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103 records returned

Overexpression and characterization of medium-chain-length polyhydroxyalkanoate granule bound polymerases from Pseudomonas putida GPo1Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The kinetic properties and substrate ranges were determined for both granule bound polymerases. PhaC1 and PhaC2 exhibited different characteristics in granule release and activity profiles, suggesting that there are substantial functional differences between granule bound PhaC1 and PhaC2. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - November 19, 2009 Category: Microbiology Authors: Qun RenGuy de RooBernard WitholtManfred ZinnLinda Thony-Meyer Source Type: journals

Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This study is the first report on the cloning and expression of the thermostable mannan endo-1,4-beta-mannosidase from A. niger in Pichia pastoris. The efficient expression and ease of purification will significantly decrease the production costs of this enzyme. Taking advantage of its acidic pH optimum and high thermostability, this recombinant beta-mannanase will be valuable in various biotechnological applications. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - November 13, 2009 Category: Microbiology Authors: Do Bien-CoungDang Thi-ThuJean-Guy BerrinDietmar HaltrichTo Kim-AnhJean-Claude SigoillotMontarop Yamabhai Source Type: journals

High yield recombinant thermostable alpha-amylase production using an improved Bacillus licheniformis systemEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This production level of BLA by B. licheniformis CBBD302(pHY-amyL) is amongst the highest levels in Gram-positive bacteria reported so far. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 31, 2009 Category: Microbiology Authors: Dandan NiuZhirui ZuoGui-Yang ShiZheng-Xiang Wang Source Type: journals

Engineering an efficient secretion of leech carboxypeptidase inhibitor in Escherichia coliEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: These results support the hypothesis that heterologous secreted proteins require proper coupling between translation and translocation for optimal high-level production in E. coli. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 29, 2009 Category: Microbiology Authors: Juan-Miguel PuertasJean-Michel Betton Source Type: journals

Yeast cell factory: fishing for the best one or engineering it?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
When today scientists and bioprocess engineers look at a Microbial Cell Factory for the production of a protein or a metabolite of commercial or research interest, they think at the microorganism of choice first from a (i) molecular, then from a (ii) metabolic and finally from a (iii) process point of view. Analyses and manipulations of the pathway(s) involved in the synthesis of the desired product and how this pathway(s) interacts with the overall cell functions and activities are indeed steps required to obtain high yield of the product (g of compound per g of substrate), high production (g/l) and high productivity (g/l...
Source: Microbial Cell Factories - October 11, 2009 Category: Microbiology Authors: Danilo PorroPaola Branduardi Source Type: journals

Expression of active human sialyltransferase ST6GalNAcI in Escherichia coliEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Background: The presence of terminal, surface-exposed sialic acid moieties can greatly enhance the in vivo half-life of glycosylated biopharmaceuticals and improve their therapeutic efficacy. Complete and homogeneous sialylation of glycoproteins can be efficiently performed enzymically in vitro but this process requires large amounts of catalytically active sialyltransferases. Furthermore, standard microbial hosts used for large-scale production of recombinant enzymes can only produce small quantities of glycosyltransferases of animal origin, which lack catalytic activity.Results and conclusion. In this work, we have expre...
Source: Microbial Cell Factories - September 29, 2009 Category: Microbiology Authors: Georgios SkretasSean CarrollShawn DeFreesMarc SchwartzKarl JohnsonGeorge Georgiou Source Type: journals

Increased expression of the oxidative pentose phosphate pathway and gluconeogenesis in anaerobically growing xylose-utilizing Saccharomyces cerevisiaeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Co-factor imbalance in the initial twp steps of xylose utilization may reduce ethanol productivity by increasing the need for NADP+ reduction and consequently increase reverse flux in glycolysis. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - September 23, 2009 Category: Microbiology Authors: David RunquistBarbel Hahn-HagerdalMaurizio Bettiga Source Type: journals

Surface display of heterologous proteins in Bacillus thuringiensis using a peptidoglycan hydrolase anchorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Mbg can be a functional anchor protein to target different heterologous proteins onto the surface of B. thuringiensis cells. Since the LysM domain appears to be universal in Gram-positive bacteria, the strategy presented here could be applicable in other bacteria for developing this type of system. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - September 15, 2009 Category: Microbiology Authors: Xiaohu ShaoMengtian JiangZiniu YuHao CaiLin Li Source Type: journals

