interactions of chromium (Cr) with biological systems, focusing in particular on yeasts and fungi. These interactions are analysed with primarily regard to biochemical functions, but higher levels of organization are also considered. Thus, the morphological and cytological characteristics of selected microorganisms in response to exposure to chromium ions are evaluated. The different oxidation states of chromium and reactive oxygen species (ROS) generated in redox reactions with chromium ions are presented and characterized. The interactions of the most exposed subcellular structures, including th...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Borut Poljsak, István Pócsi, Peter Raspor, Miklós Pesti Source Type: journals

Genotypic characterization of non starter lactic acid bacteria involved in the ripening of artisanal Bitto PDO cheeseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bitto of Valchiavenna, an artisanal Italian cheese produced without the addition of any starter cultures, has been attributed a protected designation origin (PDO) cheese, but the strain composition of the natural microbial population colonizing this traditional dairy product is still unknown. To obtain preliminary information on the non starter lactic acid bacteria involved in its ripening, a total of 136 NSLAB isolates, randomly selected from MRS and M17 agar plates, were collected from three different cheese samples after 120 days of ripening. The new isolates were identified by combining PCR 16S-23S rDNA spacer analyses...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Francesca Colombo, Francesca Borgo, Maria Grazia Fortina Source Type: journals

Co-production of [gamma] -glutamylcysteine and glutathione by mutant strain Saccharomyces cerevisiae FC-3 and its kinetic analysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Co-production of [gamma] -glutamylcysteine ([gamma] -GC) and glutathione (GSH) by a novel mutant strain Saccharomyces cerevisiae FC-3 and its kinetic analysis were investigated. The strain could produce [gamma] -GC and GSH with high yields (4.22 and 14.3 mg/g-DCW, respectively) in batch submerged cultures. Effects of medium components and cultivation conditions on cell growth and the contents of intracellular [gamma] -GC and GSH were examined. Results show that 2% (w/v) sucrose and 2.5% (w/v) yeast extract are the best carbon and nitrogen sources, respectively, and supplement of three amino acids (glycine, cysteine and glu...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Wen-Chang Chen, Fu-Kuo Huang, Shu-Chuan Cheng, Feng-Yuan Tsai, Chung-Liang Lin Source Type: journals

Efficient inhibition of human cytomegalovirus UL122 gene expression in cell by small interfering RNAsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study is expected to greatly facilitate the use of the RNAi technology for the anti-HCMV studies. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Qun-Jun Duan, Ran Tao, Miao-Feng Hu, Shi-Qiang Shang Source Type: journals

Spontaneous and protein-induced secretion of proteinases from Saccharomyces cerevisiaeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Many fungi are capable of secreting the wide spectrum of hydrolytic enzymes. We characterized an inducible proteinase secretion in yeasts, Saccharomyces cerevisiae. The proteinase secretion by S. cerevisiae was induced in the presence of yeast extract, or of purified proteins, such as bovine serum albumin, casein, or ovalbumin, and some proteolytic activity was present also without protein inducer. We found that properties of proteinases induced under cultivation conditions were different in various aspects (temperature- and pH-dependencies, substrate specificities, sensitivities to proteinase inhibitors). Proteinase activ...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Anita Kurucová, Ester Farka[scaron]ová, [Lcaron]udovít Vare[ccaron]ka, Martin [Scaron]imkovi[ccaron] Source Type: journals

Isolation and identification of a Candida digboiensis strain from an extreme acid mine drainage of the Lignite Mine, GujaratEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An extremely acidic mine drainage (AMD) water sample was collected in 1998 and 2008 from Panandhro lignite mine, Gujarat, India. The yeast isolated from this sample was identified using mini API identification system, as a member of genus Candida. The major cellular fatty acids detected by FAME from the isolate are C16:0 and C18:2 cis 9,12/C18:0[alpha] as 25.23 and 19.5%, respectively. The isolate was identified as Candida digboiensis by 18S rRNA gene sequence analysis and designated as Candida digboiensis SRDyeast1. Phylogenetic analysis using D1/D2 variable domains showed that the closest relative of this strain is Candi...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Mitesh J. Patel, Devayani R. Tipre, Shailesh R. Dave Source Type: journals

Multiresistant Vibrio cholerae non-O1/non-O139 from waters in South India: resistance patterns and virulence-associated gene profilesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study demonstrates the presence of a wide array of critical virulence factors in diverse strains of Vibrio cholerae non-O1/non-O139. Hence, this serogroup can no longer be ignored as an environmental reservoir of virulence genes. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Sankar Jagadeeshan, Praveen Kumar, Wilson Peter Abraham, Sabu Thomas Source Type: journals

