Molecular MedicineThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

This page shows you the latest items in this publication.

Pages: 1 2 3 4 5 6

255 records returned

Plasma Cytokine Profiles in Preprotachykinin-A Knockout Mice Subjected to Polymicrobial Sepsis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
During the course of polymicrobial sepsis, a range of pro- and anti-inflammatory cytokines are produced by the host immune system. Successful recovery from sepsis involves striking a balance between these counteracting cytokines. We herein investigated the circulating cytokine profiles in preprotachykinin-A knockout (PPTA(-/-)) mice, which have been found to be significantly protected against microbial sepsis, by employing multiplexed bead-based suspension arrays for the measurement of 18 plasma cytokines. Four sets of PPTA(-/-) and wild-type mice, each with 6 mice, were subjected to cecal ligation and puncture-induced...
Source: Molecular Medicine - November 7, 2009 Category: Molecular Biology Authors: Hegde A, Uttamchandani M, Moochhala SM, Bhatia M Tags: Mol Med Source Type: journals

FOXP3 expression of micrometastasis-positive sentinel nodes in breast cancer patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A number of methods have been established for identifying sentinel nodes (SNs). In the present study, we attempted to clarify the immunological status of SNs with or without micrometastasis in breast cancer patients. SNs were identified by the dye- and gamma probe-guided method. Total RNA was extracted from the SNs, and the expression of T-BET, GATA-3, and FOXP3 were evaluated using quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Micrometastasis was identified as microscopically negative but positive by RT-PCR specific for mammaglobin. Of 88 patients, 17 (19.3%) showed positive metastas...
Source: Molecular Medicine - October 1, 2009 Category: Molecular Biology Authors: Matsuura K, Yamaguchi Y, Osaki A, Ohara M, Okita R, Emi A, Murakami S, Arihiro K Tags: Oncol Rep Source Type: journals

Human Ghrelin Ameliorate Organ Injury and Improves Survival after Radiation Injury Combined with Severe Sepsis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, human ghrelin is beneficial in a rat model of RCI. The protective effect of human ghrelin appears to be attributed to re-balancing the dysregulated sympathetic/parasympathetic nervous systems. PMID: 19779631 [PubMed - as supplied by publisher] (Source: Molecular Medicine)
Source: Molecular Medicine - September 24, 2009 Category: Molecular Biology Authors: Shah KG, Wu R, Jacob A, Blau SA, Ji Y, Dong W, Marini CP, Ravikumar TS, Cappa GF, Wang P Tags: Mol Med Source Type: journals

Prevalence and Significance of Coagulation Abnormalities in Community-acquired Pneumonia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, coagulation abnormalities were common and persistent in CAP, even among the least ill. These findings underscore the complexity of the coagulation response to infection and offer potential insights into coagulation-based therapeutics in clinical sepsis trials. PMID: 19753144 [PubMed - as supplied by publisher] (Source: Molecular Medicine)
Source: Molecular Medicine - September 14, 2009 Category: Molecular Biology Authors: Milbrandt EB, Reade MC, Lee M, Shook SL, Angus DC, Kong L, Carter M, Yealy DM, Kellum JA Tags: Mol Med Source Type: journals

Platelet factor 4 is highly upregulated in dendritic cells after severe trauma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dendritic cells (DC) represent an important linkage between the innate and adoptive immune system and express pro-inflammatory transcriptomic products early after trauma. Using a genomic approach, platelet factor 4 (PF4) was recently found significantly up-regulated in DCs following multiple trauma. However, knowledge about subsequent PF4 alteration and its potential clinical relevance in the context of multiple trauma is still limited.PF4 expression was analysed both in myeloid (MDC) and plasmocytoid (PDC) DCs isolated from 10 patients after multiple trauma by means of quantitative RT-PCR. Intracellular PF4 as well as...
Source: Molecular Medicine - September 9, 2009 Category: Molecular Biology Authors: Maier M, Geiger EV, Henrich D, Bendt C, Wutzler S, Lehnert M, Marzi I Tags: Mol Med Source Type: journals

Notice of retraction.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Authors: PMID: 19750127 [PubMed - in process] (Source: Molecular Medicine)
Source: Molecular Medicine - August 31, 2009 Category: Molecular Biology Tags: Mol Med Source Type: journals

Inhibition of proliferation, invasion, and migration of prostate cancer cells by down-regulating elongation factor-1 alpha expression.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSION: Taken together, these findings support the hypothesis that EF-1alpha affects multiple processes involved in tumor progression and identify EF-1alpha as a potential therapeutic target. PMID: 19707524 [PubMed - as supplied by publisher] (Source: Molecular Medicine)
Source: Molecular Medicine - August 23, 2009 Category: Molecular Biology Authors: Zhu G, Yan W, He HC, Bi XC, Han ZD, Dai QS, Ye YK, Liang YX, Wang J, Zhong W Tags: Mol Med Source Type: journals

