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International Congress in Reproductive Immunology CairnsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - November 1, 2009 Category: Allergy & Immunology Source Type: journals

PrefaceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This special issue of the journal comprises papers presented at the Sixth International Workshop on Reproductive Immunology, Immunological Tolerance and Immunology of Preeclampsia, which was held in December 2008 in Reunion Island. This meeting celebrated the tenth anniversary since the first meeting in this series, which began in 1998 in French Polynesia, with a meeting organized by Pierre-Yves Robillard (Tahiti) and Gustaaf Dekker (Australia), quickly joined by Gérard Chaouat (France). The workshop then migrated to Mauritius Island (in 2000 and 2002) and in 2004 to Reunion Island, following the professional appointments...
Source: Journal of Reproductive Immunology - November 1, 2009 Category: Allergy & Immunology Authors: Pierre-Yves Robillard, Gustaaf Dekker, Gérard Chaouat Tags: Preface Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - November 1, 2009 Category: Allergy & Immunology Source Type: journals

Early regulators in abortion and implications for a preeclampsia modelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: This paper reports a summary of our comparative analysis of the uterine expression of interleukin-23 (IL-23), IL-27 and TWEAK in the CBA/J female×DBA/2 male mouse mating combination, a model of immune-mediated early pregnancy loss. Compared with the MHC-identical CBA/J×BALB/c mating combination, which yields successful pregnancies, immunohistochemistry and qPCR in uterine tissue showed an immediate post-mating IL-27 hyper-expression after mating with DBA/2 males. Intra-uterine TWEAK expression was present in females mated with DBA/2 or Balb/c males from days 0.5 to 4.5 post-coitum (pc), peaking on day 0.5 pc to...
Source: Journal of Reproductive Immunology - October 15, 2009 Category: Allergy & Immunology Authors: Gérard Chaouat, Marie Petitbarat, Roberta Bulla, Sylvie Dubanchet, Karina Valdivia, Nathalie Ledée, Thiel Steffen, Jens Christian Jensenius, Francesco Tedesco Source Type: journals

Dysregulation of anti-angiogenic agents (sFlt-1, PLGF, and sEndoglin) in preeclampsia—a step forward but not the definitive answerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Preeclampsia (PE) is a pregnancy-specific syndrome characterized by hypertension, proteinuria and edema, which resolves on placental delivery. It is thought to be the consequence of impaired placentation due to inadequate trophoblastic invasion of the maternal spiral arteries. In PE the maternal plasma concentration of free vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) is decreased whereas the concentration of soluble fms-like tyrosine kinase-1 (sFlt-1) and of soluble endoglin (sEng) is increased. These soluble receptors may bind VEGF, PLGF and TGFβ1 and TGFβ3 in the maternal circ...
Source: Journal of Reproductive Immunology - October 15, 2009 Category: Allergy & Immunology Authors: J.M. Foidart, J.P. Schaaps, F. Chantraine, C. Munaut, S. Lorquet Source Type: journals

International Congress in Reproductive Immunology CairnsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - September 17, 2009 Category: Allergy & Immunology Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - September 17, 2009 Category: Allergy & Immunology Source Type: journals

A novel exonic variant (221delT) in the LGALS13 gene encoding placental protein 13 (PP13) is associated with preterm labour in a low risk populationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we aimed to identify markers in pro- and anti-inflammatory genes that may contribute to disease and possibly disease prediction in a low risk community setting. A prospective study was undertaken on 450 consecutive low risk primigravid patients. Blood obtained at first booking was screened for known immunological gene variants (IL4 −590, IL1B +3953, IL1RN, IL10 −1082; −819; −592 and TNFA −308; −238; +488) as well as for novel variants in the LGALS13 gene coding for placental protein 13 (PP13). The incidence of preterm labour and pre-eclampsia was 7.1% and 6.8% respectively. A novel exonic variant ...
Source: Journal of Reproductive Immunology - September 14, 2009 Category: Allergy & Immunology Authors: S. Gebhardt, N. Bruiners, R. Hillermann Source Type: journals