Characterization of new biosurfactant produced by Trichosporon montevideense CLOA 72 isolated from dairy industry effluentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The yeast strain CLOA 72 isolated from the effluent of a dairy industry in Brazil and identified as Trichosporon montevideense, was able to grow and produce a glycolipid biosurfactant when cultured on a mineral medium (MM) with sunflower oil as the carbon source. Biosurfactant production was partially growth-associated and maximal emulsification activity was observed at 144 h of cultivation (78.92%). The biosurfactant purified by precipitation with ethanol showed 78.66% emulsifying activity when used in concentrations above 4.5 mg/ml and was able to reduce the surface tension of water to values below 44.9 mN/m. The critica...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Andrea S. Monteiro, Joana O. P. A. Coutinho, Ary C. Júnior, Carlos A. Rosa, Ezequias P. Siqueira, Vera L. Santos Source Type: journals

Detection and characterization of class 1 integrons in Aeromonas spp. isolated from human diarrheic stool in MexicoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We determined the presence of class 1 integrons related to the acquisition of resistance to antimicrobials in Aeromonas spp. isolated from individuals with diarrhea. Species were identified as A. caviae, A. hydrophila, A. veronii and A. media using PCR-RFLP of the 16S rDNA. Selected isolates were further characterized by ERIC-PCR. Resistance to chloramphenicol, aztreonam, tetracycline, trimethoprim/sulfamethoxazole, nalidixic acid and streptomycin, among others, was determined using the Kirby-Bauer method. Integrons were detected by PCR amplification of the 5[prime] conserved, variable, and 3[prime] conserved regions. Sequ...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Abigail Pérez-Valdespino, Elizabeth Fernández-Rendón, Everardo Curiel-Quesada Source Type: journals

Detection of antibiotic resistant E. coli and Enterococcus spp. in stool of healthy growing children in PortugalEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
From stool specimens of 118 healthy children's (1-14 years) in Portugal 92 E. coli and 101 Enterococcu s spp. strains have been isolated. Almost half (40.2%) of the E. coli isolates were resistant to ampicillin, 25.0% were resistant to tetracycline and 26.1% were resistant to streptomycin. Resistance genes detected by specific PCR included blaTEM and/or blaSHV and/or blaCTX-M (33 of 37 ampicillin and/or cefotaxime resistant isolates), tet (A) and/or tet (B) (16 of 23 tetracycline-resistant isolates), aad A (19 of 24 streptomycin-resistant isolates), cml A (in the two chloramphenicol-resistant isolates), aac (3)-II with/wit...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Ângela Barreto, Bruno Guimarães, Hajer Radhouani, Carlos Araújo, Alexandre Gonçalves, Eurico Gaspar, Jorge Rodrigues, Gilberto Igrejas, Patrícia Poeta Source Type: journals

Rapid and sensitive detection of Mollicutes in cell culture by polymerase chain reactionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Infections with Mollicutes species (such as Mycoplasma, Acholeplasma, and Ureaplasma) can induce a variety of problems in living organisms and laboratory cell cultures. Therefore, it is necessary to establish a routine diagnostic protocol for Mycoplasma infection in order to ensure reliable research results, as well as the safety of commercial biological products. For that purpose a novel PCR-based procedure using specific designed primers complementary to 16S rRNA genome region of mollicute species was evaluated. PCR was optimized and sensitivity and specificity was evaluated by defined cell count concentrations (2-31250 ...
Source: Journal of Basic Microbiology - October 5, 2009 Category: Microbiology Authors: Mohammad Hassan Shahhosseiny, Zahra Hosseiny, Hamid Reza Khoramkhorshid, Shahram Azari, Mohammad Ali Shokrgozar Source Type: journals

Screening of Penicillium species for occurrence of lectins and their characterizationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Out of 15 Penicillium species screened for lectin activities, P. griseofulvum and P. thomii were found to possess mycelial lectin activity. None of the species displayed extracellular or cell surface-bound lectin activity. Both species agglutinated rabbit erythrocytes. P. griseofulvum lectin showed specificity to human type O erythrocytes. While P. thomii lectin specifically agglutinated human type A erythrocytes. Highest lectin activities from P. thomii and P. griseofulvum were expressed after 8 and 7 days of growth, respectively. Lectins from both the species displayed a high binding affinity to chondroitin-6-sulphate, m...
Source: Journal of Basic Microbiology - September 30, 2009 Category: Microbiology Authors: Ram Sarup Singh, Sonia Sharma, Gagandeep Kaur, Ranjeeta Bhari Tags: Research Papers Source Type: journals

