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Endothelin-1, big endothelin-1, and nitric oxide in patients with chronic renal disease and hypertensionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The complex pathogenesis of chronic renal disease (CRD) depends on endothelin (ET) axis (ETs and ET receptors) and nitric oxide (NO) because of their vasoactive effects and their role in general modulation of vascular homeostasis. Various renal cells synthesize ETs and NO that play a significant role in renal hemodynamics as well as in water and salt excretion via urine. ET-1 is a strong vasoconstrictor. Besides its vasoactive effects, ET-1 modulates mitosis and apoptosis in a cell type-dependent manner, and may play an important role in CRD pathogenesis. The aims of this study were to emphasize the role and interactions o...
Source: Journal of Clinical Laboratory Analysis - November 20, 2009 Category: Laboratory Medicine Authors: Ivanka Mikuli[cacute], József Petrik, Kre[scaron]imir Gale[scaron]i[cacute], [Zcaron]eljko Romi[cacute], Ivana [Ccaron]epelak, Monika Zeljko-Tomi[cacute] Tags: Original Articles Source Type: journals

IgM, IgG, and IgA anti-DNA topoisomerase I antibodies in systemic sclerosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This report confirms that different immunoglobulin classes with anti-topo I activity may occur in SSc. IgG was the predominant serological feature with both, the recombinant and native antigens. The study also demonstrates the association between high levels of these autoantibodies and some clinical manifestations of SSc. J. Clin. Lab. Anal. 23:408-416, 2009. © 2009 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Erasmo Martínez-Cordero, Alejandro Padilla Trejo, Diana E. Aguilar León Tags: Original Articles Source Type: journals

Prevalence characterization of extended-spectrum [beta]-lactamases among Escherichia coli isolates collected in ZhengzhouEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We describe a complex ESBL epidemiology. The study revealed a high rate of ESBL-producing E. coli isolates. TEM and CTX-M enzymes dominated in ESBL-positive E. coli isolates in Zhengzhou, China. J. Clin. Lab. Anal. 23:404-407, 2009. © 2009 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Zhijian Zhang, Xiaobing Guo, Qinxian Zhang Tags: Original Articles Source Type: journals

New method for calcium on the ADVIA analyzer is free from interference of gadolinium-type contrast agentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recently, Siemens Diagnostics released a new calcium assay (CA_2) based on complex formation of calcium with Arsenazo III dye for use on the three automated, random access ADVIA Chemistry analyzers (1650, 2400, and 1200). We evaluated this method for analytical performance as well as potential interference from gadolinium-containing magnetic contrast agents. With Siemens Chemistry serum and urine controls, 2-levels each, the imprecision for the new method was (n=40 each): within-run and total CV of (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Pradip Datta, Amitava Dasgupta Tags: Original Articles Source Type: journals

Rapid liquid chromatography/tandem mass spectrometer (LCMS) method for clozapine and its metabolite N-desmethyl Clozapine (Norclozapine) in human serumEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study describes a simple liquid chromatography/tandem mass spectrometer (LCMS) method in human serum. The ion transitions monitored were m/z 327, 270, 296 for Clozapine, m/z 313, 192, 227 for Norclozapine and m/z 328, 271 for Loxapine. The assay is linear (25-1000 ng/ml) and showed a good correlation (r=0.98) within the analytical range of 79-1210 ng/ml in human serum. This assay is highly specific and sensitive for the simultaneous measurements of Clozapine and Norclozapine. The simplification of this assay makes it ideal for high throughput analyses of the patient samples in a routine clinical laboratory staffed wit...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: L.V. Rao, M.L. Snyder, G.M. Vallaro Tags: Original Articles Source Type: journals

CXCL12 rs1801157 polymorphism in patients with breast cancer, hodgkin's lymphoma, and non-hodgkin's lymphomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chemokines and their receptors regulate the trafficking of immune cells during their development, inflammation, and tissue repair. The single-nucleotide polymorphism (SNP) rs1801157 (previously known as CXCL12-A/ stromal cell-derived factor-1 (SDF1)-3[prime]A) in CXCL12/SDF1 gene was assessed in breast cancer, Hodgkin's lymphoma (HL), and non-Hodgkin's lymphoma (NHL), since the chemokine CXCL12, previously known as SDF1, and its receptor CXCR4 regulate leukocyte trafficking and many essential biological processes, including tumor growth, angiogenesis, and metastasis of different types of tumors. Genotyping was performed by...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Karen Brajão de Oliveira, Julie Massayo Maeda Oda, Julio Cesar Voltarelli, Thiago Franco Nasser, Mario Augusto Ono, Thiago Cezar Fujita, Tiemi Matsuo, Maria Angelica Ehara Watanabe Tags: Original Articles Source Type: journals

Gene diagnosis for nine Chinese patients with DMD/BMD by multiplex ligation-dependent probe amplification and prenatal diagnosis for one of themEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aims to perform gene diagnosis for nine patients with Duchenne/Becker muscular dystrophy (DMD/BMD) and their parents with multiplex ligation-dependent probe amplification (MLPA), and to carry out prenatal gene diagnosis for one of them. Genomic DNA of the peripheral blood and fetal amniotic fluid cell was extracted from the pedigrees' members with DMD/BMD. Gene diagnosis was performed for theses pedigrees' members using a SALSA KIT. Short tandem repeats (STR) genotyping and X-linkage analysis were performed for the pedigree members of the fetus, which was used in the prenatal diagnosis. MLPA analysis results sho...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Yupeng Wu, Gengxin Yin, Keqin Fu, De Wu, Qian Zhai, Huarong Du, Zhongjun Huang, Yuhua Niu Tags: Original Articles Source Type: journals

