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Synchronous culture of Plasmodium falciparum at high parasitemia levelsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
et, Amalia Diez & José M Bautista This protocol describes a method for preparing cultures of Plasmodium falciparum synchronized at any intraerythrocytic stage. Using this method, around 60% parasitized cells may be obtained. On the basis of Trager and Jensen's original continuous culture method, our approach relies on the use of (Source: Nature Protocols)
Source: Nature Protocols - November 19, 2009 Category: Biomedical Science Authors: Azar RadfarDarío MéndezCarlos MonerizMaría LinaresPatricia Marín-GarcíaAntonio PuyetAmalia DiezJosé M Bautista Tags: Protocol Source Type: journals

Global identification of protein kinase substrates by protein microarray analysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
l Snyder Herein, we describe a protocol for the global identification of in vitro substrates targeted by protein kinases using protein microarray technology. Large numbers of fusion proteins tagged at their carboxy-termini are purified in 96-well format and spotted in duplicate onto amino-silane-coated slides in a (Source: Nature Protocols)
Source: Nature Protocols - November 19, 2009 Category: Biomedical Science Authors: Janine MokHogune ImMichael Snyder Tags: Protocol Source Type: journals

Determination of gene expression patterns using in situ hybridization to Drosophila testesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a whole-mount RNA in situ hybridization (ISH) method optimized for detection of the cellular and subcellular distributions of specific mRNA within Drosophila testes and male genital tract. Digoxygenin (dig)-labeled antisense RNA probes are in vitro transcribed from a template synthesized (Source: Nature Protocols)
Source: Nature Protocols - November 19, 2009 Category: Biomedical Science Authors: Ceri A MorrisElizabeth BensonHelen White-Cooper Tags: Protocol Source Type: journals

Protocols to detect senescence-associated beta-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Protocols to detect senescence-associated beta-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivo Nature Protocols 4, 1798 (2009). doi:10.1038/nprot.2009.191 Authors: Florence Debacq-Chainiaux, Jorge D Erusalimsky, Judith Campisi & Olivier Toussaint Normal cells can permanently lose the ability to proliferate when challenged by potentially oncogenic stress, a process termed cellular senescence. Senescence-associated beta-galactosidase (SA-βgal) activity, detectable at pH 6.0, permits the identification of senescent cells in culture and mammalian tissues. Here we describe...
Source: Nature Protocols - November 19, 2009 Category: Biomedical Science Authors: Florence Debacq-ChainiauxJorge D ErusalimskyJudith CampisiOlivier Toussaint Tags: Protocol Source Type: journals

Simultaneous analysis of reactive oxygen species and reduced glutathione content in living cells by polychromatic flow cytometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
mp; Marcello Pinti Reactive oxygen species (ROS) are continuously produced in the cell as a consequence of aerobic metabolism, and are controlled by several antioxidant mechanisms. An accurate measurement of ROS is essential to evaluate the redox status of the cell, or the effects of molecules with the (Source: Nature Protocols)
Source: Nature Protocols - November 12, 2009 Category: Biomedical Science Authors: Andrea CossarizzaRoberta FerraresiLeonarda TroianoErika RoatLara GibelliniLinda BertoncelliMilena NasiMarcello Pinti Tags: Protocol Source Type: journals

Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic codeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a protocol for the heterologous expression of selectively tyrosine-sulfated proteins in Escherichia coli through the use of an expanded genetic (Source: Nature Protocols)
Source: Nature Protocols - November 12, 2009 Category: Biomedical Science Authors: Chang C LiuSusan E CellittiBernhard H GeierstangerPeter G Schultz Tags: Protocol Source Type: journals

High-throughput haplotype determination over long distances by haplotype fusion PCR and ligation haplotypingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hurles When combined with haplotype fusion PCR (HF-PCR), ligation haplotyping is a robust, high-throughput method for empirical determination of haplotypes, which can be applied to assaying both sequence and structural variation over long distances. Unlike alternative approaches to haplotype determination, such as allele-specific PCR and long (Source: Nature Protocols)
Source: Nature Protocols - November 12, 2009 Category: Biomedical Science Authors: Daniel J TurnerMatthew E Hurles Tags: Protocol Source Type: journals