Diversity of endophytic yeasts from sweet orange and their localization by scanning electron microscopyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Endophytes are microorganisms that colonize plant tissues internally without causing harm to the host. Despite the increasing number of studies on sweet orange pathogens and endophytes, yeast has not been described as a sweet orange endophyte. In the present study, endophytic yeasts were isolated from sweet orange plants and identified by sequencing of internal transcribed spacer (ITS) rRNA. Plants sampled from four different sites in the state of São Paulo, Brazil exhibited different levels of CVC (citrus variegated chlorosis) development. Three citrus endophytic yeasts (CEYs), chosen as representative examples of the is...
Source: Journal of Basic Microbiology - September 30, 2009 Category: Microbiology Authors: Cláudia Santos Gai, Paulo Teixeira Lacava, Walter Maccheroni Jr., Chirlei Glienke, Welington Luiz Araújo, Thomas Albert Miller, João Lúcio Azevedo Tags: Research Papers Source Type: journals

Contents: J. Basic Microbiol. 5/2009Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
No Abstract (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - September 30, 2009 Category: Microbiology Tags: Contents Source Type: journals

Cover Picture: J. Basic Microbiol. 5/2009Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Scanning electron micrographs of endophytic yeast cells of the species Cryptococcus flavescens colonizing citrus plants after inoculation. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - September 30, 2009 Category: Microbiology Tags: Cover Picture Source Type: journals

Classification of new BVDV isolates from Philippine water buffalo using the viral E2 regionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Characterization of bovine viral diarrhea virus (BVDV) isolates has been focused of several studies this last decade. Until now lots of new strains are being unfolded maybe due to the viral fast mutation ability. As we focused our research on water buffalo immunology, we were able to identify a probable new BVDV isolates. RNA was extracted from water buffalo blood in the Philippines. The extracted RNA was reverse-transcribed and synthesized cDNA. Oligonucleotide primers from the viral E2 region were used to amplify the target viral gene and later purified, cloned and sequenced. The E2 region with 420 bp nucleotides long wa...
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: Claro N. Mingala, Satoru Konnai, Motoshi Tajima, Misao Onuma, Kazuhiko Ohashi Source Type: journals

Purification and properties of an endoglucanase of Aspergillus terreus DSM 826Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Endoglucanase (EG) from A. terreus DSM 826 grown on sugar cane bagasse as a carbon source was purified using acetone fractionation, then a Sepharose-4B chromatographic column, with purification of about 27-fold and 10.5% recovery. The optimum temperature and pH for activity of the purified EG were found to be 50 °C and pH 4.8, respectively. The purified enzyme can stand heating up to 50 °C for 1 h without apparent loss of activity. However, the enzyme, incubated at 80 °C for 5 min, showed about 56% loss of activity. Optimum EG activity was recorded with a citrate buffer system (pH 4.8; 0.05 M). Co2+ (2.5 × 10-2 M) and ...
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: Ali M. Elshafei, Mohamed M. Hassan, Bakry M. Haroun, Osama M. Abdel-Fatah, Housam M. Atta, Abdelmageed M. Othman Source Type: journals

Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An extracellular lipase from Pseudomonas aeruginosa ATCC 27853 has been purified and its enzymatic characteristics were determined. According to SDS-PAGE and gel filtration molecular mass estimated to be 30 kDa, what classified the lipase in group I.1. Although 14 lipases from P. aeruginosa with similar molecular mass are referred to date, their basic enzymatic properties have not been reported yet. To address the gap we found: the optimal temperature and pH in water solution being 50 °C and 9.3, respectively; the lipase was inhibited with Hg2+ ions and sodium dodecylsulphate (SDS), while non-ionic detergent Triton X-100 ...
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: Lidija T. Izrael-Zivkovic, Gordana [Dstrok]. Gojgic-Cvijovic, Kristina R. Gopcevic, Miroslav M. Vrvic, Ivanka M. Karadzic Source Type: journals

