Journal of Molecular and Cellular CardiologyThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - November 6, 2009 Category: Cytology Source Type: journals

ISHR 2010 Congress Awards - Call for NominationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - October 12, 2009 Category: Cytology Tags: Announcement: ISHR 2010 Congress - Call for Nominations Source Type: journals

Erratum to “Oxidative stress and cardiovascular disease: novel tools give (free) radical insight” [J. Mol. Cell. Cardiol. 47 (2009) 372–381]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The publisher regrets that a production error resulted in the omission of the following conflict of interest statement. Ian Fearon has been employed within British American Tobacco Group R&D since March 2008 and is currently a stockholder in the company. Stephen Faux has been employed at Applied Technologies (Cambridge) Limited since June 2007 and within British American Tobacco Group R&D since April 2009, and is currently a stockholder in the company. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - October 12, 2009 Category: Cytology Authors: Ian M. Fearon, Stephen P. Faux Tags: Erratum Source Type: journals

EditorialEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It is a pleasure to congratulate Dr. Richard Bing on the occasion of his 100th birthday. A summary of his life and scientific contributions appears in this issue . It is fitting that this article appears in the Journal of Molecular and Cellular Cardiology as Dr. Bing (together with Lionel Opie) founded the journal in 1970 and was its first Editor in Chief. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - October 12, 2009 Category: Cytology Authors: Heinrich Taegtmeyer Tags: Editorials Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - October 12, 2009 Category: Cytology Source Type: journals

Nitric oxide inhibits endothelin-1-induced neonatal cardiomyocyte hypertrophy via a RhoA-ROCK-dependent pathwayEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Although nitric oxide (NO) has received extensive attention as an anti-hypertrophic agent the mechanisms underlying its regulation of endothelin-1 (ET-1) have not been fully elucidated. Since RhoA has been identified as an important mediator of cardiac hypertrophy and is inhibited by NO in vascular tissue, we sought to determine whether the anti-ET-1 effects of NO in cardiomyocytes were mediated via inhibition of the RhoA-ROCK cascade in the context of cardiac hypertrophy. Neonatal rat ventricular myocytes were cultured in the presence of ET-1 (10 nM) with or without pre-treatment with the NO donor S-nitroso-n-a...
Source: Journal of Molecular and Cellular Cardiology - October 5, 2009 Category: Cytology Authors: J. Craig Hunter, Asad Zeidan, Sabzali Javadov, Ana Kilić, Venkatesh Rajapurohitam, Morris Karmazyn Tags: Regular Articles Source Type: journals

Dok-5 is involved in cardiomyocyte differentiation through PKB/FOXO3a pathwayEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The insulin receptor substrate (IRS) family plays important roles in cellular growth, signaling, and survival in the brain. We identified IRS6/Dok-5, a member of the IRS family, also expressed in heart. Dok-5 expression level significantly increased during cardiomyocyte differentiation of P19CL6 cells. To understand the mechanism of Dok-5 gene expression and regulation during cardiomyocyte differentiation, we first mapped the transcription start site of the mouse Dok-5 gene and characterized its promoter regions. Truncation and mutation analysis of the Dok-5 promoter identified the forkhead binding element respon...
Source: Journal of Molecular and Cellular Cardiology - October 5, 2009 Category: Cytology Authors: Jianyan Wen, Qing Xia, Cheng Wang, Wei Liu, Yang Chen, Jing Gao, Yanhua Gong, Bin Yin, Yuannan Ke, Boqin Qiang, Jiangang Yuan, Xiaozhong Peng Tags: Regular Articles Source Type: journals

Dihydrofolate reductase and biopterin recycling in cardiovascular diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nitric oxide, generated by the nitric oxide synthase (NOS) enzymes, plays pivotal roles in cardiovascular homeostasis and in the pathogenesis of cardiovascular disease. The NOS cofactor, tetrahydrobiopterin (BH4), is an important regulator of NOS function, since BH4 is required to maintain enzymatic coupling of l-arginine oxidation, to produce NO. Loss or reduction of BH4 is associated with NOS uncoupling, resulting in production of superoxide rather than NO. Electron paramagnetic resonance spectroscopy studies have shown that BH4 both stabilizes and donates electrons to the ferrous-dioxygen complex in the oxygenase domain...
Source: Journal of Molecular and Cellular Cardiology - October 5, 2009 Category: Cytology Authors: Mark J. Crabtree, Keith M. Channon Tags: Editorial Source Type: journals

Carbamylated darbepoetin derivative prevents endothelial progenitor cell damage with no effect on angiogenesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study evaluates whether carbamylated erythropoietin is as effective as recombinant human erythropoietin in protecting endothelial progenitor cells (EPCs) from apoptosis without stimulating erythropoiesis. Experiments were performed in an erythroid cell line (UT-7) and in human EPCs. Cell signals regulating proliferation and apoptosis (Jak-2, Akt, Erk1/2, NFκB and Stat-5) were measured by Western blotting. In human EPCs, cell senescence, apoptosis and proliferation were assessed by acidic β-gal and measurement of telomere length, TUNEL and PCNA labeling, respectively. Angiogenesis was evaluated using the endothelial ...
Source: Journal of Molecular and Cellular Cardiology - September 25, 2009 Category: Cytology Authors: Rafael Ramirez, Julia Carracedo, Sonia Nogueras, Paula Buendia, Ana Merino, Sagrario Cañadillas, Mariano Rodríguez, Ciro Tetta, Alejandro Martin-Malo, Pedro Aljama Tags: Regular Articles Source Type: journals

