Journal of Microbiology and BiotechnologyThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

This page shows you the latest items in this publication.

Pages: 1 2 3 4 5 6 7 8

362 records returned

A Novel pH-Stable, Bifunctional Xylanase Isolated from a Deep-Sea Microorganism, Demequina sp. JK4.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A genomic library was constructed to clone a xylanase gene (Mxyn10) from Demequina sp. JK4 isolated from a deep sea. Mxyn10 encoded a 471 residue protein with a calculated molecular mass of 49 kDa. This protein showed the highest sequence identity (70%) with the xylanase from Streptomyces lividans. Mxyn10 contains a catalytic domain that belongs to the glycoside hydrolase family 10 (GH10) and a carbohydrate-binding module (CBM) belonging to family 2. The optimum pH and temperature for enzymatic activity were pH 5.5 and 55 degrees , respectively. Mxyn10 exhibited good pH stability, remaining stable after treatment with ...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Meng X, Shao Z, Hong Y, Lin L, Li C, Liu Z Tags: J Microbiol Biotechnol Source Type: journals

Novel Low-Temperature-Active Phytase from Erwinia carotovora var.carotovota ACCC 10276.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A phytase with high activity at low temperatures has great potential for feed applications, especially in aquaculture. Therefore, this study used a degenerate PCR and TAIL PCR to clone a phytase gene from Erwinia carotovora var. carotovota, the cause of soft rot of vegetables in the ground or during cold storage. The full-length 2.5-kb fragment included an open reading frame of 1,302 bp and encoded a putative phytase of 45.3 kDa with a 50% amino acid identity to the Klebsiella pneumoniae phytase. The phytase contained the active site RHGXRXP and HD sequence motifs that are typical of histidine acid phosphatases. The en...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Huang H, Luo H, Wang Y, Fu D, Shao N, Yang P, Meng K, Yao B Tags: J Microbiol Biotechnol Source Type: journals

Biochemical Characteristics and Function of a Fucosyltransferase Encoded by ste7 in Ebosin Biosynthesis of Streptomyces sp. 139.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A novel exopolysaccharide named Ebosin was produced by Streptomyces sp. 139, with medicinal activity. Its biosynthesis gene cluster (ste) has been previously identified. For the functional study of the ste7 gene in Ebosin biosynthesis, it was disrupted with a double crossover via homologous recombination. The monosaccharide composition of EPS- 7m produced by the mutant strain Streptomyces sp. 139 (ste7-) was found altered from that of Ebosin, with fucose decreasing remarkably. For biochemical characterization of Ste7, the ste7 gene was cloned and expressed in Escherichia coli BL21. With a continuous coupled spectrophot...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Chang M, Bai LP, Shan JJ, Jiang R, Zhang Y, Guo LH, Zhang R, Li Y Tags: J Microbiol Biotechnol Source Type: journals

Production of Gamma-Linolenic Acid in Pichia pastoris by Expression of a Delta-6 Desaturase Gene from Cunninghamella echinulata.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Gamma-linolenic acid (GLA, C18:3 delta6,9,12) is synthesized by a delta-6 fatty acid desaturase using linoleic acid (LA, C18:2 delta9,12) as a substrate. To enable the production of GLA in the conventional yeast Pichia pastoris, we have isolated a cDNA encoding the delta-6 fatty acid desaturase from Cunninghamella echinulata MIAN6 and confirmed its function by heterogeneous expression in P. pastoris. Sequence analysis indicated that this cDNA sequence has an open reading frame of 1,404 bp, which encodes a 52 kDa peptide of 468 amino acids. This sequence has 64% identity to the previously reported delta-6 fatty acid des...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Wan X, Zhang Y, Wang P, Huang F, Chen H, Jiang M Tags: J Microbiol Biotechnol Source Type: journals

Pathway Analysis in HEK 293T Cells Overexpressing HIV-1 Tat and Nucleocapsid.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The human immunodeficiency virus (HIV)-1 protein Tat acts as a transcription transactivator that stimulates expression of the infected viral genome. It is released from infected cells and can similarly affect neighboring cells. The nucleocapsid is an important protein that has a related significant role in early mRNA expression, and which contributes to the rapid viral replication that occurs during HIV-1 infection. To investigate the interaction between the Tat and nucleocapsid proteins, we utilized cDNA microarrays using pTat and flag NC cotransfection in HEK 293T cells and reverse transcription-polymerase chain reac...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Lee MJ, Park JH Tags: J Microbiol Biotechnol Source Type: journals

Proteomic Analysis in ob/ob Mice Before and After Hypoglycemic Polysaccharide Treatments.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In an attempt to discover novel biomarker proteins in type 2 diabetes prognosis, we investigated the influence of hypoglycemic extracellular polysaccharides (EPS) obtained from the macrofungus Tremella fuciformis on the differential levels of plasma proteins in ob/ob mice using two-dimensional gel electrophoresis (2-DE). The 2-DE analysis demonstrated that 92 spots from about 900 visualized spots were differentially regulated, of which 40 spots were identified as principal diabetes-associated proteins. By comparing control with EPS-fed mice, we found that at least six proteins were significantly altered in ob/ob mice, ...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Sang Woo K, Hwang HJ, Baek YM, Hwang HS, Yun JW Tags: J Microbiol Biotechnol Source Type: journals

