Correction to: Reduced oxygen concentrations regulate the phenotype and function of human granulosa cells in vitro and cause a diminished steroidogenic but increased inflammatory cellular reaction
This is a correction to: Maria H öfner, Katja Eubler, Carola Herrmann, Ulrike Berg, Dieter Berg, Harald Welter, Axel Imhof, Ignasi Forné, Artur Mayerhofer, Reduced oxygen concentrations regulate the phenotype and function of human granulosa cellsin vitro and cause a diminished steroidogenic but increased inflammatory cellular reaction, Molecular Human Reproduction, Volume 30, Issue 1, January 2024, gaad049,https://doi.org/10.1093/molehr/gaad049 (Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - March 5, 2024 Category: Molecular Biology Source Type: research
Influx of innovation: promoting the scientific advances of early career investigators in Molecular Human Reproduction
Publication of data in peer-reviewed scholarly journals is the central engine that drives scientific discovery. It is the prime method by which results are disclosed and disseminated to other scientists and the public in a timely manner. Publishing results allows the scientific community to assess and validate findings and, most importantly, to use the data as a foundation to build future hypotheses and inform the next series of advances. (Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - February 29, 2024 Category: Molecular Biology Source Type: research
Differential role of bovine serum albumin and HCO3 − in the regulation of GSK3 alpha during mouse sperm capacitation
AbstractTo become fertile, mammalian sperm are required to undergo capacitation in the female tract orin vitro in defined media containing ions (e.g. HCO3 −, Ca2+, Na+, and Cl−), energy sources (e.g. glucose, pyruvate) and serum albumin (e.g. bovine serum albumin (BSA)). These different molecules initiate sequential and concomitant signaling pathways, leading to capacitation. Physiologically, capacitation induces changes in the sperm motility pattern (e.g. hyperactivation) and prepares sperm for the acrosomal reaction (AR), two events required for fertilization. Molecularly, HCO3 − activates the atypical adenyl...
Source: Molecular Human Reproduction - February 10, 2024 Category: Molecular Biology Source Type: research
Dysregulation of mucosal-associated invariant T cells correlates with altered placental microenvironment in preterm birth
AbstractPreterm birth (PTB) is a major problem affecting perinatal health, directly increasing the mortality risk of mother and infant that often results from the breakdown of the maternal –fetal immune balance. Increasing evidence shows the essential role of mucosal-associated invariant T (MAIT) cells to balance antibacterial function and immune tolerance function during pregnancy. However, the phenotype and function of placental MAIT cells and their specific mechanisms in PTB rema in unclear. Here, we report that MAIT cells in placentas from PTBs show increased activation levels and decreased IFN-γ secretion capacity ...
Source: Molecular Human Reproduction - February 3, 2024 Category: Molecular Biology Source Type: research
Racial disparity in uterine leiomyoma: new insights of genetic and environmental burden in myometrial cells
AbstractUterine leiomyoma (LM), also known as uterine fibroids, are common gynecological tumors and can reach a prevalence of 70% among women by the age of 50 years. Notably, the LM burden is much higher in Black women with earlier onset, a greater tumor number, size, and severity compared to White women. Published knowledge shows that there are genetic, environmental, and lifestyle-based risk factors associated with racial disparity for LM. Significant strides have been made on genomic, epigenomic, and transcriptomic data levels in Black and White women to elucidate the underlying pathomolecular reasons of racial dispar...
Source: Molecular Human Reproduction - January 30, 2024 Category: Molecular Biology Source Type: research
Comprehensive analysis of ovarian granulosa cell proteomics and phosphoproteomics in PCOS patients without insulin resistance
In this study, we describe the qu antitative protein expression profiles and phosphorylation-modified protein expression profiles of ovarian granulosa cells from patients with PCOS-NIR, providing a new research perspective for these patients. Further studies are required to elucidate the role of protein phosphorylation in PCOS. (Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - January 30, 2024 Category: Molecular Biology Source Type: research
HENMT1 is involved in the maintenance of normal female fertility in the mouse
AbstractPIWI-interacting small RNAs (piRNAs) maintain genome stability in animal germ cells, with a predominant role in silencing transposable elements. Mutations in the piRNA pathway in the mouse uniformly lead to failed spermatogenesis and male sterility. By contrast, mutant females are fertile. In keeping with this paradigm, we previously reported male sterility and female fertility associated with loss of the enzyme HENMT1, which is responsible for stabilising piRNAs through the catalysation of 3 ′-terminal 2′-O-methylation. However, theHenmt1 mutant females were poor breeders, suggesting they could be subfertile. ...
Source: Molecular Human Reproduction - September 30, 2021 Category: Molecular Biology Source Type: research
The human sperm basal body is a complex centrosome important for embryo preimplantation development
AbstractThe mechanism of conversion of the human sperm basal body to a centrosome after fertilization, and its role in supporting human early embryogenesis, has not been directly addressed so far. Using proteomics and immunofluorescence studies, we show here that the human zygote inherits a basal body enriched with centrosomal proteins from the sperm, establishing the first functional centrosome of the new organism. Injection of human sperm tails containing the basal body into human oocytes followed by parthenogenetic activation, showed that the centrosome contributes to the robustness of the early cell divisions, increasi...
