Molecular and Cellular BiochemistryThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

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Impact of template overhang-binding region of HIV-1 RT on the binding and orientation of the duplex region of the template-primer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Fingers domain of HIV-1 RT is one of the constituents of the dNTP-binding pocket that is involved in binding of both dNTP and the template-primer. In the ternary complex of HIV-1 RT, two residues Trp-24 and Phe-61 located on the beta1 and beta3, respectively, are seen interacting with N + 1 to N + 3 nucleotides in the template overhang. We generated nonconservative and conservative mutant derivatives of these residues and examined their impact on the template-primer binding and polymerase function of the enzyme. We noted that W24A, F61A, and F61Y and the double mutant (W24A/F61A) were significantly affected in their ab...
Source: Molecular and Cellular Biochemistry - November 17, 2009 Category: Biochemistry Authors: Upadhyay AK, Talele TT, Pandey VN Tags: Mol Cell Biochem Source Type: journals

Ca(2+)-modulated membrane guanylate cyclase in the testes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To date, the calcium-regulated membrane guanylate cyclase Rod Outer Segment Guanylate Cyclase type 1 (ROS-GC1) transduction system in addition to photoreceptors is known to be expressed in three other types of neuronal cells: in the pinealocytes, mitral cells of the olfactory bulb and the gustatory epithelium of tongue. Very recent studies from our laboratory show that expression of ROS-GC1 is not restricted to the neuronal cells; the male gonads and the spermatozoa also express ROS-GC1. In this presentation, the authors review the existing information on the localization and function of guanylate cyclase with special ...
Source: Molecular and Cellular Biochemistry - November 15, 2009 Category: Biochemistry Authors: Jankowska A, Warchol JB Tags: Mol Cell Biochem Source Type: journals

Diversity of sensory guanylate cyclases in teleost fishes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Teleost fishes like medaka fish (Oryzias latipes), zebrafish (Danio rerio), and pufferfish (Fugu rubripes) contain in their genomes a larger number of guanylate cyclases and guanylate cyclase-activating proteins than mammals. Based on amino acid sequence alignments a group of transmembrane sensory guanylate cyclases can be identified, which are mainly if not exclusively expressed in sensory organs like the retina and olfactory tissue. Retina specific guanylate cyclases and guanylate cyclase-activating proteins in the zebrafish show dynamic changes in their spatial-temporal expression patterns and transcripts of the cor...
Source: Molecular and Cellular Biochemistry - November 14, 2009 Category: Biochemistry Authors: Rätscho N, Scholten A, Koch KW Tags: Mol Cell Biochem Source Type: journals

Adaptive immune responses during Shigella dysenteriae type 1 infection: an in vitro stimulation with 57 kDa major antigenic OMP in the presence of anti-CD3 antibody.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An effort was made to understand the role of the 57 kDa major antigenic fraction of Shigella outer membrane protein (OMP) in the presence of T-cell antigen receptor in activation of adaptive immune responses of the cell mediated immune (CMI) restored patients. The expression of HLA-DR/CD4 out of CD3(+) T-cells was significantly dominant over the HLA-DR/CD8 and comparable to unstimulated cells of infected or healthy controls. CD4(+) T-cell activation together with HLA-DR is associated with the expression of CD25(+) (IL2Ralpha) for IL-2 growth factors with decreased IL-4 levels, required for maintaining the homeostasis o...
Source: Molecular and Cellular Biochemistry - November 14, 2009 Category: Biochemistry Authors: Bagchi AK, Sinha AK, Adhikari R, Mukherjee J Tags: Mol Cell Biochem Source Type: journals

Dietary fat and apolipoprotein genotypes modulate plasma lipoprotein levels in Brazilian elderly women.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to investigate the impact of apolipoprotein E, A5, and B genotypes and dietary intake on lipid profile in a sample of elderly women in Brazil. Two hundred and fifty-two women (60 years or older) living in the outskirts of the Brazilian Federal District underwent clinical and laboratory assessments to characterize glycemic and lipidemic variables, and also to exclude confounding factors (smoking, drinking, hormone replacement, cognitive impairment, physical activity). Three-day food records were used to determine usual dietary intake, whereas genotypic evaluations were in accordance to established methodolo...
Source: Molecular and Cellular Biochemistry - November 14, 2009 Category: Biochemistry Authors: Paula RS, Souza VC, Benedet AL, Souza ER, Toledo JO, Moraes CF, Gomes L, Alho CS, Córdova C, Nóbrega OT Tags: Mol Cell Biochem Source Type: journals

Identification of novel PTEN-binding partners: PTEN interaction with fatty acid binding protein FABP4.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PTEN is a tumor suppressor with dual protein and lipid-phosphatase activity, which is frequently deleted or mutated in many human advanced cancers. Recent studies have also demonstrated that PTEN is a promising target in type II diabetes and obesity treatment. Using C-terminal PTEN sequence in pEG202-NLS as bait, yeast two-hybrid screening on Mouse Embryo, Colon Cancer, and HeLa cDNA libraries was carried out. Isolated positive clones were validated by mating assay and identified through automated DNA sequencing and BLAST database searches. Sequence analysis revealed a number of PTEN-binding proteins linking this phosp...
Source: Molecular and Cellular Biochemistry - November 13, 2009 Category: Biochemistry Authors: Gorbenko O, Panayotou G, Zhyvoloup A, Volkova D, Gout I, Filonenko V Tags: Mol Cell Biochem Source Type: journals