High-yield cell-free protein production from P-gelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
o Um & Dan Luo Cell-free systems represent a promising approach to quickly and easily produce preparative amounts of proteins. However, it is still challenging to obtain high volumetric yields (>mg ml−1) of proteins from the present cell-free systems. This protocol presents a cell-free protein synthesis method using (Source: Nature Protocols)
Source: Nature Protocols - November 12, 2009 Category: Biomedical Science Authors: Nokyoung ParkJason S KahnEdward J RiceMark R HartmanHisakage FunabashiJianfeng XuSoong Ho UmDan Luo Tags: Protocol Source Type: journals

Measuring bone blood supply in mice using fluorescent microspheresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nature Protocols 4, 1779 (2009). doi:10.1038/nprot.2009.190 Author: Maria A Serrat Fluorescent microspheres are commonly used to assess bone blood supply in large animals, but the technique is not widely used in smaller mammals, as traditional methods such as reference blood sampling, ventilation and catheterization are not easily applied. This protocol describes a viable alternative for (Source: Nature Protocols)
Source: Nature Protocols - November 5, 2009 Category: Biomedical Science Authors: Maria A Serrat Tags: Protocol Source Type: journals

Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ian P Limbourg Blood vessel growth in adult organisms involves the following two fundamental processes: angiogenesis, the proliferation and extension of capillary networks; and arteriogenesis, the growth of functional arteries. We provide a protocol for the evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb (Source: Nature Protocols)
Source: Nature Protocols - November 5, 2009 Category: Biomedical Science Authors: Anne LimbourgThomas KorffL Christian NappWolfgang SchaperHelmut DrexlerFlorian P Limbourg Tags: Protocol Source Type: journals

Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarraysEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
gel P Carter Array painting is a technique that uses microarray technology to rapidly map chromosome translocation breakpoints. Previous methods to map translocation breakpoints have used fluorescence in situ hybridization (FISH) and have consequently been labor-intensive, time-consuming and restricted to the low breakpoint resolution imposed by the (Source: Nature Protocols)
Source: Nature Protocols - November 5, 2009 Category: Biomedical Science Authors: Susan M GribbleBee Ling NgElena PrigmoreTomas FitzgeraldNigel P Carter Tags: Protocol Source Type: journals

Assessment of pyrogenic contaminations with validated human whole-blood assayEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present an internationally validated protocol for the evaluation of pyrogenic contaminations using human whole blood. In the in vitro pyrogen test (IPT) the sample is incubated with fresh or cryopreserved human whole blood, and the proinflammatory cytokine interleukin-1β (IL-1β) is detected by enzyme-linked (Source: Nature Protocols)
Source: Nature Protocols - November 5, 2009 Category: Biomedical Science Authors: Mardas DaneshianSonja von AulockThomas Hartung Tags: Protocol Source Type: journals

Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
L Chan The sunflower belongs to the Compositae family and is an economically important crop because of the quality of its oil. Unfortunately, molecular analyses are limited due to the lack of genomic information, mutant libraries and efficient and rapid transformation protocols. In a wide variety (Source: Nature Protocols)
Source: Nature Protocols - October 29, 2009 Category: Biomedical Science Authors: Pablo A ManavellaRaquel L Chan Tags: Protocol Source Type: journals

Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structuresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
yoon Han The anisotropic nanofluidic-filter (nanofilter) array (ANA) is a unique molecular-sieving structure for separating biomolecules. In this protocol we describe the fabrication of planar and vertical ANA chips and how to perform continuous-flow bioseparation using them. This protocol is most useful for bioengineers who are interested (Source: Nature Protocols)
Source: Nature Protocols - October 29, 2009 Category: Biomedical Science Authors: Jianping FuPan MaoJongyoon Han Tags: Protocol Source Type: journals

