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An introduction to liquid chromatography-mass spectrometry instrumentation applied in plant metabolomic analysesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Over the past decade the application of non-targeted high-throughput metabolomic analysis within the plant sciences has gained ever increasing interest and has truly established itself as a valuable tool for plant functional genomics and studies of plant biochemical composition. Whilst proton nuclear magnetic resonance (1H-NMR) spectroscopy is particularly appropriate for the analysis of bulk metabolites and gas chromatography mass spectrometry (GC-MS) to the analysis of volatile organic compounds (VOC's) and derivatised primary metabolites, liquid chromatography (LC)-MS is highly applicable to the analysis of a wide range...
Source: Phytochemical Analysis - November 20, 2009 Category: Biochemistry Authors: J. William Allwood, Royston Goodacre Source Type: journals

In vivo monitor oxidative burst induced by Cd2+ stress for the oilseed rape (Brassica napus L.) based on electrochemical microbiosensorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - This contribution presents the in vivo monitoring of the OB process induced by Cd2+ stress in oilseed rape by POPD-Pt-MP-Pt microbiosensor in real-time. The novel electrochemical microbiosensor not only facilitates the real-time study in plant self-defence response to the adverse environment such as Cd2+ stress, but also provides an effective tool for probing the self-defence mechanism in plants. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - November 11, 2009 Category: Biochemistry Authors: Qiao Xu, Fang Wei, Zhan Wang, Qin Yang, Yuan-Di Zhao, Hong Chen Source Type: journals

The analysis of pyrrolizidine alkaloids in Jacobaea vulgaris; a comparison of extraction and detection methodsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions - In terms of sensitivity and discrimination, formic acid extraction in combination with LC-MS/MS detection is the method of choice for analysing PAs (both free and N-oxides forms) in plant material. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - November 11, 2009 Category: Biochemistry Authors: Lotte Joosten, Patrick P. J. Mulder, Klaas Vrieling, Johannes A. van Veen, Peter G. L. Klinkhamer Source Type: journals

Tropane alkaloid profiling of hydroponic Datura innoxia mill. Plants inoculated with Agrobacterium rhizogenesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - In hydroponic conditions, microbes induced variations of the phytochemical levels. Addition of A. rhizogenes TR7 into the nutrient solutions improved the total hyoscyamine and scopolamine production. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - November 10, 2009 Category: Biochemistry Authors: Cyril Jousse, Thi Dao Vu, Thi Le Minh Tran, Mohamad Houssam Al Balkhi, Roland Molinié, Michèle Boitel-Conti, Serge Pilard, David Mathiron, Alain Hehn, Frédéric Bourgaud, Eric Gontier Source Type: journals

The photographer and the greenhouse: how to analyse plant metabolomics dataEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - This paper will familiarise non-specialised researchers with the main concepts in multivariate data analysis and allow them to develop and evaluate metabolomic data analyses more critically. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - November 10, 2009 Category: Biochemistry Authors: Jeroen J. Jansen, Suzanne Smit, Huub C.J. Hoefsloot, Age K. Smilde Source Type: journals

Application of NMR in plant metabolomics: techniques, problems and prospectsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present state-of-the-art of NMR in plant metabolomics is reviewed. Attention is paid to the different practical aspects of the application of NMR. The sample preparation, the measurement of the spectrum, quantitative aspects and data analysis are discussed. Each stage has its specific problems, which are pointed out and recommendations are made. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - November 10, 2009 Category: Biochemistry Authors: Jan Schripsema Source Type: journals

Two-dimensional J-resolved NMR spectroscopy: review of a key methodology in the metabolomics toolboxEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
One-dimensional (1D) 1H NMR spectroscopy remains a leading analytical technology in metabolomics. Advantages of this approach include relatively rapid spectral acquisition and NMR resonances that provide a direct measure of metabolite concentration based upon a single internal standard. Severe spectral congestion can, however, significantly hinder both metabolite identification and quantification. Two-dimensional 1H J-resolved (JRES) NMR spectroscopy retains many of the benefits of 1D NMR, but additionally disperses the overlapping resonances into a second dimension, reducing congestion and increasing metabolite specificit...
Source: Phytochemical Analysis - November 10, 2009 Category: Biochemistry Authors: Christian Ludwig, Mark R. Viant Source Type: journals