Increased soluble serum markers caspase-cleaved cytokeratin-18, histones, and ST2 indicate apoptotic turnover and chronic immune response in COPDEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we hypothesized that systemic markers of apoptosis are altered in patients with mild-to-severe COPD.Materials and Methods: A total number of 64 patients and controls were enrolled in the study. Lung function parameters of all groups (nonsmoker, healthy smoker, COPD GOLD I&II, COPD GOLD III&IV) were evaluated at the time of inclusion. Enzyme-linked immunosorbent assays were used to quantify protein levels in serum samples.Results: Serum contents of apoptotic end-products caspase-cleaved cytokeratin-18 and histone-associated-DNA-fragments were increased in patients with COPD, whereas anti-inflammatory soluble ...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Stefan Hacker, Christopher Lambers, Andreas Pollreisz, Konrad Hoetzenecker, Michael Lichtenauer, Andreas Mangold, Tina Niederpold, Andreas Hacker, György Lang, Martin Dworschak, Thomas Vukovich, Christopher Gerner, Walter Klepetko, Hendrik Jan Ankersmit Tags: Original Articles Source Type: journals

AML1 amplification and 17q25 deletion in a case of childhood acute lymphoblastic leukemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a case of childhood acute lymphoblastic leukemia (ALL) with both acute myeloid leukemia 1 (AML1) amplification and 17q25 deletion. AML1 gene is located on 21q22 and encodes a transcription factor. AML1 amplification is a common finding in childhood ALL, and itis observed as an increase in gene copy number by the FISH analysis. The mechanism of AML1 amplification is not associated with AML1 gene mutations. The 17q25 is a gene-rich chromosomal location and distinct abnormalities of this region have been observed in previous cases of different kinds of leukemia. Deletion of the 17q25 region has been reported in two ...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Tuna Gulten, Tahsin Yakut, Mutlu Karkucak, Birol Baytan, Adalet Meral Gune[scedil] Tags: Original Articles Source Type: journals

A novel genetic variant of BMP2K contributes to high myopiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Loss of eye growth regulation may cause myopia, because modulation of optic globe size is essential for the generation of normal optic power. Evidence has implied variations of BMP2 gene expression mediate ocular development and retinal tissue remodeling. Given BMP2 as a potential regulator involved in myopia development, we investigate whether gene BMP2-inducible kinase (BMP2K, BIKe), whose expression is up-regulated during BMP2-induced osteoblast differentiation, contributes to susceptibility of high myopia. Participants grouped into high myopia had a spherical equivalent greater than -6.00 D, compared with a control gro...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Hsin-Ping Liu, Ying-Ju Lin, Wei-Yong Lin, Lei Wan, Jim Jinn-Chyuan Sheu, Hui-Ju Lin, Yuhsin Tsai, Chang-Hai Tsai, Fuu-Jen Tsai Tags: Original Articles Source Type: journals

Human epididymis protein 4 is a biomarker for transitional cell carcinoma in the urinary systemEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Objective: To investigate human epididymis protein 4 (HE4) levels in transitional cell carcinoma (TCC) of the urinary system and its relationship with clinicopathological features.Methods: 102 patients with TCC, 60 with benign urinary diseases, and 60 healthy controls were included in this study. The HE4 levels were used to analyze different clinicopathologic characteristics and changes between pre- and postsurgical operation.Results: The HE4 level was significantly increased in patients with TCC compared to patients with benign urinary diseases patients (P (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Zhu Xi, Ma LinLin, Tian Ye Tags: Original Articles Source Type: journals

Bone marrow aspirate and biopsy: a pathologist's perspective. II. interpretation of the bone marrow aspirate and biopsyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bone marrow examination has become increasingly important for the diagnosis and treatment of hematologic and other illnesses. Morphologic evaluation of the bone marrow aspirate and biopsy has recently been supplemented by increasingly sophisticated ancillary assays, including immunocytochemistry, cytogenetic analysis, flow cytometry, and molecular assays. With our rapidly expanding knowledge of the clinical and biologic diversity of leukemia and other hematologic neoplasms, and an increasing variety of therapeutic options, the bone marrow examination has became more critical for therapeutic monitoring and planning optimal ...
Source: Journal of Clinical Laboratory Analysis - September 23, 2009 Category: Laboratory Medicine Authors: Roger S. Riley, David Williams, Micaela Ross, Shawn Zhao, Alden Chesney, Bradly D. Clark, Jonathan M. Ben-Ezra Tags: Original Articles Source Type: journals

Comparison of the influence of thyroglobulin antibodies on serum thyroglobulin values from two different immunoassays in post surgical differentiated thyroid carcinoma patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Measurement of serum thyroglobulin (Tg) is a highly specific test in the management of patients with differentiated thyroid cancer (DTC) after surgical treatment. The aim of our study was to evaluate and compare Tg levels in these patients found by radioimmunoassay (RIA) and immunoradiometric assay (IRMA) and to assess the influence of Tg antibodies (TgAbs) on the values obtained for Tg concentration. Both Tg and TgAb were determined postoperatively in the serum of 71 DTC patients using RIA Tg-PEG (INEP) and Tg IRMA (CIS) for Tg, together with TgAb (CIS) for circulating endogenous anti-TgAbs. The obtained concentrations we...
Source: Journal of Clinical Laboratory Analysis - September 21, 2009 Category: Laboratory Medicine Authors: Marijana Stanojevic, Svetlana Savin, Dubravka Cvejic, Aleksandar Djukic, Marija Jeremic, Snezana Zivancevi[cacute] Simonovic Tags: Original Articles Source Type: journals

