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146 records returned

Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environmentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Simple and rapid methods for the quantification of DNA, RNA and proteins using specific fluorescent dyes are proposed for the comparison and monitoring of microbial communities from the environment. The purpose of this study was the use of straightforward in situ methods which voided the need for preservation of samples and the risk of potential degradation and quantitative changes during transportation. Aside from this, methods used to obtain information on environmental microbial communities are generally time-consuming and present certain difficulty above all when working on solid substrates such as soils and rocks. New...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: M. CARMEN PORTILLO, JUAN M. GONZALEZ Tags: Original Articles Source Type: journals

Suitability of intergenic spacer or internal transcribed spacer microsatellite-primed pcr for the identification of rhizoctonia solani and some phytofungiEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The intergenic spacer (IGS) region or internal transcribed spacer (ITS) region were used in pair-combinations with microsatellite-primed polymerase chain reaction (MP-PCR) primers to establish whether additional polymorphisms can be yielded. A total of 24 Rhizoctonia solani isolates representing 13 anstomosis groups and 9 different fungal species isolate were recovered from different areas and hosts. Forty different primer combinations were tested for their ability to provide discrete bands and individual isolates' readily interpretable and reproducible IGS/ITS-MP-PCR profiles. Both approaches produced highly reproducible ...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: K.A. ABD-ELSALAM, J.-R. GUO, M.A. MOSLEM, A.H. BAHKALI, J.-A. VERREET Tags: Original Articles Source Type: journals

Persistence of salmonella spp. on chicken skin after exposure to an italian marinade*Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A series of experiments with chicken skin was undertaken to determine the effect of an Italian marinade on persistence of Salmonella spp. during refrigerated storage and marinating. Chicken skin was inoculated with 0.4 to 3.7 log of multiple antibiotic resistant strains of Salmonella Typhimurium (n = 3), Kentucky (n = 1) or Hadar (n = 1). Chicken skin was then exposed to the Italian marinade for 4 or 24 h at 6C to simulate normal marinating conditions of consumers. The persistence of Salmonella spp. on chicken skin was reduced (P < 0.05) by the Italian marinade with a greater reduction observed at 24 h than at 4 h of marin...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: THOMAS P. OSCAR, MANPREET SINGH Tags: Original Articles Source Type: journals

Comparative analysis of methods for the purification of dna from low-biomass samples based on total yield and conserved microbial diversityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This report will hopefully inspire investigators from various industries (pharmaceutical, ecological, medical, semiconductor, etc.) who find themselves in the initial phases of large-scale studies to devote a significant effort into optimizing sample extraction protocols to achieve the most accurate information. (Source: Journal of Rapid Methods and Automation in Microbiology)
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: MYRON T. LA DUC, SHARIFF OSMAN, KASTHURI VENKATESWARAN Tags: Original Articles Source Type: journals

Evaluation of cord formation in kinyoun-stained smears of mgit cultures as a rapid identification method for mycobacterium tuberculosis complexEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Tuberculosis (TB) is a major cause of morbidity and mortality worldwide. One hundred and nineteen acid-fast bacilli-positive smears for Mycobacterium Growth Indicator Tube cultures from 119 patients were examined by microscopy for the presence of cord formation. The results were compared with those of the traditional TB identification method, IS6110 polymerase chain reaction (PCR), and the Capilia TB assay which uses a monoclonal antibody to identify. With the traditional TB identification method, 57 of these 119 specimens were determined to be positive for Mycobacterium tuberculosis complex, and the organisms in the remai...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: JUN-REN SUN, SHAN-SHAN HSIEH, SHIH-YI LEE, JANG-JIH LU Tags: Original Articles Source Type: journals

Determination of indicator bacteria in pharmaceutical samples by multiplex pcrEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rapid and sensitive detection techniques for indicator pathogens are important in pharmaceutical industry. However, common detection methods rely on bacterial culture in combination with biochemical tests, a process that typically takes 5[ndash]6 days to complete. Thus, the aim of this study was to develop a multiplex polymerase chain reaction (mPCR) assay for simultaneous detection and identification of four indicator pathogenic bacteria in a single reaction. Specific primers for indicator bacteria, namely Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosaand Salmonella, were applied to allow simultaneous det...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: S. FARAJNIA, M. HASSAN, S. HALLAJ NEZHADI, L. MOHAMMADNEJAD, M. MILANI, F. LOTFIPOUR Tags: Original Articles Source Type: journals

Multiplex detection of escherichia coli and salmonella enteritidis by using quantum dot-labeled antibodiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we demonstrated the simultaneous detection of Escherichia coli and Salmonella enteritidis, by coupling immunomagnetic separation (IMS) with quantum dots (QDs) labeling. QDs having different emission wavelengths were conjugated with anti-E. coli and anti-Salmonella antibodies. QD[ndash]antibody conjugates were used to label immunomagnetically separated bacteria and the fluorescence intensities were measured for enumerations of both species. The concentrations of primary antibodies used in IMS, the ratio of QDs to antibodies during the conjugation and the concentration of QD[ndash]antibody conjugates used in l...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: FAHRIYE CEYDA DUDAK, İSMAİL HAKKI BOYACI Tags: Original Articles Source Type: journals