Sample preparation for plant metabolomicsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Sample preparation in plant metabolomics is a fundamental and critical step with important consequences for the accuracy of results. Depending on the analytical tools and the metabolites of interest, sample preparation has to be decided. However, the various methods reported in the literature have many steps in common and consequently the practical considerations concerning the pros and cons are similar. In this review, each step of the sample preparation - harvesting, drying, extraction and purification - will be discussed in detail. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - November 10, 2009 Category: Biochemistry Authors: Hye Kyong Kim, Rob Verpoorte Source Type: journals

Synthesis of a molecularly imprinted polymer for the solid-phase extraction of betulin and betulinic acid from plane barkEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - This study demonstrated the possibility of direct extraction of betulin and its structural analogues from plant extracts by MIP technology. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 28, 2009 Category: Biochemistry Authors: Berengere Claude, Cecile Viron-Lamy, Karsten Haupt, Philippe Morin Source Type: journals

Metabolic profiling of maize mutants deficient for two glutamine synthetase isoenzymes using 1H-NMR-based metabolomicsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - Changes in the metabolic profile of the GS mutants suggest that, when cytosolic GS activity is strongly reduced, either alternative metabolic pathways participate in the reassimilation of ammonium released during leaf protein remobilisation or that premature leaf senescence is induced when kernel set and kernel filling are affected. The accumulation of phenylalanine and tyrosine in the mutant plants indicates that lignin biosynthesis is altered, thus possibly affecting ear development. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 28, 2009 Category: Biochemistry Authors: Caroline Broyart, Jean-Xavier Fontaine, Roland Molinié, Dominique Cailleu, Thérèse Tercé-Laforgue, Frédéric Dubois, Bertrand Hirel, François Mesnard Source Type: journals

Metabolic response of tomato leaves upon different plant-pathogen interactionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions - NMR metabolomics is a potent platform to analyse the compounds involved in different plant infections. A broad response to different pathogenic infections was revealed at metabolomic levels in the plant. Also, metabolic specificity against each pathogen was observed. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 28, 2009 Category: Biochemistry Authors: M. Pilar López-Gresa, Federica Maltese, José María Bellés, Vicente Conejero, Hye Kyong Kim, Young Hae Choi, Robert Verpoorte Source Type: journals

Metabolomic analysis of host plant resistance to thrips in wild and cultivated tomatoesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The identification of acylsugars as a resistance factor for thrips in tomato proves that NMR-based metabolomics an important tool to study plant defences, providing fundamental information for the development and realisation of herbivore resistance breeding programmes in agricultural crops. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 28, 2009 Category: Biochemistry Authors: Mohammad Mirnezhad, Roman R. Romero-González, Kirsten A. Leiss, Young Hae Choi, Robert Verpoorte, Peter G. L. Klinkhamer Source Type: journals

Quantitative analysis of safranal in saffron extract and nanoparticle formulation by a validated high-performance thin-layer chromatographic methodEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The present method is useful for the quantitative and qualitative analysis of safranal and safranal-loaded nanoparticle formulation. It provides significant advantages in terms of greater specificity and rapid analysis. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 28, 2009 Category: Biochemistry Authors: Shadab Ahmad Pathan, Sanjar Alam, Gaurav K. Jain, Syed M. A. Zaidi, Sohail Akhter, Divya Vohora, Roop K. Khar, Farhan J. Ahmad Source Type: journals

A validated spectrophotometric method for quantification of prenylated flavanones in pacific propolis from TaiwanEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The proposed spectrophotometric procedure is useful for routine analyses of Macaranga-type propolis, because of its simplicity, repeatability and acceptable accuracy. Its application to a number of commercial samples could be used as a basis for standardisation and quality control of Pacific propolis. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 26, 2009 Category: Biochemistry Authors: Milena Popova, Chia-Nan Chen, Pen-Yuan Chen, Chung-Yang Huang, Vassya Bankova Source Type: journals

Comprehensive two-dimensional gas chromatography-mass spectrometry analysis of volatile constituents in Thai vetiver root oils obtained by using different extraction methodsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - In comparison with GC-MS, GC × GC-MS showed greater ability to differentiate the quality of essential oils obtained from diverse extraction conditions in terms of their volatile compositions and contents. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 20, 2009 Category: Biochemistry Authors: Patcharee Pripdeevech, Sugunya Wongpornchai, Philip J. Marriott Source Type: journals