Generation of orthotopic and heterotopic human pancreatic cancer xenografts in immunodeficient miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Gary E Gallick For decades, xenografts using well-established human tumor cell lines have been the most commonly used models to study human cancers in mice. More recently, human tumors implanted directly into immunodeficient mice have become increasingly popular as evidence accrues that they more accurately recapitulate features of (Source: Nature Protocols)
Source: Nature Protocols - October 29, 2009 Category: Biomedical Science Authors: Michael P KimDouglas B EvansHuamin WangJames L AbbruzzeseJason B FlemingGary E Gallick Tags: Protocol Source Type: journals

A reporter gene imaging system for monitoring microRNA biogenesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
oonhag Kim MicroRNAs (miRNAs), non-coding RNA molecules, have emerged as a part of key gene regulation, participating in a variety of biological processes such as cell development. Current research methods, including northern blot and real-time PCR analysis, have been used to quantify miRNA expression. Major disadvantages of (Source: Nature Protocols)
Source: Nature Protocols - October 29, 2009 Category: Biomedical Science Authors: Hae Young KoDo Won HwangDong Soo LeeSoonhag Kim Tags: Protocol Source Type: journals

Induction of asymmetrical cell division to analyze spindle-dependent organelle partitioning using correlative microscopy techniquesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
eemann This protocol describes an assay for the induction of asymmetrical cell division where the entire spindle is segregated into only one of the daughter cells. The procedure consists of four stages: (i) generation of asymmetrical monoasters by arresting cells in early mitosis with a kinesin (Source: Nature Protocols)
Source: Nature Protocols - October 21, 2009 Category: Biomedical Science Authors: Jen-Hsuan WeiJoachim Seemann Tags: Protocol Source Type: journals

A protocol for islet isolation from mouse pancreasEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a protocol for islet isolation from mouse pancreas. Three major manipulations are employed in the islet isolation procedure: in situ pancreas perfusion (Source: Nature Protocols)
Source: Nature Protocols - October 21, 2009 Category: Biomedical Science Authors: Dong-Sheng LiYa-Hong YuanHan-Jun TuQing-Le LiangLong-Jun Dai Tags: Protocol Source Type: journals

Isotope labeling methods for studies of excited protein states by relaxation dispersion NMR spectroscopyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
p; Lewis E Kay The utility of nuclear magnetic resonance (NMR) spectroscopy as a tool for the study of biomolecular structure and dynamics has benefited from the development of facile labeling methods that incorporate NMR active probes at key positions in the molecule. Here we describe a protocol for (Source: Nature Protocols)
Source: Nature Protocols - October 21, 2009 Category: Biomedical Science Authors: Patrik LundströmPramodh VallurupalliD Flemming HansenLewis E Kay Tags: Protocol Source Type: journals

Investigation of mRNA quadruplex formation in Escherichia coliEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
g S Hartig The protocol presented here allows for the investigation of the formation of unusual nucleic acid structures in the 5′-untranslated region (UTR) of bacteria by correlating gene expression levels to the in vitro stability of the respective structure. In particular, we describe the introduction of (Source: Nature Protocols)
Source: Nature Protocols - October 21, 2009 Category: Biomedical Science Authors: Markus WielandJörg S Hartig Tags: Article Source Type: journals

Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
atsuda Small GTPases act as molecular switches that regulate a variety of cellular functions, such as proliferation, cell movement and vesicle trafficking. Genetically encoded biosensors based on the principle of fluorescence resonance energy transfer (FRET) can visualize a spatio-temporal activity of small GTPases in living cells, (Source: Nature Protocols)
Source: Nature Protocols - October 14, 2009 Category: Biomedical Science Authors: Kazuhiro AokiMichiyuki Matsuda Tags: Protocol Source Type: journals

Differentiation of human oligodendrocytes from pluripotent stem cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
un Zhang We have developed a four-part protocol to differentiate human embryonic stem cells (hESCs) to oligodendrocyte progenitor cells (OPCs) according to developmental principles. In the first 2 weeks, hESCs are induced to differentiate into neuroepithelial cells, which form neural tube–like rosettes. In the following 10 d, (Source: Nature Protocols)
Source: Nature Protocols - October 14, 2009 Category: Biomedical Science Authors: Bao-Yang HuZhong-Wei DuSu-Chun Zhang Tags: Protocol Source Type: journals

