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Psychological well-being in advanced NSCLC patients in Serbia: impact of sociodemographic and clinical factors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Patients with advanced non-small cell lung cancer (NSCLC) usually undergo toxic treatment (chemotherapy and/or radiotherapy). They can experience devastating effects of illness and therapies on their psychological and emotional well-being. On the other hand, untreated psychological distress is associated with reduced quality of life and inadequate palliation of physical symptoms. In order to estimate frequency of anxiety and depressive symptoms and influence of demographic, socioeconomic and clinical factors on psychological well-being, we performed this cross-sectional study in group of 100 patients with ad...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Maric DM, Jovanovic DM, Golubicic IV, Nagorni-Obradovic LJ, Stojsic JM, Pekmezovic TD Tags: Neoplasma Source Type: journals

Thalidomide and bortezomib overcome the prognostic significance of proliferative index in multiple myeloma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We analyzed proliferative index of myeloma plasmocytes (PC-PI) in acohort of 217 patients with multiple myeloma (MM) treated with conventional chemotherapy and biological agents, thalidomide and bortezomib. In the whole group was adifference between overall survival (OS) favoring patients with PC-PI ven after 40 months (median overall survival 25 vs 10months, p= 0.015), whereas in the group treated with thalidomide and bortezomib was no difference, with medians over 39 months. Even patients with low PC-PI profited from the treatment with novel drugs. Presented results suggest that the treatment of MM with novel agents ...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Minarik J, Scudla V, Bacovsky J, Zemanova M, Pika T, Ordeltova M, Langova K Tags: Neoplasma Source Type: journals

CYP1B1 gene polymorphism modifies pancreatic cancer risk but not survival.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pancreatic cancer represents one of the biggest problems of current oncology. The risk factors of pancreatic cancer development, as well as factors affecting survival are poorly understood. Since biotransformation enzymes modify detoxification of carcinogens, we supposed, that arelationship between their polymorphism and the risk of pancreatic cancer development and eventually its clinical outcome may exist. Associations of so far not studied cytochrome P450 1B1 (CYP1B1) polymorphisms with pancreatic cancer risk were investigated by case-control study. Atotal of 754 participants were recruited during study p...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Vrana D, Novotny J, Holcatova I, Hlavata I, Soucek P Tags: Neoplasma Source Type: journals

Comparative study of the immunohistochemical expression of metalloproteinases 2, 7and 9between clearly invasive carcinomas and "in situ" trophoblast invasion.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, although there seems to be adirect link between cancer invasion and MMP9 immunohistochemical expression, the role of MMP2 and MMP7 appears to be more complicated underlining the complexity of the mechanisms involved in cancer spreading. Keywords: PMID: 19895168 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Ioannidis I, Dimo B, Karameris A, Vilaras G, Gakiopoulou H, Patsouris E, Lazaris AC Tags: Neoplasma Source Type: journals

Pretreatment hepatocyte growth factor and thrombospondin-1 levels predict response to high-dose chemotherapy for multiple myeloma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Our aim was to establish whether the pretreatment levels of angiogenesis activators and inhibitors can be used to predict clinical responses to treatment that included high-dose chemotherapy with peripheral stem cell support. We analyzed samples and treatment outcomes of 96 patients with MM enrolled in the CMG 2002 randomized clinical trial and treated with induction chemotherapy and high-dose chemotherapy with stem cell support. Concentrations of vascular endothelial growth factor (VEGF), hepatocytar growth factor (HGF), basic fibroblastic growth factor (bFGF), thrombospondin-1 (TSP-1), endostatin, and angi...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Pour L, Svachova H, Adam Z, Mikulkova Z, Buresova L, Kovarova L, Buchler T, Penka M, Vorlicek J, Hajek R Tags: Neoplasma Source Type: journals

Hypermethylation of the GSTP1 promoter region in breast cancer is associated with prognostic clinicopathological parameters.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Breast cancer is one of the most common cancer affecting women and the recent research is focused on identifying new genetic and epigenetic prognostic and predictive factors. Glutathione S-transferase P1 (GSTP1) is abiotransformation enzyme expressed in normal breast epithelial cells which can be epigenetically inactivated in breast cancer. We have shown, that application of nested two-stage methylation-specific PCR (MSP) is asuitable method for analysis of epigenetically silenced GSTP1 in formalin-fixed paraffin-embedded (FFPE) tissues from breast cancer patients. Of 45 breast tumors, 11 (24, 4%) were found to have me...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Lasabova Z, Tilandyova P, Kajo K, Zubor P, Burjanivova T, Danko J, Plank L Tags: Neoplasma Source Type: journals

Immunoelectron microscopy in mycosis fungoides and benign dermatoses. Expression of CD3, CD4 and CD7 receptors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mycosis fungoides is an epidermotropic cutaneous T-cell lymphoma (CTCL).Specimens for presented study were taken from sixteen patients with MF confirmed by immunohistochemical methods and PCR and from nine patients with benign dermatoses. To demonstrate CD3, CD4 and CD7 antigens immunogold method was used. We saw morphological differences between lymphocytes from MF and benign dermatoses. In MF, CD3 and CD4 were present rather in form of clusters placed on the surface of cell. On the contrary -CD3 to CD7 distribution analysis showed that these antigens were present rather individually, however there were seen clusters ...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Grzanka AA, Placek W, Grzanka A, Sokolowska-Wojdylo M, Zuryn A Tags: Neoplasma Source Type: journals