MS80, a novel sulfated polysaccharide, inhibits CD40-NF-kappaB pathway via targeting RIP2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the previous studies, MS80 was found to be able to inhibit the pulmonary fibrosis. However, the target of MS80 remains unclear. To determine the target and the anti-fibrosis mechanisms of MS80, affinity column, MALDI-TOF-MS/MS, co-immunoprecipitation, and co-localization were used. The results showed that MS80 targeting protein was receptor interacting protein 2 (RIP2), which was further confirmed by co-immunoprecipitation and co-localization. Moreover, MS80 inhibited the CD40 ligation-induced NF-kappaB activation, and subsequently inflammatory cytokines secretion, the collagen synthesis, and the excessive prolifera...
Source: Molecular and Cellular Biochemistry - November 13, 2009 Category: Biochemistry Authors: Du X, Jiang S, Liu H, Xin X, Li J, Geng M, Jiang H Tags: Mol Cell Biochem Source Type: journals

Hepsin activates prostasin and cleaves the extracellular domain of the epidermal growth factor receptor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The epithelial extracellular serine protease activation cascade involves matriptase (PRSS14) and prostasin (PRSS8), capable of modulating epidermal growth factor receptor (EGFR) signaling. Matriptase activates prostasin by cleaving in the amino-terminal pro-peptide region of prostasin, presumably at the Arg residue of position 44 (R44) of the full-length human prostasin. Using an Arg-to-Ala mutant (R44A) human prostasin, we showed in this report that the cleavage of prostasin by matriptase is at Arg44. This prostasin proteolytic activation site is also cleaved by hepsin (TMPRSS1) to produce active prostasin capable of ...
Source: Molecular and Cellular Biochemistry - November 13, 2009 Category: Biochemistry Authors: Chen M, Chen LM, Lin CY, Chai KX Tags: Mol Cell Biochem Source Type: journals

A common polymorphism in the promoter of UCP2 is associated with obesity and hyperinsulenemia in northern Indians.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our results suggest that the -866 AA genotype and A allele of the UCP2 gene is associated with obesity and A allele associated with hyperinsulinemia in obese subjects. PMID: 19908126 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - November 12, 2009 Category: Biochemistry Authors: Srivastava N, Prakash J, Lakhan R, Agarwal CG, Pant DC, Mittal B Tags: Mol Cell Biochem Source Type: journals

Localization of erythropoietin in and around growing cartilage.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Erythropoietin (EPO) is known to be a hematopoietic growth factor and a regulator of red blood cell production. Recently, EPO has also been reported to function as a tissue-protective cytokine and as an angiogenesis promoting factor. EPO is mainly regulated by hypoxia through the action of hypoxia inducible factors (HIF-1alpha and HIF-2alpha). The localization of the EPO protein and the HIF-2alpha protein were immunohistochemically analyzed in developing porcine embryos. Both proteins were localized in developing cartilage tissue. HIF-2alpha and EPO protein were expressed in the peripheral chondrocytes of cartilage anl...
Source: Molecular and Cellular Biochemistry - November 12, 2009 Category: Biochemistry Authors: De Spiegelaere W, Cornillie P, Van den Broeck W Tags: Mol Cell Biochem Source Type: journals

Role of genetic polymorphism of estrogen receptor-alpha gene and risk of prostate cancer in north Indian population.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Present study depicted the role of polymorphisms in estrogen receptor-alpha gene in association with prostate cancer in north Indian population. The study was performed on 157 cases of prostate cancer, 170 cases of BPH, and 170 healthy Indian males diagnosed with prostate cancer and benign prostatic hyperplasia (BPH) and healthy males as controls. Determination of polymorphism in the ER-alpha gene was done by polymerase chain reaction followed by restriction fragment length polymorphism (RFLP) analysis with PvuII and XbaI enzymes. An association was observed between PvuII polymorphism of ER-alpha gene and that of prost...
Source: Molecular and Cellular Biochemistry - November 11, 2009 Category: Biochemistry Authors: Gupta L, Thakur H, Sobti RC, Seth A, Singh SK Tags: Mol Cell Biochem Source Type: journals

The male-determining gene SRY is a hybrid of DGCR8 and SOX3, and is regulated by the transcription factor CP2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In mammals, sex is determined by the presence or absence of the Y chromosome that bears a male-dominant sex-determining gene SRY, which switches the differentiation of gonads into male testes. The molecular signaling mechanism turning on the switch, however, has remained unclear for 18 years since the identification of the gene. Here, we describe how this gene emerged and started to work. From amino acid homology, we realized that SRY is a hybrid gene between a portion of the first exon of DiGeorge syndrome critical region gene 8 (DGCR8) and the high-mobility group (HMG) box of SRY box-3 (SOX3) gene. We identified the ...
Source: Molecular and Cellular Biochemistry - November 10, 2009 Category: Biochemistry Authors: Sato Y, Shinka T, Sakamoto K, Ewis AA, Nakahori Y Tags: Mol Cell Biochem Source Type: journals

