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Specific detection of Arcobacter spp. in estuarine waters of Southern Italy by PCR and fluorescent in situ hybridizationemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Culturable and nonculturable forms of Arcobacter in the estuarine environment were present. PCR assays were more sensitive than traditional culture in detecting Arcobacter butzleri and A. cryaerophilus. FISH comparatively to PCR technique may provide information about cell morphology and viability of single cells.Significance and Impact of the Study: Our investigation indicates the existence of an environmental reservoir of potential pathogenic arcobacters in an estuarine Italian area, which may survive under a viable but not culturable state. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - November 20, 2009 Category: Microbiology Authors: M.T. Fera, C. Gugliandolo, V. Lentini, A. Favaloro, D. Bonanno, E. La Camera, T.L. Maugeri Source Type: journals

Molecular cloning and transcriptional analysis of a newly identified anti-lipopolysaccharide factor gene in kuruma shrimp, Marsupenaeus japonicusemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The present work revealed the presence of MjALF2 gene, which is highly expressed in gills and muscle of healthy kuruma shrimp. Further studies are required to clarify the involvement of MjALF2 in immune responses for using as a therapeutic agent.Significance and Impact of the Study: Antimicrobial peptides are promising potential therapeutic agents for disease control in aquaculture. Understanding the relation of MjALF2 with innate immunity mechanism will lead to develop better health management strategies for long-term sustainability of the shrimp industry. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - November 14, 2009 Category: Microbiology Authors: T. Mekata, R. Sudhakaran, S. Okugawa, T. Kono, M. Sakai, T. Itami Source Type: journals

Characterization of monooxygenase gene diversity in benzene-amended soilsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The primer set designed in this study successfully detected diverse benzene monooxygenase genes. The level of diversity may have increased because the degradation of benzene differed from soil to soil. Microarrays have great potential in the comprehensive detection of gene richness as well as the elucidation of key genes for degradation.Significance and Impact of the Study: This study introduces a new primer set that may be used to identify diverse benzene monooxygenase genes in the environment; moreover, it demonstrates the potential of microarray technology in the profiling of environmental samples. (Source:...
Source: Letters in Applied Microbiology - November 12, 2009 Category: Microbiology Authors: S. Iwai, F. Kurisu, H. Urakawa, O. Yagi, H. Furumai Source Type: journals

Analysis of the effect of copper on the virulence of a pathogenic Edwardsiella tarda strainemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Prolonged exposure to copper has multiple effects on TX5 and results in significant attenuation of bacterial virulence.Significance and Impact of the Study: The results of this study demonstrate that copper treatment has a broad and profound effect on the virulence-associated capacities of TX5, which is exerted at least in part at the transcription level. These findings provide new insights to the antimicrobial mechanism of copper. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - November 11, 2009 Category: Microbiology Authors: Y.-H. Hu, W. Dang, C.-S. Liu, L. Sun Source Type: journals

Combination of ARAD microfibre filtration and LAMP methodology for simple, rapid and cost-effective detection of human pathogenic Giardia duodenalis and Cryptosporidium spp. in drinking wateremail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The described protozoa detection methodology is sensitive, rapid and cost-effective.Significance and Impact of the Study: This effective procedure will be useful for small waterworks to achieve continuous monitoring and is also of value for screening catchments to identify those that require further treatment and more detailed microscopic counts. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - November 6, 2009 Category: Microbiology Authors: J. Plutzer, A. Törökné, P. Karanis Source Type: journals

Aspergillus fumigatus toxicity and gliotoxin levels in feedstuff for domestic animals and pets in Argentinaemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Feed samples contaminated with gliotoxin are potentially toxic to animals.Significance and Impact of the Study: The presence of gliotoxin could affect animal productivity and health. Moreover, there are risks of contamination to farm workers handling improperly stored animal feed. Aspergillus fumigatus strains isolated from different sources should be investigated to determine prevention and control strategies. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - November 2, 2009 Category: Microbiology Authors: G.A. Pena, C.M. Pereyra, M.R. Armando, S.M. Chiacchiera, C.E. Magnoli, J.L. Orlando, A.M. Dalcero, C.A.R. Rosa, L.R. Cavaglieri Source Type: journals