A twisted kiss: in vitro and in vivo evidence of genetic variation and suppressed transcription of the metastasis-suppressor gene KiSS1 in early breast cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, altered nucleotide sequence and repression of transcription are two potential mechanisms of suppression of the anti-metastatic effects of KiSS1 in early breast cancer: Confirmation in larger cohorts and study of functional effects of the 242C>G exon IVa mutation are warranted. Keywords: KiSS1, metastasis-suppressor gene, breast cancer, mutation, transcription. PMID: 19895172 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Pentheroudakis G, Kostadima L, Dova L, Georgiou I, Tzavaras T, Vartholomatos G, Wirtz RM, Fountzilas G, Malamou-Mitsi V, Pavlidis N Tags: Neoplasma Source Type: journals

Receptor for advanced glycation end products (RAGE) soluble form (sRAGE): anew biomarker for lung cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Receptor for advanced glycation end products (RAGE) may be involved in the pathogenesis of cancer progression. Pathological effects mediated via RAGE are physiologically inhibited by soluble RAGE (sRAGE). The aim of this study was to identify sRAGE and RAGE expression profile in lung cancer patients. An ELISA method was used to quantify serum sRAGE in 45 individuals. Additionally, surgical specimens of 28 lung cancer patients were also included for RAGE expression by immunohistochemistry. Serum sRAGE was significantly decreased in lung cancer patients compared with controls (vs. healthy donors, P=0.034; vs. pulmonary t...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Jing R, Cui M, Wang J, Wang H Tags: Neoplasma Source Type: journals

Expression and its clinical significance of heat shock protein gp96 in human osteosarcoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of the study was to observe the expression of heat shock protein gp96 (HSPgp96) and explore its clinical significance in human osteosarcoma. The expression of HSPgp96 was studied in 44 osteosarcoma tissues including 24 osteoblastic sarcoma and 20 chondroblastic sarcoma, normal tissues adjacent to the sarcomas were evaluated simultaneously. The immunoreactivity was found positive in all osteosarcoma tissues (44/44), but 21.5% (9/44) in normal tissues. HSPgp96 was mainly expressed in cytoplasm of osteoblastic sarcoma, while in nucleus of chondroblastic sarcoma. HSPgp96 immunolabelling had ...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Guo WC, Zhao SH, Yu L, Tang J, Wu GX, Chen JL Tags: Neoplasma Source Type: journals

The utility of 99mTc-EDDA/HYNIC-TOC scintigraphy for assessment of lung lesions in patients with neuroendocrine tumors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Our aim was to assess clinical utility of 99mTc-EDDA/HYNIC-TOC scintigraphy for evaluation of lung lesions in patients with neuroendocrine tumors (NETs). Single photon emission computed tomography (SPECT) of the thorax and whole body scintigraphy were performed in 34 patients using 99mTc-EDDA/HYNIC-TOC. Visual assessment was complemented by semiquantitative evaluation based on tumor to non-tumor (T/NT) ratio. Clinical, laboratory, and histological findings served as the standard for comparison. Enhanced tracer uptake was observed on both SPECT and whole body scintigraphy in 29 of 34 patients (88% sensitivity). T/NT rat...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Pavlovic S, Artiko V, Sobic-Saranovic D, Damjanovic S, Popovic B, Jakovic R, Petrasinovic Z, Jaksic E, Todorovic-Tirnanic M, Saranovic D, Micev M, Novosel S, Nikolic N, Obradovic V Tags: Neoplasma Source Type: journals

Relationship between plasma progesterone, estradiol and prolactin concentrations and breast cancer in pre and postmenopausal women.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hormones such as estrogen, progesterone and prolactin are implicated in anumber of ways as possible causes of breast cancer. Throughout women life cycle, breast development and function depend on complex critical interplay of these hormones. The acknowledged gaps in our understanding concerning progesterone, estrogen and prolactin hormones involvement in human breast cancer has exposed the need to conduct this study for better understanding of the role played by these hormones in breast cancer during pre and post menopause status in order to influence prevention and treatment of breast cancer. Ninety women were enrolle...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Raheem SN, Atoum M, Al-Hourani H, Rasheed M, Nimer N, Almuhrib T Tags: Neoplasma Source Type: journals

Therapeutic effect of photodynamic therapy using hematoporphyrin monomethyl ether (HMME) on human cholangiocarcinoma cell line QBC939.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the effect of PDT using HMME as the photosensitizer, and the 630nm diode laser on human cholangiocarcinoma cell line QBC939. Cell viability was determined by MTT assay. The percentage of apoptotic cell was determined by flow cytometry following annexin V/PI staining. Two methods were used for the determination of apoptosis: terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling assay and laser scanning confocal microscope detection. The procaspase-3 and cytochrome cwere measured by western blot. In vitro PDT showed excellent cytotoxicity that was afunction of laser energy and drug concentra...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Wang JB, Liu LX, Pan SH, Wang CY, Fu QF Tags: Neoplasma Source Type: journals