A new fluorogenic assay for monitoring and determining planktonic and biofilm forms of pseudomonas aeruginosa viable count in vitroEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A new method was developed to rapidly monitor the Pseudomonas aeruginosa viable counts using alamar blue (AB). The 96-well microtiter plates were used to perform the assay. This procedure is based on fluorogenic measurement as a result of reduction of nonfluorescent AB to red fluorescent form by the viable cells of P. aeruginosa. The correlation between conventional plate count and fluorogenic AB method was highly satisfactory for quantification of planktonic (R2 = 0.9487) and biofilm cells of P. aeruginosa (R2 = 0.9296). The new fluorogenic method can rapidly monitor Pseudomonas aeruginosa counts in vitro with a high corr...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: WALID F. ELKHATIB, AYMAN M. NOREDDIN Tags: Original Articles Source Type: journals

Direct fluorescent antibody-direct viable count and polymerase chain reaction detection limit for the identification of vibrio cholerae o1 in mussels (mytilus edulis)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Vibrio cholerae O1 is natural to the aquatic environment and can cause gastrointestinal infections when it is consumed from contaminated bivalves. Under unfavorable conditions, this bacterium enters into a viable but nonculturable state. Immunofluorescence and polymerase chain reaction (PCR) methods were a useful alternative for detecting this microorganism without a pre-enrichment step. We investigated the detection limit of the direct fluorescent antibody (DFA)-direct viable count (DVC) and PCR techniques for the identification of V. cholerae O1 in mussel (Mytilus edulis) samples. When 103 cfu/mL V. cholerae O1 were inoc...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: S.R. PERESSUTTI, V. JURQUIZA, S. GONZÁLEZ-FRAGA, M. PICHEL, N. BINSZTEIN, M. COSTAGLIOLA Tags: Original Articles Source Type: journals

Application of a colony pcr technique with fung's double tube method for rapid detection and confirmation of clostridium perfringensEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A colony polymerase chain reaction (PCR) technique was applied with an established Fung's double tube (FDT) method for rapid detection and confirmation of Clostridium perfringens. Published sequences of PCR primers for C. perfringens alpha toxin gene were used and PCR conditions were optimized. From the detection of C. perfringens by FDT tube to the confirmation by a colony PCR assay took as short as 16[ndash]18 h. The method was applied to 147 isolates of anaerobic sulfite reducing bacteria isolated from foods, sewages and animal clinical specimens. The results were compared with standard methods for the confirmation of C...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: CHAOWAREE RUENGWILYSUP, CHANITCHOTE DETVISITSAKUN, NAIYANA AUMYAT, D.Y.C. FUNG Tags: Original Articles Source Type: journals

A novel method for assessment of 16s rrna gene copy number in bacterial genomes by pulsed-field gel electrophoresis and pcr amplificationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
16S rRNA gene (rrn) copy number in bacterial genomes is indicative of ecological strategies of bacteria and is critical for quantification of bacterial abundance in mixed populations using rrn-based approaches. For accurate assessment of rrn copies, a novel technical strategy by means of pulsed-field gel electrophoresis and polymerase chain reaction amplification analysis was introduced. Experimental and in silico analysis on a test bacterial culture Caulobacter crescentus proved it to be simple, effective, accurate and a good alternative to traditional time-consuming methods. This method can be used for routine determinat...
Source: Journal of Rapid Methods and Automation in Microbiology - September 1, 2009 Category: Microbiology Authors: YONGHUI ZENG, NIANZHI JIAO Tags: Original Articles Source Type: journals

EditorialEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Rapid Methods and Automation in Microbiology)
Source: Journal of Rapid Methods and Automation in Microbiology - August 31, 2009 Category: Microbiology Tags: Editorial Source Type: journals

Nested automated ribosomal intergenic spacer analysis: a rapid and accurate method for comparison of bacterial community compositionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nested automated ribosomal intergenic spacer analysis (ARISA) was used to examine the community structure of epilithic biofilms in freshwater streams experiencing different levels of human impact. This molecular fingerprinting technique generated reproducible profiles of bacterial community structure that varied significantly between stream sites. Nested ARISA was determined to be a cost-effective, high-throughput approach to assess bacterial community composition from very small sample volumes, requiring little sampling effort and without the need for taxonomic identification of individual organisms. In combination with m...
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: G. LEAR, G.D. LEWIS Tags: Original Articles Source Type: journals