Development of a minimal growth medium for Lactobacillus plantarumemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: Largely reduced folate production pools do not have an effect on the growth of L. plantarum, showing that L. plantarum makes folate in a large excess.Significance and Impact of the study: These experiments illustrate the importance of combining genome-scale metabolic models with growth experiments on minimal media. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 30, 2009 Category: Microbiology Authors: A. Wegkamp, B. Teusink, W.M. de Vos, E.J. Smid Source Type: journals

Cellular fatty acids as chemical markers for differentiation of Yersinia pestis and Yersinia pseudotuberculosisemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Yersinia pestis could be easily differentiated from Y. pseudotuberculosis by plotting the ratios of some CFA pairs, i.e.,14:0/18:0 vs 18:1[omega]7c/18:0, 3-OH-14:0/18:0 vs 18:1[omega]7c/18:0, 16:1[omega]7c/18:0 vs 18:1[omega]7c/18:0, 12:0/18:0 vs 18:1[omega]7c/18:0 and 12:0 ALDE/18:0 vs 16:1[omega]7c/18:0 fatty acids.Significance and Impact of the Study: In the present study, the normalized Sherlock MIS and Sherlock standard libraries were used to analyse the fatty acid composition of different strains of Y. pestis and Y. pseudotuberculosis. Meanwhile, ratios of certain CFA components were found to serve as ch...
Source: Letters in Applied Microbiology - October 26, 2009 Category: Microbiology Authors: Y. Tan, M. Wu, H. Liu, X. Dong, Z. Guo, Z. Song, Y. Li, Y. Cui, Y. Song, Z. Du, R. Yang Source Type: journals

Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimpemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Bacillus subtilis inhibited Vibrio growth, and both B. licheniformis and B. megaterium suppressed haemolytic activity in Vibrio.Significance and Impact of the Study: The cell-free supernatants produced by Bacillus probionts inhibit Vibrio disease, and Bacillus probionts might have an influence on Vibrio cell-to-cell communications. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 26, 2009 Category: Microbiology Authors: T. Nakayama, H. Lu, N. Nomura Source Type: journals

The zymocidial activity of Tetrapisispora phaffii in the control of Hanseniaspora uvarum during the early stages of winemakingemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Tetrapisispora phaffii is an excellent candidate for the biological control of undesired proliferation of apiculate yeasts during the first steps of fermentation.Significance and Impact of the Study: Tetrapisispora phaffii cells in an immobilized form can be used as a biocontrol agent to reduce the need for SO2 addition. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 23, 2009 Category: Microbiology Authors: F. Comitini, M. Ciani Source Type: journals

Preservation of probiotic strains isolated from kefir by spray dryingemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Spray drying is a suitable method to preserve micro-organisms isolated from kefir grains. A high proportion of cells were still viable after 80 days of storage at refrigerated temperaturesSignificance and Impact of Study: It is the first report about spray-dried probiotic strains isolated from kefir grain and contributes to the knowledge about these micro-organisms for their future application in novel dehydrated products. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 20, 2009 Category: Microbiology Authors: M.A. Golowczyc, J. Silva, A.G. Abraham, G.L. De Antoni, P. Teixeira Source Type: journals

Keratinolytic activity of Bacillus subtilis AMR using human hairemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: This study showed that B. subtilis AMR was able to hydrolyse human hair producing serine peptidases with keratinase and gelatinase activity as well as hair keratin peptides.Significance and Impact of the Study: This is the first report describing the production and partial characterization of keratinases by a B. subtilis strain grown in a medium containing human hair. These data suggest that peptides obtained from enzymatic hair hydrolysis may be useful for future applications on pharmaceutical and cosmetic formulations. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 20, 2009 Category: Microbiology Authors: A.M. Mazotto, S.M. Lage Cedrola, U. Lins, A.S. Rosado, K.T. Silva, J.Q. Chaves, L. Rabinovitch, R.B. Zingali, A.B. Vermelho Source Type: journals