Polymorphism in heme oxygenase-1 (HO-1) promoter and alcohol are related to the risk of esophageal squamous cell carcinoma on Chinese males.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the correlation between the HO-1 gene promoter polymorphism and alcohol, along with the risk of ESCC on Chinese males, was analyzed.The case-control study was performed in 143 ESCC patients and 264 cancer-free controls. All subjects were males. Allelotypic frequencies of (GT)n repeat were examed by PCR-based genotyping and DNA sequencing. The frequencies of L-allele and L-allele carriers (S/L and L/L genotypes) was significantly higher in ESCC patients than in controls (p =0.001 and 0.004), The adjusted ORs for ESCC with S/L and L/L genotypes vs S/S genotype was 2.212 (95% CI 1.297-3.775, p= 0.004). The adju...
Source: Neoplasma - November 11, 2009 Category: Cancer & Oncology Authors: Hu JL, Li ZY, Liu W, Zhang RG, Li GL, Wang T, Ren JH, Wu G Tags: Neoplasma Source Type: journals

Depression symptoms and health-related quality of life among patients with metastatic breast cancer in programme of palliative cancer care.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was local prospective and cross-sectional. It was carried at Department of Clinical Oncology and Radiation Therapy of Charles University Hospital in Hrader Králové, Czech Republic. Dates were obtained during year 2008 among 41 patients with metastatic breast cancer in programme of palliative cancer care. The mean age for all 41 subjects was 58 years old (aged 41 - 80 years old). The Czech version of Zung self-rating depression scale was performed for evaluation of depression symptoms. The Czech version of genetic EuroQol questionnaire EQ-5D was performed for evaluation of level of HRQoL. The statistica...
Source: Neoplasma - November 2, 2009 Category: Cancer & Oncology Authors: Slovacek L, Slovackova B, Slanska I, Petera J, Priester P, Filip S, Kopecky J Tags: Neoplasma Source Type: journals

Damage of hormonal function and bone metabolism in long-term survivors of testicular cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Improved survival of testicular cancer (TC) patients leads to rising of interest on the disease consequences for the whole organism (impact on hormonal status, bone metabolism). The aim of the study was to present three years experience with hormonal and osteologic examination in long-term survivors of TC. During the period of 11/2005-1/2009 879 patients who were previously treated for TC (823 with unilateral, 56 with bilateral disease) were examined. Each patient underwent hormonal and osteologic examination, results of which were associated with therapy following orchiectomy and with the time interval since the prima...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Ondrusova M, Ondrus D, Dusek L, Spanikova B Tags: Neoplasma Source Type: journals

Long-term survival of patients treated for Hodgkin's disease in 1971-1996 depending on the clinical stage of the disease, patient's fertility after therapy and cause of death analysis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The long-term survival in a group of 370 patients treated for Hodgkin's disease in the years 1971-1996 was retrospectively analyzed. Up to now 191 patients live. 179 patients have died. Since the year 1978 the uniform diagnostic and therapy protocol has been used. The therapy consisted in the combination of a radiation therapy (usual dose 40 Gy) with the COPP chemotherapy (6 cycles). Since the year 1988 the alternation of the ABVD and COPP chemotherapy has been used. According to the stage of the Hodgkin's disease and the patient's age the modification of the therapy was introduced. One chemotherapy cycle wa...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Fridrichova M, Dienstbier Z, Loucka M, Skala E, Blomannova E Tags: Neoplasma Source Type: journals

Different phenotype manifestation of familial adenomatous polyposis in families with APC mutation at codon 1309.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Germline mutation in APC gene induced development of familial adenomatous polyposis (FAP). The risk of developing specific manifestation of FAP is often correlated with the position of the inherited APC mutation. Patients with mutations localized in the largest exon 15 between codons 1286 and 1513 (mutation cluster region, MCR) have generally a worse prognosis with early onset of the disease. We found 6 FAP families with mutation at codon 1309 (3927_3931delAAAGA) in the cohort of 39 FAP Slovak families with rapid cancer progress. In addition, mutation in codon 1309 was detected in three family members, one of them with...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Wachsmannova-Matelova L, Stevurkova V, Adamcikova Z, Holec V, Zajac V Tags: Neoplasma Source Type: journals

Cell cycle analysis and cytotoxic potential of Ruta graveolens against human tumor cell lines.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
There are reports on the presence of various compounds exerting different biological activities in Ruta graveolens, a plant of Rutaceae family. The aim of the present study was to evaluate in vitro cytotoxicity of the total extract of R. graveolens against tumor cell lines of different origin. Aerial parts of the plant was extracted with 70% ethanol by sonication method and cytotoxic activity was examined on RAJI, RAMOS, RPMI8866, U937, Jurkat, MDA-MB-453, MCF-7, LNCap-FGC-10, 5637, HeLa, SK-OV-3, A549, Mehr-80 and also peripheral blood mononuclear cells (PBMC) by the use of WST-1 assay. Results were expressed as IC50 ...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Varamini P, Soltani M, Ghaderi A Tags: Neoplasma Source Type: journals

