Plant and Cell PhysiologyThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

Get the very latest Swine Flu news via the MedWorm Swine Flu RSS news feed - updated hourly from thousands of authoritative health and news sources.

• Contents
• Discussions

Pages: 1  2  3  4  5  6  7  8  9  10  11  12  13  14  15  

Resolving the Role of Plant Glutamate Dehydrogenase. I. in vivo Real Time Nuclear Magnetic Resonance Spectroscopy ExperimentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Plant and Cell Physiology)
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Labboun, S., Terce-Laforgue, T., Roscher, A., Bedu, M., Restivo, F. M., Velanis, C. N., Skopelitis, D. S., Moschou, P. N., Roubelakis-Angelakis, K. A., Suzuki, A., Hirel, B. Tags: Erratum Source Type: journals

A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2'-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in PlantsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A highly sensitive quantitative method for assaying nicotianamine (NA) and 2'-deoxymugineic acid (DMA) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) was developed. The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride. The amounts of NA and DMA in 10 µl of xylem sap from rice cultivated under iron (Fe)-sufficient and Fe-deficient conditions were quantified without concentration. In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe. In Fe-deficient plants, the concentration of NA...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Kakei, Y., Yamaguchi, I., Kobayashi, T., Takahashi, M., Nakanishi, H., Yamakawa, T., Nishizawa, N. K. Tags: Short Communications Source Type: journals

The Poplar GT8E and GT8F Glycosyltransferases are Functional Orthologs of Arabidopsis PARVUS Involved in Glucuronoxylan BiosynthesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The poplar GT8E and GT8F glycosyltransferases have previously been shown to be associated with wood formation, but their roles in the biosynthesis of wood components are not known. Here, we show that PoGT8E and PoGT8F are expressed in vessels and fibers during wood formation and their encoded proteins are predominantly located in the endoplasmic reticulum. We demonstrate that expression of PoGT8E and PoGT8F in the Arabidopsis parvus mutant rescues the defects in the content and structure of glucuronoxylan conferred by the parvus mutation. These findings suggest that PoGT8E and PoGT8F are involved in glucuronoxylan biosynth...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Lee, C., Teng, Q., Huang, W., Zhong, R., Ye, Z.-H. Tags: Short Communications Source Type: journals

Arabidopsis NIP1;1 Transports Antimonite and Determines Antimonite SensitivityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we identified an antimonite [Sb(III)] transporter from Arabidopsis thaliana. We examined the Sb(III) tolerance of the disruption mutant plants of arsenite [As(III)] transporters, nodulin 26-like intrinsic proteins (NIPs), since Sb(III) is similar to As(III) in structure. One of the mutants, nip1;1, showed Sb(III) tolerance and accumulated less Sb. Furthermore, yeast expressing NIP1;1 accumulated twice as much Sb as control. These data indicate that NIP1;1 transports Sb(III) and determines the Sb(III) sensitivity of A. thaliana. (Source: Plant and Cell Physiology)
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Kamiya, T., Fujiwara, T. Tags: Short Communications Source Type: journals

Arabidopsis Replication Protein A 70a is Required for DNA Damage Response and Telomere Length HomeostasisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we analyzed the functions of AtRPA70a, which is most similar to human RPA70 among four paralogs in Arabidopsis thaliana. RNA blot analysis showed that AtRPA70a is expressed ubiquitously in plant organs containing differentiated and meristematic tissues, while its expression was up-regulated in response to DNA damage stress. Yeast two-hybrid and co-immunoprecipitation analyses showed that AtRPA70a interacted preferentially with Arabidopsis RPA32a, one of two paralogs. Inactivation of AtRPA70a by T-DNA insertion did not affect growth under normal conditions, but resulted in increased sensitivity to genotoxic a...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Takashi, Y., Kobayashi, Y., Tanaka, K., Tamura, K. Tags: Regular Papers Source Type: journals

MYB83 Is a Direct Target of SND1 and Acts Redundantly with MYB46 in the Regulation of Secondary Cell Wall Biosynthesis in ArabidopsisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It has been proposed that the transcriptional regulation of secondary wall biosynthesis in Arabidopsis is controlled by a transcriptional network mediated by SND1 and its close homologs. Uncovering all the transcription factors and deciphering their interrelationships in the network are essential for our understanding of the molecular mechanisms underlying the transcriptional regulation of biosynthesis of secondary walls, the major constituent of wood and fibers. Here, we present functional evidence that the MYB83 transcription factor is another molecular switch in the SND1-mediated transcriptional network regulating secon...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: McCarthy, R. L., Zhong, R., Ye, Z.-H. Tags: Regular Papers Source Type: journals

Ectopic Overexpression of The Transcription Factor OsGLK1 Induces Chloroplast Development in Non-Green Rice CellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
For systematic and genome-wide analyses of rice gene functions, we took advantage of the full-length cDNA overexpresser (FOX) gene-hunting system and generated >12 000 independent FOX-rice lines from >25 000 rice calli treated with the rice-FOX Agrobacterium library. We found two FOX-rice lines generating green calli on a callus-inducing medium containing 2,4-D, on which wild-type rice calli became ivory yellow. In both lines, OsGLK1 cDNA encoding a GARP transcription factor was ectopically overexpressed. Using rice expression-microarray and northern blot analyses, we found that a large number of nucleus-encoded gene...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Nakamura, H., Muramatsu, M., Hakata, M., Ueno, O., Nagamura, Y., Hirochika, H., Takano, M., Ichikawa, H. Tags: Regular Papers Source Type: journals