Cadaverine is transported into Vibrio vulnificus through its CadB in alkaline environment.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The exogenously-added cadaverine is effective in protecting Vibrio vulnificus from methyl viologen (MV)-induced superoxide stress at pH 8.5. Such a protective effect by cadaverine was not observed at pH 7.5. Consistently, the accumulated level of intracellular cadaverine at pH 8.5 is approximately four times as much as that of the control cell at pH 7.5. Cadaverine accumulation is not affected by MV. The protection of V. vulnificus by cadaverine from superoxide stress was abolished when cadB coding for lysine-cadaverine antiporter was interrupted. However, the cadaverine-mediated protection was complemented with cadB D...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Kang IH, Kim EJ, Lee JK Tags: J Microbiol Biotechnol Source Type: journals

Acetate Consumption Activity Directly Determines the Level of Acetate Accumulation During Escherichia coli W3110 Growth.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Escherichia coli excretes acetate during aerobic growth on glycolytic carbon sources, which has been explained as an overflow metabolism when the carbon flux into the cell exceeds the capacity of central metabolic pathways. Nonacetogenic growth of E. coli on gluconeogenic carbon sources like succinate or in carbon-limited slow growth conditions is believed an evidence for the explanation. However, we found that a strain defected in the acs (acetyl Co-A synthetase) gene, the product of which is involved in scavenging acetate, accumulated acetate even in succinate medium and in carbon-limited low growth rate condition, w...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Shin S, Chang DE, Pan JG Tags: J Microbiol Biotechnol Source Type: journals

Antitumor Components from Naematoloma fasciculare.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The bioassay-guided fractionation of MeOH extract from Naematoloma fasciculare afforded a petroleum ether fraction (NFPF) and four known compounds, which showed good antitumor activities to inhibit MCF-7 cell line proliferation in vitro and tumor growth in H22 implanted mice in vivo. In addition, a number of unsaturated aliphatic acids were identified in NFPF by GC analysis. These results showed that NFPF inhibits tumor growth through the activity of unsaturated aliphatic acids together with two active compounds, ergosterol peroxide (1: 62.17 mg/g in NFPF) and ergosterol (2: 3.13 mg/g in NFPF), and indicate the potenti...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Yan D, Bao HY, Bau T, Li Y, Kim YH Tags: J Microbiol Biotechnol Source Type: journals

Clitocybin D, a Novel Human Neutrophil Elastase Inhibitor from the Culture Broth of Clitocybe aurantiaca.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clitocybin D, a novel human neutrophil elastase inhibitor, was isolated from the culture broth of Clitocybe aurantiaca. This compound was purified by solvent extraction, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. The compound was determined to be 4-(4,6-dihydroxy-3-methoxy-3Hisoindol- 1-yl)-benzoic acid on the basis of 1D and 2D NMRs and MS spectroscopic analysis. Analysis of the human neutrophil elastase (HNE) inhibitory activity of the isolated compound revealed that it showed significan HNE inhibitory activity with an IC50 value of 17.8 micronM. PMID: 19884771 [...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Young-Hee K, Ryoo IJ, Choo SJ, Xu GH, Lee S, Seok SJ, Bae K, Yoo ID Tags: J Microbiol Biotechnol Source Type: journals

Effects of Nutrients on Quorum Signals and Secondary Metabolite Productions of Burkholderia sp. O33.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Several bioactive metabolites, including pyrrolnitrin, Nacylhomoserine lactones, and polyhydroxyalkanoates were isolated from Burkholderia sp. O33. Effects of various nutrients, including sugars, gluconolactone, glycerol, tryptophan, chloride, and zinc were investigated in relation to the production of these metabolites. Logarithmic increase of pyrrolnitrin was observed between 2-5 days and reached a maximum at 7-10 days. Tryptophan concentration reached the maximum at 3 days, whereas 7-chlorotryptophan was gradually increased throughout the studies. Among various carbon sources, gluconolactone, trehalose, and glycerol...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Keum YS, Lee YJ, Lee YH, Kim JH Tags: J Microbiol Biotechnol Source Type: journals

Microbial Transformation of a Monoterpene, Geraniol, by the Marinederived Fungus Myrothecium sp.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Geraniol (1) is the biogenetic precursor of a number of monoterpenes. We tested various marine-derived microorganisms to determine their ability to biotransform 1. Only Myrothecium sp. was capable of transforming 1 into its oxidized derivative, 1,7-dihydroxy-3,7-dimethyl-(E)-oct- 2-ene (2). The structure of the metabolite obtained was assigned on the basis of detailed spectroscopic data analyses. PMID: 19884773 [PubMed - in process] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Alain S L, Yang G, Nenkep VN, Siwe XN, Feng Z, Khong TT, Choi HD, Kang JS, Son BW Tags: J Microbiol Biotechnol Source Type: journals

Production of Lactosucrose from Sucrose and Lactose by a Levansucrase from Zymomonas mobilis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were 23 degrees , pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/ml of reaction mixture. Under these conditions, the lactosucrose conversion efficiency was 28.5%. The product was purified and confirmed to be O-beta-D-galactopyranosyl-(1-->4)-O-beta-D-glucopyranosyl- (1-->2)-beta-D-fructofuranoside, or lactosucrose. A mixed-enzyme system containing a levansucrase and a glucose oxidase was applied in order ...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Han WC, Byun SH, Kim MH, Sohn EH, Lim JD, Um BH, Kim CH, Kang SA, Jang KH Tags: J Microbiol Biotechnol Source Type: journals

