Proceedings of the National Academy of Sciences This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

A synthetic bacterial microcompartment as production platform for pyruvate from formate and acetate [Microbiology]
Bacteria do not have organelles. This textbook statement is as simple as it is false. An organelle is defined as a specialized structure inside a cell that has specific functions, such as the mitochondrium, the chloroplast, and the Golgi apparatus in a eukaryotic cell. Bacteria were believed to be devoid... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Volker Muller Tags: Microbiology, Commentaries Commentary Source Type: research

Metabolomic selection-based machine learning improves fruit taste prediction [Agricultural Sciences]
At least with regard to horticultural crops, we are currently experiencing a step change in crop breeding targets. Historically, breeders focused on high-yielding, resilient varieties; however, this has led to considerable dissatisfaction with modern varieties of fruits and vegetables (1). The recent increasing willingness of consumers to pay a premium... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Alisdair R. Fernie, Saleh Alseekh Tags: Agricultural Sciences, Commentaries Commentary Source Type: research

CSB-independent, XPC-dependent transcription-coupled repair in Drosophila [Biochemistry]
Drosophila melanogaster has been extensively used as a model system to study ionizing radiation and chemical-induced mutagenesis, double-strand break repair, and recombination. However, there are only limited studies on nucleotide excision repair in this important model organism. An early study reported that Drosophila lacks the transcription-coupled repair (TCR) form of... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Nazli Deger, Xuemei Cao, Christopher P. Selby, Saygin Gulec, Hiroaki Kawara, Evan B. Dewey, Li Wang, Yanyan Yang, Sierra Archibald, Berkay Selcuk, Ogun Adebali, Jeff Sekelsky, Aziz Sancar, Zhenxing Liu Tags: Biochemistry Biological Sciences Source Type: research

A PRC2-Kdm5b axis sustains tumorigenicity of acute myeloid leukemia [Cell Biology]
Acute myeloid leukemias (AMLs) with the NUP98-NSD1 or mixed lineage leukemia (MLL) rearrangement (MLL-r) share transcriptomic profiles associated with stemness-related gene signatures and display poor prognosis. The molecular underpinnings of AML aggressiveness and stemness remain far from clear. Studies with EZH2 enzymatic inhibitors show that polycomb repressive complex 2 (PRC2)... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Zhihong Ren, Arum Kim, Yu-Ting Huang, Wen-Chieh Pi, Weida Gong, Xufen Yu, Jun Qi, Jian Jin, Ling Cai, Robert G. Roeder, Wei-Yi Chen, Gang Greg Wang Tags: Cell Biology Biological Sciences Source Type: research

Structure-guided engineering of tick evasins for targeting chemokines in inflammatory diseases [Biochemistry]
As natural chemokine inhibitors, evasin proteins produced in tick saliva are potential therapeutic agents for numerous inflammatory diseases. Engineering evasins to block the desired chemokines and avoid off-target side effects requires structural understanding of their target selectivity. Structures of the class A evasin EVA-P974 bound to human CC chemokine ligands... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Ram Prasad Bhusal, Pramod Aryal, Shankar Raj Devkota, Rina Pokhrel, Menachem J. Gunzburg, Simon R. Foster, Herman D. Lim, Richard J. Payne, Matthew C. J. Wilce, Martin J. Stone Tags: Biochemistry Biological Sciences Source Type: research

Lack of association between pandemic chilblains and SARS-CoV-2 infection [Immunology and Inflammation]
An increased incidence of chilblains has been observed during the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic and attributed to viral infection. Direct evidence of this relationship has been limited, however, as most cases do not have molecular evidence of prior SARS-CoV-2 infection with PCR or antibodies. We enrolled... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Jeff R. Gehlhausen, Alicia J. Little, Christine J. Ko, Marc Emmenegger, Carolina Lucas, Patrick Wong, Jon Klein, Peiwen Lu, Tianyang Mao, Jillian Jaycox, Eric Wang, Yale IMPACT Team, Nelson Ugwu, Cate Muenker, Dilgash Mekael, Rhonda Q. Klein, Robert Patr Tags: Immunology and Inflammation, Coronavirus Related Biological Sciences Source Type: research

Reply to Nielsen et al.: Social mindfulness is associated with countries’ environmental performance and individual environmental concern [Social Sciences]
Nielsen et al. (1) argue that Van Doesum et al. (2) need to consider three points for their interpretation of a positive association between individual-level social mindfulness (SoMi) and environmental performance (EPI) at the country level (3). The association is weaker when 1) it is controlled for GDP and 2)... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Niels J. Van Doesum, Ryan O. Murphy, Marcello Gallucci, Efrat Aharonov–Maȷar, Ursula Athenstaedt, Wing Tung Au, Liying Bai, Robert Bohm, Inna Bovina, Nancy R. Buchan, Xiao–Ping Chen, Kitty B. Dumont, Jan B. Engelmann, Kimmo Eriksson, Hy Tags: Letters, Psychological and Cognitive Sciences Source Type: research

