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"Pak J Biol Sci"[ta]; +109 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
109 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Pak J Biol Sci"[ta] These pubmed results were generated on 2009/07/09PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources.
Source: Pakistan Journal of Biological Sciences: PJBS - July 10, 2009 Category: Biomedical Science Tags: Report Source Type: journals

"Pak J Biol Sci"[ta]; +124 new citationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
124 new PubMed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Pak J Biol Sci"[ta] These PubMed results were generated on 2008/12/20PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources.
Source: Pakistan Journal of Biological Sciences: PJBS - December 20, 2008 Category: Biomedical Science Tags: Report Source Type: journals

Effects of copper and superoxide dismutase content of seminal plasma on buffalo semen characteristics.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To investigate the effects of copper and superoxide dismutase (SOD) content of seminal plasma on buffalo semen characteristics, 54 semen samples collected from buffalo bulls by a bovine artificial vagina were used. Semen characteristics (motility, viability, morphology, concentration and volume) were recorded. Seminal plasma was harvested by centrifugation and kept frozen until analysis. Seminal plasma copper content was determined by atomic absorption procedure and SOD was measured by using a kit. The mean total copper value of seminal plasma was recorded as 2.51 +/- 0.04 mg kg(-1) (Mean +/- SEM) and the mean total SO...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Eghbali M, Alavi-Shoushtari SM, Rezaii SA Tags: Pak J Biol Sci Source Type: journals

Ethnobotanical study of plants used for the treatment of diarrhoea in the Eastern Cape, South Africa.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An ethnobotanical study of plants used for the treatment of diarrhoea in the Eastern Cape Province, South Africa was carried out, using a questionnaire which was administered to herbalists, traditional healers and rural dwellers. Information collected revealed the names of plants used for the treatment of diarrhoea, the parts used and the methods of preparation. This survey indicated a total of 17 plant species from 14 families used in the Province. Elephantorrhiza elephantina, Hermannia incana, Pelargonium reniforme, Alepidea amatymbica and Bulbine latifolia were the most frequently mentioned and highly recommended pl...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Appidi JR, Grierson DS, Afolayan AJ Tags: Pak J Biol Sci Source Type: journals

Structural changes in root tips of wheat (Triticum aestivum L.) in response to olive oil mill wastewater.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Toxic effects of the wastewater were investigated ultrastructurally in root tips of Triticum aestivum. As a result, wall and nuclear degradations, disruptions in all cytoplasmic membranes, irregular nucleus shapes and cellular organization defects were densely detected. Besides, germination ratio, total protein contents, DNA contents and root-shoot growth were found to be decreased significantly when compared to the control group. Results were compared with those of recent studies regarding excessive Na+, Fe+2, P, polyphenols and acidic pH toxicity. PMID: 18983041 [PubMed - in process]
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Aybeke M, Sidal U, Hüseym G Tags: Pak J Biol Sci Source Type: journals

Effect of sowing dates on yield and yield components of spring safflower (Carthamus tinctorius L.) in Isfahan region.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to study the effect of sowing dates on the yield and yield components of two safflower varieties, an experiment was conducted at Agriculture Research Station of Isfahan Kabotar Abad in 2004. A split plot layout within randomized complete block design with three replications was used in the experimentation. Eight sowing dates were in the main plots, consist March 6, March 21, April 6, April 21, May 6, May 21, June 6 and June 21 and two varieties (Isfahan 14 and I.L111) were in the sub plots. The results showed that the number of seed per capitulum and seed yield were decreased significantly as the sowings dates...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Nikabadi S, Soleimani A, Dehdashti SM, Yazdanibakhsh M Tags: Pak J Biol Sci Source Type: journals

Effects of different plant density and nitrogen application rate on nitrogen use efficiency of potato tuber.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to investigate the plant density and nitrogen level on nitrogen use efficiency components (agronomical, physiological, apparent recovery and nitrogen use efficiency), the amount of nitrogen uptake by plant, yield and yield components of potato (Solanum tuberosum L.) Agria cultivars' tuber, a factorial experiment based on randomized complete block design was conducted in Ardabil, Iran, in 2006 with three replications. Factors were adjusted for the nitrogen level (0, 80, 160 and 200 kg ha(-1) net nitrogen) and plant density (5.5, 7.5 and 11 plant m(-2)). Results showed that with increasing the nitrogen levels an...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Jamaati-e-Somarin Sh, Tobeh A, Hassanzadeh M, Saeidi M, Gholizadeh A, Zabihi-e-Mahmoodabad R Tags: Pak J Biol Sci Source Type: journals

