Analysis of axial scanning range and magnification variation in wide ‐field microscope for measurement using an electrically tunable lens
AbstractInserting an electrically tunable lens (ETL), such as liquid lens or tunable acoustic gradient lens, into a microscope can enable fast axial scanning, autofocusing, and extended depth of field. However, placing the ETL at different positions has different influences on image quality. Specially, in a wide ‐field microscope for measurement, the magnification has to be constant when introducing an ETL, otherwise it will affect measurement accuracy. To determine the best position of ETL, axial scanning range and magnification variation are quantitatively analyzed and discussed in finite and infinite m icroscopes thro...
Source: Microscopy Research and Technique - November 19, 2018 Category: Laboratory Medicine Authors: Yufu Qu,
Yongbo Hu Tags: RESEARCH ARTICLE Source Type: research
Taxonomic significance of macro and micro ‐morphology of Geranium L. species Using Scanning Electron Microscopy
Macro and micro ‐morphology of different parts of 22 taxaGeranium species comprises sepal, petal, flower indumentum and leaf shape were studied.
Using scanning electron microscopy techniques for the easy and quick identification of plant species.
Mineral level did not change in TAPm group.
Using micro ‐morphological (SEM) characters for the identification and species delimitations. AbstractMacro and micromorphology of different parts that is, sepals, petals, flowers, indumentum, and leaf shape of 22Geranium L. taxa from Iran were studied. These taxa representing the eight sections of the genus as sect.Dissecta, sect.Ge...
Source: Microscopy Research and Technique - November 19, 2018 Category: Laboratory Medicine Authors: Somayeh Esfandani ‐Bozchaloyi,
Wajid Zaman Tags: RESEARCH ARTICLE Source Type: research
Assessment of mold and yeast in some bakery products of Lahore, Pakistan based on LM and SEM
AbstractThe present investigation was designed to throw light on the microbial status of bakery products available in bakeries and supermarkets of Lahore. Different bakery samples such as biscuits, pizza, patties were collected from different localities such as Anar Kali, Chauburji, Faisal Town, Iqbal Town, Model Town, Muslim Town were investigated for mold and yeast using serial dilution technique inoculated over malt extract agar and potato dextrose agar under sterilized conditions. Isolated fungi were namelyAlternaria alternata, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Aspergillus terreus, Curvulari...
Source: Microscopy Research and Technique - November 18, 2018 Category: Laboratory Medicine Authors: Iqra Saeed,
Shabnum Shaheen,
Khadim Hussain,
Muhammad Asaf Khan,
Mehwish Jaffer,
Tariq Mahmood,
Sana Khalid,
Samina Sarwar,
Arifa Tahir,
Farah Khan Tags: RESEARCH ARTICLE Source Type: research
Cover Image
On the cover: The cover image is based on the Research ArticleDifferences between FLIM phasor analyses for data collected with the Becker and Hickl SPC830 card and with the FLIMbox card by Suman Ranjit et al., DOI:https://doi.org/10.1002/jemt.23061. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - November 17, 2018 Category: Laboratory Medicine Authors: Suman Ranjit,
Leonel Malacrida,
Enrico Gratton Tags: COVER IMAGE Source Type: research
Issue Information
Microscopy Research and Technique, Volume 81, Issue 9, Page 921-922, September 2018. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - November 17, 2018 Category: Laboratory Medicine Tags: ISSUE INFORMATION Source Type: research
Introduction to the special section on light sheet fluorescence illumination microscopy
Microscopy Research and Technique, Volume 81, Issue 9, Page 923-923, September 2018. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - November 17, 2018 Category: Laboratory Medicine Authors: Francesca Cella Zanacchi,
Alberto Diaspro Tags: EDITORIAL PREFACE Source Type: research
Diabetic retinopathy detection and classification using hybrid feature set
Local contrast enhancement, adaptive threshold, mathematical morphology methods are used for lesion detection. Multiple machine learning classifiers are utilized to evaluate the performance of proposed approach. The proposed method tested at image and lesion level. AbstractComplicated stages of diabetes are the major cause of Diabetic Retinopathy (DR) and no symptoms appear at the initial stage of DR. At the early stage diagnosis of DR, screening and treatment may reduce vision harm. In this work, an automated technique is applied for detection and classification of DR. A local contrast enhancement method is used on graysc...
Source: Microscopy Research and Technique - November 17, 2018 Category: Laboratory Medicine Authors: Javeria Amin,
Muhammad Sharif,
Amjad Rehman,
Mudassar Raza,
Muhammad Rafiq Mufti Tags: RESEARCH ARTICLE Source Type: research
Fast free of acrylamide clearing tissue (FACT) for clearing, immunolabelling and three ‐dimensional imaging of partridge tissues
AbstractFast free of acrylamide clearing tissue (FACT) is a modified sodium dodecyl sulfate ‐based clearing protocol for the chemical clearing of lipids that completely preserves fluorescent signals in the cleared tissues. The FACT protocol was optimized to image translucent immunostained brain and non‐nervous tissues. For this purpose adult male Chukar partridge (Alectoris chukar) was used as a model. After clearing the tissues, 1 or 2 mm‐thickness sections of tissues were immunolabeled. The paraventricular nucleus in the hypothalamus (2‐mm section) was cleared with FACT, and then was stained with gonadotropin...