Arabidopsis OPT6 is an Oligopeptide Transporter with Exceptionally Broad Substrate SpecificityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oligopeptide transporters (OPTs) are found in fungi, bacteria and plants. The nine Arabidopsis thaliana OPT genes are expressed mainly in the vasculature and are thought to transport tetra- and pentapeptides, and peptide-like substrates such as glutathione. Expression of AtOPT6 in Xenopus laevis oocytes demonstrated that AtOPT6 transports many tetra- and pentapeptides. In addition, AtOPT6 transported reduced glutathione (GSH), a tripeptide, but not oxidized glutathione (GSSG). Recent data showed that Candida albicans OPTs can transport peptides up to eight amino acids in length. AtOPT6 transported mammalian signaling pepti...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Pike, S., Patel, A., Stacey, G., Gassmann, W. Tags: Regular Papers Source Type: journals

High Temperatures Cause Male Sterility in Rice Plants with Transcriptional Alterations During Pollen DevelopmentEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Plant male reproductive development is highly organized and sensitive to various environmental stressors, including high temperature. We have established an experimental procedure to evaluate high temperature injury in japonica rice plants. High temperature treatment (39°C/30°C) starting at the microspore stage repeatedly reduced spikelet fertility in our system. Morphological observations revealed that pollen viability in plants exposed to high temperatures was lower than that in control plants. Most pollen grains in high temperature-treated plants displayed a normal round shape and stained reddish purple with Ale...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Endo, M., Tsuchiya, T., Hamada, K., Kawamura, S., Yano, K., Ohshima, M., Higashitani, A., Watanabe, M., Kawagishi-Kobayashi, M. Tags: Regular Papers Source Type: journals

The TL29 Protein is Lumen Located, Associated with PSII and Not an Ascorbate PeroxidaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The TL29 protein is one of the more abundant proteins in the thylakoid lumen of plant chloroplasts. Based on its sequence homology to ascorbate peroxidases, but without any supporting biochemical evidence, TL29 was suggested to be involved in the plant defense system against reactive oxygen species and consequently renamed to APX4. Our in vivo and in vitro analyses failed to show any peroxidase activity associated with TL29; it bound neither heme nor ascorbate. Recombinant overexpressed TL29 had no ascorbate-dependent peroxidase activity, and various mutational analyses aiming to convert TL29 into an ascorbate peroxidase f...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Granlund, I., Storm, P., Schubert, M., Garcia-Cerdan, J. G., Funk, C., Schroder, W. P. Tags: Regular Papers Source Type: journals

Rice BRITTLE CULM 5 (BRITTLE NODE) is Involved in Secondary Cell Wall Formation in the Sclerenchyma Tissue of NodesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Several brittle culm (bc) mutants known in grasses are considered excellent materials to study the process of secondary cell wall formation. The brittle phenotype of the rice bc5 (brittle node) mutant appears exclusively in the developed nodes, which is distinct from other bc mutants (bc1, 2, 3, 4, 6 and 7) that show the brittle phenotype in culms and leaves. To address the defects of the rice bc5 mutant in node-specific cell wall formation, we analyzed tissue morphology and cell wall composition. The bc5 mutation was found to affect the cell wall deposition of node sclerenchyma tissues at 1 week after heading, the stage a...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Aohara, T., Kotake, T., Kaneko, Y., Takatsuji, H., Tsumuraya, Y., Kawasaki, S. Tags: Regular Papers Source Type: journals

Differential Downward Stream of Auxin Synthesized at the Tip Has a Key Role in Gravitropic Curvature via TIR1/AFBs-Mediated Auxin Signaling PathwaysEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Since the early days of Darwin, monocot coleoptiles have been used to investigate indole-3-acetic acid (IAA) production, polar transport and tropisms. Here, using maize coleoptiles, we first showed that polar transport of IAA synthesized at the tip region is regulated by ZmPIN(s). Then, the TIR/AFBs-mediated auxin signaling pathway corresponds to the asymmetric IAA flow after gravi-stimulus, which results in tropic curvature. When [13C1115N2]Trp was applied to coleoptile tips, substantial amounts of the stable isotope were incorporated into IAA at the tip region, and the labeled IAA was transported in a polar manner at app...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Nishimura, T., Nakano, H., Hayashi, K.-i., Niwa, C., Koshiba, T. Tags: Regular Papers Source Type: journals

RESOPS: A Database for Analyzing the Correspondence of RNA Editing Sites to Protein Three-Dimensional StructuresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transcripts from mitochondrial and chloroplast DNA of land plants often undergo cytidine to uridine conversion-type RNA editing events. RESOPS is a newly built database that specializes in displaying RNA editing sites of land plant organelles on protein three-dimensional (3D) structures to help elucidate the mechanisms of RNA editing for gene expression regulation. RESOPS contains the following information: unedited and edited cDNA sequences with notes for the target nucleotides of RNA editing, conceptual translation from the edited cDNA sequence in pseudo-UniProt format, a list of proteins under the influence of RNA editi...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Yura, K., Sulaiman, S., Hatta, Y., Shionyu, M., Go, M. Tags: Rapid Paper Source Type: journals