Serum Abeta levels as predictors of conversion to MCI/AD in an ADAPT subcohort.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recent evidence suggest an association of beta-amyloid (Abeta) with vascular risk factors and the medications to treat them, which could potentially obfuscate Abeta's usefulness in predicting mild cognitive impairment (MCI) or Alzheimer's disease (AD). In a subcohort from the Alzheimer's disease Anti-inflammatory Prevention Trial (enriched for family history of AD), we investigated whether systolic blood pressure, total cholesterol, triglycerides, serum creatinine, APOE, statin and anti-hypertensive use influence the predictive value of serum Abeta for MCI/AD over two years. Blood samples were collected to quantify ser...
Source: Molecular Medicine - August 23, 2009 Category: Molecular Biology Authors: Abdullah L, Luis C, Paris D, Mouzon B, Ait-Ghezala G, Keegan AP, Wang D, Crawford F, Mullan M Tags: Mol Med Source Type: journals

European Population Genetic Substructure: Further Definition of Ancestry Informative Markers for Distinguishing Among Diverse European Ethnic Groups.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The definition of European population genetic substructure and its application to understanding complex phenotypes is becoming increasingly important. In the current study using over 4000 subjects genotyped for 300 thousand SNPs we provide further insight into relationships among European population groups and identify sets of SNP ancestry informative markers (AIMs) for application in genetic studies. In general, the graphical description of these principal components analyses (PCA) of diverse European subjects showed a strong correspondence to the geographical relationships of specific countries or regions of origin. ...
Source: Molecular Medicine - August 23, 2009 Category: Molecular Biology Authors: Tian C, Kosoy R, Nassir R, Lee A, Villoslada P, Klareskog L, Hammarström L, Garchon HJ, Pulver AE, Ransom M, Gregersen PK, Seldin MF Tags: Mol Med Source Type: journals

Novel Null-allele Mutations and Genotype-Phenotype Correlation in Argentinean Patients with Erythropoietic Protoporphyria: Erythropoietic Protoporphyria in Argentina.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Erythropoietic Protoporphyria (EPP) is an inherited disorder of porphyrin metabolism in which decreased activity of ferrochelatase (FECH) leads to accumulation of protoporphyrin IX (PP IX) in red blood cells, plasma, liver, bile and increased excretion in feces. Clinically, EPP is characterized by photosensitivity that includes burning, swelling, itching and painful erythema in sun exposed areas from early childhood. Chronic liver disease is an important complication in a minority of EPP patients and in some cases liver transplantation has been performed. So far, about 110 different mutations and several polymorphisms ...
Source: Molecular Medicine - August 19, 2009 Category: Molecular Biology Authors: Parera VE, Koole RH, Minderman G, Edixhoven A, Rossetti MV, Batlle A, DE Rooij FW Tags: Mol Med Source Type: journals

Persistent fetal gamma-globin expression in adult transgenic mice following deletion of two silencer elements located 3' to the human Agamma-globin gene.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Natural deletions of the human beta-globin gene cluster lead to specific syndromes characterized by increased production of fetal hemoglobin in adult life and provide a useful model to delineate novel cis-acting elements involved in the developmental control of hemoglobin switching. A hypothesis accounting for these phenotypic features, assumes that silencers located within the Agamma to delta-gene region, are deleted in hereditary persistence of fetal hemoglobin (HPFH) and deltabeta-thalassemias, leading to failure of switching. In the present study, we sought to clarify the in vivo role of two elements, termed Enh an...
Source: Molecular Medicine - August 16, 2009 Category: Molecular Biology Authors: Gazouli M, Katsantoni E, Kosteas T, Anagnou NP Tags: Mol Med Source Type: journals

Expression Pattern of Stemness-Related Genes in Human Endometrial and Endometriotic Tissues.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed at analysing in endometrial and endometriotic tissues the differential expression of a panel of genes involved in preservation of stemness status and consequently considered as markers of stem cell presence.The expression profiles of a panel of 13 genes (SOX2, SOX15, ERAS, SALL4, OCT4, NANOG, UTF1, DPPA2, BMI1, GDF3, ZFP42, KLF4, TCL1) were analysed by RT-PCR in human endometriotic (n=12) and endometrial samples (n=14). The expression of SALL4 and OCT4 was further analysed by immunohistochemistry.Genes UTF1, TCL1 and ZFP42 showed a trend for higher frequency of expression in endometriosis than in endometri...
Source: Molecular Medicine - August 16, 2009 Category: Molecular Biology Authors: Forte A, Schettino MT, Finicelli M, Cipollaro M, Colacurci N, Cobellis L, Galderisi U Tags: Mol Med Source Type: journals

Testase 1 (ADAM 24) a sperm surface metalloprotease is required for normal fertility in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ADAM family members play important roles in various physiological and pathological processes, for example, fertilization, embryogenesis, neurogenesis, and development of asthma and arthritis (Primakoff and Myles, 2000. Trends Genet 16: 83-87; Edwards et al., 2008. Mol Aspects Med 29: 258-289). We previously reported that testase 1 (ADAM 24) is the first identified metalloprotease present on the surface of mature sperm. To investigate a potential role of testase 1 in fertilization, we generated testase 1 deficient mice. Testase 1 null male mice showed reduced fertility, producing only half the number of offspring when c...
Source: Molecular Medicine - August 6, 2009 Category: Molecular Biology Authors: Zhu GZ, Gupta S, Myles DG, Primakoff P Tags: Mol Reprod Dev Source Type: journals