Complete PHB mobilization in Escherichia coli enhances the stress tolerance: a potential biotechnological applicationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This engineered E. coli with PHB mobilization has a potential biotechnological application as immobilized cell factories for biocatalysis and biotransformation. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - August 30, 2009 Category: Microbiology Authors: Qian WangHongmin YuYongzhen XiaZhen KangQingsheng Qi Source Type: journals

Yeast artificial chromosomes employed for random assembly of biosynthetic pathways and production of diverse compounds in Saccharomyces cerevisiaeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The technology has many potential uses but is particularly suited for generating high numbers of structurally diverse compounds, some of which may not be amenable to chemical synthesis, thus greatly facilitating access to a huge chemical space in the search for new commercially interesting compounds (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - August 12, 2009 Category: Microbiology Authors: Michael NeasbySoren NielsenCurt NielsenTrine GreenThomas TangeErnesto SimonPhilipp KnechtleAnders HanssonMarkus SchwabOlca TitizChristophe FollyRoberto ArchilaMilena MaverStephan Sint FietThiamo BoussemghouneMichael JanesA S Sathish KumarShailendra Sonkar Source Type: journals

Comparisons of optically monitored small-scale stirred tank vessels to optically controlled disposable bag bioreactorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Similar oxygen delivery rates were achieved in both systems, leading to comparable culture performance (growth and mAb production) across scales and mode of mixing. HTBR model was most fitting when neither system was pH-controlled, providing an information-rich alternative to typically non-monitored mL-scale platforms. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - August 4, 2009 Category: Microbiology Authors: Michael HansonKurt BrorsonAntonio MoreiraGovind Rao Source Type: journals

Development and Experimental Verification of a Genome-Scale Metabolic Model for Corynebacterium glutamicumEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The genome-scale metabolic model provides useful information for the evaluation of the metabolic capabilities and prediction of the metabolic characteristics of C. glutamicum. This can form a basis for the in silico design of C. glutamicum metabolic networks for improved bioproduction of desirable metabolites. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - August 2, 2009 Category: Microbiology Authors: Yohei ShinfukuNatee SorpitipornMasahiro SonoChikara FurusawaTakashi HirasawaHiroshi Shimizu Source Type: journals

Rare codon content affects the solubility of recombinant proteins in a codon bias-adjusted Escherichia coli strainEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our results show that the expression of heterologous proteins coded by high RIL codon content coding sequences in a codon bias-adjusted strain is detrimental for their solubility. Our data support the hypothesis that the possible elimination of translational pauses that increase translation rate leads to protein misfolding and aggregation. This stresses the importance of strain selection according to codon content in any scheme where a large amount of biologically active product is desirable. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - July 23, 2009 Category: Microbiology Authors: German RosanoEduardo Ceccarelli Source Type: journals

A novel bacterial isolate Stenotrophomonas maltophilia as living factory for synthesis of gold nanoparticlesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The process of synthesis of well-dispersed nanoparticles using a noble microorganism isolated from the gold enriched soil sample has been reported in this study, leading to the development of an easy bioprocess for synthesis of GNPs. This is the first study in which an extensive characterisation of the indigenous bacterium isolated from the actual gold enriched soil was conducted. Promising mechanism for the biosynthesis of GNPs by the strain and their stabilization via charge capping suggested involvement of NADPH-dependent reductase enzyme that reduces Au3+ to Au0 through electron shuttle enzymatic metal red...
Source: Microbial Cell Factories - July 19, 2009 Category: Microbiology Authors: Yogesh NangiaNishima WangooNisha GoyalG ShekhawatC Raman Suri Source Type: journals

Bacillus subtilis as potential producer for polyhydroxyalkanoatesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Polyhydroxyalkanoates (PHAs) are biodegradable polymers produced by microbes to overcome environmental stress. Commercial production of PHAs is limited by the high cost of production compared to conventional plastics. Another hindrance is the brittle nature and low strength of polyhydroxybutyrate (PHB), the most widely studied PHA. The needs are to produce PHAs, which have better elastomeric properties suitable for biomedical applications, preferably from inexpensive renewable sources to reduce cost. Certain unique properties of Bacillus subtilis such as lack of the toxic lipo-polysaccharides, expression of self-lysing gen...
Source: Microbial Cell Factories - July 19, 2009 Category: Microbiology Authors: Mamtesh SinghSanjay PatelVipin Kalia Source Type: journals