Decidual leucocyte populations in early to late gestation normal human pregnancyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: Most research on human decidual leucocytes to date has focused on the predominant CD56+ uterine natural killer (uNK) cell population in early pregnancy. Few reports have documented decidual leucocyte populations after 13 weeks gestation and in late pregnancy. Placental bed (decidua basalis) and non-placental bed (decidua parietalis) biopsies from normal pregnancies were taken from women undergoing termination of pregnancy in the 1st and 2nd trimesters and following Caesarean section in the 3rd trimester. Immunohistochemistry was used to quantify the numbers of decidual cells expressing CD56, CD3, CD8, CD94, NKG2A...
Source: Journal of Reproductive Immunology - August 31, 2009 Category: Allergy & Immunology Authors: P.J. Williams, R.F. Searle, S.C. Robson, B.A. Innes, J.N. Bulmer Tags: Basic Immunology Source Type: journals

CD52 expression is induced in the mouse uterus at the time of embryo implantationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract: CD52 is a GPI anchor protein present in lymphocytes, the epithelial cells of the epididymis and sperm. It has been reported that male reproductive tract CD52 induces antibodies interfering with sperm function and causes infertility. CD52 is also expressed in ovulated cumulus cells in female reproductive tissues. In the present study, we examined the distribution and the mechanism of regulation of CD52 in the uterus. CD52 expression was evaluated in uterine tissue recovered at 0.5, 4.5, 8.5 and 12.5dpc (days post-coitum). Immunohistochemistry, RT-PCR, Western blotting and gel shift analysis were performed to deter...
Source: Journal of Reproductive Immunology - August 24, 2009 Category: Allergy & Immunology Authors: K. Kumamoto, X.Z. Yang, A. Hasegawa, S. Komori, K. Koyama Tags: Basic Immunology Source Type: journals

Interleukin-11 increases invasiveness of JEG-3 choriocarcinoma cells by modulating STAT3 expressionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, activation and upregulation of STAT3 appears to be critical for the IL-11-mediated increase in invasiveness of JEG-3 cells. (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 24, 2009 Category: Allergy & Immunology Authors: Pankaj Suman, Tobias G. Poehlmann, Golla Jaya Prakash, Udo R. Markert, Satish K. Gupta Tags: Basic Immunology Source Type: journals

International Congress in Reproductive Immunology CairnsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Source Type: journals

Chairpersons, ModeratorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Author IndexEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Inhibition of ERK1/2 does not affect LIF-induced STAT3 ser727 phosphorylation in trophoblastic cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
RAS/MAPK is an intracellular signalling pathway, which is involved in the regulation of various cellular processes including proliferation and invasion in numerous cell types. ERK1/2 which is a member of this pathway, has been described controversially to interfere with signal transducer and activator of transcription 3 (STAT3) activation by phosphorylation of its serine residue. STAT3 is major inducer of trophoblast invasiveness. It has been demonstrated that Leukemia Inhibitory Factor (LIF) induces RAS and STAT3 activation. Here we have tested effects of LIF on ERK1/2 and STAT3 ser727 phosphorylation in trophoblastic cel...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: D.M. Morales, U.R. Markert Tags: 7th European Congress on Reproductive Immunology Source Type: journals

The pivotal role of protein inhibitors of activated STATs (PIAS) in regulating trophoblastic functionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Signal Transducer and Activator of Transcription 3 (STAT3) is an intracellular signaling molecule which, in trophoblastic cells, can be phosphorylated at its tyr705 (p-STAT3 tyr705) residue by Leukemia Inhibitory Factor (LIF) receptor binding. This phosphorylation correlates with invasiveness. Protein inhibitors of activated STATs (PIAS) antagonize STAT function by interfering with STAT DNA-binding and are, therefore, expected to regulate trophoblast invasion. (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: L. Khachaturyan, T.G. Poehlmann, M. Weber, A.L.L. Forti, D.M. Morales, J.S. Fitzgerald, E. Schleussner, U.R. Markert Tags: 7th European Congress on Reproductive Immunology Source Type: journals