Ethanol Production from Rice Winery Waste-Rice Wine Cake by Simultaneous Saccharification and Fermentation without Cooking.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ethanol production by simultaneous saccharification and fermentation (SSF) of low-value rice wine cake (RWC) without cooking was investigated. RWC is the filtered solid waste of fermented rice wine mash and contains 53% of raw starch. RWC slurry was mixed with raw-starch-digesting enzyme of Rhizopus sp. and yeast for SSF. The yeast strain used was selected from 300 strains for RWC fermentation and identified as Saccharomyces cerevisiae KV25. High efficiency (94%) of ethanol production was achieved at optimal condition of uncooked RWC slurry containing 23.03% of starch. The optimal SSF condition determined was 1.125 uni...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Vu VH, Kim K Tags: J Microbiol Biotechnol Source Type: journals

Analysis of the Involvement of Chitin-Binding Domain of ChiCW in Antifungal Activity, and Engineering a Novel Chimeric Chitinase with High Enzyme and Antifungal Activities.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An antifungal chitinase, ChiCW, produced by Bacillus cereus 28-9 is effective against conidial germination of Botrytis elliptica, the causal agent of lily leaf blight. ChiCW as a modular enzyme consists of a signal peptide, a catalytic domain, a fibronectin type-III-like domain, and a chitinbinding domain. When two C-terminal domains of ChiCW were truncated, ChiCW¥ÄFC (lacking the chitin-binding domain and fibronectin type III-like domain) lost its antifungal activity. Since ChiCW¥ÄC (lacking the chitinbinding domain) could not be expressed in Escherichia coli as ChiCW¥&Aum...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Huang CJ, Guo SH, Chung SC, Lin YJ, Chen CY Tags: J Microbiol Biotechnol Source Type: journals

Characterization of Surface Layer Proteins in Lactobacillus crispatus Isolate ZJ001.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, S-layer genes were sequenced and expressed in E. coli to characterize the function of S-layer proteins with this particular strain. L. crispatus ZJ001 harbored two Slayer genes slpA and slpB, and only slpA gene was expressed in the bacterium, as revealed by RT-PCR and immunoassays. The mature SlpA showed 47% amino acid sequence identity to SlpB. The SlpA and SlpB of L. crispatus ZJ001 were highly homologous at the C-terminal region to other Lactobacillus S-layer proteins, but were substantially variable at N-terminal and middle regions. Electron microscopic analysis indicated that His-slpA expressed in E. co...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Chen X, Chen Y, Li X, Chen N, Fang W Tags: J Microbiol Biotechnol Source Type: journals

Purification and Characterization of Endo-beta-1,4 Mannanase from Aspergillus niger gr for Application in Food Processing Industry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A thermostable extracellular beta-mannanase from the culture supernatant of a fungus Aspergillus niger gr was purified to homogeneity. SDS-PAGE of the purified enzyme showed a single protein band of molecular mass 66 kDa. The beta- mannanase exhibited optimum catalytic activity at pH 5.5 and 55 degrees . It was thermostable at 55 degrees , and retained 50% activity after 6 h at 55deg;. The enzyme was stable at a pH range of 3.0 to 7.0. The metal ions Hg2+, Cu2+, and Ag2+ inhibited complete enzyme activity. The inhibitors tested, EDTA, PMSF, and 1,10-phenanthroline, did not inhibit the enzyme activity. N-Bromosuccinimid...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Naganagouda K, Salimath PV, Mulimani VH Tags: J Microbiol Biotechnol Source Type: journals

Medium Optimization and Application of an Affinity Column Chromatography for Streptomyces griseus Trypsin Production from the Recombinant Streptomyces griseus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The expression vector pWHM3-TR1R2, which contains sprT encoding Streptomyces griseus trypsin (SGT) and two positive regulatory genes (sgtR1 and sgtR2), was introduced into S. griseus IFO13350 and the productivity of SGT by the transformant was investigated in various media. Among the tested media, Ferm-0 gave 1.4 times more trypsin activity than C5/L medium. In addition, replacement of 2% glucose and 1% skim milk in Ferm-0 medium with 2% dextrin and 1% tryptone (named as Ferm-II medium) yielded significantly enhanced trypsin activity, by 4.1-fold, than that of Ferm-0. For simplifying the purification process, the cultu...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Chi WJ, Song JH, Oh EA, Park SW, Chang YK, Kim ES, Hong SK Tags: J Microbiol Biotechnol Source Type: journals

Purification and characterization of neoagarotetraose from hydrolyzed agar.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Whitening effect, tyrosinase inhibitions, and cytotoxicity of neoagarotetraose were measured after purification from hydrolyzed agar by gel filtration chromatography. In melanoma B16F10 cells, melanin content of neoagarotetraose-treated cells was as same as that treated by kojic acid or arbutin. In addition, tyrosinase of melanoma cells was strongly inhibited by neoagarotetraose at a concentration of 1 microng/ml and similarly inhibited at 10 and 100 microng/ml compared to those by arbutin or kojic acid. The activity of mushroom tyrosinase showed a 38% inhibition by neoagarotetraose at 1 microng/ml, and this inhibitory...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Jang MK, Lee DG, Kim NY, Yu KH, Jang HJ, Lee SW, Jang HJ, Lee YJ, Lee SH Tags: J Microbiol Biotechnol Source Type: journals

