HPV genotyping and E6/E7 transcript assays for cervical lesion detection in an Asian Screening Population – Cobas and Aptima HPV tests
Persistent hrHPV infection has been established as a necessary oncogenic factor for cervical carcinogenesis [1]. Globally, cervical cancer remains one of the most deadly malignancies in women but its incidence is highly reducible by screening, HPV vaccination and education. Screening for cancerous and precancerous cervical lesions has been performed through morphological examination of cervical cytology (pap smear). Detection of hrHPV in cervical sample is emerging as the molecular test for cervical cancer screening and for triage of cases with equivocal pap smear. (Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - October 13, 2018 Category: Virology Authors: Oscar G.W. Wong, Obe K.L. Tsun, Eva Y Tsui, Joanne NK Chow, Philip PC Ip, Annie NY Cheung Source Type: research
Evaluation of performance characteristics of hepatitis B e antigen assays
Approximately 240 million people worldwide are chronically infected with hepatitis B virus (HBV) [1], with about 700,000 deaths from complications of HBV infection, including cirrhosis and liver cancer [2]. HBV infection is determined through serological testing for HBV surface antigen (HBsAg), and additional serology tests alone or in combination can establish acute, chronic and resolved HBV infection. HBV e antigen (HBeAg) is associated with active HBV replication and increased risk for hepatocellular carcinoma (HCC), regardless of levels of alanine aminotransferase (ALT) and stage of liver disease [3 –5]. (Source: Jou...
Source: Journal of Clinical Virology - October 13, 2018 Category: Virology Authors: Tonya Mixson-Hayden, Michael A. Purdy, Lilia Ganova-Raeva, Daniel McGovern, Joseph C. Forbi, Saleem Kamili Source Type: research
Risk factors for BK virus viremia and nephropathy after kidney transplantation: a systematic review
In recent years, BK polyomavirus (BKPyV) infection has emerged as a major problem in kidney transplant recipients. About 90% of the latter are seropositive for BKPyV [1]. The virus reactivates periodically with urinary shedding but remains asymptomatic in immunocompetent adults. The immunosuppressive therapy used to prevent rejection after solid organ transplantation allows viral infections to emerge. In particular, BK virus can reactivate after kidney transplantation, and may induce progressive disease in three successive stages: viruria, viremia, and then (if viral replication persists) nephropathy. (Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - October 12, 2018 Category: Virology Authors: Baptiste Demey, Claire Tinez, Catherine Fran çois, François Helle, Gabriel Choukroun, Gilles Duverlie, Sandrine Castelain, Etienne Brochot Source Type: research
Performance of a commercial assay for detecting and quantifying HEV RNA in faeces
Over the last 10 years, it has become apparent that hepatitis E virus (HEV) is a pathogen of global significance. HEV infection is among the most frequent causes of acute hepatitis worldwide (1). The HEV strains infecting humans are classified as one of 4 major genotypes. Peak viremia occurs during the incubation period and early phase of disease In patients with an acute HEV infection (2). Viral RNA can be detected in the blood and faeces of these patients just before the onset of clinical symptoms. (Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - October 10, 2018 Category: Virology Authors: Florence Abravanel, Audrey Lacipi ère, Sébastien Lhomme, Martine Dubois, Luce Minier, Jean-Marie Peron, Laurent Alric, Nassim Kamar, Jacques Izopet Source Type: research
Biological relevance of Cytomegalovirus genetic variability in congenitally and postnatally infected children
Human cytomegalovirus (HCMV), a double stranded DNA herpesvirus, is the most frequent cause of congenital malformations worldwide, resulting in neurodevelopmental delay, foetal or neonatal death, and most frequently sensorineural hearing loss [1 –3]. It is characterized by a large genome encoding a wide range of gene products, endowed of effective immunomodulatory activity [4–6]. For instance, different viral genes encoding tumor necrosis factor-alpha (TNF-α) receptor (UL144), α-chemokines (UL146-147), β-chemokine receptor (US28) are potential virulence factors associated with severe congenital HCMV infection [7,8]....
Source: Journal of Clinical Virology - October 3, 2018 Category: Virology Authors: Ganna Galitska, Matteo Biolatti, Marco De Andrea, Agata Leone, Alessandra Coscia, Luigi Bertolotti, Ugo Ala, Enrico Bertino, Valentina Dell ’Oste, Santo Landolfo Source Type: research
CD4 T cell responses to flaviviruses
Flaviviruses are typically transmitted to humans by infected mosquitoes or ticks. The most important human-pathogenic representatives are yellow fever (YF), Japanese encephalitis (JE), West Nile (WN), dengue (DEN), Zika and tick-borne encephalitis (TBE) viruses, which constitute significant health problems in many parts of the world. The expansion of flavivirus endemic areas and their ability to cause epidemics are most dramatically exemplified by the expansion of DEN hyperendemic areas [1], the expansion of WN virus in the Americas after its introduction to New York in 1999 [2] and its emergence in a number of countries o...
Source: Journal of Clinical Virology - October 3, 2018 Category: Virology Authors: Judith H. Aberle, Maximilian Koblischke, Karin Stiasny Source Type: research
Neonatal HCMV-related polymicrogyria in seroimmune women: what is the optimal pregnancy management?
