Heparin-mediated PCR interference in SARS-CoV-2 assays and subsequent reversal with heparinase I
This study aimed to investigate the magnitude of heparin interference across various clinical laboratory testing platforms, and the reversal of any interference by degradation of heparin using the heparinase I enzyme in nasopharyngeal swab (NP) samples for SARS-CoV-2 analysis by RT-qPCR. Heparin-mediated PCR interference was evident at heparin concentrations as low as 10 IU/mL across all platforms tested, with the exclusion of the Hologic Panther Aptima SARS-CoV-2 assay. Rates of false negative or invalid results and falsely elevated cycle threshold (Ct) values increased with increasing heparin concentrations on all platfo...
Source: Journal of Virological Methods - April 22, 2024 Category: Virology Authors: K Edwards T Corocher Y Hersusianto D Campbell K Subbarao J A Neil P Monagle P Ho Source Type: research
Validation of a severe acute respiratory syndrome coronavirus 2 microneutralization assay for evaluation of vaccine immunogenicity
J Virol Methods. 2024 Apr 20:114945. doi: 10.1016/j.jviromet.2024.114945. Online ahead of print.ABSTRACTAs variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continue to emerge, assessment of vaccine immunogenicity remains a critical factor to support continued vaccination. To this end, an in vitro microneutralization (MN50) assay was validated to quantitate SARS-CoV-2 neutralizing antibodies against prototype and variant strains (Beta, Delta, Omicron BA.1, Omicron BA.5, and XBB.1.5) in human serum. For the prototype strain, the MN50 assay met acceptance criteria for inter-/intra-assay precision, spec...
Source: Journal of Virological Methods - April 22, 2024 Category: Virology Authors: Stephanie Hamilton Mingzhu Zhu Shane Cloney Clark Penny Mayes Jen Fenner Leah Cui Rongman Cai Raj Kalkeri Louis F Fries Melinda Pryor Joyce Plested Source Type: research
Real-world performance of the NeuMoDx ™ HCV Quant Test for quantification of hepatitis C virus (HCV)-RNA
J Virol Methods. 2024 Apr 11;327:114937. doi: 10.1016/j.jviromet.2024.114937. Online ahead of print.ABSTRACTQuantification of hepatitis C virus (HCV)-RNA in serum or plasma samples is an essential parameter in HCV diagnostics. Here, the NeuMoDx™Molecular System (Qiagen) was tested for the most common HCV genotypes and compared to the cobas c6800 system (Roche). HCV-RNA from 131 plasma/serum samples from chronically infected patients was determined in parallel on the NeuMoDx and c6800 systems. Linearity was analysed using the four most common HCV genotypes (1-4) in our cohort. The coefficient of variation (CV) within (int...
Source: Journal of Virological Methods - April 13, 2024 Category: Virology Authors: Nadine L übke Andreas Walker Martin Obermeier Jennifer Camdereli Martha Paluschinski Lara Walotka Anna-Kathrin Schupp Inga Tometten Sandra Hauka Eva Heger J örg Timm Source Type: research
Real-world performance of the NeuMoDx ™ HCV Quant Test for quantification of hepatitis C virus (HCV)-RNA
J Virol Methods. 2024 Apr 11:114937. doi: 10.1016/j.jviromet.2024.114937. Online ahead of print.ABSTRACTQuantification of hepatitis C virus (HCV)-RNA in serum or plasma samples is an essential parameter in HCV diagnostics. Here, the NeuMoDx™Molecular System (Qiagen) was tested for the most common HCV genotypes and compared to the cobas c6800 system (Roche). HCV-RNA from 131 plasma/serum samples from chronically infected patients was determined in parallel on the NeuMoDx and c6800 systems. Linearity was analysed using the four most common HCV genotypes (1-4) in our cohort. The coefficient of variation (CV) within (intra-a...
Source: Journal of Virological Methods - April 13, 2024 Category: Virology Authors: Nadine L übke Andreas Walker Martin Obermeier Jennifer Camdereli Martha Paluschinski Lara Walotka Anna-Kathrin Schupp Inga Tometten Sandra Hauka Eva Heger J örg Timm Source Type: research
Real-world performance of the NeuMoDx ™ HCV Quant Test for quantification of hepatitis C virus (HCV)-RNA
J Virol Methods. 2024 Apr 11:114937. doi: 10.1016/j.jviromet.2024.114937. Online ahead of print.ABSTRACTQuantification of hepatitis C virus (HCV)-RNA in serum or plasma samples is an essential parameter in HCV diagnostics. Here, the NeuMoDx™Molecular System (Qiagen) was tested for the most common HCV genotypes and compared to the cobas c6800 system (Roche). HCV-RNA from 131 plasma/serum samples from chronically infected patients was determined in parallel on the NeuMoDx and c6800 systems. Linearity was analysed using the four most common HCV genotypes (1-4) in our cohort. The coefficient of variation (CV) within (intra-a...