Angiotensin II type 1 receptor-associated protein prevents vascular smooth muscle cell senescence via inactivation of calcineurin/nuclear factor of activated T cells pathwayEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the possibility that ATRAP could attenuate AT1 receptor-mediated vascular senescence via inactivation with the calcineurin/NFAT pathway. Ang II stimulation significantly increased senescence-associated β-galactosidase (SA-β-gal)-stained cells, oxidative stress, and expression of p53 and p21 in wild-type (WT) vascular smooth muscle cells (VSMC). Moreover, in WT VSMC, Ang II stimulation enhanced NFAT transcriptional activity, which was prevented by CAML-siRNA treatment. NFAT-siRNA treatment attenuated Ang-II-increased SA-β-gal activity and p53 and p21 expression. Treatment with a calcineurin activity inhibitor...
Source: Journal of Molecular and Cellular Cardiology - September 22, 2009 Category: Cytology Authors: Li-Juan Min, Masaki Mogi, Kouichi Tamura, Jun Iwanami, Akiko Sakata, Teppei Fujita, Kana Tsukuda, Fei Jing, Masaru Iwai, Masatsugu Horiuchi Tags: Regular Articles Source Type: journals

Adverse ventricular remodeling and exacerbated NOS uncoupling from pressure-overload in mice lacking the β3-adrenoreceptorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we tested whether lack of β3-AR influences the myocardial response to pressure-overload. Baseline echocardiography in mice lacking β3-AR (β3−/−) compared to wild type (WT) showed mild LV hypertrophy at 8 weeks that worsened as they aged. β3−/− mice had much greater mortality after transverse aortic constriction (TAC) than WT controls. By 3 weeks of TAC, systolic function was worse. After 9 weeks of TAC, β3−/− mice also had greater LV dilation, myocyte hypertrophy and enhanced fibrosis. NOS activity declined in β3−/−TAC hearts after 9 weeks, and total and NOS-dependent superoxide ros...
Source: Journal of Molecular and Cellular Cardiology - September 21, 2009 Category: Cytology Authors: An L. Moens, Jordan S. Leyton-Mange, Xiaolin Niu, Ronghua Yang, Oscar Cingolani, Elisabeth K. Arkenbout, Hunter C. Champion, Djahida Bedja, Kathleen L. Gabrielson, Juan Chen, Yong Xia, Ashley B. Hale, Keith M. Channon, Marc K. Halushka, Norman Barker, Flo Tags: Regular Articles Source Type: journals

Preservation of mitochondrial function with cardiopulmonary resuscitation in prolonged cardiac arrest in ratsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: During cardiac arrest (CA), myocardial perfusion is solely dependent on cardiopulmonary resuscitation (CPR) although closed-chest compressions only provide about 10–20% of normal myocardial perfusion. The study was conducted in a whole animal CPR model to determine whether CPR-generated oxygen delivery preserves or worsens mitochondrial function. Male Sprague-Dawley rats (400–450 g) were randomly divided into four groups: (1) BL (instrumentation only, no cardiac arrest), (2) CA15 (15 min cardiac arrest without CPR), (3) CA25 (25 min cardiac arrest without CPR) and (4) CPR (15 min cardiac arrest, followed ...
Source: Journal of Molecular and Cellular Cardiology - September 14, 2009 Category: Cytology Authors: Steve T. Yeh, Hsin-Ling Lee, Sverre E. Aune, Chwen-Lih Chen, Yeong-Renn Chen, Mark G. Angelos Tags: Regular Articles Source Type: journals

Interaction of myocardial insulin receptor and IGF receptor signaling in exercise-induced cardiac hypertrophyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Insulin-like growth factor-1 (IGF-1) signaling has recently been implicated in the development of cardiac hypertrophy after long-term endurance training, via mechanisms that may involve energetic stress. Given the potential overlap of insulin and IGF-1 signaling we sought to determine if both signaling pathways could contribute to exercise-induced cardiac hypertrophy following shorter-term exercise training. Studies were performed in mice with cardiac-specific IGF-1 receptor (IGF1R) knockout (CIGFRKO), mice with cardiac-specific insulin receptor (IR) knockout (CIRKO), CIGFRKO mice that lacked one IR allele in car...
Source: Journal of Molecular and Cellular Cardiology - September 8, 2009 Category: Cytology Authors: Hiroyuki Ikeda, Ichiro Shiojima, Yukako Ozasa, Masashi Yoshida, Martin Holzenberger, C. Ronald Kahn, Kenneth Walsh, Takashi Igarashi, E. Dale Abel, Issei Komuro Tags: Regular Articles Source Type: journals