Rhizobium tibeticum sp. nov., a symbiotic bacterium isolated from Medicago archiducis-nicolai Vassilcz.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Isolated from root nodules of Medicago archiducis-nicolai Vassilcz grown in Tibet, China, the bacterial strains CCBAU 85039T and CCBAU 85027 were gram-negative, aerobic, motile, non-spore-forming rods, with colonies semi-translucent, opalescent on yeast-extract-mannitol agar. In numerical taxonomy, SDS-PAGE analysis of whole cell proteins and DNA-DNA hybridization, these two strains were very similar and were different from the reference strains for defined Rhizobium species. In the phylogeny of 16S rRNA gene, they were most similar to Rhizobium etli CFN 42T (98.2% similarity) and R. leguminosarum USDA 2370T (97.6% sim...
Source: Molecular Medicine - July 29, 2009 Category: Molecular Biology Authors: Hou BC, Wang ET, Li Y, Jia RZ, Chen WF, Gao Y, Dong R, Chen WX Tags: Int J Syst Evol Microbiol Source Type: journals

Progesterone with vitamin D affords better neuroprotection against excitotoxicity in cultured cortical neurons than progesterone alone.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, the findings of the present study can be taken to suggest that VDH warrants study as a potential partner for combination therapy with PROG. PMID: 19603099 [PubMed - as supplied by publisher] (Source: Molecular Medicine)
Source: Molecular Medicine - July 14, 2009 Category: Molecular Biology Authors: Atif F, Sayeed I, Ishrat T, Stein DG Tags: Mol Med Source Type: journals

CD151 gene delivery after myocardial infarction promotes functional neovascularization and activates FAK signaling.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study is to assess whether CD151 induces arteriogenesis and promotes functional neovascularization in a pig myocardial infarction model and to determine the signaling pathways involved. CD151 cDNA and antiCD151 sequence were constructed into a recombinant adeno-associated virus (rAAV) vector. All 26 pigs were subjected to either coronary artery ligation or no surgery. Eight weeks after viral administration, the expression of CD151 protein was measured by western blot. The densities of capillaries and arterioles were determined using immunohistochemistry. (13)N-labeled NH(3) positron emission computed tomography ((13)N...
Source: Molecular Medicine - July 14, 2009 Category: Molecular Biology Authors: Zuo H, Liu Z, Liu X, Yang J, Liu T, Wen S, Zhang X, Cianflone K, Wang D Tags: Mol Med Source Type: journals

Role of E2F1-cyclinE1-cyclinE2 circuit in human coronary smooth muscle cell proliferation and therapeutic potential of its down regulation by siRNAs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Aberrant coronary vascular smooth muscle cell (CSMC) proliferation represents a pivotal event underlying intimal hyperplasia, a phenomenon impairing the long term efficacy of bypass surgery and angioplasty procedures. Consequently research has become focused on efforts to identify molecules that are able to control CSMC proliferation. Here, the down regulation of CSMC growth was investigated by small interfering RNAs (siRNAs) targeted against E2F1, cyclin E1 and E2, genes whose contribution to CSMC proliferation is only now emerging.Chemically synthesized siRNAs were delivered by two different transfection reagents to ...
Source: Molecular Medicine - July 14, 2009 Category: Molecular Biology Authors: Dapas B, Farra R, Grassi M, Giansante C, Fiotti N, Uxa L, Rainaldi G, Mercatanti A, Colombatti A, Spessotto P, Lacovich V, Guarnieri G, Grassi G Tags: Mol Med Source Type: journals

Regulatory NK Cell Functions in Inflammation and Autoimmunity.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Natural killer cells were traditionally viewed as cytotoxic effector cells whose rapid killing of infected and transformed cells without preactivation provides a first line on defense prior to the initiation of an adaptive immune response against infection and tumor development. However, it has become clear that NK interact with various components of the immune system, and therefore have the potential to function as regulatory cells. While NK cells can assist in dendritic cell (DC) maturation and T cell polarization, increasing evidence indicates that NK cells can also limit adaptive immune responses and regulate autoi...
Source: Molecular Medicine - July 14, 2009 Category: Molecular Biology Authors: Lünemann A, Lünemann JD, Münz C Tags: Mol Med Source Type: journals

Severe Burn-Induced Endoplasmic Reticulum Stress and Hepatic Damage in Mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Severe burn injury results in liver dysfunction and damage, with subsequent metabolic derangements contributing to patient morbidity and mortality. On a cellular level, there is significant hepatocyte apoptosis post-burn, which likely contributes to liver dysfunction. However, the underlying mechanisms of hepatocyte apoptosis are poorly understood. The endoplasmic reticulum (ER) stress response/unfolded protein response (UPR) pathway can lead to hepatocyte apoptosis under conditions of liver dysfunction. Thus, we hypothesized that ER stress/UPR may mediate hepatic dysfunction in response to burn injury. Here, we invest...
Source: Molecular Medicine - July 14, 2009 Category: Molecular Biology Authors: Song J, Finnerty CC, Herndon DN, Boehning D, Jeschke MG Tags: Mol Med Source Type: journals