Interactive visualization of clusters in microarray data: an efficient tool for improved metabolic analysis of E. coliEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: It was shown that gcExplorer is a very helpful tool to gain a general overview of microarrayexperiments. Interesting gene expression patterns can easily be found, compared among different experimentsand combined with information about gene function from publicly available databases. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - July 14, 2009 Category: Microbiology Authors: Theresa ScharlGerald StriednerFlorentina PoetschacherFriedrich LeischKarl Bayer Source Type: journals

Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase systemEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This study demonstrates that the identification of new P450s capable of producing valuable compounds can basically be achieved by screening of recombinant P450 libraries. The biphasic reaction system described in this work presents an attractive way for the production of (+)-nootkatone (4), as it is safe and can easily be controlled and scaled up. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - July 9, 2009 Category: Microbiology Authors: Marco GirhardKazuhiro MachidaMasashi ItohRolf SchmidAkira ArisawaVlada Urlacher Source Type: journals

Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regimeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We demonstrate how a rational, stepwise approach to recombinant protein production screens can reduce process development time. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 30, 2009 Category: Microbiology Authors: William HolmesRichard DarbyMartin WilksRodney SmithRoslyn Bill Source Type: journals

Molecular cloning and expression of a novel trehalose synthase gene from Enterobacter hormaecheiEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: In this study, several TreS producing strains were screened from a source of soil bacteria. The characterization of the recombinant TreS of Enterobacter hormaechei suggested its potential application. Consequently, a strategy for isolation of TreS producing strains and cloning of novel treS genes from natural sources was demonstrated. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 11, 2009 Category: Microbiology Authors: Ming YueXiu Li WuWei Na GongHong Biao Ding Source Type: journals

PimT, an amino-acid exporter controls polyene production via secretion of the quorum-sensing pimaricin-inducer PI-factor in Streptomyces natalensisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This report describes the involvement of an amino acid exporter (encoded by pimT in the vicinity of the pimaricin cluster) in modulating the expression of antibiotic biosynthetic genes via secretion of the quorum-sensing pimaricin-inducer PI-factor. The discovery of the participation of amino acid exporters in a signal transduction cascade for the production of polyene macrolides is unexpected, and represents an important step forward towards understanding the regulatory network for polyene regulation. Additionally, this finding constitutes the first detailed characterization of an amino-acid exporter in an Act...
Source: Microbial Cell Factories - June 8, 2009 Category: Microbiology Authors: Claudia VicenteJavier Santos-AberturasSusana GuerraTamara PayeroJuan MartinJesus Aparicio Source Type: journals

Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasmEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Taking together physiological and biochemical evidence, our work indicates that the E. coli envelope can sense protein over-expression in the cytoplasm and react by modulating the abundance of some membrane proteins, with possible consequences on the membrane traffic of small solutes, i.e. nutrients, drugs and metabolites. Such a response seems to be independent on the nature of the protein being over-expressed. On the other hand both our data reported herein and previous results indicate that membrane lipids may act as a second stress sensor responsive to the aggregation state of the recombinant protein and f...
Source: Microbial Cell Factories - June 5, 2009 Category: Microbiology Authors: Riccardo VillaMarina LottiPietro Gatti-Lafranconi Source Type: journals

Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regenerationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We have achieved a more than 500-fold improvement in the expression of soluble T. maritima 6PGDH in E. coli, characterized its basic biochemical properties, and demonstrated its applicability for NADPH regeneration by a new enzyme cocktail. The methodology for over-expression and simple purification of this thermostable protein would be useful for the production of other thermostable proteins in E. coli. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 4, 2009 Category: Microbiology Authors: Yiran WangY.-H. Percival Zhang Source Type: journals

Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter platesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The BioLector technology is a very useful tool to perform quantitative microfermentations under engineered reaction conditions. With this technique, specific yields and rates can be directly deduced from online biomass and product concentrations, which is superior to existing technologies such as microplate readers or optode-based cultivation systems. In particular, applications with strong demand on high-throughput such as clone and media screening and systems biology can benefit from its simple handling, the high quantitative information content and its capacity of automation. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 4, 2009 Category: Microbiology Authors: Frank KensyEmerson ZangChristian FaulhammerRung-Kai TanJochen Buchs Source Type: journals

