Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction
CONCLUSION: We introduce a standardized, more accurate protocol for assessing hemolytic activity using defibrinated human red blood cells. This approach, facilitated by the increased commercial availability of de-identified human blood and defibrination methods, offers a robust tool for evaluating toxicity of emerging drug compounds, especially AMPs.PMID:38604530 | DOI:10.1016/j.jim.2024.113670 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 11, 2024 Category: Allergy & Immunology Authors: Ashley M Carpenter Monique L van Hoek Source Type: research
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction
CONCLUSION: We introduce a standardized, more accurate protocol for assessing hemolytic activity using defibrinated human red blood cells. This approach, facilitated by the increased commercial availability of de-identified human blood and defibrination methods, offers a robust tool for evaluating toxicity of emerging drug compounds, especially AMPs.PMID:38604530 | DOI:10.1016/j.jim.2024.113670 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 11, 2024 Category: Allergy & Immunology Authors: Ashley M Carpenter Monique L van Hoek Source Type: research
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction
CONCLUSION: We introduce a standardized, more accurate protocol for assessing hemolytic activity using defibrinated human red blood cells. This approach, facilitated by the increased commercial availability of de-identified human blood and defibrination methods, offers a robust tool for evaluating toxicity of emerging drug compounds, especially AMPs.PMID:38604530 | DOI:10.1016/j.jim.2024.113670 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 11, 2024 Category: Allergy & Immunology Authors: Ashley M Carpenter Monique L van Hoek Source Type: research
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction
CONCLUSION: We introduce a standardized, more accurate protocol for assessing hemolytic activity using defibrinated human red blood cells. This approach, facilitated by the increased commercial availability of de-identified human blood and defibrination methods, offers a robust tool for evaluating toxicity of emerging drug compounds, especially AMPs.PMID:38604530 | DOI:10.1016/j.jim.2024.113670 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 11, 2024 Category: Allergy & Immunology Authors: Ashley M Carpenter Monique L van Hoek Source Type: research
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction
CONCLUSION: We introduce a standardized, more accurate protocol for assessing hemolytic activity using defibrinated human red blood cells. This approach, facilitated by the increased commercial availability of de-identified human blood and defibrination methods, offers a robust tool for evaluating toxicity of emerging drug compounds, especially AMPs.PMID:38604530 | DOI:10.1016/j.jim.2024.113670 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 11, 2024 Category: Allergy & Immunology Authors: Ashley M Carpenter Monique L van Hoek Source Type: research
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction
CONCLUSION: We introduce a standardized, more accurate protocol for assessing hemolytic activity using defibrinated human red blood cells. This approach, facilitated by the increased commercial availability of de-identified human blood and defibrination methods, offers a robust tool for evaluating toxicity of emerging drug compounds, especially AMPs.PMID:38604530 | DOI:10.1016/j.jim.2024.113670 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 11, 2024 Category: Allergy & Immunology Authors: Ashley M Carpenter Monique L van Hoek Source Type: research
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment
J Immunol Methods. 2024 Apr 7;529:113671. doi: 10.1016/j.jim.2024.113671. Online ahead of print.NO ABSTRACTPMID:38593868 | DOI:10.1016/j.jim.2024.113671 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 9, 2024 Category: Allergy & Immunology Authors: Hinpetch Daungsupawong Viroj Wiwanitkit Source Type: research
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment
J Immunol Methods. 2024 Apr 7:113671. doi: 10.1016/j.jim.2024.113671. Online ahead of print.NO ABSTRACTPMID:38593868 | DOI:10.1016/j.jim.2024.113671 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 9, 2024 Category: Allergy & Immunology Authors: Hinpetch Daungsupawong Viroj Wiwanitkit Source Type: research
Protein G affinity chromatography is an underrated but very potent purification method for a broad range of species-independent and tag-less Fab-fragments
J Immunol Methods. 2024 Apr 4:113669. doi: 10.1016/j.jim.2024.113669. Online ahead of print.ABSTRACTBecause of their superior properties for certain biological applications small antibody derivatives like fragment of antigen binding (Fab) have found widespread use in basic research and as therapeutics. However, generation of Fab-fragments is still a rather complex matter, reflected by the fact that a variety of methods and purification techniques are necessary for the production of all the different classes of Fab-fragments (kappa/lambda light chains, type of species). Here we demonstrate that Fab-fragments derived from si...
