Investigation of liquid chromatography –mass spectrometry analysis of a peptide aldehyde SJA6017 with identifying its hemiacetal, gem‐diol, and enol ether
This study provides the information needed to understand peptide aldehyde be havior at various steps of preclinical in vitro studies in the presence of methanol. It has assisted in the development of the SJA6017 bioanalysis method and will also aid in the development of bioanalysis methods for similar peptide aldehydes. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 25, 2024 Category: Chemistry Authors: Zhiyang Zack Zou,
Ming ‐jie Han Tags: RESEARCH ARTICLE Source Type: research
In vitro study on the competitive reactions between arsenite and selenite with glutathione
In this study, we aimed to investigate the antagonistic or synergistic effects of As and Se on the binding mechanism of Se and As with GSH at pH 3.0, 5.0, or 6.5. The interaction of As and Se in Se(SG)2 + As(III) or As(SG)3 + Se(IV) binary systems and As(III) + Se(IV) + GSH ternary system were examined depending on their ratios via liquid chromatography diode array detector/electrospray mass spectrometry (LC-DAD/MS) and liquid chromatography–electrospray ionization–tandem mass spectrometry (LC-ESI-MS/MS ). The results showed that the formation of As(GS)3 was not detected in the As(III) + Se(SG)2 bi...
Source: Journal of Mass Spectrometry - April 25, 2024 Category: Chemistry Authors: Canan H öçük Özkan,
Mehmet Atakay,
Bekir Salih,
Gülay Ertaş Tags: RESEARCH ARTICLE Source Type: research
Innovative direct introduction ‐ion mobility–mass spectrometry (DI‐IM‐MS) approach for fast and robust isomer‐specific quantification in a complex matrix: Application to 2′‐fucosyllactose (2’‐FL) in breast milk
AbstractIdentification and specific quantification of isomers in a complex biological matrix by mass spectrometry alone is not an easy task due to their identical chemical formula and therefore their same mass-to-charge ratio (m/z). Here, the potential of direct introduction combined with ion mobility –mass spectrometry (DI-IM-MS) for rapid quantification of isomers as human milk oligosaccharides (HMOs) was investigated. Differences in HMO profiles between various analyzed breast milk samples were highlighted using the single ion mobility monitoring (SIM2) acquisition for high ion mobility resolution detection. Furthermo...
Source: Journal of Mass Spectrometry - April 24, 2024 Category: Chemistry Authors: Estelle Rathahao ‐Paris,
Sarah Abdoun,
Alain Paris,
Blanche Guillon,
Eric Venot,
François Fenaille,
Karine Adel‐Patient,
Sandra Alves Tags: RESEARCH ARTICLE Source Type: research
From molecules to visuals: Empowering drug discovery and development with mass spectrometry imaging
AbstractOver the past three decades, mass spectrometry imaging (MSI) has emerged as a valuable tool for the spatial localization of drugs and metabolites directly from tissue surfaces without the need for labels. MSI offers molecular specificity, making it increasingly popular in the pharmaceutical industry compared to conventional imaging techniques like quantitative whole-body autoradiography (QWBA) and immunohistochemistry, which are unable to distinguish parent drugs from metabolites. Across the industry, there has been a consistent uptake in the utilization of MSI to investigate drug and metabolite distribution patter...
Source: Journal of Mass Spectrometry - April 24, 2024 Category: Chemistry Authors: Bingming Chen,
Marissa Vavrek,
Mark T. Cancilla Tags: PERSPECTIVE Source Type: research
A general, most basic rule for ion dissociation: Ionized molecules
AbstractHerein we revisit a basic rule for the interpretation of ion chemistry of ionized molecules, first proposed by the pioneers of MS spectra interpretation, but somewhat overlooked over the years. This rule states that, when rationalizing or predicting the dissociation chemistry of an ionized molecule (M+.), a model analog to the “mobile proton model,” that is, a “mobile electron model” via “e--jumping ” should be considered. Ground-state M+. is indeed the first species to be considered, but “e--jumping ” may eventually lead to other more energetic electromers—ionized molecules that differ only in th...
Source: Journal of Mass Spectrometry - April 20, 2024 Category: Chemistry Authors: Adriano Reis,
Marcos N. Eberlin Tags: LETTER Source Type: research
Imaging with mass spectrometry: Which ionization technique is best?
AbstractThe use of mass spectrometry (MS) to acquire molecular images of biological tissues and other substrates has developed into an indispensable analytical tool over the past 25 years. Imaging mass spectrometry technologies are widely used today to study the in situ spatial distributions for a variety of analytes. Early MS images were acquired using secondary ion mass spectrometry and matrix-assisted laser desorption/ionization. Researchers have also designed and develop ed other ionization techniques in recent years to probe surfaces and generate MS images, including desorption electrospray ionization (DESI), nanoD...
Source: Journal of Mass Spectrometry - April 17, 2024 Category: Chemistry Authors: Boone M. Prentice Tags: PERSPECTIVE Source Type: research
Enhancing drug development and clinical studies with patient ‐centric sampling using microsampling techniques: Opportunities, challenges, and insights into liquid chromatography‐mass spectrometry strategies
AbstractMicrosampling has revolutionized pharmaceutical drug development and clinical research by reducing sample volume requirements, allowing sample collection at home or nontraditional sites, minimizing animal and patient burden, and enabling more flexible study designs. This perspective paper discusses the transformative impact of microsampling and patient-centric sampling (PCS) techniques, emphasizing their advantages in drug development and clinical trials. We highlight the integration of liquid chromatography-mass spectrometry (LC –MS) strategies for analyzing PCS samples, focusing on our research experience and a...
