Rapid Communications in Mass Spectrometry : RCM
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. 
Subscribe to this data using MyMedWorm.
Subscribe to this data using GoogleReader.
Subscribe to this data using Bloglines.
Subscribe to this data using MyYahoo.
Get the very latest Swine Flu news via the MedWorm Swine Flu RSS news feed - updated hourly from thousands of authoritative health and news sources.
This page shows you the latest items in this publication.
1105 records returned
Metabolite identification in rat brain microdialysates by direct infusion nanoelectrospray ionization after desalting on a ZipTip and LTQ/Orbitrap mass spectrometry.
Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Analyzing brain microdialysate samples by mass spectrometry is challenging due to the high salt content of the artificial cerebral spinal fluid (aCSF), low analyte concentrations and small sample volumes collected. A drug and its major metabolites can be examined in brain microdialysates by targeted approaches such as selected reaction monitoring (SRM) which provides selectivity and high sensitivity. However, this approach is not well suited for metabolite profiling in the brain which aims to determine biotransformation pathways. Identifying minor metabolites, or metabolites that arise from brain metabolism, remains a ...
Source: Rapid Communications in Mass Spectrometry : RCM - November 16, 2009 Category: Chemistry Authors: Erve JC, Beyer CE, Manzino L, Talaat RE Tags: Rapid Commun Mass Spectrom Source Type: journals
The use of selected ion flow tube mass spectrometry to detect and quantify polyamines in headspace gas and oral air.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Polyamines are a class of aliphatic compounds which include putrescine, cadaverine, spermine and spermidine. They are involved in a variety of cellular processes and have been implicated in a number of different pathophysiological mechanisms. Polyamines are volatile compounds having a distinctive odour normally perceived as being unpleasant. The measurement of their abundance has, however, been restricted to compounds present in the aqueous phase. Using selected ion flow tube mass spectrometry (SIFT-MS) we have shown that the polyamines react with the ions H(3)O(+), NO(+) and O(2) (+) to form distinctive product ions a...
Source: Rapid Communications in Mass Spectrometry : RCM - November 16, 2009 Category: Chemistry Authors: Ross BM, Babay S, Ladouceur C Tags: Rapid Commun Mass Spectrom Source Type: journals
Determination of absolute photoionization cross-sections of aromatics and aromatic derivatives.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The absolute photoionization cross-sections of aromatics and aromatic derivatives including toluene, ethylbenzene, n-propylbenzene, o-xylene, m-xylene, p-xylene, 1,3,5-trimethylbenzene, styrene, phenylacetylene, indene, indane, 1-methylnaphthalene, benzyl alcohol and benzaldehyde were measured at the photon energy range from ionization thresholds to 11.7 eV. The experiments were performed by tunable synchrotron vacuum ultraviolet (VUV) photoionization mass spectrometry. Benzene was chosen as a calibration standard, since its photoionization cross-section is well known. Binary liquid mixtures of the investigated molecul...
Source: Rapid Communications in Mass Spectrometry : RCM - November 16, 2009 Category: Chemistry Authors: Zhou Z, Xie M, Wang Z, Qi F Tags: Rapid Commun Mass Spectrom Source Type: journals
Interaction of bacteria and ion-exchange particles and its potential in separation for matrix-assisted laser desorption/ionization mass spectrometric identification of bacteria in water.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Identification of microbial contaminants in drinking water is a challenge to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) due to low levels of microorganisms in fresh water. To avoid the time-consuming culture step of obtaining enough microbial cells for subsequent MALDI-MS analysis, a combination of membrane filtration and nanoparticles- or microparticles-based magnetic separation is a fast and efficient approach. In this work, the interaction of bacteria and fluidMAG-PAA, a cation-exchange superparamagnetic nanomaterial, was investigated by MALDI-MS analysis and transmission electron micro...