Evaluation of DPC immulite 2000® Toxoplasma quantitative IgG/IgM kits for automated toxoplasmosis serology with immulite 2000®Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we evaluated a new immunoassay for the automated detection of anti-toxoplasma IgG and IgM with Immulite 2000® (DPC-Siemens, La Garenne-Colombes, France). We tested 280 sera from 112 patients with past infection (PI), 40 PI with residual IgM, 75 seronegatives, 16 infants (31 sera) monitored for neonatal screening of congenital toxoplasmosis, and 13 patients with recent seroconversion (SC) (22 sequential sera). Detection sensitivity and specificity for IgG were 99 and 100%, respectively. IgG titers obtained with Immulite 2000® were higher than with Vidas® (BioMérieux, Marcy I'Etoile, France) and Access® (...
Source: Journal of Clinical Laboratory Analysis - September 21, 2009 Category: Laboratory Medicine Authors: Florence Robert-Gangneux, Chantal Bourhis, Sylviane Chevrier, Jean-Pierre Gangneux Tags: Original Articles Source Type: journals

The utility of serum human epididymis protein 4 (HE4) in patients with a pelvic massEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: HE4 seems to be a promising ovarian cancer marker, and its measurement might improve the diagnostic approach to patients with pelvic masses. J. Clin. Lab. Anal. 23:331-335, 2009. © 2009 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - September 21, 2009 Category: Laboratory Medicine Authors: Martina Montagnana, Giuseppe Lippi, Orazio Ruzzenente, Valentina Bresciani, Elisa Danese, Silvia Scevarolli, Gian Luca Salvagno, Silvia Giudici, Massimo Franchi, Gian Cesare Guidi Tags: Original Articles Source Type: journals

Correlation between Etest®, disk diffusion, and microdilution methods for antifungal susceptibility testing of Candida species from infection and colonizationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The correlation between the microdilution (MD), Etest® (ET), and disk diffusion (DD) methods was determined for amphotericin B, itraconazole and fluconazole. The minimal inhibitory concentration (MIC) of those antifungal agents was established for a total of 70 Candida spp. isolates from colonization and infection. The species distribution was: Candida albicans (n=27), C. tropicalis (n=17), C. glabrata (n=16), C. parapsilosis (n=8), and C. lusitaniae (n=2). Non-Candida albicans Candida species showed higher MICs for the three antifungal agents when compared with C. albicans isolates. The overall concordance (based on the ...
Source: Journal of Clinical Laboratory Analysis - September 21, 2009 Category: Laboratory Medicine Authors: Melyssa Negri, Mariana Henriques, Terezinha I.E. Svidzinski, Claudete Rodrigues Paula, Rosário Oliveira Tags: Original Articles Source Type: journals

Oxidative status in iron-deficiency anemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oxidative stress is an imbalance between free radicals and antioxidant molecules that can play an important role in the pathogenesis of iron-deficiency anemia (IDA). The aim of this study was to investigate oxidative status in patients with IDA and alteration of oxidative status after iron treatment. Thirty-three female patients with IDA and 25 healthy controls were included in this study. Oxidant and total antioxidant capacity were determined using free oxygen radicals test and free oxygen radicals defence (Form CR 3000, Callegari, Parma, Italy). Catalase activity was measured by spectrophotometer using a commercially ava...
Source: Journal of Clinical Laboratory Analysis - September 21, 2009 Category: Laboratory Medicine Authors: Jong-Ha Yoo, Ho-Young Maeng, Young-Kyu Sun, Young-Ah Kim, Dong-Wook Park, Tae Sung Park, Seung Tae Lee, Jong-Rak Choi Tags: Original Articles Source Type: journals

An improved microalbumin method (µALB_2) with extended analytical measurement range evaluated on the ADVIA® chemistry systemsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Quantitative determination of albumin (ALB) in human urine is important to assess kidney functions in a variety of diseases. Recently, Siemens released an improved Microalbumin assay (µALB_2) to measure urinary ALB on the automated, random access ADVIA 1650/1800, ADVIA 2400, and ADVIA 1200 Chemistry Systems. We evaluated analytical performances of this new method. All ADVIA Chemistry Systems use the same microalbumin reagent packs, µALB_2 calibrators, and commercial controls. The within-run and total CVs of the improved method with two-level BioRad Liquichek Urine Chemistry controls ([sim]2 and 9 mg/dl ALB) and a urine p...
Source: Journal of Clinical Laboratory Analysis - September 21, 2009 Category: Laboratory Medicine Authors: Pradip Datta, Amitava Dasgupta Tags: Original Articles Source Type: journals

Systematic analysis of interference due to stutter in estimating chimerism following hematopoietic cell transplantationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a mathematical approach to minimize the contribution of stutter when estimating chimerism following HCT. Pretransplant DNA samples from 409 donors and recipients were used to define marker-specific stutter cut-off values for all makers used in the AMPF[ell]STR Profiler Plus amplification kit. Mock chimerism samples (5, 20, and 50%) were used to evaluate the contribution of stutter in estimating chimerism. Three markers, vWA, D13S317, and D18S51, had overlapping stutter in the mock chimerism samples. Only D18S51 had stutter from a shared allele, whereas the other two markers had stutter from nonshared alleles. W...
Source: Journal of Clinical Laboratory Analysis - September 21, 2009 Category: Laboratory Medicine Authors: Bharat Thyagarajan, Shawn Young, Stacy Floodman, Randolph Peterson, Xinjing Wang Tags: Original Articles Source Type: journals