The use of carbohydrate antigen (CA) 15-3 as a tumor marker in detecting breast cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was carried out to determine the sensitivity and specificity of serum CA 15-3 as a marker in detecting and monitoring treatment in, breast cancer patients. One hundred and ten patients comprising 35 known breast cancer patients, 75 suspected cases and 20 controls entered the study. Blood samples were taken before and after treatment from the 35 known cases as well as the 75 suspected cases from which biopsy specimens were also taken. Serum CA 15-3 was measured by BioCheck CA 15-3 Enzyme Immunoassay. There was a significant difference between the concentration of serum CA 15-3 of the 35 known breast cancer patien...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Agyei Frempong MT, Darko E, Addai BW Tags: Pak J Biol Sci Source Type: journals

Antimicrobial susceptibility pattern of microorganisms involved in the pathogenesis of surgical site infection (SSI); A 1 year of surveillance.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study is to identify the antibiotic sensitivity pattern of pathogens involved in the process of surgical site infection, in surgical wards. Changes made in the pattern of antibiotic use will result in different microorganism susceptibility patterns, which needs correct determination for precise empiric antibiotic therapy. One thousand patients (62% men and 38% women, 18- 74-years-old, with mean age 43 +/- 8)) who underwent surgical treatment, in Alzahra University Hospital, Isfahan University of Medicine, Isfahan, Iran, were studied from 2005 to 2006. Surgical wound infections, based on the reported cri...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Khorvash F, Mostafavizadeh K, Mobasherizadeh S, Behjati M, Naeini AE, Rostami S, Abbasi S, Memarzadeh M, Khorvash FA Tags: Pak J Biol Sci Source Type: journals

Evaluation of bean (Phaseolus vulgaris) seeds inoculation with Rhizobium phaseoli and plant growth promoting rhizobacteria on yield and yield components.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To study the effect of co-inoculation with plant growth-promoting rhizobacteria (PGPR) and Rhizobium, on yield and yield components of common bean (Phaseolus vulgaris L.) cultivars was investigated in 2 consecutive years under field condition of plant growing evidence indicates that soil beneficial bacteria can positively affect symbiotic performance of rhizobia. PGPR strains Pseudomonas fluorescens P-93 and Azospirillum lipoferum S-21 as well as two highly effective Rhizobium strains were used in this study. Common bean seeds of three cultivars were inoculated with Rhizobium singly or in a combination with PGPR to eva...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Yadegari M, Rahmani HA, Noormohammadi G, Ayneband A Tags: Pak J Biol Sci Source Type: journals

A study on the effect of thiotepa on mice spermatogenesis using light and electronic microscope.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study was designed to explore the spermatogenic effect of intraperitoneal administration of thiotepa in mice. It is known the chemotherapy could affect testicular function and result in infertility. There are few reports on the side effect of thiotepa on spermatogenesis when are used as anticancer drug. Therefore, in the present study the effect of thiotepa on spermatogenesis was evaluated by electron microscopy. A total of 20 balb/c mice were used in this study. They were divided into control and thiotepa treated groups. Thiotepa was administrated as 2.5 mg kg(-1) for 5 days. The mice were sacrificed after...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Nejad DM, Rad JS, Roshankar L, Karimipor M, Ghanbari AA, Aazami A, Valilou MR Tags: Pak J Biol Sci Source Type: journals

Genetic diversity of some mediterranean populations of the cultivated alfalfa (Medicago sativa L.) using SSR markers.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This species study was to investigate the differentiation level among 26 populations in which 12 are locals originating from the Tunisian South and 14 introduced from Italy, Austerely, France and Morocco with two SSR markers. These highly polymorphic and co dominant markers, together with recent population genetic statistic extended to autotetraploids, offer tools to analyse genetic diversity in alfalfa. The number of alleles per locus varied between 8 and 9. The genetic similarity between these various populations is estimated by the index of Rogers and Tanimoto. Genetic diversity is analysed by two statistical proced...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Touil L, Guesmi F, Fares K, Zagrouba C, Ferchichi A Tags: Pak J Biol Sci Source Type: journals

The effect of water stress on the antioxidant content, protective enzyme activities, proline content and lipid peroxidation in wheat seedling.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An investigation was conducted to study the effect of water stress on the antioxidant content, protective enzyme activities, proline content and lipid peroxidation in wheat seedlings. Drought stress increases the amount of Reactive Oxygen Species (ROS), leading to metabolic disorders. It is now known that higher levels of activity-protective mechanisms render the cells more enduring against environmental stress including drought. Two widely cultivated cultivars of wheat in Iran, Sab. and N. Sar. were grown up according to the hydroponic method. Having reached the stage of 4-5 leaves growth; the plants were kept under 4...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Esfandiari E, Shakiba MR, Mahboob SA, Alyari H, Shahabivand S Tags: Pak J Biol Sci Source Type: journals