Source: Journal of Mass Spectrometry - April 16, 2024 Category: Chemistry Authors: Zhuo Chen,
Christopher C. Goudarzi,
Timothy W. Sikorski,
Naidong Weng Tags: PERSPECTIVE Source Type: research
Removal of isobaric interference using pseudo ‐multiple reaction monitoring and energy‐resolved mass spectrometry for the isotope dilution quantification of a tryptic peptide
AbstractEnergy-resolved mass spectrometry (ERMS) and an isotopically labelled internal standard were successfully combined to accurately quantify a tryptic peptide despite the presence of an isobaric interference. For this purpose, electrospray ionisation tandem mass spectrometry (ESI-MS/MS) experiments were conducted into an ion trap instrument using an unconventional 8 m/z broadband isolation window, which encompassed both the tryptic peptide and its internal standard. Interference removal was assessed by determining an excitation voltage that was high enough to maintain a constant value for the analyte/internal standa...
Source: Journal of Mass Spectrometry - April 13, 2024 Category: Chemistry Authors: Alicia Maroto,
Dany Jeanne dit Fouque,
R émy Lartia,
Antony Memboeuf Tags: RESEARCH ARTICLE Source Type: research
From the grapevine to the glass: A wine metabolomics tale by FT ‐ICR‐MS
AbstractWine is one of the most consumed beverages around the world. Its unique characteristics arise from numerous processes, from the selection of grapevine varieties and grapes, the effect of the terroir and geographical origin, through the biochemical process of fermentation by microorganisms, until its aging. All molecules found in wine define its chemical fingerprint and can be used to tell the story of its origin, production, authenticity and quality. Wine's chemical composition can be characterized using an untargeted metabolomics approach based on extreme resolution mass spectrometry. Fourier transform ion cyclotr...
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Marta Sousa Silva,
M ónica Soeiro,
Carlos Cordeiro Tags: REVIEW ARTICLE Source Type: research
Simultaneous quantitation of 15 bioactive components in Yupingfeng granules by LC –MS/MS
This study aimed to establish a simple and rapid method by using liquid chromatography –tandem mass spectrometry (LC–MS/MS) to determine 15 bioactive compounds in YPFG. The experimental parameters including extraction methods, extraction solvents, extraction time, solid–liquid ratio, and LC–MS/MS condition were optimized. The linearity, precision, repeatability, stability, and recovery of the established method were evaluated. The contents of 15 bioactive compounds in seven batches of YPFG samples were analyzed by the established method and the results were compared with the values determined by HPLC. The optimal e...
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Le ‐yi Huang,
Dao‐feng Chen,
Tong Wu,
Yong‐jian Gao Tags: RESEARCH ARTICLE Source Type: research
High ‐resolution ion mobility separations coupled to mass spectrometry: What's next?
AbstractHerein, I provide a personal perspective on high-resolution multipass ion mobility spectrometry-mass spectrometry (IMS-MS), with a specific emphasis on cyclic (cIMS) and structures for lossless ion manipulations (SLIM IMS)-based separations. My overarching goal for this perspective was to detail what I believe will be the key important areas in which IMS-MS will help shape the bioanalytical community and especially omics-based research. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Gabe Nagy Tags: PERSPECTIVE Source Type: research
Investigating direct current potentials that affect native protein conformation during trapped ion mobility spectrometry –mass spectrometry
AbstractTrapped ion mobility spectrometry –time-of-flight mass spectrometry (TIMS-TOFMS) has emerged as a tool to study protein conformational states. In TIMS, gas-phase ions are guided across the IM stages by applying direct current (DC) potentials (D1–6), which, however, might induce changes in protein structures through collisional a ctivation. To define conditions for native protein analysis, we evaluated the influence of these DC potentials using the metalloenzyme bovine carbonic anhydrase (BCA) as primary test compound. The variation of DC potentials did not change BCA-ion charge and heme content but affected (re...
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Robert L. C. Voeten,
Hany A. Majeed,
Tijmen S. Bos,
Govert W. Somsen,
Rob Haselberg Tags: RESEARCH ARTICLE Source Type: research
Perspective: The complex relationship between charge, mobility, and gas ‐phase protein structure
AbstractIon mobility spectrometry coupled to mass spectrometry (IMS/MS) is a widely used tool for biomolecular separations and structural elucidation. The application of IMS/MS has resulted in exciting developments in structural proteomics and genomics. This perspective gives a brief background of the field, addresses some of the important issues in making structural measurements, and introduces complementary techniques. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Ian K. Webb Tags: PERSPECTIVE Source Type: research
The future role of mass spectrometry in proteomics: Embracing new technologies and building bridges for higher impact
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Lindsay K. Pino Tags: PERSPECTIVE Source Type: research
Issue Information
No abstract is available for this article. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 10, 2024 Category: Chemistry Tags: ISSUE INFORMATION Source Type: research