Expression of CD4+CD25highCD127low/- regulatory T cells in transitional cell carcinoma patients and its significanceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To evaluate the expressions of CD4+CD25highCD127low/- regulatory T cells (Tregs) in peripheral blood from patients with transitional cell carcinoma (TCC) in urinary system, we investigated the proportion of Treg population in CD4+ T from 93 patients with TCC, 38 with benign urinary diseases, and 37 healthy subjects by using flow cytometric analysis and analyzing different clinicopathologic characteristics and the changes before and after operation. We found that the proportion of Treg in peripheral blood from patients with TCC was significantly increased as compared with the othertwo groups. There was a strong correlation ...
Source: Journal of Clinical Laboratory Analysis - July 22, 2009 Category: Laboratory Medicine Authors: Xi Zhu, Lin-Lin Ma, Tian Ye Tags: Original Articles Source Type: journals

Immunohistochemical expression of MAP1LC3A and MAP1LC3B protein in breast carcinoma tissuesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Autophagy is a protein degradation process within the cell and its deregulation has been linked to various diseases and the formation of cancer. One of the important proteins involved in the autophagy process is microtubule-associated protein 1 light chain 3 (MAP1LC3). The aims of this study were to determine the MAP1LC3A and MAP1LC3B protein expression in both normal and cancer breast tissues and to determine the relationship between the expression of these proteins and type of tissues. Immunohistochemistry assessments were carried out on tissue microarrays consisting of breast tissues. MAP1LC3A expression was detected in...
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: Ekhlas Qaid Gazem Othman, Gurjeet Kaur, Ahmad Faisal Mutee, Tengku Sifzizul Tengku Muhammad, Mei Lan Tan Tags: Original Articles Source Type: journals

Cancer antigen 125 levels in inflammatory bowel diseasesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our data suggest that serum CA-125 levels may be increased in patients with IBDs. J. Clin. Lab. Anal. 23:244-248, 2009. © 2009 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: Hilmi Ataseven, Zeynel Abidin Öztürk, Mehmet Arhan, Osman Yüksel, Seyfettin Köklü, Mehmet [Idot]bi[scedil], Ömer Ba[scedil]ar, Fatma Meriç Y[inodot]lmaz, [Idot]lhami Yüksel Tags: Original Articles Source Type: journals

Association of [beta]-1 and [beta]-2 adrenergic receptor gene polymorphisms with myocardial infarctionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we aimed to investigate the possible relationship between Ser49Gly and Arg389Gly polymorphisms of the ADRB1 and Arg16Gly and Gln27Glu polymorphisms of the ADRB2 gene with ST elevation myocardial infarction (MI) in a Turkish population. One hundred patients with ST elevation MI and 100 healthy control subjects were genotyped using the PCR-RFLP method. Although the Arg389 allele of the ADRB1 gene was associated with an elevated risk of MI, the Glu27 allele of the ADRB2 gene was associated with a decreased risk of MI. Carriers of the ADRB1 Arg389 allele (heterozygotes+homozygotes) had an approximately 3.5-fold ...
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: Akin Yilmaz, Mehmet G. Kaya, Ulgen Merdanoglu, Mehmet A. Ergun, Atiye Cengel, Sevda Menevse Tags: Original Articles Source Type: journals

Quantitative risk estimation for large for gestational age using the area under the 100-g oral glucose tolerance test curveEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We devised a complementary quantitative method for gestational diabetes (GDM) that uses the area under the curve (AUC) of the results of the oral glucose tolerance test (OGTT), and evaluated its efficacy in predicting neonates that would be large for gestational age (LGA). The study subjects were 648 pregnant women. The AUC-OGTT (concentration × time) was calculated from the 100-g OGTT results. The incidence of LGA according to each range of the AUC-OGTT was estimated and odds ratios were analyzed using multiple logistic regression analysis.The incidence of LGA increased with the AUC-OGTT value and was 0% for AUC (Source:...
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: Sollip Kim, Won-Ki Min, Sail Chun, Woochang Lee, Hee-Jung Chung, Pil Ryang Lee, Ahm Kim Tags: Original Articles Source Type: journals

Quantitative detection of PML-RAR[alpha] fusion transcript by real-time PCR with a single primer pairEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Quantitative detection of minimal residual disease has prognostic value for some leukemias. Acute promyelocytic leukemia (APL) is characterized by the specific PML-RAR[alpha] fusion gene from t(15;17). Added to three PML-RAR[alpha] isoforms, alternative spliced forms of PML exons give rise to multiple isoforms even within a single patient. To date, multiple primer pairs for the detection of the various PML-RAR[alpha] transcripts have been designed, potentially generating some nonspecific amplification products. Here, we established a real-time quantitative PCR (RQ-PCR) strategy with a single primer pair using LightCycler (...
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: Mariko Takenokuchi, Yuji Nakamachi, Keiko Yoneda, Kana Joo, Seiji Kawano, Eiji Tatsumi, Katsuyasu Saigo, Shunichi Kumagai Tags: Original Articles Source Type: journals

Helicobacter pylori seropositivity and its relationship with serum malondialdehyde and lipid profile in preeclampsiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study included 53 preeclamptic and 30 healthy pregnants. Serum levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C), MDA, and H. pylori Ig G antibodies were measured. Results: Preeclampsia group had higher serum MDA levels compared with healthy pregnants (P (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: Hulya Aksoy, Asuman Ozkan, Ferda Aktas, Bunyamin Borekci Tags: Original Articles Source Type: journals