Recent Progress in Plant Reproduction Research: The Story of the Male Gametophyte through to Successful FertilizationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Sexual reproduction is an important biological event not only for evolution but also for breeding in plants. It is a well known fact that Charles Darwin (1809–1882) was interested in the reproduction system of plants as part of his concept of ‘species’ and ‘evolution.’ His keen observation and speculation is timeless even in the current post-genome era. In the Darwin anniversary year of 2009, I have summarized recent molecular genetic studies of plant reproduction, focusing especially on male gametophyte development, pollination and fertilization. We are just beginning to understand the molecu...
Source: Plant and Cell Physiology - November 11, 2009 Category: Cytology Authors: Suzuki, G. Tags: Mini Review Source Type: journals

Alterations of Lysine Modifications on the Histone H3 N-Tail under Drought Stress Conditions in Arabidopsis thalianaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Plant and Cell Physiology)
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Kim, J.-M., To, T. K., Ishida, J., Morosawa, T., Kawashima, M., Matsui, A., Toyoda, T., Kimura, H., Shinozaki, K., Seki, M. Tags: Corrigendum Source Type: journals

Differences in Expression of the RBCS Multigene Family and Rubisco Protein Content in Various Rice Plant Tissues at Different Growth StagesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Four out of five members of the RBCS multigene family (OsRBCS2–OsRBCS5) were highly expressed in leaf blades of rice (Oryza sativa L.) irrespective of plant growth stage, whereas accumulation of all RBCS mRNAs in leaf sheaths, roots and developing spikelets was quite low. A highly positive correlation was observed between total RBCS and RBCL mRNA levels and Rubisco content at their maxima, irrespective of tissue and growth stage. The results indicate that the total RBCS mRNA level may be a primary determinant for maximal Rubisco protein content and that Rubisco gene expression is well coordinated through the whole li...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Suzuki, Y., Nakabayashi, K., Yoshizawa, R., Mae, T., Makino, A. Tags: Short Communication Source Type: journals

Antisense Expression of Mitochondrial ATP Synthase Subunits OSCP (ATP5) and {gamma} (ATP3) Alters Leaf Morphology, Metabolism and Gene Expression in ArabidopsisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Determination of the role of mitochondrial (mt) ATP synthesis in plant metabolism is complicated by chloroplastic ATP synthesis. To differentiate ATP synthesis from these two organelles, we created transgenic Arabidopsis plants in which two different subunits of the mt ATP synthase, the oligomycin sensitivity-conferring protein (OSCP) (=) (ATP5) and the (ATP3) subunit, were expressed individually in antisense orientation under the control of a dexamethasone-inducible promoter. The phenotypic effects of antisense expression were identical for both atp5 and atp3. Seedling lethality resulted from induction during germination ...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Robison, M. M., Ling, X., Smid, M. P. L., Zarei, A., Wolyn, D. J. Tags: Regular Papers Source Type: journals

New Dynamics in an Old Friend: Dynamic Tubular Vacuoles Radiate Through the Cortical Cytoplasm of Red Onion Epidermal CellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The textbook image of the plant vacuole sitting passively in the centre of the cell is not always correct. We observed vacuole dynamics in the epidermal cells of red onion (Allium cepa) bulbs, using confocal microscopy to detect autofluorescence from the pigment anthocyanin. The central vacuole was penetrated by highly mobile transvacuolar strands of cytoplasm, which were also visible in concurrent transmitted light images. Tubular vacuoles also extended from the large central vacuole and radiated through the cortical cytoplasm. These tubules were thin, having a diameter of about 1.5 µm, and were connected to the cen...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Wiltshire, E. J., Collings, D. A. Tags: Regular Papers Source Type: journals

The Involvement of Dual Mechanisms of Photoinactivation of Photosystem II in Capsicum annuum L. PlantsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
For plants, light is an indispensable resource. However, it also causes a loss of photosynthetic activity associated with photoinactivation of photosystem II (PSII). In studies of the mechanism of this photoinactivation, there are two conflicting hypotheses at present. One is that excess energy received by leaves, being neither utilized by photosynthesis nor dissipated safely in non-photochemical quenching, causes the photoinactivation. The other involves a two-step mechanism in which excitation of Mn by photons is the primary cause. In the former hypothesis, photoinactivation of PSII should not occur in low light that pro...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Oguchi, R., Terashima, I., Chow, W. S. Tags: Regular Papers Source Type: journals

Peptidyl-Prolyl Isomerase Activity in Chloroplast Thylakoid Lumen is a Dispensable Function of Immunophilins in Arabidopsis thalianaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chloroplast thylakoid lumen of Arabidopsis thaliana contains 16 immunophilins, five cyclophilins and 11 FK506-binding proteins (FKBPs), which are considered protein folding catalysts, although only two of them, AtFKBP13 and AtCYP20-2, possess peptidyl–prolyl cis/trans isomerase (PPIase) activity. To address the question of the physiological significance of this activity, we obtained and characterized Arabidopsis mutants deficient in the most active PPIase, AtFKBP13, and a double mutant deficient in both AtFKBP13 and AtCYP20-2. Two-dimensional gel electrophoresis of isolated thylakoid lumen, as well as immunoblotting ...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Ingelsson, B., Shapiguzov, A., Kieselbach, T., Vener, A. V. Tags: Regular Papers Source Type: journals

