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A novel porcine gene, MAPKAPK3, is differentially expressed in the pituitary gland from mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The mRNA differential display technique was performed to investigate the differences of gene expression in the pituitary gland from mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs. One novel gene differentially expressed was identified through semi-quantitative RT-PCR and its cDNA complete sequence was then obtained using the rapid amplification of cDNA ends (RACE) method. Nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence analysis revealed that the open reading frame of this gene encodes a protein of 384 amino acids has high homology with the mitoge...
Source: Molecular Biology Reports - November 18, 2009 Category: Molecular Biology Authors: Yonggang L Tags: Mol Biol Rep Source Type: journals

Dynamic regulation of glutamate decarboxylase 67 gene expression by alternative promoters and splicing during rat testis maturation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Glutamate decarboxylase produces GABA, the main inhibitory neurotransmitter in adult mammalian brain. Two homologous forms of GAD encoded by separate genes have been identified in mammalian brain, with molecular weight of 67 kDa (GAD67) and 65 kDa (GAD65). Here, we studied the transcriptional regulation of GAD67. Three transcript variants (GAD67A, GAD67B, and GAD67C) transcribed from distinct categories of transcriptional start sites were identified. RT-PCR revealed these transcripts have distinct tissues distributions. Though GAD67A and GAD67B were co-expressed in brain and many nonneural tissues, in heart, only GAD67...
Source: Molecular Biology Reports - November 13, 2009 Category: Molecular Biology Authors: Liu H, Zhang Y, Li S, Yan Y, Li Y Tags: Mol Biol Rep Source Type: journals

Identification and expression of an elastase homologue in Branchiostoma belcheri with implications to the origin of vertebrate pancreas.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we demonstrate the presence of an elastase homologue, named BbElas, in Branchiostoma belcheri. The recombinant BbElas hydrolyses the elastase specific substrate N-succinyl-Ala-Ala-Ala p-nitroanilide, which can be inhibited by the serine proteinase inhibitor PMSF, the elastase-specific inhibitor elastatinal and the cysteine proteinase inhibitor PCMB. Phylogenetic analysis shows that BbElas represents the archetype of vertebrate elastases, hinting at the clues that the different isoforms of vertebrate elastases are originated from an ancestral gene like BbElas. Our results also suggest that the mid-gut in amphi...
Source: Molecular Biology Reports - November 12, 2009 Category: Molecular Biology Authors: Sun T, Zhang S, Ji G Tags: Mol Biol Rep Source Type: journals

Inhibition of MAPK signaling by eNOS gene transfer improves ventricular remodeling after myocardial infarction through reduction of inflammation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) may play an important role in attenuating cardiac remodeling and apoptosis after myocardial infarction. However, the anti-inflammation effects of eNOS in infarcted myocardium and the role of MAPK signaling in eNOS/NO mediated cardiac remodeling have not yet been elucidated. Adenovirus carrying Human eNOS gene was delivered locally into heart 4 days prior to induction of myocardial infarction (MI) by left anterior descending coronary artery ligation. Monocyte/macrophage infiltration was detected by ED-1 immunohistochemistry. Western blot was employed to exam...
Source: Molecular Biology Reports - November 12, 2009 Category: Molecular Biology Authors: Chen LL, Zhu TB, Yin H, Huang J, Wang LS, Cao KJ, Yang ZJ Tags: Mol Biol Rep Source Type: journals

N-acetyltransferase 1 and 2 gene sequence variants and risk of head and neck cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study the role of genetically determined individual NAT1 and NAT2 genotypes, haplotypes and haplotype combinations in the predisposition to head and neck cancer was investigated. Polymorphic regions of the NAT1 and NAT2 genes were analyzed in patients with head and neck cancer and healthy individuals by polymerase chain reaction-restriction fragment length polymorphism. Distribution of the genotypes, allele frequencies, diplotypes and haplotypes and correlation with clinical characteristics were evaluated. No association was observed between the NAT1*3, NAT1*10, NAT1*11, NAT2*5 and NAT2*6 genotypes and risk of head...
Source: Molecular Biology Reports - November 8, 2009 Category: Molecular Biology Authors: Demokan S, Suoglu Y, Gözeler M, Demir D, Dalay N Tags: Mol Biol Rep Source Type: journals

NYGGF4 homologous gene expression in 3T3-L1 adipocytes: regulation by FFA and adipokines.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
NYGGF4 is a novel gene that is abundantly expressed in the adipose tissue of obese subjects and is involved in insulin resistance. In the present study, the mRNA expression of NYGGF4 homologous genes was examined in the 3T3-L1 cell line. The NYGGF4 mRNAs were expressed at low levels in the 3T3-L1 preadipocytes. During the conversion of 3T3-L1 preadipocytes to adipocytes, the expression of NYGGF4 mRNA was upregulated. On the 8th day after induction of differentiation, the NYGGF4 mRNA levels peaked and remained high. Free fatty acids (FFA) and tumor necrosis factor-alpha (TNFalpha) could upregulate NYGGF4 mRNA expression...
Source: Molecular Biology Reports - November 6, 2009 Category: Molecular Biology Authors: Zhao YP, Zhang CM, Zhu C, Chen XH, Wang JL, Ji CB, Chi X, Hong Q, Peng YZ, Guo XR Tags: Mol Biol Rep Source Type: journals