New Approaches to Analyze Follicular FluidsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The analysis of oocytes before fertilization is restricted to morphological aspects. Therefore, we aimed to establish new methods for the analysis of follicular fluids (FF) and granulosa cells (GC). (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: S. Neubeck, I. Hoppe, D. Poehlmann, C. Holzhauer, M. Alunni-Fabbroni, F. von Eggeling, W. Starker, U.R. Markert Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Regulation of Immature Dendritic Cells HIV-1 Infectivity by Uterine Epithelial Cell-Derived Transforming Growth Factor Beta (TGFβ)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mechanisms for sexual transmission of HIV-1 at mucosal sites is poorly defined. Dendritic cells (DC) are critical in the transmission events as either target cells for infection or capture and transfer of HIV-1 to target cells in the female reproductive tract (FRT). An emerging paradigm suggests that the phenotype and immune function of DC is regulated by cytokines and growth factors within distinct tissue sites. These factors may in turn exert effects on the ability of DCs to interact with HIV-1. (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: D.O. Ochiel, M. Ghosh, J.V. Fahey, J.C. Kappes, C. Ochsenbauer-Jambor, P.M. Guyre, C.R. Wira Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Cervical-vaginal lavages from HIV positive- and HIV negative-women contain immune factors that have intrinsic anti-HIV activityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Previous studies from our laboratory have shown that secretions from the human female reproductive tract contain a spectrum of endogenous microbicides, some of which have anti-HIV-1 activity. We hypothesized that cervical-vaginal lavages (CVL) from HIV(+) and HIV(−) women contain endogenous antimicrobials, the levels of which might be dependent on the menstrual cycle. (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: M. Ghosh, J.V. Fahey, Z. Shen, S. Cu-Uvin, K. Mayer, Z. Wu, J. Kappes, C. Ochsenbauer-Jambor, P.F. Wright, C.R. Wira Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Immunohistochemical analysis of STAT3 and its phosphorylated forms in human placentaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Signal transducer and activator of transcription 3 (STAT3) is an intracellular signaling molecule which is involved in several trophoblast functions including invasion, migration and proliferation. STAT3 has one tyrosine and one serine phosphorylation site which display different, not completely known functions. The distribution in normal and pathological human placentas is not yet described. We analyzed 1st (n=10; age 9.3+1.8) and 3rd (n=10; age 33+3.2) trimester human placentae as well as placentae from preeclampsia patients (n=10; age 33.4+1.1). Tissue was fixed in paraformaldehyde and processed for immunoperoxidase sta...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: M. Weber, C. Kuhn, S. Schulze, U. Jeschke, U.R. Markert Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Immunological findings in women miscarrying after LMW heparin therapy. A multicenter studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Laboratory assessment of thrombosis risks in recurrent pregnancy loss (RPL) is not suggested by scientific societies, not only in Greece but also in Europe. In clinical practice, the lack of such guidelines results in preventive LMW heparin administration to women presenting with one or more miscarriages. A remarkable percentage of these women will experience a successful delivery under this treatment, while another percentage will miscarry again. Aim of this retrospective study is to investigate the immunological, immunogenetic and thrombophilic profile of these women, and if possible to propose pregnancy loss preventing ...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: M. Daniilidis, A. Gerofotis, P. Marinaki, A. Vassilaki, V. Dalavitsou, N. Karyotis Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Improved pregnancy rate in IVF patients treated with prophylactic low molecular weight heparin (LMWH)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, there are at present no guidelines for use, or duration, of thromboprophylaxis during AR treatment. These preliminary data support incorporation of trials of thromboprophylaxis into stimulation protocols. (Supported by IGA grant A18621-5.) (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: I. Oborná, J. Březinová, V. Krčová, H. Fingerová, Z. Sobotková, A. Hluší, L. Slavík, J. Úlehlová Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Expansion of MSCs seeded in biological scaffoldsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion a promising approach in the tissue engineering field might be the cellularisation of tissue scaffolds using in vitro repopulation with autologous hMSCs coupled with physically appropriate conditioning prior to implantation. Therefore, in future the basic principle will be seeding of cells onto a matrix scaffold and use of physically interactive bioreactors for the functional differentiation of mesenchymal stem cells within 3-dimensional scaffolds in vitro. (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: S. Michalopoulos, Th. Chatzistamatiou, Ch. Saliagopoulou, A. Thiakos, A. Papassavas, C. Stavropoulos-Giokas Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Study of placentation in PLP-A null, PLP-A/IL-15 null and IL-15 null mutant miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, it is possible that CD11c do not be the intermediates between trophoblast cells and uNK cells, because their number and distribution not altered in different animals. There are pathways for success of pregnancy, and this depend of presence of trophoblast and presence of uNK cells in the environment uterine. The animals’ knockdown has difference of placentation, and the presence of uNK cells can be determinant factor for success of pregnancy. (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: E.M.O. Lippe, S. Alam, M.J. Soares, A.T. Yamada Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Birth defect rates in women using Adalimumab (Humira) and intravenous immunoglobulin to treat immunologic-based infertility in IVFEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study compares the birth defect rate in women using a TNF-α inhibitor (Adalimumab) and/or intravenous immunoglobulin (IVIG) to a similar population of women not using these immunological therapies during an IVF cycle. 156 sub-fertile women aged≤38 years experienced ongoing pregnancies of which 52 resulted in twin pregnancies (208 babies). These successful cycles were divided into three different treatment groups: Group I comprised 31 cycles (23 ICSI) using preconception Adalimumab (Humira) with or without pre or post conception IVIG (last dose of Humira given 67.3±40.8 days before embryo transfer). Group II compri...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: E.E. Winger, J.L. Reed, S. Ashoush, S. Ahuja, J. Jadhav, T. El-Toukhy, M. Taranissi Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Intralipid suppresses NK cytotoxicity both in vitro and in vivo and enhances live birth rates among women experiencing recurrent reproductive failureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To establish the ability of intralipids to suppress NK cytotoxicity both in vitro and in vivo, blood samples taken from 275 women experiencing reproductive failure were analyzed for NK cytotoxicity and the suppression of NK cytotoxicity by intralipid 1:1000 (n=275) using immunofluorescent labeled K562 cells as targets and flow cytometry. 79 patients with abnormal NK activity results (NKa) received Intralipid 20% i.v. (9mg/ml total blood volume—corresponds to 2ml of Intralipid 20% diluted in 250ml saline; or 18mg/ml—corresponds to 4ml of Intralipid 20% diluted in 250ml saline) infusions and their NKa were tested by flow...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: C.Coulam, B. Acacio, R. Roussev Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Diagnostic of infertility—immunological laboratory markersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
How to examine immunological factors of male and female infertility in routine clinical immunological laboratories? Proposed algorithms in clinical reproductive immunology—Male: Child (yes) (in the case of patients with long-term interval from the last delivery follow the childless patients guidelines). Childless: spermiogram 2 times with in 7 days—normal results: MAR test direct-positive-probable cause of infertility→anti sperm Ab in serum, MAR test direct negative→activation markers of NK cells+detection MIF (sperm)+aTTG+anti endomysium Ab+diagnostic of systemic diseases+acrosomal proteins. Abnormal result: agglu...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: M. Drahosova, J. Madar, I. Janatkova, K. Jankovicova Tags: 7th European Congress on Reproductive Immunology Source Type: journals