Source: Molecular Human Reproduction - September 28, 2021 Category: Molecular Biology Source Type: research
NLRP3 promotes endometrial receptivity by inducing epithelial –mesenchymal transition of the endometrial epithelium
This study investigated the physiological and pathological significance of NLRP3 in human endometrial epithelial cell during the implantation window. NLRP3 is highly expressed during the mid-proliferative and mid-secretory phases of the human endometrium and transcriptionally up-regulated by estradiol (E2) through estrogen receptor β (ERβ). In addition, NLRP3 promotes embryo implantation and enhances epithelial-mesenchymal transition (EMT) of Ishikawa (IK) cells via both inflammasome-dependent and inflammasome-independent pathways, which might provide a novel insight into endometrial receptivity and embryo implantation. ...
Source: Molecular Human Reproduction - September 15, 2021 Category: Molecular Biology Source Type: research
Uterine kisspeptin receptor critically regulates epithelial estrogen receptor α transcriptional activity at the time of embryo implantation in a mouse model
This study investigated the role of the uterine kisspeptin receptor (KISS1R) in the molecular regulation of implantation in a mouse model. To conduct this study, a conditional uterine knockout (KO) ofKiss1r was created using thePgr-Cre (progesterone receptor-CRE recombinase) driver. Reproductive profiling revealed that while KO females exhibited normal ovarian function and mated successfully to stud males, they exhibited significantly fewer implantation sites, reduced litter size and increased neonatal mortality demonstrating that uterine KISS1R is required for embryo implantation and a healthy pregnancy. Strikingly, in th...
Source: Molecular Human Reproduction - September 15, 2021 Category: Molecular Biology Source Type: research
A pathogenic DMC1 frameshift mutation causes nonobstructive azoospermia but not primary ovarian insufficiency in humans
AbstractNonobstructive azoospermia (NOA) and diminished ovarian reserve (DOR) are two disorders that can lead to infertility in males and females. Genetic factors have been identified to contribute to NOA and DOR. However, the same genetic factor that can cause both NOA and DOR remains largely unknown. To explore the candidate pathogenic gene that causes both NOA and DOR, we conducted whole-exome sequencing (WES) in a non-consanguineous family with two daughters with DOR and a son with NOA. We detected one pathogenic frameshift variant (NM_007068:c.28delG, p. Glu10Asnfs*31) following a recessive inheritance mode in a meios...
Source: Molecular Human Reproduction - September 13, 2021 Category: Molecular Biology Source Type: research
Soluble adenylyl cyclase inhibition prevents human sperm functions essential for fertilization
AbstractSoluble adenylyl cyclase (sAC:ADCY10) has been genetically confirmed to be essential for male fertility in mice and humans. In mice,ex vivo studies of dormant, caudal epididymal sperm demonstrated that sAC is required for initiating capacitation and activating motility. We now use an improved sAC inhibitor, TDI-10229, for a comprehensive analysis of sAC function in mouse and human sperm. In contrast to caudal epididymal mouse sperm, human sperm are collected post-ejaculation, after sAC activity has already been stimulated. In addition to preventing the capacitation-induced stimulation of sAC and protein kinase A ac...
Source: Molecular Human Reproduction - August 31, 2021 Category: Molecular Biology Source Type: research
Differential expression of microRNAs in human endometrium after implantation of an intrauterine contraceptive device containing copper
In this study, the molecular mechanisms of abnormal uterine bleeding due to an intrauterine device containing copper were preliminarily investigated. The information will be beneficial for the clinical treatment of abnormal uterine bleeding caused by intrauterine device. (Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - August 24, 2021 Category: Molecular Biology Source Type: research
Leukaemia inhibitory factor modulates the differentiation of granulosa cells during sheep in vitro preantral to antral follicle development and improves oocyte meiotic competence
AbstractIn vitro follicle development from cryopreserved ovarian tissue could become an invaluable assisted reproduction technology for women with early ovarian failure. The challenge lies in producing, from small follicles present in the ovarian cortex, high-quality mature oocytes able to sustain embryo development.In vivo, an optimal combination of hormones and other factors coordinates the development of follicles and their enclosed oocyte. We have investigated the effect of the leukaemia inhibitory factor (LIF) cytokine, alone or in combination with FSH, on sheepin vitro follicle development from the preantral stage on...
Source: Molecular Human Reproduction - August 19, 2021 Category: Molecular Biology Source Type: research
Epigenetic regulation of reproduction in human and in animal models
epigeneticsimprintingchromatinhistonesassisted reproductive techniqueDNA methylationepigenetic marksepigenetic regulation of molecular reproduction (Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - July 28, 2021 Category: Molecular Biology Source Type: research