Molecular characterization and expression analysis of lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP) from pearl oyster Pinctada fucata.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP) plays an important function in the innate immune response of invertebrates as a pattern recognition receptor (PRR). Herein, we described the isolation and characterization of pearl oyster Pinctada fucata LGBP (designated as poLGBP). The poLGBP cDNA was 2,075 bp long and consisted of a 5'-untranslated region (UTR) of 18 bp, a 3'-UTR of 299 bp with one cytokine RNA instability motifs (ATTTA), and an open reading frame (ORF) of 1,758 bp encoding a polypeptide of 585 amino acids with an estimated molecular mass of 65.1 kDa and a theoretical isoelectric point...
Source: Molecular Biology Reports - November 4, 2009 Category: Molecular Biology Authors: Zhang D, Ma J, Jiang J, Qiu L, Zhu C, Su T, Li Y, Wu K, Jiang S Tags: Mol Biol Rep Source Type: journals

Gene expression profiling during gland morphogenesis of a mutant and a glandless upland cotton.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To identify genes involved in pigment gland morphogenesis in cotton, gene expression was profiled using genechip (Affymetrix) during pigment gland morphogenesis in cotton variety Xiangmian-18, which has glandless seeds but glanded plants, and a glandless line, N5. The results showed that 303 genes were differentially expressed by a factor greater than two during gland morphogenesis; 59% (180) of these genes shared similarity with known genes in GenBank. These genes play roles in defense response, response to oxidative stress, peroxidase activity, and other metabolic pathways. KOBAS (KEGG Orthology-Based Annotation Syst...
Source: Molecular Biology Reports - November 4, 2009 Category: Molecular Biology Authors: Sun Q, Cai Y, Xie Y, Mo J, Yuan Y, Shi Y, Li S, Jiang H, Pan Z, Gao Y, Chen M, He X Tags: Mol Biol Rep Source Type: journals

Cloning and characterization of a functional flavanone-3ss-hydroxylase gene from Medicago truncatula.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
As a key enzyme in the biosynthesis of flavonols, anthocyanidins and proanthocyanidins, flavanone-3ss-hydroxylase (F3H) plays very important roles in plant stress response. A putative flavanone-3ss-hydroxylase gene from Medicago truncatula (MtF3H), a model legume species, was identified from a bio-data analysis platform. It was speculated to be induced by salt stress based on the outcomes of the analysis platform. The complementary DNA (cDNA) consists of 1499 bp with an open reading frame (ORF) of 1098 bp, which encodes a putative protein of 365 amino acids with a molecular weight of about 41.36 kDa and an isoelectric ...
Source: Molecular Biology Reports - November 4, 2009 Category: Molecular Biology Authors: Shen X, Martens S, Chen M, Li D, Dong J, Wang T Tags: Mol Biol Rep Source Type: journals

Overexpression of CsNMAPK in tobacco enhanced seed germination under salt and osmotic stresses.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this research, biological function of CsNMAPK, encoding a mitogen-activated protein kinase of cucumber, was investigated under salt and osmotic stresses. Northern blot analysis showed that the expression of CsNMAPK was induced by salt and osmotic stresses in the cucumber root. In order to determine whether CsNMAPK was involved in plant tolerance to salt and osmotic stresses, transgenic tobacco plants constitutively overexpressing CsNMAPK were generated. Northern and Western blot analysis showed that strong signals were detected in the RNA and protein samples extracted from transgenic lines, whereas no signal was det...
Source: Molecular Biology Reports - November 4, 2009 Category: Molecular Biology Authors: Xu H, Li K, Yang F, Shi Q, Wang X Tags: Mol Biol Rep Source Type: journals

Molecular cloning and localization of a novel cotton annexin gene expressed preferentially during fiber development.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, a novel cotton annexin gene (designated as GhFAnnx) was isolated from a fiber cDNA library of cotton (Gossypium hirsutum). The full-length cDNA of GhFAnnx comprises an open reading frame of 945 bp that encodes a 314-amino acid protein with a calculated molecular mass of 35.7 kDa and an isoelectric point of 6.49. Genomic GhFAnnx sequences from different cotton species, TM-1, Hai7124 and two diploid progenitor cottons, G. herbaceum (A-genome) and G. raimondii (D-genome) showed that at least two copies of the GhFAnnx gene, each with six exons and five introns in the coding region, were identified in the allotet...
Source: Molecular Biology Reports - November 3, 2009 Category: Molecular Biology Authors: Wang LK, Niu XW, Lv YH, Zhang TZ, Guo WZ Tags: Mol Biol Rep Source Type: journals

Temporal and spatial expression analysis of PRGL in Gerbera hybrida.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
GASA-like genes form multigene families in diverse plant species and encode the proteins with a unique cysteine-rich domain (GASA domain). In our previously work, we cloned a GASA-like gene PRGL (Proline-rich GASA-like) from gerbera. Here we report the expression profiles of PRGL and the subcellular localization of PRGL protein. Multiple sequence alignment of the GASA domains indicates that PRGL shows the highest homology to AtPRGL (73.3% of amino acid identity) from Arabidopsis. Phylogenic analysis based on the full amino acid sequences indicates that PRGL and AtPRGL belong to a novel subfamily of GASA proteins. North...
Source: Molecular Biology Reports - November 3, 2009 Category: Molecular Biology Authors: Peng J, Lai L, Wang X Tags: Mol Biol Rep Source Type: journals