The morphological and molecular features of the epithelial-to-mesenchymal transitionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
o Portillo & Amparo Cano Here we describe several methods for the characterization of epithelial–mesenchymal transition (EMT) at the cellular, molecular and behavioral level. This protocol describes both in vitro and in vivo approaches designed to analyze different features that when taken together permit the characterization of cells (Source: Nature Protocols)
Source: Nature Protocols - October 14, 2009 Category: Biomedical Science Authors: Gema Moreno-BuenoHéctor PeinadoPatricia MolinaDavid OlmedaEva CubilloVanesa SantosJosé PalaciosFrancisco PortilloAmparo Cano Tags: Protocol Source Type: journals

Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Paolo Pinton Many cellular processes require the proper cooperation between mitochondria and the endoplasmic reticulum (ER). Several recent works show that their functional interactions rely on dynamic structural contacts between both organelles. Such contacts, called mitochondria-associated membranes (MAMs), are crucial for the synthesis and intracellular transport of (Source: Nature Protocols)
Source: Nature Protocols - October 7, 2009 Category: Biomedical Science Authors: Mariusz R WieckowskiCarlotta GiorgiMagdalena LebiedzinskaJerzy DuszynskiPaolo Pinton Tags: Protocol Source Type: journals

Housing conditions and stimulus females: a robust social discrimination task for studying male rodent social recognitionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
tt Young Social recognition (SR) enables rodents to distinguish between familiar and novel conspecifics, largely through individual odor cues. SR tasks utilize the tendency for a male to sniff and interact with a novel individual more than a familiar individual. Many paradigms have been used to study (Source: Nature Protocols)
Source: Nature Protocols - October 7, 2009 Category: Biomedical Science Authors: Abbe H MacbethJennifer Stepp EddsW Scott Young Tags: Protocol Source Type: journals

Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
t & Lois E H Smith The mouse model of oxygen-induced retinopathy (OIR) has been widely used in studies related to retinopathy of prematurity, proliferative diabetic retinopathy and in studies evaluating the efficacy of antiangiogenic compounds. In this model, 7-d-old (P7) mouse pups with nursing mothers are subjected to hyperoxia (75% (Source: Nature Protocols)
Source: Nature Protocols - October 7, 2009 Category: Biomedical Science Authors: Kip M ConnorNathan M KrahRoberta J DennisonChristopher M AdermanJing ChenKaren I GuerinPrzemyslaw SapiehaAndreas StahlKeirnan L WillettLois E H Smith Tags: Protocol Source Type: journals

The adhesive removal test: a sensitive method to assess sensorimotor deficits in miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bard & Thomas Freret Long-term functional deficits after a brain injury are difficult to assess in the mouse. If no deficit is observed, researchers could conclude either that the animal has fully recovered or that the tests they used were not appropriate or sensitive enough to the modality of (Source: Nature Protocols)
Source: Nature Protocols - September 30, 2009 Category: Biomedical Science Authors: Valentine BouetMichel BoulouardJérôme ToutainDidier DivouxMyriam BernaudinPascale Schumann-BardThomas Freret Tags: Protocol Source Type: journals

In vivo dendritic calcium imaging with a fiberoptic periscope systemEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Larkum Dendritic recordings in freely moving animals present great challenges using the current approaches. Here we present in detail a microendoscopic technique (the 'periscope' method) for measuring intracellular calcium activity directly from the apical dendrites of L5 pyramidal neurons from the pia down to depths of (Source: Nature Protocols)
Source: Nature Protocols - September 30, 2009 Category: Biomedical Science Authors: Masanori MurayamaMatthew E Larkum Tags: Protocol Source Type: journals

Network visualization and analysis of gene expression data using BioLayout Express3DEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
C Freeman Network analysis has an increasing role in our effort to understand the complexity of biological systems. This is because of our ability to generate large data sets, where the interaction or distance between biological components can be either measured experimentally or calculated. Here we describe (Source: Nature Protocols)
Source: Nature Protocols - September 30, 2009 Category: Biomedical Science Authors: Athanasios TheocharidisStjin van DongenAnton J EnrightTom C Freeman Tags: Protocol Source Type: journals

