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Medical molecular morphology with imaging mass spectrometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Imaging mass spectrometry (IMS) is a two-dimensional mass spectrometry to visualize the spatial distribution of biomolecules that does not need either separation or purification of target molecules and enables us to monitor not only the identification of unknown molecules but also the localization of numerous molecules simultaneously. Among the ionization techniques, matrix-assisted laser desorption/ionization (MALDI) is one of those most generally used for IMS, which allows the analysis of numerous biomolecules ranging over wide molecular weights. At present, targets of IMS research have expanded t...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Primitive neuroectodermal tumor of the kidneyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a 21-year-old man with a primitive neuroectodermal tumor (PNET) of the kidney that was difficult to distinguish from other small round cell tumors, e.g., small cell carcinoma, malignant lymphoma, and Wilms’ tumor. Pathologically, a primitive rosette-like pattern was shown by H&E staining; expression of MIC-2 was indicated by an immunohistochemical test; rather primitive organelles were observed by an ultrastructural method; and translocation of chromosome 22 was confirmed by FISH. We therefore diagnosed the current case as PNET. The patient had undergone a right radical nephrectomy more than 1.5 years earli...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Phagocytosis mechanism of apoptotic granulosa cells regulated by milk-fat globule-EGF factor 8Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract In the process of ovary sexual maturation, most immature ovarian follicles degrade into atretic follicles accompanied by apoptosis in granulosa cells. Macrophages can recognize apoptotic cells through specific binding with phosphatidylserine (PS), exposed on the surface of apoptotic cells, which is mediated by milk-fat globule-EGF factor 8 (MFG-E8). In the present research, we examined the involvement of the MFG-E8-dependent phagocytosis system in the atretic follicles of developing mouse ovaries. The number of atretic follicles and DNA-fragmented granulosa cells significantly increased in B6C3F1 mi...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Pancreatic-type mixed acinar-endocrine carcinoma with alpha-fetoprotein production arising from the stomach: a report of an extremely rare caseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract An extremely rare case of mixed acinar-endocrine carcinoma (MAEC) arising from the stomach in a 56-year-old Japanese woman is herein presented. An endoscopic examination and computed tomography showed a protruding gastric tumor and a large extragastric mass, respectively. Macroscopic observation on the surgical specimen revealed the extragastric cystic mass was continued to the intragastric tumor. Histologically, the intragastric tumor consisted of large or small solid nests with acinar appearance. The cancer cells had an ovoid nuclei and polygonal cytoplasm, which was frequently amphophilic. Immuno...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Impact of hepatitis B virus X protein on the DNA damage response during hepatocarcinogenesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal cancers worldwide. The main HCC-associated diseases are chronic infections with hepatitis B virus (HBV) and hepatitis C virus (HCV), and HBV-associated HCC is still prevalent in Asia. Many studies have suggested that HBV X protein (HBX), which is the most common ORF integrated into the host genome, plays a crucial role in hepatocarcinogenesis. However, the accumulated evidence regarding HBX-mediated signaling pathways is not concordant, and it is difficult to understand the mechanistic nature of HBX-associated hepatocarcinogenesi...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

The immunohistochemical expression profile of osteopontin in normal human tissues using two site-specific antibodies reveals a wide distribution of positive cells and extensive expression in the central and peripheral nervous systemsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, we demonstrate that OPN is widely distributed in normal human cells, particularly those comprising the central and peripheral nervous systems. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-009-0459-6Authors Yasuto Kunii, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 JapanShin-ichi Niwa, Fukushima Medical University School of Medicine Department of Neuropsychiatry Fukushima JapanYoshiaki Hagiwara, Immunobiological Laboratories Takasaki JapanMasahiro Maeda, Immunobiological Laboratories Takasaki JapanTsutomu Seitoh, Immun...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Extraskeletal myxoid chondrosarcoma: a study using a quick-freezing and deep-etching methodEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract A case of extraskeletal myxoid chondrosarcoma (ESMC), which developed in the right thigh of a middle-aged Japanese woman, was studied using immunohistochemistry, conventional electron microscopy, and the quick-freezing and deep-etching (QF-DE) method. In addition to typical light microscopic findings of ESMC, conventional electron microscopy indicated that the tumor cells had features of chondrocytes. Immunohistochemically, the tumor cells showed a positive immunoreaction for S100 protein. A diagnosis of ESMC was made. An interesting observation was the ultrastructural features of collagen fibrils i...