Identification of Elymus (Triticeae, Poaceae) and its related genera genomes by RFLP analysis of PCR-amplified Adh genes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Elymus L. is the largest genus in Triticeae, containing about 150 species with four recognized genome donors (St, H, P, and W). Traditionally, the genome compound of this genus is identified based on cytological data. Recently, molecular phylogenetic analysis was used to investigate its genomic combination. Here we describe a restriction fragment length polymorphism (RFLP) assay based on digesting alcohol dehydrogenase (Adh) amplicons with two restriction enzyme combinations, EcoRI-HindIII and EcoRI-PstI, which easily can be used to distinguish Elymus and its closely related genera genomes. The method includes only fou...
Source: Molecular Biology Reports - November 3, 2009 Category: Molecular Biology Authors: Liu Q, Zhang N, Li L, Liu J Tags: Mol Biol Rep Source Type: journals

Acid-sensing ion channels 3: a potential therapeutic target for pain treatment in arthritis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Acid-sensing ion channels 3 (ASIC3) is the most sensitive to such a pH change, predominantly distributed in the sensory peripheral nervous system, and strongly correlated with pain. Recently, there is increasing evidence that ASIC3 may contribute to the pathogenesis of chronic inflammatory pain diseases due to it is predominantly expressed in dorsal root ganglia (DRG) neurons making it a good candidate for a pain sensor. Elevated expression of ASIC3 was found in DRG of rodents with inflamed hind paws. In addition, it has been shown that ASIC3 gene knock-out mice (ASIC3-/-) exhibited no enhanced hyperalgesia in inflamed...
Source: Molecular Biology Reports - November 3, 2009 Category: Molecular Biology Authors: Yuan FL, Chen FH, Lu WG, Li X Tags: Mol Biol Rep Source Type: journals

TGFBR1*6A/9A polymorphism and cancer risk: a meta-analysis of 13,662 cases and 14,147 controls.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Published data on the association between TGFBR1*6A/9A polymorphism and cancer risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. A total of 32 studies including 13,662 cases and 14,147 controls were involved in this meta-analysis. Overall, significantly elevated cancer risks were associated with TGFBR1*6A in all genetic models (for allelic effect: OR = 1.11; 95% CI = 1.03-1.21; for 6A/6A vs. 9A/9A: OR = 1.30; 95% CI = 1.01-1.69; for 9A/6A vs. 9A/9A: OR = 1.08; 95% CI = 1.01-1.15; for dominant model: OR = 1.08; 95% CI = 1.02-1.15; for recessive model: OR = 1.2...
Source: Molecular Biology Reports - November 1, 2009 Category: Molecular Biology Authors: Liao RY, Mao C, Qiu LX, Ding H, Chen Q, Pan HF Tags: Mol Biol Rep Source Type: journals

Prediction and validation of conservative microRNAs of Solanum tuberosum L.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Potato (Solanum tuberosum) is an important crop around the world, and accounts for a significant amount of the food consumed by humans. However, little information is available about potato miRNAs which play important regulatory roles in plant growth and development. In the present study, computational prediction of potential miRNAs from potato revealed 71 miRNAs belonging to 48 families. Amongst these 71 mRNAs, 65 were predicted for the first time. Most potato miRNA families have one to three members, and sequence analysis showed that the candidate pre-miRNA sequences varied from 48 to 224 bp in length. To verify the ...
Source: Molecular Biology Reports - October 31, 2009 Category: Molecular Biology Authors: Yang W, Liu X, Zhang J, Feng J, Li C, Chen J Tags: Mol Biol Rep Source Type: journals

Cloning and expression analysis of a pollen preferential rapid alkalinization factor gene, BoRALF1, from broccoli flowers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rapid alkalinization factors (RALFs) are recently reported active peptide hormones and are considered to play important roles in plant development. We previously identified a differentially expressed cDNA fragment between cabbage flower buds of sterility lines and its maintainer line, which showed significant homology to Arabidopsis RALFL9. The novel RALF cDNA (BoRALF1) was isolated from broccoli flower buds by EST assembly. The open reading frame (ORF) comprises 240 bp, encoding a small putative preprotein of 79 amino acids (molecular weight of 8.72 kDa and a pI of 7.8), which contains the mature polypeptide at its C ...
Source: Molecular Biology Reports - October 30, 2009 Category: Molecular Biology Authors: Zhang GY, Wu J, Wang XW Tags: Mol Biol Rep Source Type: journals

Leptin plays a catabolic role on articular cartilage.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Leptin has been shown to play a crucial role in the regulation of body weight. There is also evidence that this adipokine plays a key role in the process of osteoarthritis. However, the precise role of leptin on articular cartilage metabolism is not clear. We investigate the role of leptin on articular cartilage in vivo in this study. Recombinant rat leptin (100 mug) was injected into the knee joints of rats, 48 h later, messenger RNA (mRNA) expression and protein levels of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), matrix metalloproteinases 2 and 9 (MMP-2, MMP-9), cathepsin D, an...
Source: Molecular Biology Reports - October 30, 2009 Category: Molecular Biology Authors: Bao JP, Chen WP, Feng J, Hu PF, Shi ZL, Wu LD Tags: Mol Biol Rep Source Type: journals