Source: Journal of Immunological Methods - April 6, 2024 Category: Allergy & Immunology Authors: Daniel Stern Paulin Dettmann Brigitte G Dorner Hans Werner Mages Source Type: research
Protein G affinity chromatography is an underrated but very potent purification method for a broad range of species-independent and tag-less Fab-fragments
J Immunol Methods. 2024 Apr 4:113669. doi: 10.1016/j.jim.2024.113669. Online ahead of print.ABSTRACTBecause of their superior properties for certain biological applications small antibody derivatives like fragment of antigen binding (Fab) have found widespread use in basic research and as therapeutics. However, generation of Fab-fragments is still a rather complex matter, reflected by the fact that a variety of methods and purification techniques are necessary for the production of all the different classes of Fab-fragments (kappa/lambda light chains, type of species). Here we demonstrate that Fab-fragments derived from si...
Source: Journal of Immunological Methods - April 6, 2024 Category: Allergy & Immunology Authors: Daniel Stern Paulin Dettmann Brigitte G Dorner Hans Werner Mages Source Type: research
Protein G affinity chromatography is an underrated but very potent purification method for a broad range of species-independent and tag-less Fab-fragments
J Immunol Methods. 2024 Apr 4:113669. doi: 10.1016/j.jim.2024.113669. Online ahead of print.ABSTRACTBecause of their superior properties for certain biological applications small antibody derivatives like fragment of antigen binding (Fab) have found widespread use in basic research and as therapeutics. However, generation of Fab-fragments is still a rather complex matter, reflected by the fact that a variety of methods and purification techniques are necessary for the production of all the different classes of Fab-fragments (kappa/lambda light chains, type of species). Here we demonstrate that Fab-fragments derived from si...
Source: Journal of Immunological Methods - April 6, 2024 Category: Allergy & Immunology Authors: Daniel Stern Paulin Dettmann Brigitte G Dorner Hans Werner Mages Source Type: research
Magnetic CAR T cell purification using an anti-G4S linker antibody
J Immunol Methods. 2024 Apr 2:113667. doi: 10.1016/j.jim.2024.113667. Online ahead of print.ABSTRACTChimeric antigen receptor (CAR) redirected T cells are successfully employed in the combat against several hematological malignancies, however, are often compromised by low transduction rates making refinement of the CAR T cell products necessary. Here, we report a broadly applicable enrichment protocol relying on marking CAR T cells with an anti-glycine4-serine (G4S) linker antibody followed by magnetic activated cell sorting (MACS). The protocol is broadly applicable since the G4S peptide is an integral part of the vast ma...
Source: Journal of Immunological Methods - April 4, 2024 Category: Allergy & Immunology Authors: Dennis Christoph Harrer Sin-Syue Li Marcell Kaljanac Valerie Bezler Markus Barden Hong Pan Wolfgang Herr Hinrich Abken Source Type: research
Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles
This study provides insights into complement activation at the nanosurface level, offering a valuable tool for nanomedicine researchers in the field.PMID:38574804 | DOI:10.1016/j.jim.2024.113668 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 4, 2024 Category: Allergy & Immunology Authors: Yue Li Andrew Monte Layne Dylla S Moein Moghimi Dmitri Simberg Source Type: research
Simultaneous determination of inflammatory factors SAA and LTF based on stable element labeling and inductively coupled plasma mass spectrometry to aid in the diagnosis of infection
CONCLUSION: The quantitative duplex immunoassay for SAA/LTF has high accuracy, good precision, and high specificity, which meets the clinical testing requirements and can be widely used in clinical practice.PMID:38574805 | DOI:10.1016/j.jim.2024.113666 (Source: Journal of Immunological Methods)
Source: Journal of Immunological Methods - April 4, 2024 Category: Allergy & Immunology Authors: Hairong Tang Gongwei Sun Ying Xu Shasha Men Wencan Jiang Chengbin Wang Source Type: research
Magnetic CAR T cell purification using an anti-G4S linker antibody
J Immunol Methods. 2024 Apr 2:113667. doi: 10.1016/j.jim.2024.113667. Online ahead of print.ABSTRACTChimeric antigen receptor (CAR) redirected T cells are successfully employed in the combat against several hematological malignancies, however, are often compromised by low transduction rates making refinement of the CAR T cell products necessary. Here, we report a broadly applicable enrichment protocol relying on marking CAR T cells with an anti-glycine4-serine (G4S) linker antibody followed by magnetic activated cell sorting (MACS). The protocol is broadly applicable since the G4S peptide is an integral part of the vast ma...
Source: Journal of Immunological Methods - April 4, 2024 Category: Allergy & Immunology Authors: Dennis Christoph Harrer Sin-Syue Li Marcell Kaljanac Valerie Bezler Markus Barden Hong Pan Wolfgang Herr Hinrich Abken Source Type: research