Large-volume filtration for recovery and concentration of escherichia coli o157:h7 from ground beef*Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This report describes a novel three-stage filtration system based on a leukocyte removal filter, a glass fiber prefilter, and a membrane capture filter. Data are presented on factors (e.g., particle size, bacteria binding, pH) affecting filtration performance and protocol design. Escherichia coli O157:H7 at less than 1 cfu/g were quantitatively recovered from 10 g of stomached ground beef in 15 min, and detected on selective media within 24 h. The methodology presented in this study allows the rapid concentration of Escherichia coli O157:H7 from the large volumes of stomached ground beef. The primary application is in rapi...
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: J.D. BREWSTER Tags: Original Articles Source Type: journals

A high throughput screening method for 1,3-dihydroxyacetone-producing bacterium by cultivation in a 96-well microtiter plateEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
1,3-Dihydroxyacetone (DHA) is used extensively in the cosmetic industry, and is the main active ingredient in all sunless tanning skincare preparation. In order to more efficiently and rapidly screen suitable strains or mutants for production of DHA, a high throughput screening method for DHA-producing bacterium by cultivation in a 96-well microtiter plate was developed. With this screening method, more than 100 strains that were able to convert glycerol to DHA were isolated from soil samples, and a mutant of Gluconobacter oxydans ZJB-605 that displayed the highest DHA productivity was obtained. The practical application o...
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: Z.-C. HU, Y.-G. ZHENG Tags: Original Articles Source Type: journals

Serological attraction of nontoxic egg component to staphylococcal anti-enterotoxinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Raw whole liquid and dried eggs which were purported to contain staphylococcal enterotoxin were analyzed by a number of enzyme-linked immunosorbent assay (ELISA)-based methods. The initial evaluation was to establish whether the purported positive ELISA reactions were a result of toxin-anti-enterotoxin serological activity. A secondary consideration was to determine whether the putative protein occurred in the yolk and/or white portions of the eggs. A manual polyvalent detection system, manual monovalent ELISA and an automated polyvalent enzyme-linked fluorescent immunoassay were used to investigate this component in eggs....
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: REGINALD W. BENNETT Tags: Original Articles Source Type: journals

Rapid detection of listeria monocytogenes in ground turkey by immunomagnetic separation and polymerase chain reactionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study rapid, specific and sensitive IMS method was used to determine the prevalence of L. monocytogenes in fresh ground turkey and PCR technique was used for the verification of the L. monocytogenes isolates. (Source: Journal of Rapid Methods and Automation in Microbiology)
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: N.D. AYAZ, I. EROL Tags: Original Articles Source Type: journals

Simultaneous direct detection of salmonella spp., listeria monocytogenes and escherichia coli o157 in milk samples by magnetic extraction and multiplex pcrEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of our work was to develop a new molecular method for the simultaneous detection of Salmonella spp., Listeria monocytogenes and Escherichia coli O157 directly in milk samples. Three specific target sequences were chosen for a multiplex polymerase chain reaction (PCR) assay: a 155-bp region of the Salmonella spp. tetrathionate reductase (ttr) locus; a 173-bp region of the L. monocytogenes listeriolysin O gene (hlyA); a 217-bp region of the E. coli O157 lipopolysaccharide gene (rfbE). An internal amplification control was also included to detect PCR inhibition. In addition, a magnetic-based extraction method was also...
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: ENRICA OMICCIOLI, GIULIA AMAGLIANI, GIORGIO BRANDI, IAN J. BRUCE, MAURO MAGNANI Tags: Original Articles Source Type: journals

Comparison of three different methods for the isolation of bacteriocin-like inhibitory substances from bifidobacterium infantis bcrc 14602Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this research was to compare three different methods for the isolation of bacteriocin-like inhibitory substances (BLIS) from Bifidobacterium infantis BCRC 14602 (Bioresource Collection and Research Center) for their ease of use and reliability. Ammonium sulfate with 80% saturation of the neutralized cell-free supernatant resulted in 80% of total activity and a 4.56-fold increase in specific activity. After dialysis, the specific activity increased 76-fold, but total activity recovered decreased to 8%. In the second method, adsorption and desorption of the BLIS onto/from the producer cells, the adsorption o...
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: AHMAD CHEIKHYOUSSEF, NATASCHA POGORI, HAIQIN CHEN, JIANXIN ZHAO, JIAN TANG, WEI CHEN, HAO ZHANG Tags: Original Articles Source Type: journals

Identification of proteins involved in infectivity and enterotoxin production in enterobacter sakazakiiEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Enterobacter sakazakii is a gram-negative rod bacterium that was formerly known as "yellow-pigmented Enterobacter cloacae" until 1980. It is an emerging opportunistic pathogen associated with bacterial meningitis in immunocompromised neonates who ingested contaminated powdered infant formula. In several E. sakazakii-related outbreaks and sporadic cases, powdered infant formula was epidemiologically or microbiologically established as the source of infection. The International Commission on Microbiological Specifications for Foods has ranked E. sakazakii as a "severe hazard for restricted populations." However, the organism...
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: JIELIN YANG, LIMING WEI, MING GU, XIAOMING FANG, PENYUAN YANG Tags: Original Articles Source Type: journals