Osteopontin is associated with increased arterial stiffness in Rheumatoid Arthritis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rheumatoid arthritis (RA) patients are characterized by increased arterial stiffness, an independent predictor of cardiovascular risk. It has been suggested that osteopontin (OPN), a cytokine involved in RA pathogenesis, might have vascular effects. To study a possible relationship between OPN and arterial stiffness, aortic pulse-wave velocity (PWV) was measured by tonometry in 69 patients, 41 with RA, 28 with systemic sclerosis (SSc) and 18 healthy controls. Plasma OPN levels, oxidative stress markers and ET-1 were assessed.OPN levels were significantly (p<0.05) higher in RA (median 9.93; range 4.36-47.80 ng/ml) th...
Source: Molecular Medicine - July 14, 2009 Category: Molecular Biology Authors: Bazzichi L, Ghiadoni L, Rossi A, Bernardini M, Lanza M, De Feo F, Giacomelli C, Mencaroni I, Raimo K, Rossi M, Mazzone AM, Taddei S, Bombardieri S Tags: Mol Med Source Type: journals

The inverse association between cardiorespiratory fitness and C-reactive protein is mediated by autonomic function: a possible role of the cholinergic anti-inflammatory pathway.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although studies have shown an inverse association between cardiorespiratory fitness (CRF) and C-reactive protein (CRP) levels, the underlying mechanisms are not fully understood. There is emerging evidence that autonomic nervous system function is related to CRP levels. Because high CRF is related to improved autonomic function, we hypothesized that the association between high CRF and low CRP levels would be affected by autonomic nervous system function. Cross-sectional analyses were conducted on 2456 asymptomatic men who participated in a medical screening program. Fasting blood samples for cardiovascular disease ri...
Source: Molecular Medicine - July 14, 2009 Category: Molecular Biology Authors: Jae SY, Heffernan KS, Yoon ES, Lee MK, Fernhall B, Park WH Tags: Mol Med Source Type: journals

Intratracheal Delivery of CX3CL1-expressing Mesenchymal Stem Cells to Multiple Lung Tumors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: These results suggest that MSCs expressing anti-tumor agents can be delivered intratracheally into multiple lung tumor tissues without causing inflammation. PMID: 19603106 [PubMed - as supplied by publisher] (Source: Molecular Medicine)
Source: Molecular Medicine - July 14, 2009 Category: Molecular Biology Authors: Xin H, Sun R, Kanehira M, Takahata T, Itoh J, Mizuguchi H, Saijo Y Tags: Mol Med Source Type: journals

The selective alpha7 agonist GTS-21 attenuates cytokine production in human whole blood and human monocytes activated by ligands for TLR2, TLR3, TLR4, TLR9, and RAGE.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report here our findings that GTS-21 attenuates tumor necrosis factor (TNF) and interleukin 1beta levels in human whole blood activated by exposure to endotoxin. GTS-21 inhibited TNF production in endotoxin-stimulated primary human monocytes in vitro at the transcriptional level. The suppressive effect of GTS-21 was more potent than nicotine in whole blood and monocytes. Furthermore, GTS-21 attenuated TNF production in monocytes stimulated with peptidoglycan, polyinosinic-polycytidylic acid, CpG, HMGB1 (high-mobility group box 1 protein), and advanced glycation end product-modified albumin. GTS-21 decreased TNF levels i...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Rosas-Ballina M, Goldstein RS, Gallowitsch-Puerta M, Yang L, Valdés-Ferrer SI, Patel NB, Chavan S, Al-Abed Y, Yang H, Tracey KJ Tags: Mol Med Source Type: journals

Coordinate control of expression of Nrf2-modulated genes in the human small airway epithelium is highly responsive to cigarette smoking.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nuclear factor erythroid 2-related factor 2 (Nrf2) is an oxidant-responsive transcription factor known to induce detoxifying and antioxidant genes. Cigarette smoke, with its large oxidant content, is a major stress on the cells of small airway epithelium, which are vulnerable to oxidant damage. We assessed the role of cigarette smoke in activation of Nrf2 in the human small airway epithelium in vivo. Fiberoptic bronchoscopy was used to sample the small airway epithelium in healthy-nonsmoker and healthy-smoker, and gene expression was assessed using microarrays. Relative to nonsmokers, Nrf2 protein in the small airway e...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Hübner RH, Schwartz JD, De Bishnu P, Ferris B, Omberg L, Mezey JG, Hackett NR, Crystal RG Tags: Mol Med Source Type: journals

A genomic score prognostic of outcome in trauma patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Traumatic injuries frequently lead to infection, organ failure, and death. Health care providers rely on several injury scoring systems to quantify the extent of injury and to help predict clinical outcome. Physiological, anatomical, and clinical laboratory analytic scoring systems (Acute Physiology and Chronic Health Evaluation [APACHE], Injury Severity Score [ISS]) are utilized, with limited success, to predict outcome following injury. The recent development of techniques for measuring the expression level of all of a person's genes simultaneously may make it possible to develop an injury scoring system based on th...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Warren HS, Elson CM, Hayden DL, Schoenfeld DA, Cobb JP, Maier RV, Moldawer LL, Moore EE, Harbrecht BG, Pelak K, Cuschieri J, Herndon DN, Jeschke MG, Finnerty CC, Brownstein BH, Hennessy L, Mason PH, Tompkins RG, Tags: Mol Med Source Type: journals

