Current data processing methods and reporting standards for untargeted analysis of volatile organic compounds using direct mass spectrometry: a systematic review
ConclusionsStandardised workflows and reporting systems need to be developed to ensure research in this area is replicable, comparable, and held to a high standard. Thus, allowing the wide-ranging potential applications to be realised. (Source: Metabolomics)
Source: Metabolomics - March 16, 2024 Category: Biology Source Type: research

Plasma and serum metabolic analysis of healthy adults shows characteristic profiles by subjects ’ sex and age
ConclusionThese findings may contribute to the development of comprehensive metabolomics analyses with confounding factor-based adjustment and enhance the reliability and interpretability of future large-scale investigations. (Source: Metabolomics)
Source: Metabolomics - March 16, 2024 Category: Biology Source Type: research

Current data processing methods and reporting standards for untargeted analysis of volatile organic compounds using direct mass spectrometry: a systematic review
ConclusionsStandardised workflows and reporting systems need to be developed to ensure research in this area is replicable, comparable, and held to a high standard. Thus, allowing the wide-ranging potential applications to be realised. (Source: Metabolomics)
Source: Metabolomics - March 16, 2024 Category: Biology Source Type: research

Plasma and serum metabolic analysis of healthy adults shows characteristic profiles by subjects ’ sex and age
ConclusionThese findings may contribute to the development of comprehensive metabolomics analyses with confounding factor-based adjustment and enhance the reliability and interpretability of future large-scale investigations. (Source: Metabolomics)
Source: Metabolomics - March 16, 2024 Category: Biology Source Type: research

Perspective: use and reuse of NMR-based metabolomics data: what works and what remains challenging
AbstractBackgroundThe National Cancer Institute issued a Request for Information (RFI; NOT-CA-23-007) in October 2022, soliciting input on using and reusing metabolomics data. This RFI aimed to gather input on best practices for metabolomics data storage, management, and use/reuse.Aim of reviewThe nuclear magnetic resonance (NMR) Interest Group within the Metabolomics Association of North America (MANA) prepared a set of recommendations regarding the deposition, archiving, use, and reuse of NMR-based and, to a lesser extent, mass spectrometry (MS)-based metabolomics datasets. These recommendations were built on the collect...
Source: Metabolomics - March 13, 2024 Category: Biology Source Type: research

Comparisons of different extraction methods and solvents for saliva samples
ConclusionPPT with acetonitrile or acetone was recommended for untargeted analysis, while LLE with the mixture of chloroform and methanol was recommended for small hydrophobic compounds. (Source: Metabolomics)
Source: Metabolomics - March 9, 2024 Category: Biology Source Type: research

Untargeted NMR-based metabolomics analysis of kidney allograft perfusates identifies a signature of delayed graft function
ConclusionsThe metabolome of kidney grafts is influenced by the donor ’s type in both human and pre-clinical studies and could be correlated with DGF in the human DBD cohort. Thus, metabolomic analysis of perfusate applied prior to KTx may represent a new predictive tool for clinicians in a more personalized management of DGF. Moreover, our data paves the way to bet ter understand the impact of donor’s types on the biochemical events occurring between death and the hypothermic storage. (Source: Metabolomics)
Source: Metabolomics - March 9, 2024 Category: Biology Source Type: research

Gut metabolic changes during pregnancy reveal the importance of gastrointestinal region in sample collection
ConclusionsThe regional variation observed in the gut metabolome are likely due to the microbial and physiological differences between the different parts of the intestines. Additionally, pregnancy impacts the fecal metabolome, which may be due to evolving needs of both the dam and fetus. (Source: Metabolomics)
Source: Metabolomics - March 9, 2024 Category: Biology Source Type: research

Comparisons of different extraction methods and solvents for saliva samples
ConclusionPPT with acetonitrile or acetone was recommended for untargeted analysis, while LLE with the mixture of chloroform and methanol was recommended for small hydrophobic compounds. (Source: Metabolomics)
Source: Metabolomics - March 9, 2024 Category: Biology Source Type: research

Untargeted NMR-based metabolomics analysis of kidney allograft perfusates identifies a signature of delayed graft function
ConclusionsThe metabolome of kidney grafts is influenced by the donor ’s type in both human and pre-clinical studies and could be correlated with DGF in the human DBD cohort. Thus, metabolomic analysis of perfusate applied prior to KTx may represent a new predictive tool for clinicians in a more personalized management of DGF. Moreover, our data paves the way to bet ter understand the impact of donor’s types on the biochemical events occurring between death and the hypothermic storage. (Source: Metabolomics)
Source: Metabolomics - March 9, 2024 Category: Biology Source Type: research

Gut metabolic changes during pregnancy reveal the importance of gastrointestinal region in sample collection
ConclusionsThe regional variation observed in the gut metabolome are likely due to the microbial and physiological differences between the different parts of the intestines. Additionally, pregnancy impacts the fecal metabolome, which may be due to evolving needs of both the dam and fetus. (Source: Metabolomics)
Source: Metabolomics - March 9, 2024 Category: Biology Source Type: research

Clinical advances in analytical profiling of signature lipids: implications for severe non-communicable and neurodegenerative diseases
AbstractBackgroundLipids play key roles in numerous biological processes, including energy storage, cell membrane structure, signaling, immune responses, and homeostasis, making lipidomics a vital branch of metabolomics that analyzes and characterizes a wide range of lipid classes. Addressing the complex etiology, age-related risk, progression, inflammation, and research overlap in conditions like Alzheimer's  Disease, Parkinson’s Disease, Cardiovascular Diseases, and Cancer poses significant challenges in the quest for effective therapeutic targets, improved diagnostic markers, and advanced treatments. Mass spectrometr...
Source: Metabolomics - March 8, 2024 Category: Biology Source Type: research

Circulating N-lactoyl-amino acids and N-formyl-methionine reflect mitochondrial dysfunction and predict mortality in septic shock
ConclusionFuture studies which validate the measurement of lac-AAs and f-Met in conjunction with lactate could define a sepsis subtype characterized by mitochondrial dysfunction. (Source: Metabolomics)
Source: Metabolomics - March 6, 2024 Category: Biology Source Type: research