Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
