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Contents
J Mol Microbiol Biotechnol 2019;29:I –IV (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - July 28, 2020 Category: Microbiology Source Type: research

Global Response of Phaeobacter inhibens DSM 17395 to Deletion of Its 262-kb Chromid Encoding Antibiotic Synthesis
The marine alphaproteobacteriumPhaeobacter inhibens DSM 17395, a member of theRoseobacter group, was recently shown to markedly enhance growth upon deletion of its 262-kb chromid encoding biosynthesis of tropodithietic acid (TDA). To scrutinize the metabolic/regulatory adaptations that underlie enhanced growth of the Δ262 mutant, its transcriptome and proteome compared to the wild type were investigated in process-controlled bioreactors with Casamino Acids as growth substrate. Genome resequencing revealed only few additional genetic changes (a heterogenic insertion, prophage activation, and several point mutati ons) betwe...
Source: Journal of Molecular Microbiology and Biotechnology - July 24, 2020 Category: Microbiology Source Type: research

Immunogenicity Evaluation of Chimeric Subunit Vaccine Comprising Adhesion Coli Surface Antigens from Enterotoxigenic Escherichia coli 
EnterotoxigenicEscherichia coli (ETEC) is the most common agent of diarrhea morbidity in developing countries. ETEC adheres to host intestinal epithelial cells via various colonization factors. The CooD and CotD proteins play a significant role in bacteria binding to the intestinal epithelial cells as adhesin tip subunits of CS1 and CS2 pili. The purpose here was to design a new construction containingcooD andcotD genes and use several types of bioinformatics software to predict the structural and immunological properties of the designed antigen. The fusion gene was synthesized with codon bias ofE. coli in order to increas...
Source: Journal of Molecular Microbiology and Biotechnology - July 9, 2020 Category: Microbiology Source Type: research

Study of the Enzymatic Capacity of Kluyveromyces marxianus for the Synthesis of Esters
Recently, biotechnological opportunities have been found in non-Saccharomyces yeasts because they possess metabolic characteristics that lead to the production of compounds of interest. It has been observed thatKluyveromyces marxianus has a great potential in the production of esters, which are aromatic compounds of industrial importance. The genetic bases that govern the synthesis of esters include a large group of enzymes, among which the most important are alcohol acetyl transferases (AATases) and esterases (AEATases), and it is known that some are present inK. marxianus, because it has genetic characteristics likeS. ce...
Source: Journal of Molecular Microbiology and Biotechnology - April 23, 2020 Category: Microbiology Source Type: research

Vaccination of Mice with Listeria ivanovii Expressing the Truncated M Protein of Porcine Reproductive and Respiratory Syndrome Virus Induces both Antigen-Specific CD4+ and CD8+ T Cell-Mediated Immunity
In this study, a part of the PRRSVORF6 gene (from 253 to 519 bp) encoding the hydrophilic domain of PRRSV M protein was integrated into twoListeria strains via homologous recombination to generate two PRRS vaccine candidates, namely LI-M ’ and LM-ΔactAplcB-M ’. Both candidate vaccines showed similar growth rate as their parent strains in culture media, but presented different bacterial loads in target organs. As the integrated heterogenous gene was not expressed, LM-ΔactAplcB-M ’ was excluded from the immunological test. In a mouse model, LI-M’ provoked both CD4+ and CD8+ T cell-mediated immunity. In addition, LI...
Source: Journal of Molecular Microbiology and Biotechnology - April 14, 2020 Category: Microbiology Source Type: research

Production of Mycobacterium bovis Antigens Included in Recombinant Occlusion Bodies of Baculovirus
Bovine tuberculosis (bTB) is a disease produced byMycobacterium bovis that affects livestock, wild animals, and humans. The classical diagnostic method to detect bTB is measuring the response induced with the intradermal injection of purified protein derivative ofM. bovis (PPDb). Another ancillary bTB test detects IFN- γ produced in whole blood upon stimulation with PPDb, protein/peptide cocktails, or individual antigens. Among the most usedM. bovis antigens in IFN- γ assays are the secreted proteins ESAT-6 and CFP-10, which together with antigen Rv3615c improve the sensitivity of the test in comparison to PPDb. Protein ...
Source: Journal of Molecular Microbiology and Biotechnology - April 7, 2020 Category: Microbiology Source Type: research

Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp. H11
This study aimed to improveEnteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system ofVibrio sp. H11, an EP-utilizing microbial strain. Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP. To maximize the capability of strain H11 to hydrolyze EP, both the culture medium and the composition were optimized. Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation. When 50 g/L of EP were treated with crude H11 enzyme, the concen...
Source: Journal of Molecular Microbiology and Biotechnology - March 6, 2020 Category: Microbiology Source Type: research

Non-Caloric Artificial Sweeteners Modulate the Expression of Key Metabolic Genes in the Omnipresent Gut Microbe Escherichia coli 
The human gut is inhabited by several hundred different bacterial species. These bacteria are closely associated with our health and well-being. The composition of these diverse commensals is influenced by our dietary intakes. Non-caloric artificial sweeteners (NAS) have gained global popularity, particularly among diabetic patients, due to their perceived health benefits, such as reduction of body weight and maintenance of blood glucose level compared to caloric sugars. Recent studies have reported that these artificial sweeteners can alter the composition of gut microbiota and, thus, affect our normal physiological state...
Source: Journal of Molecular Microbiology and Biotechnology - December 18, 2019 Category: Microbiology Source Type: research