The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3–18email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: One out of 28 analysed Lact. plantarum strains harbours the prtP-like gene. The other proteolytically active Lact. plantarum probably possesses a different type of extracellular proteinase(s).Significance and Impact of the Study: It is the first report about the presence of the prtP[ndash]like gene in Lact. plantarum, which illustrates the mobility of this gene and its presence in different species. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 18, 2009 Category: Microbiology Authors: I. Strahinic, M. Kojic, M. Tolinacki, D. Fira, L. Topisirovic Source Type: journals

Persistence of Escherichia coli O157:H7 on the rhizosphere and phyllosphere of lettuceemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: Escherichia coli O157:H7 survived throughout the growth period as was determined by real-time PCR and by subsequent enrichment and immunomagnetic separation of edible part of plants.Significance and impact of the Study: The potential presence of human pathogens in vegetables grown in soils contaminated with E. coli O157:H7 is a serious problem to our national food supply as the pathogen may survive on the leaf surface as they come in contact with contaminated soil during germination. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 15, 2009 Category: Microbiology Authors: A. Mark Ibekwe, C.M. Grieve, S.K. Papiernik, C.-H. Yang Source Type: journals

Occurrence of Escherichia coli O157:H7 in faeces, skin and carcasses from sheep and goats in Ethiopiaemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Even though the numbers of samples examined in this study were limited to one abattoir, sheep and goats can be potential sources of E. coli O157:H7 for human infection in the country. Control measures to reduce the public health risks arising from E. coli O157:H7 in reservoir animals need to be addressed at abattoir levels by reducing skin and faecal sources and carcass contaminations at different stages of slaughter operations.Significance and Impact of the Study: Escherichia coli O157:H7 was detected from carcasses before and after washing during slaughtering operations, and one O157 isolate was positive for...
Source: Letters in Applied Microbiology - October 15, 2009 Category: Microbiology Authors: G. Mersha, D. Asrat, B.M. Zewde, M. Kyule Source Type: journals

Effects of feeding and induction strategy on the production of BmR1 antigen in recombinant E. coliemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Combination of feeding at a lower [mu]set and twice induction with 1 mmol l[minus]1 IPTG yielded the best result of all variables tested, promising an improved method for BmR1 production.Significance and Impact of the Study: This method can be used to increase the production scale of the BmR1 recombinant antigen to meet the increasing demand for Brugia Rapid[trade], a commercial diagnostic test for detection of brugian filariasis. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 10, 2009 Category: Microbiology Authors: A. Norsyahida, N. Rahmah, R.M.Y. Ahmad Source Type: journals

Effect of heat treatment on Cronobacter spp. in reconstituted, dried infant formula: preparation guidelines for manufacturersemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Reconstituted DIF in water at >70°C in larger volumes, minimizing storage time before feeding and storing unused reconstituted formulate at (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 8, 2009 Category: Microbiology Authors: P.-C. Chen, T. Zahoor, S.-W. Oh, D.-H. Kang Source Type: journals

Legionella in industrial cooling towers: monitoring and control strategiesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: For effective decontamination of outdoor industrial cooling towers, disinfectants should be distributed in a targeted way, taking into account the possible sources of contamination.Significance and Impact of the Study: The data of the research permitted to modify the procedure of disinfection for better reduce the water and aerosol contamination and consequently the exposure risk. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 6, 2009 Category: Microbiology Authors: A. Carducci, M. Verani, R. Battistini Source Type: journals

The use of chromogenic bacteria as coloured substitutes for pathogens: A simple strategy during design and development of a new method for sample pretreatmentemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: Visibility of the pigmented bacteria within the complex sample matrices served to allocate bacterial content during the various steps necessary for finalization of the method protocol. The presumptive bacterial targets can be allocated simply by visualization of their bright red colour silhouetted against the background sample matrix.Significance and Impact of Study: The use of pigmented bacteria as substitutes for actual colourless target bacteria during design and development of a bacterial isolation method is a simple and inexpensive application. It saves a huge amount of time and resources, as the proof of ...
Source: Letters in Applied Microbiology - October 6, 2009 Category: Microbiology Authors: P. Rossmanith, K. Frühwirth, B. Süß, E. Schopf, M. Wagner Source Type: journals