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Immunohistochemical and electron microscopy studies of a case of hyalinizing trabecular tumor of the thyroid gland, with special consideration of the hyalinizing mass associated with itEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report here a case of HTT with cytological, immunohistochemical, and ultrastructural findings, focused in particular on the hyalinizing mass. Cytologically, tumor cells exhibiting many intranuclear cytoplasmic inclusions and nuclear grooves were found in association with light green-positive, irregular, fluffy membranous structures on touch smear. Staining with antibody to collagen type IV was positive in these membranous structures. Histopathologically, tumor cells exhibited many intranuclear cytoplasmic inclusions, and were positive for staining with antibodies to S100 protein, neuron-specific enolase, thyroglobu...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Clear cell chondrosarcoma: an ultrastructural studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report here a case of CCC. A 67-year-old Japanese man presented with right arthralgia for 1 year, and histological examination of the subsequent surgical resection of the right femoral bone showed the finding of CCC. Ultrastructurally, most organelles were observed in the perinuclear area. Clear neoplastic cells contained many glycogen particles in the area of the cytoplasm lacking organelles, although glycogen particles overall seemed to be evenly distributed in the cytoplasm. Some mitochondria, Golgi complex, actin-like filaments, and rough endoplasmic reticulum were also demonstrated in the cytoplasm of clear ce...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Morphometric analysis of regional lymph nodes in surgically resected non-small cell lung cancerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Nodal staging is a crucial factor in choosing the treatment option for non-small cell lung cancer (NSCLC). However, so far as we know, a computer-based histomorphometric analysis of lymph nodes in NSCLC has never been developed. We studied 299 surgically resected lymph nodes from 108 patients with NSCLC. Microscopic digital images were analyzed with Scion Image software. Seventy lymph nodes had at least one metastatic focus. The metastasis occupancy area per node ranged from 0.01 to 209.58 mm2 (mean, 29.58 ± 5.87 mm2). The metastasis occupancy ratio ranged from 0.01% to 100% (mean, 48.70% ± 42.10%)...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Environmental factors involved in axonal regeneration following spinal cord transection in ratsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract A recent study of a rat model treated with grafted collagen filament (CF) after spinal cord transection showed dramatic recovery of motor function but did not report on the acute-stage phenomenon. In the present study, we describe molecular and histological aspects of the axonal regeneration process during the acute stage following spinal cord transection. The spinal cord of 8-week-old rats was completely transected, and a scaffold of almost the same size as the resected portion was implanted in the gap. Changes in the mRNA expression of four neurotrophic factors [nerve growth factor (NGF), brain-de...
Source: Medical Molecular Morphology - September 29, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Expression of endothelin system in neuroblastic tumors: close association of endothelin-1 and endothelin B receptor expression with differentiation of tumor cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, ET-1 and ET-B are expressed with differentiation of neuroblastic tumors. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0437-4Authors Nobuo Hoshi, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 JapanTakashi Sugino, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 JapanToshimitsu Suzuki, Fukushima Medical University School of Medicine Department of Pathology 1 Hikariga-oka Fukushima 960-1295 Japan Journal Medical Molecular MorphologyOnline ISSN 1860-1499Pri...
Source: Medical Molecular Morphology - June 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Unique mode of cell death in freshly isolated adult rat ventricular cardiomyocytes exposed to hydrogen peroxideEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract To address whether adult rat ventricular cardiomyocytes (ARVCs) exposed to oxidant stress die via apoptosis (secondarily by necrosis) or primarily by necrosis, we exposed ARVCs to hydrogen peroxide (H2O2; 0.1–100 μM) for up to 24 h and then compared them with isoproterenol-induced apoptotic and Triton X-induced necrotic controls. Cellular shrinkage preceded plasma membrane disruption, reflected by trypan blue uptake in ARVCs exposed to lower concentrations of H2O2 (<1 μM; an apoptotic pattern), but the order was reversed in cells exposed to higher concentrations of H2O2 (>1 μM; a necrotic ...