In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rieske protein gene in the Pacific oyster Crassostrea gigas was obtained by in silico cloning for the first time, and its expression profiles and subcellular localization were determined, respectively. The full-length cDNA of Cgisp is 985 bp in length and contains a 5'- and 3'-untranslated regions of 35 and 161 bp, respectively, with an open reading frame of 786 bp encoding a protein of 262 amino acids. The predicted molecular weight of 30 kDa of Cgisp protein was verified by prokaryotic expression. Conserved Rieske [2Fe-2S] cluster binding sites and highly matched-pair tertiary structure with 3CWB_E (Gallus gallus) we...
Source: Molecular Biology Reports - October 30, 2009 Category: Molecular Biology Authors: He X, Zhang Y, Yu Z Tags: Mol Biol Rep Source Type: journals

Targeted antitumor effect induced by hTERT promoter mediated ODC antisense adenovirus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The expression of Ornithine decarboxylase (ODC) which is the first key enzyme of polyamine biosynthesis is increased in cancer cells. We had blocked the polyamine synthesis pathway using the adenoviral-mediated antisense ODC in some cancer cells such as prostate cancers and colorectal cancers. These researches demonstrated that ODC antisense expression could inhibit tumor cell growth. In order to reach the goal of applying the targeting gene therapy in clinical practice, we cloned the antisense ODC RNA which was driven by cancer specific promoter (hTERT promoter; telomerase reverse transcriptase promoter) into the aden...
Source: Molecular Biology Reports - October 30, 2009 Category: Molecular Biology Authors: Wang W, Jin B, Li W, Xu CX, Cui FA, Liu B, Yan YF, Liu XX, Wang XL Tags: Mol Biol Rep Source Type: journals

CK2 phosphorylates TNFAIP1 to affect its subcellular localization and interaction with PCNA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
TNFAIP1 is a protein which can be induced by tumor necrosis factoralpha (TNFalpha) and interleukin-6 (IL-6), it may play roles in DNA synthesis, DNA repair, cell apoptosis and human diseases. However, very little has been known about how TNFAIP1 acts in these physiological processes. In this paper, CK2beta was identified as a partner of TNFAIP1 by screening the HeLa cDNA library in yeast two-hybrid system with TNFAIP1 as a bait. Furthermore, it was demonstrated that CK2 could phosphorylate TNFAIP1 in vitro and in vivo, which facilitated the distribution of TNFAIP1 in nucleus and enhanced its interaction with PCNA. It i...
Source: Molecular Biology Reports - October 23, 2009 Category: Molecular Biology Authors: Yang L, Liu N, Hu X, Zhang W, Wang T, Li H, Zhang B, Xiang S, Zhou J, Zhang J Tags: Mol Biol Rep Source Type: journals

Aldose reductase is a potent regulator of TGF-beta1 induced expression of fibronectin in human mesangial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Glomerulosclerosis is considered to be the final pathway leading to the progressive loss of renal function in several kidney diseases, transforming growth factor beta1 (TGF-beta1) plays a critical role in glomerulosclerosis. However, the mechanisms of TGF-beta1 stimulating glomerulosclerosis remain poorly understood. Here we report that TGF-beta1-induced expression of fibronectin (FN) depends on the activity of aldose reductase (AR) in human mesangial cells (HMCs).The results show that TGF-beta1 increased the expression of FN, which attenuated by pharmacological inhibition of AR or knockdown of the enzyme by small inte...
Source: Molecular Biology Reports - October 21, 2009 Category: Molecular Biology Authors: Huang P, Zhang Y, Jiang T, Zeng W, Zhang N Tags: Mol Biol Rep Source Type: journals

Analysis of Toxoplasma gondii surface antigen 2 gene (SAG2). Relevance of genotype I in clinical toxoplasmosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study contributes to the scarce available information on T. gondii at South America, and reinforces an emerging concept suggesting that SAG2 type I, rather than II, parasites are a frequent cause of clinical toxoplasmosis in this continent. PMID: 19842061 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 19, 2009 Category: Molecular Biology Authors: Sabaj V, Galindo M, Silva D, Sandoval L, Rodríguez JC Tags: Mol Biol Rep Source Type: journals

Characterization of unique heavy chain fibroin filaments spun underwater by the caddisfly Stenopsyche marmorata (Trichoptera; Stenopsychidae).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The silks of both Lepidoptera and its sister order Trichoptera contain a homologue of heavy chain (H-fibroin), which is assumed to determine the physical properties of the fiber, such as elasticity and toughness. The long repetitive region of the H-fibroin caddisfly Stenopsyche marmorata shows a conspicuous hierarchical structure that is composed of huge units, which are mainly constructed from four large blocks (SA, SB, SC and SD) arranged in an orderly fashion. Each block contains short, distinct motifs such as SXSXSX(SX), GPXG(X)(1-3) or triplet GGX, which also occur in lepidopteran and spider filaments. The SA, SB ...
Source: Molecular Biology Reports - October 19, 2009 Category: Molecular Biology Authors: Wang Y, Sanai K, Wen H, Zhao T, Nakagaki M Tags: Mol Biol Rep Source Type: journals