Novel function of RECS1 as a negative regulator of TNF-alpha-induced NF-kappaB activation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Shear stress plays a significant role in vascular remodeling, in which process tumor necrosis factor-alpha (TNF-alpha)-induced activation of NF-kappaB is generally considered to be involved. RECS1 (responsive to centrifugal force and shear stress gene 1) was first reported to be a shear stress responsive gene and RECS1 knockout mice at old age were prone to cystic medium degeneration (CMD) and aortic dilation, accompanied by enhanced expression of matrix metalloproteinase 9 (MMP-9). RECS1 was here identified as an interacting protein of tumor necrosis factor receptor 1 (TNFR1) and negatively regulated TNF-alpha-induced...
Source: Molecular and Cellular Biochemistry - November 8, 2009 Category: Biochemistry Authors: Liao Z, Wu M, Chen X Tags: Mol Cell Biochem Source Type: journals

Study of possible interactions of tubulin, microtubular network, and STOP protein with mitochondria in muscle cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We studied possible connections of tubulin, microtubular system, and microtubular network stabilizing STOP protein with mitochondria in rat and mouse cardiac and skeletal muscles by confocal microscopy and oxygraphy. Intracellular localization and content of tubulin was found to be muscle type-specific, with high amounts in oxidative muscles, and much lower in glycolytic skeletal muscle. STOP protein localization and content in muscle cells was also muscle type-specific. In isolated heart mitochondria, addition of 1 muM tubulin heterodimer increased apparent K (m) for ADP significantly. Dissociation of microtubular sys...
Source: Molecular and Cellular Biochemistry - November 4, 2009 Category: Biochemistry Authors: Guerrero K, Monge C, Brückner A, Puurand U, Kadaja L, Käämbre T, Seppet E, Saks V Tags: Mol Cell Biochem Source Type: journals

Inhibition of neuronal cell death after retinoic acid-induced down-regulation of P2X(7) nucleotide receptor expression.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Apoptosis is a major mechanism for cell death in the nervous system during development. P2X(7) nucleotide receptors are ionotropic ATP receptors that mediate cell death under pathological conditions. We developed an in vitro protocol to investigate the expression and functional responses of P2X(7) nucleotide receptors during retinoic acid (RA)-induced neuronal differentiation of human SH-SY5Y neuroblastoma cells. Neuronal differentiation was examined measuring cellular growth arrest and neuritic processes elongation. We found that SH-SY5Y cells treated for 5 days with RA under low serum content exhibited a neuron-like ...
Source: Molecular and Cellular Biochemistry - November 1, 2009 Category: Biochemistry Authors: Orellano EA, Rivera OJ, Chevres M, Chorna NE, González FA Tags: Mol Cell Biochem Source Type: journals

The expression of HMGB1 protein and its receptor RAGE in human malignant tumors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
High Mobility Group Box 1 (HMGB1) is a nuclear non-histone protein discovered to be released in the extracellular medium as a response to various stimuli and implicated in cancerogenesis. High HMGB1 levels are reported in a variety of tumor types, but there are few data relating HMGB1 to the histological grade or to a particular cell type and cellular localization. We studied the expression of HMGB1 protein in malignant human tumors of different differentiation level and in tumor metastasis. In all tumor tissues, the protein level is elevated. In moderately differentiated carcinomas, the localization of the protein is ...
Source: Molecular and Cellular Biochemistry - October 30, 2009 Category: Biochemistry Authors: Kostova N, Zlateva S, Ugrinova I, Pasheva E Tags: Mol Cell Biochem Source Type: journals

Functional effects of protein kinases and peroxynitrite on cardiac carnitine palmitoyltransferase-1 in isolated mitochondria.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We have previously shown that metoprolol can inhibit carnitine palmitoyltransferase-1 catalytic activity and decrease its malonyl CoA sensitivity within 30 min, suggesting the importance of a covalent modification. The aim of this study was to characterize the effects of PTMs on CPT-1 in the heart. Mitochondria were isolated from the hearts of male Wistar rats and incubated with kinases of interest (protein kinase A, CAMK-II, p38 MAPK, Akt) or with peroxynitrite and sodium nitroprusside. PKA decreased CPT-1 malonyl CoA sensitivity, associated with phosphorylation of CPT-1A, whereas CAMK-II increased malonyl CoA sensiti...
Source: Molecular and Cellular Biochemistry - October 28, 2009 Category: Biochemistry Authors: Sharma V, Abraham T, So A, Allard MF, McNeill JH Tags: Mol Cell Biochem Source Type: journals

Use of pifithrin to inhibit p53-mediated signalling of TNF in dystrophic muscles of mdx mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigates the role that p53 plays in TNF-mediated necrosis of dystrophic myofibres by inhibiting p53 using pifithrin-alpha and three pifithrin-beta analogues. Tissue culture studies using C2C12 myoblasts established that pifithrin-alpha was toxic to differentiating myoblasts at concentrations greater than 10 muM. While non-toxic concentrations of pifithrin-alpha did not prevent the TNF-mediated inhibition of myoblast differentiation, Western blots indicated that nuclear levels of p53 were higher in TNF-treated myoblasts indicating that TNF does elevate p53. In contrast, in vivo studies in adult mdx mice showe...
Source: Molecular and Cellular Biochemistry - October 27, 2009 Category: Biochemistry Authors: Waters FJ, Shavlakadze T, McIldowie MJ, Piggott MJ, Grounds MD Tags: Mol Cell Biochem Source Type: journals