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastorisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This work highlights significant advantages of using the P. pastoris system with glucose based expression and fermentation strategies. As only few proteins and no proteases are actually secreted on glucose, it becomes evident that cell lysis is the relevant cause of proteolytic degradation of secreted proteins. The endowment with hexose transporters, dominantly of the high affinity type, limits glucose uptake rates and thus overflow metabolism as observed in S. cerevisiae. The presence of 4 genes for glycerol transporters explains the high specific growth rates on this substrate and underlines the suitability ...
Source: Microbial Cell Factories - June 2, 2009 Category: Microbiology Authors: Diethard MattanovichAlexandra GrafJohannes StadlmannMartin DragositsAndreas RedlMichael MaurerMartin KleinheinzMichael SauerFriedrich AltmannBrigitte Gasser Source Type: journals

Heme and menaquinone induced electron transport in lactic acid bacteriaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We propose that the cyd-genes were present in the common ancestor of lactic acid bacteria, and that multiple gene-loss events best explains the observed distribution of these genes among the species. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 29, 2009 Category: Microbiology Authors: Rob BrooijmansBart SmitFilipe SantosJan van RielWillem de VosJeroen Hugenholtz Source Type: journals

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coliEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This article reviews the available strategies conceived for exploiting the bacteria physiological mechanisms in order to produce disulfide-bonded proteins with proper folding. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 14, 2009 Category: Microbiology Authors: Ario de Marco Source Type: journals

Identification of potentially safe promising fungal cell factories for the production of polyketide natural food colorants using chemotaxonomic rationaleEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The present work brought out that the use of chemotaxonomic tools and a priori knowledge of fungal extrolites is a rational approach towards selection of fungal polyketide pigment producers considering the enormous chemical diversity and biodiversity of ascomycetous fungi. This could very well serve a useful piece of information for the selection of potentially safe fungal cell factories not only for polyketide pigments but also for the other industrially important polyketides, the molecular and genetic basis for the biosynthesis of which has not yet been examined in detail. In addition, 4 out of the 10 chemot...
Source: Microbial Cell Factories - April 27, 2009 Category: Microbiology Authors: Sameer AS Mapari, Anne S Meyer, Ulf Thrane and Jens C Frisvad Source Type: journals

Homolactic fermentation from glucose and cellobiose using Bacillus subtilisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This study shows that by taking advantage of the cellobiose utilization capability and osmotic stress high resistance of B. subtilis, a robust process for L-lactate production can be developed. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 21, 2009 Category: Microbiology Authors: Susana Romero-Garcia, Claudia Hernandez-Bustos, Enrique Merino, Guillermo Gosset and Alfredo Martinez Source Type: journals

Process development in Hansenula polymorpha and Arxula adeninivorans, a re-assessmentEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A range of industrial H. polymorpha-based processes exist, most of them for the production of pharmaceuticals. The established industrial processes lean on the use of promoters derived from MOX and FMD, genes of the methanol metabolism pathway. In Hansenula polymorpha these promoters are de-repressed upon depletion of a range of carbon sources like glucose and glycerol instead of being induced by methanol as reported for other methylotrophs. Due to these characteristics screening and fermentation modes have been defined for strains harbouring such expression control elements that lean on a limited supplementation of glycer...
Source: Microbial Cell Factories - April 15, 2009 Category: Microbiology Authors: Christoph Stockmann, Marco Scheidle, Doris Klee, Barbara Dittrich, Armin Merckelbach, Grit Hehmann, Georg Melmer, Jochen Buchs, Hyun Ah Kang and Gerd Gellissen Source Type: journals

Optimization of physical factors affecting the production of thermostable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain RandEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Strain Rand has been found to be able to secrete extra-cellular thermostable organic solvent-tolerant protease into the culture medium. The protease exhibited a remarkable stability towards temperature and organic solvent. This unique property makes it attractive and useful to be used in industrial applications. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 9, 2009 Category: Microbiology Authors: Randa A. Abusham, Raja Noor Zaliha RA. Rahman, Abu Bakar Salleh and Mahiran Basri Source Type: journals