Identification of HSP27 as a potential tumor marker for colorectal cancer by the two-dimensional polyacrylamide gel electrophoresis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to use comparative proteomics technology to find the differentially expressed proteins between human colorectal carcinoma and the corresponding normal tumor-adjacent colorectal tissues. We have used the highly sensitive two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) for the identification of proteins differentially expressed in tumoral and neighboring normal mucosa. We have detected differences in abundance of 42 proteins with statistical variance of the tumor versus normal spot volume ratio within t...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Liu W, Ma Y, Huang L, Peng J, Zhang P, Zhang H, Chen J, Qin H Tags: Mol Biol Rep Source Type: journals

An examination of aspartate decarboxylase and glutamate decarboxylase activity in mosquitoes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we expressed two putative mosquito glutamate decarboxylase-like enzymes of mosquitoes and critically analyzed their substrate specificity and biochemical properties. Our results provide clear biochemical evidence establishing that one of them is an aspartate decarboxylase and the other is a glutamate decarboxylase. The mosquito aspartate decarboxylase functions exclusively on the production of beta-alanine with no activity with glutamate. Likewise the mosquito glutamate decarboxylase is highly specific to glutamate with essentially no activity with aspartate. Although insect aspartate decarboxylase shares hi...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Richardson G, Ding H, Rocheleau T, Mayhew G, Reddy E, Han Q, Christensen BM, Li J Tags: Mol Biol Rep Source Type: journals

p53 Promotes proteasome-dependent degradation of oncogenic protein HBx by transcription of MDM2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we attempted to identify the underlying mechanism. We found that overexpression of p53 protein reduces the level of HBx protein and shortens its half-life, however, in MDM2 knock out cells, p53 has no effects on degradation of HBx, meanwhile, overexpression of MDM2 in absence of p53 can accelerate turnover of HBx protein. These indicate that p53-mediated HBx degradation is MDM2-dependent. MDM2 interacts with HBx in vitro and in vivo but does not promote its ubiquitination. In consistent with the results above, HCC tissue samples with wild-type p53 hardly detect HBx protein, whereas, HBx always accumulate in ...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Xian L, Zhao J, Wang J, Fang Z, Peng B, Wang W, Ji X, Yu L Tags: Mol Biol Rep Source Type: journals

Investigation of LDHA and COPB1 as candidate genes for muscle development in the MYOD1 region of pig chromosome 2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Porcine MYOD1 gene has been mapped to swine chromosome (SSC) 2p14-p17, which is involved in the regulation of the proliferation and differentiation of skeletal muscle cells. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1) genes, which map close to MYOD1, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. However, little is known about the porcine LDHA and COPB1 genes. In the present study, the full-length cDNA of these two genes were cloned. The mapping results demonstrated that porcine LDHA and COPB1 we...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Qiu H, Xu X, Fan B, Rothschild MF, Martin Y, Liu B Tags: Mol Biol Rep Source Type: journals

Tissue-specific distribution of uncoupling proteins in normal rats and rats with high-fat-diet-induced obesity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Uncoupling proteins (UCPs) are a proton transporter family located in the mitochondrial inner membrane. Thus far, five molecules (UCP1-UCP5) have been identified as members of the UCP family. Recently, UCPs have attracted considerable interest in research on energy metabolism and obesity. However, to date, no study has focused on a comprehensive and systematic evaluation of the tissue-specific distribution of UCPs in obese individuals. Our study presents evidence of differential tissue expression profiles of five isoforms of UCPs in normal and diet-induced obese (DIO) rats using real-time polymerase chain reaction (PCR...
Source: Molecular Biology Reports - October 15, 2009 Category: Molecular Biology Authors: Zhang CM, Gu Y, Qing DN, Zhu JG, Zhu C, Zhang M, Guo XR Tags: Mol Biol Rep Source Type: journals

Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, an MnSOD gene (TaMnSOD) from Tamarix androssowii, under the control of the CaMV35S promoter, was introduced into poplar (Populus davidiana x P. bolleana). The physiological parameters, including SOD activity, malondialdehyde (MDA) content, relative electrical conductivity (REC) and relative weight gain, of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that SOD activity was enhanced in transgenic plants, and the MDA content and REC were significantly decreased compared to WT plants when exposed to NaCl stress. In addition, the relative weight gains of the transgeni...
Source: Molecular Biology Reports - October 14, 2009 Category: Molecular Biology Authors: Wang YC, Qu GZ, Li HY, Wu YJ, Wang C, Liu GF, Yang CP Tags: Mol Biol Rep Source Type: journals

Cyanobacteria MT gene SmtA enhance zinc tolerance in Arabidopsis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we report a functional analysis of SmtA in Arabidopsis thaliana and its response to zinc stress. After high zinc stress, the transgenic plants over-expressing SmtA showed higher survival rate than the wild type. We also found that over-expression of SmtA in Arabidopsis increased the activities of SOD and POD, and enhanced the tolerance to zinc stress. Together, our results indicate that SmtA may play an important role in the response to zinc stress in Arabidopsis. PMID: 19830591 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 14, 2009 Category: Molecular Biology Authors: Xu J, Tian YS, Peng RH, Xiong AS, Zhu B, Hou XL, Yao QH Tags: Mol Biol Rep Source Type: journals