Source: Medical Molecular Morphology - June 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Nucleocytoplasmic transport of luciferase gene mRNA requires CRM1/Exportin1 and RanGTPaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Human immunodeficiency virus type 1 Rev (regulator of the expression of the virion) protein was shown to reduce the expression level of the co-transfected luciferase reporter gene (luc+) introduced to monitor transfection efficiency. We studied the mechanism of the inhibitory Rev effect. The effect, caused by nuclear retention of luc+ mRNA, was reversed if rev had a point mutation that makes its nuclear export signal (NES) unable to associate with cellular transport factors. The Rev NES receptor CRM1 (chromosome region maintenance 1)-specific inhibitor, leptomycin B, blocked luc+ mRNA export. This fi...
Source: Medical Molecular Morphology - June 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Changes in the expression of E-cadherin repressors, Snail, Slug, SIP1, and Twist, in the development and progression of ovarian carcinoma: the important role of Snail in ovarian tumorigenesis and progressionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Changes in the expression of E-cadherin have been reported to be important in the tumorigenesis and progression of epithelial ovarian carcinoma. To further examine the mechanisms regulating E-cadherin expression in ovarian tumorigenesis, we investigated the immunohistochemical expression of transcriptional repressors for E-cadherin, such as Snail, Slug, SIP1, and Twist, in the ovarian surface epithelium (OSE) and 95 cases of epithelial ovarian tumors. OSE cells were negative for SIP1 and Slug, whereas weak expression of Snail and Twist was observed in 8 (73%) and 3 (27%) cases, respectively. Of 95 o...
Source: Medical Molecular Morphology - June 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian liversEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Arylsulfatase A (ARSA) and B (ARSB) have been regarded as lysosomal enzymes because of their hydrolytic activity on synthetic aromatic substrates and the lysosomal localization of their enzymatic activity. Using sea urchin embryos, we previously demonstrated that the bulk of ARS is located on the cell surface of the epithelium, colocalizing with sulfated polysaccharides, and that it does not exhibit enzymatic activity. To examine whether ARSA and ARSB exist on the cell surface in mammalian tissues, we raised antibodies against ARSA and ARSB and examined immunohistochemically their localization in th...
Source: Medical Molecular Morphology - June 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Hepatoid carcinoma of the skin: spontaneous rat skin hepatoid carcinoma with eosinophilic globules and crystals immunoreactive to α-1-antitrypsinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present a case of hepatoid carcinoma of the abdominal skin in a male Wistar rat. Histopathologically, this carcinoma resembled human hepatocellular carcinoma with respect to trabecular-sinusoidal structures. Carcinoma tissues contain numerous eosinophilic globules and crystals, and in this case, we found the characteristic eosinophilic globules in the hepatoid carcinoma cells and the crystals in the extracellular portions. Vivid carcinoma cells full of eosinophilic globules were present near the necrotic areas in tumor tissue, wherein quadrate crystals unstained with eosin were observed. PAS staining after diastase ...
Source: Medical Molecular Morphology - June 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

KL-6 is another useful marker in assessing a micropapillary pattern in carcinomas of the breast and urinary bladder, but not the colonEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract To evaluate the peculiar “inside-out” pattern in micropapillary (MP) carcinoma, we investigated the usefulness of KL-6 antibody in the assessment of the MP pattern of cancers, in comparison with antibodies to epithelial membrane antigen (EMA), MUC1 (CD227), and CD 10. Immunohistochemical investigation was performed on specimens exhibiting an MP pattern obtained from 12 persons with cancer: 4 with breast carcinoma, 3 with carcinoma of the urinary bladder, and 5 with colonic carcinoma. Immunohistochemical study with KL-6, EMA, and MUC1 antibodies revealed similar continuous linear positive pattern...
Source: Medical Molecular Morphology - June 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Expression profiling of micro-RNAs in human esophageal squamous cell carcinoma using RT-PCREmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we analyzed the expression of mature miRNAs in esophageal squamous cell carcinoma (ESCC). The expression of 73 miRNAs was quantified by qRT-PCR in 30 primary ESCC specimens. We examined the correlation between miRNA expressions and the clinicopathological factors and prognosis of ESCC. The Kaplan-Meier survival curves showed that the high expression levels of 6 of the 72 miRNAs correlated with significantly lower patient survival rates. The overexpression of miR-129 was identified as a significant and independent prognostic factor (P = 0.031) in surgically treated ESCC patients. The hazard ratio for the...