c-Jun-NH(2) terminal kinase (JNK)-mediates AP-1 activation by thioredoxin: phosphorylation of cJun, JunB, and Fra-1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Thioredoxin (Trx) is a small ubiquitous protein, which has been shown to be involved in redox-dependent cellular functions. In this article, we demonstrate that the increased level of Trx induces AP-1 DNA binding in a redox-dependent manner by activating JNK subgroup of MAPKs. The majority of AP-1 DNA binding complex was found to be composed of cJun, JunB, and Fra-1. Increased expression of Trx resulted in phosphorylation of cJun, Jun B, and Fra-1. Further, increased expression of Trx induced the phosphorylation of MKK4 and MKK7 which are upstream kinases of the JNK signaling cascade. In co-transfection studies, AP-1-d...
Source: Molecular and Cellular Biochemistry - October 27, 2009 Category: Biochemistry Authors: Das KC, Muniyappa H Tags: Mol Cell Biochem Source Type: journals

Phospholipase D modulation by ceramide in senescence.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the source of changes in PLD activity in senescent human umbilical vein endothelial cells and in human diploid fibroblasts. Using fractionated cell components we found that the cytosolic components were necessary for maximum PLD activation. In comparison to low-passage cells, senescent cells showed a profound lack of PLD activatability. By recombining fractionated components from senescent and low-passage cells, we found that in senescence the membrane component is defective in activating PLD implicating either the PLD enzyme itself or its interaction with PKC and/or ARF. The sphingolipid ceramide has been impl...
Source: Molecular and Cellular Biochemistry - October 25, 2009 Category: Biochemistry Authors: Webb LM, Arnholt AT, Venable ME Tags: Mol Cell Biochem Source Type: journals

Erratum to: Reduced endoplasmic reticulum luminal calcium links saturated fatty acid-mediated endoplasmic reticulum stress and cell death in liver cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19856078 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - October 24, 2009 Category: Biochemistry Authors: Wei Y, Wang D, Gentile CL, Pagliassotti MJ Tags: Mol Cell Biochem Source Type: journals

Distinct expression patterns of hedgehog ligands between cultured and primary colorectal cancers are associated with aberrant methylation of their promoters.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, ligand dependent activity of Hh pathway is inactive in cultured colon cancer cells correlating to ligands hypermethylation. In contrast, SHH overexpression, possible consequence of promoter hypomethylation, could play a role in the carcinogenesis of primary CRCs. PMID: 19856079 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - October 24, 2009 Category: Biochemistry Authors: Fu X, Deng H, Zhao L, Li J, Zhou Y, Zhang Y Tags: Mol Cell Biochem Source Type: journals

Phospholipase Cbeta(4) isozyme is expressed in human, rat, and murine heart left ventricles and in HL-1 cardiomyocytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, PLCbeta((1-4)) isozymes mRNA relative expression was studied by real-time PCR (RT-PCR) in human, rat, and murine left ventricle and the presence of PLCbeta(4) isozyme at the protein level was confirmed by Western blotting in all species studied. Confocal microscopy experiments carried out in HL-1 cardiomyocytes revealed a sarcoplasmic subcellular distribution of PLCbeta(4). Although there were unexpected significant interspecies differences in the PLCbeta((1-4)) mRNA expression, PLCbeta(4) mRNA was the main transcript expressed in all left ventricles studied. Thus, whereas in human and rat left ventricles PL...
Source: Molecular and Cellular Biochemistry - October 24, 2009 Category: Biochemistry Authors: Otaegui D, Querejeta R, Arrieta A, Lazkano A, Bidaurrazaga A, Arriandiaga JR, Aldazabal P, Garro MA Tags: Mol Cell Biochem Source Type: journals

The interaction between interferon-induced protein with tetratricopeptide repeats-1 and eukaryotic elongation factor-1A.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It has been shown previously that in mammalian cells, interferon-induced protein with tetratricopeptide repeats-1(IFIT1) is rapidly synthesized in response to viral infection, functions as an inhibitor of translation by binding to the eukaryotic initiation factor-3, and consequently assigns resistive activity against viral invasion to cells. It has also been reported that IFIT1 is rapidly produced in response to other cell stress agents with no direct relation to virus such as bacterial lipopolysaccharide and interleukin-1, but its function under these non-viral infection cell stress conditions has yet to be elucidated...
Source: Molecular and Cellular Biochemistry - October 24, 2009 Category: Biochemistry Authors: Li HT, Su YP, Cheng TM, Xu JM, Liao J, Chen JC, Ji CY, Ai GP, Wang JP Tags: Mol Cell Biochem Source Type: journals

Effect of adjuvants on immune response and protective immunity elicited by recombinant Hsp60 (GroEL) of Salmonella typhi against S. typhi infection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Heat shock proteins (Hsps) have been reported to be dominant antigens for the host immune response to various pathogens and thus, have great potential for use in vaccination. In the present study, we evaluated the immunogenicity and protective efficacy of GroEL of Salmonella enterica serovar Typhi against lethal infection by S. typhi Ty2 in mice with or without adjuvants. Anti GroEL-IgG titers were significantly higher in mice immunized with either GroEL-alone or in combination with alum/Complete Freund's adjuvant (CFA) as compared to the control. Analysis of antibody isotypes suggested predominance of Th2 type immune ...
Source: Molecular and Cellular Biochemistry - October 23, 2009 Category: Biochemistry Authors: Bansal A, Paliwal PK, Sagi SS, Sairam M Tags: Mol Cell Biochem Source Type: journals