Curcumin modulates eukaryotic initiation factors in human lung adenocarcinoma epithelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this study was to exam the curcumin cytotoxic effect and modulation of two major rate-limiting translation initiation factors, including eIF2alpha and eIF4E protein expression levels in lung adenocarcinoma epithelial cell line A549. Cytotoxicity was measured by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and protein changes were determined by Western blot. A549 cells were treated with 0-240 muM curcumin for 4-96 h. The inhibitory effects of curcumin on cytotoxicity were dose- and time-dependent (P < 0.001). The 50% inhibitory curcumin concentrations (IC50s) at 24, 48, 72, an...
Source: Molecular Biology Reports - October 14, 2009 Category: Molecular Biology Authors: Chen L, Tian G, Shao C, Cobos E, Gao W Tags: Mol Biol Rep Source Type: journals

Molecular interaction of alpha-synuclein with tubulin influences on the polymerization of microtubule in vitro and structure of microtubule in cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Microtubule dynamics is essential for many vital cellular processes such as in intracellular transport, metabolism, and cell division. Evidences demonstrate that alpha-synuclein may associate with microtubular cytoskeleton and its major component, tubulin. In the present study, the molecular interaction between alpha-synuclein and tubulin was confirmed by GST pull-down assay and co-immunoprecipitation. The interacting regions within alpha-synuclein with tubulin were mapped at the residues 60-100 of alpha-synuclein that is critical for the binding activity with tubulin. Microtubule assembly assays and sedimentation test...
Source: Molecular Biology Reports - October 13, 2009 Category: Molecular Biology Authors: Zhou RM, Huang YX, Li XL, Chen C, Shi Q, Wang GR, Tian C, Wang ZY, Jing YY, Gao C, Dong XP Tags: Mol Biol Rep Source Type: journals

Stress inducible heat shock protein 70: a potent molecular and toxicological signature in arsenic exposed broiler chickens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This communication reports about heat shock protein response after arsenic exposure in broiler chickens in vivo and in vitro both. Splenocytes harvested in presence of sodium arsenite expressed Heat shock protein 70 (HSP 70) which could be identified on the basis of relative migration pattern and western blot analysis. Serum levels of HSP 70 in broiler chicken also increased after continuous feeding of sodium arsenite in drinking water. This particular observation may be attributed towards systematic inflammation, oxidative stress and hepatocellular injury. In vitro relative quantification of transcription level of HSP...
Source: Molecular Biology Reports - October 13, 2009 Category: Molecular Biology Authors: Das S, Pan D, Bera AK, Rana T, Bandyopadhyay S, De S, Das SK, Bhattacharya D, Bandyopadhyay SK Tags: Mol Biol Rep Source Type: journals

S-adenosylmethionine stimulates fatty acid metabolism-linked gene expression in porcine muscle satellite cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Evidence indicates that both S-adenosylmethionine (SAMe) metabolism and intramuscular fat are associated with insulin resistance and type II diabetes. However, it is still unknown whether SAMe have effects on intramuscular adipogenesis. The present study investigated the roles of SAMe in the adipogenic differentiation of porcine muscle satellite cells. Cells isolated from neonatal pig muscle were treated with different concentrations of SAMe (0, 0.5 and 1.0 mM) for 24 h, induced for a 9-day adipogenic differentiation and were finally stained by oil red O staining. The adipocyte determination and differentiation factor-...
Source: Molecular Biology Reports - October 13, 2009 Category: Molecular Biology Authors: Yue T, Fang Q, Yin J, Li D, Li W Tags: Mol Biol Rep Source Type: journals

Effect of ketamine on ERK expression in hippocampal neural cell and the ability of learning behavior in minor rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To study the effects of ketamine on ERK expression in hippocampal neural cell and the ability of learning behavior in minor rats. Seventy-two Sprague-Dawley rats of 21 days old were randomly divided into nine groups. The Y-maze was used to test the ability of learning and spatial localization. At the end of training, all rats were killed and the expression levels of ERK1, ERK2 and p-ERK1/2 were tested by immunohistochemistry. The learning times and total reaction time (TRT) of group K2a, K2b, K2c and K3 have significant differences compared with T group (P < 0.05). Immunohistochemical staining showed that the level ...
Source: Molecular Biology Reports - October 13, 2009 Category: Molecular Biology Authors: Peng S, Zhang Y, Zhang J, Wang H, Ren B Tags: Mol Biol Rep Source Type: journals

SDF1-3' G801A polymorphisms in Polish patients with systemic lupus erythematosus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It has been reported that stromal cell-derived factor-1 (SDF1), currently also designated CXCL12, plays a significant role in the development of nephritis and death in the lupus mice model. Using restriction length fragment polymorphism (RFLP) analysis we assessed the frequencies of SDF1-3' G801A (rs 1801157) polymorphic variants between systemic lupus erythematosus (SLE) patients (n = 150) and controls (n = 300). There were no significant differences in the prevalence of SDF1-3' G801A polymorphic variants in SLE patients and healthy individuals. However, we observed that the SDF1-3' A/A and G/A genotypes (recessive mo...
Source: Molecular Biology Reports - October 13, 2009 Category: Molecular Biology Authors: Warchoł T, Lianeri M, Lącki JK, Jagodziński PP Tags: Mol Biol Rep Source Type: journals

