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129 records returned

Exploring epitopes of antibodies towards the human tryptophanyl-tRNA synthetase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
There is a need to characterise the epitopes of affinity reagents in order to develop high quality affinity reagents for research, diagnostics and therapy. Here, we describe the analysis of epitopes of antibodies generated towards human tryptophanyl-tRNA synthetase (WARS) using both combinatorial bacterial display and suspension bead array. The bacterial display revealed that the polyclonal antibody binds to three separate epitopes and peptide scanning using 15-mers revealed binding to a 13 amino acid consensus sequence (ELINRIERATGQR). A mouse monoclonal antibody was generated and the mapping approach revealed binding...
Source: New Biotechnology - November 10, 2009 Category: Biotechnology Authors: Hjelm B, Fernández CD, Löfblom J, Ståhl S, Johannesson H, Rockberg J, Uhlén M Tags: N Biotechnol Source Type: journals

A single Phe54Tyr substitution improves the catalytic activity and thermostability of Trigonopsis variabilis D-amino acid oxidase.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The industrial importance of Trigonopsis variabilis D-amino acid oxidase (TvDAAO) is represented by its biocatalytic oxidative deamination of cephalosporin C (CPC) to yield glutaryl-7-aminocephalosporanic acid (GL-7-ACA). The process has been incorporated into a two-step bioconversion to produce 7-aminocephalosporanic acid, the crucial synthetic nucleus for a number of semi-synthetic cephalosporin antibiotics. A homology model of TvDAAO indicated that residue F54 is in a close proximity to the insilico docked CPC. Substitution of this F54 to Tyr (F54Y) resulted in 6-fold improvement in k(cat,app) and approximately 2.5-...
Source: New Biotechnology - November 9, 2009 Category: Biotechnology Authors: Wong KS, Fong WP, Tsang PW Tags: N Biotechnol Source Type: journals

"Why the growing stakeholder consensus on technology transfer?" Commentary and Meeting Report on The European Tech Transfer Summit.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19895909 [PubMed - as supplied by publisher] (Source: New Biotechnology)
Source: New Biotechnology - November 3, 2009 Category: Biotechnology Authors: Suojanen C, Berrie M Tags: N Biotechnol Source Type: journals

Towards Proteome Scale Antibody Selections using Phage Display.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In vitro antibody generation by panning a large universal gene library with phage display was employed to generate antibodies to over 60 different antigens. Of particular interest was a comparison of pannings on 20 different SH2 domains provided by the Structural Genomics Consortium (SGC). Streamlined methods for high throughput antibody generation developed within the 'Antibody Factory' of the German National Genome Research Network (NGFN) were demonstrated to minimise effort and provide a reliable and robust source for antibodies. For the SH2 domains, in two successive series of selections, 2668 clones were analysed,...
Source: New Biotechnology - October 30, 2009 Category: Biotechnology Authors: Mersmann M, Meier D, Mersmann J, Gräslund S, Helmsing S, Nilsson P, Hust M, Dübel S Tags: N Biotechnol Source Type: journals

Optimized synthesis of 1,3-dioleoyl-2-palmitoylglycerol rich triacylglycerol via interesterfication catalyzed by a lipase from Thermomyces lanuginosus.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
1,3-Dioleoyl-2-palmitoylglycerol (OPO) rich human milk fat substitute (HMFS) was synthesized from tripalmitin rich fraction and ethyl oleate by a lipase-catalyzed interesterification. Response surface methodology was employed to optimize its OPO content and acyl migration with reaction factors - substrate mole ratio of PPP-rich fraction to ethyl oleate (1:4, 1:5 and 1:6), reaction temperature (50, 55 and 60 degrees C) and time (3, 7.5 and 12h). The predictive models for OPO content and acyl migration were adequate and reproducible. The OPO content increased with substrate ratio, and decreased with reaction time and tem...
Source: New Biotechnology - October 29, 2009 Category: Biotechnology Authors: Lee JH, Son JM, Akoh CC, Kim MR, Lee KT Tags: N Biotechnol Source Type: journals

Comparison of pretreatment strategies for enzymatic saccharification and fermentation of barley straw to ethanol.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Barley straw used in this study contained 34.3% cellulose, 23.0% hemicellulose, and 13.3% lignin (moisture, 6.5%). Several pretreatments (dilute acid, lime, and alkaline peroxide) and enzymatic saccharification procedures were evaluated for the conversion of barley straw to monomeric sugars. The maximum release of sugars (glucose, 384mg; xylose, 187mg; arabinose, 32mg; total sugars, 604mg per g; 94% of maximum theoretical sugar yield) from barley straw (10%, w/v) was obtained by alkaline peroxide (2.5% H(2)O(2), pH 11.5) pretreatment (35 (o)C, 24h) and enzymatic saccharification (45 (o)C, pH 5.0, 120h) after diluting 2...
Source: New Biotechnology - October 26, 2009 Category: Biotechnology Authors: Saha BC, Cotta MA Tags: N Biotechnol Source Type: journals