Source: Journal of Virological Methods - April 13, 2024 Category: Virology Authors: Nadine L übke Andreas Walker Martin Obermeier Jennifer Camdereli Martha Paluschinski Lara Walotka Anna-Kathrin Schupp Inga Tometten Sandra Hauka Eva Heger J örg Timm Source Type: research
Real-world performance of the NeuMoDx ™ HCV Quant Test for quantification of hepatitis C virus (HCV)-RNA
J Virol Methods. 2024 Apr 11:114937. doi: 10.1016/j.jviromet.2024.114937. Online ahead of print.ABSTRACTQuantification of hepatitis C virus (HCV)-RNA in serum or plasma samples is an essential parameter in HCV diagnostics. Here, the NeuMoDx™Molecular System (Qiagen) was tested for the most common HCV genotypes and compared to the cobas c6800 system (Roche). HCV-RNA from 131 plasma/serum samples from chronically infected patients was determined in parallel on the NeuMoDx and c6800 systems. Linearity was analysed using the four most common HCV genotypes (1-4) in our cohort. The coefficient of variation (CV) within (intra-a...
Source: Journal of Virological Methods - April 13, 2024 Category: Virology Authors: Nadine L übke Andreas Walker Martin Obermeier Jennifer Camdereli Martha Paluschinski Lara Walotka Anna-Kathrin Schupp Inga Tometten Sandra Hauka Eva Heger J örg Timm Source Type: research
Real-world performance of the NeuMoDx ™ HCV Quant Test for quantification of hepatitis C virus (HCV)-RNA
J Virol Methods. 2024 Apr 11:114937. doi: 10.1016/j.jviromet.2024.114937. Online ahead of print.ABSTRACTQuantification of hepatitis C virus (HCV)-RNA in serum or plasma samples is an essential parameter in HCV diagnostics. Here, the NeuMoDx™Molecular System (Qiagen) was tested for the most common HCV genotypes and compared to the cobas c6800 system (Roche). HCV-RNA from 131 plasma/serum samples from chronically infected patients was determined in parallel on the NeuMoDx and c6800 systems. Linearity was analysed using the four most common HCV genotypes (1-4) in our cohort. The coefficient of variation (CV) within (intra-a...
Source: Journal of Virological Methods - April 13, 2024 Category: Virology Authors: Nadine L übke Andreas Walker Martin Obermeier Jennifer Camdereli Martha Paluschinski Lara Walotka Anna-Kathrin Schupp Inga Tometten Sandra Hauka Eva Heger J örg Timm Source Type: research
Clinical validation of SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex
CONCLUSIONS: It demonstrates the potential of electrochemical biosensors to be implemented as highly sensitive and easily deployable detection strategies even in remote locations.PMID:38608761 | DOI:10.1016/j.jviromet.2024.114940 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - April 12, 2024 Category: Virology Authors: Viviana V ásquez Jahir Orozco Source Type: research
A Validated In-House Assay for HIV Drug Resistance Mutation Surveillance from Dried Blood Spot Specimens
J Virol Methods. 2024 Apr 9:114939. doi: 10.1016/j.jviromet.2024.114939. Online ahead of print.ABSTRACTDespite increasing scale-up of antiretroviral therapy (ART) coverage, challenges related to adherence and HIV drug resistance (HIVDR) remain. The high cost of HIVDR surveillance is a persistent challenge with implementation in resource-constrained settings. Dried blood spot (DBS) specimens have been demonstrated to be a feasible alternative to plasma or serum for HIVDR genotyping and are more suitable for lower resource settings. There is a need for affordable HIVDR genotyping assays which can amplify HIV-1 sequences from...
Source: Journal of Virological Methods - April 11, 2024 Category: Virology Authors: Bronwyn Neufeld Chantal Munyuza Alexandria Reimer Rupert Capi ña Emma R Lee Marissa Becker Paul Sandstrom Hezhao Ji Fran çois Cholette Source Type: research
A Validated In-House Assay for HIV Drug Resistance Mutation Surveillance from Dried Blood Spot Specimens
J Virol Methods. 2024 Apr 9:114939. doi: 10.1016/j.jviromet.2024.114939. Online ahead of print.ABSTRACTDespite increasing scale-up of antiretroviral therapy (ART) coverage, challenges related to adherence and HIV drug resistance (HIVDR) remain. The high cost of HIVDR surveillance is a persistent challenge with implementation in resource-constrained settings. Dried blood spot (DBS) specimens have been demonstrated to be a feasible alternative to plasma or serum for HIVDR genotyping and are more suitable for lower resource settings. There is a need for affordable HIVDR genotyping assays which can amplify HIV-1 sequences from...