Temporal Expression Patterns of Hormone Metabolism Genes during Imbibition of Arabidopsis thaliana Seeds: A Comparative Study on Dormant and Non-Dormant AccessionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Seed imbibition is a prerequisite for subsequent dormancy and germination control. Here, we investigated imbibition responses of Arabidopsis seeds by transcriptomic and hormone profile analyses using dormant [Cape Verde Islands (Cvi)] and non-dormant [Columbia (Col)] accessions. Once imbibed, seeds of both accessions swelled most up to 3 h, reflecting water uptake. Microarray analysis showed that in both accessions, seeds imbibed for 15 min, 30 min and 1 h were less active in gene expression than at 3 h. More than 2,000 genes were either up-regulated or down-regulated in seeds imbibed for 3 h. Some genes up-regulated ...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Preston, J., Tatematsu, K., Kanno, Y., Hobo, T., Kimura, M., Jikumaru, Y., Yano, R., Kamiya, Y., Nambara, E. Tags: Regular Papers Source Type: journals

Blue Light-Induced Phototropism of Inflorescence Stems and Petioles is Mediated by Phototropin Family Members phot1 and phot2Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Phototropin family photoreceptors, phot1 and phot2, in Arabidopsis thaliana control the blue light (BL)-mediated phototropic responses of the hypocotyl, chloroplast relocation movement and stomatal opening. Phototropic responses in dark-grown tissues have been well studied but those in de-etiolated green plants are not well understood. Here, we analyzed phototropic responses of inflorescence stems and petioles of wild-type and phototropin mutant plants of A. thaliana. Similar to the results obtained from dark-grown seedlings, inflorescence stems and petioles in wild-type and phot2 mutant plants showed phototropic bending t...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Kagawa, T., Kimura, M., Wada, M. Tags: Regular Papers Source Type: journals

Resolving the Role of Plant Glutamate Dehydrogenase. I. in vivo Real Time Nuclear Magnetic Resonance Spectroscopy ExperimentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In higher plants the glutamate dehydrogenase (GDH) enzyme catalyzes the reversible amination of 2-oxoglutarate to form glutamate, using ammonium as a substrate. For a better understanding of the physiological function of GDH either in ammonium assimilation or in the supply of 2-oxoglutarate, we used transgenic tobacco (Nicotiana tabacum L.) plants overexpressing the two genes encoding the enzyme. An in vivo real time 15N-nuclear magnetic resonance (NMR) spectroscopy approach allowed the demonstration that, when the two GDH genes were overexpressed individually or simultaneously, the transgenic plant leaves did not synthesi...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Labboun, S., Terce-Laforgue, T., Roscher, A., Bedu, M., Restivo, F. M., Velanis, C. N., Skopelitis, D. S., Moshou, P. N., Roubelakis-Angelakis, K. A., Suzuki, A., Hirel, B. Tags: Regular Papers Source Type: journals

Effects of Site-Directed Mutations in the Chloroplast-Encoded ycf4 Gene on PSI Complex Assembly in the Green Alga Chlamydomonas reinhardtiiEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The chloroplast-encoded Ycf4 plays an essential role in PSI complex assembly in the green alga Chlamydomonas reinhardtii. To gain insight into how Ycf4 functions, we generated several mutants in which residues R120, E179 and/or E181, which are conserved among oxygenic photosynthetic organisms, were changed to A or Q. Although the single mutants R120A and R120Q accumulated 80% less Ycf4 than the wild type, they assembled a functional PSI complex and grew photosynthetically like the wild type. Thus we inferred that under laboratory growth conditions, wild-type cells accumulate a superfluous amount of Ycf4. Single mutants E17...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Onishi, T., Takahashi, Y. Tags: Regular Papers Source Type: journals

Differential Positioning of C4 Mesophyll and Bundle Sheath Chloroplasts: Aggregative Movement of C4 Mesophyll Chloroplasts in Response to Environmental StressesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In C4 plants, mesophyll (M) chloroplasts are randomly distributed along the cell walls, while bundle sheath (BS) chloroplasts are typically located in either a centripetal or centrifugal position. We investigated whether these intracellular positions are affected by environmental stresses. When mature leaves of finger millet (Eleusine coracana) were exposed to extremely high intensity light, most M chloroplasts aggregatively re-distributed to the BS side, whereas the intracellular arrangement of BS chloroplasts was unaffected. Compared with the homologous light-avoidance movement of M chloroplasts in C3 plants, it requires...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Yamada, M., Kawasaki, M., Sugiyama, T., Miyake, H., Taniguchi, M. Tags: Regular Papers Source Type: journals

Isozyme-Specific Modes of Activation of CTP:Phosphorylcholine Cytidylyltransferase in Arabidopsis thaliana at Low TemperatureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Arabidopsis thaliana increases cellular phosphatidylcholine (PC) content during cold acclimation by up-regulating PC biosynthesis. The A. thaliana genes CCT1 and CCT2 encode CTP:phosphorylcholine cytidylyltransferases (CCTs; EC 2.7.7.15), which regulate PC biosynthesis via the CDP–choline pathway. We isolated the T-DNA-tagged knockout mutants cct1 and cct2 of A. thaliana (Wassilevskaja; WS). CCT activity in cct1 and cct2 plants accounted for 29 and 79% to the cellular CCT activity of WS plants, respectively. When plants were exposed to 2°C for 7 d, CCT activity increased in both cct1 and cct2 plants, and immunobl...
Source: Plant and Cell Physiology - October 8, 2009 Category: Cytology Authors: Inatsugi, R., Kawai, H., Yamaoka, Y., Yu, Y., Sekiguchi, A., Nakamura, M., Nishida, I. Tags: Regular Papers Source Type: journals