Adipophilin affects the expression of TNF-alpha, MCP-1, and IL-6 in THP-1 macrophages.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we observe the effect of adipophilin on proinflammatory cytokine expression and secretion in THP-1 macrophages. SiRNA and adipophilin gene overexpression mediated by an pEGFP-C3 vector were used to observe the effect of adipophilin on proinflammatory cytokines in THP-1 macrophages in vitro. Realtime PCR and enzyme-linked immunosorbent assay (ELISA) were applied to detect the production of tumor necrosis factor alpha (TNF-alpha), monocyte chemoattractant protein 1 (MCP-1), and interleukin-6 (IL-6). It was found that acetylated low-density lipoprotein (AcLDL), pioglitazone [a peroxisome proliferator-activated ...
Source: Molecular and Cellular Biochemistry - October 23, 2009 Category: Biochemistry Authors: Chen FL, Yang ZH, Wang XC, Liu Y, Yang YH, Li LX, Liang WC, Zhou WB, Hu RM Tags: Mol Cell Biochem Source Type: journals

Downregulation of CREB-binding protein expression sensitizes endothelial cells to serum-deprived apoptosis: important role of nitric oxide.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we found that when CBP expression was silenced by RNA interference, ECs were more prone to apoptosis under serum deprivation, whereas the apoptosis was not significantly induced in the serum-containing condition. The increased apoptosis is paralleled by a reduction of NO, and the apoptosis was reversed by NO donors, suggesting an important role of NO. Furthermore, CBP silencing decreased NO production by downregulating the endothelial NO synthase (eNOS) expression in a dose-dependent manner. These results indicated that CBP silencing is associated with decreased eNOS expression and NO production, and therefo...
Source: Molecular and Cellular Biochemistry - October 23, 2009 Category: Biochemistry Authors: Chen J, Jiang H, Zhu LH, Wang L, Xu L Tags: Mol Cell Biochem Source Type: journals

Ectopic expression of hC/EBPs in breast tumor cells induces apoptosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CCAAT/enhancer binding proteins (C/EBPs) are a group of transcription factors which have been implicated in cellular proliferation, terminal differentiation, and apoptosis in a variety of tissues including mammary gland. Owing to its role in various cellular functions, inactivation of C/EBP proteins is central to the pathogenesis of many disorders. Recent reports suggest that expression as well as function of C/EBP proteins is deregulated in breast tumors. Although, role of C/EBPs in growth arrest in mammary tissues has been studied in much detail; their role in apoptosis is relatively less explored. In the present stu...
Source: Molecular and Cellular Biochemistry - October 23, 2009 Category: Biochemistry Authors: Pal P, Lochab S, Kanaujiya J, Sanyal S, Trivedi AK Tags: Mol Cell Biochem Source Type: journals

Histamine stimulates human lung fibroblast migration.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study demonstrated that histamine has the potential to stimulate human lung fibroblast migration, and thus may contribute to regulation of wound healing and the development of fibrotic disorders of the lung. PMID: 19851834 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - October 23, 2009 Category: Biochemistry Authors: Kohyama T, Yamauchi Y, Takizawa H, Kamitani S, Kawasaki S, Nagase T Tags: Mol Cell Biochem Source Type: journals

Opening of the mitoK(ATP) channel and decoupling of mitochondrial complex II and III contribute to the suppression of myocardial reperfusion hyperoxygenation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our results demonstrated that diazoxide suppressed the in vivo post-ischemic myocardial hyperoxygenation through opening the mitoK(ATP) channel and ubisemiquinone-dependent O(2) (-*) generation via inhibiting mitochondrial complex II-dependent respiration. PMID: 19851835 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - October 23, 2009 Category: Biochemistry Authors: Liu B, Zhu X, Chen CL, Hu K, Swartz HM, Chen YR, He G Tags: Mol Cell Biochem Source Type: journals

Expression and distribution of CYP3A genes, CYP2B22, and MDR1, MRP1, MRP2, LRP efflux transporters in brain of control and rifampicin-treated pigs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The in vivo effect of rifampicin, a potent ligand of PXR, on gene expression of CYP2B22, 3A22, 3A29, 3A46, CAR, PXR and MDR1, MRP1, MRP2, LRP transporters in liver and cortex, cerebellum, midbrain, hippocampus, meninges and brain capillaries of pig was investigated. Animals were treated i.p. with four daily doses of rifampicin (40 mg/kg). The basal mRNA expressions of the individual CYP3As, CYP2B22, CAR, and PXR in various brain regions, except meninges, were about or below 10% of the corresponding hepatic mRNA values, whereas the mRNAs of brain transporters were closer or comparable to those in liver. After pig treatm...
Source: Molecular and Cellular Biochemistry - October 21, 2009 Category: Biochemistry Authors: Nannelli A, Rossignolo F, Tolando R, Rossato P, Pellegatti M, Longo V, Giovanni Gervasi P Tags: Mol Cell Biochem Source Type: journals