Specific detection of benzimidazole resistance in Colletotrichum gloeosporioides from fruit crops by PCR-RFLP.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Anthracnose diseases, caused by Colletotrichum gloeosporioides, are a worldwide problem and are especially important in Taiwan due to the severe economic damage they cause to tropical fruits that are grown for local consumption and export. Benzimidazoles are systemic fungicides widely used for controlling these diseases in Taiwan. Thirty-one isolates of C. gloeosporioides from mango and strawberry grown in Taiwan were examined for their sensitivity to benzimidazole fungicides. The responses of the isolates grown on benzimidazole-amended culture media were characterized as sensitive, moderately resistant, resistant or h...
Source: New Biotechnology - October 22, 2009 Category: Biotechnology Authors: Chung WH, Chung WC, Peng MT, Yang HR, Huang JW Tags: N Biotechnol Source Type: journals

AMP-activated protein kinase: a potential target for the diseases prevention by natural occurring polyphenols.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A reduced life span is an outcome associated with many prevalent diseases, including diabetes, obesity, and high blood pressure. In seeking to prevent these diseases, many researchers have looked into potential therapeutic benefits of naturally occurring compounds. AMP-activated protein kinase (AMPK) is a major metabolic-sensing protein implicated in the prevention of metabolic disorders, or in minimizing the effects thereof, via the regulation of both upstream and downstream target molecules. In the field of food and nutrition, the current focus lies in the finding of components that activate AMPK. AMPK is a serine/th...
Source: New Biotechnology - October 14, 2009 Category: Biotechnology Authors: Hwang JT, Kwon DY, Yoon SH Tags: N Biotechnol Source Type: journals

Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestivum.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Triticum aestivum is of major importance both nutritionally and economically. Introduction of new genes has been difficult to apply to elite wheat varieties mainly as a result of their recalcitrance to prerequisite tissue culture. We attempted to improve the frequency of wheat transformation by exposing plants to high level of ammonium nitrate. Our experiments showed that modification of the ammonium nitrate content in the direct somatic embryogenesis induction medium can increase the number of primary embryos produced over twofold in the elite hard red wheat cultivar Superb. The number of primary embryos that were cap...
Source: New Biotechnology - October 14, 2009 Category: Biotechnology Authors: Greer MS, Kovalchuk I, Eudes F Tags: N Biotechnol Source Type: journals

Cell-associated acid beta-xylosidase production by Penicillium sclerotiorum.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In recent decades, beta-xylosidases have been used in many processing industries. In this work, the study of xylosidase production by Penicillium sclerotiorum and its characterization are reported. Optimal production was obtained in medium supplemented with oat spelts xylan, pH 5.0, at 30 degrees C, under stationary condition for six days. The optimum activity temperature was 60 degrees C and unusual optimum pH 2.5. The enzyme was stable at 50 and 55 degrees C, with half-life of 240 and 232min, respectively. High pH stability was verified from pH 2.0 to 4.0 and 7.5. The beta-xylosidase was strongly inhibited by divalen...
Source: New Biotechnology - October 14, 2009 Category: Biotechnology Authors: Knob A, Carmona EC Tags: N Biotechnol Source Type: journals

Screening and characterization of a phosphopentomutase useful for enzymatic production of 2'-deoxyribonucleoside.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bacillus sphaericus AKU 229 was found to produce an acetaldehyde-tolerant and phosphorylated compound-tolerant phosphopentomutase useful for enzymatic 2'-deoxyribonucleoside production. The gene encoding the phosphopentomutase was cloned and expressed in Escherichia coli. The E. coli expressing B. sphaericus phosphopentomutase was an excellent catalyst as to production of 2'-deoxyribonucleoside in the presence of acetaldehyde and phosphorylated compounds such as fructose 1,6-diphosphate, and d-glyceraldehyde 3-phosphate, which are derived from glucose through glycolysis with yeast cells, and exist abundantly in the pra...
Source: New Biotechnology - October 14, 2009 Category: Biotechnology Authors: Horinouchi N, Kawano T, Sakai T, Matsumoto S, Sasaki M, Mikami Y, Ogawa J, Shimizu S Tags: N Biotechnol Source Type: journals

Efficient enzymatic preparation of hydroxycinnamates in ionic liquids enhances their antioxidant effect on lipoproteins oxidative modification.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Biocatalytic lipophilization of hydroxycinnamic acids was performed in several BF(4)(-) and PF(6)(-) imidazolium ionic liquids using immobilized lipases. The influence of various reaction parameters on the performance of the biocatalytic process was pointed out, using as model reaction the esterification of ferulic acid. The biocatalytic lipophilization strongly depended on the ion composition of ionic liquids used. Conversions and initial reaction rates were significantly higher in PF(6)(-) as compared with BF(4)(-) ionic liquids and commonly used organic solvents. The high enzyme stability and the relative solubility...
Source: New Biotechnology - October 14, 2009 Category: Biotechnology Authors: Katsoura MH, Polydera AC, Tsironis LD, Petraki MP, Rajacić SK, Tselepis AD, Stamatis H Tags: N Biotechnol Source Type: journals