Source: Microscopy Research and Technique - November 15, 2018 Category: Laboratory Medicine Authors: Farzad Mohammad Rezazadeh,
Saman Saedi,
Farhad Rahmanifar,
Mohammad Reza Namavar,
Mehdi Dianatpour,
Nader Tanideh,
Amir Akhlaghi,
Ali Niazi,
Ali Arabi Monfared,
Kazuyoshi Tsutsui,
Mohammad Reza Jafarzadeh Shirazi,
Amin Tamadon Tags: RESEARCH ARTICLE Source Type: research
High ‐speed quantitative 3D imaging by dual‐illumination holographic microscopy
AbstractA new blood flow imaging (BFI) technique using digital holography with double illumination of the sample is proposed. We imaged the moving red blood cells (RBCs) using a two microscope objective lenses setup. The setup consists in a larger angle of separation (90 °) between the two illumination beams, allowing a wider angular rotation at good z resolution. Moreover, the setup geometry allows an easier displacement of the sample in all directions. Results show that this technique is able to perform phase‐shifting reconstruction for the two beams at the same time which is more suitable for the future implementa...
Source: Microscopy Research and Technique - November 15, 2018 Category: Laboratory Medicine Authors: Dario Donnarumma,
Nitin Rawat,
Alexey Brodoline Tags: RESEARCH ARTICLE Source Type: research
Image segmentation by graph cut for radiation images of small animal blood vessels
This study focused on finding a suitable algorithm for image segmentation in high‐resolution medical imaging. In this study, we used follow ing four algorithms to segment blood vessel of mouse; interactive graph cuts algorithm, which segments an image using fast min‐cut/max‐flow algorithm to solve global solution, binary partition tree algorithm, which uses an interactive method creating tree nodes to segment an image by using split ting and merging an image, seeded region growing algorithm, which performs segmentation by connecting similar pixel value, simple interactive object extraction, which generates color sign...
Source: Microscopy Research and Technique - November 8, 2018 Category: Laboratory Medicine Authors: Taewan Kim,
Chibum Ahn,
Onseok Lee Tags: RESEARCH ARTICLE Source Type: research
Structural and chemical characterization of rice and potato starch granules using microscopy and spectroscopy
Investigate the changes of size and shape of starch granules (rice and potato) before and after hydrolysis using optical microscope and scanning electron microscope
Chemical and structural properties of starches using Fourier transform infrared and X ‐ray diffraction spectroscopy AbstractStarch is a polysaccharide that plays an important role in our diet and aids in determining the blood glucose levels and is the main source of energy to humans and plants. Starch is broken down by hydrolases which are present in our digestive system. We have used α‐amylase for investigating the rate of hydrolysis of rice and potato st...
Source: Microscopy Research and Technique - November 8, 2018 Category: Laboratory Medicine Authors: Prarthana V. Kowsik,
Nirmal Mazumder Tags: RESEARCH ARTICLE Source Type: research
Saponins enriched in the epidermal layer of Holothuria leucospilota body wall
‐ Electron microscopy and MALDI‐IMS revealed the major compositions and the distributions of saponins inH. leucospilota body wall, particularly in the epidermis. ‐ The epidermal saponins, m/z 1,243.3 and 1,259.3 were the most abundant compounds. AbstractSaponins are secondary metabolites that provide medicinal benefits in controlling body homeostasis and metabolic functions. Sea cucumber has been consumed in many Asian countries due to their health benefits. Active chemicals found in sea cucumber include natural source of saponins which are enriched in their tissues, including the Cuvierian tubules and the body wall....
Source: Microscopy Research and Technique - November 8, 2018 Category: Laboratory Medicine Authors: Morakot Sroyraya,
Wanita Kaewphalug,
Natthinee Anantachoke,
Tanes Poomtong,
Prasert Sobhon,
Atthapol Srimongkol,
Worawit Suphamungmee Tags: RESEARCH ARTICLE Source Type: research
Compare the microscopic characteristics of stems of the 24 Dendrobium species utilized in the traditional Chinese medicine “Shihu”
Observing the microscopic characteristics of stems of the 24Dendrobium species, and their main identification features were summarized. The results showed that the identification ofDendrobium requires multiple microscopic characteristics. AbstractShihu is a famous Chinese Materia Medica derived from species of theDendrobium. In order to differentiate 24Dendrobium species, microscopic features of the 24Dendrobium species were systemically observed, including the trend of vascular bundle number with different internodes and the transverse section characteristics of stem. The results indicate that the thickness of cuticle, th...
Source: Microscopy Research and Technique - November 8, 2018 Category: Laboratory Medicine Authors: Yujiao Zhao,
Liangping Zha,
Bangxing Han,
Huasheng Peng Tags: RESEARCH ARTICLE Source Type: research
Real time monitoring and quantification of uptake carbon nanodots in eukaryotic cells
• Carbon nanodots have linear dependence between their concentration and emission intensity in aqueous solution and cytoplasm of living cells.• The relative emission intensity and concentration values of carbon nanodots were quantified by confocal microscope in Tobacco BY‐2 plant cells. AbstractThe real time monitoring and quantification of the concentration of highly fluorescent nitrogen ‐doped carbon nanodots (C‐dots) in eukaryotic Tobacco bright yellow‐2 (BY‐2) plant cells was investigated by fluorescence and confocal microscopy. The quantitative measurement of their fluorescent emission intensity was poss...
Source: Microscopy Research and Technique - November 8, 2018 Category: Laboratory Medicine Authors: Alexandre Loukanov,
Polina Mladenova,
Svetlin Toshev,
Asen Karailiev,
Elena Ustinovich,
Seiichiro Nakabayashi Tags: RESEARCH ARTICLE Source Type: research
Morpho ‐palynological assessment of medicinal flora of district Lahore, Pakistan based on LM and SEM
Microscopy Research and Technique, EarlyView. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - November 5, 2018 Category: Laboratory Medicine Authors: Shabnum Shaheen,
Mehwish Jaffer,
Farah Khan,
Khadim Hussain,
Uzma Hanif,
Sidra Younis,
Sobia Ilyas,
Saiqa Ishtiaq Source Type: research