Source: Medical Molecular Morphology - June 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

The majority of triple-negative breast cancer may correspond to basal-like carcinoma, but triple-negative breast cancer is not identical to basal-like carcinomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Recently, the concept of basal-like carcinoma has been proposed. However, there are only a few reports about the relationship between triple-negative cancer and basal-like carcinoma. In this article, we report the study of the expression of basal cell markers in 11 triple-negative cancers. Eight tumors (4 metaplastic carcinomas, 2 invasive ductal carcinomas, 1 invasive papillary carcinoma, and 1 medullary carcinoma) were positive for more than three markers among cytokeratins 5, 14, and 17, and p63. Three tumors (2 invasive ductal carcinomas and 1 apocrine carcinoma) were completely negative for all...
Source: Medical Molecular Morphology - June 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Nanotransportation system for cholera toxin in Vibrio cholerae 01Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we determined that the pH-dependent intrabacterial transport system correlates with the priming of CT secretion by V. cholerae. The secretion and production of V. cholerae treated at different pHs were examined by enzyme immunoassay. The localization of the CT was analyzed by immunoelectron microscopy. The CT secretion level rapidly increases in the alkaline-pH-treated V. cholerae but does so more slowly in neutral- and acidic-pH-treated V. cholerae. The CT was found to be densely localized near the membrane in the alkaline-pH-treated bacterial cytoplasm, suggesting that the CT shifts from the center to t...
Source: Medical Molecular Morphology - March 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Ultrastructural immunolocalization of a cartilage-specific proteoglycan, aggrecan, in salivary pleomorphic adenomasEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract A pleomorphic adenoma (PA) is the most common epithelial tumor in the salivary glands, but it frequently shows a mesenchyme-like histology, including the presence of myxoid and chondroid areas. Cartilage-specific matrix proteins are deposited in PA. Aggrecan is a major component of cartilage-specific proteoglycans. The present study examined the ultrastructure of the stromal areas in ten salivary PA specimens and investigated the distribution of aggrecan by immunoelectron microscopy. Aggrecan was deposited in the myxoid and chondroid stroma of PA. Ultrastructural observations revealed many proteogly...
Source: Medical Molecular Morphology - March 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Immunolocalization of phospho-Arg-directed protein kinase-substrate in hypoxic kidneys using in vivo cryotechniqueEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, normal and hypoxic mouse kidneys were prepared using an “in vivo cryotechnique” (IVCT) and examined immunohistochemically with specific antibodies against phospho-(Ser/Thr) PKA/C substrate (P-PK-S) and phospho-(Ser/Thr) Akt substrate (P-Akt-S) to capture their time-dependent regulation in vivo. Left kidneys were cryofixed with IVCT under normal blood circulation and after varying hypoxic intervals, followed by freeze-substitution with acetone containing paraformaldehyde. Deparaffinized sections were immunostained for P-PK-S, Na+/HCO3 − cotransporter NBC1, and a membrane skeletal protein, 4.1B. Th...
Source: Medical Molecular Morphology - March 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Apoptotic effects of Tian-Long compound on endometrial adenocarcinoma cells in vitroEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The Tian-Long (TL) compound is a water-soluble extract of six Chinese medicinal herbs. To explore its antitumor properties and the mechanism for activity in gynecological malignancies, the present studies were carried out using Ishikawa cells derived from uterine endometrial adenocarcinoma. Morphologically, cell death and decrease in the number of viable cells were observed in the presence of the TL compound. The proliferation of Ishikawa cells was significantly suppressed in a time- and dose-dependent manner, as indicated by both the WST-1 and the BrdU incorporation assay. Results from both the WST...
Source: Medical Molecular Morphology - March 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Layer-specific sulfatide localization in rat hippocampus middle molecular layer is revealed by nanoparticle-assisted laser desorption/ionization imaging mass spectrometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we solved the problem by our recently developed nanoparticle-assisted laser desorption/ionization (nano-PALDI)-based imaging mass spectrometry (IMS). We revealed that the level of sulfatide in the middle molecular layer was significantly higher than that in granule cell layers and the inner molecular layer in the dentate gyrus of rat hippocampus. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0427-6Authors Hiroshi Ageta, Mitsubishi Kagaku Institute of Life Sciences (MITILS) Tokyo JapanSayaka Asai, Mitsubishi Kagaku Institute of Life Sciences (MITILS) Tokyo JapanYuki Sugiura...