Congenital HCMV is the most common congenital infection in developed countries with vertical transmission occurring following both primary (32%) and non-primary (1.4%) maternal infection. [1]. In the latter, whether congenital infection is a consequence of maternal reactivation of the latent HCMV strain(s), or of maternal reinfection with a new strain, is an issue that remains undefined. However, recent studies have argued that maternal super-infection with a serologically distinct HCMV strain could play a significant role in congenital HCMV infection [2]. (Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - October 3, 2018 Category: Virology Authors: Maurizio Zavattoni, Giuseppina Lombardi, Francesca Garofoli, Guido Scalia, Annalisa Rizzo, Micol Angelini, Antonella Sarasini, Milena Furione, Fausto Baldanti Source Type: research
Recognition, treatment, and sequelae of congenital cytomegalovirus in Australia: An observational study
Congenital CMV (cCMV) has an overall birth prevalence of 0.6-0.7%, with approximately 10-15% symptomatic during the newborn period [1,2]. Both symptomatic and asymptomatic newborns remain at risk of long-term sequelae, particularly hearing loss and developmental delay [2]. The mainstay of long-term management remains supportive hearing and developmental interventions [3], with evidence supporting a role for antiviral therapy commenced during the newborn period for infants with symptomatic cCMV [4,5]. (Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - September 27, 2018 Category: Virology Authors: Adam W Bartlett, Beverley M Hall, Pamela Palasanthiran, Brendan McMullan, Antonia W Shand, William D Rawlinson Source Type: research
Evolution of symptoms and quality of life during Zika virus infection: a 1-year prospective cohort study
We present a 1-year follow-up of clinic, biology and quality of life from 49 ZIKV infected volunteers in French Guiana (diagnosis confirmed by RT-PCR in serum or urine). (Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - September 26, 2018 Category: Virology Authors: Franck de Laval, Hubert d ’Aubigny, Séverine Mathéus, Thomas Labrousse, Anne Laure Ensargueix, Enguerrane Martinez Lorenzi, François Xavier Le Flem, Natalie André, Didier Belleoud, Dominique Rousset, Fabrice Simon, Sébastien Briolant Source Type: research
Editorial Board
(Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - September 19, 2018 Category: Virology Source Type: research
ESCV Membership
(Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - September 19, 2018 Category: Virology Source Type: research
PASCV Membership
(Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - September 19, 2018 Category: Virology Source Type: research
Within-Host Evolution of Virus Variants during Chronic Infection with Novel GII.P26-GII.26 Norovirus
Noroviruses, a genus of Caliciviridae, are small non-enveloped viruses and a leading cause of acute gastroenteritis [1]. They are easily spread by the fecal-oral route, as viral particles are highly contagious and largely unaffected by cold and heat inactivation, or exposure to detergent-based cleaners [2]. Norovirus infections cause a rapidly self-limiting illness with vomiting and diarrhea. Mainly elderly, infant and immunocompromised patients tend to suffer from severe disease [3]. Especially in the immunocompromised, chronic norovirus infections occur, associated with persistent shedding of infectious norovirus in stoo...
Source: Journal of Clinical Virology - September 19, 2018 Category: Virology Authors: Corinna Pietsch, Nora Ennuschat, Sabine H ärtel, Uwe G. Liebert Source Type: research
Evaluation of the vitros hiv combo 4th generation test for the identification of hiv infections
Antibody detection against Human Immunodeficiency Virus (HIV) is a commonly-used method for screening HIV infection. Over time, with the improvement of immunological techniques, several generations of tests for detection of anti-HIV antibodies have been developed. The first generation tests, which used an HIV lysate as the source of antigens for the test preparation, had some specificity problems that were minimized in second generation tests which used recombinant antigens (eg in Escherichia coli). (Source: Journal of Clinical Virology)
Source: Journal of Clinical Virology - September 17, 2018 Category: Virology Authors: Massimo De Paschale, Maria Teresa Manco, Annalisa Cianflone, Luisa Belvisi, Debora Cagnin, Teresa Cerulli, Alessia Paganini, Carlo Agrappi, Paola Mirri, Pierangelo Clerici Source Type: research
Analytical and field evaluation of the Biocentric Generic HCV Assay on open polyvalent PCR platforms in France and Cambodia
Treatment options to achieve definitive cure of chronic hepatitis C infection have been recently revolutionised with the second generation of direct-acting antivirals (DAAs). The HCV global strategy launched in 2016 by World Health Organisation (WHO) aimed to eliminate HCV epidemic by 2030[1]. Reducing the burden of HCV in low- and middle-incomes countries necessitates ongoing efforts to improve access to treatment and diagnosis. The expansion in affordable DAAs availability, creates an urgent need for scale-up and strengthening of the laboratory equipment required for initiation and monitoring of HCV treatment. (Source: J...
Source: Journal of Clinical Virology - September 17, 2018 Category: Virology Authors: J Nouhin, K Bollore, J Castera-Guy, S Prak, S Heng, A Kerleguer, PA Rubbo, F Rouet, E Tuaillon Source Type: research