Unravelling Escherichia coli dynamics close to the maximum growth temperature through heterogeneous modellingemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The heterogeneous model accurately describes disturbed growth curves at superoptimal temperatures, except for high initial cell densities.Significance and Impact of the Study: This study strengthens the hypothesis of the existence of a (small) heat-resistant subpopulation in typical inoculum cultures of E. coli K12 MG1655. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - October 6, 2009 Category: Microbiology Authors: E. Van Derlinden, K. Bernaerts, J.F. Van Impe Source Type: journals

The in vitro antibiofilm activity of selected culinary herbs and medicinal plants against Listeria monocytogenesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Although most extracts were able to reduce initial cell attachment, inhibition of growth in a preformed biofilm was more difficult to achieve.Significance and Impact of the Study: The ability to reduce biofilm biomass as shown by several plant extracts warrants further investigation to explore the use of natural products in antibiofilm adhesion. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 29, 2009 Category: Microbiology Authors: M. Sandasi, C.M. Leonard, A.M. Viljoen Source Type: journals

Rapid and sensitive detection of Vibrio cholerae by loop-mediated isothermal amplification targeted to the gene of outer membrane protein ompWemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: The developed LAMP assay targeting ompW gene was rapid, specific and sensitive for V. cholerae detection.Significant and Impact of the study: The developed LAMP assay appears to be precise, accurate and a valuable tool for detection of V. cholerae. This assay can replace laborious biochemical tests for the identification of V. cholerae in contaminated food sample. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 29, 2009 Category: Microbiology Authors: C. Srisuk, P. Chaivisuthangkura, S. Rukpratanporn, S. Longyant, P. Sridulyakul, P. Sithigorngul Source Type: journals

Sodium chloride affects Listeria monocytogenes adhesion to polystyrene and stainless steel by regulating flagella expressionemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Only high salinity levels, close to nongrowth conditions, repressed the expression of flagella, and consequently, decreased the adhesion capability of L. monocytogenes.Significance and Impact of the Study: Adhesion of L. monocytogenes to inert surfaces depends on environmental conditions that affect flagellum expression. High salinity concentrations would delay biofilm formation. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 28, 2009 Category: Microbiology Authors: D. Caly, D. Takilt, V. Lebret, O. Tresse Source Type: journals

Heterologous production of the Piromyces equi cinnamoyl esterase in Trichoderma reesei for biotechnological applicationsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: The synthetic gene was successfully cloned and overexpressed in T. reesei. EstA from P. equi was demonstrated to efficiently release FA from various natural substrates.Significance and Impact of the Study: Recombinant EstA produced in an industrial enzyme producer, T. reesei, was biochemically characterized, and its capacity to release an aromatic compound (FA) for biotechnological applications was demonstrated. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 24, 2009 Category: Microbiology Authors: L. Poidevin, A. Levasseur, G. Paës, D. Navarro, S. Heiss-Blanquet, M. Asther, E. Record Source Type: journals

Optimization of medium constituents for improved chitinase production by Paenibacillus sp. D1 using statistical approachemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The important factors controlling the production of chitinase by Paenibacillus sp. D1 were identified as urea, K2HPO4, chitin and yeast extract. Statistical approach was found to be very effective in optimizing the medium components in manageable number of experimental runs with overall 2·56-fold increase in chitinase production.Significance and Impact of the Study: The present investigation provides a report on statistical optimization of medium components for improved chitinase production by Paenibacillus sp. D1. Paenibacillus species are gram-positive, spore-forming bacteria with several PGPR and biocontro...
Source: Letters in Applied Microbiology - September 23, 2009 Category: Microbiology Authors: A.K. Singh, G. Mehta, H.S. Chhatpar Source Type: journals