Pioneer colonizer microorganisms in biofilm formation on galvanized steel in a simulated recirculating cooling-water systemEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined biofilm formation and first attachment maintance of biofilms of Pseudomonas spp., Pseudomonas aeruginosa, Aeromonas spp, sulphate reducing bacteria and filamentous fungi. A simulated recirculating cooling-water system was used. Heterotrophic bacteria counts on galvanized steel and glass surfaces rose during the tidy period of 720 hours. In addition, we determined that although Pseudomonas spp., Pseudomonas aeruginosa and Aeromonas spp. were the pioneer colonizers, they surprisingly could not be determined in the biofilms on both types of surface after 456 hours. Sulphate reducing bacteria were observed in biofi...
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: Nihal Do[gbreve]ruöz, Duygu Göksay, Esra Ilhan-Sungur, Ay[scedil][inodot]n Cotuk Source Type: journals

Differential response of antioxidative defense system of Anabaena doliolum under arsenite and arsenate stressEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, the cyanobacterium may survive better in As(V) than As(III) contaminated fields because of its low toxicity and pronounced induction of antioxidative defense system. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: Ashish Kumar Srivastava, Poonam Bhargava, Riti Thapar, Lal Chand Rai Source Type: journals

Reduction of molybdate to molybdenum blue by Enterobacter sp. strain Dr.Y13Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Extensive use of metals in various industrial applications has caused substantial environmental pollution. Molybdenum-reducing bacteria isolated from soils can be used to remove molybdenum from contaminated environments. In this work we have isolated a local bacterium with the capability to reduce soluble molybdate to the insoluble molybdenum blue. We studied several factors that would optimize molybdate reduction. Electron donor sources such as glucose, sucrose, lactose, maltose and fructose (in decreasing efficiency) supported molybdate reduction after 24 h of incubation with optimum glucose concentration for molybdate r...
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: M.Y. Shukor, M.F. Rahman, N.A. Shamaan, M.A Syed Source Type: journals

L-methioninase production by Aspergillus flavipes under solid-state fermentationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Solid-state fermentation was carried out for the production of extra-cellular L-methioninase by Aspergillus flavipes (Bain and Sart.) using nine agro-industrial residues, namely wheat bran, rice bran, wheat flour, coconut seeds, cotton seeds, ground nut cake, lentil hulls, soya beans and chicken feathers. Chicken feathers were selected as solid substrate for L-methioninase production by A. flavipes. The maximum L-methioninase productivity (71.0 U/mg protein) and growth (11 mg protein/ml) of A. flavipes was obtained using alkali pretreated chicken feathers of 50% initial moisture content as substrate supplemented with D-glu...
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: Ashraf S. A. El-Sayed Source Type: journals

The Legionella pneumophila Dps homolog is regulated by iron and involved in multiple stress toleranceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we identified the putative homolog of the iron-storage protein-encoding gene, dpsL, in the intracellular pathogen Legionella pneumophila and demonstrated its expression under iron-limited conditions and its responses to multiple stresses. Quantitative real-time PCR analysis indicated that the expression of dpsL was enhanced under iron limitation regardless of the growth phase. Compared with the wild-type cells, the cells devoid of dpsL were heat and H2O2-sensitive. In contrast to the dps mutants of other bacteria, the growth of the dpsL mutant in an iron-deprived medium was delayed but finally reached the sa...
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: Ming-jia Yu, Jun Ren, Yong-lun Zeng, Shi-ning Zhou, Yong-jun Lu Source Type: journals

Phenotypic and genotypic characterizations of rhizobia isolated from root nodules of peanut (Arachis hypogaea L.) grown in Moroccan soilsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The phenotypic and genotypic characterization of sixty-two rhizobial isolates obtained from nodules of Arachis hypogaea in north-western Morocco was performed. Their physiological and biochemical properties revealed a great deal of diversity among them. Isolates were classified into two major groups based on the numerical analysis of their phenotypic and genotypic characteristics. Isolates in the first group were alkali- and salt-sensitive, slow or extra-slow growers; they did not use disaccharides as carbon source and varied in the use of amino acids. ARDRA analysis of the 16S rDNA region grouped them together with refere...
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: M. Rabie El-Akhal, Ana Rincon, Nourdin El Mourabit, Jose J. Pueyo, Said Barrijal Source Type: journals