Soil salinity affects arbuscular mycorrhizal colonization of halophytes.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to determine the effects of soil salinity on AM fungi colonization in halophytes, plants of semi-arid region of North-Eastern Iran were examined for their colonization in soils with different salinity levels. Roots of several halophytes were colonized and showed typical structure of AM fungi with different levels of colonization. Haloxylon aphyllum, Kochia stellaris, Halocnemum strobilaceum, Seidlitzia rosmarinus and Salsola sp. of the Chenopodiaceae and Zygophyllum eurypterum and Peganum harmala of the Zygophyllaceae were found to be colonized by AM fungi. In several species the mycorrhizal status is reported...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Asghari HR, Amerian MR, Gorbani H Tags: Pak J Biol Sci Source Type: journals

Morphological alteration in mitochondria following diclofenac and ibuprofen administration.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was conducted to identify and to compare the mitochondrial morphological alterations in livers of rats treated with various doses of diclofenac and ibuprofen. Hundred and forty-four male Sprague Dawley rats were dosed with 3, 5 and 10 mg kg(-1) diclofenac and ibuprofen in saline via intraperitoneal injection for 15 days. The control group was administered with saline in a similar manner. Four rats were euthanised every 3 days until day 15. While 200 mg kg(-1) diclofenac and ibuprofen-treated rats (n = 4) were euthanized 10 h posttreatment. The livers were removed, cleaned and a section across the right lobe was ...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Moorthy M, Fakurazi S, Ithnin H Tags: Pak J Biol Sci Source Type: journals

Application of IRS-1D data in water erosion features detection (case study: Nour roud catchment, Iran).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, ability of PAN digital data of IRS-1D satellite was evaluated for extraction of erosion features in Nour-roud catchment located in Mazandaran province, Iran, using GIS techniques. Research method has based on supervised digital classification, using MLC algorithm and also visual interpretation, using PMU analysis and then these were evaluated and compared. Results indicated that opposite of digital classification, with overall accuracy 40.02% and kappa coefficient 31.35%, due to low spectral resolution; visual interpretation and classification, due to high spatial resolution (5.8 m), prepared classifying ero...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Solaimani K, Amri MA Tags: Pak J Biol Sci Source Type: journals

Maleo- and fumaro-pimaric acids synthesized from Indonesian Pinus merkusii rosin and their sizing properties.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This research is directed at characterizing purified maleopimaric acid (MPA), looking at the amount of MPA and fumaropimaric acid (FPA) made from a large amount of rosin and maleic anhydride (MA) or fumaric acid (FA) with various molar ratios and sources of rosin and evaluating the properties of fortified rosin sizes made from both MPA and FPA. Results showed that identification of the Diels-Alder adduct of abietic acid and maleic anhydride using Mass spectrometry produced a mixture of endo-maleopimaric acid methyl ester with endo-maleopimaric acid tri methyl ester, as indicated by a fragment ion at m/z = 146 with a mo...
Source: Pakistan Journal of Biological Sciences: PJBS - August 1, 2008 Category: Biomedical Science Authors: Wiyono B, Tachibana S Tags: Pak J Biol Sci Source Type: journals

Evaluation of effect of PxGV-Taiwanii on cabbage moth Plutella xylostella (Lep.: Plutellidae) in laboratory conditions.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The effect of one entomopathogenic virus (PxGV) was studied on cabbage moth Plutella xylostella with hope to find management strategies of this insect, based on biological control. Bioassay showed that, this virus has high virulence and can be considered as the important agents on the control of this insect. The LC50 value of PxGV for second instar larvae of cabbage moth was calculated 448.58 g mm(-2). The LT50 values for the same larvae with 749.89 and 1883.65 g mm(-2) doses of PxGV were 6.04 and 6.85 days, respectively. PMID: 18819637 [PubMed - indexed for MEDLINE]
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Fahimi A, Kharrazi-Pakdel A, Talaei-Hassanloui R Tags: Pak J Biol Sci Source Type: journals

Cholestatic syndromes of infancy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aimed to provide the analysis of clinical presentation, results of laboratory and imaging investigations as well as clinical outcome of children with cholestasis. Infants with neonatal cholestasis referred to Children's Hospital from 2002 to 2007 were participated in the study in a cross-sectional prospective study. Appropriate diagnostic criteria and tests were employed for diagnosis the underlying etiologies of neonatal cholestasis. One year mortality rate was determined. One hundred twenty one infants, 75 males and 46 females, with the mean age of 58.3 +/- 15.3 (14-120) days were enrolled in study. Jaundice (...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Rafeey M, Golzar A, Javadzadeh A Tags: Pak J Biol Sci Source Type: journals

Urografin in the treatment of sudden sensorineural hearing loss.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study revealed no significant difference in hearing improvement between the hospitalized patients who received intravenous urografin and corticosteroid and the patients who received oral corticosteroid alone. PMID: 18819635 [PubMed - indexed for MEDLINE]
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Totonchi JS, Nejadkazem M, Ghabili K, Ayat SE, Rad SR Tags: Pak J Biol Sci Source Type: journals