Effect of oral immunization with Aeromonas hydrophila ghosts on protection against experimental fish infectionemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Oral immunization with A. hydrophila ghosts can elicit systemic and mucosal adaptive immune responses and has higher potential to induce protective adaptive immunity than normal vaccine.Significance and Impact of the Study: Oral immunization with bacterial ghosts is a promising new solution with potential application to prevent diseases in fish. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 22, 2009 Category: Microbiology Authors: F.P. Tu, W.H. Chu, X.Y. Zhuang, C.P. Lu Source Type: journals

Ratios between Alpha-, Beta- and Gamma-proteobacteria in tap water determined by the ProteoQuant assayemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Large-scale tap water screening using ProteoQuant revealed a dominance of Beta-proteobacteria and a potential interaction between Alpha- and Beta-proteobacteria. Gamma-proteobacteria, on the other hand, seemed independent of the two other phyla.Significance and Impact of the Study: The ProteoQuant assay will potentially be important for future understanding of the ecological forces shaping the tap water microbiota. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 22, 2009 Category: Microbiology Authors: K. Rudi, F. Berg, E. Gaustad, T. Tannes, M. Vatn Source Type: journals

Copper and cadmium: responses in Pseudomonas putida KT2440email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: These findings support that a soil pseudomonad restricts internalization of the metals by using different sets of binding proteins and efflux pumps. Activation of mechanisms to protect against oxidative stress also was evident especially with Cd exposure.Significance and Impact of the Study: The differential cellular responses to Cd and Cu suggest that risk assessment for Cd and Cu should be different. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 21, 2009 Category: Microbiology Authors: C.D. Miller, B. Pettee, C. Zhang, M. Pabst, J.E. McLean, A.J. Anderson Source Type: journals

Effects of chlorophyllin on replication of poliovirus and bovine herpesvirus in vitroemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: These results demonstrated that CHLN could be used as an antiviral suggesting directed activity on virus particles and on virus-receptor sites to BoHV. For poliovirus, CHLN also demonstrated virucide activity, moreover, showed to inhibit early steps of the replication cycle.Significance and Impact of the Study: CHLN demonstrated promising selectivity index for both virus strains; therefore, it can be used for the development of an antiviral agent. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 21, 2009 Category: Microbiology Authors: F.J. Benati, F. Lauretti, L.C. Faccin, B. Nodari, D.V. Ferri, M.S. Mantovani, R.E.C. Linhares, C. Nozawa Source Type: journals

The emerging methicillin-resistant Staphylococcus aureus ST398 clone can easily be typed using the Cfr9I SmaI-neoschizomeremail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: SmaI-PFGE is regarded as the 'gold standard' for typing S. aureus. Because of different susceptibility of the GGGCCC recognition sites of the ST398 DNA against SmaI, XmaI and Cfr9I, the proposed protocol is a valuable tool for ST398 typing.Significance and Impact of the Study: The use of this protocol allows the comparison of results from SmaI-nontypeable isolates with S. aureus SmaI-PFGE databases and can be applied for outbreak investigations and traceability studies of this emerging MRSA clone. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 21, 2009 Category: Microbiology Authors: M.A. Argudín, M.R. Rodicio, B. Guerra Source Type: journals

Histopathological studies of sclerotia of Rhizoctonia solani parasitized by the EGFP transformant of Trichoderma virensemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Trichoderma virens was able to parasitize sclerotia to make sclerotia soft and decayed, and its colonization was mainly intercellar in sclerotial tissues.Significance and Impact of the Study: This is first report of parasitism of sclerotia of R. solani by EGFP-tagged transformant, providing useful information for using T. virens as effective biocontrol agent. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 17, 2009 Category: Microbiology Authors: L.-N. Liu, J.-Z. Zhang, T. Xu Source Type: journals

Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwateremail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: TCE biotransformation can be significantly enhanced through the addition of selected plant terpenoids.Significance and Impact of the Study: Plant terpenoid and nutrient supplementation to groundwater might provide an environmentally benign means of enhancing the rate of in situ TCE bioremediation. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 17, 2009 Category: Microbiology Authors: J.R.-M. Brown, I.P. Thompson, G.I. Paton, A.C. Singer Source Type: journals

Rapid identification of bacterial isolates from aqueous kava (Piper methysticum) extracts by polymerase chain reaction and DNA sequencingemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Kava rhizome contains large amounts of starch and fibre, which justify the presence of polysaccharide-degrading bacteria in the extracts. Bacillus cereus group and Staphylococcus species may produce toxins and cause foodborne illness.Significance and Impact of the Study: The results of this study provide fundamental information that may be used to enhance the microbial quality and safety of kava beverages. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 17, 2009 Category: Microbiology Authors: P. Kandukuru, A.S. Huang, J. Dong, H.C. Bittenbender, Y. Li Source Type: journals

In vitro antiviral activity of Melaleuca alternifolia essential oilemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: These data show that TTO has an antiviral activity against influenza A/PR/8 virus subtype H1N1 and that antiviral activity has been principally attributed to terpinen-4-ol, the main active component.Significance and Impact of the Study: TTO should be a promising drug in the treatment of influenza virus infection. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 17, 2009 Category: Microbiology Authors: A. Garozzo, R. Timpanaro, B. Bisignano, P.M. Furneri, G. Bisignano, A. Castro Source Type: journals

Detection of the tdh and trh genes in Vibrio parahaemolyticus isolates in fish and mussels from Middle Black Sea Coast of Turkeyemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: It is the first report demonstrating the presence of potential pathogenic V. parahaemolyticus isolates from the Black Sea seafoods by PCR detection of tl, trh and tdh genes that was found more rapid than bacteriological methods.Significance and Impact of the Study: This study confirmed the previous reports that characterization of potential pathogenic V. parahaemolyticus isolates based on the PCR techniques was reliable and cost-effective. These results suggest that molecular detection methods should be included in Turkish Standards of seafood control in addition to bacteriological methods. (Source: Letters in...
Source: Letters in Applied Microbiology - September 9, 2009 Category: Microbiology Authors: G. Terzi, Ö. Büyüktanır, N. Yurdusev Source Type: journals

Nested real-time PCR for hepatitis A detectionemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: We have developed a novel NRT-PCR method capable of detecting as little as 0·2 PFU of HAV, which is significantly more sensitive than any other PCR technique tested in our system.Significance and Impact of the Study: NRT-PCR provides a potentially useful method for detecting HAV at extremely low levels, as frequently found in food samples, and can be potentially adopted as a regulatory method to ensure food safety. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - September 3, 2009 Category: Microbiology Authors: Y. Hu, I. Arsov Source Type: journals

Assessment of cell surface properties and adhesion potential of selected probiotic strainsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: The results suggest that Bif. longum B6 and Lact. rhamnosus GG can be candidate probiotics available for human consumption.Significance and Impact of the Study: Because the use of probiotic strains has been more concerned with their beneficial effects in the GI tract, it is essential to examine the potential of probiotic strains based on the physicochemical properties in terms of bacterial-binding and adhesion capabilities. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 31, 2009 Category: Microbiology Authors: H. Xu, H.S. Jeong, H.Y. Lee, J. Ahn Source Type: journals

Effects of yeast probiotic formulation on viability, revival and protection against infection with Salmonella enterica ssp. enterica serovar Typhimurium in miceemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Product A contained the highest number of viable cells and, fed to mice, gave the highest counts of viable yeasts and the longest persistence in faeces. Probably as a consequence, the highest survival and protection of intestinal and hepatic tissues were observed when product A was used for mouse treatment. Product E showed low counts in the formulation and was not recovered from mouse intestine.Significance and Impact of the Study: Formulation (lyophilization or aqueous suspension) is an important factor for revival and survival of a probiotic product in vivo and consequently for its protective properties. (S...
Source: Letters in Applied Microbiology - August 28, 2009 Category: Microbiology Authors: F.S. Martins, L.C. Veloso, R.M.E. Arantes, J.R. Nicoli Source Type: journals