Optimised separation procedures for the simultaneous assay of three plant hormones in liquid biofertilisersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - A simple and rapid sample preparation procedure followed by RP-HPLC with UV detection was optimised and developed for simultaneous quantification and identification of three plant hormones namely, IAA, GA3 and ABA in the liquid biofertilisers. This procedure allows quantification of the three plant hormones in their natural states without any prior derivatisation step. The results presented illustrate that the contents of the three plant hormones depended on the type of fruit wastes, fermentation time and the number of microorganisms found in liquid biofertilisers. This method can be extended to determine the ...
Source: Phytochemical Analysis - October 19, 2009 Category: Biochemistry Authors: Panadda Tansupo, Pirom Suwannasom, Devanand L. Luthria, Saksit Chanthai, Chalerm Ruangviriyachai Source Type: journals

Determination of polyphenolics in extracts of Potentilla species by high-performance thin-layer chromatography photodensitometry methodEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The proposed method was found to be relatively simple, specific, precise, sensitive and accurate and may be used for the routine assay of simultaneous determination of TRS, MBR and EA in other extracts and phytomedicines containing Potentilla species. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 19, 2009 Category: Biochemistry Authors: Micha[lstrok] Tomczyk, Agnieszka Bazylko, Anna Staszewska Source Type: journals

One-step separation and purification of rupestonic acid and chrysosptertin B from Artemisia rupestris L. by high-speed counter-current chromatographyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The rupestonic acid was separated in a single run by HSCCC. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 8, 2009 Category: Biochemistry Authors: Yi Yang, Dongya Gu, Abulimiti Yili, Yongxin Zhao, Dajun He, Haji Akber Aisa Source Type: journals

Simultaneous determination of flavonoids and phenylethanoids in the flowers of Verbascum densiflorum and V. phlomoides by high-performance liquid chromatographyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The HPLC method established is appropriate for the quality assurance and the differentiation of V. phlomoides and V. densiflorum samples. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 6, 2009 Category: Biochemistry Authors: Barbara Klimek, Monika Anna Olszewska, Magdalena Tokar Source Type: journals

Development and validation of a rapid capillary zone electrophoresis method for the determination of aconite alkaloids in aconite rootsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The developed CZE method was reliable for the quality control of aconites contained in herbal medicines. The method could also be used as an approach for toxicological studies. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 5, 2009 Category: Biochemistry Authors: Jing-Zheng Song, Quan-Bin Han, Chun-Feng Qiao, Paul Pui-Hay But, Hong-Xi Xu Source Type: journals

Analysis of sesquiterpene lactones in Eupatorium lindleyanum by HPLC-PDA-ESI-MS/MSEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - HPLC-PDA-ESI-MS/MS provides a new powerful approach to identify germacrane sesquiterpene lactones in E. lindleyanum rapidly and accurately. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - October 5, 2009 Category: Biochemistry Authors: Nian Yun Yang, Jin Ao Duan, Er Xin Shang, Li Juan Tian Source Type: journals

Differentiation of cultivation sources of Ganoderma lucidum by NMR-based metabolomics approachEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The easiness and transferability of our NMR-based approach should contribute to addressing an important aspect of quality control process of Ganoderma lucidum. We believe the method can be easily applied to other herbal medical products. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - September 24, 2009 Category: Biochemistry Authors: He Wen, Sunmi Kang, Youngmin Song, Yonghyun Song, Sang Hyun Sung, Sunghyouk Park Source Type: journals

Cytohistological and phytochemical study of madder root extracts obtained by ultrasonic and classical extractionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The study has shown the improvement of madder roots extraction both quantitatively and qualitatively using the efficiency of ultrasound-assisted extraction in comparison with magnetic agitation and reflux techniques. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - September 21, 2009 Category: Biochemistry Authors: Guillaume Cuoco, Carole Mathe, Paul Archier, Mohamed El Maâtaoui, Cathy Vieillescazes Source Type: journals

