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69 records returned

An analysis of levels of infectious pancreatic necrosis virus in Atlantic salmon, Salmo salar L., broodstock in Scotland between 1990–2002Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Throughout this study period the prevalence of infectious pancreatic necrosis virus (IPNV) in Scottish farmed Atlantic salmon was high in the marine environment but relatively low in fresh water. In order to minimize the risk of vertical transmission of infection from parent to progeny, all IPNV infected broodstock populations had to undergo testing of all fish for the virus at the time of stripping and eggs from positive parents were destroyed. Between 1990 and 2002 over 68 000 Atlantic salmon broodfish were individually screened for IPNV by cell culture isolation and enzyme linked immunosorbent assay. Generalized linear ...
Source: Journal of Fish Diseases - November 20, 2009 Category: Zoology Authors: E S Munro, C P Millar, T S Hastings Source Type: journals

Establishment, characterization, virus susceptibility and transfection of cell lines from cobia, Rachycentron canadum (L.), brain and finEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Establishment and characterization of two cobia, Rachycentron canadum, cell lines derived from cobia brain (CB) and cobia fin (CF) are described. Caudal fin and brain from juvenile cobia were dissociated for 30 and 10 min, respectively, in phosphate-buffered saline containing 0.25% trypsin at 25 °C. The optimal culture condition for both dissociated cells (primary cell culture) was at 28 °C in Leibovitz-15 medium containing 10% foetal bovine serum. The cells have been sub-cultured at a ratio of 1:2 for more than 160 passages over a period of 3 years. Origin of the cultured cells was verified by comparison of their sequen...
Source: Journal of Fish Diseases - November 18, 2009 Category: Zoology Authors: T-C Cheng, Y-S Lai, I-Y Lin, C-P Wu, S-L Chang, T-I Chen, M-S Su Source Type: journals

A comparative molecular study of the presence of "Candidatus arthromitus" in the digestive system of rainbow trout, Oncorhynchus mykiss (Walbaum), healthy and affected with rainbow trout gastroenteritisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Observations were made using histopathological techniques in conjunction with a nested polymerase chain reaction (PCR) protocol for the specific detection of "Candidatus arthromitus" on DNA extracted from wax-embedded tissues and fresh digestive contents of rainbow trout. Samples positive for "Candidatus arthromitus" DNA included fish with rainbow trout gastroenteritis (RTGE), clinically normal cohabiting fish, and apparently healthy controls from RTGE positive and RTGE negative sites. The results obtained from the PCR were confirmed by nucleotide sequencing. "Candidatus arthromitus" DNA was found in distal intestine as we...
Source: Journal of Fish Diseases - November 13, 2009 Category: Zoology Authors: J Del-Pozo, J Turnbull, H Ferguson, M Crumlish Source Type: journals

Cellular components of probiotics control Yersinia ruckeri infection in rainbow trout, Oncorhynchus mykiss (Walbaum)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Subcellular components of the probiotics Aeromonas sobria GC2 and Bacillus subtilis JB-1, when administered to rainbow trout, Oncorhynchus mykiss, conferred protection against a new biogroup of Yersinia ruckeri. Thus, intraperitoneal or intramuscular injection of rainbow trout with cell wall proteins (CWPs), outer membrane proteins (OMPs), lipopolysaccharides (LPS), whole cell proteins (WCPs) and live cells followed by challenge on day 8 with Y. ruckeri led to 80[ndash]100% survival compared with 10% survival in the controls. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of WCPs and OMPs fr...
Source: Journal of Fish Diseases - November 12, 2009 Category: Zoology Authors: A Abbass, S M Sharifuzzaman, B Austin Source Type: journals

Utilization of tissue habitats by Myxobolus wulii Landsberg & Lom, 1991 in different carp hosts and disease resistance in allogynogenetic gibel carp: redescription of M. wulii from China and JapanEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Myxobolus wulii (=Myxosoma magna) was first described from the gills of goldfish, Carassius auratus auratus, in China. Subsequently, a myxosporean infecting the hepatopancreas of allogynogenetic gibel carp, C. auratus gibelio, was designated as a different species, Myxobolus guanqiaoensis, although the morphological features were almost identical to those of M. wulii. In Japan, an unidentified Myxobolus sp. was found in the gills and hepatopancreas of goldfish. Morphological and molecular analyses in the present study identified these myxosporeans as M. wulii, which was thus shown to use different habitats in the host fish...
Source: Journal of Fish Diseases - November 12, 2009 Category: Zoology Authors: J Y Zhang, H Yokoyama, J G Wang, A H Li, X N Gong, A Ryu-Hasegawa, M Iwashita, K Ogawa Source Type: journals