Comparative proteomic analysis of passaged Helicobacter pyloriEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to identify the proteins associated with Helicobacter pylori colonization in mice, we used 2-dimensional gel electrophoresis (2-DE) to analyze the membrane- and soluble-cellular proteins extracted from H. pylori strain 26695 and the mouse-passaged homolog 88-3887. We defined 2- and 3-fold changes in protein expression as the threshold values for differential expression in the membrane-protein and whole-cell-protein fractions, respectively. The differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF). A total of 29 proteins, in...
Source: Journal of Basic Microbiology - May 19, 2009 Category: Microbiology Authors: Mao-Jun Zhang, Fei Zhao, Di Xiao, Yi-Xin Gu, Fan-Liang Meng, li-Hua He, Jian-Zhong Zhang Source Type: journals

Phenotypic and genotypic characterizations of rhizobia isolated from root nodules of peanut (Arachis hypogaea L.) grown in Moroccan soilsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The phenotypic and genotypic characterization of sixty-two rhizobial isolates obtained from nodules of Arachis hypogaea in north-western Morocco was performed. Their physiological and biochemical properties revealed a great deal of diversity among them. Isolates were classified into two major groups based on the numerical analysis of their phenotypic and genotypic characteristics. Isolates in the first group were alkali- and salt-sensitive, slow or extra-slow growers; they did not use disaccharides as carbon source and varied in the use of amino acids. ARDRA analysis of the 16S rDNA region grouped them together with refere...
Source: Journal of Basic Microbiology - May 18, 2009 Category: Microbiology Authors: M. Rabie El-Akhal, Ana Rincon, Nourdin El Mourabit, Jose J. Pueyo, Said Barrijal Tags: Research Papers Source Type: journals

A novel way to enhance the oil recovery ratio by Streptococcus sp. BT-003Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Degrading hydrocarbon by Streptococcus sp. BT-003 as a kind of microbe for oil recovery was analyzed in this paper. The Streptococcus sp. BT-003 showed that it could use crude oil as the sole source of carbon and produce organic acid, bio-gas and polysaccharide which were propitious to emulsify and reduce the viscosity of crude oil. After cultivating 8-14 h, the viscosity of crude oil reduced from 8000-15000 mPa · s to 50-250 mPa · s. The content of organic acid increased 8-15 times, and carbon dioxide and polysaccharide reached 55 ml/l and 8 g/l respectively. Paraffin and resin reduced by 60-95%, and light components in...
Source: Journal of Basic Microbiology - March 29, 2009 Category: Microbiology Authors: Tao Xu, Chang Chen, Chunqiao Liu, Shurong Zhang, Ying Wu, Peng Zhang Source Type: journals

Direct analysis of sulfate reducing bacterial communities in gas hydrate-impacted marine sediments by PCR-DGGEEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study exemplifies the numerical and functional importance of sulfate reducing bacteria in deep-sea sedimentary environments, and incremental methodological advancements, as described herein, will continue to streamline the analysis of sulfate reducer communities in situ. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Christopher E. Bagwell, Michael Formolo, Qi Ye, Chris M. Yeager, Timothy W. Lyons, Chuanlun L. Zhang Source Type: journals

Genetic characterization of vancomycin-resistant enterococci isolates from wild rabbitsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The presence of van A-containing E. faecium isolates was demonstrated in three of 77 faecal samples (3.9%) of wild rabbits recovered in Portugal. Enterococcal strains with intrinsic vancomycin resistance (van C-1 or van C-2/3 gene) were found in five (6.5%) and three (3.9%) faecal samples, respectively. The mechanisms of resistance for other antibiotics were studied in these vancomycin-resistant isolates. All van A strains showed resistance for tetracycline [with the presence of tet (L) gene, associated or not with tet (M) gene] and for erythromycin [with the presence of the erm (B) gene]. Two isolates were resistant to ci...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Nicholas Figueiredo, Hajer Radhouani, Alexandre Gonçalves, Jorge Rodrigues, Carlos Carvalho, Gilberto Igrejas, Patrícia Poeta Source Type: journals

Cadmium biosorption by Streptomyces sp. F4 isolated from former uranium mineEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study could present the potential capacity of Streptomyces sp. F4 for Cd2+ bioremediation. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) (Source: Journal of Basic Microbiology)
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Manuel Siñeriz Louis, Erika Kothe, Carlos Mauricio Abate Source Type: journals