Production of oxygenated fatty acids from vegetable oils by Flavobacterium sp. strain DS5.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Flavobacterium sp. strain DS5 (NRRL B-14859) was used to convert two vegetable oils, olive oil and soybean oil, directly to oxygenated fatty acids such as 10-ketostearic acid (10-KSA) and 10-hydroxystearic acid (10-HSA). Lipase addition to the culture was required because strain DS5 did not induce lipase activity to release free fatty acids from vegetable oils. 10-KSA production was higher from olive oil than from soybean oil because olive oil contains more oleic acid, the precursor of 10-KSA. The optimum amounts of olive oil and lipase addition for 10-KSA production were determined as 0.3ml and 1mg (specific activity=...
Source: New Biotechnology - October 14, 2009 Category: Biotechnology Authors: Heo SH, Hou CT, Kim BS Tags: N Biotechnol Source Type: journals

Acute but not chronic exposure to abiotic stress results in transient reduction of expression levels of the transgene driven by the 35S promoter.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The transgenic plant performance depends on the stable expression of the integrated transgene. In this paper we have analyzed the stability of the most frequently used constitutive promoter, the cauliflower mosaic virus (CaMV) 35S promoter. We used several independent Nicotiana tabacum lines transgenic for the luciferase (LUC) or green fluorescence protein (GFP) coding genes driven by the same 35S promoter. As an indication of the expression level, we measured the steady state RNA level, protein level and protein activity. Exposure of plants to the acute single dose of UVC, UVB or X-ray radiation resulted in the decrea...
Source: New Biotechnology - September 28, 2009 Category: Biotechnology Authors: Boyko A, Molinier J, Chatter W, Laroche A, Kovalchuk I Tags: N Biotechnol Source Type: journals

Enhanced production of xylanase by Aspergillus carneus M34 in solid-state fermentation with agricultural waste using statistical approach.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The production of a low molecular weight xylanase by Aspergillus carneus M34 was investigated in solid-state fermentation using agricultural waste as the substrate. Experimental designs were practiced for optimization of the medium composition. When agricultural wastes of coba husk and corn steep liquor were used in the ratio of 4.5:0.5, a 22.5% increase of xylanase activity was observed compared to the medium containing only coba husk. The incubation time for xylanase production can be reduced from 12 days to 6 days by increasing the inoculum size to 2x10(7) spores mL(-1). The optimal media compositions were as follow...
Source: New Biotechnology - September 28, 2009 Category: Biotechnology Authors: Fang TJ, Liao BC, Lee SC Tags: N Biotechnol Source Type: journals

Isolation and Characterization of a Novel Thermostable alpha-Amylase from Korean Pine Seeds.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we firstly isolated and characterized a novel thermostable alpha-amylase from Korean pine seed. Enzyme was purified to homogeneity level with purification fold of 1286.1 using several techniques such as self-precipitation, (NH(4))(2)SO(4) fractionation, DEAE anion exchange and starch affinity chromatography. The purified alpha-amylase showed two bands in SDS-PAGE with molecular weight of 44kDa and 45kDa. The apparent molecular weight of native enzyme was calculated to be 46.7kDa. Internal peptide sequencing confirmed that the purified alpha-amylase was a novel enzyme. The optimum pH and temperature for enzyme...
Source: New Biotechnology - September 15, 2009 Category: Biotechnology Authors: Azad MA, Bae JH, Kim JS, Lim JK, Song KS, Shin BS, Kim HR Tags: N Biotechnol Source Type: journals

Yeast cultures with UCP1 uncoupling activity as a heating device.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Uncoupling proteins (UCPs) are mitochondrial transporters which facilitate controlled dissipation of the proton gradient and thus regulate energetic efficiency. The heat generating capacity of UCP from brown adipose tissue was investigated in yeasts expressing the protein recombinantly under conditions in which the temperature of the growth medium was measured directly. A Liquid Culture Calorimeter (LCC) was built consisting of a thermally isolated culture flask able to keep yeast cultures warm without resorting to additional heating. The exact internal temperature of the cultures was monitored for 24hours through a th...
Source: New Biotechnology - September 13, 2009 Category: Biotechnology Authors: Delás J, Notari M, Forés J, Pechuan J, Porcar M, Navarro E, Montagud A, Baguena M, Peretó J, Córdoba PF, González-Barroso MM, Rial E, Moya A, Urchueguía J Tags: N Biotechnol Source Type: journals