Host response to the chondracanthid copepod Chondracanthus goldsmidi, a gill parasite of the striped trumpeter, Latris lineata (Forster), in TasmaniaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study C. goldsmidi was associated with extensive epithelial hyperplasia and necrosis. Pathological changes were most pronounced near the parasite's attachment site, with papilloma-like growths surrounding the entire parasite resulting in deformation of the filament. The number of mucous cells increased near the parasite attachment sites on both the opercula and gills. Mast cells were absent in healthy gills; in contrast numerous mast cells were identified in the papilloma-like growths. Immunostaining identified piscidin-positive mast cells in the papilloma-like growths, presenting the first evidence of piscidin in ...
Source: Journal of Fish Diseases - November 12, 2009 Category: Zoology Authors: M Andrews, S Battaglene, J Cobcroft, M Adams, E Noga, B Nowak Source Type: journals

A sensitive FRET probe assay for the selective detection of Mycobacterium marinum in fishEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mycobacterium marinum is the causative agent of mycobacteriosis in wild and cultured fish and of atypical infection in humans. For the diagnosis of M. marinum, cultural and traditional polymerase chain reaction (PCR) methods are currently used. However, these protocols, although able to discriminate within Mycobacterium spp., have proved to be time-consuming or difficult to carry out. For this reason, the aim of this study was to obtain a rapid and specific diagnostic tool to quantify fish Mycobacterium spp. or to discriminate M. marinum from other mycobacteria. A primary PCR amplification with SYBR Green had a detection l...
Source: Journal of Fish Diseases - November 12, 2009 Category: Zoology Authors: F Salati, M Meloni, A Fenza, G Angelucci, A Colorni, G Orrù Source Type: journals

Attenuated Mycobacterium marinum protects zebrafish against mycobacteriosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - November 12, 2009 Category: Zoology Authors: Z Cui, D Samuel-Shaker, V Watral, M L Kent Source Type: journals

Complete sequencing of Tunisian redspotted grouper nervous necrosis virus betanodavirus capsid gene and RNA-dependent RNA polymerase geneEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study reports on the characterization of the full RNA2 sequence of a Tunisian betanodavirus with a length of 1433 nt, containing a 339 amino acid open-reading frame encoding the CP, and typing to the redspotted grouper nervous necrosis virus Ia genotype following phylogenetic analysis. The homology of the capsid protein to other betanodaviruses or alphanodaviruses was compared. In addition, a full length RNA1 sequence of 3104 nt encoding a 982 amino acid RNA-dependent RNA polymerase was obtained. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - November 12, 2009 Category: Zoology Authors: N Chérif, N Gagné, D Groman, F Kibenge, T Iwamoto, C Yason, S Hammami Source Type: journals

Mycobacteria isolated from Chesapeake Bay fishEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mycobacteriosis in fish can result in ulcers, emaciation, and in some cases death. Mycobacteria have been previously isolated from a variety of Chesapeake Bay fish species, and the current study was designed to identify potential host specificity and location fidelity of mycobacterial isolates. Mycobacteria were isolated from wild fish of the Chesapeake Bay collected from the Upper Bay, the Choptank River, Herring Bay, the Chicamacomico River, the Pocomoke River and the Potomac River in 2003[ndash]2006. Mycobacterial isolates were recovered from striped bass, Morone saxatilis, Atlantic menhaden, Brevoortia tyrannus, white ...
Source: Journal of Fish Diseases - November 10, 2009 Category: Zoology Authors: C B Stine, A S Kane, A M Baya Source Type: journals

Outbreaks of viral nervous necrosis in juvenile and adult farmed Atlantic cod, Gadus morhua L., in NorwayEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - November 5, 2009 Category: Zoology Authors: H Hellberg, A Kvellestad, B Dannevig, G Bornø, I Modahl, R N Haldorsen, F Vik-Mo, K Ottesen, E M Sætre, H Sindre Source Type: journals