A TLC bioautographic method for the detection of [agr]- and [bgr]-glucosidase inhibitors in plant extractsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The screening test was able to detect inhibition of [agr]- and [bgr]-glucosidases by pure reference substances and by compounds present in complex matrices, such as plant extracts. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - September 21, 2009 Category: Biochemistry Authors: Claudia A. Simões-Pires, Bouchra Hmicha, Andrew Marston, Kurt Hostettmann Source Type: journals

Metabolic profiling of bioactive Pancratium canariense extracts by GC-MSEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The described method resulted in effective integration of both GC-EI-MS and LC-ESI-TOF-MS strategies, which permitted the identification of many metabolites, as well as the structural elucidation of new compounds with potential AChE inhibitory activity. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - September 21, 2009 Category: Biochemistry Authors: Laura Torras-Claveria, Strahil Berkov, Olga Jáuregui, Juli Caujapé, Francesc Viladomat, Carles Codina, Jaume Bastida Source Type: journals

Analysis of the monosaccharide composition of purified polysaccharides in Ganoderma atrum by capillary gas chromatographyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - This study demonstrated that the developed method could be applied as an accurate method for the compositional analysis of monosaccharides in the field of biological and biochemical study. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - September 8, 2009 Category: Biochemistry Authors: Yi Chen, Ming-Yong Xie, Yuan-Xing Wang, Shao-Ping Nie, Chang Li Source Type: journals

Mass spectrometry-based metabolomics oriented by correlation analysis for wound-induced molecule discovery: identification of a novel jasmonate glucosideEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The metabolomic approach developed has proved useful in the discovery of original jasmonates synthesised in response to plant wounding. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - September 8, 2009 Category: Biochemistry Authors: Gaetan Glauser, Julien Boccard, Serge Rudaz, Jean-Luc Wolfender Source Type: journals

Metabolomic analysis of Echinacea spp. by 1H nuclear magnetic resonance spectrometry and multivariate data analysis techniqueEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - A clear distinction between the three pharmaceutical species was possible and some useful metabolites were identified. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - September 8, 2009 Category: Biochemistry Authors: Michel Frédérich, Céline Jansen, Pascal de Tullio, Monique Tits, Vincent Demoulin, Luc Angenot Source Type: journals

Analysis of metabolic variation and galanthamine content in Narcissus bulbs by 1H NMREmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions - Since useful qualitative and quantitative information about the metabolic state of Narcissus bulbs can be obtained by 1H NMR, this method is useful for agricultural applications, and for quality control of raw material used in the pharmaceutical industry. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - September 8, 2009 Category: Biochemistry Authors: Andrea Lubbe, Barbora Pomaha[ccaron]ová, Young Hae Choi, Robert Verpoorte Source Type: journals

Application of microwave-assisted extraction coupled with high-speed counter-current chromatography for separation and purification of Dehydrocavidine from Corydalis saxicola BuntingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - Microwave-assisted extraction coupled with high-speed counter-current chromatography is a powerful tool for extraction, separation and purification of dehydrocavidine from C. saxicola. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - September 8, 2009 Category: Biochemistry Authors: Jianchao Deng, Xiaohua Xiao, Gongke Li, Guihua Ruan Source Type: journals

Optimisation of enzyme assisted extraction of silybin from the seeds of Silybum marianum by Box-Behnken experimental designEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The results suggest that EAE is a promising alternative for the extraction of silybin by the use of traditional ethanol extraction. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - August 11, 2009 Category: Biochemistry Authors: Hong Liu, Xueling Du, Qipeng Yuan, Lei Zhu Source Type: journals

Microwave-assisted extraction of total bioactive saponin fraction from Gymnema sylvestre with reference to gymnemagenin: a potential biomarkerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - Comparison with conventional extraction methods revealed that MAE could save considerable amounts of time and energy, whilst the reduction of volume of organic solvent consumed provides an ecofriendly feature. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - August 4, 2009 Category: Biochemistry Authors: Vivekananda Mandal, Saikat Dewanjee, Subhash C. Mandal Source Type: journals

O-glycoside sequence of pentacyclic triterpene saponins from Phytolacca bogotensis using HPLC-ESI/multi-stage tandem mass spectrometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - Electrospray ionisation in combination with tandem mass spectrometry has been established as a powerful tool for the profiling of saponins from roots of P. bogotensis. CID proved to be a useful tool for the characterisation and identification of known and novel saponins from the plant family Phytolaccaceae and can be used for quality control purposes of crude plant extracts. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - July 23, 2009 Category: Biochemistry Authors: Guillermo Montoya, Gabriel J. Arango, Matthias Unger, Ulrike Holzgrabe Source Type: journals