Overexpression of PgDREB2A transcription factor enhances abiotic stress tolerance and activates downstream stress-responsive genes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we show that PgDREB2A from Pennisetum glaucum is a powerful transcription factor to engineer multiple stress tolerance in tobacco plants. The PgDREB2A protein lacks any potential PEST sequence, which is known to act as a signal peptide for protein degradation. Therefore, the transgenic tobacco plants were raised using full-length cDNA without modification. The transgenics exhibited enhanced tolerance to both hyperionic and hyperosmotic stresses. At lower concentration of NaCl and mannitol, seed germination and seedling growth was similar in WT and transgenic, however at higher concentration germination in WT ...
Source: Molecular Biology Reports - October 13, 2009 Category: Molecular Biology Authors: Agarwal P, Agarwal PK, Joshi AJ, Sopory SK, Reddy MK Tags: Mol Biol Rep Source Type: journals

Characterization of the human IRF-3 promoter and its regulation by the transcription factor E2F1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Interferon regulatory factor 3 (IRF-3), an essential transcriptional regulator of the interferon genes, has been implicated in virus and double-stranded RNA mediated induction of IFN-alpha, IFN-beta and RANTES, in virus-induced apoptosis and in tumor suppression. Promoter plays an important role in the regulation of gene expression, but the characterization of the human IRF-3 promoter has not been systematically analyzed in HEK 293 cells. To characterize the human IRF-3 promoter, we have isolated a genomic clone of the human IRF-3 gene promoter region containing 1,000 nucleotides of the 5'- flanking region. Transient t...
Source: Molecular Biology Reports - October 13, 2009 Category: Molecular Biology Authors: Xu HG, Ren W, Lu C, Zhou GP Tags: Mol Biol Rep Source Type: journals

A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the results of real time RT-PCR showed that the transcripts of DCA1 were induced by gradient concentration of sodium chloride. Subsequently, a structurally novel promoter containing highly repeated GT/AC sequences of the DCA1 gene was isolated, which was able to drive a stable expression of the foreign bar gene in transformed cells of D. salina, and the gradient concentrations of sodium chloride in media paralleled regulations in the levels of both proteins and mRNA of the bar gene driven by the DCA1 promoter. Furthermore, analysis of GUS activities revealed that the salt-inducible expression of the external...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Li J, Lu Y, Xue L, Xie H Tags: Mol Biol Rep Source Type: journals

Computational identification and characteristics of novel microRNAs from the silkworm (Bombyx mori L.).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
MicroRNAs (miRNAs) are a class of non-protein coding small RNAs that regulate expression of genes at post-transcriptional levels. Increasing evidence has shown that miRNAs play multiple roles in biological processes, including development, cell proliferation and apoptosis. Based on the conservation of miRNAs sequence, using a computational homology search based on genomic survey sequence analysis, a total of 16 novel miRNAs were identified and characteristics such as family and evolutionary conservation have been described. By using these newly identified miRNAs, the mRNA database of silkworm was blasted and 21 potenti...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Huang Y, Zou Q, Tang SM, Wang LG, Shen XJ Tags: Mol Biol Rep Source Type: journals

PriA participates in nascent DNA synthesis in Escherichia coli.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the participation of priA, B, C and rep genes in discontinuous DNA replication was examined by analyzing the size distribution of nascent DNA synthesized in wild-type, lig-7 and polA4113 genetic backgrounds. Inactivation of priA, but not priB, priC or rep, resulted in a significant increase of high molecular weight (HMW) DNA in the short pulse-labeled DNA in the wild-type lig ( + ) polA ( + ) strains. Inactivation of priA also produced a significant increase of HMW DNA in the nascent DNA synthesized in lig-7 and polA4113 strains. These results indicate that PriA is involved in the discontinuous synthesis of ...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Chen YY, Huang H, Wang TC Tags: Mol Biol Rep Source Type: journals

The interaction of lysozyme with caffeine, theophylline and theobromine in solution.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The interactions of lysozyme with caffeine (Caf), theophylline (Tph) and theobromine (Tbr) were investigated using UV-Vis absorption, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques. The results revealed that Caf (Tph or Tbr) caused the fluorescence quenching of lysozyme by the formation of Caf (Tph or Tbr)-lysozyme complex. The binding constants (K (A)) and thermodynamic parameters (DeltaG degrees , DeltaH degrees , DeltaS degrees ) at two different temperatures, the binding locality, and the binding power were obtained. The results showed that the process of binding Caf (...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Zhang HM, Tang BP, Wang YQ Tags: Mol Biol Rep Source Type: journals