A single-tube nested real-time polymerase chain reaction for sensitive contained detection of Cryptosporidium parvumemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The method facilitates sensitive detection of C. parvum thanks to the nested format, while reducing the risk of laboratory contamination thanks to the single-tube, real-time fluorimetric format.Significance and Impact of the Study: The developed method may be useful for sensitive contained detection of C. parvum in environmental and food samples, after appropriate separation of oocysts. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 26, 2009 Category: Microbiology Authors: J. Minarovičová, E. Kaclíková, K. Krascsenicsová, P. Siekel, T. Kuchta Source Type: journals

Induction of production and secretion β(1→4) glucanase with Saccharomyces cerevesiae by replacing the MET10 gene with egl1 gene from Trichoderma reeseiemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The novel endoglucanase-producing brewer's yeast strains with inserted endoglucanase gene and deficient MET10 gene led to reduced content of barley [beta]-glucans, enhanced filterability and increased sulfur dioxide in fermenting wort. Thus, the cost for addition of microbial [beta]-glucanase enzyme and sulfite preparations in normal beer brewing processes could be reduced.Significance and Impact of the Study: These results suggested that genetic engineering approach is a powerful tool to construct the novel recombinant brewer's yeast strains with different properties to reduce the cost of beer brewing and imp...
Source: Letters in Applied Microbiology - August 25, 2009 Category: Microbiology Authors: Y. Lu, T.-H. Wang, X.-L. Ding Source Type: journals

Changes in membrane fatty acids of Lactobacillus helveticus during vacuum drying with sorbitolemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The physiological adaptation of cells occurred with cell membrane of Lact. helveticus during vacuum drying of cells in the presence of sorbitol.Significance and Impact of the Study: The study showed that physiological adaptation with membrane of the cells occurred during the drying process. The insight implies that instead of viability improvement of dried cells by the conventional stress induction during cultivation, the induction may be exercised thereafter without compromising growth of the cells. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 25, 2009 Category: Microbiology Authors: C. Santivarangkna, U. Kulozik, H. Kienberger, P. Foerst Source Type: journals

Comparison of in vitro models to study bacterial adhesion to the intestinal epitheliumemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: The results suggested that bacterial strains exhibit different capacities to adhere to cellular components and several types of mucin present in different models, showing preferences for intestinal MUC2.Significance and impact of the study: The use of Caco-2 cells monolayer plus mucin (type II) better approaches the physiological characteristics of in vivo situation, providing a reliable and suitable in vitro model to evaluate bacterial adhesion. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 24, 2009 Category: Microbiology Authors: J.M. Laparra, Y. Sanz Source Type: journals

Identification of coliform genera recovered from water using different technologiesemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: A wide range of coliform genera were detected using both types of methods. There was considerable overlap between the two groups, and whilst differences were seen between the genera isolated with the two method types, no clear pattern emerged. Substantial numbers of 'new' coliforms (e.g. Raoutella spp.) were recovered using both types of methods.Significance and Impact of the Study: The results presented here confirm that both methods based on lactose fermentation or detection of [beta]-d-galactosidase activity recover a range of coliform organisms. Any suggestion that only methods which are based upon ferment...
Source: Letters in Applied Microbiology - August 21, 2009 Category: Microbiology Authors: C.R. Fricker, B.J. Eldred Source Type: journals

Effect of production conditions on the stability of a human bifidobacterial species Bifidobacterium longum in yogurtemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: In this investigation, the human bifidobacterial strain Bif. longum survived adequately in yogurt, although the fermentation temperature and starter composition affect bifidobacterial survival.Significance and Impact of the Study: This investigation indicates that stable probiotic yogurt using human bifidobacteria can be produced by choosing optimal production conditions. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 21, 2009 Category: Microbiology Authors: F. Abe, S. Tomita, T. Yaeshima, K. Iwatsuki Source Type: journals

