Exosomal microRNAs as potential biomarkers and therapeutic targets in corneal diseases
Mol Vis. 2024 Mar 15;30:92-106. eCollection 2024.ABSTRACTExosomes are a subtype of extracellular vesicle (EV) that are released and found in almost all body fluids. Exosomes consist of and carry a variety of bioactive molecules, including genetic information in the form of microRNAs (miRNAs). miRNA, a type of small non-coding RNA, plays a key role in regulating genes by suppressing their translation. miRNAs are often disrupted in the pathophysiology of different conditions, including eye disease. The stability and easy detectability of exosomal miRNAs in body fluids make them promising biomarkers for the diagnosis of diffe...
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Swati Arora Nagendra Verma Source Type: research
EphB1 causes retinal damage through inflammatory pathways in the retina and retinal M üller cells
CONCLUSIONS: EphB1 is increased in the retinas of diabetic humans and mice and in high glucose-treated Müller cells. This increase leads to inflammatory proteins. EphB1 also enhanced retinal damage in response to I/R. Taken together, inhibition of EphB1 may offer a new therapeutic option for diabetic retinopathy.PMID:38601015 | PMC:PMC11006007 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Li Liu Youde Jiang Mohamed Al-Shabrawey Xiaobai Ren Sui Wang Jena J Steinle Source Type: research
Two novel non-coding single nucleotide variants in the DNase1 hypersensitivity site of < em > PRDM13 < /em > causing North Carolina macular dystrophy in Korea
CONCLUSION: We identified two novel NCMD variants in the Korean population and further validated the regulatory role of the DNase1 hypersensitivity site upstream of PRDM13.PMID:38601016 | PMC:PMC11006008 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Yuri Seo Kwangsic Joo Junwon Lee Amber Diaz Sohyun Jang Timothy J Cherry Kinga M Bujakowska Jinu Han Se Joon Woo Kent W Small Source Type: research
Microstructure of the corneal endothelial transition zone in different laboratory animals
CONCLUSIONS: Although the transition zone was not identified clearly, irregular cell morphology and loss of cell-cell contact were observed in all animal corneal endothelial cells. The proliferative capacity and the presence of progenitor cells were confirmed in the transition zone, especially in the rabbit cornea.PMID:38601017 | PMC:PMC11006004 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Jun Seob Lee So Young Lee Hee Seung Chin Na Rae Kim Ji Won Jung Source Type: research
Review: Mechanisms of TIMP-3 accumulation and pathogenesis in Sorsby fundus dystrophy
Mol Vis. 2024 Mar 3;30:74-91. eCollection 2024.ABSTRACTSorsby fundus dystrophy (SFD) is a rare, inherited form of macular degeneration caused by mutations in the gene encoding tissue inhibitor of metalloproteinases 3 (TIMP-3). There are 21 mutations currently associated with SFD, with some variants (e.g., Ser179Cys, Tyr191Cys, and Ser204Cys) having been studied much more than others. We review what is currently known about the identified SFD variants in terms of their dimerization, metalloproteinase inhibition, and impact on angiogenesis, with a focus on disparities between reports and areas requiring further study. We als...
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Jacob H J Betts Linda Troeberg Source Type: research
The generation and characterization of a transgenic zebrafish line with lens-specific Cre expression
CONCLUSIONS: We established a zTg(cryaa:Cre-cryaa:EGFP) zebrafish line that can specifically express an active Cre recombinase in lens tissues. This transgenic zebrafish line can be used as a tool to specifically manipulate a gene in zebrafish lenses.PMID:38601019 | PMC:PMC11006009 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Xuyan Peng Xiaolin Jia Guohui Shang Mengjiao Xue Mingjun Jiang Dandan Chen Fengyan Zhang Yanzhong Hu Source Type: research
Uveal melanoma cell lines Mel270 and 92.1 exhibit a mesenchymal phenotype and sensitivity to the cytostatic effects of transforming growth factor beta in vitro
CONCLUSIONS: Our findings indicate that UM cells are sensitive to the two arms of TGF-β signaling, which suggests that targeting the TGF-β pathway could be challenging in UM and would require a precise selection of patients in which only the prometastatic arm of TGF-β is activated.PMID:38601020 | PMC:PMC11006005 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Coralie Doudnikoff Delphine Leclerc Ga ëlle Angenard David Gilot C édric Coulouarn Frederic Mouriaux Source Type: research
Exosomal microRNAs as potential biomarkers and therapeutic targets in corneal diseases
Mol Vis. 2024 Mar 15;30:92-106. eCollection 2024.ABSTRACTExosomes are a subtype of extracellular vesicle (EV) that are released and found in almost all body fluids. Exosomes consist of and carry a variety of bioactive molecules, including genetic information in the form of microRNAs (miRNAs). miRNA, a type of small non-coding RNA, plays a key role in regulating genes by suppressing their translation. miRNAs are often disrupted in the pathophysiology of different conditions, including eye disease. The stability and easy detectability of exosomal miRNAs in body fluids make them promising biomarkers for the diagnosis of diffe...
