Characterization and optimization of exopolysaccharide extracted from a newly isolated halotolerant cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1 with antiviral activity
This study aimed to identify and optimize the conditions for exopolysaccharide (EPS) production from a newly i... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 22, 2024 Category: Microbiology Authors: Mabroka H. Saad, Nagwa M. Sidkey and Esmail M. El-Fakharany Tags: Research Source Type: research
Combinatorial metabolic engineering of Bacillus subtilis enables the efficient biosynthesis of isoquercitrin from quercetin
Isoquercitrin (quercetin-3-O- β-D-glucopyranoside) has exhibited promising therapeutic potentials as cardioprotective, anti-diabetic, anti-cancer, and anti-viral agents. However, its structural complexity and l... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 20, 2024 Category: Microbiology Authors: Tengfei Niu, Chaokang Huang, Rufeng Wang, Li Yang, Shujuan Zhao and Zhengtao Wang Tags: Research Source Type: research
PySupercharge: a python algorithm for enabling ABC transporter bacterial secretion of all proteins through amino acid mutation
The process of producing proteins in bacterial systems and secreting them through ATP-binding cassette (ABC) transporters is an area that has been actively researched and used due to its high protein productio... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 20, 2024 Category: Microbiology Authors: Yerin Kim, Danny Kim, Nguyen-Mihn Hieu, Hyunjong Byun and Jung Hoon Ahn Tags: Methodology Source Type: research
Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection
Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes. The clonal variability in gene copy numbers, integration loci a... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 20, 2024 Category: Microbiology Authors: Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder and Xavier Garcia-Ortega Tags: Research Source Type: research
Engineering Streptomyces sp. CPCC 204095 for the targeted high-level production of isatropolone A by elucidating its pathway-specific regulatory mechanism
Isatropolone A and C, produced by Streptomyces sp. CPCC 204095, belong to an unusual class of non-benzenoid aromatic compounds and contain a rare seven-membered ring structure. Isatropolone A exhibits potent acti... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 16, 2024 Category: Microbiology Authors: Cong Zhang, Qianqian Xu, Jie Fu, Linzhuan Wu, Yihong Li, Yuan Lu, Yuanyuan Shi, Hongmin Sun, Xingxing Li, Lifei Wang and Bin Hong Tags: Research Source Type: research
Optimizing microbioreactor cultivation strategies for Trichoderma reesei: from batch to fed-batch operations
Filamentous fungi have long been recognized for their exceptional enzyme production capabilities. Among these, Trichoderma reesei has emerged as a key producer of various industrially relevant enzymes and is part... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 15, 2024 Category: Microbiology Authors: Katja Rohr, Lisa Gremm, Bertram Geinitz, Etienne Jourdier, Wolfgang Wiechert, Fadhel Ben Chaabane and Marco Oldiges Tags: Research Source Type: research
Unlocking the genome of the non-sourdough Kazachstania humilis MAW1: insights into inhibitory factors and phenotypic properties
Ascomycetous budding yeasts are ubiquitous environmental microorganisms important in food production and medicine. Due to recent intensive genomic research, the taxonomy of yeast is becoming more organized bas... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 15, 2024 Category: Microbiology Authors: Damian Mielecki, Anna Detman, Tamara Aleksandrzak-Piekarczyk, Ma łgorzata Widomska, Aleksandra Chojnacka, Anna Stachurska-Skrodzka, Paulina Walczak, Elżbieta Grzesiuk and Anna Sikora Tags: Research Source Type: research
Deciphering antifungal and antibiofilm mechanisms of isobavachalcone against Cryptococcus neoformans through RNA-seq and functional analyses
Cryptococcus neoformans has been designated as critical fungal pathogens by the World Health Organization, mainly due to limited treatment options and the prevalence of antifungal resistance. Consequently, the ut... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 12, 2024 Category: Microbiology Authors: Weidong Qian, Jiaxing Lu, Chang Gao, Qiming Liu, Yongdong Li, Qiao Zeng, Jian Zhang, Ting Wang and Si Chen Tags: Research Source Type: research
Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly
Microbial cell surface display technology allows immobilizing proteins on the cell surface by fusing them to anchoring motifs, thereby endowing the cells with diverse functionalities. However, the assessment o... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 12, 2024 Category: Microbiology Authors: Li Zhang, Ling Tan, Meizi Liu, Yunhong Chen, Yu Yang, Yanfei Zhang and Guoping Zhao Tags: Research Source Type: research
Green ecofriendly enhancement of cellulase productivity using agricultural wastes by Aspergillus terreus MN901491: statistical designs and detergent ability on cotton fabrics
Cellulase is considered a group member of the hydrolytic enzymes, responsible for catalyzing the hydrolysis of cellulose and has various industrial applications. Agricultural wastes are used as an inexpensive ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 12, 2024 Category: Microbiology Authors: Mohamed A.A. Abdella, Nehad E. Ahmed and Mohamed S. Hasanin Tags: Research Source Type: research
Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry
The wasabi receptor, also known as the Transient Receptor Potential Ankyrin 1 (TRPA1) ion channel, is a potential target for development of repellents for insects, like the pine weevil (Hylobius abietis) feeding ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 12, 2024 Category: Microbiology Authors: Balder Werin, Wilhelm Hansson Wennersten, Robin Olsson, Oliwia Ko łodziejczyk, Martin N. Andersson, Magnus Carlquist and Urban Johanson Tags: Research Source Type: research
Valorisation of cotton post-industrial textile waste into lactic acid: chemo-mechanical pretreatment, separate hydrolysis and fermentation using engineered yeast
The textile industry has several negative impacts, mainly because it is based on a linear business model that depletes natural resources and produces excessive amounts of waste. Globally, about 75% of textile ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 10, 2024 Category: Microbiology Authors: Marta Simonetti, Pietro Butti, Raffaella Desir é Di Lorenzo, Valeria Mapelli and Paola Branduardi Tags: Research Source Type: research
Droplet-based microfluidic platform for detecting agonistic peptides that are self-secreted by yeast expressing a G-protein-coupled receptor
Single-cell droplet microfluidics is an important platform for high-throughput analyses and screening because it provides an independent and compartmentalized microenvironment for reaction or cultivation by co... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 9, 2024 Category: Microbiology Authors: Ririka Asama, Cher J. S. Liu, Masahiro Tominaga, Yu-Ru Cheng, Yasuyuki Nakamura, Akihiko Kondo, Hsiang-Yu Wang and Jun Ishii Tags: Research Source Type: research
Pregnenolone and progesterone production from natural sterols using recombinant strain of Mycolicibacterium smegmatis mc2 155 expressing mammalian steroidogenesis system
Pregnenolone and progesterone are the life-important steroid hormones regulating essential vital functions in mammals, and widely used in different fields of medicine. Microbiological production of these compo... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 9, 2024 Category: Microbiology Authors: Mikhail Karpov, Nicolai Strizhov, Ludmila Novikova, Tatyana Lobastova, Sergey Khomutov, Andrei Shutov, Alexey Kazantsev and Marina Donova Tags: Research Source Type: research
Characterization of a pleiotropic regulator MtrA in Streptomyces avermitilis controlling avermectin production and morphological differentiation
The macrolide antibiotic avermectin, a natural product derived from Streptomyces avermitilis, finds extensive applications in agriculture, animal husbandry and medicine. The mtrA (sav_5063) gene functions as a tr... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 8, 2024 Category: Microbiology Authors: Jinpin Tian, Yue Li, Chuanbo Zhang, Jianyu Su and Wenyu Lu Tags: Research Source Type: research