The first host record for Scambus sagax (Hartig, 1838) (Hymenoptera: Ichneumonidae) from Turkey.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Scambus sagax (Hartig) (Hymenoptera: Ichneumonidae) was reared from larvae of Agapanthia osmanlis Reiche et Saulcy (Coleoptera: Cerambycidae) in the Bayburt province (Kop Mountain Pass). Agapanthia osmanlis is the first known host for Scambus sagax in Turkey. PMID: 18819634 [PubMed - indexed for MEDLINE]
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Coruh S, Tozlu G Tags: Pak J Biol Sci Source Type: journals

Evaluation of clinical examination for differential diagnosis of lameness by navicular apparatus or heel pain in horses.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
To evaluation of clinical examination for differential diagnosis of navicular region pain from other forms of palmar heel pain in the forelimb in horses the present study was undertaken. Thirty four horses with lameness referable to the palmar aspect of the hoof based on their response to the palmar digital nerves analgesia were divided into 2 groups based on their response to both distal interphalangeal joint and navicular bursa analgesia. Horses that were profoundly improved by both analgesic blocks (distal interphalangeal joint and navicular bursa) were considered to have navicular region pain whereas, all other hor...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Sardari K, Kazemi H Tags: Pak J Biol Sci Source Type: journals

Time of initial detection of fetal structures and anatomic differentiation by using B-mode ultrasound examination in bitches.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ultrasonographic examinations of 10 pregnant bitches with known breeding dates were made on successive days to establish the identifiable characteristics of pregnancy. Subsequent serial examinations were made to sonographically characterize normal canine prenatal development based about the first mating. The initial detection of the fetal and extra-fetal structures were as follows: gestational sac at day 17.88 +/- 1.13 (16-21); zonary placenta in the uterine wall at day 23.70 +/- 0.78 (23-25); embryo initial detection at day 22.80 +/- 1.03 (21-24); heartbeat at day 23.0 +/- 0.94 (22-24); yolk sac membrane at day 24.8 +...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Aissi A, Slimani C Tags: Pak J Biol Sci Source Type: journals

The favorite dosage of garlic meal as a feed additive in broiler chickens ratios.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This experiment carried out as a completely randomized design with six treatments and 3 replicates pens of 100 birds each (total of 1800 birds). Treatments were consisted of a common corn-soybean meal based starter and grower diets supplemented by 0, 0.125, 0.25, 0.5, 1 or 2% garlic meal. At 1-21 days of age, daily feed intake per chicks influences by dietary garlic meal level so that the birds fed diets with 0.125 and 0.25% garlic meal had a significantly higher daily feed intake (p < 0.05) than birds fed 0.5% dietary garlic meal. At 22-42 days of age and the whole experimental period (1-42 days of age), garlic mea...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Javandel F, Navidshad B, Seifdavati J, Pourrahimi GH, Baniyaghoub S Tags: Pak J Biol Sci Source Type: journals

Effect of oral administration of fenitrothion on biochemical and hematological parameters in rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to evaluate the effects of acute exposure to various doses of fenitrothion (FNT) on level of serum glucose, total protein, triglycerides (TG), total cholesterol (TC) and some hematological parameters. The study was conducted on 8-week-old male Wistar rats that divided into four groups (three experimental groups and one control group), were treated orally with different doses (25, 50 and 100 mg kg(-1)) of fenitrothion for 28 consecutive day. After treatment, blood samples were collected for biochemical and hematological studies. Present results demonstrated that exposed groups led to significan...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Afshar S, Heidari R, Farshid AA, Ilkhanipour M Tags: Pak J Biol Sci Source Type: journals

No detected hepatitis B virus-DNA in thalassemic patients infected by hepatitis C virus in Kerman province of Iran.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This research was aimed to investigate the prevalence and clinical impact of occult HBV infection in thalassemic patients with chronic HCV infection. In this cross-sectional study we have totally examined 60 patients suffering HBV and HCV infections by PCR and RT-PCR methods, respectively, in Kerman province of Iran. ELISA technique (RADIM, Italy) was used to detect anti-HBc, anti-HBs and HBsAg. The serum level of liver enzymes (SGOT, SGPT, DB, TB and ALK) were analyzed in the HCV infected patients (MAN, IRAN). Statistical analyses performed using t-test and Chi-square. We found that 27 cases (out of 60) were infected ...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Arababadi MK, Hassanshahi G, Yousefi H, Zarandi ER, Moradi M, Mahmoodi M Tags: Pak J Biol Sci Source Type: journals