Biodegradation of hazardous triphenylmethane dye methyl violet by Rhizobium radiobacter (MTCC 8161)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rhizobium radiobacter MTCC 8161 completely decolorized methyl violet (10 mg l-1) within 8 h both at static and shaking conditions. The decolorization time increased with increasing dye concentration. The effect of different carbon and nitrogen sources on the decolorization of methyl violet was studied. The maximum decolorization was observed in the presence of sucrose (1%) and urea (1%). UV-Visible, HPLC and FTIR analysis of extracted products confirmed biodegradation of methyl violet. The significant increase in the activities of lignin peroxidase and aminopyrine N-demethylase in the cells obtained after decolorization in...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Ganesh Parshetti, Ganesh Saratale, Amar Telke, Sanjay Govindwar Source Type: journals

Cloning, sequencing and expression of full-length Campylobacter invasion antigen B gene operon from Campylobacter lariEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A novel PCR primer pair for amplification of full-length cia B gene from thermophilic campylobacters, generated an amplicon of approximately 2.2 kilo base pairs (kbp) with all 18 isolates (n = 7 for urease-negative (UN) C. lari; n = 9 urease-positive thermophilic Campylobacter (UPTC); n = 1 C. jejuni; n = 1 C. coli). The putative open reading frame (ORF) of the cia B from C. lari isolates consisted of 1,833 bp similarly, but differing from those of C. jejuni and C. coli isolates. The putative promoter structures consisting of a semi-conserved T -rich sequence and a consensus sequence at the -10 region were identified upstr...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Junnosuke Onozato, Atsuko Kumagai, Tsuyoshi Sekizuka, Akihiro Tazumi, John E. Moore, B. Cherie Millar, Motoo Matsuda Source Type: journals

Responses of Candida fukuyamaensis RCL-3 and Rhodotorula mucilaginosa RCL-11 to copper stressEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The effect of high Cu(II) concentrations on superoxide dismutase (SOD) and catalase (CAT) activity in Candida fukuyamaensis RCL-3 and Rhodotorula mucilaginosa RCL-11, previously isolated from a copper filter at a mine plant in Argentina, was studied. Addition of 0.1, 0.2 and 0.5 mM Cu(II) to the culture medium increased total SOD and CAT activity in both strains. Native polyacrylamide gel electrophoresis revealed two bands with SOD activity for C. fukuyamaensis RCL-3 and only one for R. mucilaginosa RCL-11; the three bands corresponded to MnSOD.Intracellular accumulation of copper and morphological changes was observed usi...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Liliana B. Villegas, María J. Amoroso, Lucía I. C. de Figueroa Source Type: journals

Evidence of methanesulfonate utilizers in the Sargasso Sea metagenomeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Methanesulfonate (MSA) is one of the products of the photo-oxidation of dimethylsulfide in the atmosphere. The genes responsible for the import of MSA into the cell (msm EFGH) and for its oxidation to formaldehyde (msm ABCD) have been previously sequenced from the soil bacterium Methylosulfonomonas methylovora str. M2 while genes for an MSA monooxygenase have been sequenced from marine bacterium Marinosulfonomonas methylotropha str. TR3. We performed a sequence-based screening of the Sargasso Sea metagenome for homologues of the MSA monooxygenase (MSAMO) and MSA import genes. Our search retrieved one scaffold bearing genes...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Elsa Leitão, Pedro Moradas-Ferreira, Paolo De Marco Source Type: journals

Demonstration of the absence of intervening sequences within 23S rRNA genes from Campylobacter lariEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cloning, sequencing and characterization of nearly full-length 23S rRNA genes in 12 urease-positive thermophilic Campylobacter (UPTC) isolates were carried out using two novel PCR primer pairs. Nucleotide sequences of the 23S rRNA genes from the 12 isolates were first shown not to carry any intervening sequences (IVSs) in both the 25 and 45 helix regions. Then, two PCR primer sets were designed in silico for amplification of the helix 25 and 45 regions within 23S rRNA gene sequences from Campylobacter lari. No IVSs were identified within the 23S rRNA genes among a total of 53 isolates of C. lari, following PCR amplificatio...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Akihiro Tazumi, Yuki Kakinuma, John E. Moore, Cherie B. Millar, Ikue Taneike, Motoo Matsuda Source Type: journals