Source: Medical Molecular Morphology - March 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Interaction of a multi-domain adaptor protein, vinexin, with a Rho-effector, RhotekinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Among various effector proteins for the Rho small GTPase, the function(s) of Rhotekin is almost unknown. We have identified a multi-domain adaptor protein, vinexin, as a binding partner for Rhotekin, using yeast two-hybrid screening of a human heart library. Rhotekin was found to associate with vinexin in vitro, in COS7 cells, and in brain tissues. The C-terminal Pro-rich motif of Rhotekin exhibited binding to the third SH3 domain of vinexin. The binding was little affected by Rho but was inhibited by activated Cdc42 in COS7 cells. Immunofluorescence analyses revealed partial colocalization of vinex...
Source: Medical Molecular Morphology - March 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Natural killer cell receptors and their ligands in liver diseasesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The liver is a distinctive immune organ with predominant innate immunity, being rich in innate immune cells such as natural killer (NK) cells. In humans, NK cells comprise about 30%–50% of intrahepatic lymphocytes, whereas peripheral blood lymphocytes contain about 5%–20% NK cells. Accumulating evidence suggests that NK cells play an important role not only in host defense against invading microorganisms and tumor transformation in the liver but also in liver injury and repair. In recent years, significant progress has been made in terms of understanding how NK cells recognize their target cells...
Source: Medical Molecular Morphology - March 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Ureteral small cell carcinomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, we suggest that endocrine cells in the ureteral urothelial epithelium may be precursor cells of ureteral small cell carcinoma. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-007-0384-5Authors Naoto Kuroda, Kochi Red Cross Hospital Department of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-8562 JapanKazunobu Katto, Kochi Red Cross Hospital Department of Urology Kochi JapanMasato Tamura, Kochi Red Cross Hospital Department of Urology Kochi JapanTomoyuki Shiotsu, Kochi Red Cross Hospital Department of Urology Kochi JapanKaori Inoue, Kochi Red Cross Hospital Department of Dia...
Source: Medical Molecular Morphology - March 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Neuroendocrine small cell carcinoma of the breast: report of a caseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract A rare case of neuroendocrine small cell carcinoma (SmCC) of the breast is reported. A 51-year-old postmenopausal woman noticed a nodule approximately 3 cm in diameter in her right upper breast. Histologically, the tumor consisted of small ovoid to pleomorphic cells with hyperchromatic nuclei, and a large central area was occupied by acellular amorphous tissue. Extensive lymphatic permeation was seen around the tumor. Invasive and in situ ductal carcinoma foci were not observed in and surrounding the tumor. Immunohistochemically, estrogen and progesterone receptors and HER2/neu were all negative in ...