Short-term overeating induces insulin resistance in fat cells in lean human subjects.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined insulin signaling in adipocytes from lean volunteers, before and at the end of a 4-wk period of consuming a fast-food, high-calorie diet that led to weight gain. We also examined adipocytes from patients with T2D. During the high-calorie diet, subjects gained 10% body weight and 19% total body fat, but stayed lean (body mass index = 24.3 kg/m(2)) and developed moderate systemic insulin resistance. Similarly to the situation in T2D subjects, in subjects on the high-calorie diet, the amount of insulin receptors was reduced and phosphorylation of IRS1 at tyrosine and at serine-307 (human sequence, corresponding to...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Danielsson A, Fagerholm S, Ost A, Franck N, Kjolhede P, Nystrom FH, Strålfors P Tags: Mol Med Source Type: journals

Nonerythropoietic tissue protective compounds are highly effective facilitators of wound healing.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Erythropoietin (EPO) is a type I cytokine that utilizes different receptor isoforms either to maintain hematopoiesis or protect against injuries that arise from widely diverse etiologies. EPO also facilitates healing by reducing inflammation and mobilizing endothelial progenitor cells to participate in restorative neoangiogenesis, but it is unclear which EPO receptor isoform is responsible for healing and whether this receptor use varies according to the type of wound. In the present studies carried out in the rat, we have utilized receptor-selective derivatives of EPO to determine which receptor type operates in (i) a...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Erbayraktar Z, Erbayraktar S, Yilmaz O, Cerami A, Coleman T, Brines M Tags: Mol Med Source Type: journals

The immunohistochemical expression of growth hormone-releasing hormone receptor splice variant 1 is a favorable prognostic marker in colorectal cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hypothalamic growth hormone (GH)-releasing hormone (GHRH) regulates the release of GH from the pituitary gland. The receptors for GHRH (GHRH-R) are expressed predominantly in the pituitary. Recent evidence demonstrates that splice variants of the GHRH receptor are also expressed in several nonpituitary tissues, both normal and tumoral, as well as in cancer cell lines. The aim of this study was to investigate the expression of the splice variant 1 (SV-1) of GHRH-R in colorectal cancer (CRC). Seventy patients who underwent partial colectomy for CRC were enrolled in the study. Immunohistochemical expression of SV-1 was st...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Theophanous E, Petraki C, Scorilas A, Komborozos V, Veloudis G, Varga JL, Zarandi M, Schally AV, Koutsilieris M Tags: Mol Med Source Type: journals

Cadmium concentrations in blood and seminal plasma: correlations with sperm number and motility in three male populations (infertility patients, artificial insemination donors, and unselected volunteers).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To investigate a possible common environmental exposure that may partially explain the observed decrease in human semen quality, we correlated seminal plasma and blood cadmium levels with sperm concentration and sperm motility. We studied three separate human populations: group 1, infertility patients (Long Island, NY, USA); group 2, artificial insemination donors (AID) (Rochester, NY, USA); and group 3, general population volunteers (Rochester, NY, USA). Information about confounding factors was collected by questionnaire. Seminal plasma cadmium did not correlate with blood cadmium (Spearman correlation, n = 91, r = -...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Benoff S, Hauser R, Marmar JL, Hurley IR, Napolitano B, Centola GM Tags: Mol Med Source Type: journals

Does splenectomy protect against immune-mediated complications in blunt trauma patients?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Activation of the innate immune system results from severe trauma and the resultant systemic inflammatory response is thought to mediate remote organ injury. In animal models, vagal-mediated innate immune responses have been shown to modulate proinflammatory cytokine release in response to trauma or sepsis. In those models, vagal nerve transaction and splenectomy decreased cytokine release and protected against lung injury and mortality. We hypothesized that, if similar mechanisms are active in humans, patients who require splenectomy for trauma would have better outcomes than injured patients without splenectomy. We p...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Crandall M, Shapiro MB, West MA Tags: Mol Med Source Type: journals

Genome-wide association for smoking cessation success: participants in a trial with adjunctive denicotinized cigarettes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The ability to quit smoking successfully displays substantial heritability in classical and molecular genetic studies. Twin studies suggest that some of the genetics for the ability to quit overlap with genetic components of nicotine dependence, but many do not. Genome-wide association (GWA) studies have demonstrated haplotypes that distinguish successful quitters from individuals who were not able to quit smoking in: i) clinical trials that employed nicotine replacement; ii) clinical trials that employed bupropion; and iii) community quitter samples. We now report novel GWA results from participants in a clinical tria...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Drgon T, Johnson C, Walther D, Albino AP, Rose JE, Uhl GR Tags: Mol Med Source Type: journals

Spermine protects mice against lethal sepsis partly by attenuating surrogate inflammatory markers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The pathogenesis of sepsis is partly attributable to dysregulated inflammatory response mediated by pathogen-associated molecular patterns (PAMPs) (for example, endotoxin) and damage-associated molecular patterns (DAMPs) (for example, high-mobility group box 1 [HMGB1]). An endogenous ubiquitous polyamine, spermine, inhibits endotoxin-induced cytokine release in vitro, but its capacities to attenuate sepsis- and HMGB1-induced inflammatory responses was previously unknown. We thus tested the hypothesis that spermine protects mice against lethal sepsis by attenuating sepsis-induced local and systemic inflammatory response...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Zhu S, Ashok M, Li J, Li W, Yang H, Wang P, Tracey KJ, Sama AE, Wang H Tags: Mol Med Source Type: journals