Isolation and characterization of an atrazine-degrading Rhodococcus sp. strain MB-P1 from contaminated soilemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: We have successfully isolated a Rhodococcus sp. strain MB-P1 which is capable of utilizing atrazine as sole source of carbon and energy at very high concentrations of 1000 ppm. The pathway for degradation of atrazine has also been determined. The metabolic gene(s) responsible for atrazine degradation was found to be plasmid-encoded.Significance and Impact of the Study: Rhodococcus sp. strain MB-P1 could be used as an ideal model system for in-situ degradation and restoration of ecological niches which are heavily contaminated with atrazine. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 21, 2009 Category: Microbiology Authors: Fazlurrahman, M. Batra, J. Pandey, C.R. Suri, R.K. Jain Source Type: journals

Bacterial contamination on touch surfaces in the public transport system and in public areas of a hospital in Londonemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Hand-touch sites in London are frequently contaminated with bacteria and can harbour MSSA, but none of the sites tested were contaminated with MRSA.Significance and Impact of the Study: Hand-touch sites can become contaminated with staphylococci and may be fomites for the transmission of bacteria between humans. Such sites could provide a reservoir for community-associated MRSA (CA-MRSA) in high prevalence areas but were not present in London, a geographical area with a low incidence of CA-MRSA. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 21, 2009 Category: Microbiology Authors: J.A. Otter, G.L. French Source Type: journals

Production of tylosin in solid-state fermentation by Streptomyces fradiae NRRL-2702 and its gamma-irradiated mutant (γ-1)email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusion: The study has shown that solid-state fermentation system significantly enhanced the tylosin yield by mutant [gamma]-1.Significance and Impact of the Study: This study proved to be very useful and resulted in 6·87 ± 0·30-fold increase in tylosin yield by this mutant when compared to that of wild-type strain. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 21, 2009 Category: Microbiology Authors: Shazia Khaliq, Nosheen Rashid, Kalsoom Akhtar, Muhammad Afzal Ghauri Source Type: journals

Effects of moderately high pressure plus heat on the germination and inactivation of Bacillus cereus spores lacking proteins involved in germinationemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Both inactivation and germination of B. cereus spores by MHP increased with rise of temperature; however, mutant strains lacking individual germinant receptor had similar levels of germination as compared to wild-type spores. To evaluate the role of germinant receptors in MHP, a strain lacking a large number of germinant receptors is needed.Significance and Impact of the Study: The results of this work may lead to a better understanding of how MHP causes germination of spores of B. cereus. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 21, 2009 Category: Microbiology Authors: J. Wei, P. Setlow, D.G. Hoover Source Type: journals

Identification of Oxalobacter formigenes in the faeces of healthy catsemail this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Demonstrating the presence of O. formigenes in the faeces of healthy cats for the first time in this study.Significance and Impact of the Study: Future investigation of the role of this organism in the pathophysiology of calcium oxalate urolithiasis in cats is indicated. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 21, 2009 Category: Microbiology Authors: J.S. Weese, H.E. Weese, J. Rousseau Source Type: journals

Epitope mapping of pathogenic Leptospira LipL32email this articleEmail this article to a colleague. save this article to My ClippingsSave this article to My Clippings. discuss this articleDiscuss or comment on this article.
Conclusions: Epitope identification and analysis suggested the existence of two antigenic regions within LipL32. These LipL32 reactive regions were highly conserved among antigenically variants of Leptospira spp. isolates. Peptides containing these regions (P1 and P2) showed a good capability for anti-leptospiral antibody recognition.Significance and Impact of the Study: This finding could have potential relevance not only for serodiagnosis but also as a starting point for the characterization of targets for vaccine design. (Source: Letters in Applied Microbiology)
Source: Letters in Applied Microbiology - August 21, 2009 Category: Microbiology Authors: Javier Lottersberger, Sergio A. Guerrero, Georgina G. Tonarelli, Ronald Frank, Héctor Tarabla, Norma B. Vanasco Source Type: journals