The frequency distribution of thrombophilic mutations and polymorphisms in women with recurrent miscarriageEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was the evaluation of thrombophilic profile of 242 women with recurrent miscarriage defined as more than two consequent pregnancy losses. Over the last 4 years 427 women with at last one abortion were visited the recurrent miscarriage unit of 1st Obstetric and Gynecologic Clinic of University of Athens. Inclusion criteria for participation in our study were more than two consequent miscarriages and exclusion of the other possible causes of miscarriages (anatomic, endocrine, infectious and autoimmune). 242 women satisfied our criteria and were finally included in our study and we searched in the record...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: P. Drakakis, A. Vlismas, J. Pafilis, D. Loutradis, A. Antsaklis Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Cytokine genes polymorphisms and recurrent pregnancy loss in a Hellenic populationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Aetiology and treatment of immune infertility is highly debated during the last twenty years. Impaired production of T helper type 2 (Th2) as well as higher production of T helper type 1 (Th1) cytokines may support an immunological mechanism for recurrent pregnancy loss (RPL). Immunoregulating and immunogenetic factors may also participate in this process. The aim of the present study was to investigate the relationship between cytokine gene polymorphisms and RPL in women born and living in Northern Greece. We studied 60 childless women of Hellenic origin and nationality, mean age 33±5 years, which experienced at least 3 ...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: A. Vasilaki, M. Daniilidis, A. Gerofotis, M. Tsilipakou, V. Dalavitsou, N. Karyotis, G. Kokaraki Tags: 7th European Congress on Reproductive Immunology Source Type: journals