Source: Rapid Communications in Mass Spectrometry : RCM - November 16, 2009 Category: Chemistry Authors: Guo Z, Liu Y, Li S, Yang Z Tags: Rapid Commun Mass Spectrom Source Type: journals
Atmospheric pressure chemical ionization and fragmentation of aminomonosaccharides in H(2)O and D(2)O.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Aminomonosaccharides (glucosamine, galactosamine, and mannosamine) in H(2)O and D(2)O were ionized by atmospheric pressure chemical ionization (APCI) and their fragmentation patterns were investigated to identify them. All the aminomonosaccharides showed the same fragment ions but their relative ion intensities were different. Major product ions generated in H(2)O were [M + H](+), [M + H - H(2)O](+), and [2M + H - 3H(2)O](+), while in D(2)O were [M(D6) + D](+), [M(D6) + D - D(2)O](+), and [2M(D6) + D - D(2)O - 2HDO](+). At a high fragmentor voltage above 120 V, the relative ion intensities of the major product ions sho...
Source: Rapid Communications in Mass Spectrometry : RCM - November 16, 2009 Category: Chemistry Authors: Choi SS, Kim JC Tags: Rapid Commun Mass Spectrom Source Type: journals
Rapid separation and identification of major constituents in Pseudolarix kaempferi by ultra-performance liquid chromatography coupled with electrospray and quadrupole time-of-flight tandem mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
A rapid and reliable method based on ultra-performance liquid chromatography (UPLC) coupled with photodiode-array detection (PDA) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) has been developed for separation and identification of major constituents in extracts of root bark of Pseudolarix kaempferi Gordon (PKG). Identification of the constituents was carried out by interpretation of their retention times, UV absorption spectra, MS and MS/MS spectra, as well as the data provided by authentic standards and literatures. A total of 20 components were separated in only 8.0...
Source: Rapid Communications in Mass Spectrometry : RCM - November 16, 2009 Category: Chemistry Authors: Ye X, Tang M, Chen L, Peng A, Ma L, Ye H Tags: Rapid Commun Mass Spectrom Source Type: journals
Online deposition of nano-aerosol for matrix-assisted laser desorption/ionization mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
An online nano-aerosol sample deposition method for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is described in which matrix and analyte particles between 50 and 500 nm are aerodynamically focused onto a tight spot, ca. 200 microm in diameter, on the target plate under vacuum. MALDI analysis of the target is performed without additional sample preparation. The method is evaluated with insulin as the analyte and alpha-cyano-4-hydroxycinnamic acid (CHCA) as the matrix. Two preparation modes are compared with conventional dried-droplet deposition: mixture deposition where a single layer is deposi...
Source: Rapid Communications in Mass Spectrometry : RCM - November 16, 2009 Category: Chemistry Authors: Gao Y, Johnston MV Tags: Rapid Commun Mass Spectrom Source Type: journals
Structural investigation and elucidation of new communesins from a marine-derived Penicillium expansum Link by liquid chromatography/electrospray ionization mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study, we investigated a marine P. expansum strain exhibiting neuroactivity on a Diptera larvae bioassay. Bio-guided purification led to the isolation and the identification of communesin B as the main active compound by HRMS and (1)H and (13)C NMR. Liquid chromatography analyses with detection by electrospray ionization coupled with tandem mass spectrometry (LC/ESI-MS/MS) and high-resolution tandem mass spectrometry (LC/HRMS/MS) allowed the identification and characterization of four other known communesins (A, D, E and F) in the crude extract. A fragmentation model for dimethyl epoxide communesins was proposed af...
Source: Rapid Communications in Mass Spectrometry : RCM - November 16, 2009 Category: Chemistry Authors: Kerzaon I, Pouchus YF, Monteau F, Le Bizec B, Nourrisson MR, Biard JF, Grovel O Tags: Rapid Commun Mass Spectrom Source Type: journals
Identification of metabolites of adonifoline, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem and high-resolution mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study in vivo metabolism of adonifoline was studied for the first time by identifying the metabolites of adonifoline present in bile, urine and feces of rats using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS(n)) (ion trap) as well as liquid chromatography/electrospray ionization high-resolution mass spectrometry (LC/ESI-HRMS) (quadrupole-time of flight). In total 19 metabolites were identified and, among them, retronecine-N-oxides were confirmed by matching their fragmentation patterns with their fully characterized synthetic compounds. These metabolites are all involved in bot...