Effect of Amylose Content in Corn Starch Modification by Thermus aquatiqus 4-alpha-Glucanotransferase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Corn starches with different amylose contents were enzymatically modified using Thermus aquaticus 4-alpha-glucanotransferase (TAalphaGTase). On treating, chain length distribution of the product became broader (degree of polymerization; DP 3-40) after isoamylolysis when compared to the untreated corn starch. In addition, a variety of different size cycloamyloses were formed by glucanotransferring activity of TAalphaGTase. Cycloamyloses (CAs) with DP 5-40 were detectable in all of the TAalphaGTase-treated corn starches. The amount of CAs produced by the enzyme treatment seems to increase as amylose content of starch inc...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Cho KH, Auh JH, Kim JH, Ryu JH, Park KH, Park CS, Yoo SH Tags: J Microbiol Biotechnol Source Type: journals

Use of FT-IR to Identify Enhanced Biomass Production and Biochemical Pool Shifts in the Marine Microalgae, Chlorella ovalis, Cultured in Media Composed of Different Ratios of Deep Seawater and Fermented Animal Wastewater.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Growth rates, photosystem II photosynthesis, and the levels of chlorophyll a and secondary metabolites of Chlorella ovalis were estimated to determine if they were enhanced by the addition of swine urine (BM) or cow compost water (EP) that had been fermented by soil bacteria to deep seawater (DSW) in an attempt to develop media that enabled batch mass culture at lower costs. Growth of C. ovalis in f/2, f/2-EDTA+BM60%, DSW+BM30%, and DSW+EP60% was enhanced and maintained in the log phase of growth for 16 days. The cell densities of C. ovalis in DSW+EP60% (4.1x106 Cells/ml) were higher than those of f/2 (2.9x106 Cells/ml...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Kim MK, Jeune KH Tags: J Microbiol Biotechnol Source Type: journals

Characterization of plant growth-promoting traits of free-living diazotrophic bacteria and their inoculation effects on growth and nitrogen uptake of crop plants.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The search for diverse plant growth-promoting (PGP) diazotrophic bacteria is gaining momentum as efforts are made to exploit them as biofertilizers for various economically important crops. In the present study, 17 diazotrophic strains belonging to eight different genera isolated from rice paddy fields were screened for multiple PGP traits and evaluated for their inoculation effects on canola and rice plants. All of the strains tested positive for 1- aminocyclopropane-1-carboxylate (ACC) deaminase activity and production of indole 3-acetic acid (IAA) and ammonia (NH3). Additionally, four of the strains were able to sol...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Islam MR, Madhaiyan M, Deka Boruah HP, Yim W, Lee G, Saravanan VS, Fu Q, Hu H, Sa T Tags: J Microbiol Biotechnol Source Type: journals

Construction of a Recombinant Bacillus velezensis Strain as an Integrated Control Agent Against Plant Diseases and Insect Pests.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To construct a new recombinant strain of Bacillus velezensis that has antifungal and insecticidal activity via the expression of the insecticidal Bacillus thuringiensis crystal protein, a B. thuringiensis expression vector (pHT1K-1Ac) was generated that contained the B. thuringiensis cry1Ac gene under the control of its endogenous promoter in a minimal E. coli-B. thuringiensis shuttle vector (pHT1K). This vector was introduced into a B. velezensis isolate that showed high antifungal activities against several plant diseases, including rice blast (Magnaporthe grisea), rice sheath blight (Rhizotonia solani), tomato gray ...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Roh JY, Liu Q, Choi JY, Wang Y, Shim HJ, Xu HG, Choi GJ, Kim JC, Je YH Tags: J Microbiol Biotechnol Source Type: journals

Selective Plugging Strategy-Based Microbial-Enhanced Oil Recovery Using Bacillus licheniformis TT33.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The selective plugging strategy of microbial enhanced oil recovery involves the use of microbes that grow and produce exopolymeric substances, which block the high permeability zones of an oil reservoir, thus allowing the water to flow through the low permeability zones leading to increase in oil recovery. Bacillus licheniformis TT33, a hot water spring isolate, is facultatively anaerobic, halotolerant, and thermotolerant. It produces EPS as well as biosurfactant and has a biofilm-forming ability. The viscosity of its cell-free supernatant is 120 mPas at 28oC. Its purified EPS contained 26% carbohydrate and 3% protein....
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Suthar H, Hingurao K, Desai A, Nerurkar A Tags: J Microbiol Biotechnol Source Type: journals

Antimicrobials effective for inhibition of enterohaemorrhagic Escherichia coli strains O26, O111, and O157 and their effects on Shiga toxin releases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Susceptibilities of major enterohaemorrhagic Escherichia coli (EHEC) strains to antimicrobial agents and the cytotoxicity of these agents were examined using a total of 38 strains of E. coli O26, O111, and O157, which are the major serogroups of EHEC. Among the 38 strains, 35, 36, and 36 were susceptible to amikacin, imipenem and norfloxacin, respectively. These antimicrobial agents were further examined to determine their cytotoxicity on Vero cells as well as their effect on the release of Shiga toxins along with trimethoprim/sulfamethoxazole. Each of the E. coli O26, O111, and O157 strains containing both stx1and stx...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Lee JH, Stein B Tags: J Microbiol Biotechnol Source Type: journals