Rapid detection of brucella abortus by a novel proximity ligation-based loop-mediated isothermal amplification methodEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we present the novel proximity ligation-based loop-mediated isothermal amplification (P-LAMP) method for Brucella detection; this is the first time to combine the monoclonal antibody for identify microbe and LAMP method for high performance amplification DNA. The genomic DNA extraction procedure is not needed and a water-bath boiler is the only equipment required to complete the detection process. The P-LAMP method is useful for food safety pathogen detection in developing countries. (Source: Journal of Rapid Methods and Automation in Microbiology)
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: CHENGGANG ZHU, XIAOYAN DENG, FENG SHI Tags: Original Articles Source Type: journals

Identifying aceticlastic and hydrogenotrophic methanogens in psychrophilic and mesophilic granular sludges treating synthetic sewage by means of fish and cslmEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, temperature influence on aceticlastic and hydrogenotrophic methanogen groups was evaluated using FISH coupled with CSLM. (Source: Journal of Rapid Methods and Automation in Microbiology)
Source: Journal of Rapid Methods and Automation in Microbiology - June 5, 2009 Category: Microbiology Authors: CIGDEM YANGIN GOMEC, VEYSEL EROGLU, PETER A. WILDERER Tags: Original Articles Source Type: journals

Development of multiplex pcr assays for detection of antimicrobial resistance genes in escherichia coli and enterococciEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we focused on the simultaneous detection of five and three most commonly found resistance genes in Escherichia coli and enterococci isolates, respectively. Our research has shown that the mPCR method could be expanded to include the determination of other antibiotic resistance genes of interest. The system described here can decrease PCR reagent cost by multiple resistance genes detection in each reaction tube. The high throughput and cost-effective mPCR system developed in this study could provide a powerful tool for more accurate detection of various antimicrobial resistance genes associated with E. coli a...
Source: Journal of Rapid Methods and Automation in Microbiology - June 1, 2009 Category: Microbiology Authors: WIPAWADEE SIANGLUM, KANOKWAN KITTINIYOM, POTJANEE SRIMANOTE, WIJIT WONGLUMSOM Tags: Original Articles Source Type: journals

Identification of cellulolytic bacteria isolated from the termite coptotermes curvignathus (holmgren)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of the present research was to isolate and identify cellulolytic bacteria from the gut of the local termite Coptotermes curvignathus (Holmgren) present in the vicinity of the University of Putra Malaysia. The isolates were cultured in a medium containing carboxymethyl-cellulose and cellobiose. The bacterial species were tentatively identified by using the Biolog reader as well as the Bergey's manual and later confirmed by 16S rRNA sequence homology. The species were all novel strains and identified as Bacillus cereus strain Razmin A, Enterobacter aerogenes strain Razmin B, Enterobacter cloacae strain Razmin C, Chry...
Source: Journal of Rapid Methods and Automation in Microbiology - March 2, 2009 Category: Microbiology Authors: M. RAMIN, A.R. ALIMON, N. ABDULLAH Tags: Original Articles Source Type: journals

Rapid detection of germinating bacillus cereus cells using fluorescent in situ hybridizationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study presents an experimental design in order to find the best combination of germination conditions leading to a rapid and detectable fluorescent in situ hybridization (FISH) signal from Bacillus cereus spores present in pure cultures and milk samples. B. cereus ATCC 14579 and HER 1414 were incubated in 20 different growth media by using a combination of various germinants such as sugars, amino acids and dipicolinic acid. Also, three different germination factors were tested: incubation temperature, inoculum concentration and a heat shock treatment. Permeabilization procedure and hybridization time were optimized on...
Source: Journal of Rapid Methods and Automation in Microbiology - March 2, 2009 Category: Microbiology Authors: CHRISTIAN LAFLAMME, LOUIS GENDRON, NATHALIE TURGEON, GENEVIEVE FILION, JIM HO, CAROLINE DUCHAINE Tags: Original Articles Source Type: journals

Identification of new target sequences for pcr detection of vibrio parahaemolyticus by genome comparisonEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A genome comparison method was used to identify specific target sequences for the polymerase chain reaction (PCR) detection of Vibrio parahaemolyticus, and the CDS value of this bacterium was compared with that of 139 other bacterial genomes. It was found that 20 CDS of V. parahaemolyticus were relatively specific according to their E value in BLAST (a new tool for comparing protein and nucleotide sequences), and four of them were selected for the design of PCR primers. There were positive amplification products of these four pairs of primers from nine V. parahaemolyticus strains, whereas there were no amplification produc...
Source: Journal of Rapid Methods and Automation in Microbiology - March 2, 2009 Category: Microbiology Authors: DONG-SHENG ZHU, MIN ZHOU, YI-LING FAN, XIAN-MING SHI Tags: Original Articles Source Type: journals