Source: Journal of Virological Methods - April 11, 2024 Category: Virology Authors: Bronwyn Neufeld Chantal Munyuza Alexandria Reimer Rupert Capi ña Emma R Lee Marissa Becker Paul Sandstrom Hezhao Ji Fran çois Cholette Source Type: research
Development and analytical validation of a novel quantitative PCR assay for the detection of Trachemys herpesvirus 1
This study aimed to develop a sensitive and specific quantitative real-time PCR (qPCR) assay for the detection of TrHV1 DNA to aid in the characterization of the epidemiology of this virus in aquatic turtles. Two TaqMan-MGB FAM-dye labeled primer-probe sets were designed and evaluated using plasmid dilutions. The higher performing assay was specific for TrHV1 DNA and had a linear dynamic range of 1.0 × 107 to 1.0 × 101 copies per reaction with an R2 of 0.999, slope of -3.386, and efficiency of 97.39%. The limit of detection was 101 copies per reaction, and there was no loss of reaction efficiency in the presence of TrHV1...
Source: Journal of Virological Methods - April 10, 2024 Category: Virology Authors: Kaitlin A Moorhead Laura A Adamovicz Matthew C Allender Source Type: research
Development and analytical validation of a novel quantitative PCR assay for the detection of Trachemys herpesvirus 1
This study aimed to develop a sensitive and specific quantitative real-time PCR (qPCR) assay for the detection of TrHV1 DNA to aid in the characterization of the epidemiology of this virus in aquatic turtles. Two TaqMan-MGB FAM-dye labeled primer-probe sets were designed and evaluated using plasmid dilutions. The higher performing assay was specific for TrHV1 DNA and had a linear dynamic range of 1.0 × 107 to 1.0 × 101 copies per reaction with an R2 of 0.999, slope of -3.386, and efficiency of 97.39%. The limit of detection was 101 copies per reaction, and there was no loss of reaction efficiency in the presence of TrHV1...
Source: Journal of Virological Methods - April 10, 2024 Category: Virology Authors: Kaitlin A Moorhead Laura A Adamovicz Matthew C Allender Source Type: research
RSV-GenoScan: an automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis
We present RSV-GenoScan, a fast and easy-to-use pipeline for WGS analysis of RSV generated by HTS on Illumina or Nanopore platforms. RSV-GenoScan automates the WGS analysis steps directly from the raw sequence data. The pipeline filters the sequence data, maps the reads to the RSV reference genome, generates a consensus sequence, identifies the RSV subgroup, and lists amino acid mutations, insertions and deletions in the F and G viral genes. This enables the rapid identification of mutations in these coding genes that are known to confer resistance to monoclonal antibodies.AVAILABILITY: RSV-GenoScan is freely available at ...
Source: Journal of Virological Methods - April 8, 2024 Category: Virology Authors: Alexandre Dosbaa Romane Guilbaud Anna-Maria Franco Yusti Valentine Marie Ferr é Charlotte Charpentier Diane Descamps Quentin Le Hingrat Romain Copp ée Source Type: research
RSV-GenoScan: an automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis
We present RSV-GenoScan, a fast and easy-to-use pipeline for WGS analysis of RSV generated by HTS on Illumina or Nanopore platforms. RSV-GenoScan automates the WGS analysis steps directly from the raw sequence data. The pipeline filters the sequence data, maps the reads to the RSV reference genome, generates a consensus sequence, identifies the RSV subgroup, and lists amino acid mutations, insertions and deletions in the F and G viral genes. This enables the rapid identification of mutations in these coding genes that are known to confer resistance to monoclonal antibodies.AVAILABILITY: RSV-GenoScan is freely available at ...
Source: Journal of Virological Methods - April 8, 2024 Category: Virology Authors: Alexandre Dosbaa Romane Guilbaud Anna-Maria Franco Yusti Valentine Marie Ferr é Charlotte Charpentier Diane Descamps Quentin Le Hingrat Romain Copp ée Source Type: research
RSV-GenoScan: an automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis
We present RSV-GenoScan, a fast and easy-to-use pipeline for WGS analysis of RSV generated by HTS on Illumina or Nanopore platforms. RSV-GenoScan automates the WGS analysis steps directly from the raw sequence data. The pipeline filters the sequence data, maps the reads to the RSV reference genome, generates a consensus sequence, identifies the RSV subgroup, and lists amino acid mutations, insertions and deletions in the F and G viral genes. This enables the rapid identification of mutations in these coding genes that are known to confer resistance to monoclonal antibodies.AVAILABILITY: RSV-GenoScan is freely available at ...
Source: Journal of Virological Methods - April 8, 2024 Category: Virology Authors: Alexandre Dosbaa Romane Guilbaud Anna-Maria Franco Yusti Valentine Marie Ferr é Charlotte Charpentier Diane Descamps Quentin Le Hingrat Romain Copp ée Source Type: research