PIBF polymorphisms in abortionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Progesterone-Induced Blocking Factor (PIBF) is an immunomodulatory molecule, synthesized by lymphocytes of pregnant women. PIBF is implicated in the maintenance of pregnancy, most likely by modifying cytokine balance and inhibiting NK cell activity. The PIBF gene is mapped to chromosome 13q21–q22. A large number of common single nucleotide polymorphisms (SNPs) have been detected within the gene sequence. Some of them have been suggested to modulate NK cell activity (rs1372000) or to correlate with inter-individual variations in actual PIBF production (rs17089760, rs9543118). The aim of this study was to investigate wheth...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: M. Spyropoulou-Vlachou, M. Dinou, Th. Keramitsoglou, P. Arck, M. Rucke, M. Varla-Leftherioti, J. Szekeres-Bartho Tags: 7th European Congress on Reproductive Immunology Source Type: journals

A novel NK cell population with cytoplasmic CD3ɛ expression, is increased in cord blood as well as in peripheral blood of women with recurrent spontaneous abortions (RSA)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It has been shown that a subset of human NK cells shows similarities with T cells. They express Fc epsilon RI gamma (FcɛRIγ) and CD3 zeta chain (CD3ζ) along with other components of the CD3–T-cell receptor (TCR) complex. In particular, it has been reported that fetal and thymic NK cells express intracellular CD3ɛ chain (cyCD3+ NK) raising speculations that these cyCD3+ NK cells are related to thymus-derived NK cells. Besides CD3(−) CD56+CD16+ NK cytotoxic cells (NK1) a subpopulation of less cytotoxic but that are stronger cytokine producing NK cells CD56brightCD16(−) are known and as NK2. While NK1 and NK2 have b...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: F. Gogali, K. Foifa, P. Vasileiou-Pateraki, G. Paterakis Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Phenotypic and functional differences in NK cell cytokine production differed between severe preeclampsia patients and normal pregnant women and support the systemic inflammatory hypothesis in severe preeclampsiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to compare HLA-E and HLA-G receptor expression, Natural Killer cytotoxicity and intracellular cytokine production, freshly isolated peripheral natural killer and mononuclear cells obtained from severe preeclampsia (sPE) patients or gestational age-paired normal pregnant women were analyzed. Ten patients suffering severe preeclampsia (arterial pressure>139/89mmHg and proteinuria>2g/24h urine after 22nd week of gestation), and 10 gestational-age paired pregnant women were selected. Proportion of dim/bright NK cells (CD56+CD16+/ CD56+CD16−) and NKT (CD56+/CD3+), as well as ILT2, KIRDL4, NKG2A, NKG2C receptors were ...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: J.C. Bueno, B. Agudelo, L.F. Escobar, Ch.A. Piedrahita, A. Lopera, A.P. Cadavid, G. Chaouat, J.G. Maldonado-Estrada Tags: 7th European Congress on Reproductive Immunology Source Type: journals