Biodegradation of diisodecyl phthalate (DIDP) by Bacillus sp. SB-007Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, diisodecyl phthalate (DIDP) was efficiently degraded by Bacillus sp. SB-007. The optimal conditions for DIDP (100 mg l-1) degradation by Bacillus sp. SB-007 in a mineral salts medium were found to be pH 7.0 at 30 °C, stirring at 200 rpm. The specific rate of DIDP degradation was found to be concentration dependent with a maximum of 4.87 mg DIDP l-1 h-1. DIDP was transformed rapidly by Bacillus sp. SB-007 with the formation of monoisodecyl phthalate and phthalic acid, which subsequently degraded further. These results highlight the potential of this bacterium for removing DIDP contaminated waste in the envir...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Jae-Min Park, Mina Kim, Jihee Yoon, Fumihisa Kobayashi, Yasunobu Iwasaka, Chun-Sang Hong, Jiho Min, Yang-Hoon Kim Source Type: journals

A calcium binding protein from cell wall of Neurospora crassaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Isolated cell wall preparations of N. crassa bind significant levels of Ca, Mg and other divalent cations. Enzymatic treatment of the cell wall with [beta]-(1,3)-glucanase, but not with chitinase, resulted in solubilization of only the calcium-binding protein fraction. A calcium-binding protein (CaBP) was purified by metal-chelate affinity chromatography and reversed phase HPLC. CaBP has an Mr of around 6 kDa on SDS-PAGE and mass spectrometry showed that it has a molecular mass of 5744 Da. One mole of CaBP binds 2 moles of calcium and is partially inhibited (15-50%) by other divalent cations (Mg, Ni and Cu). Quenching of t...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Thommandru Naga Sowjanya, Pamarthi Maruthi Mohan Source Type: journals

Diversity and phylogeny of culturable spore-forming Bacilli isolated from marine sedimentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Members of the genus Bacillus and related genera are ubiquitous in nature. However, Bacillus species isolated from marine sediments have attracted less interest respect to their terrestrial relatives. Here, we report the phylogenetic diversity of a collection of 96 Bacilli, isolated from 17 distinct stations of 5 oceanographic campaigns. The diversity was analysed by phenotypic and molecular approaches based on the amplified rDNA restriction analysis (ARDRA), amplification of the internal transcribed spacers (ITS-PCR) and on 16S rRNA sequencing. Intra-specific polymorphism was efficiently detected by biochemical analysis a...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Besma Ettoumi, Noura Raddadi, Sara Borin, Daniele Daffonchio, Abdellatif Boudabous, Ameur Cherif Source Type: journals

Destruxin production of Metarhizium anisopliae under carbon and nitrogen exhaustionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Destruxins (dtx) A, B, and E, showing a variety of biological activities, are the main toxic secondary metabolites of the entomopathogenous ascomycete Metarhizium anisopliae Bipesco 5, a widely used biocontrol production strain. Dynamics of dtx biosynthesis were monitored during liquid fermentation in a chemically defined medium. During shake flask cultivation with excess carbon, nitrogen and phosphate, approximately 50, 20, and 100 mg l-1 dtx A, B, and E were produced after 12 d. Destruxins were produced during exponential growth phase and in the stationary phase. Carbon exhaustion in the culture broth was demonstrated to...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Hui Wang, Stefan Hutwimmer, Hermann Strasser, Wolfgang Burgstaller Source Type: journals

Laccase production by Pycnoporus sanguineus under different culture conditionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pycnoporus sanguineus is a white-rot fungus that produces ligninolytic enzymes such as laccases. These enzymes can endure temperatures as high as 60 °C and are useful for pulp bleaching, dye decolorization and phenolic degradation.Laccase production by fungi depends not only on the carbon and nitrogen sources but also on the nitrogen concentration of the culture medium. In this work, we examined the effect of four carbon sources (maltose, glucose, fructose and sucrose) and four nitrogen sources (ammonium tartrate, sodium nitrate, asparagine and yeast extract) on the activity of laccase from Pycnoporus sanguineus. All carb...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: María Eugenia Eugenio, José María Carbajo, Juan Antonio Martín, Aldo Enrique González, Juan Carlos Villar Source Type: journals

Molecular diversity of a native mesorhizobial population of nodulating chickpea (Cicer arietinum L.) in Indian soilsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chickpea plants with nodules were collected from 32 different farmers' fields of eight districts of Haryana state. In total, 137 mesorhizobial isolations were made from these nodules and authenticated. Finally, 50 mesorhizobia were selected based on nodulation test, growth characteristics, and site of sampling. The molecular diversity of the mesorhizobial population was assessed by PCR-amplified ERIC profiles as well as RFLP of 16S rDNA. Considerable molecular diversity in Haryana soils was observed. Chickpea rhizobia were grouped into six different clusters at the 70% similarity level by both methodologies, but clustering...
Source: Journal of Basic Microbiology - March 25, 2009 Category: Microbiology Authors: Rachna Nandwani, S. S. Dudeja Source Type: journals