Myxobolus notropis from emerald shiner, Notropis atherinoides Rafinesque, in Lake SuperiorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - November 2, 2009 Category: Zoology Authors: D Horner, S D Atkinson, D M Pratt, S Marcquenski, J L Bartholomew Source Type: journals

Koi herpesvirus: distribution and prospects for control in England and WalesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to determine the geographic distribution and prevalence of KHV exposed fish in England and Wales through ELISA antibody testing. Only three of the 82 farms sampled produced positive results, suggesting fish farms provide a relatively safe source of fish. Of the 71 'high-risk' fisheries tested, 26 were positive. All eight geographic areas within England and Wales studied had at least one KHV positive site. Twelve consignments of imported koi carp from seven S.E. Asian countries were tested for KHV antibody. Six consignments from six different countries were positive. Although a high proportion of consignmen...
Source: Journal of Fish Diseases - October 30, 2009 Category: Zoology Authors: N G H Taylor, P F Dixon, K R Jeffery, E J Peeler, K L Denham, K Way Source Type: journals

Tunic morphology and viral surveillance in diseased Korean ascidians: soft tunic syndrome in the edible ascidian, Halocynthia roretzi (Drasche), in aquacultureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
'Soft tunic syndrome' causes mass mortality in the edible ascidian Halocynthia roretzi in Korean and Japanese aquaculture. In histopathological comparison, there were no specific differences between diseased specimens from Korea and Japan, indicating that soft tunic syndrome occurring in Korea and Japan is the same disease. No bacterial or protozoan cells were microscopically detected in either healthy or diseased tunics suggesting they are not the direct causes of soft tunic syndrome. Attempts were made to isolate virus from affected ascidians taking into account temperature conditions in which soft tunic syndrome is most...
Source: Journal of Fish Diseases - October 30, 2009 Category: Zoology Authors: S-I Kitamura, S-I Ohtake, J-Y Song, S-J Jung, M-J Oh, B-D Choi, K Azumi, E Hirose Source Type: journals

First isolation of Tenacibaculum soleae from diseased cultured wedge sole, Dicologoglossa cuneata (Moreau), and brill, Scophthalmus rhombus (L.)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - October 30, 2009 Category: Zoology Authors: J R López, M Piñeiro-Vidal, N García-Lamas, R de la Herran, J I Navas, I Hachero-Cruzado, Y Santos Source Type: journals

In vitro markers for virulence in Yersinia ruckeriEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, different traits that have been associated with bacterial virulence were studied in Yersinia ruckeri. Two isolates that had been shown to cause disease and mortality in experimentally infected rainbow trout were compared with five avirulent isolates. Both virulent isolates showed high adhesion to gill and intestinal mucus of rainbow trout, whereas the majority of non-virulent strains demonstrated significantly lower adhesion. A decrease in adherence capability following bacterial treatment with sodium metaperiodate and proteolytic enzymes suggested the involvement of carbohydrates and proteins. All strains w...
Source: Journal of Fish Diseases - October 30, 2009 Category: Zoology Authors: E Tobback, A Decostere, K Hermans, W Van den Broeck, F Haesebrouck, K Chiers Source Type: journals

The effect of liposome-coated recombinant protein VP28 against white spot syndrome virus in kuruma shrimp, Marsupenaeus japonicusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - September 3, 2009 Category: Zoology Authors: R Mavichak, T Takano, H Kondo, I Hirono, S Wada, K Hatai, H Inagawa, Y Takahashi, T Yoshimura, H Kiyono, Y Yuki, T Aoki Source Type: journals

Phenotypic, serological and molecular evidence of Chryseobacterium piscicola in farmed Atlantic salmon, Salmo salar L., in FinlandEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - September 3, 2009 Category: Zoology Authors: P Ilardi, J Abad, P Rintamäki, J-F Bernardet, R Avendaño-Herrera Source Type: journals