Source: Rapid Communications in Mass Spectrometry : RCM - November 16, 2009 Category: Chemistry Authors: Xiong A, Yang L, He Y, Zhang F, Wang J, Han H, Wang C, Bligh SW, Wang Z Tags: Rapid Commun Mass Spectrom Source Type: journals
Contribution of two approaches using electrospray ionization with multi-stage mass spectrometry for the characterization of linseed oil.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
A detailed characterization of triacylglycerols (TAGs) present in linseed oil samples from a local producer was performed using electrospray ionization and two mass spectrometric approaches; direct infusion multi-stage mass spectrometry (MS(n)) experiments and liquid chromatography/tandem mass spectrometry (LC/MS/MS) using non-aqueous reversed-phase chromatographic conditions. The combination of both approaches permitted the identification of 26 TAGs. Comparison of the two analytical approaches showed that discrimination of regioisomers was achieved from MS(3) data while other isobaric species were separated and identi...
Source: Rapid Communications in Mass Spectrometry : RCM - November 11, 2009 Category: Chemistry Authors: Loutelier-Bourhis C, Zovi O, Lecamp L, Bunel C, Lange CM Tags: Rapid Commun Mass Spectrom Source Type: journals
Removal of nitrite with sulfamic acid for nitrate N and O isotope analysis with the denitrifier method.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In environmental water samples that contain both nitrate (NO(3) (-)) and nitrite (NO(2) (-)), isotopic analysis of nitrate alone by all currently available methods requires pretreatment to remove nitrite. Sulfamic acid addition, used previously for this purpose (Wu JP, Calvert SE, Wong CS. Deep-Sea Research Part I - Oceanographic Research Papers 1997; 44: 287), is shown here to be compatible with the denitrifier method for both N and O isotope analysis of nitrate. Sulfamic acid at a pH of approximately 1.7 reduces nitrite to N(2). Samples are then neutralized with base prior to isotope analysis, to alleviate the buffer...
Source: Rapid Communications in Mass Spectrometry : RCM - November 11, 2009 Category: Chemistry Authors: Granger J, Sigman DM Tags: Rapid Commun Mass Spectrom Source Type: journals
Isotope dilution method for the assay of rotenone in olive oil and river waters by liquid chromatography/multiple reaction monitoring tandem mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
A new approach for the assay of rotenone (1) by isotope dilution mass spectrometry is presented. The extreme toxicity of rotenone, a natural phytodrug with insecticidal and piscicidal activity, recently banned by national and international environmental protection agencies, calls for the development of sensitive and accurate methods of analyses. Accordingly, the proposed protocol is based on the availability of the labeled internal standard rotenone-d(3) (3) which can be conveniently prepared by consecutive and specific mono-O-demethylation, and remethylation with methyl iodide-d(3). The sensitivity of the method is co...
Source: Rapid Communications in Mass Spectrometry : RCM - November 11, 2009 Category: Chemistry Authors: Mazzotti F, Di Donna L, Attya M, Gabriele B, Fazio A, Sindona G Tags: Rapid Commun Mass Spectrom Source Type: journals
Combined chemical and topographic imaging at atmospheric pressure via microprobe laser desorption/ionization mass spectrometry-atomic force microscopy.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The operational characteristics and imaging performance are described for a new instrument comprising an atomic force microscope coupled with a pulsed laser and a linear ion trap mass spectrometer. The operating mode of the atomic force microscope is used to produce topographic surface images having sub-micrometer spatial and height resolution. Spatially resolved mass spectra of ions, produced from the same surface via microprobe-mode laser desorption/ionization at atmospheric pressure, are also used to create a 100 x 100 microm chemical image. The effective spatial resolution of the image ( approximately 2 microm) was...