Evaluation of sample preparation methods for the analysis of papaya leaf proteins through two-dimensional gel electrophoresisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The described sample preparation method allows the proteomic analysis of papaya leaves by 2-DE and mass spectrometry (MALDI-TOF-MS/MS). The methods presented can be a starting point for the optimisation of sample preparation protocols for other plant species. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - July 22, 2009 Category: Biochemistry Authors: Silas Pessini Rodrigues, José Aires Ventura, R. B. Zingali, P. M. B. Fernandes Source Type: journals

Determination of lead content in medicinal plants by pre-concentration flow injection analysis-flame atomic absorption spectrometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The inclusion of a pre-concentration column in the flow manifold improved the sensitivity of the spectrometer. Thus, it was possible to determine the analyte at the ng/mL level in sample solutions of medicinal plants. This is a very important accomplishment, especially when the cumulative effect of heavy metals in living organisms is considered. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - July 16, 2009 Category: Biochemistry Authors: Marina M. A. Campos, Henry Tonuci, Silvana M. Silva, Bruna de S. Altoé, Dermeval de Carvalho, Eloisa A. M. Kronka, Ana M. S. Pereira, Bianca W. Bertoni, Suzelei de C. França, Carlos E. S. Miranda Source Type: journals

Characterisation of Phenolics in Flor-Essence® - a Compound Herbal product and its Contributing HerbsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - FE and its contributing herbs predominantly contain phenolics. This methodology can be applied to further develop full-scale validation of this product. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - July 15, 2009 Category: Biochemistry Authors: Ammar Saleem, Brendan Walshe-Roussel, Cory Harris, Muhammad Asim, Carmen Tamayo, Summer Sit, John Thor Arnason Source Type: journals

Pattern recognition and genetic algorithms for discrimination of orange juices and reduction of significant components from headspace solid-phase microextractionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions - SPME is a useful technique to investigate orange juice volatile composition and a flexible chemometric approach is able to correctly separate the juices. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - July 15, 2009 Category: Biochemistry Authors: Maurizio Rinaldi, Roberto Gindro, Massimo Barbeni, Gianna Allegrone Source Type: journals

A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in Cannabis sativa L.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - Results have shown that the HPTLC method is reproducible and accurate for the quantification of [Delta]9-THC in cannabis. The method is also useful for the qualitative screening of the main neutral cannabinoids found in cannabis cultivars. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - July 15, 2009 Category: Biochemistry Authors: Justin T. Fischedick, Ronald Glas, Arno Hazekamp, Rob Verpoorte Source Type: journals

Identification of a naturally-occurring 8-[[alpha]-D-glucopyranosyl-(1[rarr]6)-[beta]-D-glucopyranosyl]daidzein from cultivated kudzu rootEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The results indicate that puerarin diglycoside is one of the major isoflavonoids in kudzu root and has a significant impact on the preparation of highly water-soluble glycosylated puerarin. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - July 15, 2009 Category: Biochemistry Authors: Van Dao Nguyen, Byoung-Cheol Min, Myung-Ok Kyung, Jong-Tae Park, Byong Hoon Lee, Chung-Hyo Choi, Nam-Seok Seo, Yong-Ro Kim, Dong Uk Ahn, Sung-Joon Lee, Cheon-Seok Park, Jung-Wan Kim, Kwan-Hwa Park Source Type: journals

Microwave-assisted water extraction of green tea polyphenolsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - MWE appears more efficient than CWE at both 80 and 100°C, particularly for the extraction of flavanols and hydroxycinnamic acids. Although MWE at 100°C typically affords higher yields in total phenols, MWE at 80°C appears more convenient for the extraction of the green tea-specific and chemically sensitive flavanols. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - July 15, 2009 Category: Biochemistry Authors: Ezzohra Nkhili, Valerie Tomao, Hakima El Hajji, Es-Seddik El Boustani, Farid Chemat, Olivier Dangles Source Type: journals