Medical bioremediation of age-related diseasesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Catabolic insufficiency in humans leads to the gradual accumulation of a number of pathogenic compounds associated with age-related diseases, including atherosclerosis, Alzheimer's disease, and macular degeneration. Removal of these compounds is a widely researched therapeutic option, but the use of antibodies and endogenous enzymes has failed to produce effective treatments, and may pose risks to cellular homeostasis. Another alternative is "medical bioremediation," or the use of microbial enzymes to augment missing catabolic functions. The genetic diversity in most natural environments provides a resource that can be min...
Source: Microbial Cell Factories - April 9, 2009 Category: Microbiology Authors: Jacques M Mathieu, John Schloendorn, Bruce E Rittmann and Pedro JJ Alvarez Source Type: journals

Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coliEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This work constitutes the first example of a microbial system for the environmentally-compatible synthesis of anthranilate generated by metabolic engineering. The results presented here, including the characterization of mutation in the trpD gene from strain W3110 trpD9923 and the development of a fermentation strategy, establish a step forward towards the future improvement of a sustainable process for anthranilate production. In addition, the present work provides very useful data regarding the positive and negative consequences of the evaluated metabolic engineering strategies. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 2, 2009 Category: Microbiology Authors: Victor E. Balderas-Hernandez, Andrea Sabido-Ramos, Patricia Silva, Natividad Cabrera-Valladares, Georgina Hernandez-Chavez, Jose L. Baez-Viveros, Alfredo Martinez, Francisco Bolivar and Guillermo Gosset Source Type: journals

Phenotypes and gene expression profiles of Saccharopolyspora erythraea rifampicin-resistant (rif) mutants affected in erythromycin productionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we have used rifampicin selection as a strategy to genetically improve the erythromycin producer Saccharopolyspora erythraea. Results: Spontaneous rifampicin-resistant (rif) mutants were isolated from the parental strain NRRL2338 and two rif mutations mapping within rpoB, S444F and Q426R, were characterized. With respect to the parental strain, S444F mutants exhibited higher respiratory performance and up to four-fold higher final erythromycin yields; in contrast, Q426R mutants were slow-growing, developmental-defective and severely impaired in erythromycin production. DNA microarray analysis demonstrated tha...
Source: Microbial Cell Factories - March 30, 2009 Category: Microbiology Authors: Elisabetta Carata, Clelia Peano, Salvatore M Tredici, Francesco Ferrari, Adelfia Talà, Giorgio Corti, Silvio Bicciato, Gianluca De Bellis and Pietro Alifano Source Type: journals

Microbial factories for recombinant pharmaceuticalsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Most of the hosts used to produce the 151 recombinant pharmaceuticals so far approved for human use by the Food and Drug Administration (FDA) and/or by the European Medicines Agency (EMEA) are microbial cells, either bacteria or yeast. This fact indicates that despite the diverse bottlenecks and obstacles that microbial systems pose to the efficient production of functional mammalian proteins, namely lack or unconventional post-translational modifications, proteolytic instability, poor solubility and activation of cell stress responses, among others, they represent convenient and powerful tools for recombinant protein prod...
Source: Microbial Cell Factories - March 24, 2009 Category: Microbiology Authors: Neus Ferrer-Miralles, Joan Domingo-Espin, Jose Luis Corchero, Esther Vazquez and Antonio Villaverde Source Type: journals

Synthesis of rhamnolipid biosurfactant and mode of hexadecane uptake by Pseudomonas speciesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This study throws more light on the uptake mechanism of hydrocarbon by Pseudomonas aeruginosa. We report here a new and exciting line of research for hydrocarbon uptake involving internalization of biosurfactant covered hydrocarbon inside cell for subsequent breakdown. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - March 11, 2009 Category: Microbiology Authors: Swaranjit Singh S Cameotra and Pooja Singh Source Type: journals

Bioreactor mixing efficiency modulates the activity of a prpoS::GFP reporter gene in E. coliEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The generation of extracellular perturbations (in the present case, at the level of the sugar concentration and the dissolved oxygen level) has led to a drop at the level of the rpoS expression level. This drop has to be attributed to a segregation phenomenon in microbial population, with a major sub-population exhibiting a low expression level and a minor sub-population keeping its initial elevated expression level. The intensity of the segregation, as well as its time of appearance during the culture can be related to the bioreactor mixing efficiency. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 25, 2009 Category: Microbiology Authors: Frank Delvigne, Mathieu Boxus, Sophie Ingels and Philippe Thonart Source Type: journals