Role of ubiquitin-protein ligase UBR5 in the disassembly of mitotic checkpoint complexes [Biochemistry]
The mitotic (or spindle assembly) checkpoint system ensures accurate chromosome segregation in mitosis by preventing the onset of anaphase until correct bipolar attachment of sister chromosomes to the mitotic spindle is attained. It acts by promoting the assembly of a mitotic checkpoint complex (MCC), composed of mitotic checkpoint proteins BubR1,... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Sharon Kaisari, Shirly Miniowitz-Shemtov, Danielle Sitry-Shevah, Pnina Shomer, Guennadi Kozlov, Kalle Gehring, Avram Hershko Tags: Biochemistry Biological Sciences Source Type: research

Conservation of chromatin conformation in carnivores [Evolution]
High throughput chromatin conformation capture (Hi-C) of leukocyte DNA was used to investigate the evolutionary stability of chromatin conformation at the chromosomal level in 11 species from three carnivore families: Felidae, Canidae, and Ursidae. Chromosome-scale scaffolds (C-scaffolds) of each species were initially used for whole-genome alignment to a reference genome... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Marco Corbo, Joana Damas, Madeline G. Bursell, Harris A. Lewin Tags: Evolution Biological Sciences Source Type: research

ERK signaling dissolves ERF repression condensates in living embryos [Developmental Biology]
Phase separation underlies the organization of the nucleus, including the biogenesis of nucleoli and the packaging of heterochromatin. Here we explore the regulation of transcription factor condensates involved in gene repression by ERK signaling in gastrulating embryos of a simple proto-vertebrate (Ciona). ERK signaling induces nuclear export of the transcriptional... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Claire J. Weaver, Aleena L. Patel, Stanislav Y. Shvartsman, Michael S. Levine, Nicholas Treen Tags: Developmental Biology Biological Sciences Source Type: research

Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates [Biophysics and Computational Biology]
Periplasmic chaperones 17-kilodalton protein (Skp) and survival factor A (SurA) are essential players in outer membrane protein (OMP) biogenesis. They prevent unfolded OMPs from misfolding during their passage through the periplasmic space and aid in the disassembly of OMP aggregates under cellular stress conditions. However, functionally important links between interaction... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Neharika Chamachi, Andreas Hartmann, Mai Quynh Ma, Anna Svirina, Georg Krainer, Michael Schlierf Tags: Biophysics and Computational Biology Biological Sciences Source Type: research

Kurarinone alleviated Parkinson's disease via stabilization of epoxyeicosatrienoic acids in animal model [Immunology and Inflammation]
Parkinson’s disease (PD) is one of the most common neurodegenerative disorders and is characterized by loss of dopaminergic neurons in the substantia nigra (SN), causing bradykinesia and rest tremors. Although the molecular mechanism of PD is still not fully understood, neuroinflammation has a key role in the damage of dopaminergic... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Cheng-Peng Sun, Jun-Jun Zhou, Zhen-Long Yu, Xiao-Kui Huo, Juan Zhang, Christophe Morisseau, Bruce D. Hammock, Xiao-Chi Ma Tags: Immunology and Inflammation Biological Sciences Source Type: research

Casein kinase 1 and disordered clock proteins form functionally equivalent, phospho-based circadian modules in fungi and mammals [Biochemistry]
Circadian clocks are timing systems that rhythmically adjust physiology and metabolism to the 24-h day–night cycle. Eukaryotic circadian clocks are based on transcriptional–translational feedback loops (TTFLs). Yet TTFL-core components such as Frequency (FRQ) in Neurospora and Periods (PERs) in animals are not conserved, leaving unclear how a 24-h period is... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Daniela Marzoll, Fidel E. Serrano, Anton Shostak, Carolin Schunke, Axel C. R. Diernfellner, Michael Brunner Tags: Biochemistry Biological Sciences Source Type: research

Label-free sensing of cells with fluorescence lifetime imaging: The quest for metabolic heterogeneity [Medical Sciences]
Molecular, morphological, and physiological heterogeneity is the inherent property of cells which governs differences in their response to external influence. Tumor cell metabolic heterogeneity is of a special interest due to its clinical relevance to tumor progression and therapeutic outcomes. Rapid, sensitive, and noninvasive assessment of metabolic heterogeneity of cells... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Evgeny A. Shirshin, Marina V. Shirmanova, Alexey V. Gayer, Maria M. Lukina, Elena E. Nikonova, Boris P. Yakimov, Gleb S. Budylin, Varvara V. Dudenkova, Nadezhda I. Ignatova, Dmitry V. Komarov, Vladislav V. Yakovlev, Wolfgang Becker, Elena V. Zagaynova, Vl Tags: Medical Sciences Physical Sciences Source Type: research

Opinion: Nationally reported metrics can’t adequately guide transformative change in biodiversity policy [Ecology]
Biodiversity loss is rampant (1–3). To safeguard the future of life on Earth, there are growing calls for transformative change in biodiversity policy [i.e., for a fundamental and system-wide reorganization of how such policy is designed, implemented, and enforced across scales and sectors (1)]. Here we argue that to achieve... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - February 25, 2022 Category: Science Authors: Sara Fraixedas, Tomas Roslin, Laura H. Antao, Juha Poyry, Anna–Liisa Laine Tags: Opinions, Ecology, Front Matter Source Type: research