Isolation and preliminary probiotic selection of lactobacilli from koumiss in Inner MongoliaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
From 16 samples of traditional fermented koumiss collected in Inner Mongolia Autonomous Region of China, forty-eight lactobacilli strains were isolated and phenotypically characterized by their abilities to ferment different carbohydrates and by additional biochemical tests. The dominant lactobacilli species were identified as L. casei (17 strains), L. helveticus (10 strains) and L. plantarum (8 strains), with a lower frequency of isolation for L. coryniformis subsp. coryniformis (5 strains), L. paracasei (3 strains), L. kefiranofaciens (2 strains), L. curvatus (1 strain), L. fermentum (1 strain) and W. kandleri (1 strain)...
Source: Journal of Basic Microbiology - February 13, 2009 Category: Microbiology Authors: Rina Wu, Liping Wang, Jicheng Wang, Haiping Li, Bilige Menghe, Junrui Wu, Mingruo Guo, Heping Zhang Source Type: journals

Effect of nickel on the mineralization of hydrocarbons by indigenous microbiota in Kuwait soilsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Assessment of nickel contents in soil samples in Kuwait indicated only a minor difference in concentration in hydrocarbon-contaminated (86 mg kg-1 soil) and non-contaminated soils (84 mg kg-1 soil). The potential inhibitory effects of nickel on the number of hydrocarbon degraders, and hydrocarbon utilization were investigated over a wide range of nickel concentrations to span concentrations observed in the soil. Nickel addition, as nickel sulphate, to soil samples reduced the number of hydrocarbon degraders in all samples by a wide range (15-96%) depending on concentration and the hydrocarbon substrate utilized. Similarly,...
Source: Journal of Basic Microbiology - February 13, 2009 Category: Microbiology Authors: Esmaeil S. AL-Saleh, Christian Obuekwe Source Type: journals

Quantitative volatile metabolite profiling of common indoor fungi: relevancy for indoor air analysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Microorganisms such as bacteria and molds produce an enormous variety of volatile metabolites. To determine whether typical microbial volatile metabolites can be used as indicator compounds for the detection of hidden mold in indoor environments, we examined 14 typical indoor fungal strains for their growth rates and their capability to produce volatile organic compounds (VOC) on standard clinical media and on agar medium made from building materials. Air samples from Headspace Chambers (HSC) were adsorbed daily on Tenax TA tubes and analyzed by thermal desorption gas chromatography and mass spectrometry. In parallel, meta...
Source: Journal of Basic Microbiology - February 13, 2009 Category: Microbiology Authors: Sven Schuchardt, Hermann Kruse Source Type: journals

Genotypic and phenotypic characterization of Lactobacillus plantarum strains isolated from Thai fermented fruits and vegetablesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ten Lactobacillus strains originally isolated from Thai fruits and vegetables fermentation were characterized by various phenotypic and genotypic methods. The phenotypic analysis using the method of carbohydrate fermentation patterns (API50CHL) revealed that the isolates belonged to the L. plantarum species. This was further confirmed by 16S rRNA gene sequencing. Multilocus sequence typing (MLST) revealed a strongly clonal population structure and a low genotypic diversity in this collection. However, the analyzed L. plantarum population demonstrated a higher level of diversification after API50CHL that reflects the role o...
Source: Journal of Basic Microbiology - February 13, 2009 Category: Microbiology Authors: Winee Tanganurat, Benoit Quinquis, Vichien Leelawatcharamas, Alexander Bolotin Source Type: journals

The role of extracellular polymers on Staphylococcus epidermidis biofilm biomass and metabolic activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the biofilm matrix of four S. epidermidis clinical isolates was extracted and the polysaccharides and proteins content was quantified. The results were correlated with the total biofilm biomass (determined by crystal violet assay) and cellular metabolic activity (evaluated with XTT reduction assay). According to the results, the exopolymers studied play an important role not only on structure and biofilm biomass but also on cellular activity. Thus, the strain forming biofilms with the highest level of polysaccharides (S. epidermidis 1457) also formed thicker biofilms but with the lowest metabolic activity. T...
Source: Journal of Basic Microbiology - February 13, 2009 Category: Microbiology Authors: Cláudia Sousa, Pilar Teixeira, Rosário Oliveira Source Type: journals