Journal of Histochemistry and Cytochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Variability in Platelet-Derived Growth Factor Receptor Alpha Antibody Specificity May Impact Clinical Utility of Immunohistochemistry Assays
Aberrant regulation of the receptor tyrosine kinase platelet-derived growth factor alpha (PDGFRα) is implicated in several types of cancer. Inhibition of the PDGFRα pathway may be a beneficial therapy, and detection of PDGFRα in tumor biopsies may lead to insights about which patients respond to therapy. Exploratory or clinical biomarker use of PDGFRα IHC has been frequently reported, often with polyclonal antibody sc-338. An sc-338-based assay was systematically compared with anti-PDGFRα rabbit monoclonal antibody D13C6 using immunoblot profiling and IHC in formalin-fixed and paraffin-embedde...
Source: Journal of Histochemistry and Cytochemistry - November 28, 2016 Category: Biochemistry Authors: Holzer, T. R., ONeill Reising, L., Credille, K. M., Schade, A. E., Oakley, G. J. Tags: Articles Source Type: research

Megalin Is Predominantly Observed in Vesicular Structures in First and Third Trimester Cytotrophoblasts of the Human Placenta
The membrane receptor megalin is crucial for normal fetal development. Besides its expression in the developing fetus, megalin is also expressed in the human placenta. Similar to its established function in the kidney proximal tubules, placental megalin has been proposed to mediate uptake of vital nutrients. However, details of megalin expression, subcellular localization, and function in the human placenta remain to be established. By immunohistochemical analyses of first trimester and term human placenta, we showed that megalin is predominantly expressed in cytotrophoblasts, the highly proliferative cells in placenta. On...
Source: Journal of Histochemistry and Cytochemistry - November 28, 2016 Category: Biochemistry Authors: Storm, T., Christensen, E. I., Christensen, J. N., Kjaergaard, T., Uldbjerg, N., Larsen, A., Honore, B., Madsen, M. Tags: Articles Source Type: research

Altered T-Cell Balance in Lymphoid Organs of a Mouse Model of Colorectal Cancer
The adenomatous polyposis coli (APC) gene is a known tumor suppressor gene, and mice with mutations in Apc (ApcMin/+) spontaneously form multiple intestinal neoplasms. In this model of human colorectal cancer (CRC), it has been reported that CD4+ T-cell-derived interleukin 17 (IL-17) promotes intestinal tumor development, but it is not known if the Apc mutation actually directly alters T-cell function and subsequently tumor immunosurveillance. To investigate the ApcMin/+ mutation on T-cell function, flow cytometric, histochemical, and immunofluorescent studies on both wild-type (Apc+/+) and ApcMin/+ mice were performed. We...
Source: Journal of Histochemistry and Cytochemistry - November 28, 2016 Category: Biochemistry Authors: Tanner, S. M., Daft, J. G., Hill, S. A., Martin, C. A., Lorenz, R. G. Tags: Articles Source Type: research

Cell Line Macroarray: An Alternative High-Throughput Platform to Analyze hiPSC Lines
In the past decade, tissue microarray (TMA) technology has evolved as an innovative tool for high-throughput proteomics analysis and mainly for biomarker validation. Similarly, enormous amount of data can be obtained from the cell line macroarray (CLMA) technology, which developed from the TMA using formalin-fixed, paraffin-embedded cell pellets. Here, we applied CLMA technology in stem cell research and in particular to identify bona fide neogenerated human induced pluripotent stem cell (hiPSC) clones suitable for down the line differentiation. All hiPSC protocols generate tens of clones, which need to be tested to determ...
Source: Journal of Histochemistry and Cytochemistry - November 28, 2016 Category: Biochemistry Authors: La Spada, A., Baronchelli, S., Ottoboni, L., Ruffini, F., Martino, G., Convertino, N., Ntai, A., Steiner, T., Biunno, I., De Blasio, A. Tags: Articles Source Type: research

Immunophenotyping of Rabbit Testicular Germ and Sertoli Cells Across Maturational Stages
During testicular maturation, both Sertoli cells (SCs) and germ cells (GCs) switch from an immature to a mature immunophenotype. The reexpression of markers of immaturity in adults has been reported in cancer and in other testicular pathologies, in men as well as in animal species. Naturally affected with testicular cancer, rabbits have long been used in human reproductive research, but reports on the expression of testicular cell markers in this species are few and data about the immunophenotype of normal postnatal SCs and GCs are lacking. The aim of this study was to investigate the immunophenotype of SCs and GCs in the ...
Source: Journal of Histochemistry and Cytochemistry - October 24, 2016 Category: Biochemistry Authors: Banco, B., Grilli, G., Giudice, C., Marques, A. T., Cotti Cometti, S., Visigalli, G., Grieco, V. Tags: Articles Source Type: research