PGE1 versus PGE2: protective rather than inducing agent for endometriosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The ectopical implantation of endometrium leading to endometriosis has been correlated to various immune as well as non-immune parameters. Thus, inflammatory cytokines including IL-2, IFN-γ, TNF-α, IL-4 have been found to be increased in serum and peritoneal fluid of endometriotic patients at the same time that the elevated levels of prostaglandins PGE1 and PGE2 have been accused to facilitate the inflammation process. Using the experimental model of l-carnitine (l-Cn)-induced endometriosis, the aim of the present study was to explore the role of PGE1 and PGE2 in endometriosis development. During the treatment for endome...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: A. Tselekidou, I. Athanassakis Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Anti-fibrillin-1 autontibodies in normal pregnancy and recurrent pregnancy lossEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Fibrillin-1 is an extracellular matrix glycoprotein, a main component of microfibrils as free bundles or in association with elastin-containing elastic fibrils. It is present in the structures undergoing intensive remodeling during menstrual cycle and pregnancy, hence endometrium and decidua. The aim of this study was to investigate anti-fibrillin-1 autoantibodies in patients with a history of recurrent pregnancy loss (RPL) and during normal pregnancy. Anti-fibrillin-1 IgG and IgM autoantibodies were measured by a home-made ELISA in serum samples of 48 medically and obstetrically normal pregnant women, classified to three ...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: M. Atanasova, E. Konova, T. Aleksovska, K. Todorova, T. Lukanov Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Soluble CD30 (sCD30) in normal pregnancy, pre-eclampsia and recurrent spontaneous abortion (RSA)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study at first the sCD30 level have been checked in sera of a group of normal non-pregnant women (N=40) and compared with normal pregnancy at the first (N=30) and third (N=30) trimester. Furthermore, the level of sCD30 in the normal first and third trimester pregnancy were compared with RSA (N=30) and pre-eclamptic (N=30) patients respectively. The level of sCD30 before and after leukocyte therapy was also checked in a group of RSA (N=15) patients. sCD30 levels were checked by ELISA method and non-parametric Mann–Whitney test or T-test was used for statistical analysis. The mean level of sCD30 at the first trimes...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: B. Gharesi-Fard, J. Zolghadri, F. Tavazoo Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Systemic Th1- and Th2-associated chemokines during and after pregnancy in relation to maternal allergic diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Prenatal exposure to a strong Th2-like environment could favour the development of Th2-like immune responses in the offspring. We have previously observed increased total IgE levels at gestational week 10 compared to 1 year after delivery in allergic, but not non-allergic, women, indicating that allergy is associated with a more pronounced Th2 deviation during pregnancy. The aim of this study is to characterize the systemic Th1- and Th2-associated chemokine levels during pregnancy and after delivery among allergic and non-allergic women. The chemokine levels were determined by a Luminex assay at five occasions during pregn...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: M. Sandberg, J. Ernerudh, G. Berg, L. Matthiesen, C. Ekerfelt, L.J. Nilsson, M.C. Jenmalm Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Immunolocalization of pro- and anti-apoptotic molecules in early human placentas in abortion and healthy pregnanciesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The study was performed to determine whether abnormal trophoblast apoptosis is associated with spontaneous abortion. Placental and endometrial specimens from patients with spontaneous abortion (n=18); women undergoing elective abortion (n=3) and nonpregnant women (n=3) were analyzed. Bcl-2, Bcl-xs, Bag-1, Bax, expressions were assessed by immunohistochemistry and apoptosis by the TUNEL (TdT-mediated dUTP nick end labeling) method. Pro-apoptotic and anti-apoptotic molecules were expressed in all specimens. TUNEL and Bax positive cells were much more prominent in spontaneous abortion specimens than in normal placentas. On th...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: S. Daher, A.J. Guimarães, R. Mattar, A.C. Zenclussen, L.R. Paulesu, E. Bevilacqua Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Evaluation of thyroglobulin expression in murine reproductive organs during estrous cycle and pregnancyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In pregnant women with anti-thyroglobulin antibody (aTG), prevalence of abortion is 2–4 fold higher compared to normal control. Although thyroid dysfunction may be the case in these settings, direct effect of such harmful autoantibodies on female reproductive organs may serve a role in pregnancy loss. To address this hypothesis, in this study, expression of thyroglobulin gene and protein in reproductive organs of Balb/c mice during estrous cycle and pregnancy have been evaluated. Stages of estrous cycle were determined by examining vaginal cytology. Pregnancy (Balb/c×Balb/c and Balb/c×C57BL/6) was confirmed by presence...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: A.H. Zarnani, A. Moravvej, A. Kalantari, R. Ghods, G.E. Kazemi-Sefat, J. Ghasemi, M.A. Akhondi, M. Jeddi-Tehrani Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Pregnancy-induced hypertension (PIH) is accompanied with a decreased number of circulating endothelial progenitor cells (EPCs)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to determine whether the EPCs count in the peripheral blood of pregnant women with pregnancy-induced hypertension (PIH) is different from that found in the peripheral blood of healthy pregnant women. The study included 18 pregnant women with pregnancy-induced hypertension (PIH). The control group consisted of 21 pregnant women with normal blood pressure levels. Venous blood samples were collected into test tubes prepared with sodium heparin before labour. Mononuclear peripheral blood cells were isolated and marked with the help of monoclonal antibodies particular to the markers of endothelia...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: J. Heimrath, A. Czekański, M. Paprocka, A. Ledwożyw, D. Duś Tags: 7th European Congress on Reproductive Immunology Source Type: journals