Source: Medical Molecular Morphology - March 18, 2009 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Coexistent adenomyoepithelioma and invasive ductal carcinoma of the breast: presentation as separate tumorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report an unusual case of adenomyoepithelioma associated with invasive ductal carcinoma here. Histologically, the lesion consisted of two separate tumors. One nodule corresponded to invasive ductal carcinoma consisting of tubular and trabecular arrangements of columnar or cuboidal neoplastic cells. The other tumor corresponded to adenomyoepithelioma consisting of an inner layer of neoplastic cells with basophilic cytoplasm and the outer layer of neoplastic cells with clear cytoplasm. Immunohistochemically, some myofibroblasts were observed in the stroma of both adenomyoepithelioma and invasive ductal carcinoma, but...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Immunohistochemical and electron microscopic observations of stromal cells in the human oviduct mucosaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Stromal cells in the lamina propria of the human oviduct mucosa are unique cells that can differentiate into decidual cells during ectopic pregnancy in the oviduct. The nature of stromal cells is still unknown. In the present study, we investigated human oviductal stromal cells with transmission electron microscopy and immunohistochemistry and revealed that they had ultrastructural features similar to myofibroblasts and expressed alpha-smooth muscle actin, a marker used to identify myofibroblasts. Primary cilia were also one of the characteristic profiles of the stromal cells. These findings showed ...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Chromophobe renal cell carcinoma: useful diagnostic application of imprint cytology and fluorescence in situ hybridization of chromosomes 10 and 21 in two cases of typical and eosinophilic variantsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, we suggest that the combination study of imprint cytology and FISH of chromosomes 10 and 21 as well as routine histology may contribute to the accurate diagnosis of chromophobe RCC. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0406-yAuthors Naoto Kuroda, Kochi Red Cross Hospital Departments of Diagnostic Pathology 2-13-51 Shin-honmachi Kochi 780-8562 JapanKazunobu Katto, Kochi Red Cross Hospital Department of Urology Kochi JapanTadanori Yamaguchi, Ayabe City Hospital Department of Pathology Kyoto JapanTakako Kawada, Maizuru Kyosai Hospital Department of Pathology Kyoto Jap...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

An autopsy case of chronic progressive external ophthalmoplegia with renal insufficiencyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract An autopsy of a 44-year-old Japanese woman with mitochondrial cytopathy confirmed the presence of chronic progressive external ophthalmoplegia (CPEO). Immunohistochemistry using antimitochondrial antibody was performed to observe the ultrastructure of the skeletal muscle and renal tissues. The patient was born of consanguineous parents, developed normally, and was of average intelligence. At 22 years of age, the patient noticed hearing loss, and subsequently, over time, developed a progressive generalized muscle weakness, which included limitation of eye movement and ptosis. At age 41, a muscle biop...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

The Kras2 oncogene and mouse lung carcinogenesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Activating point mutations of the mouse Kras2 oncogene or its human homologue, KRAS, are critical for lung adenocarcinoma genesis, independent of the species. Significantly, in the mouse, several polymorphic Kras2 alleles have been identified, which cosegregate with genetic susceptibility to chemical induction of lung tumors. Moreover, a major lung tumor susceptibility locus, the Pas1 (Pulmonary adenoma susceptibility 1), was found to colocalize with Kras2 on distal chromosome 6 on linkage analysis. The Kras2 may thus be involved in both cellular transformation and genetic control of tumor susceptibil...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Sodium azide induces necrotic cell death in rat squamous cell carcinoma SCC131Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, NaN3 induces necrotic cell death in SCC131. This experimental model may be used in the study of necrotic cell death. Content Type Journal ArticleCategory Original PaperDOI 10.1007/s00795-008-0414-yAuthors Eiju Sato, Fukushima Medical University Department of Dentistry & Oral Surgery Fukushima JapanToshimitsu Suzuki, Fukushima Medical University Department of Pathology Fukushima JapanNobuo Hoshi, Fukushima Medical University Department of Pathology Fukushima JapanTakashi Sugino, Fukushima Medical University Department of Pathology Fukushima JapanHiroshi Hasegawa, Fukushima Medical University Department o...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Ultrastructural study of the morphogenesis of human herpesvirus 6 type B in human T-lymphotropic virus type I-producing lymphoid cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract A few studies of the morphogenesis of human herpesvirus (HHV) 6 type A and B (HHV-6A, -6B) have been performed using neurogenic, lymphoid, or epithelial cells. When human MT-4 T-lymphotropic virus type I (HTLV-I)-producing lymphoid cells were coinfected with HHV-6B in vitro, viral-specific proteins were clearly detected. We therefore attempted to detect virus particles at the ultrastructural level, focusing on the morphogenesis of such particles. Ultrastructurally, HHV-6B virus particles could be observed in the nuclei, cytoplasm, and extracellular spaces of MT-4 cells, in addition to extracellular ...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