The Arabidopsis 26S Proteasome Subunit RPN1a is Required for Optimal Plant Growth and Stress ResponsesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The current literature offers contradictory results regarding the role of the proteasome subunit RPN1a in Arabidopsis development. Here we show that plants lacking RPN1a are viable and have increased cell sizes, decreased heat shock tolerance, increased oxidative stress tolerance and other phenotypes characteristic for 26S proteasome subunit mutants. These results strengthen our contention that most of the phenotypes of 26S proteasome mutants in Arabidopsis described to date reflect a general impairment in 26S proteasome function rather than a specific defect of a single subunit, and suggest that the role of the RPN1a subu...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Wang, S., Kurepa, J., Smalle, J. A. Tags: Short Communication Source Type: journals

Transcriptome Analyses Revealed Diverse Expression Changes in ago1 and hyl1 Arabidopsis MutantsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
MicroRNAs (miRNAs) are 20–24 nucleotide endogenous regulatory molecules conserved in higher eukaryotes. In Arabidopsis, miRNAs are produced through step-wise cleavages of primary miRNA precursors (pri-miRNAs) by DICER-LIKE1 (DCL1). This cleavage step is also supported by a double-stranded RNA-binding protein, HYPONASTIC LEAVES1 (HYL1). In many cases, mature miRNA is predominantly incorporated into an endonuclease, ARGONAUTE1 (AGO1), which degrades miRNA-targeted mRNAs. Here, we examined and revealed whole genome transcriptomes in ago1-25 and hyl1-2 mutants using tiling arrays. The data in this paper are valuable for ...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Kurihara, Y., Kaminuma, E., Matsui, A., Kawashima, M., Tanaka, M., Morosawa, T., Ishida, J., Mochizuki, Y., Shinozaki, K., Toyoda, T., Seki, M. Tags: Short Communication Source Type: journals

Identification of cis-Localization Elements that Target Glutelin RNAs to a Specific Subdomain of the Cortical Endoplasmic Reticulum in Rice Endosperm CellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rice glutelin RNAs are localized to the cisternal endoplasmic reticulum (ER) by a regulated RNA transport process requiring specific cis-localization elements. We set out to identify these glutelin sequences by their dominant character of being able to re-direct the normal protein body ER localization of a maize 10 kDa -zein RNA to the cisternal ER. In situ RNA localization analysis showed that the glutelin RNA contains multiple cis-localization elements; two located at the 5' and 3' ends of the coding sequences and a third located within the 3'-untranslated region. These three regions contain two conserved sequences, sugg...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Washida, H., Kaneko, S., Crofts, N., Sugino, A., Wang, C., Okita, T. W Tags: Short Communication Source Type: journals

Overexpression of the Lily p70s6k Gene in Arabidopsis Affects Elongation of Flower Organs and Indicates TOR-Dependent Regulation of AP3, PI and SUP TranslationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The p70 ribosomal S6 kinase (p70s6k) signaling pathway plays a key role in regulating the cell cycle via translational regulation of specific 5'TOP mRNAs. However, the function of this signaling pathway is still poorly understood in plants. Ectopic expression of the lily putative p70s6k gene, LS6K1, resulted in up-regulation of NAP (NAC-LIKE, ACTIVATED BY AP3/PI) and PISTILLATA (PI) expression, and significantly inhibited cell expansion for petals and stamens, resulting in the male sterility phenotype in transgenic Arabidopsis. Sequence analysis revealed that the genes involved in petal and stamen development, such as APET...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Tzeng, T.-Y., Kong, L.-R., Chen, C.-H., Shaw, C.-C., Yang, C.-H. Tags: Regular Papers Source Type: journals

Dissection of the Relationship Between RACK1 and Heterotrimeric G-Proteins in ArabidopsisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mammalian receptor for activated C kinase 1 (RACK1) is a versatile scaffold protein, playing regulatory roles in multiple signal transduction pathways. Moreover, RACK1 interacts with the heterotrimeric G-proteins (G-proteins) and regulates some specific functions of Gβ. Although the protein sequences of both RACK1 and G-proteins are highly conserved in Arabidopsis, their relationship remains elusive. Here we provide genetic and biochemical evidence that Arabidopsis RACK1 and G-proteins may act through a mechanism that is distinct from their counterparts in mammals. Loss-of-function alleles of RACK1A (the most abundant...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Guo, J., Wang, S., Wang, J., Huang, W.-D., Liang, J., Chen, J.-G. Tags: Regular Papers Source Type: journals

Is the Photosystem II Complex a Monomer or a Dimer?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It is widely believed that the photosystem II (PSII) complex may function as a dimer in the thylakoid membrane. Here, we report experimental conversion from the monomeric PSII to the dimeric form by treatment with high concentrations of n-dodecyl-β-d-maltopyranoside (DM). The content of the PSII monomer in a PsbTc deletion mutant was much higher than in the wild type when solubilized with low concentrations of DM. However, upon treatment with higher concentrations of DM, the PSII dimer was also recovered in the PsbTc deletion mutant. These results suggest that there are at least two distinct processes of dimerization:...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Watanabe, M., Iwai, M., Narikawa, R., Ikeuchi, M. Tags: Regular Papers Source Type: journals