Impacts of overgrazing in a long term traditional grazing ecosystem on vegetation around watering points in a semi-arid rangeland of North-Eastern Iran.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, the effects of livestock overgrazing on vegetation of the critical area around livestock watering points in a traditional grazing ecosystem was investigated on a semi-arid rangeland in North-East of Iran. Transects were placed in 8 geographical directions around watering points and samples were taken systematically. In each experimental plot, 4 m2, percentage of canopy cover, abundance, density and species richness of vegetation were calculated. The results of this study showed that the percentage of canopy cover, density and species richness were changed with distance from watering points. Critical area imm...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Hassani N, Asghari HR, Frid AS, Nurberdief M Tags: Pak J Biol Sci Source Type: journals

Nested PCR for the rapid detection of TB from pleural fluid at HUKM Malaysia.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of the present study is rapid detection of tuberculosis from pleural effusion of suspected patients. Molecular technique Nested Polymerase Chain Reaction (PCR) was used for the purpose. A total of 67 pleural fluid collected at Hospital University Kebangsaan Malaysia during May 2005 to October 2006 were sent to Microbiology Laboratory enrolled in the study. Detection rate of Mycobacterium tuberculosis in pleural effusion was 0% by acid-fast bacilli (AFB) staining and 1.5% by culture on Lowenstein-Jensen medium. Mycobacterium tuberculosis was detected by PCR in 9% of the cases. PCR of pleural fluid had 19% sensit...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Salleh SA, Hussin S, Rahman MM Tags: Pak J Biol Sci Source Type: journals

The effects of methanolic extracts of ginger (Zingiber officinale) on human sperm parameters; an in vitro study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was conducted on Zingiber officinale or ginger on motility, grading and morphological aspects of human sperm. Thirty human semen samples were obtained from the local hospital. The samples were swim up by Ham's F10. The samples (0.9 mL) were treated by 0.1 mL of ginger methanolic extracts (0.1, 0.2, 0.4 and 0.6% concentration). Sperm motility, grading and morphology parameters were assessed using light microscope at 0, 2, 4, 8 and 24 h after treatment. Dose and time-dependent decreases in motility accompanied by concomitant decrease in grading 3 and 4 were noticed. Morphologic profiles of the sperms were changed ...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Jorsaraei SG, Yousefnia YR, Zainalzadeh M, Moghadamnia AA, Beiky AA, Damavandi MR Tags: Pak J Biol Sci Source Type: journals

Controlled expression of cholera toxin B subunit from Vibrio cholerae in Escherichia coli.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The ctxB gene, the causative agent of cholera epidemic was successfully cloned from V. cholerae in E. coli. The insertion of the gene was confirmed by PCR as well as restriction digestion analyses. The sequencing results for the gene confirmed that the insert was in the correct orientation and in-frame with the P(BAD) promoter and it showed that the gene was 99% homologous to the published ctxB sequence. The CTB protein was successfully expressed in E. coli using the pBAD/His vector system. The expected protein of approximately 14 kDa was detected by SDS-PAGE and Western blot. The use of pBAD/His vector to express the ...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Haryanti T, Mariana NS, Latifah SY, Yusoff K, Raha AR Tags: Pak J Biol Sci Source Type: journals

Foliar micromorphology of Felicia muricata Thunb., A South African medicinal plant.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The foliar micromorphology of Felicia muricata (Thunb.) Nees (Asteraceae) was observed with the JEOL (JSM-6390LV) Scanning Electron Microscope (SEM). Both the abaxial and adaxial surfaces were characterized by anisocytic stomata which were more prevalent on the abaxial surface than the adaxial surface. The leaves have only one type of multicellular non-glandular trichomes that are long and cylindrical, tapering to a sharp point and running parallel to the leaf surface in the direction of the apices. Crystal deposits were also observed on the surfaces of the leaves near the stomata. Energy dispersive X-ray spectroscopy-...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Ashafa AO, Grierson DS, Afolayan AJ Tags: Pak J Biol Sci Source Type: journals

Growth enhancement of effective microorganisms for bioremediation of crude oil contaminated waters.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The bioremediation of polluted groundwater, wastewater aeration pond and biopond sites was investigated using bacteria isolated from these sites located at the oil refinery Terengganu Malaysia. Out of 62 isolates, only 16 isolates from groundwater (8) and wastewater aeration pond (3) and biopond (5) were chosen based on growth medium containing 1% (v/v) Tapis crude oil. Only four isolates; Acinetobacter faecalis, Staphylococcus sp., Pseudomonas putida and Neisseria elongata showed percentage biodegradation of crude oil more than 50% after 5 days using Mineral Salts Medium (MSM). The effect of physical parameters (tempe...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Mukred AM, Abd-Hamid A, Hamzah A, Yusoff WM Tags: Pak J Biol Sci Source Type: journals