Clinical evaluation of serum alpha-fetoprotein-IgM immune complexes on the diagnosis of primary hepatocellular carcinomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We evaluated clinical significance of serum alpha-fetoprotein (AFP)-IgM immune complexes, in comparison with free AFP, on the diagnosis of primary hepatocellular carcinoma (HCC). Serum levels of AFP-IgM immune complexes and free AFP were determined by the ELISA method and electrochemiluminescence, respectively, in 103 healthy controls, 74 patients suffering from primary HCC, 27 patients suffering from liver cirrhosis, and 63 patients suffering from chronic hepatitis. The best cut-off value of AFP-IgM and free AFP for diagnosis of primary HCC were 300 AU/mL and 10 µg/L respectively, according to the area under the curve (A...
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: Jiang Jingting, Wu Changping, Xu Ning, Zhu yibei, Wu Jun, Ji Mei, Xu Bin, Nilsson-Ehle Peter, Zhang Xueguan Tags: Original Articles Source Type: journals

Anticardiolipin antibodies in the sera of patients with diagnosed chronic fatigue syndromeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Examination of anticardiolipin antibodies (ACAs) in the sera of patients clinically diagnosed with chronic fatigue syndrome (CFS) using an enzyme-linked immunoassay procedure demonstrated the presence of immunoglobulin M isotypes in 95% of CFS serum samples tested. The presence of immunoglobulin G and immunoglobulin A isotypes were also detected in a subset of the samples. Future studies will focus on elucidating whether alterations to mitochondrial inner membranes and/or metabolic functions play a possible role in the expression of ACAs. J. Clin. Lab. Anal. 23:210-212, 2009. © 2009 Wiley-Liss, Inc. (Source: Journal of Cl...
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: Yoshitsugi Hokama, Cara Empey Campora, Cynthia Hara, Tina Kuribayashi, Diana Le Huynh, Kenichi Yabusaki Tags: Original Articles Source Type: journals

Simultaneous use of direct and indirect diagnostic techniques in atypical respiratory infections from Chlamydophila pneumoniae and Mycoplasma pneumoniaeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In 2008, 50 samples (BAL), coming from hospital patients, with acute respiratory symptoms have been investigated using two real-time PCR methods: one assay for the single detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae DNA and one commercially available real-time duplex PCR assay for the detection of C. pneumoniae and M. pneumoniae DNA. Both techniques used here showed compliant results, with 100% concordance for detection of C. pneumoniae and 98% for detection of M. pneumoniae. The positive results obtained agreed with the clinical suspicion of such infections in some cases and with the presence of IgM spe...
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: S. Pignanelli, A. Shurdhi, F. Delucca, M. Donati Tags: Original Articles Source Type: journals

Improvement of the newly developed latex agglutination test (Katex) for diagnosis of visceral lieshmaniasisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: These data indicate that DTT can be used to eliminate nonspecific reactivity in the Katex. This will improve the performance of Katex and make it a more convenient and field applicable test. J. Clin. Lab. Anal. 23:202-205, 2009. © 2009 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - July 20, 2009 Category: Laboratory Medicine Authors: Gholam Reza Hatam, Mohammad Amin Ghatee, Seyed Mohammad Hossein Hossini, Bahador Sarkari Tags: Original Articles Source Type: journals

Checklist for optimization and validation of real-time PCR assaysEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Real-time polymerase chain reaction (PCR) is a frequently used technique in molecular diagnostics. To date, practical guidelines for the complete process of optimization and validation of commercial and in-house developed molecular diagnostic methods are scare. Therefore, we propose a practical guiding principle for the optimization and validation of real-time PCR assays. Based on literature, existing guidelines, and personal experience, we created a checklist that can be used in different steps of the development and validation process of commercial and in-house developed real-time PCR assays. Furthermore, determination o...
Source: Journal of Clinical Laboratory Analysis - May 20, 2009 Category: Laboratory Medicine Authors: Marijke Raymaekers, Rita Smets, Brigitte Maes, Reinoud Cartuyvels Tags: Original Articles Source Type: journals

Determining urinary trace elements (Cu, Zn, Pb, As, and Se) in patients with bladder cancerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Objectives: Trace elements are essential components of biological structures, but they can be toxic at concentrations beyond those necessary for their biological functions.Methods: A study group of 30 patients with bladder cancer and a control group of 30 healthy volunteers were measured for trace elements using a graphite furnace atomic absorption spectrophotometer.Results: Urinary zinc and selenium levels in patients were significantly (P (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - May 19, 2009 Category: Laboratory Medicine Authors: Chang-Ni Lin, Lai-Hao Wang, Kun-Hung Shen Tags: Original Articles Source Type: journals

Filarial infection is resisted differentially by subjects having different blood group phenotypesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was aimed to elucidate the relationship between major blood group antigens (BGAs) and susceptibility or resistance to human lymphatic filariasis. A total of 492 human subjects, living in Raipur city of Chhattisgarh, endemic for bancroftian filariasis in Central India, were screened for the presence of Wuchereria bancrofti microfilariae and disease manifestations. The frequency of BGAs was tabulated as a function of the status of filariasis, namely normal (no infection), mf carrier (presence of microfilariae in blood), and elephantiasis (confirmed clinical manifestations). The comparison of ABO phenotype distribu...
Source: Journal of Clinical Laboratory Analysis - May 19, 2009 Category: Laboratory Medicine Authors: Vandana Dixit, A.K. Pati, A.K. Gupta, P.S. Bisen, G.B.K.S. Prasad Tags: Original Articles Source Type: journals