The protective roles of non-classical MHC class Ib HLA-G antigen against maternal macrophagesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human leukocyte antigen (HLA)-G is an immune-suppressive major histocompatibility complex class Ib molecule. It is expressed by fetal trophoblasts cells of the placenta. it has been postulated that it plays a role in protecting the fetus by acting as inhibitory receptors of natural killer cells and CD8+ T cells. It is still unclear how and whether HLA-G antigen can protect the trophoblast against activated macrophages. The aim of this study is to investigate whether HLA-G can alter macrophages responses when they come in close contact with trophoblast-like cell lines. We wish to evaluate the interactions between HLA-G expr...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: A. Shakhawat, V. Shaikly, E. Mavrakos, N. Fernández Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Lymphocyte subsets during normal pregnancyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, the main differences between non-pregnant and pregnant women at any trimester appear to concern the total T lymphocyte population and partially the CD8+ T cells. Serial testing in pregnant women during the 3 trimesters would provide more information. (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: A. Asimomytis, C. Tsekoura, G. Perros, E. Kalarhaki, P. Tzonis, D. Papaioannou, C. Balafoutas, M. Varla-Leftherioti Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Immunophenotype of dendritic cells in renal transplanted pregnant women at first, second and third trimesterEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Introduction and objective: Immunological tolerance is a key for successful maternal–fetal interaction and transplantation. Dendritic cells (DCs) are critically involved in the starting of primary immune response and also in the induction of immunological tolerance and maintenance of immune homeostasis. Pregnancy after solid organ transplantation is no longer uncommon, especially in the view of the recent advances in surgery and immunosuppressant. In this context, the aim of this study is to characterize dendritic cells in pregnant women after renal transplantation. Methods: The whole blood was obtained from renal transp...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: S. Correa-Silva, L.G. Oliveira, N. Sass, N.O. Camara, E. Bevilacqua Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Systemic reduction of functionally suppressive CD4dimCD25highFoxp3+ Tregs in human second trimester pregnancy is induced by progesterone and 17β-estradiolEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CD4+CD25high regulatory T cells (Tregs) are implicated in maintenance of murine pregnancy but reports regarding circulating Tregs frequencies in human pregnancy are inconsistent. The aim was to determine the frequency, phenotype and function of circulating Tregs in human pregnancy and the influence of progesterone and 17β-estradiol on Tregs phenotype and frequency. 38-second trimester pregnant and 65 non-pregnant women participated in the study. Freshly isolated or in vitro progesterone and 17β-estradiol stimulated PBMCs were analysed for expression of CD25, Foxp3, CD127 and HLA-DR using flow cytometry. FACSAria sorted C...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: J. Mjösberg, J. Svensson, E. Johansson, L. Hellström, R. Casas, M.C. Jenmalm, R. Boij, L. Matthiesen, J.-I. Jönsson, G. Berg, J. Ernerudh Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Overexpression of NKp46 IN CD3−CD56+ NK cells in couples with unexplained recurrent spontaneous abortions (URSA)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, women with URSA in comparison to their partners showed increased incidence expression of natural cytotoxicity receptor NKp46 and decreased incidence of inhibitory CD158a and CD158b receptors. These disturbances in couples with URSA concern mainly CD3−CD56+ NK cells subset. Further study of the interaction between NK cells receptors and respective HLA antigens in each couple could possibly clarify the role of these receptors in URSA. (Source: Journal of Reproductive Immunology)
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: E. Konsta, K. Psarra, V. Kapsimali, V. Kitsiou, G. Galaziou, A. Katsavria, S. Dendrinos, G. Creatsas, C. Papasteriades Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Preimplantation Factor (PIF*): non-invasive biomarker for embryo selection in IVFEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Preimplantation Factor (PIF) is an embryo-derived peptide, which is detected during viable pregnancy in human and other mammals. The aim of the present study was to investigate whether PIF levels in embryo culture supernatants of women undergoing IVF is associated with the IVF outcome, and to evaluate if PIF measurements can be used as a biomarker to identify viable embryos and increase implantation/pregnancy rates. Supernatant samples from 139 single embryo cultures from 45 women undergoing IVF, were analyzed. Samples were collected on day 2 or 3 post-fertilization. To each woman, 2–3 morphologically good embryos were t...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: E. Promponas, T. Keramitsoglou, S. Ramu, C.W. Stamatkin, K. Pantos, T. Vaxevanoglou, S. Koussoulakos, J. Diakakis, M. Varla-Leftherioti, E.R. Barnea Tags: 7th European Congress on Reproductive Immunology Source Type: journals