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Swati Arora Nagendra Verma Source Type: research
EphB1 causes retinal damage through inflammatory pathways in the retina and retinal M üller cells
CONCLUSIONS: EphB1 is increased in the retinas of diabetic humans and mice and in high glucose-treated Müller cells. This increase leads to inflammatory proteins. EphB1 also enhanced retinal damage in response to I/R. Taken together, inhibition of EphB1 may offer a new therapeutic option for diabetic retinopathy.PMID:38601015 | PMC:PMC11006007 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Li Liu Youde Jiang Mohamed Al-Shabrawey Xiaobai Ren Sui Wang Jena J Steinle Source Type: research
Two novel non-coding single nucleotide variants in the DNase1 hypersensitivity site of < em > PRDM13 < /em > causing North Carolina macular dystrophy in Korea
CONCLUSION: We identified two novel NCMD variants in the Korean population and further validated the regulatory role of the DNase1 hypersensitivity site upstream of PRDM13.PMID:38601016 | PMC:PMC11006008 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Yuri Seo Kwangsic Joo Junwon Lee Amber Diaz Sohyun Jang Timothy J Cherry Kinga M Bujakowska Jinu Han Se Joon Woo Kent W Small Source Type: research
Microstructure of the corneal endothelial transition zone in different laboratory animals
CONCLUSIONS: Although the transition zone was not identified clearly, irregular cell morphology and loss of cell-cell contact were observed in all animal corneal endothelial cells. The proliferative capacity and the presence of progenitor cells were confirmed in the transition zone, especially in the rabbit cornea.PMID:38601017 | PMC:PMC11006004 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Jun Seob Lee So Young Lee Hee Seung Chin Na Rae Kim Ji Won Jung Source Type: research
Review: Mechanisms of TIMP-3 accumulation and pathogenesis in Sorsby fundus dystrophy
Mol Vis. 2024 Mar 3;30:74-91. eCollection 2024.ABSTRACTSorsby fundus dystrophy (SFD) is a rare, inherited form of macular degeneration caused by mutations in the gene encoding tissue inhibitor of metalloproteinases 3 (TIMP-3). There are 21 mutations currently associated with SFD, with some variants (e.g., Ser179Cys, Tyr191Cys, and Ser204Cys) having been studied much more than others. We review what is currently known about the identified SFD variants in terms of their dimerization, metalloproteinase inhibition, and impact on angiogenesis, with a focus on disparities between reports and areas requiring further study. We als...
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Jacob H J Betts Linda Troeberg Source Type: research
The generation and characterization of a transgenic zebrafish line with lens-specific Cre expression
CONCLUSIONS: We established a zTg(cryaa:Cre-cryaa:EGFP) zebrafish line that can specifically express an active Cre recombinase in lens tissues. This transgenic zebrafish line can be used as a tool to specifically manipulate a gene in zebrafish lenses.PMID:38601019 | PMC:PMC11006009 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Xuyan Peng Xiaolin Jia Guohui Shang Mengjiao Xue Mingjun Jiang Dandan Chen Fengyan Zhang Yanzhong Hu Source Type: research
Uveal melanoma cell lines Mel270 and 92.1 exhibit a mesenchymal phenotype and sensitivity to the cytostatic effects of transforming growth factor beta in vitro
CONCLUSIONS: Our findings indicate that UM cells are sensitive to the two arms of TGF-β signaling, which suggests that targeting the TGF-β pathway could be challenging in UM and would require a precise selection of patients in which only the prometastatic arm of TGF-β is activated.PMID:38601020 | PMC:PMC11006005 (Source: Molecular Vision)
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Coralie Doudnikoff Delphine Leclerc Ga ëlle Angenard David Gilot C édric Coulouarn Frederic Mouriaux Source Type: research
Exosomal microRNAs as potential biomarkers and therapeutic targets in corneal diseases
Mol Vis. 2024 Mar 15;30:92-106. eCollection 2024.ABSTRACTExosomes are a subtype of extracellular vesicle (EV) that are released and found in almost all body fluids. Exosomes consist of and carry a variety of bioactive molecules, including genetic information in the form of microRNAs (miRNAs). miRNA, a type of small non-coding RNA, plays a key role in regulating genes by suppressing their translation. miRNAs are often disrupted in the pathophysiology of different conditions, including eye disease. The stability and easy detectability of exosomal miRNAs in body fluids make them promising biomarkers for the diagnosis of diffe...
Source: Molecular Vision - April 11, 2024 Category: Molecular Biology Authors: Swati Arora Nagendra Verma Source Type: research