Development and evaluation of a loop-mediated isothermal amplification method for detecting escherichia coli o157 in raw milkEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study, we report the performance of a loop-mediated isothermal amplification (LAMP) assay detecting foodborne pathogen Escherichia coli O157. Three pairs of primers were specially designed for recognizing eight distinct sequences of rfbE gene. Time and temperature conditions for amplification of E. coli O157 were optimized to be 40 min at 65C. The LAMP assay gave artificially contaminated raw milk sample detection limit level of 410 cfu/mL which corresponds to three to five cells per reaction tube, while the detection level of conventional polymerase chain reaction was 4.1 × 104 cfu/mL. Data on naturally co...
Source: Journal of Rapid Methods and Automation in Microbiology - March 2, 2009 Category: Microbiology Authors: DEGUO WANG, FEI LIU, GUICHENG HUO, DAXI REN, YONGGANG LI Tags: Original Articles Source Type: journals

Enumerating chromogenic agar plates using the color qcount automated colony counterEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study compared colony counts of mixed bacterial cultures on chromogenic spread plates using manual and Color QCount (Spiral Biotech, Inc., Norwood, MA) automated methods. Inoculum levels spanned 30[ndash]300 cfu/mL, 26 agar types were used and 581 plates were analyzed. Plates were prepared according to manufacturers' instructions, manually counted once by two scientists and counted in duplicate automatically. The correlation coefficient comparing automated and manual counts for the pooled population of data was 0.987. The slope and intercept for the linear regression line were 1.0067 and 0.031, respectively. The mean ...
Source: Journal of Rapid Methods and Automation in Microbiology - March 2, 2009 Category: Microbiology Authors: EILEEN GARRY, GRACE OUATTARA, PATRICK WILLIAMS, MEREDITH PESTA Tags: Original Articles Source Type: journals

Development of a multiwell antagonistic activity assay for the detection of bacteriocin production by lactic acid bacteriaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A total of 300 strains of lactic acid bacteria (LAB) were screened for the production of bacteriocins active against Listeria innocua or Escherichia coli by using two different techniques: the conventional well-diffusion assay and a newly developed one based on turbidity measurement of the growth of an indicator bacterium in the neutralized cell-free supernatant of the putative bacteriocin-producing strain. The latter technique, designated as the multiwell antagonistic activity assay (MW3A), offers advantages over the previously known methods. Notably, it allows testing simultaneously a large number of LAB for the producti...
Source: Journal of Rapid Methods and Automation in Microbiology - March 2, 2009 Category: Microbiology Authors: KAOUTAR YAAKOUBI, NOREDDINE BENKERROUM, FLORENT WIOROWSKI, FRANÇOISE SANSON, JULIEN HAYDERSAH, ISABELLE CHEVALLIER Tags: Original Articles Source Type: journals

Rapid detection of mycobacterium tuberculosis in lung tissue using a fiber optic biosensorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
There is no rapid diagnostic technique at medical examiners' offices to determine if a decedent is infected with Mycobacterium tuberculosis. Present diagnostic testing requires at least 1 month for results. The RAPTOR, a portable, automated fiber optic evanescent wave biosensor, was used as the platform to develop more rapid assays to detect M. tuberculosis from lung tissue. Positive biosensor detection was obtained 80% of the time at cell concentrations of 106 cells/mL, 96% of the time at 107 cells/mL, and 99% of the time at 108 cells/mL of live attenuated M. tuberculosis (ATCC 25177) suspended in phosphate-buffered salin...
Source: Journal of Rapid Methods and Automation in Microbiology - March 2, 2009 Category: Microbiology Authors: KIMBERLY A. DENTON, MARIANNE F. KRAMER, DANIEL V. LIM Tags: Original Articles Source Type: journals

CHEF PROCEDURES: A RAPID HIGH-TEMPERATURE METHOD FOR SAMPLE PREPARATION, A HIGH VOLTAGE HEPES BUFFER SYSTEM AND THE USE OF NUSIEVE® AGAROSEEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A rapid, high-temperature method of sample preparation from gram-negative bacteria for contour-clamped homogenous electric field (CHEF) electrophoresis is described, which utilizes a diethylpyrocarbonate nuclease inactivation step for preventing latent degradation of DNA. Also described is a simple 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer system that permits high-voltage CHEF electrophoresis in standard apparatus while preventing the strand scission events associated with tris(hydroxymethyl)aminomethane buffers. Finally, the use of NuSieve agarose in combination with standard analytical agarose was compare...
Source: Journal of Rapid Methods and Automation in Microbiology - March 2, 2009 Category: Microbiology Authors: THOMAS G. KINSCHERF, MEE-NGAN YAP, AMY O. CHARKOWSKI, DAVID K. WILLIS Tags: Original Articles Source Type: journals