Oxygen Sensitivity of a Nitrogenase-like Protochlorophyllide Reductase from the Cyanobacterium Leptolyngbya boryanaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dark-operative protochlorophyllide (Pchlide) oxido-reductase (DPOR) is a nitrogenase-like enzyme that catalyzes Pchlide reduction, the penultimate step of chlorophyll a biosynthesis. DPOR is distributed widely among oxygenic phototrophs such as cyanobacteria, green algae and gymnosperms. To determine how DPOR operates in oxygenic photosynthetic cells, we constructed two shuttle vectors for overexpression of Strep-tagged L-protein (ChlL) and Strep-tagged NB-protein (ChlN–ChlB) in Leptolyngbya boryana (formerly Plectonema boryanum) and introduced them into mutants lacking chlL and chlB. Both transformants restored the ...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Yamamoto, H., Kurumiya, S., Ohashi, R., Fujita, Y. Tags: Regular Papers Source Type: journals

Enhancing Sucrose Synthase Activity in Transgenic Potato (Solanum tuberosum L.) Tubers Results in Increased Levels of Starch, ADPglucose and UDPglucose and Total YieldEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Sucrose synthase (SuSy) is a highly regulated cytosolic enzyme that catalyzes the conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate glucose and fructose. To determine the impact of SuSy activity in starch metabolism and yield in potato (Solanum tuberosum L.) tubers we measured sugar levels and enzyme activities in tubers of SuSy-overexpressing potato plants grown in greenhouse and open field conditions. We also transcriptionally characterized tubers of SuSy-overexpressing and -antisensed potato plants. SuSy-overexpressing tubers exhibited a substantial increase in starch, UDPg...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Baroja-Fernandez, E., Munoz, F. J., Montero, M., Etxeberria, E., Sesma, M. T., Ovecka, M., Bahaji, A., Ezquer, I., Li, J., Prat, S., Pozueta-Romero, J. Tags: Regular Papers Source Type: journals

Effects of Tobacco Ethylene Receptor Mutations on Receptor Kinase Activity, Plant Growth and Stress ResponsesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ethylene receptor is the first component of ethylene signaling that regulates plant growth, development and stress responses. Previously, we have demonstrated that tobacco subfamily 2 ethylene receptor NTHK1 had Ser/Thr kinase activity, and overexpression of NTHK1 caused large rosette, reduced ethylene sensitivity, and increased salt sensitivity in transgenic Arabidopsis plants. Here we found that N-box mutation in the NTHK1 kinase domain abolished the kinase activity and led to disruption of NTHK1 roles in conferring reduced ethylene sensitivity and salt sensitive response in transgenic Arabidopsis plants. However, N-box ...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Chen, T., Liu, J., Lei, G., Liu, Y.-F., Li, Z.-G., Tao, J.-J., Hao, Y.-J., Cao, Y.-R., Lin, Q., Zhang, W.-K., Ma, B., Chen, S.-Y., Zhang, J.-S. Tags: Regular Papers Source Type: journals

Involvement of C-22-Hydroxylated Brassinosteroids in Auxin-Induced Lamina Joint Bending in RiceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The rice lamina joint is ideal material for investigating the activity of brassinosteroids (BRs) and auxin because of its high sensitivity to these compounds. Using a series of rice BR biosynthetic and receptor mutants, we conducted lamina joint tests to elucidate the mechanism of cross-talk between BR and auxin signaling in lamina joint bending. In BR biosynthetic mutants d2 and brd1, which are defective in C-23 hydroxylase and C-6 oxidase, respectively, the lamina joint response to auxin was significantly higher than that of wild-type plants. The other BR-biosynthetic mutants, brd2, osdwarf4 and d11, which are defective ...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Nakamura, A., Fujioka, S., Takatsuto, S., Tsujimoto, M., Kitano, H., Yoshida, S., Asami, T., Nakano, T. Tags: Regular Papers Source Type: journals

Amyloplast Division Progresses Simultaneously at Multiple Sites in the Endosperm of RiceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The amyloplast, a form of differentiated plastid, proliferates in sink tissues, where it synthesizes and stores starch granules. Little is known about the molecular mechanism for amyloplast division and development. The rice (Oryza sativa) endosperm provides an excellent model system for studying molecular mechanisms involved in amyloplast division and starch synthesis. We compared amyloplast division processes in the endosperm of wild type and a mutant of ARC5, a member of the dynamin superfamily. Plant growth and fertility of arc5 were not significantly different from the wild type. Unlike binary fission of chloroplast i...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Yun, M.-S., Kawagoe, Y. Tags: Regular Papers Source Type: journals

Estimation of the Relative Sizes of Rate Constants for Chlorophyll De-excitation Processes Through Comparison of Inverse Fluorescence IntensitiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The paper derives a simple way to calculate the linear relationships between all separable groups of rate constants for de-excitation of Chl a excitation energy. This is done by comparison of the inverse values of chlorophyll fluorescence intensities and is based on the matrix model of Kitajima and Butler and on the lake model of energy exchange among PSII centers. Compared with the outputs of earlier, similar calculations, the results presented here add some linear comparisons of the relative sizes of rate constants without the need for F0' measurement. This enables us to regenerate the same alternative formula to calcula...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Kasajima, I., Takahara, K., Kawai-Yamada, M., Uchimiya, H. Tags: Regular Papers Source Type: journals

