Evodiamine potentiates cisplatin-induced cell death and overcomes cisplatin resistance in non-small-cell lung cancer by targeting SOX9-beta-catenin axis
CONCLUSION: The combination of evodiamine and cisplatin may be a novel strategy for combating cisplatin resistance in NSCLC.PMID:38630183 | DOI:10.1007/s11033-024-09477-7 (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Munmun Panda Stuti Biswal Bijesh K Biswal Source Type: research
Mechanism of benzoxazinoids affecting the growth and development of Fusarium oxysporum f. sp. fabae
Plant Mol Biol. 2024 Apr 17;114(3):42. doi: 10.1007/s11103-024-01439-8.ABSTRACTContinuous cropping of faba bean (Vicia faba L.) has led to a high incidence of wilt disease. The implementation of an intercropping system involving wheat and faba bean can effectively control the propagation of faba bean wilt disease. To investigate the mechanisms of wheat in mitigating faba bean wilt disease in a wheat-faba bean intercropping system. A comprehensive investigation was conducted to assess the temporal variations in Fusarium oxysporum f. sp. fabae (FOF) on the chemotaxis of benzoxazinoids (BXs) and wheat root through indoor cult...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Zixuan Cen Bijie Hu Siyin Yang Guanglei Ma Yiran Zheng Yan Dong Source Type: research
Overview: Research on the Genetic Architecture of the Developing Cerebral Cortex in Norms and Diseases
Methods Mol Biol. 2024;2794:1-12. doi: 10.1007/978-1-0716-3810-1_1.ABSTRACTThe human brain is characterized by high cell numbers, diverse cell types with diverse functions, and intricate connectivity with an exceedingly broad surface of the cortex. Human-specific brain development was accomplished by a long timeline for maturation from the prenatal period to the third decade of life. The long timeline makes complicated architecture and circuits of human cerebral cortex possible, and it makes human brain vulnerable to intrinsic and extrinsic insults resulting in the development of variety of neuropsychiatric disorders. Unra...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Mariko Y Momoi Source Type: research
Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules
Methods Mol Biol. 2024;2794:79-94. doi: 10.1007/978-1-0716-3810-1_8.ABSTRACTReconstitution of intracellular transport in cell-free in vitro assays enables the understanding and dissection of the molecular mechanisms that underlie membrane traffic. Using total internal reflection fluorescence (TIRF) microscopy and microtubules, which are immobilized to a functionalized glass surface, the kinetic properties of single kinesin molecules can be imaged and analyzed in the presence or absence of microtubule-associated proteins. Here, we describe methods for the in vitro reconstitution of the motility of the neuronal kinesin motor...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Yani Suber Elias T Spiliotis Source Type: research
Generation of iPSCs Using Sendai Virus Vectors
Methods Mol Biol. 2024;2794:121-140. doi: 10.1007/978-1-0716-3810-1_11.ABSTRACTInduced pluripotent stem cells (iPSCs) are in vitro-derived cells capable of giving rise to several different cell types. The generation of iPSCs holds great promise for regenerative medicine and drug discovery research because it allows mature cells to be reprogrammed into a state of pluripotency. These highly versatile cells can then be induced to produce a variety of cell lineages and tissues by activating specific regulatory genes that drive their differentiation along distinct lineages. The great potential of these cells was recognized by S...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Hitomi Aoki Source Type: research
Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders
Methods Mol Biol. 2024;2794:141-155. doi: 10.1007/978-1-0716-3810-1_12.ABSTRACTHuman-induced pluripotent stem cell (hiPSC) technology has enabled comprehensive human cell-based disease modeling in vitro. Due to limited accessibility of primary human neurons as well as species-specific divergence between human and rodent brain tissues, hiPSC-derived neurons have become a popular tool for studying neuronal biology in a dish. Here, we provide methods for transcription factor-driven directed differentiation of neurons from hiPSCs via a neural progenitor cell (NPC) intermediate. Doxycycline-inducible expression of neuron fate-d...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Cheng Wang Jonas Cerneckis Yanhong Shi Source Type: research
Primary Culture of Dissociated Neurons from the Embryonic Cerebral Cortex
Methods Mol Biol. 2024;2794:169-175. doi: 10.1007/978-1-0716-3810-1_14.ABSTRACTPrimary neuronal culture is a valuable in vitro model for analyzing the molecular mechanisms underlying the development and function of neural circuits. In contrast to neurons in vivo, primary cultured neurons can easily be transfected with genes of interest or treated with chemicals such as agonists and inhibitors of a specific target molecule. Furthermore, time-dependent morphological changes, such as the acquisition of neuronal polarity, axon elongation, and dendrite branch formation, can be analyzed by using primary neuronal cultures. Here, ...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Shiho Ito Megumi Kawasaki Takeshi Kawauchi Source Type: research
In Vivo Whole-Cell Recording from the Mouse Brain
Methods Mol Biol. 2024;2794:245-257. doi: 10.1007/978-1-0716-3810-1_20.ABSTRACTMeasuring the membrane potential dynamics of neurons offers a comprehensive understanding of the molecular and cellular mechanisms that form their spiking activity, thus playing a crucial role in unraveling the mechanistic processes governing brain function. Techniques for intracellular recordings of membrane potentials pioneered in the 1940s have witnessed significant advancements since their inception. Among these, whole-cell patch-clamp recording has emerged as a leading method for measuring neuronal membrane potentials due to its high stabil...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Masahiro Kawatani Takayuki Yamashita Source Type: research