A Microsatellite (SSR) based linkage map of Brassica rapa.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study we describe the construction of a genetic linkage map for the Brassica rapa (AA) genome which will act as a key resource in undertaking future structural and functional genomic studies in B. rapa. A F(2) mapping population consisting of forty eight F(2) individual plants developed following hybridization of two inbred lines Bathari mandi and IC 331817 was used to construct the map. The map comprises a total of 53 SSR markers derived from three different public domain resources. Nine linkage groups along with a small subgroup were identified and designated as R1- R9 through alignment and orientation...
Source: New Biotechnology - September 13, 2009 Category: Biotechnology Authors: Kapoor R, Banga SS, Banga SK Tags: N Biotechnol Source Type: journals

Production of high level of cellulase-poor xylanases by wild strains of white-rot fungus Coprinellus disseminatus in solid-state fermentation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The production, optimisation and partial characterisation of xylanases from newly isolated wild strains of Coprinellus disseminatus was performed in solid-state fermentation. Strains SH-1 and SH-2 showed high xylanase (727.78 and 227.99 IU/mL) with very low CMCase (0.925 and 0.660 IU/mL), and laccase (0.640 and 0.742 U/mL) activities at incubation time 7 days, 37(o)C and initial pH 6.4, using yeast extract as nitrogen source and cheap substrate (wheat bran), which increased the cost effectiveness of the process. Crude cellulase-poor xylanases obtained from test strains showed maximum activities at 55(o)C and pH 6.4 and...
Source: New Biotechnology - September 13, 2009 Category: Biotechnology Authors: Singh S, Tyagi CH, Dutt D, Upadhyaya JS Tags: N Biotechnol Source Type: journals

Effect of Surfactant on Production of Oxygenated Unsaturated Fatty Acids by Bacillus megaterium ALA2.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bacillus megaterium ALA2 NRRL B-21660 has been well studied for production of many oxygenated unsaturated fatty acids from linoleic acid. Its major product, 12,13,17-trihydroxy-9(Z)-octadecenoic acid (12,13,17-THOA), inhibited the growth of many plant pathogenic fungi. However, we have been unable, until now, to demonstrate 12,13,17-THOA production in a fermenter. Here, we have investigated the effect of surfactants on 12,13,17-THOA production. Surfactant types (SO-25, Tween-80 and Triton X-100) at various concentrations were evaluated for their effects on cell growth and production of 12,13,17-THOA. Triton X-100 decre...
Source: New Biotechnology - September 9, 2009 Category: Biotechnology Authors: Kim BS, Kim HR, Hou CT Tags: N Biotechnol Source Type: journals

Isolation of a Methylobacillus sp. that degrades of Microcyctin toxins associated with cyanobacteria.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Sludge from cyanobacteria-salvaged yard in Meiliang Bay, Lake Taihu in Wuxi, China was cultured and acclimated by inoculating microcystins (MCs) extract. Strain J10 was isolated with the ability of degrading the MC-RR and LR, and identified as Methylobacillus sp. Further research showed that both MC-LR and RR could be completely degraded at 17h after inoculation of J10, and the degradation probably was mediated by oxygen. Different enzymes with the MCs-degrading activity were found respectively in different fractions of J10 culture, including the oxygen-dependent and oxygen-independent. However, the enzymes responsible...
Source: New Biotechnology - September 9, 2009 Category: Biotechnology Authors: Hu LB, Yang JD, Zhou W, Yin YF, Chen J, Shi ZQ Tags: N Biotechnol Source Type: journals

Optimization of the enzymatic production of 20(S)-ginsenoside Rg(3) from white ginseng extract using response surface methodology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The optimization of the conversion of ginseng saponin glycosides to 20(S)-ginsenoside Rg(3) by enzymatic transformation was carried out using response surface methodology (RSM) based on a 2(3) factorial central composite design. The production of 20(S)-ginsenoside Rg(3) using several commercial enzymes indicated that the enzyme Cellulase-12T was the most efficient at producing 20(S)-ginsenoside Rg(3). To optimize the enzymatic production of 20(S)-ginsenoside Rg(3), response surface methodology was applied to determine the ideal amount of white ginseng extract, Cellulase-12T and reaction time. These results indicate tha...
Source: New Biotechnology - September 3, 2009 Category: Biotechnology Authors: Chang KH, Jee HS, Lee NK, Park SH, Lee NW, Paik HD Tags: N Biotechnol Source Type: journals