Exophiala xenobiotica infection in cultured striped jack, Pseudocaranx dentex (Bloch & Schneider), in JapanEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This report describes Exophiala infection in cultured striped jack, Pseudocaranx dentex, in Japan in 2005. One hundred out of 35 000 fish died per day and mortalities continued for 1 month. Diseased fish showed swelling of the abdomen and kidney distension. Numerous septate hyphae, pale brown in colour, were seen in kidney in squash preparations. Histology revealed abundant fungal hyphae and conidia in gill, heart and kidney. Fungal hyphae were accompanied by cell necrosis and influx of inflammatory, mainly mononuclear cells. The fungus isolated from the diseased fish had septate hyphae, pale brown in colour and 1.8[ndash]...
Source: Journal of Fish Diseases - September 3, 2009 Category: Zoology Authors: C Munchan, O Kurata, S Wada, K Hatai, A Sano, K Kamei, N Nakaoka Source Type: journals

Pharmacokinetics of emamectin benzoate administered to Atlantic salmon, Salmo salar L., by intra-peritoneal injectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - August 30, 2009 Category: Zoology Authors: K A Glover, O B Samuelsen, O T Skilbrei, K Boxaspen, B T Lunestad Source Type: journals

Experimental studies confirm the wide host range of the Great Lakes viral haemorrhagic septicaemia virus genotype IVbEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - August 29, 2009 Category: Zoology Authors: R Kim, M Faisal Source Type: journals

Book reviewEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - August 29, 2009 Category: Zoology Authors: R.J. Roberts Source Type: journals

Prospective longitudinal studies of salmonid alphavirus infections on two Atlantic salmon farms in Ireland; evidence for viral persistenceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Prospective longitudinal studies of two outbreaks of pancreas disease in Atlantic salmon (AS), Salmo salar L., in Ireland were conducted. Both outbreaks occurred during the marine phase of production, with one caused by salmonid alphavirus subtype 1 (SAV1) and the other by SAV4. In addition to screening a range of tissues by real-time reverse transcriptase polymerase chain reaction (RRT-PCR), virological, serological and histopathological examinations were performed along with partial genome sequencing and results were related to environmental and production data and farm history. On Farm 1 (marine sampling only), infectio...
Source: Journal of Fish Diseases - August 29, 2009 Category: Zoology Authors: D A Graham, E Fringuelli, C Wilson, H M Rowley, A Brown, H Rodger, M F McLoughlin, C McManus, E Casey, L J McCarthy, N M Ruane Source Type: journals

A single-dose pharmacokinetic study of emamectin benzoate in cod, Gadus morhua L., held in sea water at 9 °CEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The pharmacokinetic profile of the antiparasitic agent emamectin benzoate was studied in plasma after intravenous (i.v.) injection and in plasma, muscle and skin following oral (p.o.) administration to cod, Gadus morhua, held in sea water at 9 °C and weighing 100[ndash]200 g. Following i.v. injection, the plasma drug concentration-time profile showed two distinct phases. The plasma distribution half-life (t1/2[alpha]) was estimated as 2.5 h, the elimination half-life (t1/2[beta]) as 216 h, the total body clearance (ClT) as 0.0059 L kg[minus]1 h[minus]1 and mean residence time (MRT) as 385 h. The volume of distribution at ...
Source: Journal of Fish Diseases - August 29, 2009 Category: Zoology Authors: O B Samuelsen Source Type: journals

An experimental vaccine against Aeromonas hydrophila can induce protection in rainbow trout, Oncorhynchus mykiss (Walbaum)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A candidate vaccine against Aeromonas hydrophila in rainbow trout, Oncorhynchus mykiss, was developed using a bacterial lysate. To test the strength of protection, A. hydrophila challenge models were compared using injection into both the intraperitoneal (IP) cavity and the dorsal sinus (DS) with selected doses of live bacteria washed in saline or left untreated. Unlike the IP route, injection into the DS with either saline washed or unwashed cells resulted in consistent cumulative mortality and a dose response that could be used to establish a standard challenge having an LD50 of approximately 3 × 107 colony forming unit...
Source: Journal of Fish Diseases - August 29, 2009 Category: Zoology Authors: S E LaPatra, K P Plant, S Alcorn, V Ostland, J Winton Source Type: journals

Intensive infestations of Sciaenocotyle pancerii (Monogenea, Microcotylidae) on Argyrosomus regius (Asso) under fish-farming conditionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - August 20, 2009 Category: Zoology Authors: S Ternengo, S Agostini, Y Quilichini, L Euzet, B Marchand Source Type: journals