Vitamin A status of pregnant women in Calabar metropolis, Nigeria.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The vitamin A status of 101 pregnant women attending clinic at the University of Calabar Teaching Hospital (UCTH), Calabar, Nigeria was assessed based on 24 h dietary recall, serum retinol concentration, history of night blindness, physical appearance and clinical eye signs. The study revealed that the mean dietary vitamin A intake of the respondents (2645.31 +/- 188.91 microg RE) and their mean serum retinol concentration (31.18 +/- 2.94 microg dL(-1)) were significantly (p < 0.05) higher than the FAO/WHO recommended intake and cutoff level for VAD. No case of night blindness, physical signs and symptoms, or clinic...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Williams IO, Eka OU, Essien EU Tags: Pak J Biol Sci Source Type: journals

Preventive effects of flaxseed and sesame oil on bone loss in ovariectomized rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our results are encouraging because they suggest that PUFAs intake may help to prevent osteoporosis associated with estrogens deficiency. However, further studies are needed to determine the mechanism by which a diet rich in n-3 or lignans modulate bone tissue. PMID: 18819621 [PubMed - indexed for MEDLINE]
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Boulbaroud S, Mesfioui A, Arfaoui A, Ouichou A, el-Hessni A Tags: Pak J Biol Sci Source Type: journals

Evaluation the effects of dextromethorphan and midazolam on morphine induced tolerance and dependence in mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The main aim of this study was to evaluate the effects of dextromethorphan and midazolam and their combination on morphine tolerance and dependence in mice. In the present study, different groups of mice were rendered randomly and received morphine (50 mg kg(-1), s.c.), morphine (50 mg kg(-1), s.c.) + Dextromethorphan (25, 50 and 75 mg kg(-1), i.p.), morphine (50 mg kg(-1), s.c.) + midazolam (0.5, 1 and 2 mg kg(-1), i.p.), morphine (50 mg kg(-1), s.c.) + [Dextromethorphan (25 mg kg(-1), i.p. ) + midazolam (0.5 mg kg(-1), i.p.)] once a day for four days. Tolerance was assessed by administration of morphine (9 mg kg(-1),...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Asl BH, Hassanzadeh K, Khezri E, Mohammadi S Tags: Pak J Biol Sci Source Type: journals

Comparative study of the effects of gentamicin, neomycin, streptomycin and ofloxacin antibiotics on sperm parameters and testis apoptosis in rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to investigate the comparative effects of aminoglycosides and fluoroquinolones on testis apoptosis and sperm parameters in rats. Fifty male Wistar rats were randomly divided into control (n = 10) and experimental (n = 40) groups. The experimental groups subdivided into four groups often. Each received 5 mg kg(-1) (IP) gentamicin, 50 mg kg(-1) (IP) neomycin, 40 mg kg(-1) (IP) streptomycin and 72 mg kg(-1) (IP) ofloxacin daily for 14 days, respectively; however, the control group just received vehicle (IP). In the fourteenth day, rats were killed and sperm analyzed for sperm parameters. Testis t...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Khaki A, Novin MG, Khaki AA, Nouri M, Sanati E, Nikmanesh M Tags: Pak J Biol Sci Source Type: journals

Contributions to the moss flora of Artvin region (Hatila Valley National Park-Turkey).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Field studies were organized for exploring the moss flora of Hatila Valley National Park of Artvin (Turkey) during spring-summer period in 2005-2006. The taxonomic survey yielded eighty-five moss taxa o(comprises of seventy-nine species, six varieties) belonging to 44 genera of 16 families in Hatila Valley National Park of Artvin, Turkey. Bryum rubens Milt., Dicranodontium uncinatufm (Harv.) A. Jaeger., Eurhynchium hians var. rigidum (Boul.) Düll., Hypnum jutlandicum Holmen and Warncke, Rhynchostegiella jacquinii (Garov.) Limpr. and Pseudocrossidium hornschuchianum (Schultz) R. H. Z ander are new records for A4 gr...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Batan N, Ozdemir T Tags: Pak J Biol Sci Source Type: journals

Effects of monensin and increasing crude protein in early lactation on performance of dairy cows.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Twenty-four Holstein dairy cows were used to evaluate the singular and combined effects of different level of crude protein and monensin treatments during the early lactation on digestion and milk yield of dairy cows. The experiment was designed as completely randomized with a 3x2 factorial arrangement of treatments. The factors were three Concentrations of CP supplement (19.5, 21.4 and 23.4% of dry matter) and two levels of monensin (0 and 350 mg per cow per day). This experiment consist of three periods and each period was 3 week in length. Monensin did not affect DMI, milk yield, lactose and SNF but it reduced milk ...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Ghorbani B, Ghoorchi T, Amanlou H, Zerehdaran S Tags: Pak J Biol Sci Source Type: journals