Control of plant diseases with secondary metabolite of Clitocybe nuda.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Culture filtrates of five strains of Clitocybe nuda displayed various degrees of antimicrobial activity against plant pathogenic fungi and/or bacteria tested. The culture filtrate of C. nuda strain LA82 was very effective in reducing the incidence of Phytophthora blight of pepper caused by Phytophthora capsici and the incidence of leaf spot on pepper caused by Xanthomonas axonopodis pv. vesicatoria. The inhibitory substance in the C. nuda strain LA82 culture filtrate was stable at low and high pH. It was also stable at high temperature. The inhibitory substance was dialyzable in the membrane tubing with molecular weigh...
Source: New Biotechnology - September 3, 2009 Category: Biotechnology Authors: Chen JT, Huang JW Tags: N Biotechnol Source Type: journals

Specific sequence selection and next generation re-sequencing of 68 E. coli genes using HybSelect(TM).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present here an in-depth study of HybSelect, a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle. PMID: 19735750 [PubMed - as supplied by publisher] (Source: New Biotechnology)
Source: New Biotechnology - September 3, 2009 Category: Biotechnology Authors: Schracke N, Kornmeyer T, Kränzle M, Stähler PF, Summerer D, Beier M Tags: N Biotechnol Source Type: journals

Detection of Protein-Protein Interactions by Ribosome Display and Protein In Situ Immobilisation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a method for identification of protein-protein interactions by combining two cell-free protein technologies, namely ribosome display and protein in situ immobilisation. The method requires only PCR fragments as the starting material, the target proteins being made through cell-free protein synthesis, either associated with their encoding mRNA as ribosome complexes or immobilised on a solid surface. The use of ribosome complexes allows identification of interacting protein partners from their attached coding mRNA. To demonstrate the procedures, we have employed the lymphocyte signalling proteins Vav1 and Grb2 an...
Source: New Biotechnology - August 27, 2009 Category: Biotechnology Authors: He M, Liu H, Turner M, Taussig MJ Tags: N Biotechnol Source Type: journals

Factors affecting protoplast formation by Rhizoctonia solani.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Novozym 234 was the most frequently used enzyme for production of Rhizoctonia solani protoplasts. Since manufacture of this enzyme was discontinued in the late 1990s, a new procedure was developed by testing lytic enzymes from Sigma and by examining factors affecting protoplast formation. The combination of 20mg/mL Driselase and 10mg/mL Lysing enzyme was effective in releasing protoplasts from R. solani. The optimal condition for enzyme treatment of mycelium was incubation at 37C for 15min followed by 34C for 105min. The amount of protoplasts produced was positively correlated with growth rate and negatively correlated...
Source: New Biotechnology - August 24, 2009 Category: Biotechnology Authors: Liu TH, Lin MJ, Ko WH Tags: N Biotechnol Source Type: journals

The Saccharomyces cerevisiaeYMR315W gene encodes an NADP(H) specific oxidoreductase regulated by the transcription factor Stb5p in response to NADPH limitation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we purified histidine-tagged enzyme and determined that Ymr315wp is an NADP(H) specific oxidoreductase. We also showed that YMR315W transcription is regulated by Stb5p in response to diamide induced NADPH depletion. Overexpression of Ymr315wp in BY4727 cells resulted in elevated NADPH levels and increased resistance to diamide. However, the presence of Ymr315wp in cells lacking the oxidative branch of the pentose phosphate pathway resulted in decreased NADPH levels and increased diamide sensitivity. These results suggest that in BY4727 cells Ymr315wp contributes to NADPH production as an alternative source o...
Source: New Biotechnology - August 23, 2009 Category: Biotechnology Authors: Hector RE, Bowman MJ, Skory CD, Cotta MA Tags: N Biotechnol Source Type: journals

Influences of Different Stress Media and High Light Intensities on Accumulation of Astaxanthin in the Green Alga Haematococcus pluvialis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Haematococcus pluvialis Flotow is used in the aquaculture, pharmaceutical and cosmetic industries. The aim of this study was to compare the effect of various stress media and high light intensities on astaxanthin accumulation. The experimental design was achieved by four different stress media and two different light intensities for 14 days of induction period. The astaxanthin concentrations of 29.62mg g(-1) and 30.07mg g(-1) were obtained in distilled water with CO(2) and N-free medium, respectively, with no significant difference between them at 546mumol photons m(-2) s(-1). Because of the morphological changes of H....
Source: New Biotechnology - August 23, 2009 Category: Biotechnology Authors: Imamoglu E, Dalay MC, Sukan FV Tags: N Biotechnol Source Type: journals

Antiviral activity of ethanol extracts of Ficus binjamina and Lilium candidum in vitro.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The antiviral activity of plant ethanol extracts against Herpes Simplex Virus -1 and 2 (HSV-1, HSV-2) and Varicella-Zoster Virus (VZV) was investigated in vitro. Ficus binjamina, resistant to plant viruses, and Lilium candidum, which has a high susceptibility to plant viruses were used. Leaf extracts of F. binjamina inhibited all studied viruses, while its fruit extracts inhibited only VZV. L. candidum leaf extracts had no effect on VZV but strongly inhibited HSV-1 and slightly HSV-2. None of the extracts showed significant cytotoxic effect on uninfected Vero cells even at a concentration of 250mug/ml (CC(50)>400mug...
Source: New Biotechnology - August 20, 2009 Category: Biotechnology Authors: Yarmolinsky L, Zaccai M, Ben-Shabat S, Mills D, Huleihel M Tags: N Biotechnol Source Type: journals