Intramyocardial dissecting haemorrhage in farmed rainbow trout Oncorhynchus mykiss (Walbaum)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - August 19, 2009 Category: Zoology Authors: T Poppe, B Tørud Source Type: journals

Challenge studies of European stocks of redfin perch, Perca fluviatilis L., and rainbow trout, Oncorhynchus mykiss (Walbaum), with epizootic haematopoietic necrosis virusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A challenge model for comparison of the virulence of epizootic haematopoietic necrosis virus (EHNV) to European stocks of redfin perch, Perca fluviatilis L., and rainbow trout, Oncorhynchus mykiss (Walbaum), was tested. The model investigated intraperitoneal (IP), bath and cohabitation routes at 10, 15 and 20 °C for 5[ndash]6 g fish and 15 °C for 20 g perch. In the IP challenges of perch, significant mortality occurred at 15 °C and 20 °C. In challenge trials for rainbow trout, significant mortalities were observed in IP and bath challenges at 20 °C. The mortality observed in IP challenged 20 g perch was not significan...
Source: Journal of Fish Diseases - August 19, 2009 Category: Zoology Authors: E Ariel, B Bang Jensen Source Type: journals

Antigenic differences of the scuticociliate Miamiensis avidus from JapanEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we elucidated the antigenic differences between six Japanese M. avidus isolates as an initial step toward developing an effective vaccine against the disease. Four Japanese flounder isolates (IyoI, Nakajima, JF05To and Mie0301 isolates), one spotted knifejaw, Oplegnathus punctatus, isolate (SK05Kyo), and one ridged-eye flounder, Pleuronichthys cornutus, isolate (RF05To) were subjected to serological analysis. Antisera against IyoI, SK05Kyo, Nakajima and Mie0301 isolates were raised in rabbits and used for immobilization assays and Western blotting. Immobilization assays showed that the six isolates could be ...
Source: Journal of Fish Diseases - August 19, 2009 Category: Zoology Authors: J-Y Song, K Sasaki, T Okada, M Sakashita, H Kawakami, S Matsuoka, H-S Kang, K Nakayama, S-J Jung, M-J Oh, S-I Kitamura Source Type: journals

In vitro efficacy of praziquantel against the cercariae of Diplostomum sp., Rhipidocotyle fennica and R. campanulaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - July 13, 2009 Category: Zoology Authors: A Voutilainen, M Saarinen, A Suonpää, J Taskinen Source Type: journals

Development and characterization of a monoclonal antibody against Taura syndrome virusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We produced a panel of monoclonal antibodies (MAbs) from the fusion of Taura syndrome virus variants from Belize (TSV-BZ) immunized BALB/cJ mouse spleen cells and non-immunoglobulin secreting SP2/0 mouse myeloma cells. One antibody, 2C4, showed strong specificity and sensitivity for TSV in dot-blot immunoassay and immunohistochemistry (IHC) analysis. The MAb reacted against native TSV-BZ, TSV variants from Sinaloa, Mexico (TSV-SI) and TSV variants from Hawaii (TSV-HI) in dot-blot immunoassay. By IHC, the antibody identified the virus in a pattern similar to the digoxigenin-labelled TSV-cDNA probe for the TSV-BZ, TSV-HI and...
Source: Journal of Fish Diseases - July 12, 2009 Category: Zoology Authors: I Côté, B T Poulos, R M Redman, D V Lightner Source Type: journals

Identification and pathogenicity to rainbow trout, Oncorhynchus mykiss (Walbaum), of some aeromonadsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Twelve strains of fish pathogenic aeromonads were identified by 16S rRNA sequencing as Aeromonas bestiarum, A. hydrophila, A. hydrophila subsp. dhakensis, A. salmonicida subsp. salmonicida, A. sobria biovar sobria and A. veronii biovar sobria. Following intramuscular injection, A. hydrophila subsp. dhakensis caused dark liquefying, raised furuncle-like lesions in rainbow trout within 48 h. Extracellular products of all cultures contained gelatinase and lecithinase, and most revealed lipase. Congo red absorption and siderophore production was recorded, but not so the suicide phenomenon or slime production. Sodium dodecyl su...
Source: Journal of Fish Diseases - July 9, 2009 Category: Zoology Authors: P Orozova, M Barker, D A Austin, B Austin Source Type: journals