Mesoscopic hydrogel molding to control the 3D geometry of bioartificial muscle tissuesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
nad Bursac This protocol describes a cell/hydrogel molding method for precise and reproducible biomimetic fabrication of three-dimensional (3D) muscle tissue architectures in vitro. Using a high aspect ratio soft lithography technique, we fabricate polydimethylsiloxane (PDMS) molds containing arrays of mesoscopic posts with defined size, elongation and (Source: Nature Protocols)
Source: Nature Protocols - September 23, 2009 Category: Biomedical Science Authors: Weining BianBrian LiauNima BadieNenad Bursac Tags: Protocol Source Type: journals

Separation and detection of large phosphoproteins using Phos-tag SDS-PAGEEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ru Koike We provide a standard phosphate-affinity SDS-PAGE (Mn2+–Phos-tag SDS-PAGE) protocol, in which Phos-tag is used to analyze large phosphoproteins with molecular masses of more than 200 kDa. A previous protocol required a long electrophoresis time of 12 h for separation of phosphoisotypes of large (Source: Nature Protocols)
Source: Nature Protocols - September 23, 2009 Category: Biomedical Science Authors: Eiji KinoshitaEmiko Kinoshita-KikutaTohru Koike Tags: Protocol Source Type: journals

Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screeningEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Perrimon We provide a detailed protocol for the mass culturing of primary cells dissociated from Drosophila embryos. The advantage of this protocol over others is that we have optimized it for a robust large-scale performance that is suitable for screening. More importantly, we further present (Source: Nature Protocols)
Source: Nature Protocols - September 23, 2009 Category: Biomedical Science Authors: Jianwu BaiKatharine J SeppNorbert Perrimon Tags: Protocol Source Type: journals

Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arraysEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Keith Joung Engineered zinc-finger nucleases (ZFNs) form the basis of a broadly applicable method for targeted, efficient modification of eukaryotic genomes. In recent work, we described OPEN (oligomerized pool engineering), an 'open-source,' combinatorial selection-based method for engineering zinc-finger arrays that function well as ZFNs. We have also (Source: Nature Protocols)
Source: Nature Protocols - September 16, 2009 Category: Biomedical Science Authors: Morgan L MaederStacey Thibodeau-BegannyJeffry D SanderDaniel F VoytasJ Keith Joung Tags: Protocol Source Type: journals

Generation of induced pluripotent stem cells from neural stem cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ans R Schöler The generation of induced pluripotent stem (iPS) cells from mouse and human somatic cells by expression of defined transcription factors (Oct4, Sox2, c-Myc, Klf4, Nanog and Lin28) is a powerful tool for conducting basic research and investigating the potential (Source: Nature Protocols)
Source: Nature Protocols - September 16, 2009 Category: Biomedical Science Authors: Jeong Beom KimHolm ZaehresMarcos J Araúzo-BravoHans R Schöler Tags: Protocol Source Type: journals

Generation of cortical neurons from mouse embryonic stem cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
erre Vanderhaeghen Embryonic stem cells (ESCs) constitute a tool of great potential in neurobiology, enabling the directed differentiation of specific neural cell types. We have shown recently that neurons of the cerebral cortex can be generated from mouse ESCs cultured in a chemically defined medium that contains (Source: Nature Protocols)
Source: Nature Protocols - September 16, 2009 Category: Biomedical Science Authors: Nicolas GaspardTristan BouschetAdèle HerpoelGilles NaeijeJelle van den AmeelePierre Vanderhaeghen Tags: Protocol Source Type: journals

Attachment of stem cells to scaffold particles for intra-cerebral transplantationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
p; Michel Modo Cell-replacement therapy and tissue regeneration using stem cells are of great interest to recover histological damage caused by neuro-degenerative disease or traumatic insults to the brain. To date, the main intra-cerebral delivery for these cells has been as a suspension in media through a thin (Source: Nature Protocols)
Source: Nature Protocols - September 16, 2009 Category: Biomedical Science Authors: Ellen BibleDavid Y S ChauMorgan R AlexanderJack PriceKevin M ShakesheffMichel Modo Tags: Protocol Source Type: journals