The primary cilia of secretory cells in the human oviduct mucosaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The human oviduct is lined with a simple columnar epithelium composed of ciliated cells and secretory cells. Primary cilia or solitary cilia usually extend from the apical surface of the secretory cells. The axoneme of the primary cilia is composed of nine peripheral microtubule doublets (9 + 0 pattern) that lack dynein arms and nexin links. Displacement of peripheral doublets to the central region, which is suggested to be attributable to the lack of nexin links, is one of the distinctive features of oviductal primary cilia. The basal body that extends the primary cilium connects to its paired cent...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Tooth-forming potential in embryonic and postnatal tooth bud cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This article describes the achievements to date in production of biological teeth, mostly from our laboratory. In particular, we describe the potential to produce teeth from embryonic and postnatal tooth buds. Content Type Journal ArticleCategory ReviewDOI 10.1007/s00795-008-0416-9Authors Masaki J. Honda, Nihon University School of Dentistry Department of Anatomy 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310 JapanHanson Fong, University of Washington Department of Materials Science & Engineering Seattle WA USAShinji Iwatsuki, University of Tokyo Tooth Regeneration, Division of Stem Cell Engineering, Institute of...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

A case of primary hepatic non-Hodgkin’s lymphoma with chronic hepatitis CEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Content Type Journal ArticleCategory ErratumDOI 10.1007/s00795-008-0426-7Authors Fumihiko Kaneko, Kitasato Institute Medical Center Hospital Department of Internal Medicine 6-100 Arai, Kitamoto Saitama 364-8501 JapanHiroaki Yokomori, Kitasato Institute Medical Center Hospital Department of Internal Medicine 6-100 Arai, Kitamoto Saitama 364-8501 JapanAya Sato, Kitasato Institute Medical Center Hospital Department of Internal Medicine 6-100 Arai, Kitamoto Saitama 364-8501 JapanHajime Takeuchi, Kitasato Institute Medical Center Hospital Department of Internal Medicine 6-100 Arai, Kitamoto Saitama 364-8501 JapanKumiko Taha...
Source: Medical Molecular Morphology - December 24, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Reduction of retinaldehyde dehydrogenase 1 expression and production in estrogen-induced prolactinoma of ratEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we examined the expression of RALDH1 in diethylstilbestrol-induced prolactinoma of LEXF RI rats. Quantitative analysis of mRNA levels by real-time PCR demonstrated drastic reduction of RALDH1 expression in the prolactinoma. We have also detected both mRNA expression and production by in situ hybridization and immunohistochemistry. Both mRNA-expressing cells and immunopositive cells remarkably decreased after 4 weeks of treatment with diethylstilbestrol. Fluorescence double immunohistochemistry of RALDH1 and prolactin revealed that prolactin-immunopositive cells do not colocalize with RALDH1 in the prola...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Immunohistochemical and electron microscopic characterization of brush cells of the rat cecumEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Brush cells (BCs) are relatively rare cells that are sparsely distributed throughout the mammalian digestive and respiratory systems. BCs have been identified in the rodent large intestine, but these cells have not been characterized by immunocytochemistry or electron microscopy. We previously demonstrated that rat bile duct BCs had strong immunoreactivity for six proteins that function in HCO3− secretion and thus assumed that BCs secrete NaHCO3. It is well known that the gastrointestinal (GI) tract secretes NaHCO3, but it is not known whether BCs of the GI tract also express proteins related to HC...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Raloxifene, a selective estrogen receptor modulator, induces mitochondria-mediated apoptosis in human endometrial carcinoma cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Raloxifene is a nonsteroidal benzothiophene that has also been classified as a selective estrogen receptor modulator (SERM) on the basis of studies in which it produced both estrogen-agonistic effects on bone and lipid metabolism and estrogen-antagonistic effects on uterine endometrium and breast tissue. We investigated apoptotic cell death and the apoptotic pathway in human endometrial carcinoma cells (Ishikawa cells) expressing estrogen receptor treated with raloxifene. Cell viability was significantly decreased in Ishikawa cells treated with raloxifene at 20 μM and higher levels. Raloxifene at 2...