Melanomacrophages in three species of free-ranging sharks from the northwestern Atlantic, the blue shark Prionacae glauca (L.), the shortfin mako, Isurus oxyrhinchus Rafinesque, and the thresher, Alopias vulpinus (Bonnaterre)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The melanomacrophage aggregates or cells (MMC) are commonly used as biomarkers of exposure to pollution in fish, albeit their numbers and morphological characteristics can be influenced not only by environmental toxins but also by a range of physiological parameters and pathological conditions. Accordingly, before we can use MMC as biomarkers in any fish species, their normal, 'background' characteristics have to be established in apparently healthy fish. The knowledge of MMC in sharks is minimal. The aim of this study was to characterize MMC from 51 free-ranging, large pelagic sharks from the northwestern Atlantic, includ...
Source: Journal of Fish Diseases - July 9, 2009 Category: Zoology Authors: J D Borucinska, K Kotran, M Shackett, T Barker Source Type: journals

Increased survival by feeding tetradecylthioacetic acid during a natural outbreak of heart and skeletal muscle inflammation in S0 Atlantic salmon, Salmo salar L.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We have previously documented increased survival by feeding tetradecylthioacetic acid (TTA) during a natural outbreak of infectious pancreatic necrosis in post-smolt S1 Atlantic salmon. The aim of the present study was to test the effects of dietary TTA in S0 smolt at a location where fish often experience natural outbreaks of heart and skeletal muscle inflammation (HSMI) during their first spring at sea. The experimental groups were fed a diet supplemented with 0.25% TTA for a 6-week period prior to a natural outbreak of HSMI in May 2007. Relative percent survival for the groups fed TTA was 45% compared with control diets...
Source: Journal of Fish Diseases - July 9, 2009 Category: Zoology Authors: H Alne, M S Thomassen, H Takle, B F Terjesen, F Grammes, M Oehme, S Refstie, T Sigholt, R K Berge, K-A Rørvik Source Type: journals

Molecular differentiation of infectious pancreatic necrosis virus isolates from farmed and wild salmonids in IrelandEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigated the genotypes and sub-groups of infectious pancreatic necrosis virus (IPNV) present in farmed and wild salmonid fish in Ireland. An 1100-bp portion of the VP2 region of segment A from each of 55 IPNV isolates collected over 2003[ndash]2007 was amplified by reverse-transcription[ndash]polymerase chain reaction and the product directly sequenced. The nucleotide sequences of each isolate were aligned and compared with each other and with the corresponding sequences of a number of reference isolates. All the 55 sequenced isolates belonged to genogroup 5 (Sp serotype) and could be divided into two subgro...
Source: Journal of Fish Diseases - July 9, 2009 Category: Zoology Authors: N M Ruane, L J McCarthy, D Swords, K Henshilwood Source Type: journals

Comparison of the resistance of selected families of common carp, Cyprinus carpio L., to koi herpesvirus: preliminary studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - July 6, 2009 Category: Zoology Authors: P F Dixon, C L Joiner, K Way, R A Reese, G Jeney, Z Jeney Source Type: journals

Caspase-dependent induction of apoptosis in barramundi, Lates calcarifer (Bloch), muscle cells by grouper iridovirusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We recently reported that grouper iridovirus (GIV) can induce apoptosis in barramundi, Lates calcarifer, muscle (BM) and swim bladder (BSB) cell lines. In this paper, we further characterize the molecular mechanism underlying apoptotic death in BM cells triggered by GIV. DNA-laddering and apoptotic cells were observed in BM cells infected with UV-irradiated or untreated GIV but was absent in cells infected with heat-inactivated GIV, indicating the involvement of viral protein in the apoptosis event. In GIV-infected BM cells, the conversion of procaspase-3 to caspase-3 was evident and the level of caspase-8 and -9 increased...
Source: Journal of Fish Diseases - July 6, 2009 Category: Zoology Authors: P P Chiou, Y-C Chen, Y-S Lai Source Type: journals

Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A bacterium was isolated from the mid-gut of healthy black tiger shrimp, Penaeus monodon, based on a large zone of inhibition in mixed culture on solid medium. The isolate was a Gram-positive, motile spore former, with an optimum pH range for growth in tryptone soya broth containing 2% NaCl of between pH 6 and 9. The bacterium was highly salt tolerant with concentrations between 0% and 8% having no detrimental effect on growth. The isolate was identified as Bacillus pumilus based on physiological capabilities using the API50CHB and Biolog systems. Amplification and sequencing of the 16S rRNA gene followed by phylogenetic a...
Source: Journal of Fish Diseases - July 4, 2009 Category: Zoology Authors: J E Hill, J C F Baiano, A C Barnes Source Type: journals

Effects of temperature on disease progression and swimming stamina in Ichthyophonus-infected rainbow trout, Oncorhynchus mykiss (Walbaum)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study helps explain field observations showing the recent emergence of clinical ichthyophoniasis in Yukon River Chinook salmon later in their spawning migration when water temperatures were high, as well as the apparent failure of a substantial percentage of infected fish to successfully reach their natal spawning areas. (Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - June 30, 2009 Category: Zoology Authors: R Kocan, P Hershberger, G Sanders, J Winton Source Type: journals

FishPathogens.eu/vhsv: a user-friendly viral haemorrhagic septicaemia virus isolate and sequence databaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A database has been created, http://www.FishPathogens.eu, with the aim of providing a single repository for collating important information on significant pathogens of aquaculture, relevant to their control and management. This database will be developed, maintained and managed as part of the European Community Reference Laboratory for Fish Diseases function. This concept has been initially developed for viral haemorrhagic septicaemia virus and will be extended in future to include information on other significant aquaculture pathogens. Information included for each isolate comprises sequence, geographical origin, host ori...
Source: Journal of Fish Diseases - June 24, 2009 Category: Zoology Authors: S P Jonstrup, T Gray, S Kahns, H F Skall, M Snow, N J Olesen Source Type: journals

Genetic heterogeneity of betanodaviruses in juvenile production trials of Pacific bluefin tuna, Thunnus orientalis (Temminck & Schlegel)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we examined the genetic variability of betanodaviruses detected in the diseased juveniles to understand the transmission of the disease in a tuna hatchery. A total of 94 nucleotide sequences of betanodavirus (partial sequence of the coat protein gene, RNA2) were obtained from fish samples by reverse-transcriptase polymerase chain reaction amplification and 13 haplotypes were recognized among the sequences. The haplotype distributions in the viral populations from the diseased juveniles were related to the broodstocks from which the juveniles originated, suggesting that vertical transmission had occurred in t...
Source: Journal of Fish Diseases - June 16, 2009 Category: Zoology Authors: T Sugaya, K Mori, T Nishioka, S Masuma, M Oka, K Mushiake, Y Okinaka, T Nakai Source Type: journals

Development of a method for the detection of infectious myonecrosis virus by reverse-transcription loop-mediated isothermal amplification and nucleic acid lateral flow hybrid assayEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report the development of a reverse-transcription loop-mediated isothermal amplification and nucleic acid lateral flow method (RT-LAMP-NALF) for detection of infectious myonecrosis virus (IMNV). The RT-LAMP-NALF method combines simplified nucleic acid extraction, a reverse-transcription loop-mediated isothermal amplification platform, and one-step visual colorimetric confirmation of the IMNV amplified sequences using a generic NALF qualitative detection test strip. The sensitivity of RT-LAMP (using two and three primer pairs) and nested RT-LAMP (using three primer pairs) was compared by real-time reverse-transcription-p...
Source: Journal of Fish Diseases - June 15, 2009 Category: Zoology Authors: T P D Andrade, D V Lightner Source Type: journals

Husbandry stress exacerbates mycobacterial infections in adult zebrafish, Danio rerio (Hamilton)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mycobacteria are significant pathogens of laboratory zebrafish, Danio rerio (Hamilton). Stress is often implicated in clinical disease and morbidity associated with mycobacterial infections but has yet to be examined with zebrafish. The aim of this study was to examine the effects of husbandry stressors on zebrafish infected with mycobacteria. Adult zebrafish were exposed to Mycobacterium marinum or Mycobacterium chelonae, two species that have been associated with disease in zebrafish. Infected fish and controls were then subjected to chronic crowding and handling stressors and examined over an 8-week period. Whole-body c...
Source: Journal of Fish Diseases - June 15, 2009 Category: Zoology Authors: J M Ramsay, V Watral, C B Schreck, M L Kent Source Type: journals