Characterization of Alanine Dehydrogenase and Its Effect on Streptomyces coelicolor A3(2) Development in Liquid Culture
Streptomyces, the most important group of industrial microorganisms, is harvested in liquid cultures for the production of two-thirds of all clinically relevant secondary metabolites. It is demonstrated here that the growth ofStreptomyces coelicolor A3(2) is impacted by the deletion of the alanine dehydrogenase (ALD), an essential enzyme that plays a central role in the carbon and nitrogen metabolism. A long lag-phase growth followed by a slow exponential growth ofS. coelicolor due to ALD gene deletion was observed in liquid yeast extract mineral salt culture. The slow lag-phase growth was replaced by the normal wild-type ...
Source: Journal of Molecular Microbiology and Biotechnology - December 18, 2019 Category: Microbiology Source Type: research

The Life Cycle of Dictyostelium discoideum Is Accelerated via MAP Kinase Cascade by a Culture Extract Produced by a Synthetic Microbial Consortium
A cellular slime mold,Dictyostelium discoideum, is an amoeboid organism that has a unique life cycle consisting of distinctly separated vegetative and developmental phases. Thus, this organism presents a rare opportunity in which to examine the effects of bioactive substances on separate cellular activities. In this research, we investigated the effect of a culture extract, termed EMXG, produced by a synthetic microbial consortium. EMXG promoted proliferative response of amoeba cells. It further accelerated the developmental phase, leading to the preferred fruiting body formation from fewer cells. Furthermore, EMXG modulat...
Source: Journal of Molecular Microbiology and Biotechnology - December 5, 2019 Category: Microbiology Source Type: research

A Riboflavin Transporter in Bdellovibrio exovorous JSS
The ImpX transporters of the drug/metabolite transporter superfamily were first proposed to transport riboflavin (RF; vitamin B2) based on findings of acis-regulatory RNA element responding to flavin mononucleotide (an FMN riboswitch).Bdellovibrio exovorous JSS has a homolog belonging to this superfamily. It has 10 TMSs and shows 30% identity to the previously characterized ImpX transporter fromFusobacterium nucleatum. However, the ImpX homolog is not regulated by an FMN-riboswitch. In order to test the putative function of the ImpX homolog fromB. exovorous (BexImpX), we cloned and heterologously expressed its gene. We use...
Source: Journal of Molecular Microbiology and Biotechnology - September 11, 2019 Category: Microbiology Source Type: research

Contents
J Mol Microbiol Biotechnol 2018;28:I –IV (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - July 18, 2019 Category: Microbiology Source Type: research

Studies of the Listeria monocytogenes Cellobiose Transport Components and Their Impact on Virulence Gene Repression
Conclusion: The two EIIA/BCel pairs are similarly efficient as phosphoryl donors in EIICCel1-catalyzed cellobiose transport and CelR regulation. The permanent virulence gene repression in thecelA double mutant further supports a role of PTSCel components in PrfA regulation.J Mol Microbiol Biotechnol (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - July 3, 2019 Category: Microbiology Source Type: research

Comparison of DNA Extraction Efficiency and Reproducibility of Different Aeration Diffuser Biofilms Using Bead-Beating Protocol
An existing bead-beating DNA extraction protocol was employed to compare the DNA extraction recovery and fragment quality of 6 different aeration diffuser biofilms.Escherichia coli,Gordonia amarae, and mixed liquor were used as controls. The fraction of total DNAbiofilm decreased monotonically with increasing number of beat beatings (BB) when the amount of DNA present was sufficient (#x3e;4 μgDNA/cm2), excluding the ceramic disk. While controls required only 2 BBs, 3 out of 5 BBs achieved ≥70% of total DNA (70.3 ± 1.7%) for 5 out of 6 biofilms. Quantitative polymerase chain reaction (PCR) analyses of 353 and 1,505 base...
Source: Journal of Molecular Microbiology and Biotechnology - June 24, 2019 Category: Microbiology Source Type: research

Ubiquitous Promoters Direct the Expression of Fatty Acid Delta-6 Desaturase from Nile Tilapia ( Oreochromis niloticus ) in Saccharomyces cerevisia e
In general, promoters have significant influence on recombinant protein production. Herein, we compared the performance of actin (pACT), phosphoglycerate kinase (pPGK), and translational elongation factor (pTEF) promoters for driving the expression of fatty acid delta-6 ( Δ6) desaturase from Nile tilapia (Oreochromis niloticus;Oni-fads2) inSaccharomyces cerevisiae. Our results showed that by applying real-time RT-PCR, the highest level ofOni-fads2 mRNA was observed inS. cerevisiae carrying the expression vector driven by pTEF promoters. Exogenous substrate C18:2n-6 was used to determine Δ6 activity by quantitatively dete...
Source: Journal of Molecular Microbiology and Biotechnology - June 24, 2019 Category: Microbiology Source Type: research