Optimized procedure for expression and renaturation of recombinant human bone morphogenetic protein-2 at high protein concentrations.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A prokaryotic expression system has been used to produce recombinant human bone morphogenetic protein-2 (rhBMP-2). However, low rhBMP-2 yields and protein loss during purification and renaturation are the hurdles in the clinical application. Previous studies have indicated that variables such as temperature, host cell, salt concentration, and culture time affect the final rhBMP-2 yield. The optimization of these conditions in an Escherichia coli culture yielded 28.258 mg of rhBMP-2 per liter of culture. To reduce rhBMP-2 loss during purification and renaturation, we performed purification before renaturation in the pro...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Zhang H, Wu J, Zhang Y, Fu N, Wang J, Zhao S Tags: Mol Biol Rep Source Type: journals

Complete mitochondrial genome of Otis tarda (Gruiformes: Otididae) and phylogeny of Gruiformes inferred from mitochondrial DNA sequences.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The complete nucleotide sequence of mitochondrial genome of the Great bustard (Otis tarda) was determined by using polymerase chain reaction (PCR) method. The genome is 16,849 bp in size, containing 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. Sequences of the tRNA genes can be folded into canonical cloverleaf secondary structure except for tRNA-Cys and tRNA-Ser (AGY), which lose "DHU" arm. Sequence analysis showed that the O. tarda mitochondrial control region (mtCR) contained many elements in common with other avian mtCRs. A microsatellite repeat was found in the 3'-peripheral domain of the O. tarda mtCR...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Yang R, Wu X, Yan P, Su X, Yang B Tags: Mol Biol Rep Source Type: journals

The common variant in the GSTM1 and GSTT1 genes is related to markers of oxidative stress and inflammation in patients with coronary artery disease: a case-only study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recent studies suggest that the common variant in the GSTM1 and GSTT1 genes modifies the risk of coronary artery disease (CAD), however, it is unclear whether the risk of CAD modulated by variants in the GSTM1 and GSTT1 genes was associated with alterations of indices of oxidative stress and inflammation. Our study is an attempt to provide insight into the role of GST genetic variant and markers of oxidative stress and inflammation in CAD patients. A total of 719 Chinese CAD patients were successfully genotyped. Plasma total antioxidant status (TAOS), glutathione(GSH), C-reactive protein (CRP), fibrinogen (FIB) and whi...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Tang JJ, Wang MW, Jia EZ, Yan JJ, Wang QM, Zhu J, Yang ZJ, Lu X, Wang LS Tags: Mol Biol Rep Source Type: journals

Fas inhibition attenuates lipopolysaccharide-induced apoptosis and cytokine release of rat type II alveolar epithelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, inhibition of Fas can diminish LPS-induced apoptosis and inflammatory cytokine production in type II AECs, and Fas specific siRNAs may have therapeutic potentials for intervention of ALI/ARDS. PMID: 19823951 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Ma X, Xu D, Ai Y, Ming G, Zhao S Tags: Mol Biol Rep Source Type: journals

Population genetic analysis of 6 Y-STR loci in Chinese northwestern Qinchuan yellow cattle breed.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Six Y-STR loci (UMN0929, UMN0108, UMN0920, INRA124, UMN2404 and UMN0103) were analyzed using 576 healthy and unrelated males and 10 females of the Qinchuan cattle population in Chinese Shaanxi Province. Allele frequency, gene diversity, the polymorphic information content, and the number of effective gene were calculated. All loci were in accordance with the Hardy-Weinberg equilibrium (P > 0.05). The population data were compared with published data of other cattle breeds, suggesting that Qinchuan cattle were originated primarily from Bos Taurus. Results are valuable for individual identification, paternity testing,...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Xin Y, Zan L, Liu Y, Liu H, Tian W, Fan Y, Huang L Tags: Mol Biol Rep Source Type: journals

Recombinant human elafin protects airway epithelium integrity during inflammation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Elafin, an antiprotease, is likely to protect pulmonary epithelial cells from inflammatory damages. We aimed to explore the molecule mechanisms of recombinant human elafin on protecting A549 cells integrity against inflammatory assault. We transfected A549 airway epithelial cells with eukaryotic expression vector pEGFP-N1-Elafin and negative control vector pEGFP-N1, respectively. Cells were co-incubated with polymorphonuclear neutrophils (PMN) and then were stimulated with lipopolysaccharide (LPS). Results revealed that, in pEGFP-N1-Elafin transfected cells, neutrophil elastase (NE) activity significantly decreased aft...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Li Q, Zhou XD, Xu XY, Yang J Tags: Mol Biol Rep Source Type: journals

Molecular cloning, expression and subcellular distribution of an alternative splice variant of the porcine Sirt2 gene.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Sirt2, a NAD(+)-dependent histone deacetylase, plays a critical role in regulating lifespan, metabolism, mitosis and adipocyte differentiation. Here two bands of the porcine Sirt2 protein were found by western blotting, so we speculated existence of Sirt2 isoforms. Next, we cloned the porcine Sirt2 gene, and also found its alternative splice variant and named the novel splicing variant Sirt2(T). The complete cDNA sequence of Sirt2(T) is 1059 bp, encoding a deduced protein of 352 amino acids which is 39 amino acids shorter at the N-terminus than Sirt2. RT-PCR revealed that the Sirt2(T) mRNA is extensively expressed in p...
Source: Molecular Biology Reports - October 12, 2009 Category: Molecular Biology Authors: Liu B, Liu F, Bai L, Li Y, Yang G Tags: Mol Biol Rep Source Type: journals