Simultaneous determination of [alpha]-fetoprotein immune complexes and [alpha]-fetoprotein concentration in hepatocellular carcinoma using dual-label time-resolved immunofluorometric assaysEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was aimed at developing a novel time-resolved immunofluorometric assay (TR-IFMA) for the simultaneous determination of AFP-IgM and AFP concentration in HCC. We constructed a double-label assay by using Sm3+-labeled mAb to human IgM antibody, Eu3+-labeled mAb to AFP, and immobilized another mAb to AFP on the solid phase. The performances of the assay were all found to be satisfactory. The validity of the novel assay was confirmed by the good correlation between the results obtained by TR-IFMA and commercial ELISA or electrochemiluminescence immunoassay. AFP-IgM and AFP were increased above the cutoffs in 65.25 an...
Source: Journal of Clinical Laboratory Analysis - May 19, 2009 Category: Laboratory Medicine Authors: Shi Le Sheng, Qing Wang, Gang Huang, Bin Yu, Wen Xin Qin Tags: Original Articles Source Type: journals

Age-dependent decreases in serum soluble interleukin-1 receptor type I (sIL-1RI) in healthy individuals: a population study of serum sIL-1RI levels in Japanese subjectsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The levels of several soluble cytokine receptors in body fluids of healthy individuals change with age. Clinical application of the measurement of the serum soluble interleukin-1 receptor type I (sIL-1RI) level depends critically on the samples used as the controls. At present, there is no information regarding the levels of serum sIL-1RI in healthy subjects. The purpose of this study is to reveal the age-related changes that occur in the serum sIL-1RIlevels of healthy individuals. We determined the serum sIL-1RI levels of healthy Japanese children using ELISA. The serum sIL-1RI level of children (0-14 years) was significa...
Source: Journal of Clinical Laboratory Analysis - May 19, 2009 Category: Laboratory Medicine Authors: Yoshihiro Okamoto, Mayuri Tanaka, Nobuhiro Miyahara, Mie Tanabe, Yoshimitsu Gotoh, Takuhito Nagai, Naoya Fujita, Takashi Fukui, Toshiyuki Masuzawa Tags: Original Articles Source Type: journals

Simplified and versatile method for bisulfite-based DNA methylation analysis of small amounts of DNAEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Epigenetic alterations of gene function play a central role in the pathogenesis of many tumors and in the process of aging. Abnormal methylation at transcriptional sites of genes results in epigenetic silencing of the genes that protect against tumor formation or that repair DNA. To date, several studies have analyzed methylation status by oligonucleotide arrays, restriction analysis (COBRA), methylation-specific amplification, and sequence analysis. Thisrequires high concentration of bisulfite-treated DNA, which mandates use of commercially available expensive kits, and is an often laborious and time-consuming task. In th...
Source: Journal of Clinical Laboratory Analysis - May 19, 2009 Category: Laboratory Medicine Authors: S.K. Tiwari, G. Manoj, K. Prasanth, G. Sivaram, V.K. Sharma, M.A. Habeeb, A.A. Khan, C.M. Habibullah Tags: Original Articles Source Type: journals

Diagnostic efficacy of the ELISA test for the detection of deamidated anti-gliadin peptide antibodies in the diagnosis and monitoring of celiac diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The very high specificity of the AGAs IgG test (99.2%) also suggests that patients who test positive with this assay require a thorough followup, even if the anti-tissue transglutaminase antibodies (anti-tTG) and anti-endomysial autoantibodies (EMA) assays are negative. J. Clin. Lab. Anal. 23:165-171, 2009. © 2009 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - May 19, 2009 Category: Laboratory Medicine Authors: Elio Tonutti, Daniela Visentini, Alessia Picierno, Nicola Bizzaro, Danilo Villalta, Renato Tozzoli, Graziano Kodermaz, Antonio Carroccio, Giuseppe Iacono, Saverio Teresi, Stella Maria La Chiusa, Ignazio Brusca Tags: Original Articles Source Type: journals

Evaluation of extracts of Jatropha curcas and Moringa oleifera in culture media for selective inhibition of saprophytic fungal contaminantsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Most fungi occur in nature and utilize simple sources of carbohydrates and nitrogen for growth. Sabouraud's dextrose agar has been an ideal medium for primary isolation of fungi from clinical specimens, but for specimens from nonsterile sites or heavily contaminated ones, it has been necessary to include inhibitory substances such as antibiotics like chloramphenicol (antibacterial) and cycloheximide (antifungal). The problems we have in the our laboratory owing to frequent contamination of cultures and the delays in the procurement of cycloheximide have stimulated a search for alternatives in our local environment to enhan...
Source: Journal of Clinical Laboratory Analysis - May 19, 2009 Category: Laboratory Medicine Authors: Grace Mebi Ayanbimpe, Titilayo K. Ojo, Ezekiel Afolabi, Faith Opara, Sylvia Orsaah, Olalekan Stephen Ojerinde Tags: Original Articles Source Type: journals

Binding of anti-HLA class I antibody to endothelial cells produce an inflammatory cytokine secretory patternEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Current methods are inadequate for the diagnosis of early chronic allograft rejection. The goal of this study was to determine whether ligation of anti-HLA antibodies to endothelial cells is associated with a distinctive cytokine secretory pattern. Human iliac artery endothelial cells (HIAEC) cultured in vitro were incubated with w6/32, an anti-HLA class I mAb. Culture supernatants collected daily for up to 4 days were tested for secretion of 13 cytokines using a multiplexed fluorescent microsphere immunoassay. Culture of HIAEC with medium containing mAb w6/32 supported the growth of HIAEC during the 4-day study period. Le...
Source: Journal of Clinical Laboratory Analysis - May 19, 2009 Category: Laboratory Medicine Authors: Eduardo Reyes-Vargas, Igor Y. Pavlov, Thomas B. Martins, Jason J. Schwartz, Harry R. Hill, Julio C. Delgado Tags: Original Articles Source Type: journals