Differential Lectin Binding Patterns Identify Distinct Heart Regions in Giant Danio (Devario aequipinnatus) and Zebrafish (Danio rerio) Hearts
Lectins are carbohydrate-binding proteins commonly used as biochemical and histochemical tools to study glycoconjugate (glycoproteins, glycolipids) expression patterns in cells, tissues, including mammalian hearts. However, lectins have received little attention in zebrafish (Danio rerio) and giant danio (Devario aequipinnatus) heart studies. Here, we sought to determine the binding patterns of six commonly used lectins—wheat germ agglutinin (WGA), Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin (BS lectin), concanavalin A (Con A), Ricinus communis agglutinin I (RCA I), and Lycopersicon esculentum agglutin...
Source: Journal of Histochemistry and Cytochemistry - October 24, 2016 Category: Biochemistry Authors: Manalo, T., May, A., Quinn, J., Lafontant, D. S., Shifatu, O., He, W., Gonzalez-Rosa, J. M., Burns, G. C., Burns, C. E., Burns, A. R., Lafontant, P. J. Tags: Articles Source Type: research

Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction: DNA Repair and Epigenetics of Ribosomal Genes
DNA damage response (DDR) in ribosomal genes and mechanisms of DNA repair in embryonic stem cells (ESCs) are less explored nuclear events. DDR in ESCs should be unique due to their high proliferation rate, expression of pluripotency factors, and specific chromatin signature. Given short population doubling time and fast progress through G1 phase, ESCs require a sustained production of rRNA, which leads to the formation of large and prominent nucleoli. Although transcription of rRNA in the nucleolus is relatively well understood, little is known about DDR in this nuclear compartment. Here, we directed formation of double-st...
Source: Journal of Histochemistry and Cytochemistry - October 24, 2016 Category: Biochemistry Authors: Franek, M., Kovarikova, A., Bartova, E., Kozubek, S. Tags: Articles Source Type: research

Sialidase Unmasks Mucin Domain Epitopes of Lubricin
Lubricin is a secreted, mucin-like glycoprotein and proteoglycan abundant in synovial fluid that provides boundary lubrication and prevents cell adhesion in synovial joints. The antilubricin S6.79 monoclonal antibody recognizes an O-linked glycopeptide epitope in lubricin’s mucin domain. The central, long mucin domain of lubricin is extensively O-glycosylated with Gal(β1-3)GalNAc-O-Ser/Thr, and about two thirds of the O-glycosylated sites are capped with sialic acid. Our aim was to determine whether removal of sialic acid by sialidase could improve the detection of lubricin in a number of human tissues using the...
Source: Journal of Histochemistry and Cytochemistry - October 24, 2016 Category: Biochemistry Authors: Solka, K. A., Miller, I. J., Schmid, T. M. Tags: Articles Source Type: research

Matrix Metalloproteinase 20 Co-expression With Dentin Sialophosphoprotein in Human and Monkey Kidneys
This study investigated the expression of MMP20 and verified its co-expression with DSPP in human and monkey kidney sections and human mixed renal cells by IHC, in situ proximity ligation assay, and immunofluorescence. Our results show that MMP20 is expressed in all segments of the human and monkey nephron with marked intensity in the proximal and distal tubules, and was absent in the glomeruli. Furthermore, MMP20 co-expressed with DSPP in the proximal, distal, and collecting tubules, and in mixed renal cells. Consistent with other SIBLING–MMP pairs, the DSPP–MMP20 pair may play a role in the normal turnover of...
Source: Journal of Histochemistry and Cytochemistry - September 24, 2016 Category: Biochemistry Authors: Ogbureke, K. U. E., Koli, K., Saxena, G. Tags: Articles Source Type: research

Localization of Minodronate in Mouse Femora Through Isotope Microscopy
Minodronate is highlighted for its marked and sustained effects on osteoporotic bones. To determine the duration of minodronate’s effects, we have assessed the localization of the drug in mouse bones through isotope microscopy, after labeling it with a stable nitrogen isotope ([15N]-minodronate). In addition, minodronate-treated bones were assessed by histochemistry and transmission electron microscopy (TEM). Eight-week-old male ICR mice received [15N]-minodronate (1 mg/kg) intravenously and were sacrificed after 3 hr, 24 hr, 1 week, and 1 month. Isotope microscopy showed that [15N]-minodronate was present mainly ben...
Source: Journal of Histochemistry and Cytochemistry - September 24, 2016 Category: Biochemistry Authors: Hongo, H., Sasaki, M., Kobayashi, S., Hasegawa, T., Yamamoto, T., Tsuboi, K., Tsuchiya, E., Nagai, T., Khadiza, N., Abe, M., Kudo, A., Oda, K., Henrique Luiz de Freitas, P., Li, M., Yurimoto, H., Amizuka, N. Tags: Articles Source Type: research