SERCA1 expression enhances the metabolic efficiency of improved contractility in post-ischemic heartEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we determine whether the metabolic coupling to function improves with SERCA treatment. PBS (control) or adenovirus carrying the cDNA for SERCA1 was delivered via coronary perfusion in vivo to Sprague-Dawley rat hearts. Three days following gene transfer, isolated hearts were perfused with 0.4 mM [2,4,6,8,10,12,14,16-13C8] palmitate and 5 mM glucose, and subjected to 15-min ischemia followed by 40-min reperfusion. Consistent with myocardial stunning, rate pressure product (RPP) and left ventricular developed pressure (LVDP) were depressed 30–40% (p (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - September 8, 2009 Category: Cytology Authors: J. Michael O'Donnell, Kayla Pound, Xianyao Xu, E. Douglas Lewandowski Tags: Regular Articles Source Type: journals

Involvement of Src in L-type Ca2+ channel depression induced by macrophage migration inhibitory factor in atrial myocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine that controls inflammatory processes, and inflammation is known to play an important role in the pathogenesis of atrial fibrillation (AF). The present study sought to investigate whether MIF expression is responsible for the changes in L-type Ca2+ currents (ICa,L) seen in AF. Whole-cell voltage-clamp recordings and biochemical assays were used to study the regulation and expression of ICa,L in human atrial myocytes and in HL-1 cells. Basal ICa,L was reduced in AF compared to sinus rhythm (SR) controls, mRNA and protein levels of the pore-formi...
Source: Journal of Molecular and Cellular Cardiology - September 8, 2009 Category: Cytology Authors: Fang Rao, Chun-Yu Deng, Shu-Lin Wu, Ding-Zhang Xiao, Xi-Yong Yu, Su-Juan Kuang, Qiu-Xiong Lin, Zhi-Xin Shan Tags: Regular Articles Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - September 3, 2009 Category: Cytology Source Type: journals

Krüppel-like factor 4 mediates histone deacetylase inhibitor-induced prevention of cardiac hypertrophyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Recently, we reported that histone deacetylase (HDAC) inhibitors block cardiac hypertrophy and that activation of HDAC2, one of the class I HDACs, is required for hypertrophy. In the present study, we tried to find the downstream target of HDAC inhibitor by utilizing cardiomyocytes and H9c2 cells. Both trichostatin A (TSA, class I and II HDAC inhibitor) and SK7041 (SK, class I HDAC blocker) attenuated the expression level and promoter activity of Nppa (natriuretic polypeptide precursor type A) and Myh7 (myosin heavy polypeptide 7), which are fetal genes associated with hypertrophy. Promoter-mapping revealed that ...
Source: Journal of Molecular and Cellular Cardiology - September 2, 2009 Category: Cytology Authors: Hae Jin Kee, Hyun Kook Tags: Regular Articles Source Type: journals

Selective activation of the “b” splice variant of phospholipase Cβ1 in chronically dilated human and mouse atriaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined PLC activation in right and left atrial appendage from patients with mitral valve disease (VHD) and in a mouse model of dilated cardiomyopathy caused by transgenic overexpression of the stress-activated protein kinase, mammalian sterile 20 like kinase 1 (Mst1) (Mst1-TG). PLC activation was heightened 6- to 10-fold in atria from VHD patients compared with right atrial tissue from patients undergoing coronary artery bypass surgery (CABG) and was also heightened in the dilated atria from Mst1-TG. PLC activation in human left atrial appendage and in mouse left atria correlated with left atrial size, implying a rela...
Source: Journal of Molecular and Cellular Cardiology - September 1, 2009 Category: Cytology Authors: Elizabeth A. Woodcock, David R. Grubb, Theresa M. Filtz, Silvana Marasco, Jieting Luo, Tiffany J. McLeod-Dryden, David M. Kaye, Junichi Sadoshima, Xiao-Jun Du, Chiew Wong, Julie R. McMullen, Anthony M. Dart Tags: Regular Articles Source Type: journals

Beneficial effects of soluble epoxide hydrolase inhibitors in myocardial infarction model: Insight gained using metabolomic approachesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Myocardial infarction (MI) leading to myocardial cell loss represents one of the common causes leading to cardiac failure. We have previously demonstrated the beneficial effects of several potent soluble epoxide hydrolase (sEH) inhibitors in cardiac hypertrophy. sEH catalizes the conversion of epoxyeicosatrienoic acids (EETs) to form the corresponding dihydroxyeicosatrienoic acids (DHETs). EETs are products of cytochrome P450 epoxygenases that have vasodilatory properties. Additionally, EETs inhibit the activation of nuclear factor (NF)-κB-mediated gene transcription. Motivated by the potential to uncover a new ...
Source: Journal of Molecular and Cellular Cardiology - September 1, 2009 Category: Cytology Authors: Ning Li, Jun-Yan Liu, Valeriy Timofeyev, Hong Qiu, Sung Hee Hwang, Dipika Tuteja, Ling Lu, Jun Yang, Hideki Mochida, Reginald Low, Bruce D. Hammock, Nipavan Chiamvimonvat Tags: Regular Articles Source Type: journals