3-(5-Nitro-2-thienyl)-9-chloro-5-morpholin-4-yl[1,2,4]triazolo[4,3-c]quinazoline induces ROS-mitochondrial mediated death signaling and activation of p38 MAPK in murine L1210 leukemia cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Quinazoline derivatives are multitarget agents with a broad spectrum of biological activity. 3-(5-nitro-2-thienyl)-9-chloro-5-morpholin-4-yl[1,2,4]triazolo[4,3-c]quinazoline (NTCHMTQ) is a new synthetically prepared derivative, which in our previous studies showed antiproliferative and apoptosis inducing activities towards murine L1210 leukemia cells. The aim of this study was to provide the insight into the molecular mechanism regulating NTCHMTQ-induced apoptosis in L1210 cells. The activity of caspases 3, 8 and 9, generation of reactive oxygen species (ROS), mitochondrial membrane potential changes, release of cytoch...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Jantova S, Repicky A, Cipak L Tags: Neoplasma Source Type: journals

Germline variants of the promyelocytic leukemia tumor suppressor gene in patients with familial cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, germline disruption of the PML gene is probably not associated with a highly penetrant susceptibility to adult-onset breast and colon cancer. Pathogenicity of c.83C>T and c.1558C>T variants in the PML gene is uncertain. Carriers of the c.2260 G>C variant in PMLIV isoform may be at an increased risk of colon polyposis and cancer. Keywords: breast cancer; cancer risk; colon cancer; colon polyposis; germline mutation; PML gene. PMID: 19728758 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Plevova P, Walczyskova S, Jeziskova I, Jurckova N, Krepelova A, Puchmajerova A, Pavlikova K, Foretova L, Zapletalova J, Silhanova E Tags: Neoplasma Source Type: journals

Immunophenotyping parameters as prognostic factors in T-acute leukemia patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The main aim of this study represents the extension of our studies using multiparametric flow cytometry analysis for exact definition of membrane and intracellular (cytoplasmic and nuclear) markers of acute leukemia cells of T-phenotype. The study of blasts of each patient with all available monoclonal antibodies targeted to T-cell differential antigens and against possible marker coexistence from different lineages has been performed. The main aim was concerned to more proper T-ALL diagnosis and stage definition and identification of the prognostic factors and the useful markers for the follow-up of T-ALL in remission...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Babusikova O, Stevulova L, Fajtova M Tags: Neoplasma Source Type: journals

Activation of HLA-G expression by 5-aza-2 - deoxycytidine in malignant hematopoetic cells isolated from leukemia patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human leukocyte antigen - G (HLA-G) is a non-classical HLA class I antigen with restricted distribution in normal tissues. Ectopic HLA-G expression observed at some pathological circumstances as malignant transformation might be triggered by epigenetic modifications such as DNA demethylation. Recently it was demonstrated that DNA methyltransferase inhibitor 5-aza-2 - deoxycytidine (AdC) induces/enhances HLA-G transcription in many leukemia cell lines of different origin. Here we investigated the effect of AdC on HLA-G expression in malignant hematopoetic cells isolated from patients with acute myeloid leukemia (AML) an...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Polakova K, Bandzuchova E, Sabty FA, Mistrik M, Demitrovicova L, Russ G Tags: Neoplasma Source Type: journals

Hormonal and metabolic evaluation of adrenal incidentalomas.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we confirmed a higher prevalence of symptoms characteristic for different metabolic syndromes in these patients with adrenal incidentalomas, which indicate systematic screening for the metabolic syndrome including evaluation of the insuline resistance in this patients. Keywords: adrenal incidentaloma, cholesterol, triacyglyceroles, obesity, hypertension, steroid enzymopathy. PMID: 19728761 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Wagnerova H, Dudasova D, Lazurova I Tags: Neoplasma Source Type: journals

Phenotype of plasma cells in multiple myeloma and monoclonal gammopathy of undetermined significance.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Flow cytometry is a useful tool for the analysis of plasma cells in monoclonal gammopathies. The aim of this study was to find possibilities and limits of multicolour flow cytometry in diagnostics of monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM) and to identify parameters that could be used to differentiate between these two disorders. Surface markers CD38 and CD138 were used for identification of plasma cells, CD19 and CD56 further distinguished normal and abnormal plasma cells, respectively. The percentage of circulating plasma cells in peripheral blood was lower in MGUS patients...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Kovarova L, Buresova I, Buchler T, Suska R, Pour L, Zahradova L, Penka M, Hajek R Tags: Neoplasma Source Type: journals

Expression of Notch1, Jagged1 and beta-catenin and their clinicopathological significance in hepatocellular carcinoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, Notch1/Jagged1 were frequently low expressed in hepatocellular carcinoma and correlated with the high expression of beta-catenin suggesting that downregulation of Notch1/Jagged1 signaling may sustain tumor progression. Keywords: Notch1, Jagged, beta-catenin; hepatocellular carcinoma, tissue microarrays, immunohistochemistry. PMID: 19728763 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Wang M, Xue L, Cao Q, Lin Y, Ding Y, Yang P, Che L Tags: Neoplasma Source Type: journals