Phoma herbarum as a New Gibberellin-Producing and Plant Growth-Promoting Fungus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Endophytic fungi are known for the production of valuable metabolites, but information on the gibberellin production capacity of this group is limited. We isolated 9 endophytic fungi from the roots of salt-stressed soybean plants and screened them on waito-c rice, in order to identify plant growth promoting fungal strains. The fungal isolate TK- 2-4 gave maximum plant length (20.35 cm) promotion in comparison with wild-type Gibberella fujikuroi (19.5 cm). In a separate experiment, bioassay of TK-2-4 promoted plant length and biomass of soybean cultivar Taegwangkong. The TK-2-4 culture filtrate was analyzed for the pres...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Hamayun M, Khan SA, Khan AL, Rehman G, Sohn EY, Shah AA, Kim SK, Joo GJ, Lee IJ Tags: J Microbiol Biotechnol Source Type: journals

Molecular Mechanism of Plant Growth Promotion and Induced Systemic Resistance to Tobacco Mosaic Virus by Bacillus spp.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bacillus spp., as a type of plant growth-promoting rhizobacteria (PGPR), were studied with regards promoting plant growth and inducing plant systemic resistance. The results of greenhouse experiments with tobacco plants demonstrated that treatment with the Bacillus spp. significantly enhanced the plant height and fresh weight, while clearly lowering the disease severity rating of the tobacco mosaic virus (TMV) at 28 days post-inoculation (dpi). The TMV accumulation in the young non-inoculated leaves was remarkably lower for all the plants treated with the Bacillus spp. An RTPCR analysis of the signaling regulatory gene...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Wang S, Wu H, Qiao J, Ma L, Liu J, Xia Y, Gao X Tags: J Microbiol Biotechnol Source Type: journals

Comparative evaluation of three culture methods for the isolation of mycobacteria from clinical samples.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We assessed the capacity of two liquid-medium culture methods with automated incubation and reading systems (MB/BacT ALERT 3D System and BACTEC MGIT 960 System) and one solid-medium culture method (Löwenstein- Jensen) to detect mycobacteria in different types of clinical samples. Out of 1,770 cultured clinical samples (1,519 of respiratory origin and 251 of nonrespiratory origin), mycobacteria were isolated in 156 samples (135 M. tuberculosis complex, 8 M. chelonae, 6 M. kansasii, 4 M. fortuitum, 2 M. gordonae, and 1 M. marinum) by at least one of the methods used. The BACTEC MGIT 960 System proved to be the most ...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Sorlozano A, Soria I, Roman J, Huertas P, Soto MJ, Piedrola G, Gutierrez J Tags: J Microbiol Biotechnol Source Type: journals

Antibiograms and Molecular Subtypes of Methicillin-Resistant Staphylococcus aureus in Local Teaching Hospital, Malaysia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, no vancomycin-resistant isolate was observed. The Malaysian MDR MRSA isolates were mostly SCCmec type III and negative for PVL. These strains were genetically distinct from the SCCmec type IV strains, which were sensitive to SXT, tetracycline, and erythromycin. Only two strains were SCCmec IV and PVL-positive. The infections in the hospital concerned were probably caused by multiple subtypes of MRSA. PMID: 19884790 [PubMed - in process] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Thong KL, Junnie J, Liew FY, Yusof MY, Hanifah YA Tags: J Microbiol Biotechnol Source Type: journals

Multi-Immunogenic Outer Membrane Vesicles Derived from an MsbBDeficient Salmonella enterica Serovar Typhimurium Mutant.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To develop low endotoxic and multi-immunogenic outer membrane vesicles (OMVs), a deletion mutant of the msbB gene in Salmonella enterica serovar Typhimurium (S. Typhimurium) was used as a source of low endotoxic OMV, and an expression vector of the canine parvovirus (CPV) VP2 epitope fused to the bacterial OmpA protein was constructed and transformed into the Salmonella yenAmsbB mutant. In a lethality test, BALB/c mice injected intraperitoneally with the Salmonella yenAmsbB mutant survived for 7 days, whereas mice injected intraperitoneally with the wild type survived for 3 days. Moreover, all mice inoculated orally wi...
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Lee SR, Kim SH, Jeong KJ, Kim KS, Kim YH, Kim SJ, Kim E, Kim JW, Chang KT Tags: J Microbiol Biotechnol Source Type: journals

Characterization of a 27 kDa Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH51 Isolated from Cheonggukjang.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19884792 [PubMed - in process] (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - October 1, 2009 Category: Biotechnology Authors: Kim GM, Lee AR, Lee KW, Park JY, Chun J, Cha J, Song YS, Kim JH Tags: J Microbiol Biotechnol Source Type: journals