Validation of a quantitative assay of arbutin using gas chromatography in Origanum majorana and Arctostaphylos uva-ursi extractsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Introduction - Arbutin is a skin-whitening agent that occurs naturally in the bark and leaves of various plants. It is commonly quantified in plant extracts and skin-whitening products by HPLC.Objective - To develop an alternative gas chromatographic method for the separation and quantification of arbutin in Origanum majorana and Arctostaphylos uva-ursi extracts.Methodology - N,O-Bis(trimethylsilyl)acetamide and trimethylchlorosilane were used as silylation reagents, and the gas chromatographic separation of silylated extracts and standards was performed using a DB-5 narrow bore column. GC-MS was used for the compound iden...
Source: Phytochemical Analysis - July 15, 2009 Category: Biochemistry Authors: Aline Lamien-Meda, Brigitte Lukas, Corinna Schmiderer, Chlodwig Franz, Johannes Novak Source Type: journals

Jasminum polyanthum Franch. as a natural source of (-)-methyl jasmonate: an alternative to the use of the synthetic standardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion - The approach proposed may enable the properties and effect of pure (-)-MJ on plant responses to be studied. The use of a natural source to obtain (-)-MJ is presented as an alternative to the enantioselective synthesis and enantiomeric resolution from the standard racaemic mixture. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytochemical Analysis)
Source: Phytochemical Analysis - July 15, 2009 Category: Biochemistry Authors: Gracia Patricia Blanch, Gema Flores, Maria del Mar Caja, Maria Luisa Ruiz del Castillo Source Type: journals

Simple and reliable methods for the determination of sixteen marker components for quality control of Daochi pill by HPLC coupled with diode array detectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Traditional Chinese medicine plays a very important role in the healthcare system of China and thus the quality control of medicinal herb products is of paramount concern.To establish a simple and effective high-performance liquid chromatography (HPLC) method to evaluate the quality of Daochi pill.Two HPLC methods were developed for the determination of 16 marker components in Daochi pills. In method A, the analytes were separated on a Lichrosorb C18 column using a gradient elution of methanol and 1% aqueous phosphoric acid (pH 2.9, adjusted by triethylamine). In method B, the separation was achieved on an Agilent Eclipse ...
Source: Phytochemical Analysis - June 24, 2009 Category: Biochemistry Authors: Binan Lu, Yuetao Liu, Lianhong Yin, Xiaona Wang, Jinyong Peng Source Type: journals

Direct analysis of curcumin in turmeric by DART-MSEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The new ion source technique, direct analysis in real time (DART) atmospheric pressure ionisation, allows high resolution mass measurements of gas, liquid and solid samples. As DART-MS produces [M + H]+ molecular ions of most compounds, relatively simple and clear mass spectra are obtained even of multi-component samples. In order to take advantage of the capacity of DART-MS for the real time analysis of individual compounds in natural raw materials, a pilot study was performed using the well-known antioxidant botanical drug, turmeric.To establish the analysis methods of curcumin and its derivatives from various types of s...
Source: Phytochemical Analysis - June 15, 2009 Category: Biochemistry Authors: Hye Jin Kim, Young Pyo Jang Source Type: journals

Determination of nickel, calcium and magnesium in xylem sap by flame atomic absorption spectrometry using a microsampling techniqueEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Knowledge of xylem sap chemical composition is important to the understanding of translocation, detoxification and tolerance mechanisms. However, the small amount of sample available often hampers its characterisation. Hence, low volume consumption techniques are needed for xylem sap analysis.To develop a microsampling technique for the determination of elements in xylem sap from different plants by flame atomic absorption spectrometry (FAAS).The microsampling device was optimised in terms of sample volume and integration time. The analytical characteristics of the microsampling technique (µ-FAAS) were established and com...
Source: Phytochemical Analysis - June 15, 2009 Category: Biochemistry Authors: Sheila Alves, Maria L. Simões Gonçalves, Margarida M. Correia dos Santos Source Type: journals