Genotoxic damage of human adipose-tissue derived mesenchymal stem cells triggers their terminal differentiation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human adipose tissue-derived mesenchymal (stromal) stem cells (AT-MSCs) and genetically modified to express cytosine deaminase:uracil phosphoribosyltransferase (CDy-AT-MSCs) were treated with hydrogen peroxide in order to induce DNA damage and subsequently evaluate their genetic stability by single cell gel electrophoresis. Both cells types (parental and transgene modified) did not differ in the sensitivity to DNA breaks induction. Potential tumorigenicity of AT-MSCs and CDy-AT-MSCs was tested by subcutaneous inoculation of cell suspension into flank of immunocompromised mice. Dose of 15x106 cells was not found to be t...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Altanerova V, Horvathova E, Matuskova M, Kucerova L, Altaner C Tags: Neoplasma Source Type: journals

Modulation of markers associated with aggressive phenotype in MDA-MB-231 breast carcinoma cells by sulforaphane.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we describe effect of SFN on various aspects determining invasive behavior of MDA-MB-231 human breast carcinoma cells. We studied modulation of molecules associated with epithelial to mesenchymal transition (EMT), hypoxic marker CA IX and mitochondrially located peripheral benzodiazepine receptor (PBR) using flow cytometry, gene expression of matrix metalloproteinases MMP1, 3, 7, 9, 14, transcription factors POU5F1 and Twist1 mRNA by RT PCR, and cytokine production by multiplex bead assay. SFN downregulated PBR and vimentin expression in a dose dependent manner, but significantly affec...
Source: Neoplasma - September 5, 2009 Category: Cancer & Oncology Authors: Hunakova L, Sedlakova O, Cholujova D, Gronesova P, Duraj J, Sedlak J Tags: Neoplasma Source Type: journals

A side population of cells from a human pancreatic carcinoma cell line harbors cancer stem cell characteristics.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We used flow cytometry and a DNA-binding dye efflux assay to isolate a side population (SP) of cells with stem cell characteristics from the human pancreatic carcinoma cell line, PANC-1. Non-obese diabetic/severe combined immunodeficiency mouse xenograft experiments showed that SP cells were enriched in tumor initiating capability compared with non-SP cells. Cultured SP cells were able to differentiate into daughter cells and non-SP cells, through asymmetric division. Our study demonstrated that SP cells had high drug-resistance, both in vivo and in vitro. SP cells also showed significantly higher levels of mRNA expres...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Wang YH, Li F, Luo B, Wang XH, Sun HC, Liu S, Cui YQ, Xu XX Tags: Neoplasma Source Type: journals

Reduced inducibility of SOCS3 by interferon gamma associates with higher resistance of human breast cancer lines as compared to normal mammary epithelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The resistance to interferons (IFNs) limits their anticancer therapeutic efficacy. Here we studied the antiproliferative effect of interferon gamma in relation to SOCS3 expression in a panel of breast cancer cell lines and normal mammary epithelial cells. Compared to normal cells most breast cancer lines (7/8) were highly resistant to IFN-gamma. Using Northern blot and real time RT-PCR we investigated transcription of SOCS3 genes. All normal epithelial cells (4/4) showed SOCS3 induction (2-14 fold) while most breast cancer lines did not or weakly activated SOCS3 after the interferon gamma treatment. Among the cancer li...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Souckova K, Kovarik A, Dusek L, Humpolikova-Adamkova L, Lauerova L, Krejci E, Matouskova E, Bursikova E, Fojtova M, Kovarik J Tags: Neoplasma Source Type: journals

Brazilein inhibits survivin protein and mRNA expression and induces apoptosis in hepatocellular carcinoma HepG2 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hepatocellular carcinomas represent the third leading cause of cancer-related deaths worldwide. Survivin, a structurally unique member of the inhibitor of apoptosis protein (IAP) family, is overexpressed in a wide range of malignancies, including hepatocellular carcinoma. Due to its involvement in cancer progression and treatment resistance, survivin is currently undergoing extensive investigation as a novel intervention target to induce apoptosis in cancer cells by phytochemicals or synthetic agents. Brazilein, a compound obtained in a large amount from the dried heartwood of Caesalpinia sappan Linn., which has long b...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Zhong X, Wu B, Pan YJ, Zheng S Tags: Neoplasma Source Type: journals

WT1 expression in peripheral leukocytes of patients with chronic myeloid leukemia serves for the prediction of Imatinib resistance.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The determination of patient's resistance to a particular drug contributes to more efficient therapeutical approach. The aim of this study was to evaluate if the responsiveness of Chronic Myeloid Leukemia (CML) patients to Imatinib therapy was predictable from WT1 gene expression in peripheral blood leukocytes. To examine the resistance we implemented an in vitro cultivation of the primary cells of 48 CML patients with Imatinib. The effect of Imatinib was characterized not only by the expression of WT1 but also by BCR-ABL, and proliferative factor Ki-67. Our results showed that leukocytes of CML patients, c...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Otahalova E, Ullmannova-Benson V, Klamova H, Haskovec C Tags: Neoplasma Source Type: journals