Auxin Amidohydrolases from Brassica rapa Cleave the Alanine Conjugate of Indolepropionic Acid as a Preferable Substrate: A Biochemical and Modeling ApproachEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Two auxin amidohydrolases, BrIAR3 and BrILL2, from Chinese cabbage [Brassica rapa L. ssp. pekinensis (Lour.) Hanelt] were produced by heterologous expression in Escherichia coli, purified, and screened for activity towards N-(indol-3-ylacetyl)–l-alanine (IAA-Ala) and the long-chain auxin–amino acid conjugates, N-[3-(indol-3-yl)propionyl]–l-alanine (IPA-Ala) and N-[4-(indol-3-yl)butyryl]–l-alanine (IBA-Ala). IPA-Ala was shown to be the favored substrate of both enzymes, but BrILL2 was approximately 15 times more active than BrIAR3. Both enzymes cleaved IBA-Ala and IAA-Ala to a lesser extent. The enzy...
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Savic, B., Tomic, S., Magnus, V., Gruden, K., Barle, K., Grenkovic, R., Ludwig-Muller, J., Salopek-Sondi, B. Tags: Regular Papers Source Type: journals

Arabidopsis Bile Acid:Sodium Symporter Family Protein 5 is Involved in Methionine-Derived Glucosinolate BiosynthesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we identified the bile acid:sodium symporter family protein 5 (BASS5) gene in Arabidopsis as a candidate transporter gene involved in methionine-derived GSL (Met-GSL) biosynthesis by means of transcriptome co-expression analysis. Knocking out BASS5 resulted in a decrease of Met-GSLs and concomitant increase of methionine. A transient assay using fluorescence fusion proteins indicated a chloroplastic localization of BASS5. These results supported the idea that BASS5 plays a role in translocation across the chloroplast membranes of the biosynthetic intermediates of Met-GSLs. (Source: Plant and Cell Physiology)
Source: Plant and Cell Physiology - September 7, 2009 Category: Cytology Authors: Sawada, Y., Toyooka, K., Kuwahara, A., Sakata, A., Nagano, M., Saito, K., Hirai, M. Y. Tags: Rapid Papers Source Type: journals

A Novel Carotenoid Derivative, Lutein 3-Acetate, Accumulates in Senescent Leaves of RiceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The biosynthetic pathway of lutein, the most abundant carotenoid in leaves, has been extensively studied, while its degradation pathway during senescence is poorly understood. We found that a novel carotenoid derivative, lutein 3-acetate, accumulates in senescent leaves of rice (Oryza sativa L.). The change in contents of lutein and lutein 3-acetate suggests that lutein is converted to lutein 3-acetate during senescence. Analysis of mutants involved in light-harvesting complex II (LHCII) accumulation and degradation suggests that the converting activity is induced during senescence and that only free lutein, not lutein bou...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Kusaba, M., Maoka, T., Morita, R., Takaichi, S. Tags: Short Communication Source Type: journals

Pollen-Specific SKP1-Like Proteins are Components of Functional SCF Complexes and Essential for Lily Pollen Tube ElongationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The ubiquitin–proteasome pathway mediates protein degradation and is involved in diverse aspects of plant development and differentiation, including pollen tube elongation and self-incompatibility. We characterized three lily (Lilium longiflorum) SKP1-like genes, LSK1–LSK3, that are specifically expressed in late pollen developmental stages and the elongating pollen tube. The encoded peptide sequences reveal that LSK1–LSK3 share high identity with Arabidopsis ASK1 and contain a putative N-terminal CUL1- and a C-terminal F-box-interacting domain. Yeast two-hybrid and in vitro affinity binding assays reveal...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Chang, L.-C., Guo, C.-L., Lin, Y.-S., Fu, H., Wang, C.-S., Jauh, G.-Y. Tags: Regular Papers Source Type: journals

Functional Analysis of FT and TFL1 Orthologs from Orchid (Oncidium Gower Ramsey) that Regulate the Vegetative to Reproductive TransitionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The FLOWERING LOCUS T (FT) and TERMINAL FLOWER 1 (TFL1) genes play crucial roles in regulating the vegetative to reproductive phase transition. Orthologs of FT/TFL1 (OnFT and OnTFL1) were isolated and characterized from Oncidium Gower Ramsey. OnFT mRNA was detected in axillary buds, leaves, pseudobulb and flowers. In flowers, OnFT was expressed more in young flower buds than in mature flowers and was predominantly expressed in sepals and petals. The expression of OnFT was regulated by photoperiod, with the highest expression from the 8th to 12th hour of the light period and the lowest expression at dawn. In contrast, the e...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Hou, C.-J., Yang, C.-H. Tags: Regular Papers Source Type: journals

Overexpression of BiP has Inhibitory Effects on the Accumulation of Seed Storage Proteins in Endosperm Cells of RiceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Seed storage proteins are specifically and highly synthesized during seed maturation and are deposited into protein bodies (PBs) via the endoplasmic reticulum (ER) lumen. The accumulation process is mediated by ER chaperones such as luminal binding protein (BiP) and protein disulfide isomerase (PDI). To examine the role of ER chaperones and the relationship between ER chaperones and levels of accumulation of seed storage proteins, we generated transgenic rice plants in which the rice BiP and PDI genes were overexpressed in an endosperm-specific manner under the control of the rice seed storage protein glutelin promoter. Th...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Yasuda, H., Hirose, S., Kawakatsu, T., Wakasa, Y., Takaiwa, F. Tags: Regular Papers Source Type: journals