Molecularly targeted therapies for dysimmune neuropathies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conventional treatment options, including corticosteroids, intravenous immunoglobulin, or plasma exchange, often fail to treat dysimmune neuropathies, such as chronic inflammatory demyelinating polyneuropathy, multifocal motor neuropathy, and monoclonal gammopathy with its subtypes. Therefore, a significant percentage of patients require adjunctive immunosuppressive therapies. Considering that even immunosuppressive agents often are ineffective and/or associated with significant toxicities, the need for the development of safe and effective new treatment options is rising. Currently, several monoclonal antibodies (MAbs...
Source: Molecular Medicine - June 30, 2009 Category: Molecular Biology Authors: Argyriou AA Tags: Mol Med Source Type: journals

Stability of the hydration layer of tropocollagen: A QM study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Collagen is a triple helical protein, highly hydrated in nature. Bella and Berman (J Mol Biol 1996, 264, 734) have reported the structure of the first hydration layer. Water molecules form bridges of different length around the POG repeats and self assemble into left-handed helical water threads. To explore the stability of these specifically hydrated places, we have designed suitable QM models: each comprises a triple helix formed by 18 residues surrounded by 8 to 12 explicit waters. Two sets of amino acids were used, one standing for the core structural subunit of tropocollagen (POG-model) and one for its natural enz...
Source: Molecular Medicine - June 29, 2009 Category: Molecular Biology Authors: Pálfi VK, Perczel A Tags: J Comput Chem Source Type: journals

Anomaly in aortic arch alters pathological outcome of transient global ischemia in Rhesus macaques.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We investigated a non-human primate (NHP) transient global ischemia (TGI) model which was induced by clipping the arteries originating from the aortic arch. Previously we demonstrated that our TGI model in adult Rhesus macaques (Macaca mulatta) results in marked neuronal cell loss in the hippocampal region, specifically the cornu Ammonis (CA1) region. However, we observed varying degrees of hippocampal cell loss among animals. Here, we report for the first time an anomaly of the aortic arch in some Rhesus macaques that appears as a key surgical factor in ensuring the success of the TGI model in this particular NHP. Ele...
Source: Molecular Medicine - June 10, 2009 Category: Molecular Biology Authors: Hara K, Yasuhara T, Maki M, Matsukawa N, Yu G, Xu L, Tambrallo L, Rodriguez NA, Stern DM, Yamashima T, Buccafusco JJ, Kawase T, Hess DC, Borlongan CV Tags: Brain Res Source Type: journals

Further characterization of the new microdeletion syndrome of 16p11.2-p12.2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Using aCGH, we have identified a pericentromeric deletion, spanning about 8.2 Mb, within 16p11.2-p12.2 in a patient with developmental delay (DD) and dysmorphic features. This deletion arose de novo and is flanked by segmental duplications. The proposita was the only child of healthy nonconsanguineous parents, born after an uneventful pregnancy, at 40 weeks gestation, by normal delivery. She was referred to us at age 3 10/12 years for evaluation of DD and absent speech. On examination, there were a flat face; low-set, posteriorly rotated ears; high-arched palate; hypotonic face; right single palmar crease; long, thin f...
Source: Molecular Medicine - June 1, 2009 Category: Molecular Biology Authors: Battaglia A, Novelli A, Bernardini L, Igliozzi R, Parrini B Tags: Am J Med Genet A Source Type: journals

Vascular enhancement in early dynamic liver MR imaging in an animal model: comparison of two injection regimen and two different doses Gd-EOB-DTPA (gadoxetic acid) with standard Gd-DTPA.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSION: Despite the lower amount of gadolinium in the standard dose of Gd-EOB-DTPA, the results showed that the arterial enhancement in Gd-EOB-DTPA-enhanced dynamic liver MRI was comparable to Gd-DTPA. This result can be explained mainly by the higher relaxivity. Choosing a lower injection rate additionally supported to compensate for the lower injection volume by stretching the bolus without decreasing the peak. In this respect, an injection rate of 1 mL/s showed better results with regard to the arterial enhancement compared with 2 mL/s. PMID: 19462484 [PubMed - in process] (Source: Molecular Medicine)
Source: Molecular Medicine - June 1, 2009 Category: Molecular Biology Authors: Zech CJ, Vos B, Nordell A, Urich M, Blomqvist L, Breuer J, Reiser MF, Weinmann HJ Tags: Invest Radiol Source Type: journals

Bone marrow-derived endothelial progenitor cells contribute to the angiogenic switch in tumor growth and metastatic progression.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Emerging evidence indicates that bone marrow (BM)-derived endothelial progenitor cells (EPCs) contribute to angiogenesis-mediated growth of certain tumors in mice and human. EPCs regulate the angiogenic switch via paracrine secretion of proangiogeneic growth factors and by direct luminal incorporation into sprouting nascent vessels. While the contributions of EPCs to neovessel formation in spontaneous and transplanted tumors and to the metastatic transition have been reported to be relatively low, remarkably, specific EPC ablation in vivo has resulted in severe angiogenesis inhibition and impaired primary and metastati...
Source: Molecular Medicine - May 19, 2009 Category: Molecular Biology Authors: Gao D, Nolan D, McDonnell K, Vahdat L, Benezra R, Altorki N, Mittal V Tags: Biochim Biophys Acta Source Type: journals