Chitosan prevents oxidative stress-induced amyloid beta formation and cytotoxicity in NT2 neurons: involvement of transcription factors Nrf2 and NF-kappaB.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Increased oxidative stress is a widely accepted factor in the development and progression of Alzheimer's disease. Here, we introduce chitosan, an antioxidant oligosaccharide, as a protective agent against H(2)O(2)/FeSO(4)-induced cell death in the NT2 neural cell line. Chitosan not only protects the neurons against cell death, as measured by MTT and caspase-3 activity, but also decreases amyloid beta formation. NT2 neurons can be used to elucidate the relationship between oxidative stress and Abeta formation. We induced Abeta formation through oxidative stress in NT2 neurons and studied the effect of chitosan. We demon...
Source: Molecular and Cellular Biochemistry - October 20, 2009 Category: Biochemistry Authors: Khodagholi F, Eftekharzadeh B, Maghsoudi N, Rezaei PF Tags: Mol Cell Biochem Source Type: journals

Changes in the level of apoptosis-related proteins in Jurkat cells infected with HIV-1 versus HIV-2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human immunodeficiency virus (HIV) infection-induced apoptosis of infected CD4 T cells as well as uninfected (bystander) CD4 T cells and other types of cells is a major factor in the pathogenesis of AIDS. Clinically, HIV-2 patients have a higher CD4 cell count at the time of an AIDS diagnosis, and generally have longer survival after development of symptoms. The mortality after an AIDS diagnosis has been reported to be more influenced by CD4 cell count than HIV type. Previous studies have shown significant variations in cytopathic effects following in vitro infection with primary isolates of HIV-1 or HIV-2 subtypes; ho...
Source: Molecular and Cellular Biochemistry - October 19, 2009 Category: Biochemistry Authors: Wang X, Viswanath R, Zhao J, Tang S, Hewlett I Tags: Mol Cell Biochem Source Type: journals

MUC5AC production is downregulated in NCI-H292 lung cancer cells cultured on type-IV collagen.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated whether the signals from ECM regulate MUC5AC production in the human lung epithelial cell line NCI-H292. We found that MUC5AC production is downregulated in NCI-H292 cells cultured on type-IV collagen, a major component of ECM, but shows no obvious changes when cultured on type-I collagen or fibronectin. In contrast, MUC5AC production was upregulated on laminin and on reconstituted basement membrane (Matrigel), a complex of ECM components. Antibody-mediated inhibition of integrin beta1-subunit, a major receptor involved in the adherence of cells to type-IV collagen, upregulated the MUC5AC pro...
Source: Molecular and Cellular Biochemistry - October 15, 2009 Category: Biochemistry Authors: Iwashita J, Yamamoto T, Sasaki Y, Abe T Tags: Mol Cell Biochem Source Type: journals

GST profile expression study in some selected plants: in silico approach.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aims to comprehend the relationship of GST gene family and plant oxidative stress with respect to certain specific conserved motifs, which may help in future studies for screening of biomodulators involved in plant stress metabolism. PMID: 19838771 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - October 14, 2009 Category: Biochemistry Authors: Banerjee S, Goswami R Tags: Mol Cell Biochem Source Type: journals

Preface to the International Symposium on "Perspectives of cell signaling and molecular medicine"Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19830526 [PubMed - as supplied by publisher] (Source: Molecular and Cellular Biochemistry)
Source: Molecular and Cellular Biochemistry - October 14, 2009 Category: Biochemistry Authors: Sen PC Tags: Mol Cell Biochem Source Type: journals

Multifocal signal modulation therapy of cancer: ancient weapon, modern targets.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although safe in most cases, ancient treatments are ignored because neither their active components nor their molecular targets are well defined. This is not the case, however, with curcumin, a yellow-pigment substance and component of turmeric (Curcuma longa), which was identified more than a century ago. Recently, extensive research has addressed the chemotherapeutic potential of this relatively nontoxic-plant-derived polyphenol. Because most cancers are caused by deregulation of as many as 500 different genes, agents that target multiple gene products are needed for prevention and treatment of cancer. In this regard...
Source: Molecular and Cellular Biochemistry - October 13, 2009 Category: Biochemistry Authors: Das T, Sa G, Saha B, Das K Tags: Mol Cell Biochem Source Type: journals

Insulin resistance due to lipid-induced signaling defects could be prevented by mahanine.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It is well known that free fatty acids (FFAs) play a key role in implementing insulin resistance and type 2 diabetes. Resources of chemical compounds that intervene the derogatory effect of FFAs are indeed very limited. We have isolated mahanine, a carbazole alkaloid, from the leaves of Murraya koenegii that prevented palmitate-induced inhibition of insulin-stimulated phosphorylation of IRbeta, PI3K, PDK1, and Akt in L6 myotubes. This was also reflected in the palmitate-induced inhibition of insulin-stimulated [(3)H] 2-DOG uptake by L6 myotubes, where palmitate adverse effect was significantly blocked by mahanine. Prev...
Source: Molecular and Cellular Biochemistry - October 13, 2009 Category: Biochemistry Authors: Biswas A, Bhattacharya S, Dasgupta S, Kundu R, Roy SS, Pal BC, Bhattacharya S Tags: Mol Cell Biochem Source Type: journals