Pleiotropic effects of neutrophils on myocyte apoptosis and left ventricular remodeling during early volume overloadEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Most of the available evidence on the role of neutrophils on pathological cardiac remodeling has been pertained after acute myocardial infarction. However, whether neutrophils directly contribute to the pathogenesis of cardiac remodeling after events other than acute myocardial infarction remains unknown. Here we show that acute eccentric hypertrophy induced by aorto-caval fistula (ACF) in the rats induced an increase in the inflammatory response characterized by activation of the STAT pathway and increased infiltration of neutrophils in the myocardium. This early inflammation was associated with a decrease in in...
Source: Journal of Molecular and Cellular Cardiology - August 31, 2009 Category: Cytology Authors: Mikhail A. Kolpakov, Rachid Seqqat, Khadija Rafiq, Hang Xi, Kennneth B. Margulies, Joseph R. Libonati, Pamela Powel, Steven R. Houser, Louis J. Dell'italia, Abdelkarim Sabri Tags: Regular Articles Source Type: journals

Dietary ω-3 fatty acids alter cardiac mitochondrial phospholipid composition and delay Ca2+-induced permeability transitionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, dietary supplementation with EPA + DHA altered mitochondrial membrane phospholipid fatty acid composition in normal and infarcted hearts, but delayed MPTP opening only in normal hearts. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 25, 2009 Category: Cytology Authors: Karen M. O'Shea, Ramzi J. Khairallah, Genevieve C. Sparagna, Wenhong Xu, Peter A. Hecker, Isabelle Robillard-Frayne, Christine Des Rosiers, Tibor Kristian, Robert C. Murphy, Gary Fiskum, William C. Stanley Tags: Regular Articles Source Type: journals

Glucose-regulated protein 78 inhibits scavenger receptor A-mediated internalization of acetylated low density lipoproteinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Class A scavenger receptor (SR-A) plays an important role in foam cell formation. However, the mechanism underlying the internalization of the receptor–ligand complexes remains unclear. The aim of the present study was to investigate the molecular mechanism to regulate SR-A-mediated intracellular lipid accumulation in macrophages. A pull-down assay was performed and glucose-regulated protein 78 (GRP78) was identified to bind with the cytoplasmic domain of SR-A (CSR-A). Immunoprecipitation and artificially expressed protein binding assay demonstrated the direct specific binding of GRP78 with SR-A in cells. Indir...
Source: Journal of Molecular and Cellular Cardiology - August 20, 2009 Category: Cytology Authors: Jingjing Ben, Song Gao, Xudong Zhu, Yuan Zheng, Yan Zhuang, Hui Bai, Yong Xu, Yong Ji, Jiahao Sha, Zhigang He, Qi Chen Tags: Regular Articles Source Type: journals

Notch1 represses osteogenic pathways in aortic valve cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we examined the mechanism by which NOTCH1 represses aortic valve calcification. Heterozygous Notch1-null (Notch1+/-) mice had greater than fivefold more aortic valve calcification than age- and sex-matched wildtype littermates. Inhibition of Notch signaling in cultured sheep aortic valve interstitial cells (AVICs) also increased calcification more than fivefold and resulted in gene expression typical of osteoblasts. We found that Notch1 normally represses the gene encoding bone morphogenic protein 2 (Bmp2) in murine aortic valves in vivo and in aortic valve cells in vitro. siRNA-mediated knockdown of Bmp2 bl...
Source: Journal of Molecular and Cellular Cardiology - August 19, 2009 Category: Cytology Authors: Vishal Nigam, Deepak Srivastava Tags: Regular Articles Source Type: journals

Extracellular superoxide dismutase regulates cardiac function and fibrosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Extracellular superoxide dismutase (EC-SOD) is an antioxidant that protects the heart from ischemia and the lung from inflammation and fibrosis. The role of cardiac EC-SOD under normal conditions and injury remains unclear. Cardiac toxicity, a common side effect of doxorubicin, involves oxidative stress. We hypothesize that EC-SOD is critical for normal cardiac function and protects the heart from oxidant-induced fibrosis and loss of function. C57BL/6 and EC-SOD-null mice were treated with doxorubicin, 15 mg/kg (i.p.). After 15 days, echocardiography was used to assess cardiac function. Left ventricle (LV) tiss...
Source: Journal of Molecular and Cellular Cardiology - August 18, 2009 Category: Cytology Authors: Corrine R. Kliment, Hagir B. Suliman, Jacob M. Tobolewski, Crystal M. Reynolds, Brian J. Day, Xiaodong Zhu, Charles F. McTiernan, Kenneth R. McGaffin, Claude A. Piantadosi, Tim D. Oury Tags: Regular Articles Source Type: journals