Development and Characterization of New Microsatellite Markers for the Oyster Mushroom (Pleurotus ostreatus).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We developed and characterized 36 polymorphic microsatellite markers for the oyster mushroom (Pleurotus ostreatus). In total, 169 alleles were identified with an average of 4.7 alleles per locus. Values for observed (HO) and expected (HE) heterozygosities ranged from 0.027 to 0.946 and from 0.027 to 0.810, respectively. Nineteen loci deviated from Hardy-Weinberg equilibrium. Significant (P<0.05) excess heterozygosity was observed at nine loci. Linkage disequilibrium (LD) was significant (P<0.05) between pairs of locus alleles. Cluster analysis revealed that five species of genus Pleurotus made a distinct group, a...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Ma KH, Lee GA, Lee SY, Gwag JG, Kim TS, Kong WS, Seo KI, Lee GS, Park YJ Tags: J Microbiol Biotechnol Source Type: journals

Abundance and Structure of Microbial Loop Components (Bacteria and Protists) in Lakes of Different Trophic Status.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The abundance, biomass, size distribution, and taxonomic composition of bacterial and protistan (heterotrophic and autotrophic nanoflagellates and ciliates) communities were investigated in six lakes of Masurian Lake District (north-eastern Poland) differing in trophic state. Samples were taken from the trophogenic water layer during summer stratification periods in the years of 2004 and 2006. Image analysis techniques with fluorescent in situ hybridization (FISH) as well as [3H]-methyl-thymidine incorporation methods were applied to analyze differences in the composition and activity of bacterial communities. The grea...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Ryszard J C, Tomasz A, Kalinowska K, Skowronska A Tags: J Microbiol Biotechnol Source Type: journals

Combination Strategy to Increase Cyclosporin A Productivity by Tolypocladium niveum Using Random Mutagenesis and Protoplast Transformation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The cyclic undecapeptide cyclosporin A (CyA), one of the most valuable immunosuppressive drugs, is produced nonribosomally by a multifunctional cyclosporin synthetase enzyme complex by the filamentous fungus Tolypocladium niveum. To increase CyA productivity by wild-type T. niveum (ATCC 34921), random mutagenesis was first performed using an antifungal agar-plug colony assay (APCA) selection approach. This generated a mutant strain producing more than 9-fold greater CyA than the wild-type strain. Additionally, a foreign bacterial gene, Vitreoscilla hemoglobin gene (VHb), was transformed via protoplast regeneration and ...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Lee MJ, Duong CT, Han K, Kim ES Tags: J Microbiol Biotechnol Source Type: journals

Novel Alkali-Stable, Cellulase-Free Xylanase from Deep-Sea Kocuria sp. Mn22.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A novel xylanase gene, Kxyn, was cloned from Kocuria sp. Mn22, a bacteria isolated from the deep sea of the east Pacific. Kxyn consists of 1,170 bp and encodes a protein of 390 amino acids that shows the highest identity (63%) with a xylanase from Thermobifida fusca YX. The mature protein with a molecular mass of approximately 40 kDa was expressed in Escherichia coli BL21 (DE3). The recombinant Kxyn displayed its maximum activity at 55 degrees and at pH 8.5. The Km, Vmax, and kcat values of Kxyn for birchwood xylan were 5.4mg/ml, 272 micronmol/minmg, and 185.1/s, respectively. Kxyn hydrolyzed birchwood xylan to produce...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Chanjuan L, Hong Y, Shao Z, Lin L, Huang X, Liu P, Wu G, Meng X, Liu Z Tags: J Microbiol Biotechnol Source Type: journals

Isolation and Characterization of the Eicosapentaenoic Acid Biosynthesis Gene Cluster from Shewanella sp. BR-2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Forty-four eicosapentaenoic acid (EPA)-producing microbial strains were isolated from the intestines of marine fishes. Among them, one strain showing a maximum level of EPA (4.78%of total fatty acids) was identified as Shewanella sp. BR-2 on the basis of its 16S rRNA sequence. The EPA content reached a maximum level during the mid-exponential phase of cell growth, and gradually decreased with further growth of the cells. A cosmid DNA including the EPA biosynthesis gene cluster consisting of pfaA-E was isolated from a cosmid library of genomic DNA of Shewanella sp. BR-2, named pCosEPA-BR2. An E. coli clone harboring pCo...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Lee SJ, Seo PS, Kim CH, Kwon O, Hur BK, Seo JW Tags: J Microbiol Biotechnol Source Type: journals

Lipase diversity in glacier soil based on analysis of metagenomic DNA fragments and cell culture.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lipase diversity in glacier soil was assessed by cultureindependent metagenomic DNA fragment screening and confirmed by cell culture experiments. A set of degenerate PCR primers specific for lipases of the hormone-sensitive lipase family was designed based on conserved motifs and used to directly PCR amplify metagenomic DNA from glacier soil. These products were used to construct a lipase fragment clone library. Among the 300 clones sequenced for the analysis, 201 clones encoding partial lipases shared 51-82%identity to known lipases in GenBank. Based on a phylogenetic analysis, five divergent clusters were established...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Yuhong Z, Shi P, Liu W, Meng K, Bai Y, Wang G, Zhan Z, Yao B Tags: J Microbiol Biotechnol Source Type: journals