Nerve injection of viral vectors efficiently transfers transgenes into motor neurons and delivers RNAi therapy against ALS.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report, we tested RNAi therapy in a mouse model for amyotrophic lateral sclerosis (ALS), which causes motor neuron degeneration, paralysis and death. We used a transgenic model that overexpresses mutant Cu, Zn superoxide dismutase (SOD1G93A), which causes ALS by a gained toxic property. We delivered RNAi using recombinant adenovirus (RAd) and adeno-associated virus serotype 2 (AAV2). We compared the efficiency of RNAi delivery between injecting the viral vectors into muscle and into nerve, and found that nerve injetion is more efficient in delivering RNAi to motor neurons. Based on this data, we conducted therapeut...
Source: Molecular Medicine - April 3, 2009 Category: Molecular Biology Authors: Wu R, Wang H, Xia XG, Zhou H, Liu C, Castro MG, Xu Z Tags: Antioxid Redox Signal Source Type: journals

Differentiation of bone marrow derived endothelial progenitor cells is shifted into a pro-inflammatory phenotype by hyperglycemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bone marrow (BM)-derived endothelial progenitor cells (EPC) contribute to vascular maintenance by participating in angiogenesis, re-endothelialization and remodeling. Myeloid progenitor cells in the BM are functionally and quantitatively an important precursor pool for cells that contribute to these processes. However, these precursor pools in the BM also give rise to important effector cells of the innate immune system such as macrophages and dendritic cells. We hypothesized that the disturbed repair responses that are being observed in diabetes mellitus are also related to an effect on functional and differentiation ...
Source: Molecular Medicine - March 19, 2009 Category: Molecular Biology Authors: Loomans CJ, van Haperen R, Duijs JM, Verseyden C, de Crom R, Leenen PJ, Drexhage HA, de Boer HC, de Koning EJ, Rabelink TJ, Staal FJ, van Zonneveld AJ Tags: Mol Med Source Type: journals

IGF-1 Expression in Infarcted Myocardium and MGF E Peptide Actions on Rat Cardiomyocytes, In Vitro.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Insulin-like growth factor-1 (IGF-1) expression is implicated in myocardial pathophysiology and two IGF-1 mRNA splice variants have been detected in rodents, namely the IGF-1Ea and the mechano-growth factor (MGF). Consequently, we have investigated the expression pattern of the IGF-1 gene transcripts in rat myocardium after left anterior descending coronary artery ligation-induced myocardial infarction (1hr up to 8wks). In addition, we characterized IGF-1 and MGF E peptide action and their respective signaling in H9C2 myocardial-like cells, in vitro. IGF-1Ea and MGF expression were significantly increased, both at tran...
Source: Molecular Medicine - March 19, 2009 Category: Molecular Biology Authors: Stavropoulou A, Halapas A, Sourla A, Philippou A, Papageorgiou E, Papalois A, Koutsilieris M Tags: Mol Med Source Type: journals

Genome Wide Association Study of Determinants of Anti-cyclic Citrullinated Peptide Antibody Titer in Adults with Rheumatoid Arthritis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We carried out a genome wide association study of genetic predictors of anti-CCP level in 531 self-reported non-Hispanic Caucasian RA patients enrolled in the Brigham Rheumatoid Arthritis Sequential Study (BRASS). For replication we then analyzed 289 SNPs with p<0.001 in BRASS in an independent population of 849 RA patients from the North American Rheumatoid Arthritis Consortium (NARAC). BRASS and NARAC samples were genotyped using the Affymetrix 100K and Illumina 550K platforms respectively. Association between SNPs and anti-CCP titer was tested using general linear models. The 5 most significant SNPs from BRASS we...
Source: Molecular Medicine - March 14, 2009 Category: Molecular Biology Authors: Cui J, Taylor K, Destefano A, Criswell L, Izmailova E, Alex P, Roubenoff R, Plenge R, Weinblatt M, Shadick N, Karlson E Tags: Mol Med Source Type: journals

A novel recombinant vaccinia virus expressing the human norepinephrine transporter retains oncolytic potential and facilitates deep tissue imaging.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Noninvasive and repetitive monitoring of a virus in target tissues and/or specific organs of the body is highly desirable for the development of safe and efficient cancer virotherapeutics. We have previously shown that the oncolytic vaccinia virus GLV-1h68 can target and eradicate human tumors in mice and that its therapeutic effects can be monitored using optical imaging. Here, we report on the development of a novel recombinant vaccinia virus (VACV) GLV-1h99, a derivative of GLV-1h68, which was constructed to carry the human norepinephrine transporter gene (hNET) under the VACV synthetic early promoter placed at the ...
Source: Molecular Medicine - March 14, 2009 Category: Molecular Biology Authors: Chen N, Zhang Q, Yu YA, Stritzker J, Brader P, Schirbel A, Samnick S, Serganova I, Blasberg R, Fong Y, Szalay AA Tags: Mol Med Source Type: journals