Adenosine A2A and A2B receptors work in concert to induce a strong protection against reperfusion injury in rat heartsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, A2A and A2B receptors work in concert to prevent reperfusion injury in rat hearts treated with NECA. NECA may protect the heart by modulating the mPTP opening through inactivating mitochondrial GSK-3β. A simultaneous stimulation of A2A and A2B receptors at reperfusion is required to produce a strong cardioprotection against reperfusion injury. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 18, 2009 Category: Cytology Authors: Jinkun Xi, Rachel McIntosh, Xiangjun Shen, SungRyul Lee, Guillaume Chanoit, Hugh Criswell, David A. Zvara, Zhelong Xu Tags: Regular Articles Source Type: journals

Richard Bing at 100: Reflections on a lion in winterEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The upcoming 100th birthday of Dr. Richard J. Bing () provides a welcome opportunity to reflect on this extraordinary man, on the times he has lived in, and on the contributions he has made to investigative cardiology, spanning nearly seven decades of original work. In addition we celebrate in Dr. Bing as one of the founders of the International Study Group for Research in Cardiac Metabolism, later renamed International Society for Heart Research (ISHR) and a founding editor of this Journal. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 18, 2009 Category: Cytology Authors: Heinrich Taegtmeyer Tags: Editorials Source Type: journals

Cell therapy enhances function of remote non-infarcted myocardiumEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Cell transplantation improves cardiac function after myocardial infarction; however, the underlying mechanisms are not well-understood. Therefore, the goals of this study were to determine if neonatal rat cardiomyocytes transplanted into adult rat hearts 1 week after infarction would, after 8–10 weeks: 1) improve global myocardial function, 2) contract in a Ca2+ dependent manner, 3) influence mechanical properties of remote uninjured myocardium and 4) alter passive mechanical properties of infarct regions. The cardiomyocytes formed small grafts of ultrastructurally maturing myocardium that enhanced fractional s...
Source: Journal of Molecular and Cellular Cardiology - August 16, 2009 Category: Cytology Authors: Alicia Moreno-Gonzalez, F. Steven Korte, Jin Dai, Kent Chen, Bryan Ho, Hans Reinecke, Charles E. Murry, Michael Regnier Tags: Regular Articles Source Type: journals

Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in ratsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Adipose tissue-derived stem cells have been demonstrated to differentiate into cardiomyocytes and vascular endothelial cells. Here we investigate whether mature adipocyte-derived dedifferentiated fat (DFAT) cells can differentiate to cardiomyocytes in vitro and in vivo by establishing DFAT cell lines via ceiling culture of mature adipocytes. DFAT cells were obtained by dedifferentiation of mature adipocytes from GFP-transgenic rats. We evaluated the differentiating ability of DFAT cells into cardiomyocytes by detection of the cardiac phenotype markers in immunocytochemical and RT-PCR analyses in vitro. We also ex...
Source: Journal of Molecular and Cellular Cardiology - August 16, 2009 Category: Cytology Authors: Medet Jumabay, Taro Matsumoto, Shin-ichiro Yokoyama, Koichiro Kano, Yoshiaki Kusumi, Takayuki Masuko, Masako Mitsumata, Satoshi Saito, Atsushi Hirayama, Hideo Mugishima, Noboru Fukuda Tags: Regular Articles Source Type: journals

Dysfunction of ouabain-induced cardiac contractility in mice with heart-specific ablation of Na,K-ATPase β1-subunitEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our results demonstrate that Na,K-β1 plays an essential role in regulating cardiac contractility and that its loss is associated with significant pathophysiology of the heart. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 16, 2009 Category: Cytology Authors: Sonali P. Barwe, Maria C. Jordan, Anna Skay, Landon Inge, Sigrid A. Rajasekaran, Daniel Wolle, Christina L. Johnson, Patricia Neco, Kun Fang, Nora Rozengurt, Joshua I. Goldhaber, Kenneth P. Roos, Ayyappan K. Rajasekaran Tags: Regular Articles Source Type: journals

Activation of purinergic receptors by ATP induces ventricular tachycardia by membrane depolarization and modifications of Ca2+ homeostasisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, extracellular ATP activates purinergic receptors and induces arrhythmic activity through modifications of Ca2+ homeostasis and an activation of depolarizing membrane currents. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 11, 2009 Category: Cytology Authors: Iman S. Gurung, Asli Kalin, Andrew A. Grace, Christopher L.-H. Huang Tags: Regular Articles Source Type: journals

Mechanistic insights into folic acid-dependent vascular protection: Dihydrofolate reductase (DHFR)-mediated reduction in oxidant stress in endothelial cells and angiotensin II-infused mice: A novel HPLC-based fluorescent assay for DHFR activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Folate supplementation improves endothelial function in patients with hyperhomocysteinemia. Mechanistic insights into potential benefits of folate on vascular function in general population however, remain mysterious. Expression of dihydrofolate reductase (DHFR) was markedly increased by folic acid (FA, 50 μmol/L, 24 h) treatment in endothelial cells. Tetrahydrofolate (THF) is formed after incubation of purified DHFR or cellular extracts with 50 μmol/L of substrate dihydrofolic acid. THF could then be detected and quantified by high performance liquid chromatography (HPLC) with a fluorescent detector (295/36...
Source: Journal of Molecular and Cellular Cardiology - August 5, 2009 Category: Cytology Authors: Ling Gao, Karel Chalupsky, Enrico Stefani, Hua Cai Tags: Highlighted Article Source Type: journals