Simplified capacitance monitoring for the determination of campylobacter spp. growth ratesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Capacitance monitoring is commonly used as an efficient means to generate growth curves of bacterial pathogens. However, the use of capacitance monitoring with Campylobacter spp. was previously determined to be difficult due to the complexity of the required media. We investigated capacitance monitoring using a simplified medium for the efficient and reproducible construction of growth curves for Campylobacter spp. It was determined that Campylobacter spp. should initially be propagated on Mueller Hinton plates in a microaerobic atmosphere (5% O2, 10% CO2, 85% N2) at 37C for 24 h, followed by transfer to Mueller Hinton bip...
Source: Journal of Rapid Methods and Automation in Microbiology - March 2, 2009 Category: Microbiology Authors: E. DEANN AKINS, PATRICIA L. RULE, MICHELLE R. KEENER, HOLLY S. SELLERS, J. ERIC LINE, MUSTAFA SIMMONS, MARK A. HARRISON, KELLI L. HIETT Tags: Original Articles Source Type: journals

EditorialEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Rapid Methods and Automation in Microbiology)
Source: Journal of Rapid Methods and Automation in Microbiology - March 1, 2009 Category: Microbiology Authors: DANIEL Y.C. FUNG Tags: Editorial Source Type: journals

Molecular characterization of listeria monocytogenes strains harboring listeria innocua putative transcriptional regulator gene lin0464Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, through examination of a large collection (n = 84) of Listeria monocytogenes strains, we identified four serovar 4c strains (i.e., RM3894, RM3899, RM3901 and RM3905) that cross-reacted with Listeria innocua putative transcriptional regulator gene lin0464 primers. We then investigated these four unusual strains by polymerase chain reaction (PCR) with primers for several L. monocytogenes species-, virulence- and group-specific genes and a newly identified L. innocua-specific transcriptional regulator lin2455 gene. The fact that the four strains were recognized by L. monocytogenes inlA, lmo0733, inlJ, lmo2672 a...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: DONGYOU LIU, MARK L. LAWRENCE, ANTHONY D. HITCHINS Tags: Original Articles Source Type: journals

DEVELOPMENT OF A MULTIPLEX PCR METHOD FOR DETECTION OF THE GENES ENCODING 16S rRNA, COAGULASE, METHICILLIN RESISTANCE AND ENTEROTOXINS IN STAPHYLOCOCCUS AUREUSEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A multiplex polymerase chain reaction (PCR) method was developed for simultaneous detection of the genes encoding methicillin resistance (mecA), SEs A, B and C (sea, seb and sec), coagulase (coa), and 16S rRNA. The primers for amplification of the 16S rRNA gene were specific for Staphylococcus spp., and the primers for coa were specific for Staphylococcus aureus. Based on the results, the multiplex PCR was accomplished at an optimal Mg2+concentration of 1.0 mM and at an annealing temperature of 56C. This multiplex PCR method was performed with 71 strains of S. aureus and 51 strains of six other bacterial species. Among the...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: YILING FAN, FENG PAN, GEORGE C. PAOLI, YONGHONG XIAO, HAIHUI SHENG, XIANMING SHI Tags: Original Articles Source Type: journals

Rapid multiplex pcr assay for simultaneous detection of major antibiotic resistance determinants in clinical isolates of bacteroides fragilisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study developed a rapid and cost-effective multiplex polymerase chain reaction assay (M-PCR) for simultaneous detection of major clinically relevant antibiotic resistance genes in Bacteroides fragilis. The M-PCR optimized amplification conditions with primers designed for detection of five resistance genes: carbapenems (cfiA) and cephalosporins (cepA), clindamycin (ermF), metronidazole (nimA-F) and tetracycline (tetQ), plus a set of primers for the B. fragilis 16S rRNA gene (positive control). An initial single PCR was performed for each gene, followed by a gradient PCR to determine the optimal PCR conditions for each...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: LILIAN PUMBWE, MATTHEW CURZON, HANNAH M. WEXLER Tags: Original Articles Source Type: journals

Detection of campylobacter from broiler carcass rinse samples utilizing the tecra visual immunoassay (via)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Poultry meat is considered to be a major vector of transmission of Campylobacter, either directly, through consumption of poorly prepared product or cross-contamination, or indirectly, through introduction of the bacterium into the food production environment. Efficient detection of Campylobacter is intrinsic to the management of the pathogen during poultry production. The TECRA Campylobacter Visual Immunoassay (CAMVIA) protocol, enrichment in a proprietary TECRA Campylobacter enrichment broth followed by an ELISA, was compared with a conventional cultural method, with enrichment in Bolton medium (containing lysed horse bl...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: J.S. BAILEY, P. FEDORKA-CRAY, L.J. RICHARDSON, N.A. COX, J.M. COX Tags: Original Articles Source Type: journals