Bioreactor mixing efficiency modulates the activity of a prpoS::GFP reporter gene in E. coliEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion The generation of extracellular perturbations (in our case, at the level of the sugar concentration and the dissolved oxygen level) has led to a drop at the level of the rpoS expression level. This drop has been mainly attributed to a segregation phenomenon at the level of the microbial population, with a major sub-population exhibiting a low expression level and a minor sub-population keeping its initial elevated expression level. The intensity of the segregation, as well as its time of appearance during the culture can be related to the bioreactor mixing efficiency. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 25, 2009 Category: Microbiology Authors: Frank Delvigne, Mathieu Boxus, Sophie Ingels and Philippe Thonart Source Type: journals

Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: In comparison to the highest yields reported so far, our simple cultivation process resulted in an ~7 fold enhancement in total HBsAg production with more than 30% of soluble protein competent for assembly into VLPs. This work opens up the possibility of significantly reducing the cost of vaccine production with implications for expanding hepatitis B vaccination in resource-poor countries. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 10, 2009 Category: Microbiology Authors: Chandrasekhar Gurramkonda, Ahmad Adnan, Thomas Gäbel, Heinrich Lünsdorf, Anton Ross, Satish Kumar Nemani, Sathyamangalam Swaminathan, Navin Khanna and Ursula Rinas Source Type: journals

Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: In comparison to the highest yields reported so far, our simple cultivation process resulted in an ~7 fold enhancement in total HBsAg production with more than 30% of soluble protein competent for assembly into VLPs. This work opens up the possibility of significantly reducing the cost of vaccine production with implications for expanding hepatitis B vaccination in resource-poor countries. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 10, 2009 Category: Microbiology Authors: Chandrasekhar Gurramkonda, Ahmad Adnan, Thomas Gabel, Heinrich Lunsdorf, Anton Ross, Satish Kumar Nemani, Sathyamangalam Swaminathan, Navin Khanna and Ursula Rinas Source Type: journals

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yieldEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This work demonstrates the power of a rational approach to recombinant protein production by using the results of transcriptome analysis to engineer improved strains, thereby revealing the underlying biological events involved. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 29, 2009 Category: Microbiology Authors: Nicklas Bonander, Richard AJ Darby, Ljuban Grgic, Nagamani Bora, Jikai Wen, Saverio Brogna, David R Poyner, Michael AA O'Neill and Roslyn M Bill Source Type: journals

Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: The LARDs or whole TliA were attached to C-termini of model proteins and enabled the export of the model proteins such as GFP and EGF in E. coli supplemented with ABC transporter. These results open the possibility for the extracellular production of recombinant proteins in Pseudomonas using LARDs or TliA as a C-terminal signal sequence. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 29, 2009 Category: Microbiology Authors: Chan Woo Chung, Jinsun You, Kyeongyeon Kim, Yuseok Moon, Hoeon Kim and Jung Hoon Ahn Source Type: journals

Pediocins: The bacteriocins of Pediococci. Sources, production, properties and applicationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Class IIa bacteriocins from lactic acid bacteria are small, cationic proteins with antilisterial activity. Within this class, the pediocins are those bacteriocins that share a highly conserved hydrophilic and charged N-terminal part harboring the consensus sequence -YGNGV- and a more variable hydrophobic and /or amphiphilic C-terminal part. Several pediocins have been isolated and characterized. Despite the structural similarities, their molecular weight varies, as well as their spectrum of antimicrobial activity. They exhibit important technological properties, e.g. thermostability and retaining of activity at a wide pH r...
Source: Microbial Cell Factories - January 8, 2009 Category: Microbiology Authors: Maria Papagianni and Sofia Anastasiadou Source Type: journals

Learning about protein solubility from bacterial inclusion bodiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The progressive solving of the conformation of aggregated proteins and the conceptual understanding of the biology of inclusion bodies in recombinant bacteria is providing exciting insights on protein folding and quality. Interestingly, newest data also show an unexpected functional and structural complexity of soluble recombinant protein species and picture the whole bacterial cell factory scenario as more intricate than formerly believed. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 8, 2009 Category: Microbiology Authors: Monica Martinez-Alonso, Nuria Gonzalez-Montalban, Elena Garcia-Fruitos and Antonio Villaverde Source Type: journals

Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine productionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This result demonstrates that the elimination of genes unnecessary for cell growth can increase the productivity of an industrial strain, most likely by reducing the metabolic burden and improving the metabolic efficiency of cells. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 7, 2009 Category: Microbiology Authors: Jun Hyoung Lee, Bong Hyun Sung, Mi Sun Kim, Frederick R Blattner, Byoung Hoon Yoon, Jung Hoe Kim and Sun Chang Kim Source Type: journals

Molecular cloning and characterization of a novel pyrethroid-hydrolyzing esterase originating from the MetagenomeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: A novel pyrethroid-hydrolyzing esterase gene was successfully cloned using metagenomic DNA combined with activity-based functional screening from soil, the broader substrate specificities and higher activity of the pyrethroid-hydrolyzing esterase (Pye3) make it an ideal candidate for in situ for detoxification of pyrethroids where they cause environmental contamination problems. Consequently, metagenomic DNA clone library offers possibilities to discover novel bio-molecules through the expression of genes from uncultivated bacteria. (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - December 30, 2008 Category: Microbiology Authors: Gang Li, Kui Wang and Yu H Liu Source Type: journals

Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanolEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Background: Increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol. Results and conclusionsSaccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (S. cerevisiae, Escherichia coli, Clostridium beijerinckii, and Ralstonia eutropha) were substituted for the Clostridial enzymes and their effect on n-butanol production was compared. By choosing the appropriate isozymes, we were able to improve production of n-butanol ten-fold t...
Source: Microbial Cell Factories - December 3, 2008 Category: Microbiology Authors: Eric J. Steen, Rossana Chan, Nilu Prasad, Samuel Myers, Christopher J. Petzold, Alyssa Redding, Mario Ouellet and Jay D. Keasling Source Type: journals

The scientific impact of microbial cell factoriesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Microbial Cell Factories was launched in 2002 under an Open Access policy, to cover a gap in the current offer of the scientific literature in Biotechnology and Applied Microbiology areas. The microbial cell factory concept, although present as a side topic within the scope of many journals in the field, deserves a specific attention as a particular, well defined issue in the microbial production and transformation of biotechnologically relevant substances. Intriguingly, the Cell Factory concept stresses the relevance of host cell genetics and metabolism in the context of the production process, and focus on the physiologi...
Source: Microbial Cell Factories - December 1, 2008 Category: Microbiology Authors: Maurilio De Felice, Diethard Mattanovich, Maria Papagianni, Grzegorz Wegrzyn and Antonio Villaverde Source Type: journals

Engineering inclusion bodies for non denaturing extraction of functional proteinsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This study shows that the presence of biologically active proteins inside IBs is more general than usually believed. A large amount of properly folded protein is trapped inside IBs prepared at lower temperatures. This protein can be released from IBs with mild detergents under non-denaturing conditions. Therefore, the active protein can be obtained from such IBs without any renaturation procedure. This is of great importance for the biopharmaceutical industry. Furthermore, such IBs composed of active proteins could also be used as pure nanoparticles in diagnostics, as biocatalysts in enzymatic processes, or eve...
Source: Microbial Cell Factories - December 1, 2008 Category: Microbiology Authors: Spela Peternel, Joze Grdadolnik, Vladka Gaberc-Porekar and Radovan Komel Source Type: journals

Norvaline is accumulated after a down-shift of oxygen in Escherichia coli W3110Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: Norvaline synthesis has been so far mainly related to an imbalance of the synthesis of the branched chain amino acids under conditions were pyruvate level is high. Here we show that simply a downshift of oxygen is sufficient to cause norvaline accumulation at a high glucose concentration as a consequence of the accumulation of pyruvate and its direct chain elongation over α-ketobutyrate and α-ketovalerate.Although the flux to norvaline is low, millimolar concentrations are accumulated in the cultivation broth, which is far above the level which has been discussed for being relevant for misincorporation of nor...
Source: Microbial Cell Factories - October 21, 2008 Category: Microbiology Authors: Jaakko Soini, Christina Falschlehner, Christina Liedert, Jörg Bernhardt, Jussi Vuoristo and Peter Neubauer Source Type: journals