Genetic diversity of storage proteins in cultivated buckwheat.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Prolamin and albumn variations of the storage proteins in 76 cultivated buckwheat accessions (55 accessions of Fagopyrum tataricum, 21 accessions of F. esculentum) from 7 countries were characterized by A-PAGE and SDS-PAGE, respectively, for the purpose of evaluating the genetic diversity of cultivated buckwheat at the level of proteins. A total of 18 prolamin bands were detected, among which 88.89 % bands were polymorphic. The number of albumn bands based on SDS-PAGE observed in accessions ranged from 4 to 10. Most intense bands were in the range of molecular weights from 29 to 97.2 kDa. The average of genetic similar...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Yang YX, Wu W, Zheng YL, Yang WT, Cai QR Tags: Pak J Biol Sci Source Type: journals

An analysis on flavonoids, phenolics and organic acids contents in brewed red wines of both non-skin contact and skin contact fermentation techniques of Mao Luang ripe fruits (Antidesma bunius) harvested from Phupan Valley in Northeast Thailand.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The experiment was carried out at the Department of Biotechnology, Faculty of Technology, Mahasarakham University, Northeast Thailand during the 2006. The study aimed to determine amounts of flavonoids, phenolics and organic acids in ripe fruits and brewed red wines of both non-skin contact and skin contact winemaking techniques where Mao Luang ripe fruits of both Fapratan and Sangkrow2 cultivars were used. The experiment was laid in a Completely Randomised Design (CRD) with four replications. The results showed that mean values of primary data of fresh Mao Luang ripe fruits on weight of 100 berries (g) and mean values...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Samappito S, Butkhup L Tags: Pak J Biol Sci Source Type: journals

Leeching in the history--a review.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to explore the possibility of revival of the leech therapy (leeching) which is still being used traditionally as therapeutic agent in various ailments. Leeching is not the outcome of the medieval period but has been in use during the times when there was no concept of the disease and medicament. The earliest clearly documented record of leeches being used for remedial purpose appears in a painting in an Egyptian Tomb of around 1500 BC. The journey of the leech therapy reached its zenith in 17th and 18th century AD in Europe while as during the Arab era the leeches were used medicinally but only for ...
Source: Pakistan Journal of Biological Sciences: PJBS - July 1, 2008 Category: Biomedical Science Authors: Munshi Y, Ara I, Rafique H, Ahmad Z Tags: Pak J Biol Sci Source Type: journals

Antioxidant status of bilirubin and uric acid in patients diagnosed with Plasmodium falciparum malaria in Douala.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oxidative stress and changes in antioxidant status have been implicated in the pathogenesis of malaria. To assess the antioxidant level ofbilirubin and uric acid associated with falciparum malaria infection, 60 untreated patients (30 men and 30 women) in Douala, Cameroon were screened for the study. Sixty five healthy individuals (29 men and 36 women) were used as controls. Total and conjugated bilirubin were calculated using Jendrassik-Grof method while uric acid was determined using Barham-Trinder method. It was observed that total and conjugated bilirubins were significantly (p < 0.001) higher in malaria patients...
Source: Pakistan Journal of Biological Sciences: PJBS - June 15, 2008 Category: Biomedical Science Authors: Bertrand KE, Mathieu N, Inocent G, Honore FK Tags: Pak J Biol Sci Source Type: journals

Molecular detection of potato spindle tuber viroid in Razavi and Northern Khorasan provinces.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aim of this study was to identify the Potato Spindle Tuber Viroid (PSTVd) by Reverse Transcriptase-PCR. Among all plant diseases which are caused by viroids, PSTVd was the first viroid which is recognized by plant pathologist. Suspended potato tubers from Razavi and Northern Khorasan provinces collected and cultured. Leaves of cultured tubers were used for RNA extraction by PEG6000 Precipitation method. Subsequent RT-PCR reaction has been carried out using specific primers. A 359 bp fragment has been appeared after electrophoresis which has not appeared in healthy tuber samples. Digestion of obtained fragment with ...
Source: Pakistan Journal of Biological Sciences: PJBS - June 15, 2008 Category: Biomedical Science Authors: Arezou Y, Jafarpour B, Rastegar MF, Javadmanesh A Tags: Pak J Biol Sci Source Type: journals

The influence of different hormone concentration and combination on callus induction and regeneration of Rauwolfia serpentina L. Benth.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L(-1) BA and 2.0 mg L(-1) NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L(-1) BA with 0.2 mg L(-1) NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L(-1) each of IBA and IAA. PMID: 18819656 [PubMed - indexed for MEDLINE]
Source: Pakistan Journal of Biological Sciences: PJBS - June 15, 2008 Category: Biomedical Science Authors: Salma U, Rahman MS, Islam S, Haque N, Jubair TA, Haque AK, Mukti IJ Tags: Pak J Biol Sci Source Type: journals