Kinetics of tumor marker decline as an independent prognostic factor in patients with relapsed metastatic germ-cell tumors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Early serum tumor marker decline (STMD) during chemotherapy was shown to predict survival in patients with poor prognosis non-seminomatous germ cell tumors (GCT) in the first line. The aim of the study was to assess the prognostic value of STMD in relapsed GCT;s patients. From January 1995 to December 2007, all patients treated for GCT s with salvage therapy at the National Cancer Institute of Slovakia were identified from the tumor registry database and screened retrospectively for serum AFP and betaHCG level at the time of relapse. STMD rate was calculated for each patient and each tumor marker with an abnormal marke...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Mego M, Rejlekova K, Reckova M, Sycova-Mila Z, Obertova J, Rajec J, Mardiak J Tags: Neoplasma Source Type: journals

Nuclear topography of the 1q21 genomic region and Mcl-1 protein levels associated with pathophysiology of multiple myeloma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the lymphoblastoid cell line CD138- ARH-77, multiple myeloma CD138+ MOLP-8 cells, and the CD138+ bone marrow fraction of patients diagnosed with MM. In this experimental system, we observed that gamma-radiation and selected cytostatic drugs such as melphalan and dexamethasone did not significantly alter the nuclear radial arrangement of the 1q21 region and other relevant regions of chromosome 1. Similarly, conserved nuclear radial positioning after cytostatic treatment was observed for the c-myc, TP53, CCND1, and IgH loci. When analyzed Mcl-1, a protein encoded by a gene mapped to the 1q21 region, we found that...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Legartova S, Krejci J, Harnicarova A, Hajek R, Kozubek S, Bartova E Tags: Neoplasma Source Type: journals

Immunohistochemical markers of proliferation and vascularisation in preneoplastic bronchial lesions and invasive non-small cell lung cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Autofluorescence bronchoscopy (AFB) has been shown to be sensitive to detect preneoplastic lesions in central lung airways system. In early stages of carcinogenesis, up-regulation of cyclooxygenase (COX)-2, Ki67 and/or increased angiogenesis may play a role by promoting the proliferation of tumoral cells and their resistance to apoptosis, as well as angiogenesis, tumor cell invasion and setting up of the metastatic process. The present study compared the expression of proliferative (COX-2, Ki67 and PCNA) and angiogenic markers (CD34 and NG2) between preneoplastic bronchial squamous dysplasia lesions and invasive squamo...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Cavarga I, Kocan P, Boor A, Belak J, Zak V, Kluchova Z, Slavik L, Tkacova R Tags: Neoplasma Source Type: journals

Polymorphisms of biotransforming enzymes (GSTs) and their association with colorectal cancer in the Slovak population.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of present study was to summarize the results of a case-control study focused on genetic polymorphisms of selected Phase II metabolizing enzymes (GSTM1, T1, P1) and to investigate the association of these polymorphisms with the colorectal cancer risk among the Slovak population. A case-control study with 183 colorectal cancer cases and 422 controls was conducted. DNA was extracted from peripheral blood leukocytes, and the polymorphisms of GSTM1, GSTT1 and GSTP1 enzymes were determined by PCR-based methods. Association between specific genotypes and the development of colorectal cancer were examined ...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Matakova T, Sivonova M, Halasova E, Mistuna D, Dzian A, Masar J, Dobrota D Tags: Neoplasma Source Type: journals

Acute lymphoblastic leukemia-derived dendritic cells express tumor associated antigens: PNPT1, PMPCB, RHAMM, BSG and ERCC1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In all types of leukemia both in children and adults there is a need for novel therapies that could reduce the risk of relapse after standard treatment. Acute lymphoblastic leukemia (ALL) cells are ineffective antigen presenting cells, but as shown by many authors including results from our laboratory, stimulation with CD40L restores their antigen expressing capacity. The development of T-cell therapies for leukemic patients can be based on discovery of leukemia-associated antigens (LAA) which could be recognized by the host immune system. The aim of our present study was to test the hypothesis that leukemia-derived de...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Luczynski W, Kowalczuk O, Stasiak-Barmuta A, Ilendo E, Krawczuk-Rybak M, Chyczewski L Tags: Neoplasma Source Type: journals

Variation of bone marrow cd34+ cell subsets in myelodysplastic syndromes according to who types.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the major cell types found in the blast gate by flow cytometry in newly diagnosed patients with MDS, compared them to normal BM and studied their variation according to WHO type. Two subsets defined by SSC were found both in normal BM and MDS, corresponding to myeloblasts and B-cell precursors. The number of B-cell precursors among all nucleated cells was equally low, independent of WHO type. However, the subset with an intermediate SSC, but CD117, CD13 and CD19 negative increased with the rise of myeloblasts. Concomitantly, the ratio between CD34+/CD117+/CD34-/CD117+ cells was increased. These two features are...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Reis SC, Traina F, Metze K, Saad ST, Lorand-Metze I Tags: Neoplasma Source Type: journals