Investigation of PD-L1 Biomarker Testing Methods for PD-1 Axis Inhibition in Non-squamous Non-small Cell Lung Cancer
This study compares multiple methods of biomarker testing for treatment of NSCLCs with PD1-axis inhibitors. Tissue microarrays of matched primary and metastatic NSCLCs were used to compare four different PD-1 ligand (PD-L1) IHC techniques, as well as RNA ISH. Additional cases with whole genome and transcriptome data were assessed for molecular correlates of PD-L1 overexpression. Eighty cases were included in the IHC study. Multiple IHC methodologies showed a high rate of agreement (Kappa = 0.67). When calibrated to RNA expression, agreement improved significantly (Kappa = 0.90, p=0.0049). PD-L1 status of primary and metast...
Source: Journal of Histochemistry and Cytochemistry - September 24, 2016 Category: Biochemistry Authors: Sheffield, B. S., Fulton, R., Kalloger, S. E., Milne, K., Geller, G., Jones, M., Jacquemont, C., Zachara, S., Zhao, E., Pleasance, E., Laskin, J., Jones, S. J. M., Marra, M. A., Yip, S., Nelson, B. H., Gown, A. M., Ho, C., Ionescu, D. N. Tags: Articles Source Type: research

Comments on Methods to Suppress Endogenous {beta}-Galactosidase Activity in Mouse Tissues Expressing the LacZ Reporter Gene
The Escherichia coli LacZ gene (encoding β-galactosidase) is a widely used reporter for gene regulation analysis in transgenic mice. Determination of β-galactosidase activity is classically performed using 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside/ferri-/ferrocyanide (X-Gal/FeCN) histochemistry. Uncertainty about the origin of the β-galactosidase signal is encountered in tissues containing high levels of endogenous β-galactosidase. Here, we show that reliable results can nevertheless be obtained in these tissues by performing the histochemical reaction under slightly basic pH conditions (pH ...
Source: Journal of Histochemistry and Cytochemistry - September 24, 2016 Category: Biochemistry Authors: Merkwitz, C., Blaschuk, O., Schulz, A., Ricken, A. M. Tags: Articles Source Type: research

Clinical Significance of TIPE2 Protein Upregulation in Non-Hodgkins Lymphoma
In this study, we determined TIPE2 expression in NHL by immunohistochemistry and investigated its clinicopathological significance in DLBCL. We found that TIPE2 expression was upregulated in both DLBCL and peripheral T-cell lymphoma. But the expression of TIPE2 in T lymphomas was weaker than that in DLBCL. Interestingly, among DLBCL, TIPE2 expression was significantly stronger in the germinal center B-cell (GCB) type than in the non-GCB type. These results suggest that the expression of TIPE2 protein could be a predictor of better prognosis for DLBCL. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - August 29, 2016 Category: Biochemistry Authors: Hao, C., Zhang, N., Geng, M., Ren, Q., Li, Y., Wang, Y., Chen, Y. H., Liu, S. Tags: Articles Source Type: research

The Use of Cytochrome C Oxidase Enzyme Activity and Immunohistochemistry in Defining Mitochondrial Injury in Kidney Disease
The renal biopsy is a dynamic way of looking at renal disease, and tubular elements are an important part of this analysis. The mitochondria in 20 renal biopsies were examined by immunohistochemical (electron transport chain enzyme: cytochrome C oxidase IV [COX IV]) and enzyme histochemical methods (COX), both by light and electron microscopy. The distal convoluted tubules and thick ascending limbs showed the greatest intensity in the COX immunostains and enzyme activity in controls. The degree of mitochondrial COX protein and enzyme activity diminished as the tubules became atrophic. With proximal hypertrophic changes, th...
Source: Journal of Histochemistry and Cytochemistry - August 29, 2016 Category: Biochemistry Authors: Zsengeller, Z. K., Rosen, S. Tags: Articles Source Type: research

Effects of Oxaliplatin Treatment on the Enteric Glial Cells and Neurons in the Mouse Ileum
This study aimed to investigate the effects of oxaliplatin treatment on enteric neurons and EGCs within the mouse ileum. BALB/c mice received repeated intraperitoneal injections of oxaliplatin (3 mg/kg, 3 injections/week). Tissues were collected 3, 7, 14, and 21 days from the commencement of treatment. Decreases in glial fibrillary acidic protein–immunoreactive (IR) EGCs and protein gene product 9.5/β-Tubulin III-IR neurons as well as increase in s100β-IR EGCs after chronic oxaliplatin administration were observed in both the myenteric and submucosal plexi. Changes in EGCs were further observed in cross-sec...
Source: Journal of Histochemistry and Cytochemistry - August 29, 2016 Category: Biochemistry Authors: Robinson, A. M., Stojanovska, V., Rahman, A. A., McQuade, R. M., Senior, P. V., Nurgali, K. Tags: Articles Source Type: research