Bioremediation of engine oil polluted soil by the tropical white rot fungus, Lentinus squarrosulus Mont. (Singer).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was conducted to test the efficacy of an indigenous white rot fungus Lentinus squarrosulus in degrading engine oil in soil. Flasks containing sterilized garden soil (100 g) moistened with 75% distilled water (w/v) were contaminated with engine oil 1, 2.5, 5, 10, 20 and 40% w/w concentrations, inoculated with L. squarrosulus and incubated at room temperature for 90 days. Levels of organic matter, pH, total hydrocarbon and elemental content (C, Cu, Fe, K, N, Ni, Zn and available P) were determined post-fungal treatment. Results indicate that contaminated soils inoculated with L. squarrosulus had increased organic ...
Source: Pakistan Journal of Biological Sciences: PJBS - June 15, 2008 Category: Biomedical Science Authors: Adenipekun CO, Isikhuemhen OS Tags: Pak J Biol Sci Source Type: journals

Microscopic evaluation of renal changes in experimental canine visceral leishmaniosis after chemo- and immunotherapy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was performed in Ahwaz, Khozestan province southwest to increase immune system and to reduce of the renal lesions. Treatment of dogs with visceral leishmaniosis is basically the same as the treatment of human. However, cure is not usually achieved, leaving the sacrifice of animal as the only feasible choice. The goal of this work was to test the therapeutic efficacy of N-methyl glutamic antimoate (glucnime), Mycobacterium vaccae adjuvant (SRL 172), alone and in association with L. major promastigote and the latter compound in association to glucantime, in dog with visceral leishmaniasis. In this trial 18, mixed ...
Source: Pakistan Journal of Biological Sciences: PJBS - June 15, 2008 Category: Biomedical Science Authors: Sayari M, Avizeh R, Barati F Tags: Pak J Biol Sci Source Type: journals

Effects of a locally produced blood meal on performance, carcass traits and nitrogen retention of broiler chickens.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The study was designed as a 2 x 5 factorial arrangement with main effects of feeding blood meal as a replacement of 0, 25, 50, 75 or 100% of dietary fish meal and the duration of this substitution at 1-42 or 21-42 days of age. One thousand 1 day old male Cobb 500 broiler chicks assigned randomly to each of 40 floor pens (25 birds/pen) and were fed five isonitrogenous and isocaloric feeds formulated to contain 20.38 and 17% crude protein and 2900 and 3000 kcal kg(-1) metabolizable for starter and grower periods, respectively. Duration of dietary manipulation didn't affect the measured parameters. More than 25% blood mea...
Source: Pakistan Journal of Biological Sciences: PJBS - June 15, 2008 Category: Biomedical Science Authors: Seifdavati J, Navidshad B, Seyedsharifi R, Sobhani A Tags: Pak J Biol Sci Source Type: journals

Incidence of human malaria infection in northern hilly region of Balochistan, adjoining with NWFP, Pakistan: district Zhob.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion it can be pointed out that the high incidence rate of P. vivax (51.8%:1681/3240) in Zhob district poses a significant health hazard because it may also lead to cerebral malaria as it was suggested by previous workers. PMID: 18819652 [PubMed - indexed for MEDLINE]
Source: Pakistan Journal of Biological Sciences: PJBS - June 15, 2008 Category: Biomedical Science Authors: Yasinzai MI, Kakarsulemankhel JK Tags: Pak J Biol Sci Source Type: journals

Variation in testicular histology of the spiny tailed lizard Uromastyx aegyptius microlepis during hibernation and active periods.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The testicular histology of the lizard, Uromastyx aegyptius microlepis were varied considerably during hibernation and activity periods, reaching maximum values in summer. The onset of winter induces testicular regression, reduced testis size, testis diameter, diameter and epithelial height of the seminiferous tubules and epididymal epithelial height. Animals exhibit testicular recrudescence during late winter (February and March) and maximum testicular volume occurred during June. The period of maximal testicular volume was positively correlated with increasing ambient temperature. The summer season induces the testic...
Source: Pakistan Journal of Biological Sciences: PJBS - June 15, 2008 Category: Biomedical Science Authors: Abu-Zinadah OA Tags: Pak J Biol Sci Source Type: journals

Screening of sugar beet tissue culture clones for resistance to rhizomania disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, sugar beet tissue culture clones were used to screen rhizomania resistant genotypes. At first, explants derived from shoot tips of sugar beet seedlings were transferred to shoot tip elongation media after surface sterilization. Then, the grown shoots were transferred to media containing various hormonal combinations NAA, BA, IBA and GA3 for multiplication, growth and rooting. Later, the clones were transferred to soil-peatmoss mixture were adapted to greenhouse conditions. For screening clones against rhizomania, the genotypes of adapted clones were selected and inoculated to rhizomania-infested soil. This e...
Source: Pakistan Journal of Biological Sciences: PJBS - June 15, 2008 Category: Biomedical Science Authors: Salari V, Norouzi P, Omidi M, Amiri R, Zandieh I Tags: Pak J Biol Sci Source Type: journals