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Characteristic cell wall ultrastructure of a macrolide-resistant Staphylococcus capitis strain isolated from a patient with chronic sinusitisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Fourteen-membered-ring macrolides have an antiinflammatory effect, in addition to their antibacterial effect, and are widely used at low dosages for long-term therapy for chronic inflammatory disease such as diffuse pan-bronchiolitis and chronic sinusitis. A macrolide-resistant coagulase-negative staphylococcal strain was obtained from the maxillary sinus of a patient with chronic sinusitis, who failed long-term macrolide therapy. The isolated strain was characterized as Staphylococcus capitis and had an MIC for erythromycin greater than 128 μg/ml. Morphological observation demonstrated that this ma...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Immunoreactivities for glutathione S -transferases and glutathione peroxidase in the lateral wall of pigmented and albino guinea pig cochleaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract Dark-skinned people are known to be more tolerant of ototraumatic noise than are light-skinned people, and pigmented animals are more tolerant of ototraumatic noise and aminoglycoside ototoxicity than are albino animals. Such tolerance may be dependent on the local ability of detoxification and antioxidant enzymes, including glutathione S-transferase (GST) and glutathione peroxidase (GSPx). In the present study, we examined the difference in GST/GSPx expression in the lateral wall of the cochlea between pigmented and albino guinea pigs. Eight-week-old male pigmented and albino guinea pigs were kille...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Cytological characteristics of invasive lobular carcinoma of the human breastEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract In contrast to invasive ductal carcinoma, invasive lobular carcinoma (ILC) of the breast is characterized by multiple ipsilateral occurrences and a higher incidence in the contralateral breast. It is therefore necessary to examine thoroughly whether there is any other carcinoma present before any breast-conserving surgery is carried out. We cytologically, histologically, and ultrastructurally investigated ILC and pure scirrhous carcinoma (PSC), a subtype of invasive ductal carcinoma, to establish cytological diagnostic criteria for the differential diagnosis of these two types of carcinoma that have...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

A rare case of coexistence of pulmonary adenocarcinoma with Langerhans’ cell histiocytosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a rare case of coexisting pulmonary adenocarcinoma and Langerhans’ cell histiocytosis (LCH) in a 78-year-old woman who did not smoke. During follow-up of diabetes mellitus, she had complained of chest pain and was found to have a nodular lesion in S9 of the left lower lobe, which was resected surgically. No abnormal laboratory findings were obtained. Before surgical resection, needle biopsy specimens confirmed the existence of adenocarcinoma. The resected tumor in the left lower lobe was 3.0 × 1.8 × 3.0 cm, and histologically both acinar and bronchioloalveolar cell subtypes of adenocarcinoma were found i...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

A case of primary hepatic non-Hodgkin’s lymphoma with chronic hepatitis CEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a case of primary hepatic non-Hodgkin’s lymphoma in a 67-year-old man with chronic hepatitis C. Laboratory data revealed slightly elevated liver function parameters and positive for hepatitis C virus (HCV) antibody. Abdominal ultrasonography showed hypoechoic lesions approximately 5 mm in diameter in the whole liver. Magnetic resonance imaging showed that the tumors were isointense in relationship to the liver on T1-weighted images but were slightly hyperintense on T2-weighted images. Under a clinical diagnosis of liver tumor, liver biopsy was performed. Histological examination confirmed a diagnosis of non...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Signet-ring stromal tumor of the ovary: an immunohistochemical and ultrastructural study with a review of the literatureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report here an additional case of this type of tumor that arose from the left ovary in a 76-year-old woman. By light microscopy, the tumor was composed of small round and oval cells with cytoplasmic vacuolization and a typical signet-ring appearance, focally admixed with fibromatous tissue. Special staining revealed that the vacuoles of the tumor cells contained no lipid, mucoprotein, or glycogen. Interestingly, variously sized hyaline globule-like structures positive for periodic acid-Schiff (PAS) reaction with and without diastase digestion were present in the cytoplasm of tumor cells. Immunohistochemically, the ...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals

Distinct morphological and immunohistochemical features and different growth rates among four human neuroblastomas heterotransplanted into nude miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract To elucidate more precisely the biological characteristics of neuroblastomas, we examined four human neuroblastomas heterotransplanted into athymic nude mice NB-39 (undifferentiated type), NB-45 (poorly differentiated type with undifferentiated component), NB-52 (poorly differentiated type), and NB-726 (differentiating type) by electron microscopy, immunohistochemistry, and radioimmunoassay for the peptides in tumors. Ultrastructurally, NB-45, NB-52, and NB-726 contained more numerous and variously sized neurosecretory granules than did NB-39. Immunohistochemistry revealed neurofilament proteins, ty...
Source: Medical Molecular Morphology - September 20, 2008 Category: Molecular Biology Tags: Medical Molecular Morphology Source Type: journals