Journal of Industrial Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Multi-modular metabolic engineering and efflux engineering for enhanced lycopene production in recombinant Saccharomyces cerevisiae
J Ind Microbiol Biotechnol. 2024 Apr 15:kuae015. doi: 10.1093/jimb/kuae015. Online ahead of print.ABSTRACTLycopene has been widely used in the food industry and medical field due to its antioxidant, anti-cancer, and anti-inflammatory properties. However, achieving efficient manufacture of lycopene using chassis cells on an industrial scale remains a major challenge. Herein, we attempted to integrate multiple metabolic engineering strategies to establish an efficient and balanced lycopene biosynthetic system in Saccharomyces cerevisiae. First, the lycopene synthesis pathway was modularized to sequentially enhance the metabo...
Source: Journal of Industrial Microbiology and Biotechnology - April 15, 2024 Category: Microbiology Authors: Guangxi Huang Jiarong Li Jingyuan Lin Changqing Duan Guoliang Yan Source Type: research

Application of the surface engineered recombinant Escherichia coli to the industrial battery waste solution for lithium recovery
J Ind Microbiol Biotechnol. 2024 Apr 4:kuae012. doi: 10.1093/jimb/kuae012. Online ahead of print.ABSTRACTEscherichia coli were engineered to selectively adsorb and recover lithium from the environment by employing bacterial cell surface display strategy. Lithium binding peptide LBP1 was integrated to the Escherichia coli membrane protein OmpC. The effect of environmental conditions on the adsorption of lithium by recombinant strain was evaluated, and lithium particles on cellular surface was analysed by FE-SEM and XRD. To elevate the lithium adsorption, dimeric, trimeric and tetrameric repeats of the LBP1 peptide was const...
Source: Journal of Industrial Microbiology and Biotechnology - April 4, 2024 Category: Microbiology Authors: Jaehoon Jeong Vidhya Selvamani Murali Kannan Maruthamuthu Kulandaisamy Arulsamy Soon Ho Hong Source Type: research

Harnessing regulatory networks in Actinobacteria for natural product discovery
J Ind Microbiol Biotechnol. 2024 Apr 3:kuae011. doi: 10.1093/jimb/kuae011. Online ahead of print.ABSTRACTMicrobes typically live in complex habitats, where they need to rapidly adapt to continuously changing growth conditions. To do so, they produce an astonishing array of natural products with diverse structures and functions. Actinobacteria stand out for their prolific production of bioactive molecules, including antibiotics, anticancer agents, antifungals and immunosuppressants. Attention has been directed especially towards identification of the compounds they produce and mining of the large diversity of biosynthetic g...
Source: Journal of Industrial Microbiology and Biotechnology - April 3, 2024 Category: Microbiology Authors: Hannah E Augustijn Anna M Roseboom Marnix H Medema Gilles P van Wezel Source Type: research

Using design of experiments to guide genetic optimization of engineered metabolic pathways
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae010. doi: 10.1093/jimb/kuae010.ABSTRACTDesign of experiments (DoE) is a term used to describe the application of statistical approaches to interrogate the impact of many variables on the performance of a multivariate system. It is commonly used for process optimization in fields such as chemical engineering and material science. Recent advances in the ability to quantitatively control the expression of genes in biological systems open up the possibility to apply DoE for genetic optimization. In this review targeted to genetic and metabolic engineers, we introduce several approa...
Source: Journal of Industrial Microbiology and Biotechnology - March 15, 2024 Category: Microbiology Authors: Seonyun Moon Anna Saboe Michael J Smanski Source Type: research