Identification of Salmonella pullorum Genomic Sequences Using Suppression Subtractive Hybridization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pullorum disease affecting poultry is caused by Salmonella enterica serovar Pullorum and results in severe economic loss every year, especially in countries with a developing poultry industry. The pathogenesis of S. Pullorum is not yet well defined, as the specific virulence factors still need to be identified. Thus, to isolate specific DNA fragments belonging to S. Pullorum, this study used suppression subtractive hybridization. As such, the genome of the S. Pullorum C79-13 strain was subtracted from the genome of Salmonella enterica serovar Gallinarum 9 and Salmonella enterica serovar Enteritidis CMCC(B) 50041, respe...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Qiuchun L, Xu Y, Jiao X Tags: J Microbiol Biotechnol Source Type: journals

In situ single cell monitoring by isocyanide-functionalized ag and au nanoprobe-based Raman spectroscopy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The development of effective cellular imaging requires a specific labeling method for targeting, tracking, and monitoring cellular/molecular events in the living organism. For this purpose, we studied the cellular uptake of isocyanidefunctionalized silver and gold nanoparticles by surfaceenhanced Raman scattering (SERS). Inside a single mammalian cell, we could monitor the intracellular behavior of such nanoparticles by measuring the SERS spectra. The NC stretching band appeared clearly at ~2,100 cm-1 in the well-isolated spectral region from many organic constituents between 300 and 1,700 or 2,800 and 3,600 cm-1. The ...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Lee SY, Jang SH, Cho MH, Kim YM, Cho KC, Ryu PD, Gong MS, Joo SW Tags: J Microbiol Biotechnol Source Type: journals

Monitoring of cleavage preference for caspase-3 using recombinant protein substrates.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The apoptotic caspases have been classified in accordance with their substrate specificities, as the optimal tetrapeptide recognition motifs for a variety of caspases have been determined via positional scanning substrate combinatorial library technology. Here, we focused on two proteolytic recognition motifs, DEVD and IETD, owing to their extensive use in cell death assay. Although DEVE and IETD have been generally considered to be selective for caspase-3 and -8, respectively, the proteolytic cleavage of these substrates does not display absolute specificity for a particular caspase. Thus, we attempted to monitor the ...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Park K, Yi SY, Kim UL, Lee CS, Chung JW, Chung SJ, Kim M Tags: J Microbiol Biotechnol Source Type: journals

Carotenogenesis in Haematococcus lacustris: Role of Protein Tyrosine Phosphatases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study, we examined the inhibitory effects of protein tyrosine phosphatase (PTPase) inhibitors, including sodium orthovanadate (SOV), ammonium molybdate (AM), and iodoacetamide (IA), on cell growth, accumulation of astaxanthin, and PTPase activity in the photosynthetic algae Haematococcus lacustris. PTPase activity was assayed spectrophotometrically and was found to be inhibited 60% to 90% after treatment with the inhibitors. SOV markedly abolished PTPase activity, significantly activating the accumulation of astaxanthin. These data suggest that the accumulation of astaxanthin in H. lacustris results from...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Park JK, Tran PN, Kim JD, Hong SJ, Lee CG Tags: J Microbiol Biotechnol Source Type: journals

Studies on microbial transformation of meloxicam by fungi.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Screening-scale studies were performed with 26 fungal cultures for their ability to transform the anti-inflammatory drug meloxicam. Among the different fungi screened, a filamentous fungus, Cunninghamella blakesleeana NCIM 687, transformed meloxicam to three metabolites in significant quantities. The transformation of meloxicam was confirmed by high-performance liquid chromatography (HPLC). Based on the liquid chromatography-tandem mass spectrometry (LC-MS/MS) data, two metabolites were predicted to be 5-hydroxymethyl meloxicam and 5-carboxy meloxicam, the major mammalian metabolites reported previously. A new metaboli...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: G SP, Girisham S, Reddy SM Tags: J Microbiol Biotechnol Source Type: journals

Inactivation of the Wall-Associated De-N-acetylase (PgdA) of Listeria monocytogenes Results in Greater Susceptibility of the Cells to Induced Autolysis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Several species of Gram-positive bacteria have cell wall peptidoglycan (syn. murein) in which not all of the sugar moieties are N-acetylated. This has recently been shown to be a secondary effect, caused by the action of a peptidoglycan N-acetylglucosamine deacetylase. We have found that the opportunistic pathogen Listeria monocytogenes is unusual in having three enzymes with such activity, two of which remain in the cytoplasm. Here, we examine the enzyme (PgdA) that crosses the cytoplasmic membrane and is localized in the cell wall. We purified a hexa-His-tagged form of PgdA to study its activity and constructed a mut...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Popowska M, Kusio M, Szymanska P, Markiewicz Z Tags: J Microbiol Biotechnol Source Type: journals

Atromentin-induced apoptosis in human leukemia U937 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the course of screening for apoptotic substances that induce apoptosis in human leukemia U937 cells, a fungal strain, F000487, which exhibits potent inducible activity, was selected. The active compound was purified from an ethyl acetate extract of the microorganism by Sep-pak C18 column chromatography and HPLC, and was identified as atromentin by spectroscopic methods. This compound induced caspase-3 processing in human leukemia U937 cells. The caspase-3 and poly (ADP-ribose) polymerase (PARP) were induced by atromentin in a dose-dependent manner. Furthermore, DNA fragmentation was also induced by this compound in ...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Kim JH, Lee CH Tags: J Microbiol Biotechnol Source Type: journals