Source: Rapid Communications in Mass Spectrometry : RCM - November 11, 2009 Category: Chemistry Authors: Bradshaw JA, Ovchinnikova OS, Meyer KA, Goeringer DE Tags: Rapid Commun Mass Spectrom Source Type: journals
On-line solid-phase extraction coupled with liquid chromatography/electrospray ionization mass spectrometry for the determination of trace tributyltin and triphenyltin in water samples.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
On-line solid-phase extraction (SPE) for pre-concentration and sample cleanup is one strategy to reduce matrix effects and to simultaneously improve detection sensitivity in liquid chromatography/mass spectrometry (LC/MS). This paper describes an on-line SPE-LC/MS method for the determination of tributyltin (TBT) and triphenyltin (TPhT) at trace levels in water samples. The direct coupling of an on-line C(18) pre-column to LC/MS was used to pre-concentrate TBT and TPhT at trace levels from waters and to remove interfering matrix effects. Pre-concentration was followed by separation of TBT and TPhT on a C(18) column usi...
Source: Rapid Communications in Mass Spectrometry : RCM - November 10, 2009 Category: Chemistry Authors: Sun Q, Chen Z, Yuan D, Megharaj M, Naidu R Tags: Rapid Commun Mass Spectrom Source Type: journals
Gas chromatography coupled to electron capture negative ion mass spectrometry with nitrogen as the reagent gas - an alternative method for the determination of polybrominated compounds.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study, we explored the suitability of nitrogen as reagent gas for the determination of brominated flame retardants (polybrominated diphenyl ethers (PBDEs), polybrominated biphenyls (PBBs), allyl-2,4,6-tribromophenyl ether (ATE) and 2,3-dibromopropyl-2,4,6-tribromophenyl ether (DPTE)) and halogenated natural products (for instance, methoxylated tetrabrominated diphenylethers and polybrominated hexahydroxanthene derivatives). An ion source temperature of 250 degrees C and a nitrogen pressure of 7 Torr in the ion source gave the highest response for m/z 79 and 81 of virtually all investigated polybrominated compounds....
Source: Rapid Communications in Mass Spectrometry : RCM - November 10, 2009 Category: Chemistry Authors: Rosenfelder N, Vetter W Tags: Rapid Commun Mass Spectrom Source Type: journals
Validation of pentaacetylaldononitrile derivative for dual (2)H gas chromatography/mass spectrometry and (13)C gas chromatography/combustion/isotope ratio mass spectrometry analysis of glucose.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
A reference method to accurately define kinetics in response to the ingestion of glucose in terms of total, exogenous and endogenous glucose is to use stable-isotope-labelled compounds such as (2)H and (13)C glucose followed by gas chromatography/mass spectrometry (GC/MS) and gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) analysis. The use of the usual pentaacetyl (5Ac) derivative generates difficulties in obtaining accurate and reproducible results due to the two chromatographic peaks for the syn and anti isomers, and to the isotopic effect occurring during acetylation. Therefore, the pentaa...