Associations between hypoxia-inducible factor-1alpha (HIF-1alpha) gene polymorphisms and risk of developing breast cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The C1772T, G1790A and C111A polymorphisms of Hypoxia-inducible factor-1alpha (HIF-1alpha) gene were analyzed in a hospital-based Malaysian population using PCR-RFLP method. Genomic DNA was extracted from the blood samples collected from 410 breast cancer patients and 275 normal and healthy women. We investigated the association between HIF-1alpha polymorphisms and breast cancer risk, and clinico-pathological parameters in the population. The genotype and allele frequencies of C1772T (P=0.0093 vs P=0.0024) polymorphism were significantly different between the breast cancer cases and normal subjects but similar associat...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Naidu R, Har YC, Taib NA Tags: Neoplasma Source Type: journals

Estrogen-dependent Regulation of PPAR-gamma Signaling on Collagen Biosynthesis in Adenocarcinoma Endometrial Cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The link between estrogen and metabolic developmental factors of endometrial carcinoma is well established. PPAR- gamma, (an important modulator of metabolism) and estrogen receptor belong to a family of nuclear hormone receptors that were shown to interact with each other. The interaction may affect transcriptional activity of these transcription factors. The anti-diabetic troglitazone (TGZ) is well known PPAR- gamma ligand. The effect of troglitazone-induced PPAR- gamma activation on estrogen-dependent stimulation of collagen biosynthesis was studied in the Ishikawa endometrial adenocarcinoma cell line. We have found...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Surazynski A, Jarzabek K, Miltyk W, Wolczynski S, Palka J Tags: Neoplasma Source Type: journals

Clonal evolution in chronic lymphocytic leukemia studied by interphase fluorescence in-situ hybridization.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The results of repeated interphase fluorescence in-situ hybridization (I-FISH, FISH) examination of 97 CLL patients and correlation of these findings with IgVH hypermutation status, ZAP-70 and CD38 expression are presented. The appearance of new, FISH-detectable, genomic aberrations during disease course, described as clonal evolution (CE), was observed in 26% of patients. The most frequent newly acquired cytogenetic abnormality was 13q deletion in 64% (16/25). In contrast to earlier studies, there was no correlation found between CE and either one of single negative prognostic factors (unmutated IgVH; CD38 positivity;...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Berkova A, Zemanova Z, Trneny M, Schwarz J, Karban J, Cmunt E, Pavlistova L, Brezinova J, Michalova K Tags: Neoplasma Source Type: journals

A whole population study of gastrointestinal stromal tumors in the Czech Republic and Slovakia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Due to problems with identification and an incomplete understanding on the gastrointestinal stromal tumors (GIST) before 2001, there has been a lack of comprehensive long-term population-based studies on GIST epidemiology at present date. We used data from the online registry of Czech and Slovak GIST patients (http://gist.registry.cz/), which has been compiled and maintained since 2006 and involves patients diagnosed from the year 2000. 278 patients were included in this study. Most of the tumors fell into the high-risk category (58.7%), followed by the intermediate (21.4%), low (16.6%) and very low (3.3%) categories. ...
Source: Neoplasma - July 8, 2009 Category: Cancer & Oncology Authors: Brabec P, Sufliarsky J, Linke Z, Plank L, Mrhalova M, Pavlik T, Klimes D, Gregor J Tags: Neoplasma Source Type: journals

The Role of PPARs in MDR - alesson from embryonic development.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
One of the most important features of embryonic cells is their resistence to xenobiotics, which provides a natural protection for embryos against these potentially harmfull molecules. In this way, embryo cells resemble cancer cells and thus understanding the basis of this phenomenon may contribute to overcoming the multi-drug-resistance (MDR) of some tumours. Peroxisome proliferator-activated receptors (PPARs) are steroid nuclear receptors that regulate diverse biological processes such as lipid and carbohydrate metabolism, development, differentiation, apoptosis, neoplastic transformation, inflammation and regeneratio...
Source: Neoplasma - June 27, 2009 Category: Cancer & Oncology Authors: Konieczna A, Lichnovka R, Erdosova B, Ehrmann J Tags: Neoplasma Source Type: journals

17beta-hydroxysteroid dehydrogenase type 11 (Pan1b) expression in human prostate cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we evaluated immunolocalization of Pan1b in human prostate cancer specimens obtained from surgery (n=40), and correlated the findings with clinicopathological features of the patients in order to study its clinical significance. Pan1b immunoreactivity was detected in 19 cases (48%) and was significantly associated with cancer of seminal vesicle invasion (P Keywords: 17ss-hydroxysteroid dehydrogenase type XI, prostate cancer, immunochemistry. PMID: 19469652 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - June 27, 2009 Category: Cancer & Oncology Authors: Nakamura Y, Suzuki T, Arai Y, Sasano H Tags: Neoplasma Source Type: journals