Rapid identification of pseudomonas spp. from aquatic samples using terminal restriction fragment length polymorphism analysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The genus Pseudomonas includes several species with an important biotechnological potential. Here we present a molecular approach for the analysis of the diversity of Pseudomonas spp. in aquatic samples based on the amplification of 16S rDNAs using taxon-specific primers, followed by multienzymatic restrictions and subsequent screening of terminal restriction fragments using capillary electrophoresis. This method, tested on seawater, groundwater and aquatic sediments, allows the identification of Pseudomonas spp. in samples, in which culture-based approaches failed in identifying their presence. The diversity of Pseudomona...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: GIAN MARCO LUNA, ROBERTO DANOVARO Tags: Original Articles Source Type: journals

Reproducible filtration of bacteria with micromechanical filtersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
For the detection of bacteria in potable water, several kinds of biosensors could be used. However, any of them faces its physical limits when very low concentrations of bacteria should be detected. In this article, we used micromechanical filters with perforated membranes for capturing and enriching bacteria and to facilitate the detection of Escherichia coli. The results in this article show the easy and effective removal of bacteria from the surface of the microsieve membranes. By plating off the filters on LB agar plates, we could quantitatively analyze very low concentrations of bacteria and show that micromechanical ...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: ULRICH REIDT, LINA CHAUHAN, GERHARD MÜLLER, RAMONA MOLZ, PETRA LINDNER, HANS WOLF, ALOIS FRIEDBERGER Tags: Original Articles Source Type: journals

NEW PRIMERS FOR AMPLIFICATION OF THE PLANCTOMYCETES 16S rRNA GENE FROM ENVIRONMENTAL SAMPLESEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although the 16S rDNA primer set Pla-46-F/1392-R is often used to explore the planctomycetes community, the universal reverse primer 1392-R limits the capability of the forward primer Pla-46-F. Here we report two new reverse primers (Pla-1097-R and Pla-1368-R), which greatly promote the potential ability of the forward primer Pla-46-F, both theoretically and experimentally. The tests with field samples showed that PCR induced by the new primer sets both reduced mismatches to non-planctomycetes and supplemented the diversity of planctomycetes, particularly increasing the numbers of planctomycetes genera in both seawater and...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: QINGLONG SHU, NIANZHI JIAO Tags: Original Articles Source Type: journals

An antibody modified automated enzyme-linked immunosorbent assay-based method for detection of staphylococcal enterotoxin*Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Studies were conducted with an automated enzyme-linked immunosorbent assay (ELISA)-based method (Vidas, Staph enterotoxin-II [SET-II]), exhibiting an antibody capture that had undergone modification by removal of the Fc fragment on the antibody. Raw liquid or shell eggs containing a nontoxin component with an attraction to the staphylococcal antienterotoxins were studied. Prior to ELISA testing, the eggs were homogenized and extracts collected by centrifugation. Studies showed that regardless of the ELISA-based method used that utilized the unmodified antibodies with both the Fab1 + Fc fragments intact, positive (false pos...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: REGINALD W. BENNETT Tags: Original Articles Source Type: journals

Polymerase chain reaction confirmatory method for microbiological detection of brettanomyces bruxellensis in winesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Brettanomyces bruxellensis is one of the major causes of contamination in wine and is an important source of economic losses in this industry. In this work we developed a specific polymerase chain reaction (PCR) assay for the detection of B. bruxellensis to be used in the confirmation stage of the microbiological analysis. From a random amplification analysis using 40 primers in various B. bruxellensis strains and other yeasts that are generally present in must and wine, we designed the primers E09F and E09R that amplified a 450 bp product only in B. bruxellensis strains. We determined that the concentration of the PCR com...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: ANGELA CONTRERAS, FRANCISCO SALINAS, ANGÉLICA GANGA, CLAUDIO MARTÍNEZ Tags: Original Articles Source Type: journals

Application of real-time polymerase chain reaction for rapid determination of salmonella in restaurant foodsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, 96 food samples of the University restaurant of Valencia (Spain) were divided into four groups: first course; main course; accompaniment; and dessert, and were analyzed simultaneously with classical and fluorogenic polymerase chain reaction microbiological analysis. Neither of the procedures detected the presence of Salmonella in the analyzed food samples. Polymerase chain reaction applied to identify Salmonella is time-effective and reduces the amount of time required. Identification results are available 48 h earlier than classical testing. For this reason, this method is useful as a more rapid screening t...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: H. BERRADA, J.M. SORIANO, Y. PICÓ, J. MAÑES Tags: Original Articles Source Type: journals

Early diagnosis of infectious diarrhea using lactoferrin and hemoglobin markersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objectives were to differentiate groups of diarrhea and provide a rapid test that could be used in order to help health professionals to quickly manage patient care, taking into account the type of diarrhea that is attacking. Positive results for fecal Lf indicated an inflammatory condition of the intestines. Positive results for fecal Hb suggested the presence of occult blood in stools. It has a sensitivity of 0.156 ng/µL of Lf and 0.313 ng/µL of Hb. Liquid fecal samples were mixed with a mixture containing antibodies to Lf and Hb. These antibodies were conjugated to colored latex beads. Each test zone on the test d...
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: S.P. VORAVUTHIKUNCHAI, K. OKADA, T. IIDA, T. HONDA Tags: Original Articles Source Type: journals