Source: Rapid Communications in Mass Spectrometry : RCM - November 10, 2009 Category: Chemistry Authors: Sauvinet V, Gabert L, Qin D, Louche-Pélissier C, Laville M, Désage M Tags: Rapid Commun Mass Spectrom Source Type: journals
Analysis of a series of isomeric oligosaccharides by energy-resolved mass spectrometry: a challenge on homobranched trisaccharides.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Glycans exist as part of glycoproteins and glycolipids, which are involved in a variety of biological functions. The analysis of glycan structures, particularly that of structural isomers, is fundamentally important since isomeric glycans often show distinct functions; however, a method for their structural elucidation has not yet been established. Anomeric configurations, linkage positions and branching are the major factors in glycans and their alteration results in a large diversity of glycan structures. The analysis of vicinally substituted oligosaccharides is extremely difficult because the product ions formed in ...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Daikoku S, Widmalm G, Kanie O Tags: Rapid Commun Mass Spectrom Source Type: journals
Changes in the natural abundance of (13)CO(2)/(12)CO(2) in breath due to lipopolysacchride-induced acute phase response.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The natural abundance of carbon-13 in blood proteins increases during the cachectic state and may be a biomarker for disease status. We hypothesized a corresponding drop in the relative abundance of (13)C in breath CO(2). Using the lipopolysacchride (LPS)-induced endotoxemia model of the acute cachectic state, we demonstrated that the acute phase response causes shifts in the stable isotopes of carbon in exhaled CO(2) ((13)CO(2)/(12)CO(2) delta value) shortly after administration of LPS while glucocorticoid treatment does not. Mice were injected with LPS and stable isotopes of blood amino acids and carbon in exhaled CO...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Butz DE, Cook ME, Eghbalnia HR, Assadi-Porter F, Porter WP Tags: Rapid Commun Mass Spectrom Source Type: journals
Metabolomics profiling of extracellular metabolites in recombinant Chinese Hamster Ovary fed-batch culture.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
A metabolomics-based approach was used to time profile extracellular metabolites in duplicate fed-batch bioreactor cultures of recombinant Chinese Hamster Ovary (CHO) cells producing monoclonal IgG antibody. Culture medium was collected and analysed using a high-performance liquid chromatography (HPLC) system in tandem with an LTQ-Orbitrap mass spectrometer. An in-house software was developed to pre-process the LC/MS data in terms of filtering and peak detection. This was followed by principal component analysis (PCA) to assess variance amongst the samples, and hierarchical clustering to categorize mass peaks by their ...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Chong WP, Goh LT, Reddy SG, Yusufi FN, Lee DY, Wong NS, Heng CK, Yap MG, Ho YS Tags: Rapid Commun Mass Spectrom Source Type: journals
Analysis of [U-(13)C(6)]glucose in human plasma using liquid chromatography/isotope ratio mass spectrometry compared with two other mass spectrometry techniques.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The use of stable isotope labelled glucose provides insight into glucose metabolism. The (13)C-isotopic enrichment of glucose is usually measured by gas chromatography/mass spectrometry (GC/MS) or gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). However, in both techniques the samples must be derivatized prior to analysis, which makes sample preparation more labour-intensive and increases the uncertainty of the measured isotopic composition. A novel method for the determination of isotopic enrichment of glucose in human plasma using liquid chromatography/isotope ratio mass spectrometry (LC/IRM...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Schierbeek H, Moerdijk-Poortvliet TC, van den Akker CH, Te Braake FW, Boschker HT, van Goudoever JB Tags: Rapid Commun Mass Spectrom Source Type: journals
Mass spectrometry and UV-VIS spectrophotometry of ruthenium(II) [RuClCp(mPTA)(2)](OSO(2)CF(3))(2) complex in solution.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Ruthenium(II) complexes are of great interest as a new class of cancerostatics with advantages over classical platinum compounds including lower toxicity. The stability of the [RuClCp(mPTA)(2)](OSO(2)CF(3))(2) complex (I) (Cp cyclopentadienyl, mPTA N-methyl 1,3,5-triaza-7-phosphaadamantane) in aqueous solution was studied using spectrophotometry, matrix-assisted laser desorption/ionization (MALDI) and laser desorption/ionization (LDI) time-of-flight (TOF) mass spectrometry (MS). Spectrophotometry proves that at least three different reactions take place in water. Dissolution of I leads to fast coordination of water mol...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Peña-Méndez EM, González B, Lorenzo P, Romerosa A, Havel J Tags: Rapid Commun Mass Spectrom Source Type: journals