Study of Macrophage Activation and Structural Characteristics of Purified Polysaccharides from the Fruiting Body of Hericium erinaceus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the crude water-soluble polysaccharide HEF-P, which was obtained from the fruiting body of Hericium erinaceus by hot water extraction and ethanol precipitation, was fractionated by DEAEcellulose and Sepharose CL-6B column chromatographies. This process resulted in four polysaccharide fractions, named HEF-NP Fr I, HEF-NP Fr II, HEF-AP Fr I, and HEF-AP Fr II. Of these fractions, HEF-AP Fr II was able to upregulate the functional events mediated by activated macrophages, such as production of nitric oxide and expression of cytokines (IL-1¥â and TNF-¥á). The molecular mass of...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Lee JS, Min KM, Cho JY, Hong EK Tags: J Microbiol Biotechnol Source Type: journals

Seed-Conjugated Polymer Bead for ¥â2-Microglobulin Removal at Neutral pH.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Seed-Conjugated Polymer Bead for ¥â2-Microglobulin Removal at Neutral pH. J Microbiol Biotechnol. 2009 Sep;19(9):960-965 Authors: Kim M, Kang S, Myung EK, Ahn M, Choi JH, Paik SR, Lee YS beta2-Microglobulin (beta2m) is known to be a major factor for dialysis-related amyloidosis. We have studied beta2m removal through an aggregation process, which was initiated by a ligand that could catch the beta2m monomer and promote its aggregation into fibril. As a ligand, we have prepared beta2m fibril fragments and used them as a seed. The seed was coupled to PEGylated-PS beads to remove the monomeric &a...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Kim M, Kang S, Myung EK, Ahn M, Choi JH, Paik SR, Lee YS Tags: J Microbiol Biotechnol Source Type: journals

Optimization of the Functional Expression of Coprinus cinereus Peroxidase in Pichia pastoris by Varying the Host and Promoter.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the functional expression of this peroxidase was successfully achieved in the methylotrophic yeast Pichia pastoris. The expression level of CiP was increased by varying the microbial hosts and the expression promoters. Since a signal sequence, such as the alpha mating factor of Saccharomyces cerevisiae, was placed preceding the cDNA of the CiP coding gene, expressed recombinant CiP (rCiP) was secreted into the culture broth. The Mut+ Pichia pastoris host showed a 3-fold higher peroxidase activity, as well as 2-fold higher growth rate, compared with the Muts Pichia pastoris host. Furthermore, the AOX1 promote...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Kim SJ, Lee JA, Kim YH, Song BK Tags: J Microbiol Biotechnol Source Type: journals

Application of a Fed-Batch Bioprocess for the Heterologous Production of hSCOMT in Escherichia coli.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this paper, a fed-batch cultivation process in recombinant Escherichia coli BL21(DE3) bacteria, for the production of human soluble catechol-O-methyltransferase (hSCOMT), is presented. For the first time, a straightforward model is applied in a recombinant hSCOMT expression system and distinguishes an initial cell growth phase from a protein production phase upon induction. Specifically, the kinetic model predicts biomass, substrate, and product concentrations in the culture over time and was identified from a series of fed-batch experiments designed by testing several feed profiles. The main advantage of this model...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: L A P, Bonifacio MJ, Queiroz JA Tags: J Microbiol Biotechnol Source Type: journals

Evaluation of Optimal Culture Conditions for Recombinant Ghost Bacteria Vaccine Production with the Antigen of Streptococcus iniae GAPDH.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
For the production of ghost bacteria vaccine to prevent the streptococcal disease in aquaculture fish species, a double cassettes vector was constructed and cloned in Escherichia coli DH5alpha. Ghost bacteria vaccine production from Escherichia coli DH5alpha/pHCE-InaN-GAPDH-Ghost 37 SDM (SIG) was maximized at a glucose concentration of 1 g/l, agitation of 300 rpm, and aeration of 1 vvm. The maximal efficiency of ghost bacteria formation was obtained at the mid-exponential phase (OD600=2.0) with the concentration of 0.77 g/l for SIG. The molecular mass of GAPDH was detected at 67 kDa with the insoluble fraction, by SDS-...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Ra CH, Kim YJ, Park SJ, Jeong CW, Nam YK, Kim KH, Kim SK Tags: J Microbiol Biotechnol Source Type: journals

A Novel Tannase from the Xerophilic Fungus Aspergillus niger GH1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Aspergillus niger GH1 previously isolated and identified by our group as a wild tannase producer was grown under solid-state (SSC) and submerged culture (SmC) conditions to select the enzyme production system. For tannase purification, extracellular tannase was produced under SSC using polyurethane foam as the inert support. Tannase was purified to apparent homogeneity by ultrafiltration, anion-exchange chromatography, and gel filtration that led to a purified enzyme with a specific activity of 238.14 IU/mg protein with a final yield of 0.3% and a purification fold of 46. Three bands were found on the SDS-PAG with mole...
Source: Journal of Microbiology and Biotechnology - August 31, 2009 Category: Biotechnology Authors: Mata-Gomez M, Rodriguez LV, Ramos EL, Renovato J, Cruz-Hernandez MA, Rodriguez R, Contreras J, Aguilar CN Tags: J Microbiol Biotechnol Source Type: journals