Fung's cornerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Rapid Methods and Automation in Microbiology)
Source: Journal of Rapid Methods and Automation in Microbiology - December 1, 2008 Category: Microbiology Authors: Daniel Y. C. Fung Tags: Fung's Corner Source Type: journals

Real-time polymerase chain reaction quantification of salmonella typhimurium hila expression during agitation and static incubationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study demonstrated the utility of quantitative RT-PCR method in determining virulence gene expression under a variety of laboratory conditions. The results confirmed oxygen limitation as a potential stimulant of S. Typhimurium hilA expression, which can also be triggered by other extremes for the bacteria growth. (Source: Journal of Rapid Methods and Automation in Microbiology)
Source: Journal of Rapid Methods and Automation in Microbiology - September 3, 2008 Category: Microbiology Authors: M.M. KUNDINGER, I.B. ZABALA-DÍAZ, V.I. CHALOVA, R.W. MOORE, S.C. RICKE Tags: Original Articles Source Type: journals

RAPID DETECTION AND ENUMERATION OF CONTAMINANTS BY ATP BIOLUMINESCENCE USING THE MILLIFLEX® RAPID MICROBIOLOGY DETECTION AND ENUMERATION SYSTEMEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The Milliflex® Rapid Microbiology Detection and Enumeration System (RMDS) is an automated platform developed for the rapid detection of microbial contaminants in filterable samples. Based on membrane filtration, adenosine triphosphate bioluminescence and image analysis, RMDS gives microorganism enumeration a detection time reduced by a factor of four compared to plate count. After sample filtration and incubation step, a universal lysis solution and bioluminescence reagent are automatically sprayed across the membrane. The membrane is then analyzed in a detection tower where light emission is captured by a charged coupled...
Source: Journal of Rapid Methods and Automation in Microbiology - September 3, 2008 Category: Microbiology Authors: RENAUD CHOLLET, MONIKA KUKUCZKA, NADINE HALTER, MARILYN ROMIEUX, FREDERIC MARC, HERVE MEDER, VINCENT BEGUIN, SEBASTIEN RIBAULT Tags: Original Articles Source Type: journals

A portable raman system for the identification of foodborne pathogenic bacteriaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study presents a procedure to make use of commercial off-the-shelf components to construct a portable dispersive Raman system and evaluates it for discrimination of bacteria by surface-enhanced Raman scattering (SERS). The system consists of a semiconductor laser (784.8 nm), a fiber optic probe ([sim]135 µm focal spot), a mini spectrometer and a computer. UV-visible spectroscopy and transmission electron microscopy analysis of four silver colloid preparations produced in this study, together with the SERS spectra of Listeria innocua adsorbed on colloidal particles, indicated that silver colloids with the extinction m...
Source: Journal of Rapid Methods and Automation in Microbiology - September 3, 2008 Category: Microbiology Authors: B. STEVEN LUO, MIN LIN Tags: Original Articles Source Type: journals

Comparison of enzymatic chemiluminescent assay and standard agar plate method in the determination of bacterial viabilityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study demonstrated desirable conditions for rapid detection of viable bacteria in a liquid medium using LC assay. The possibility of using this assay for the assessment of the activity of an antimicrobial agent such as bovine lactoferrin against Pseudomonas fluorescens 33 and Escherichia coli 1649[trade], which are present in milk, was also investigated. Good correlation was observed between LC assay and standard agar plate technique. The antibacterial effect of lactoferrin was similar whether estimated using LC assay or standard agar plate technique. These results suggest that LC assay can be a rapid and useful metho...
Source: Journal of Rapid Methods and Automation in Microbiology - September 3, 2008 Category: Microbiology Authors: WOAN-SUB KIM, MD. MORSHEDUR RAHMAN, KEI-ICHI SHIMAZAKI Tags: Original Articles Source Type: journals

A rapid method for estimating viability in desiccated cells of the biocontrol agent tsukamurella paurometabola c-924Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A new method for the rapid estimation of viability in anhydrobiotic cells of the biocontrol agent Tsukamurella paurometabola C-924 was developed. The method is based on the absorbance due to hydrogen sulfide production. A linear model was obtained by plotting decimal logarithm of the corrected absorbance at 670 nm (log[Abscorrected]) versus the decimal logarithm of culturable cells (log[Xv]) (r2 = 0.9428). After comparing the results obtained by the proposed method to the ones obtained by the plate counting technique, no significant difference was observed in the number of culturable cells. The new technique, which is fast...
Source: Journal of Rapid Methods and Automation in Microbiology - September 3, 2008 Category: Microbiology Authors: ARMANDO HERNÁNDEZ, ALAIN MOREIRA, EULOGIO PIMENTEL, JORGE MARTÍNEZ, JESÚS MENA, NEYLEN DEL TORO Tags: Original Articles Source Type: journals