A retention index calculator simplifies identification of plant volatile organic compoundsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Plant volatiles (PVOCs) are important targets for studies in natural products, chemotaxonomy and biochemical ecology. The complexity of PVOC profiles often limits research to studies targeting only easily identified compounds. With the availability of mass spectral libraries and recent growth of retention index (RI) libraries, PVOC identification can be achieved using only gas chromatography coupled to mass spectrometry (GCMS). However, RI library searching is not typically automated, and until recently, RI libraries were both limited in scope and costly to obtain.To automate RI calculation and lookup functions commonly ut...
Source: Phytochemical Analysis - June 15, 2009 Category: Biochemistry Authors: Mary Lucero, Rick Estell, María Tellez, Ed Fredrickson Source Type: journals

Qualitative and quantitative determination of seven triterpene acids in Eriobotrya japonica Lindl. by high-performance liquid chromatography with photodiode array detection and mass spectrometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The leaves of Eriobotrya japonica are used for the treatment of diabetes mellitus, chronic bronchitis, coughs and skin diseases. No method is currently available, however, by which to assess the quality of the crude herb on the basis of the quantitative profile of the main bioactive triterpene acids present. To develop a simple and accurate HPLC-UV (photodiode array detection) method for the simultaneous quantification of seven triterpene acids in the leaves of E. japonica.Separations were performed on an Ultimate XB-C18 column by gradient elution using methanol:formic acid. Analytes were identified by HPLC coupled with el...
Source: Phytochemical Analysis - May 28, 2009 Category: Biochemistry Authors: Er-Na Li, Jian-Guang Luo, Ling-Yi Kong Source Type: journals

NMR and UPLC-qTOF-MS/MS characterisation of novel phenylethanol derivatives of phenylpropanoid glucosides from the leaves of strawberry (Fragaria × ananassa cv. Jonsok)Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Strawberry (Fragaria × ananassa) is rich in polyphenols, particularly anthocyanins, flavonols, condensed tannins and ellagic tannins. In addition to the fruits, the leaves of strawberry also contain a wide range of phenolic compound classes, but have not been investigated to the same extent as the fruit.To characterise a metabolite group present in the leaves of strawberry, that was not amenable for identification based on earlier information available in the literature.Methanolic extracts of strawberry leaves were analysed by UPLC-qTOF-MS/MS and iterative quantum mechanical NMR spectral analysis.The structures of phenyle...
Source: Phytochemical Analysis - May 10, 2009 Category: Biochemistry Authors: Kati Hanhineva, Pasi Soininen, Mikko J. Anttonen, Harri Kokko, Ilana Rogachev, Asaph Aharoni, Reino Laatikainen, Sirpa Kärenlampi Source Type: journals

Discrimination of three medicinal materials from the Citrus genus by HPLC fingerprint coupled with two complementary softwareEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pericarpium Citri Reticulatae, Pericarpium Citri Reticulatae Viride and Fructus Aurantii come from the fruit of Citrus genus and possess analogous pharmacological activities. Either two or three of them are often present in one preparation. However, there is no standard method to differentiate the three herbal medicines for quality control.To develop a fingerprint method for authentication of these three herbal medicines by HPLC.Methanol extracts were analysed by HPLC, with a mobile phase of 0.1% phosphoric acid in water (A) and acetonitrile (B) in a gradient programme. The flow rate was set at 0.8 mL/min and UV detection ...
Source: Phytochemical Analysis - May 8, 2009 Category: Biochemistry Authors: Xue-Mei Qin, Yun-Tao Dai, Li-Zeng Zhang, Xiao-Qing Guo, Hong-Xia Shao Source Type: journals

Product-specific sample clean-up and HPLC analysis of aflatoxins for a dietary productEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A sample clean-up procedure has been developed for a complex dietary ingredient, Unigen Inc. (Lacey, WA, USA) product A (a proprietary mixture of Scutellaria baicalensis and Acacia catechu extracts), for the determination of aflatoxins by HPLC.To develop an appropriate sample specific clean-up procedure that removes interferences from the sample for the determination of total aflatoxins B1, B2, G1 and G2 at or lower than 20 ppb by HPLC.The sample specific clean-up procedure was developed based on the modification of AOAC method 990.33.Coextract interferences were removed with the clean-up procedure. The recovery of total a...
Source: Phytochemical Analysis - May 8, 2009 Category: Biochemistry Authors: Michael Zahn, Mijeong Lee Jeong, Desheng Wang, Thao Trinh, Brooke Fay, Wenwen Ma Source Type: journals