Analysis Association Between Mitochondrial Genome Instability and Xenobiotic Metabolizing Genes in Human Breast Cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to determine the existence of association between the genetic polymorphisms of metabolizing genes GSTM-1, GSTT-1 and NAT-2, and the presence of mtGI in breast cancer cases. 94 pairs of tumoral/non-tumoral breast cancer samples were analyzed. A 40,42% of mtGI were found in the samples by analysis of two D-loop region markers, a (CA)n mtMS starting at the 514-bp position and 4 informative MnlI sites between the 16,108-16,420-bp. GSTM-1 Null genotype had showed a significant association with mtGI presence (chi2: 7.62; p: 0.006) in breast cancer cases; moreover, these genotype were also related to...
Source: Molecular Medicine - March 14, 2009 Category: Molecular Biology Authors: Pavicic WH, Laguens M, Richard SM Tags: Mol Med Source Type: journals

Invited Editorial: Oxygen-conserving implications of the trigemino-cardiac reflex in the brain: The molecular basis of neuroprotection?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19287512 [PubMed - as supplied by publisher] (Source: Molecular Medicine)
Source: Molecular Medicine - March 14, 2009 Category: Molecular Biology Authors: Schaller B, Sandu N, Cornelius J, Filis A, Perez-Pinzon M, Tags: Mol Med Source Type: journals

Molecular determinants of response to matuzumab in combination with paclitaxel for patients with advanced non-small cell lung cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Antibodies targeting epidermal growth factor receptor (EGFR) have proven to be effective in patients with non-small cell lung cancer (NSCLC) that express EGFR. We recently published a phase I study of weekly matuzumab plus paclitaxel. This therapy was well tolerated and showed clinical responses in the majority of patients. Although matuzumab displays potent antitumor activity in some patients, not all patients respond well to treatment. Whether dysregulation of EGFR-mediated pathways precludes or sensitizes cells to paclitaxel is unknown. We sought to determine molecular predictive factors for therapy response in a ph...
Source: Molecular Medicine - March 1, 2009 Category: Molecular Biology Authors: Schittenhelm MM, Kollmannsberger C, Oechsle K, Harlow A, Morich J, Honecker F, Kurek R, Störkel S, Kanz L, Corless CL, Wong KK, Bokemeyer C, Heinrich MC Tags: Mol Cancer Ther Source Type: journals

Comparative Mitochondrial Proteomic Analysis of Raji Cells Exposed to Adriamycin.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to investigate ADR about its antineoplastic activity, drug resistance and unexpected toxicity on non-Hodgkin's lymphoma (NHL) Raji cells at mitochondrial proteomic level. The alterations of the mitochondrial proteome of Raji cells treated with ADR were analyzed by 2D-DIGE coupled with LTQ-ESI-MS/MS. The altered patterns of three identified proteins were validated by western blot and analyzed by pathway studio software. The results showed that 34 proteins were found down-regulated and 3 proteins up-regulated when the study group was compared with the control group. The differentially expressed proteins dist...
Source: Molecular Medicine - February 11, 2009 Category: Molecular Biology Authors: Jiang YJ, Sun Q, Fang XS, Wang X Tags: Mol Med Source Type: journals

Activation of the cholinergic anti-inflammatory pathway reduces ricin-induced mortality and organ failure in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, these data demonstrate that activation of the cholinergic anti-inflammatory pathway may prove beneficial in cases of ricin poisoning reducing organ damage and delaying mortality allowing for a greater chance of survival. PMID: 19209239 [PubMed - as supplied by publisher] (Source: Molecular Medicine)
Source: Molecular Medicine - February 11, 2009 Category: Molecular Biology Authors: Mabley JG, Pacher P, Szabo C Tags: Mol Med Source Type: journals

Microvessel length density, total length, and length per neuron in five subcortical regions in schizophrenia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recent studies (Prabakaran et al. in Mol Psychiat 9:684-697, 2004; Hanson and Gottesman in BMC Med Genet 6:7, 2005; Harris et al. in PLoS ONE 3:e3964, 2008) have suggested that microvascular abnormalities occur in the brains of patients with schizophrenia. To assess the integrity of the microvasculature in subcortical brain regions in schizophrenia, we investigated the microvessel length density, total microvessel length, and microvessel length per neuron using design-based stereologic methods in the caudate nucleus, putamen, nucleus accumbens, mediodorsal nucleus of the thalamus, and lateral nucleus of the amygdala in...
Source: Molecular Medicine - February 6, 2009 Category: Molecular Biology Authors: Kreczmanski P, Heinsen H, Mantua V, Woltersdorf F, Masson T, Ulfig N, Schmidt-Kastner R, Korr H, Steinbusch HW, Hof PR, Schmitz C Tags: Acta Neuropathol Source Type: journals

Diagnostic value of Zinc Protoporphyrin in a screening strategy for alpha-thalassaemia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ABSTRACT The definitive diagnosis of alpha-thalassaemia involves detection of a deletion of one or more Hla alleles that encode the alpha-chains of Hb (hemoglobin). To determine whether DNA analysis is indicated, screening tests such as mean corpuscular volume (MCV) and Hb typing are employed. alpha-Thalassaemia often correlates with normal or low HbA2 values. Zinc protoporphyrin (ZPP) is usually high in ferropenic anemia or lead-poisoning and is normal or slightly raised in ss-thalassaemia. Therefore, ZPP is currently used as a marker to discriminate between ferropenic anemia and beta-thalassaemia. We investigated the...
Source: Molecular Medicine - January 29, 2009 Category: Molecular Biology Authors: Sardón Estévez N, Herruer MH, Jansen R, Bergkamp FJ, Gorgels JP Tags: Eur J Haematol Source Type: journals