Activity of immobilised rat hepatic microsomal CYP2E1 using alumina membrane as a support.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Porous alumina membranes are attractive materials for the construction of biosensors and also have utility for the production of immobilised enzyme bioreactors. Microsomes from rat liver were adsorbed onto alumina membrane activated by silane. Microsomal membranes were pumped through the channels where they became immobilised by binding to amine groups on the surface of the alumina membrane. In an effort to gain a quantitative understanding of the effects of microsomal film growth on enzyme activity, we compared the para-nitrophenol (pNP) hydroxylase activity of the microsomes by varying the amount of microsomes fixed ...
Source: New Biotechnology - August 20, 2009 Category: Biotechnology Authors: Tanvir S, Morandat S, Frederic N, Adenier H, Pulvin S Tags: N Biotechnol Source Type: journals

Gene Transfection of HEK Cells on Supermacroporous Polyacrylamide Monoliths: A comparison of transient and stable recombinant protein expression in perfusion culture.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transient and continuous recombinant protein expression by HEK cells was evaluated in a perfused monolithic bioreactor. Highly porous synthetic cryogel scaffolds (10ml bed volume) were characterised by scanning electron microscopy and tested as cell substrates. Efficient seeding was achieved (94% inoculum retained, with 91-95% viability). Metabolite monitoring indicated continuous cell growth, and endpoint cell density was estimated by genomic DNA quantification to be 5.2x10(8), 1.1x10(9) and 3.5x10(10) at day 10, 14 and 18. Culture of stably transfected cells allowed continuous production of the Drosophila cytokine Sp...
Source: New Biotechnology - August 20, 2009 Category: Biotechnology Authors: Cheeks MC, Edwards AD, Arnot CJ, Slater NK Tags: N Biotechnol Source Type: journals

Company profile: Hexascreen Culture Technologies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19682611 [PubMed - as supplied by publisher] (Source: New Biotechnology)
Source: New Biotechnology - August 10, 2009 Category: Biotechnology Authors: Astals AS Tags: N Biotechnol Source Type: journals

Preface.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19679204 [PubMed - as supplied by publisher] (Source: New Biotechnology)
Source: New Biotechnology - August 9, 2009 Category: Biotechnology Authors: Hou CT Tags: N Biotechnol Source Type: journals

Cultivation and bioactivity assessment of Grifola frondosa fruiting bodies on olive oil press cakes substrates.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Grifola frondosa, a medicinal and edible mushroom, was cultivated on substrates composed of olive oil press cakes with different supplements. Crude extracts were prepared from mature fruiting bodies and tested for their capacity to stimulate splenocyte proliferation. Two wild-growing fruiting bodies were extracted for comparison. Olive oil press cakes reduced the mushroom yield and the best biological efficiency was obtained on substrates supplemented with wheat bran and without olive oil press cakes. All extracts were capable of inducing splenocyte proliferation and were half as effective as the positive control (6.0 ...
Source: New Biotechnology - August 6, 2009 Category: Biotechnology Authors: Gregori A, Svagelj M, Berovic M, Liu Y, Zhang J, Pohleven F, Klinar D Tags: N Biotechnol Source Type: journals

Yeast extract mediated auto-induction of lacUV5 promoter: An insight.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a simple and cost-effective auto inducible media component responsible for auto induction of proteins in E. coli under lacUV5 promoter system. Yeast extract at high concentration was found to stimulate the expression of T7 RNA polymerase in BL21 (DE3) cells while such an effect was not seen in BL21A1 cells. A systematic study on the effect of varying concentrations of yeast extract indicated several folds higher expression of genes viz., human granulocyte colony stimulating factor (rhGCSF), human interferon alpha 2b (rhIFN-alpha2b) and Staphylokinase (rSAK) in BL21(DE3) cells in absence of any specific inducer li...
Source: New Biotechnology - August 6, 2009 Category: Biotechnology Authors: Nair R, Salvi P, Banerjee S, Raiker VA, Bandyopadhyay S, Soorapaneni S, Kotwal P, Padmanabhan S Tags: N Biotechnol Source Type: journals