Highly efficient subcloning of rodent malaria parasites by injection of single merosomes or detached cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
r T Heussler This protocol describes a method for obtaining rodent Plasmodium parasite clones with high efficiency, which takes advantage of the normal course of Plasmodium in vitro exoerythrocytic development. At the completion of development, detached cells/merosomes form, which contain hundreds to thousands of merozoites. As (Source: Nature Protocols)
Source: Nature Protocols - September 9, 2009 Category: Biomedical Science Authors: Rebecca R StanwayStefanie GraeweAnnika RennenbergSusanne HelmVolker T Heussler Tags: Protocol Source Type: journals

Chemical genetic screening in the zebrafish embryoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
nard Zon Chemical genetic screening can be described as a discovery approach in which chemicals are assayed for their effects on a defined biological system. The zebrafish, Danio rerio, is a well-characterized and genetically tractable vertebrate model organism that produces large numbers of rapidly developing embryos (Source: Nature Protocols)
Source: Nature Protocols - September 9, 2009 Category: Biomedical Science Authors: Charles K KaufmanRichard M WhiteLeonard Zon Tags: Protocol Source Type: journals

Time-resolved RNA SHAPE chemistry: quantitative RNA structure analysis in one-second snapshots and at single-nucleotide resolutionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Weeks RNA selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) chemistry exploits the discovery that conformationally dynamic nucleotides preferentially adopt configurations that facilitate reaction between the 2′-OH group and a hydroxyl-selective electrophile, such as benzoyl cyanide (BzCN), to form a 2′-O-adduct. BzCN is ideally (Source: Nature Protocols)
Source: Nature Protocols - September 9, 2009 Category: Biomedical Science Authors: Stefanie A MortimerKevin M Weeks Tags: Protocol Source Type: journals

Quantitative imaging of collective cell migration during Drosophila gastrulation: multiphoton microscopy and computational analysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
athopoulos This protocol describes imaging and computational tools to collect and analyze live imaging data of embryonic cell migration. Our five-step protocol requires a few weeks to move through embryo preparation and four-dimensional (4D) live imaging using multi-photon microscopy, to 3D cell tracking using image processing, (Source: Nature Protocols)
Source: Nature Protocols - September 9, 2009 Category: Biomedical Science Authors: Willy SupattoAmy McMahonScott E FraserAngelike Stathopoulos Tags: Protocol Source Type: journals

Quantitative imaging of collective cell migration during Drosophila gastrulation: multiphoton microscopy and computational analysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
athopoulos This protocol describes imaging and computational tools to collect and analyze live imaging data of embryonic cell migration. Our five-step protocol requires a few weeks to move through embryo preparation and four-dimensional (4D) live imaging using multi-photon microscopy, to 3D cell tracking using image processing, (Source: Nature Protocols)
Source: Nature Protocols - September 9, 2009 Category: Biomedical Science Authors: Scott E FraserAmy McMahonWilly SupattoAngelike Stathopoulos Tags: Protocol Source Type: journals

Expression, purification and use of recombinant annexin V for the detection of apoptotic cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
J Martin Apoptosis is a mode of programmed cell death that is widely used to eliminate cells during development, tissue homeostasis, infection or in response to injury. Alterations to the plasma membranes of apoptotic cells trigger recognition and engulfment of such cells by phagocytes. Measurement of plasma (Source: Nature Protocols)
Source: Nature Protocols - September 2, 2009 Category: Biomedical Science Authors: Susan E LogueMohamed ElgendySeamus J Martin Tags: Protocol Source Type: journals

Preparation of carbon nanotube bioconjugates for biomedical applicationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
ongjie Dai Biomedical applications of carbon nanotubes have attracted much attention in recent years. Here, we summarize our previously developed protocols for functionalization and bioconjugation of single-walled carbon nanotubes (SWNTs) for various biomedical applications including biological imaging; using nanotubes as Raman, photoluminescence and photoacoustic labels; sensing using (Source: Nature Protocols)
Source: Nature Protocols - September 2, 2009 Category: Biomedical Science Authors: Zhuang LiuScott M TabakmanZhuo ChenHongjie Dai Tags: Protocol Source Type: journals