Comparative study of amplification systems in immunoenzyme assays for the diagnosis of American tegumentary leishmaniasisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We compared the accuracy and reliability of three amplification systems for enzyme immunoassays in the detection of specific IgG antibodies for the diagnosis of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in patients from an endemic area in Rio de Janeiro, Brazil. Partially soluble antigens obtained from the promastigote forms of L. (V.) braziliensis were used. For development of the reaction, two chromogens, 1,2-orthophenylenediamine (OPD) and 3,3[prime],5,5[prime]-tetramethylbenzidine (TMB), and a fluorogen, 4-methylumbelliferylphosphate (MUP), were tested. The performance of each system was compa...
Source: Journal of Clinical Laboratory Analysis - May 19, 2009 Category: Laboratory Medicine Authors: Lílian Dias Nascimento, Sonia Regina Lambert Passos, Eliame Mouta-Confort, Marta de Almeida Santiago, Andreia Silva Alves, Maria de Fátima Madeira, Armando de Oliveira Schubach, Mauro Célio de Almeida Marzochi Tags: Original Articles Source Type: journals

Interleukin-18 gene 105A/C genetic polymorphism is associated with the susceptibility of Kawasaki diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Interleukin-18 (IL-18)-656T/G, -607A/C, and -137C/G promoter polymorphisms had been reported associated with Kawasaki disease (KD). An IL-18 genetic A/C polymorphism at coding position 105 (rs549908) has been linked with asthma, rheumatoid, and systemic lupus erythematosus. We tested a hypothesis that the IL-18 105A/C genetic polymorphism confers KD susceptibility. Study participants were Taiwanese KD patients and a healthy control group. Our data indicated that the frequency of C allele was significantly higher in the patient group (13.9%) than in the control group (2.7%; P (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - March 14, 2009 Category: Laboratory Medicine Authors: Shih-Yin Chen, Lei Wan, Yu-Chuen Huang, Jim Jinn-Chyuan Sheu, Yu-Ching Lan, Chih-Ho Lai, Cheng-Wen Lin, Jeng Sheng Chang, Yuhsin Tsai, Shih-Ping Liu, Ying-Ju Lin, Fuu-Jen Tsai Tags: Original Articles Source Type: journals

Detection of Paracoccidioides brasiliensis gp43 Gene in Sputa by Loop-Mediated Isothermal Amplification MethodEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The fungus Paracoccidioides brasiliensis is the pathogen of paracoccidioidomycosis (PCM), a systemic mycosis prevalent in Latin America. The loop-mediated isothermal amplification method (LAMP) was used in this study to detect the presence of P. brasiliensis in sputa samples from patients with chronic PCM, suspected PCM, and a negative control. The target P. brasiliensis gp43 gene was amplified in less than 4 hr in 11 of 18 sputa samples tested. The LAMPmethod had the advantage of speed and simplicity compared with the classic diagnostic methods such as the histopathological test or biological material culture and did not ...
Source: Journal of Clinical Laboratory Analysis - March 13, 2009 Category: Laboratory Medicine Authors: B. T. Tatibana, A. Sano, J. Uno, K. Kamei, T. Igarashi, Y. Mikami, M. Miyaji, K. Nishimura, E. N. Itano Tags: Original Articles Source Type: journals

Serum prolidase activity and oxidative status in patients with bronchial asthmaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Asthma is a disease where there is an accumulation of collagen in the reticular basal membrane of the airway leading to chronic inflammation. The enzyme prolidase plays an important role in the breakdown of collagen and the breakdown of intracellular protein especially in the final stage when peptides and dipeptides contain a high level of proline. To evaluate the relationship between prolidase activity and oxidative status in asthma patients. Comparison was made between 42 patients diagnosed with bronchial asthma and 32 healthy children of similar age and gender. Serum prolidase activity was measured spectrophotometricall...
Source: Journal of Clinical Laboratory Analysis - March 13, 2009 Category: Laboratory Medicine Authors: Alpay Cakmak, Dost Zeyrek, Ali Atas, Hakim Celik, Nurten Aksoy, Ozcan Erel Tags: Original Articles Source Type: journals

Associations of IgG N-linked oligosaccharide chains and proteases in sera of prostate cancer patients with and without [alpha]2-macroglobulin deficiencyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We previously reported on a number of cases of metastatic prostate cancer (PCa) in which serum [alpha]2-macroglobulin ([alpha]2M) levels were markedly decreased to less than 20 mg/dl ([alpha]2M deficiency). All PCa patients with [alpha]2M deficiency had multiple bone metastases. Proteases in ten PCa patients with and without [alpha]2M deficiency were studied and compared against ten healthy controls in order to elucidate the relationships between changes in sugar chain structure and neoplasia. We assessed the relationship between ratios of Fr4 to Fr1 and Fr2 (Fr4/Fr1+Fr2 ratios) of oligosaccharide chains, and ratios of fre...
Source: Journal of Clinical Laboratory Analysis - March 13, 2009 Category: Laboratory Medicine Authors: Yuhsaku Kanoh, Shin Egawa, Shiro Baba, Tohru Akahoshi Tags: Original Articles Source Type: journals