Online nanoelectrospray/high-field asymmetric waveform ion mobility spectrometry as a potential tool for discovery pharmaceutical bioanalysis.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Nanoelectrospray ionization (nESI) coupled online with high-field asymmetric waveform ion mobility spectrometry (FAIMS) for small molecule analysis in a discovery pharmaceutical setting was examined. A conventional capillary pump, autosampler and nESI source were used to introduce samples directly into the FAIMS device. The FAIMS device was used to separate gas-phase ions on a timescale that was compatible with the mass spectrometer. The capability of the nESI-FAIMS combination to efficiently remove metabolite interferences from the parent drug, and reduce ion suppression effects, was demonstrated. On average, 85% of t...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Hatsis P, Valaskovic G, Wu JT Tags: Rapid Commun Mass Spectrom Source Type: journals
Analysis of low-concentration gas samples with continuous-flow isotope ratio mass spectrometry: eliminating sources of contamination to achieve high precision.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study we determined (1) sources of contamination on a Gasbench II, (2) developed an analytical procedure to reduce contamination, and (3) identified an efficient, precise method for introducing sample gas into vials. We investigated three vial-filling procedures: (1) automated flush-fill (AFF), (2) vacuum back-fill (VBF), and (3) hand-fill (HF). Treatments were evaluated based on the time required for preparation, observed contamination, and multi-vial precision. The worst-case observed contamination was 4.5% of sample volume. Our empirical estimate showed that this level of contamination results in an error of 1.7...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Cambaliza MO, Harlow BA, Ubierna N, Mount GH, Marshall JD, Evans RD Tags: Rapid Commun Mass Spectrom Source Type: journals
Kinetics of albumin homocysteinylation measured with matrix-assisted laser/desorption ionization mass spectrometry versus with a radioactive tracer.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Homocysteinylation is a post-translational protein modification which involves homocysteine-thiolactone and may be responsible for many pathophysiological changes secondary to hyperhomocysteinemia. Therefore, methods to measure protein homocysteinylation in intact biological samples are required. We tested whether matrix assisted-laser/desorption ionization mass spectrometry (MALDI-MS) can detect time- and dose-dependent changes in in vitro homocysteine-thiolactone binding to human serum albumin. We have compared this method with a (35)S-thiolactone radioactive binding assay. Incubations with and without dithiothreitol...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Callegher E, Seraglia R, Vettore M, Puricelli L, Millioni R, Tessari P Tags: Rapid Commun Mass Spectrom Source Type: journals
Investigation of the identification point system adaptation in cocaine, benzoylecgonine and ecgonine methyl ester using a single quadrupole mass spectrometer.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
At present, no official criteria exist for drug identification using single quadrupole mass spectrometers although the European Union (EU) criteria for compound identification have been adopted. These criteria are evaluated with respect to the confirmation of cocaine and its metabolites by single quadrupole liquid chromatography/mass spectrometry (LC/MS) and problems are highlighted. Spiked samples, proficiency testing samples, certified reference materials and samples from real cases that had screened positive for cocaine derivatives by immunoassay were subjected to confirmation by LC/MS using single ion monitoring wi...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Pistos C, Karampela S, Papoutsis I, Athanaselis S, Spiliopoulou C, Maravelias C Tags: Rapid Commun Mass Spectrom Source Type: journals
Real-time trace detection and identification of chemical warfare agent simulants using recent advances in proton transfer reaction time-of-flight mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
This work demonstrates for the first time the potential of using recent developments in proton transfer reaction mass spectrometry for the rapid detection and identification of chemical warfare agents (CWAs) in real-time. A high-resolution (m/Deltam up to 8000) and high-sensitivity ( approximately 50 cps/ppbv) proton transfer reaction time-of-flight mass spectrometer (PTR-TOF 8000 from Ionicon Analytik GmBH) has been successfully used to detect a number of CWA simulants at room temperature; namely dimethyl methylphosphonate, diethyl methylphosphonate, diisopropyl methylphosphonate, dipropylene glycol monomethyl ether a...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Petersson F, Sulzer P, Mayhew CA, Watts P, Jordan A, Märk L, Märk TD Tags: Rapid Commun Mass Spectrom Source Type: journals