Chronic doxorubicin cardiotoxicity is mediated by oxidative DNA damage-ATM-p53-apoptosis pathway and attenuated by pitavastatin through the inhibition of Rac1 activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Doxorubicin is known to have cumulative dose-dependent cardiotoxicity, and a tumor suppressor protein p53 has been implicated in the pathogenesis of doxorubicin cardiotoxicity. However, how p53 is induced by doxorubicin and mediates the cardiotoxic effects of doxorubicin remains elusive. In cultured cardiac myocytes, doxorubicin induced oxidative stress, DNA damage, ATM activation, and p53 induction. A free radical scavenger NAC attenuated all of these events, whereas an ATM kinase inhibitor wortmannin attenuated doxorubicin-induced ATM activation and p53 induction but not oxidative stress. Doxorubicin treatment ...
Source: Journal of Molecular and Cellular Cardiology - August 4, 2009 Category: Cytology Authors: Masashi Yoshida, Ichiro Shiojima, Hiroyuki Ikeda, Issei Komuro Tags: Regular Articles Source Type: journals

Role of reactive oxygen species (ROS) in angiotensin II-induced stimulation of the cardiac Na+/HCO3− cotransportEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: The sarcolemmal Na+/HCO3− cotransporter (NBC) plays an important role in intracellular pH (pHi) regulation in the heart. In the present work we studied, in isolated cat ventricular myocytes, the role of Angiotensin II (Ang II) and reactive oxygen species (ROS) production as potential activators of the NBC. pHi was measured in single cells in a medium with HCO3− using the fluorescent pH indicator BCECF. The NH4+ pulse method was used to induce an intracellular acid load and the acid efflux (JH) in the presence of the Na+/H+ exchanger blocker HOE642 (10 μM) was calculated as indicator of NBC activity. The fol...
Source: Journal of Molecular and Cellular Cardiology - July 30, 2009 Category: Cytology Authors: Verónica C. De Giusti, Carolina D. Garciarena, Ernesto A. Aiello Tags: Regular Articles Source Type: journals

Serotonin transporter gene deficiency is associated with sudden death of newborn mice through activation of TGF-β1 signallingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, for the first time we obtained a SERT-knockout (KO) mouse model which reproduces SIDS phenotype. SERT-KO mice were generated by mating SERTCre/+ heterozygous mice. The SERT-KO mouse embryos at the pre-natal stage E18.5 were lacking of SERT mRNA and protein expression in the heart. A premature death of 75% of SERT-KO mice occurred in the first week after birth. LacZ staining of whole mounts and tissue sections of the heart from SERTCre/+;ROSA26R adult mice and E18.5 embryos demonstrated a marked localized expression of SERT in the right ventricle, the conal region, the vasculature, the atrial septum, the vent...
Source: Journal of Molecular and Cellular Cardiology - July 30, 2009 Category: Cytology Authors: Luigi Michele Pavone, Anna Spina, Silviana Rea, Dionea Santoro, Vincenzo Mastellone, Pietro Lombardi, Luigi Avallone Tags: Regular Articles Source Type: journals

Fellowship of the ISHREmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 28, 2009 Category: Cytology Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 28, 2009 Category: Cytology Source Type: journals

Does small-conductance calcium-activated potassium channel contribute to cardiac repolarization?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Small-conductance calcium-activated potassium channels (SK channels) have a significant role in neurons. Since they directly integrate calcium handling with repolarization, in heart their role would be particularly important. However, their contribution to cardiac repolarization is still unclear. A previous study reported a significant lengthening effect of apamin, a selective SK channel inhibitor, on the action potential duration in atrial and ventricular mouse cardiomyocytes and human atrial cells. They concluded that these channels provide an important functional link between intracellular calcium handling and...
Source: Journal of Molecular and Cellular Cardiology - July 26, 2009 Category: Cytology Authors: Norbert Nagy, Viktória Szűts, Zoltán Horváth, György Seprényi, Attila S. Farkas, Károly Acsai, János Prorok, Miklós Bitay, Attila Kun, János Pataricza, Julius Gy. Papp, Péter P. Nánási, András Varró, András Tóth Tags: Regular Articles Source Type: journals

Threonine-5 at the N-terminus can modulate sarcolipin function in cardiac myocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Sarcolipin (SLN) has emerged as an important regulator of the atrial sarcoplasmic reticulum (SR) Ca2+ transport. The inhibitory effect of SLN on cardiac SR Ca2+ ATPase (SERCA) pump can be relieved by β-adrenergic stimulation, which indicates that SLN is a reversible inhibitor. However, the mechanism of this reversible regulation of SERCA pump by SLN is yet to be determined. In the current study using adult rat ventricular myocytes we provide evidence that the threonine 5 (T5) residue at the N-terminus of SLN which is conserved among various species, critically regulates the SLN function. Point mutation of T5→a...
Source: Journal of Molecular and Cellular Cardiology - July 23, 2009 Category: Cytology Authors: Poornima Bhupathy, Gopal J. Babu, Makoto Ito, Muthu Periasamy Tags: Regular Articles Source Type: journals

In MemoriamEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Howard E. Morgan, one of the leading experimental cardiologists of the 20th century, died after a brief illness on March 2, 2009 in Estero, Florida. He died of complications of a fall which occurred while he was receiving coumadin therapy for atrial fibrillation. Howard is survived by his wife Donna and a daughter Patricia Morgan Wehler of East Berlin Pa. as well as two grandsons, Jonathan and Geoffrey Morgan of Ann Arbor Michigan. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 22, 2009 Category: Cytology Authors: Robert B. Jennings Tags: In Memoriam Source Type: journals

MLP: A stress sensor goes nuclearEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The sarcomeric Z-disc is one of the most complex macromolecular structures in biology . Some of its constituents have important structural functions and an increasing number of recent publications point to additional, previously unexpected features. A new view is now emerging, whereby Z-disc proteins are involved as important intra- and intercellular signaling nodes . Translocation of Z-disc proteins to the nucleus and probably to the M-band as well as to other compartments, their interaction with additional signaling molecules and ability to facilitate macromolecular protein complexes are only a few properties to indicate...
Source: Journal of Molecular and Cellular Cardiology - July 20, 2009 Category: Cytology Authors: Sylvia Gunkel, Jörg Heineke, Denise Hilfiker-Kleiner, Ralph Knöll Tags: Editorial Source Type: journals

The beneficial effects of postinfarct cytokine combination therapy are sustained during long-term follow-upEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: We have previously reported that administration of granulocyte colony-stimulating factor (G-CSF)+Flt-3 ligand (FL) or G-CSF+stem cell factor (SCF) improves left ventricular (LV) function and halts LV remodeling at 35 d after myocardial infarction (MI). In the current study, we investigated whether these beneficial effects are sustained in the long term — an issue of fundamental importance for clinical translation. Mice undergoing a 30-min coronary occlusion followed by reperfusion received vehicle (group I), G-CSF+FL (group II), G-CSF+SCF (group III), or G-CSF alone (group IV) starting 4 h after reperfusion a...
Source: Journal of Molecular and Cellular Cardiology - July 16, 2009 Category: Cytology Authors: Santosh K. Sanganalmath, Adam B. Stein, Yiru Guo, Sumit Tiwari, Greg Hunt, Robert J. Vincent, Yiming Huang, Arash Rezazadeh, Suzanne T. Ildstad, Buddhadeb Dawn, Roberto Bolli Tags: Regular Articles Source Type: journals

Insulin inhibits leukocyte–endothelium adherence via an Akt-NO-dependent mechanism in myocardial ischemia/reperfusionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to test the hypothesis that insulin inhibits adherence of polymorphonuclear leukocytes (PMNs) to endothelial cells in myocardial ischemia/reperfusion (MI/R) and to investigate the underlying mechanisms. Anesthetized rabbits were subjected to MI/R (45 min/4 h) and randomly received saline, glucose-insulin-potassium (GIK) or GK respectively (2 mL/kg/h, i.v.). In vitro study was performed on cultured endothelial cells subjected to simulated ischemia/reperfusion. In vivo treatment with GIK but not GK attenuated myocardial injury as evidenced by reduced plasma creatine kinase activity, myocardial apop...
Source: Journal of Molecular and Cellular Cardiology - July 16, 2009 Category: Cytology Authors: Jia Li, Feng Wu, Haifeng Zhang, Feng Fu, Lele Ji, Ling Dong, Qiuxia Li, Wenchong Liu, Yuan Zhang, Anlin Lv, Haichang Wang, Jun Ren, Feng Gao Tags: Regular Articles Source Type: journals

Molecular and biophysical mechanisms of Ca2+ sparklets in smooth muscleEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: In this article, we review the biophysical basis and functional implications of a novel Ca2+ signal (called “Ca2+ sparklets”) produced by Ca2+ influx via L-type Ca2+ channels (LTCCs) in smooth muscle. Ca2+ sparklet activity is bimodal. In low activity mode, Ca2+ sparklets are produced by random, brief openings of solitary LTCCs. In contrast, small clusters of LTCCs can function in a high activity mode that creates sites of continual Ca2+ influx called “persistent Ca2+ sparklets”. Low activity and persistent Ca2+ sparklets contribute to Ca2+ influx in arterial, colonic, and venous smooth muscle. Targeting ...
Source: Journal of Molecular and Cellular Cardiology - July 16, 2009 Category: Cytology Authors: Luis F. Santana, Manuel F. Navedo Tags: Reviews Source Type: journals