G-CSF pharmacologic supplementation in the ART (Assisted Reproductive Technologies) treatment cycles of low responder womenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Granulocyte-Colony Stimulating Factor (G-CSF) is a cytokine discovered as a growth factor for granulocytes but it has been shown to have a lot of other physiologic actions. Recently, its role in the reproductive systems has been pointed out and particularly in ovarian follicles where the G-CSF concentration was found to be directly correlated to the oocyte quality and this was in relationship to the patient age. In all the techniques of ART (Assisted Reproductive Technology) oocyte number recruitment is crucial and a good quality of them is mandatory for the success of the event. The most important problem for every ART pr...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: F. Scarpellini, M. Sbracia, A. Patella Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Cellular immunity in the outcome of in vitro fertilization–embryo transferEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The close relationship of reproductive outcome and immune system has been recognized many years ago and has been extensively studied. In vitro fertilization (IVF) and embryo transfer (ET) are well-established techniques overcoming the problem of infertility. The rate of IVF/ET success remains low, with an elusive etiology in most cases. The aim of this study was to evaluate the involvement of cellular immunity in women with multiple failures of IVF/ET attempts, through the assessment of peripheral blood (PB) leukocyte subpopulations. The study included 55 infertile women with failed IVF treatments and 45 fertile women used...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: V. Kitsiou, S. Dendrinos, K. Psarra, V. Kapsimali, G. Creatsas, C. Papasteriades Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Psychological stress, immune system and IVF outcomeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Psychological distress has become a commonly sited factor when discussing reproductive failures. Psychological stress may act through different plausible mechanisms on the reproductive system. Three systems – the endocrine, immune and nervous systems – engage in multiple interactions with the body's response to acute and chronic stress. The hypothalamic-pituitary-adrenal (HPA) axis, when activated by stress, exerts an inhibitory effect on the female reproductive system. Corticotropin-releasing hormone inhibits GnRH secretion, and glucocorticoids inhibit LH and ovarian estrogen and progesterone secretion. An acute stres...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: K. Gourounti, V. Kapetanios, N. Paparisteidis, G. Vaslamatzis, F. Anagnostopoulos Tags: 7th European Congress on Reproductive Immunology Source Type: journals

Markers associated with success and failure of embryo transfer (ET) during IVF programEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In terms of IVF-ET program we have investigated cytokines mRNA expression profiles and bacterial contamination background in endometrial tissue as well as vaginal flora content by means of qPCR. The subjects were women (n=23) with tubo-peritoneal factor of infertility. The following cytokines were analyzed in the study with normalization to “housekeeping genes” (HPRT1, TBP, B2M) using ΔΔCp method: IL-1β, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12a/p35, IL-12b/IL-23/p40, IL-15, IL-17, IL-18, IL-23/p19, TNF-α, TGF-β, IFN-γ, Foxp3, LIF, and α-chain IL-2R. The quantity of total bacteria, Lactobacillus spp. and 14 ma...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: D.Yu. Trofimov, O.V. Burmenskaya, M.V. Ebzeeva, E.A. Kalinina, S.M. Mullabaeva, L.N. Kuzjmichev, G.T. Sukhikh Tags: 7th European Congress on Reproductive Immunology Source Type: journals

FACS analysis of NK cells in IVF-treated patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In vitro fertilization with subsequent transfer of the embryo (IVF-ET) still proves a low implantation rate (slightly over 30%). The attention of investigators therefore switches from the technique of IVF towards the questions of embryo implantation. It follows from the very nature of implantation that the crucial factor of materno-fetal interaction is the immunity. Approximately 70% of lymphocytes residing in the pregnant decidua are NK cells. These are phenotypically distinct from peripheral blood NK cells in expression of extremely high levels of CD56. We analyzed samples of peripheral blood and follicular fluid of pati...
Source: Journal of Reproductive Immunology - August 18, 2009 Category: Allergy & Immunology Authors: J. Madar, J. Svoboda, Z. Ruzickova, L. Cuchalova, J. Rezacova, A. Fiserova Tags: 7th European Congress on Reproductive Immunology Source Type: journals