Physiological Regulation of Coccolith Polysaccharide Production by Phosphate Availability in the Coccolithophorid Emiliania huxleyiEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study CP was experimentally characterized by HPLC analysis as an acid polysaccharide of mannose, galacturonic acid, xylose and rhamnose. Both calcification (estimated from 45Ca uptake) and CP production (estimated from uronic acid quantification) were stimulated under Pi-deficient conditions but strongly suppressed under Pi-sufficient conditions. When cells were transferred from Pi-sufficient to Pi-deficient conditions the production of neutral polysaccharides (NP)—storage glucans—ceased rapidly after a temporary increase in the presence of Pi, and CP production started to increase after Pi was almost d...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Kayano, K., Shiraiwa, Y. Tags: Regular Papers Source Type: journals

Light-Dependent and Light-Independent Protochlorophyllide Oxidoreductases in the Chromatically Adapting Cyanobacterium Fremyella diplosiphon UTEX 481Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The cyanobacterium Fremyella diplosiphon can alternate its light-harvesting pigments, a process called comple-mentary chromatic adaptation (CCA), allowing it to photosynthesize in green light (GL) and in fluctuating light conditions. Nevertheless, F. diplosiphon requires chlorophylls for photosynthesis under all light conditions. Two alternative enzymes catalyze the penultimate step of chlorophyll synthesis, light-dependent protochlorophyllide oxidoreductase (LPOR) and dark-operative protochlo-rophyllide oxidoreductase (DPOR). DPOR enzymatic activity is light independent, while LPOR requires light. Therefore, we hypothesiz...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Shui, J., Saunders, E., Needleman, R., Nappi, M., Cooper, J., Hall, L., Kehoe, D., Stowe-Evans, E. Tags: Regular Papers Source Type: journals

Dynamic Bridges--A Calponin-Domain Kinesin From Rice Links Actin Filaments and Microtubules in Both Cycling and Non-Cycling CellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Interaction and cross-talk between microtubules and actin microfilaments are important for the cell axis and polarity during plant cell growth and development, but little is known about the molecular components of this interaction. Plant kinesins with a calponin-homology domain (KCHs) were recently identified and associated with a putative role in microtubule–microfilament cross-linking. KCHs belong to a distinct branch of the minus end-directed kinesin subfamily and so far have only been identified in land plants including the mosses. Here we report the identification of a new KCH from rice (Oryza sativa), OsKCH1, a...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Frey, N., Klotz, J., Nick, P. Tags: Regular Papers Source Type: journals

Survey of Rice Proteins Interacting With OsFCA and OsFY Proteins Which Are Homologous to the Arabidopsis Flowering Time Proteins, FCA and FYEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The FCA protein is involved in controlling flowering time and plays more general roles in RNA-mediated chromatin silencing in Arabidopsis. It contains two RNA-binding domains and a WW domain. The FCA protein interacts with FY, a polyadenylation factor, via its WW domain. We previously characterized a rice gene, OsFCA, which was homologous to FCA. Here, we found that the OsFCA protein could interact through its WW domain with the following proteins: OsFY, a protein containing a CID domain present in RNA-processing factors such as Pcf11 and Nrd1; a protein similar to splicing factor SF1; a protein similar to FUSE splicing fa...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Jang, Y. H., Park, H.-Y., Kim, S.-K., Lee, J. H., Suh, M. C., Chung, Y. S., Paek, K.-H., Kim, J.-K. Tags: Regular Papers Source Type: journals

Regulation of HSD1 in Seeds of Arabidopsis thalianaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The hydroxysteroid dehydrogenase HSD1, identified in the proteome of oil bodies from mature Arabidopsis seeds, is encoded by At5g50600 and At5g50700, two gene copies anchored on a duplicated region of chromosome 5. Using a real-time quantitative reverse transcriptase–polymerase chain reaction (RT–PCR) approach, the accumulation of HSD1 mRNA was shown to be specifically and highly induced in oil-accumulating tissues of maturing seeds. HSD1 mRNA disappeared during germination. The activity of HSD1 promoter and the localization of HSD1 transcripts by in situ hybridization were consistent with this pattern. A compl...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Baud, S., Dichow, N. R., Kelemen, Z., d'Andrea, S., To, A., Berger, N., Canonge, M., Kronenberger, J., Viterbo, D., Dubreucq, B., Lepiniec, L., Chardot, T., Miquel, M. Tags: Regular Papers Source Type: journals

Differential Gene Expression Profiles of the Mitochondrial Respiratory Components in Illuminated Arabidopsis LeavesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Plant mitochondria have multiple energy-dissipating components in the respiratory chain. It is known that these components are induced under several stress conditions. Here we examined whether the gene expression pattern and its regulatory mechanism under high light (HL) conditions are different among the respiratory components in Arabidopsis leaves. Alternative oxidase (AOX) gene expression (AOX1a and AOX1c) and amount of protein were elevated after exposure to HL. In addition to AOX, the expression of other respiratory genes, including NDA1, NDB2, NDC1, UCP1, UCP5, COX6b and CI76, was also induced by HL. NDB2 was co-expr...
Source: Plant and Cell Physiology - August 9, 2009 Category: Cytology Authors: Yoshida, K., Noguchi, K. Tags: Regular Papers Source Type: journals