Genetic polymorphism of CYP3A5 in Indian chronic myeloid leukemia patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CYP3A5 is an important genetic contributor to inter-individual differences in CYP3A-dependent clinically important drugs of metabolism and also of various endogenous compounds and environmental contaminants. The CYP3A5*3 allele results in a truncated protein with loss of CYP3A5 expression and CYP3A5*6 is associated with lower CYP3A5 catalytic activity. The polymorphism analysis was performed by PCR-RFLP and some representative cases by direct sequencing. Our case control study involved 183 consecutive North Indian CML patients in chronic phase of disease and 208 geographically and racially matched healthy controls. PCR...
Source: Molecular and Cellular Biochemistry - October 12, 2009 Category: Biochemistry Authors: Bajpai P, Tripathi AK, Agrawal D Tags: Mol Cell Biochem Source Type: journals

Mechanism of noradrenaline-induced stimulation of Na-K ATPase activity in the rat brain: implications on REM sleep deprivation-induced increase in brain excitability.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rapid eye movement (REM) sleep is a unique phenomenon expressed in all higher forms of animals. Its quantity varies in different species and with ageing; it is also affected in several psycho-somatic disorders. Several lines of studies showed that after REM sleep loss, the levels of noradrenaline (NA) increase in the brain. The NA in the brain modulates neuronal Na-K ATPase activity, which helps maintaining the brain excitability status. The detailed mechanism of increase in NA level after REM sleep loss and the effect of NA on stimulation of Na-K ATPase in the neurons have been discussed. The findings have been review...
Source: Molecular and Cellular Biochemistry - October 12, 2009 Category: Biochemistry Authors: Mallick BN, Singh S, Singh A Tags: Mol Cell Biochem Source Type: journals

Telomerase activity and hepatic functions of rat embryonic liver progenitor cell in nanoscaffold-coated model bioreactor.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Presently, there is growing interest on telomerase activity in all cells (somatic cells, stem cells, cancerous cells and others) since this activity is associated with cellular changes such as proliferation, differentiation, immortalization, cell injury and ageing. Telomerase activity is absent in most of the somatic cells but present in over 90% of cancerous cells and other immortalized cell lines. In our present study, we cultured a rat embryonal liver progenitor cell line RLC-18 in a self-assembly nanostructured scaffold-coated bioreactor (NCB), collagen-coated plates (CCP) and uncoated plates (UP), and evaluated ch...
Source: Molecular and Cellular Biochemistry - October 8, 2009 Category: Biochemistry Authors: Giri S, Nieber K, Acikgöz A, Pavlica S, Keller M, Bader A Tags: Mol Cell Biochem Source Type: journals

Fibronectin-integrin mediated signaling in human cervical cancer cells (SiHa).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Interaction between cell surface integrin receptors and extracellular matrix (ECM) components plays an important role in cell survival, proliferation, and migration, including tumor development and invasion of tumor cells. Matrix metalloproteinases (MMPs) are a family of metalloproteinases capable of digesting ECM components and are important molecules for cell migration. Binding of ECM to integrins initiates cascades of cell signaling events modulating expression and activity of different MMPs. The aim of this study is to investigate fibronectin-integrin-mediated signaling and modulation of MMPs. Our findings indicate...
Source: Molecular and Cellular Biochemistry - October 8, 2009 Category: Biochemistry Authors: Maity G, Fahreen S, Banerji A, Roy Choudhury P, Sen T, Dutta A, Chatterjee A Tags: Mol Cell Biochem Source Type: journals

Evaluation of inhibitory activities of plant extracts on production of LPS-stimulated pro-inflammatory mediators in J774 murine macrophages.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Whole plant methanolic extracts of 14 traditionally used medicinal herbs were evaluated for their anti-inflammatory activity. Extracts of Grindelia robusta, Salix nigra, Arnica montana, and Quassia amara showed up to 4.5-fold inhibition of nitric oxide (NO) production in the J774 murine macrophage cells challenged with LPS without cytotoxicity. These four selected extracts significantly reduced the protein levels of inducible NO synthase (iNOS) and the cyclooxygenase-2 (COX-2) as observed by Western blot analysis. Culture supernatants from cells treated with these extracts indicated 3-5-fold reduction of tumor necrosis...
Source: Molecular and Cellular Biochemistry - October 7, 2009 Category: Biochemistry Authors: Verma N, Tripathi SK, Sahu D, Das HR, Das RH Tags: Mol Cell Biochem Source Type: journals

Enhanced death signaling in ozone-exposed ischemic-reperfused hearts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although numerous advancements made in the field of human health have resulted in reduced deaths due to cardiovascular diseases (CVD), many patients with cardiac disease show no established risk. Therefore, other unknown factors may be responsible for the pathophysiology of CVD. Out of 350,000 sudden cardiac deaths each year in the United States, 60,000 deaths have been related to air pollution, suggesting a detrimental role of environmental pollutants in the development of CVD. The present study tested our hypothesis that chronic ozone exposure enhances the sensitivity to ischemia-reperfusion (I/R) injury in isolated ...
Source: Molecular and Cellular Biochemistry - October 2, 2009 Category: Biochemistry Authors: Perepu RS, Garcia C, Dostal D, Sethi R Tags: Mol Cell Biochem Source Type: journals