Novel endotoxin assay by laser light-scattering particle-counting methodEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Exposure of Limulus amoebocyte lysate to endotoxin under stirring produced light-reflective particles that appeared to be coagulin polymers. A laser light-scattering particle counter, the PA-200, detected these particles sensitively. The PA-200 detected endotoxin at a concentration as low as 0.00015 EU/ml in 71 min, whereas the minimum endotoxin concentration measured by a turbidimeter, ET-2000, was 0.0005 EU/ml in 138 min. Moreover, PA-200 was much less affected by the presence of colored substances and refractive materials than was ET-2000. We propose that the high sensitivity, speed, and high interference tolerance of t...
Source: Journal of Clinical Laboratory Analysis - March 13, 2009 Category: Laboratory Medicine Authors: Kotaro Mitsumoto, Katsumi Yabusaki, Koji Kobayashi, Yoshiaki Shirasawa, Toru Obata Tags: Original Articles Source Type: journals

Detection of C1236T, G2677T/A, and C3435T polymorphism of MDR1 by amplification refractory mutation system PCREmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
C1236T, G2677T/A, and C3435T polymorphism of the multidrug resistance (MDR1) gene have substantial impact on expression or activity of P-glycoprotein (P-gp). We developed new methods based on amplification refractory mutation system (ARMS) to detect these polymorphisms. Tetra-primers amplification in a single tube was established to detect C1236T and C3435T polymorphism. For G2677T/A polymorphism, a two-step allele-specific amplification method was used. MDR1 genotypes of 177 Chinese subjects were determined by the methods we established. The methods we established were verified with gene sequencing. Gene frequencies of 12...
Source: Journal of Clinical Laboratory Analysis - March 13, 2009 Category: Laboratory Medicine Authors: Bing Chen, Jie Fang, Weixia Zhang, Zhao Jin, Zichen Yu, Weimin Cai Tags: Original Articles Source Type: journals

Serum lipid profile, oxidative status, and paraoxonase 1 activity in hyperemesis gravidarumEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to investigate lipid profile, paraoxonase 1 (PON1) activity, and oxidative stress status in the serum of hyperemesis gravidarum (HG) patients. Thirty-six HG cases and 36 normal pregnants were included in the study. Serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apoproteins A1 (apo A1) and B (apo B), malondialdehyde (MDA), and total antioxidant activity (TAO) values and PON1 and arylesterase activities were determined. Although serum TC, TG, LDL-C, and apo B levels were not different among; the groups (P>0....
Source: Journal of Clinical Laboratory Analysis - March 13, 2009 Category: Laboratory Medicine Authors: Hulya Aksoy, Ayse Nur Aksoy, Asuman Ozkan, Harun Polat Tags: Original Articles Source Type: journals

Lipid peroxidation and antioxidant enzyme activities in infertile men: correlation with semen parameterEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to investigate the oxidative stress status and antioxidant enzyme activities in infertile men's semen and to determine their relationship with spermatozoa characteristics. Four groups of infertile men, normozoospermic (n=9), azoospermic (n=13), oligoasthenozoospermic (n=38), and asthenozoospermic (n=42), were tested for malonaldialdehyde (MDA) concentration and catalase (CAT) and superoxide dismutase (SOD) activities in semen using spectrophotometric methods. We found that MDA levels in semen and SOD activity in seminal plasma (SP) were significantly higher in oligoasthenozoospermic and asthenozoo...
Source: Journal of Clinical Laboratory Analysis - March 13, 2009 Category: Laboratory Medicine Authors: Fatma Ben Abdallah, Ines Dammak, Hamadi Attia, Basma Hentati, Leila Ammar-Keskes Tags: Original Articles Source Type: journals

Effect of sample storage on stability of salivary glutathione, lipid peroxidation levels, and tissue factor activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Saliva samples are often required to be stored for longer periods of time either because of the project protocol or because of lack of funding for analysis. The effects of 6 months storage (fresh, 30, 60, 90 120, 150, and 180 d) on the stability of salivary reduced glutathione (GSH), lipid peroxidation (LPO) and 90 days of storage (fresh, 15, 30, 60, and 90 d) on the stability of salivary tissue factor (TF) activity and the stability of saliva imprint samples at -20°C were evaluated in this study. Salivary GSH, malondialdehyde (MDA) levels as an index of LPO, and TF activities were determined using the methods of Beutler,...
Source: Journal of Clinical Laboratory Analysis - March 13, 2009 Category: Laboratory Medicine Authors: Ebru Emekli-Alturfan, Emel Kasikci, A. Ata Alturfan, Rabia Pisiriciler, Aysen Yarat Tags: Original Articles Source Type: journals

Evaluation of metalloproteinases 2 and 9 and their inhibitors in physiologic and pre-eclamptic pregnancyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Matrix metalloproteinases (MMPs) are a family of zinc and calcium-dependent endopeptidases involved in remodeling and physiological homeostasis of extracellular matrix (ECM). The metalloproteinases activity is predominantly modulated by specific tissue inhibitors of matrix metalloproteinases (TIMPs). The balance between MMPs and TIMPs is likely to play an important role in remodeling uterine arteries in pregnancy, and it may represent means by which vasodilatation is maintained in later pregnancy. Moreover, increased levels of MMPs and in particular MMP-2 play a role in the vascular alterations induced by hypertension. The...
Source: Journal of Clinical Laboratory Analysis - March 13, 2009 Category: Laboratory Medicine Authors: Martina Montagnana, Giuseppe Lippi, Alessandro Albiero, Silvia Scevarolli, Gian Luca Salvagno, Massimo Franchi, Gian Cesare Guidi Tags: Original Articles Source Type: journals