Low frequency mutation of the Ephrin receptor A3 gene in hepatocellular carcinoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
EphA3 is acomponent of the Eph/ephrin tyrosine kinase system, which participates in vasculature development. This receptor/ligand system is associated with various signaling pathways related to cell growth and viability, cytoskeletal organization, cell migration, and anti-apoptosis. Accumulated evidence suggests that aberrant regulation of EphA3 and its genetic alterations are implicated in the development and progression of various cancers. However, despite ahigh incidence of EphA3 over-expression, no such investigation has been performed in hepatocellular carcinoma. Thus, we investigated genetic alterations of the Ep...
Source: Neoplasma - June 27, 2009 Category: Cancer & Oncology Authors: Bae HJ, Song JH, Noh JH, Kim JK, Jung KH, Eun JW, Xie HJ, Ryu JC, Ahn YM, Kim SY, Lee SH, Yoo NJ, Lee JY, Park WS, Nam SW Tags: Neoplasma Source Type: journals

Differential gene expression of bone marrow CD34+ cells in early and advanced myelodysplastic syndrome.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study demonstrates stage-specific expression of some genes that may have potential prognostic significance. Keywords: gene expression profiling, myelodysplastic syndrome, CD34+ cells. PMID: 19469654 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - June 27, 2009 Category: Cancer & Oncology Authors: Vasikova A, Budinska E, Belickova M, Cermak J, Bruchova H Tags: Neoplasma Source Type: journals

The Glu346Lys polymorphism and frameshift mutations of the Methyl-CpG Binding Domain 4gene in gastrointestinal cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the MSI, frameshift mutations and polymorphisms of the MBD4 gene in 84 patients with gastric cancers, 82 colorectal cancers and 299 healthy controls. MSI was found in 19 (22.6%) and 26 (31.7%) of the gastric and colorectal cancer samples, respectively. The mutation analysis revealed no frameshift mutations in the MBD4 gene among the gastrointestinal cancers. The frequencies of genotypes: Glu/Glu, Glu/Lys and Lys/Lys were 41.7% (35/84), 41.7% (35/84) and 16.6% (14/84), respectively, in the gastric cancer cases, and 42.7% (35/82), 36.6% (30/82) and 20.7% (17/82), respectively, in the colorectal cancers. MSI was n...
Source: Neoplasma - June 27, 2009 Category: Cancer & Oncology Authors: Song JH, Maeng EJ, Cao Z, Kim SY, Nam SW, Lee JY, Park WS Tags: Neoplasma Source Type: journals

TNF-alpha-308 promotor polymorphism in patients with chronic obstructive pulmonary disease and lung cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chronic obstructive pulmonary disease (COPD) and lung cancer (LC) are amajor cause of morbidity and mortality worldwide. In both diseases airways inflammation plays an important role. Functional promoter polymorphism, at the position -308, of tumor necrosis factor (TNF)-alpha represents attractive potential susceptibilty marker for both diseases. In order to investigate the role of this polymorphism in COPD and LC, acase-control study was performed. The patient groups consisted of 97 subjects with COPD and 70 subjects with LC, while the control group encompassed 102 subjects. Results of our study showed significant dec...
Source: Neoplasma - June 27, 2009 Category: Cancer & Oncology Authors: Stankovic MM, Nestorovic AR, Tomovic AM, Petrovic-Stanojevic ND, Andjelic-Jelic MS, Dopudja-Pantic VB, Nagorni-Obradovic LjM, Mitic-Milikic MM, P Radojkovic D Tags: Neoplasma Source Type: journals

Human exposure to polyhalogenated hydrocarbons and incidence of selected malignancies -central European experience.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This paper describes results of two ecological studies design to analyze the incidence of selected malignancies in two populations exposed to polychlorinated hydrocarbons, mostly PCBs and TCDDs/Fs by comparing data available in the National Cancer Registry of the Slovak Republic and National Oncological Registry of the Czech Republic databases for the Slovak Republic (~ 5M inhabitants) and the Czech Republic (10,3 Minhabitants) to the data relevant for the population of Michalovce District, the Slovak Republic (~ 112,000 inhabitants) and Uherske Hradiste, the Czech Republic (146,000 inhabitants). Those districts are re...
Source: Neoplasma - June 27, 2009 Category: Cancer & Oncology Authors: Bencko V, Rames J, Ondrusova M, Plesko I, Jurickova L, Trnovec T Tags: Neoplasma Source Type: journals

HSP27 diagnostic utility in the fine needle aspirate of breast. Correlation with progesterone and estrogen receptors.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Fine needle aspiration (FNA) is routine diagnostic tool in breast tumors assessment. In some cases, however, limitations of this method do not permit an unequivocal diagnosis. In these, suspected, cases immunocytochemical evaluation of selected biological markers may be of help. The aim of the study was assessment of HSP27 value in diagnosis and discrimination of benign and malignant breast lesions. HSP27 expression was examined by immunocytochemistry in fine needle aspiration smears assessed to C2-C5 categories. In C5 subgroup HSP27 expression was correlated with ER, PR content. Statistically significant differences i...
Source: Neoplasma - June 27, 2009 Category: Cancer & Oncology Authors: Mlynarczyk-Liszka J, Maksymiuk B, Ponikiewska D, Krzyzowska-Gruca S, Lange D, Krawczyk Z, Malusecka E Tags: Neoplasma Source Type: journals