First isolation and characterization of Lactococcus garvieae from Brazilian Nile tilapia, Oreochromis niloticus (L.), and pintado, Pseudoplathystoma corruscans (Spix & Agassiz)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lactococcus garvieae infection in cultured Nile tilapia, Oreochromis niloticus (L.), and pintado, Pseudoplathystoma corruscans (Spix & Agassiz), from Brazil is reported. The commercial bacterial identification system, Biolog Microlog®, confirmed the identity of L. garvieae. Infectivity trials conducted in Nile tilapia using Brazilian Nile tilapia L. garvieae isolates resulted in a median lethal dose-50 of 1.4 × 105 colony-forming units (CFU)/fish. This is the first evidence of the presence of this pathogen from Brazilian fish. In addition, this is the first report of L. garvieae infection in either Nile tilapia or pintad...
Source: Journal of Fish Diseases - June 15, 2009 Category: Zoology Authors: J J Evans, P H Klesius, C A Shoemaker Source Type: journals

Effect of extracellular products of Tenacibaculum maritimum in Atlantic salmon, Salmo salar L.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - June 14, 2009 Category: Zoology Authors: R van Gelderen, J Carson, B Nowak Source Type: journals

Fitness and virulence of different strains of white spot syndrome virusEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - June 14, 2009 Category: Zoology Authors: B. Pradeep, I. Karunasagar, I. Karunasagar Source Type: journals

Comparison of histological features, and description of histopathological lesions in thyroid glands from three species of free-ranging sharks from the northwestern Atlantic, the blue shark, Prionace glauca (L.), the shortfin mako, Isurus oxyrhinchus Rafinesque, and the thresher, Alopias vulpinus (Bonnaterre)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Histomorphological features of piscine thyroids are widely accepted and frequently used as bioindicators of environmental pollution. This despite the fact that there is marked variation in thyroid morphology resulting from numerous pathological and physiological conditions. Our hypothesis was that there will be variations in histological features in thyroids collected from different shark species during the summer season in the northwestern Atlantic. To test our hypothesis, we examined histological features encountered in grossly normal thyroids from three species of sharks, the shortfin mako, Isurus oxyrhinchus, thresher,...
Source: Journal of Fish Diseases - June 14, 2009 Category: Zoology Authors: J D Borucinska, M Tafur Source Type: journals

Idiopathic cardiac pathology in seawater-farmed rainbow trout, Oncorhynchus mykiss (Walbaum)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Fish Diseases)
Source: Journal of Fish Diseases - June 14, 2009 Category: Zoology Authors: T T Poppe, G Bornø, L Iversen, E Myklebust Source Type: journals

Lateral line depigmentation (LLD) in channel catfish, Ictalurus punctatus (Rafinesque)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present microbiological, immunological and histopathological features of this lesion in channel catfish, Ictalurus punctatus (Rafinesque), that developed after exposure to a chronic nutritional stress. Depigmention was limited to skin that was adjacent to the lateral line. The epidermis of affected fish was thin and reduced to a one-cell-thick layer over the lateral line. Melanocytes were depleted at the dermo-epidermal junction and formed aggregates in the epidermis. Innate immunity was weaker in affected fish than that previously measured in well-fed channel catfish. Because the pathology and apparent aetiology of HLL...
Source: Journal of Fish Diseases - June 14, 2009 Category: Zoology Authors: J Corrales, A Ullal, E J Noga Source Type: journals

Francisella sp., an emerging pathogen of tilapia, Oreochromis niloticus (L.), in Costa RicaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Francisella sp. is an emergent bacterial pathogen that causes acute to chronic disease in warm and cold water cultured and wild fish species. During the past 3 years, the bacterium has been detected in tilapia, Oreochromis niloticus, cultured in Costa Rica. Infected fish presented non-specific clinical signs, such as erratic swimming, anorexia, anaemia, exophthalmia and high mortality. Upon macroscopic and microscopic examination, several internal organs (mainly spleen and kidney) were enlarged and contained white nodules. Histological examination revealed the presence of multifocal granulomatous lesions, with the presence...
Source: Journal of Fish Diseases - June 8, 2009 Category: Zoology Authors: E Soto, J P Hawke, D Fernandez, J A Morales Source Type: journals