Magnetic bead-based detection of autoimmune responses using protein microarrays.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study, a magnetic bead-based detection approach for protein microarrays is described as an alternative approach to the commonly used fluorescence-based detection system. Using the bead-based detection approach with applied magnetic force, it was possible to perform the detection step more rapidly as a result of the accelerated binding between the captured analyte in the microspot and the detection antibody, which was coupled to the magnetic beads. The resulting strong opacity shift on the microspots could be recorded with an ordinary flatbed scanner. In the context of autoimmunity, a set of 24 serum samp...
Source: New Biotechnology - August 3, 2009 Category: Biotechnology Authors: Gantelius J, Hartmann M, Schwenk JM, Roeraade J, Andersson-Svahn H, Joos TO Tags: N Biotechnol Source Type: journals

Optimal production of a novel endo-acting beta-1,4-xylanase cloned from Saccharophagus degradans 2-40 into Escherichia coli BL21 (DE3).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To date, gene xyn10C from Saccharophagus degradans 2-40, has only been identified to encode a potential xylanase. In the present study, xyn10C was cloned and over-expressed in Escherichia coli BL21 (DE3). The protein produced by xyn10C, Xyn10C, was expressed in a soluble active form and found to be an endo-type beta-1,4-xylanase that preferentially produces xylobiose from xylan. Recombinant cell fermentation revealed that induction of the gene at low temperatures fostered expression of the recombinant xylanase with high volumetric and specific activities. Additionally, low growth rates were favorable for producing solu...
Source: New Biotechnology - July 27, 2009 Category: Biotechnology Authors: Ko JK, Jung MW, Kim KH, Choi IG Tags: N Biotechnol Source Type: journals

Optimization of Lipase-catalyzed Biodiesel by Isopropanolysis in a Continuous Packed-bed Reactor Using Response Surface Methodology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study modeled the degree of isopropanolysis of soybean oil in a continuous packed-bed reactor when Novozym((R)) 435 was used as the biocatalyst. Response surface methodology (RSM) and 3-factor-3-level Box-Behnken design were employed to evaluate the effects of synthesis parameters, reaction temperature ( degrees C), flow rate (mL/min), and substrate molar ratio of isopropanol to soybean oil, on the percentage molar conversion of biodiesel by transesterification. The results show that flow rate and temperature have a significant effect on the percentage of molar conversion. Based on ridge max analysis, the optimum cond...
Source: New Biotechnology - July 27, 2009 Category: Biotechnology Authors: Chang C, Chen JH, Wu TT, Shieh CJ Tags: N Biotechnol Source Type: journals

Bioconverted products of essential fatty acids as potential antimicrobial agents.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This review deals with the recent findings on the microbial conversion of essential fatty acids (EFAs) through Pseudomonas aeruginosa PR3 NRRL-B-18602, and the antimicrobial properties of bioconverted essential fatty acids, with particular emphasis on n-3 or n-6 fatty acids. The first section deals with the most recent and some background literature on what have been the latest developments with respect to bioconverted essential fatty acids as potential antibacterial agents, in particular the inhibition of severe foodborne and food spoilage bacteria causing deleterious effects in food and human beings. The second secti...
Source: New Biotechnology - July 26, 2009 Category: Biotechnology Authors: Bajpai VK, Kim HR, Hou CT, Kang SC Tags: N Biotechnol Source Type: journals

Production and distribution of beta-glucosidase in a mutant strain Trichoderma viride T 100-14.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The characterization of beta-glucosidase's production and distribution in a mutant strain Trichoderma viride T 100-14 at extracellular and intracellular levels were studied in this paper. Three experiment groups were done with pH controlled at 4.8 automatically during fermentation process, with 1mg/ml 2-deoxy-D-glucose addition or without pH control and 2-deoxy-D-glucose addition (control). Activity assay and electron microscopic immunogold labelling experiments were performed at different culture periods (24, 48, 72, 96 and 120h). Under constant pH 4.8, high density of immunogold labelling particles, highest intracell...
Source: New Biotechnology - July 26, 2009 Category: Biotechnology Authors: Wang YH, Zhou J, Chu J, Zhang SL, Zhuang YP Tags: N Biotechnol Source Type: journals

Brassinosteroid: a biotechnological target for enhancing crop yield and stress tolerance.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Brassinosteroids (BRs) are a group of naturally occurring plant steroidal compounds with wide ranging biological activity. Since BRs control several important agronomic traits such as flowering time, plant architecture, seed yield, and stress tolerance, the genetic manipulation of BR biosynthesis, conversion or perception offers a unique possibility of significantly increasing crop yields through both changing plant metabolism and protecting plants from environmental stresses. Genetic manipulation of BR activity has indeed led to increases in crop yield by 20-60%, confirming the value of further research on BRs to impr...
Source: New Biotechnology - July 21, 2009 Category: Biotechnology Authors: Divi UK, Krishna P Tags: N Biotechnol Source Type: journals