Modulation of chromatin by MARs and MAR binding oncogenic transcription factor SMAR1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The orchestration of the events in the cell during the progression of the cell cycle is modulated by various phenomenon which are regulated by structural modules of the cell. The nucleus is a major hub for all these regulatory units which harbour the nuclear matrix, matrix proteins and chromatin. The histone modifications etch a complex code on the chromatin and the matrix proteins in consort with the histone code regulate the gene expression. SMAR1 is a matrix attachment region binding protein that interacts with chromatin modulators like HDAC1, Sin3A and causes chromatin condensation. SMAR1 modulates the chromatin at...
Source: Molecular and Cellular Biochemistry - October 2, 2009 Category: Biochemistry Authors: Nakka KK, Chattopadhyay S Tags: Mol Cell Biochem Source Type: journals

Regulation of tyrosine kinase activity during capacitation in goat sperm.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Protein tyrosine phosphorylation is a key event accompanying sperm capacitation. Although this signaling cascade generates an array of tyrosine-phosphorylated polypeptides, their molecular characterization is still limited. It is necessary to differentiate the localization of the tyrosine-phosphorylated proteins in spermatozoa to understand the link between the different phosphorylated proteins and the corresponding regulated sperm function. cAMP plays a pivotal role in the regulation of tyrosine phosphorylation. The intracellular cAMP levels were raised in goat spermatozoa by the addition of the phosphodiesterase inhi...
Source: Molecular and Cellular Biochemistry - October 2, 2009 Category: Biochemistry Authors: Chatterjee M, Nandi P, Ghosh S, Sen PC Tags: Mol Cell Biochem Source Type: journals

TNF-alpha modulates iNOS expression in an experimental rat model of indomethacin-induced jejunoileitis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Multiple mucosal immune factors, such as TNF-alpha and IL-1beta, are thought to be key mediators involved in inflammatory bowel disease. We evaluated the role of the pro-inflammatory cytokine TNF-alpha on nitric oxide synthase (NOS) expression in indomethacin-induced jejunoileitis in rats. Jejunoileitis was induced in rats with subcutaneous injections of indomethacin (7.5 mg/kg) 24 h apart for two consecutive days, and animals were randomized into four groups. Group 1 received only indomethacin. Group 2 was treated with a daily dose of phosphodiesterase (PDE) inhibitor (theophylline or pentoxifylline) by oral gavage fo...
Source: Molecular and Cellular Biochemistry - October 2, 2009 Category: Biochemistry Authors: Nandi J, Saud B, Zinkievich JM, Yang ZJ, Levine RA Tags: Mol Cell Biochem Source Type: journals

Activation of RhoA and FAK induces ERK-mediated osteopontin expression in mechanical force-subjected periodontal ligament fibroblasts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The precise mechanism by which Rho kinase translates the mechanical signals into OPN up-regulation in force-exposed fibroblasts has not been elucidated. Human periodontal ligament fibroblasts (hPLFs) were exposed to mechanical force by centrifuging the culture plates at a magnitude of 50 g/cm(2) for 60 min. At various times of the force application, they were processed for analyzing cell viability, trypan blue exclusion, and OPN expression at protein and RNA levels. Cellular mechanism(s) of the force-induced OPN up-regulation was also examined using various kinase inhibitors or antisense oligonucleotides specific to me...
Source: Molecular and Cellular Biochemistry - October 1, 2009 Category: Biochemistry Authors: Hong SY, Jeon YM, Lee HJ, Kim JG, Baek JA, Lee JC Tags: Mol Cell Biochem Source Type: journals

Antihyperlipidemic effect of bis-1,7-(2-hydroxyphenyl)-hepta-1,6-diene-3,5-dione, a curcumin analog, on nicotine and streptozotocin treated rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Diabetes and smoking have been considered as major health problems individually and their seriousness related to health hazard has been well reported. Data regarding the possible contribution of cigarette smoking to the development of diabetes are scarce and inconclusive. The aim was to investigate the effect of nicotine on diabetes and to analyze the effect of bis demethoxy curcumin analog (BDMCA) in streptozotocin (STZ) and nicotine-induced toxicity. The tissue lipids were extracted according to the method of Folch et al. Plasma and tissue cholesterol was estimated by the method of Allain et al. using reagent kit. Tr...
Source: Molecular and Cellular Biochemistry - September 29, 2009 Category: Biochemistry Authors: Reddy BV, Sivagama Sundari J, Balamurugan E, Menon VP Tags: Mol Cell Biochem Source Type: journals

Homocysteine modulates the proteolytic potential of human arterial smooth muscle cells through a reactive oxygen species dependant mechanism.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pathological levels of homocysteine induce a dramatic degradation of arterial elastic structures. This severe metalloproteinase-dependant elastolysis affects elastic structures all over the media suggesting that smooth muscle cells (SMC) may participate to this process induced by homocysteine. Therefore, we investigated the effect of physiological (10 muM) and pathological (50, 100, and 500 muM) concentrations of homocysteine on the metalloproteinase-dependant proteolytic potential of human arterial SMC in culture. Pathological levels of homocysteine increased concomitantly the secretion of latent MMP-2 and TIMP-2 whil...
Source: Molecular and Cellular Biochemistry - September 28, 2009 Category: Biochemistry Authors: Ke XD, Foucault-Bertaud A, Genovesio C, Dignat-George F, Lamy E, Charpiot P Tags: Mol Cell Biochem Source Type: journals