Local control of Ca2+-induced Ca2+ release in mouse sinoatrial node cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Emerging evidence from large animal models implicates Ca2+ regulation, particularly intracellular sarcoplasmic reticulum (SR) Ca2+ release, as essential for sinoatrial node (SAN) automaticity. However, despite the apparent importance of SR Ca2+ release to SAN cell function it is uncertain how SR Ca2+ release is controlled in SAN cells from mouse. Understanding mouse SAN SR Ca2+ release mechanism will allow improved understanding of results in studies on SAN from genetic mouse models of Ca2+ homeostatic proteins. Here we investigated the functional relationship between sarcolemmal Ca2+ influx and SR Ca2+ release a...
Source: Journal of Molecular and Cellular Cardiology - July 15, 2009 Category: Cytology Authors: Biyi Chen, Yuejin Wu, Peter J. Mohler, Mark E. Anderson, Long-Sheng Song Tags: Regular Articles Source Type: journals

Transcriptional remodeling of rapidly stimulated HL-1 atrial myocytes exhibits concordance with human atrial fibrillationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: During atrial fibrillation (AF), rapid stimulation causes atrial remodeling that increases arrhythmia susceptibility. Using an established atrial (HL-1) myocyte model, we investigated the transcriptional profile associated with early atrial myocyte remodeling. Spontaneously contracting HL-1 cells were cultured in the absence and presence of rapid stimulation for 24 h and RNA harvested for microarray analysis. We identified 758 genes that were significantly altered with rapid stimulation (626 up- and 132 down-regulated). Results were confirmed using real-time quantitative RT-PCR for selected genes based on phys...
Source: Journal of Molecular and Cellular Cardiology - July 15, 2009 Category: Cytology Authors: Lisa C. Mace, Liudmila V. Yermalitskaya, Yajun Yi, Zhenjiang Yang, Ashley M. Morgan, Katherine T. Murray Tags: Regular Articles Source Type: journals

High-fat feeding in cardiomyocyte-restricted PPARδ knockout mice leads to cardiac overexpression of lipid metabolic genes but fails to rescue cardiac phenotypesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Peroxisome proliferator-activated receptor δ (PPARδ) is an essential determinant of basal myocardial fatty acid oxidation (FAO) and bioenergetics. We wished to determine whether increased lipid loading affects the PPARδ deficient heart in transcriptional regulation of FAO and in the development of cardiac pathology. Cardiomyocyte-restricted PPARδ knockout (CR-PPARδ−/−) and control (α-MyHC-Cre) mice were subjected to 48 h of fasting and to a long-term maintenance on a (28 weeks) high-fat diet (HFD). The expression of key FAO proteins in heart was examined. Serum lipid profiles, cardiac pathology, and c...
Source: Journal of Molecular and Cellular Cardiology - July 12, 2009 Category: Cytology Authors: Yuquan Li, Lihong Cheng, Qianhong Qin, Jian Liu, Woo-kuen Lo, Lowrence A. Brako, Qinglin Yang Tags: Regular Articles Source Type: journals

Constitutive CaMKII activity regulates Na+ channel in rat ventricular myocytesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, CaMKII constitutively regulates cardiac Na+ channel and this regulatory mechanism is important for the maintenance of Na+ channel characteristics under physiological conditions. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 8, 2009 Category: Cytology Authors: Jin-Young Yoon, Won-Kyung Ho, Seong-Tae Kim, Hana Cho Tags: Regular Articles Source Type: journals

Kappa and delta opioid receptor signaling is augmented in the failing heartEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study found an augmented negative inotropic and lusitropic response to administration of agonists selective for the kappa opioid receptor and delta opioid receptor in the failing heart that was mediated by a pertussis toxin-sensitive G-protein. The augmented decrease in cardiac function was manifested by increased inhibition of cAMP accumulation and the amplitude of the systolic Ca2+ transient. Furthermore, increased depression of cardiac function and of two important second messengers, cAMP and intracellular Ca2+, were independent of changes in cardiac opioid peptide or receptor expression. Thus, the cardiomyopathy-i...
Source: Journal of Molecular and Cellular Cardiology - June 30, 2009 Category: Cytology Authors: Craig Bolte, Gilbert Newman, Jo El J. Schultz Tags: Regular Articles Source Type: journals

Regulation of basal and reserve cardiac pacemaker function by interactions of cAMP-mediated PKA-dependent Ca2+ cycling with surface membrane channelsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Decades of intensive research of primary cardiac pacemaker, the sinoatrial node, have established potential roles of specific membrane channels in the generation of the diastolic depolarization, the major mechanism allowing sinoatrial node cells to generate spontaneous beating. During the last three decades, multiple studies made either in the isolated sinoatrial node or sinoatrial node cells have demonstrated a pivotal role of Ca2+ and, specifically Ca2+ release from sarcoplasmic reticulum, for spontaneous beating of cardiac pacemaker. Recently, spontaneous, rhythmic local subsarcolemmal Ca2+ releases from ryano...
Source: Journal of Molecular and Cellular Cardiology - June 30, 2009 Category: Cytology Authors: Tatiana M. Vinogradova, Edward G. Lakatta Tags: Reviews Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 29, 2009 Category: Cytology Source Type: journals