Multimerization domains for antibody phage display and antibody production.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
High throughput generation of antibodies for proteome research has become feasible by using antibody gene libraries and in vitro selection methods like phage display. Typically monovalent antibody fragments like scFv, Fab or scFab are obtained by this technology. To mimic the IgG molecule and gain avidity, resulting in stronger binding, multimerization domains can be fused to antibody fragments. Here we systematically analyzed different multimerization domains in respect of three key parameters, critical for the high throughput generation of binders. (i) The compatibility to be displayed on phage, (assessed for at leas...
Source: New Biotechnology - July 20, 2009 Category: Biotechnology Authors: Thie H, Binius S, Schirrmann T, Hust M, Dübel S Tags: N Biotechnol Source Type: journals

Synthesis of cellobiose from starch by the successive action of phosphorylases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cellobiose was enzymatically synthesized from starch using two phosphorylases. Under the presence 1M Pi (inorganic phosphate), glucan phosphorylase converted 40% of glucose residues in the starch molecule into G1P (glucose-1-phosphate). By electrodialysis fitted with an ion exchange membrane having molecular weight cutoff of 100, Pi was effectively dialyzed out and G1P was recovered with 80% yield. G1P and glucose were incubated with cellobiose phosphorylase in the presence of magnesium acetate at an alkaline condition. Inorganic phosphate co-formed with cellobiose was immediately removed as insoluble magnesium ammoniu...
Source: New Biotechnology - July 20, 2009 Category: Biotechnology Authors: Suzuki M, Kaneda K, Nakai Y, Kitaoka M, Taniguchi H Tags: N Biotechnol Source Type: journals

Biocatalysis for the Production of Carbohydrate Esters.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Carbohydrate fatty acid esters are non-ionic biosurfactants which can be synthesized from esterification of mono- or oligosaccharides by enzymatic catalysis. These esters are increasingly used as important commodity chemicals, such as low calorific sweeteners and biosurfactants in food, pharmaceutical and cosmetic industries. Recently, some of ester derivatives have shown their therapeutic potential with antitumor activity, plant growth inhibition, and antibiotic activities which became one of the "hot" subjects for various biological processes. However, this potential has not been fully explored because the production...
Source: New Biotechnology - July 19, 2009 Category: Biotechnology Authors: Chang SW, Shaw JF Tags: N Biotechnol Source Type: journals

Hottest Topics in Protein Research: MedILS Summer School.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19616653 [PubMed - as supplied by publisher] (Source: New Biotechnology)
Source: New Biotechnology - July 15, 2009 Category: Biotechnology Authors: Blaskovic S, Cretnik M, Juric S, Korac J, Marjanovic M, Matijevic T Tags: N Biotechnol Source Type: journals

Editorial.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19615476 [PubMed - as supplied by publisher] (Source: New Biotechnology)
Source: New Biotechnology - July 14, 2009 Category: Biotechnology Authors: van Montagu M, Uhlen M Tags: N Biotechnol Source Type: journals

Interview with Dr. Heinz-Hubert Fischer, recruitment consultant with Kelly Scientific Resources.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19596468 [PubMed - as supplied by publisher] (Source: New Biotechnology)
Source: New Biotechnology - July 7, 2009 Category: Biotechnology Authors: Ahl V Tags: N Biotechnol Source Type: journals

Life Science Careers: How to make best use of Europe's brains.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19591964 [PubMed - as supplied by publisher] (Source: New Biotechnology)
Source: New Biotechnology - July 6, 2009 Category: Biotechnology Authors: Epstein I Tags: N Biotechnol Source Type: journals

How Microbes Tolerate Ethanol and Butanol.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
New robust biocatalysts are needed to depolymerize or hydrolyze recalcitrant heterogeneous lignocellulosic biomass polymers into monomers and to convert the mixed substrates into biofuels. The ideal biocatalysts should be able to tolerate inhibitory compounds released from biomass hydrolysis and increased concentrations of the final products: ethanol or butanol. The solvent tolerance trait plays an important role in cost-effective recovery processes. Here we provide an overview of the literature of fermenting microbes in response to increased ethanol or butanol concentrations, aimed to provide insight on how microbes d...
Source: New Biotechnology - June 30, 2009 Category: Biotechnology Authors: Liu S, Qureshi N Tags: N Biotechnol Source Type: journals

Selection of TNF-alpha binding affibody molecules using a beta-lactamase protein fragment complementation assay.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Protein fragment complementation assays (PCAs) based on different reporter proteins have been described as powerful tools for monitoring dynamic proteinprotein interactions in living cells. The present study describes the construction of a PCA system based on genetic splitting of TEM-1 beta-lactamase for selection of proteins specifically interacting in the periplasm of Escherichia coli bacterial cells, and its application for selecting affibody molecules binding human tumor necrosis factor-alpha (TNF-alpha) from a combinatorial library. Vectors encoding individual members of a naïve 10(9) affibody protein library...
Source: New Biotechnology - June 29, 2009 Category: Biotechnology Authors: Löfdahl PA, Nord O, Janzon L, Nygren PA Tags: N Biotechnol Source Type: journals