Genomic Copy Number Analysis Using Droplet Digital PCR: A Simple Method with EvaGreen Single-Color Fluorescent Design
Methods Mol Biol. 2024;2794:293-304. doi: 10.1007/978-1-0716-3810-1_24.ABSTRACTDroplet digital PCR (ddPCR) is an emerging method for the absolute quantification of PCR products, and it can detect DNA copy numbers accurately. It analyzes the end-point absolute fluorescence signals of the PCR-positive droplets and calculates the target concentration. EvaGreen is a nonspecific double-stranded DNA-binding fluorescent dye, and the ddPCR system also supports assays using this cost-effective hydrolysis probe. Here, we describe a simple method of quantification for DNA copy numbers using the EvaGreen single-color fluorescent desig...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Takeaki Tamura Taichi Imaizumi Keiko Shimojima Yamamoto Toshiyuki Yamamoto Source Type: research
Preparation of Healthy Single Neuron or Astrocyte Suspension from Adult Mouse Brain for RNA-seq
Methods Mol Biol. 2024;2794:341-351. doi: 10.1007/978-1-0716-3810-1_29.ABSTRACTSingle-cell RNA sequencing (scRNA-seq) has been widely applied in neuroscience research, enabling the investigation of cellular heterogeneity at the transcriptional level, the characterization of rare cell types, and the detailed analysis of the stochastic nature of gene expression. Isolation of single nerve cells in good health, especially from the adult rodent brain, is the most difficult and critical process for scRNA-seq. Here, we describe methods to optimize protease digestion of brain slices, which enable yield of millions of cells in good...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Neng-Yuan Hu Tian-Ming Gao Yi-Hua Chen Source Type: research
RIPK3 and RIPK1 gene expression in pterygium: unveiling molecular insights into pathogenesis
CONCLUSIONS: These findings suggest RIPK3 may play a protective role against pterygium recurrence through necroptosis.PMID:38630344 | DOI:10.1007/s11033-024-09368-x (Source: Mol Biol Cell)
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Mahnaz Divandari Amin Javadifar Arezoo Baradaran Moghadam Ali Akbar Janatabadi Source Type: research
A Methodology for Specific Disruption of Microtubule Polymerization into Dendritic Spines
Mol Biol Cell. 2024 Apr 17:mbcE24020093. doi: 10.1091/mbc.E24-02-0093. Online ahead of print.ABSTRACTDendritic spines, the mushroom-shaped extensions along dendritic shafts of excitatory neurons, are critical for synaptic function and are one of the first neuronal structures disrupted in neurodevelopmental and neurodegenerative diseases. Microtubule (MT) polymerization into dendritic spines is an activity-dependent process capable of affecting spine shape and function. Studies have shown that MT polymerization into spines occurs specifically in spines undergoing plastic changes. However, discerning the function of MT invas...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Elizabeth D Holland Hannah L Miller Matthew M Millette Russell J Taylor Gabrielle L Drucker Erik W Dent Source Type: research
STMND1 is A Phylogenetically Ancient Stathmin which Localizes to Motile Cilia and Exhibits Nuclear Translocation that Is Inhibited When Soluble Tubulin Concentration Increases
Mol Biol Cell. 2024 Apr 17:mbcE23120514. doi: 10.1091/mbc.E23-12-0514. Online ahead of print.ABSTRACTStathmins are small, unstructured proteins that bind tubulin dimers and are implicated in several human diseases, but whose function remains unknown. We characterized a new stathmin, STMND1 (Stathmin Domain Containing 1) as the human representative of an ancient sub-family. STMND1 features a N-terminal myristoylated and palmitoylated motif which directs it to membranes and a tubulin-binding stathmin-like domain (SLD) that contains an internal nuclear localization signal. Biochemistry and proximity labeling showed that STMND...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Xiang Deng Bryan O Seguinot Gary Bradshaw Jong Suk Lee Shannon Coy Marian Kalocsay Sandro Santagata Timothy Mitchison Source Type: research
Impact of two < em > Erwinia < /em > sp. on the response of diverse < em > Pisum sativum < /em > genotypes under salt stress
Physiol Mol Biol Plants. 2024 Feb;30(2):249-267. doi: 10.1007/s12298-024-01419-8. Epub 2024 Feb 25.ABSTRACTCurrently, salinization is impacting more than 50% of arable land, posing a significant challenge to agriculture globally. Salt causes osmotic and ionic stress, determining cell dehydration, ion homeostasis, and metabolic process alteration, thus negatively influencing plant development. A promising sustainable approach to improve plant tolerance to salinity is the use of plant growth-promoting bacteria (PGPB). This work aimed to characterize two bacterial strains, that have been isolated from pea root nodules, initia...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Houda Ilahi Elisa Zampieri Cristiana Sbrana Francesca Brescia Luca Giovannini Roghayyeh Mahmoudi Gholamreza Gohari Mustapha Missbah El Idrissi Mohamed Najib Alfeddy Martino Schillaci Lahcen Ouahmane Alice Calvo Fabiano Sillo Vasileios Fotopoulos Raffaella Source Type: research
Regulation of chloroplast biogenesis, development, and signaling by endogenous and exogenous cues
Physiol Mol Biol Plants. 2024 Feb;30(2):167-183. doi: 10.1007/s12298-024-01427-8. Epub 2024 Mar 11.ABSTRACTChloroplasts are one of the defining features in most plants, primarily known for their unique property to carry out photosynthesis. Besides this, chloroplasts are also associated with hormone and metabolite productions. For this, biogenesis and development of chloroplast are required to be synchronized with the seedling growth to corroborate the maximum rate of photosynthesis following the emergence of seedlings. Chloroplast biogenesis and development are dependent on the signaling to and from the chloroplast, which ...
Source: Mol Biol Cell - April 16, 2024 Category: Molecular Biology Authors: Kalyan Mahapatra Arpan Mukherjee Shikha Suyal Mansoor Ali Dar Lavanya Bhagavatula Sourav Datta Source Type: research