Reverse thin layer method for enhanced ion yield of oligosaccharides in matrix-assisted laser desorption/ionization.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
A sample preparation method that is suitable for sensitive detection of underivatized oligosaccharides by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been investigated. As compared with the conventional dried-droplet or ethanol (EtOH) recrystallization method, superior mass spectra in terms of ion yield and signal-to-noise (s/n) ratio were obtained when methanol (MeOH) was used as a solvent for the mixture of matrix and oligosaccharides. Based on these results, a new sample preparation method, named the 'reverse thin layer method', was developed. This method comprises two steps: first, ...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Nishikaze T, Amano J Tags: Rapid Commun Mass Spectrom Source Type: journals
Analysis of phenolic glycosides from Ilex litseaefolia using electrospray ionization quadrupole time-of-flight mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
PMID: 19902421 [PubMed - as supplied by publisher] (Source: Rapid Communications in Mass Spectrometry : RCM)
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Wu ZJ, Chen XZ, Fang DM, Qi HY, Ren WJ, Zhang GL Tags: Rapid Commun Mass Spectrom Source Type: journals
Selective photocatalytic degradation of poly(ethylene glycol) additives using TiO(2) surface-assisted laser desorption/ionization mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
PMID: 19902422 [PubMed - as supplied by publisher] (Source: Rapid Communications in Mass Spectrometry : RCM)
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Watanabe T, Okumura K, Nozaki K, Kawasaki H, Arakawa R Tags: Rapid Commun Mass Spectrom Source Type: journals
The successful inclusion of succinylacetone as a marker of tyrosinemia type I in Tuscany newborn screening program.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
PMID: 19902423 [PubMed - as supplied by publisher] (Source: Rapid Communications in Mass Spectrometry : RCM)
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: la Marca G, Malvagia S, Funghini S, Pasquini E, Moneti G, Guerrini R, Zammarchi E Tags: Rapid Commun Mass Spectrom Source Type: journals
A novel high-performance liquid chromatography/mass spectrometry method for improved selective and sensitive measurement of methotrexate polyglutamation status in human red blood cells.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The folate antagonist methotrexate is commonly used in low dose for treatment of rheumatoid arthritis and juvenile idiopathic arthritis. Therapeutic effects are attributed to intracellular levels of various methotrexate polyglutamates. The present methodology, combining a simple preparation step with ion-pairing reversed-phase liquid chromatography and electrospray ionization mass spectrometry, is suitable for the measurement of methotrexate and its polyglutamates(2-7), in human red blood cells. Sample preparation consists of perchloric acid protein precipitation followed by solid-phase extraction. Baseline separation ...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: van Haandel L, Becker ML, Leeder JS, Williams TD, Stobaugh JF Tags: Rapid Commun Mass Spectrom Source Type: journals
Detection of pathogenic Verticillium spp. using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
We describe for the first time a robust assay for reliable identification of Verticillium spp. using protein fingerprinting data obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS). Several sample preparation methods and matrices were investigated to improve mass spectra for the routine identification of six species of Verticillium spp.(Verticillium dahiliae, V. alboatrum, V. fungicola, V. nigrescens, and V. lecanii) by MALDI-TOF-MS. Using the optimized experimental method, we constructed a protein fingerprint database for six species of Verticillium and established a anal...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Tao J, Zhang G, Jiang Z, Cheng Y, Feng J, Chen Z Tags: Rapid Commun Mass Spectrom Source Type: journals
Comprehensive analysis of short peptides in sera from patients with IgA nephropathy.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
We analyzed serum short peptides comprehensively to know whether they were useful to characterize IgA nephropathy (IgAN). Serum samples from 26 patients with untreated IgAN and 25 healthy donors were tested. Short peptides with molecular weights of approximately 7 kDa, purified from the serum samples by magnetic-beads-based weak cation exchange, were detected by mass spectrometry. Then the peptide peaks detected were subjected to the multivariate data analysis by SIMCA-P+(R) containing principal component analysis (PCA) and orthogonal partial-least-squares-discriminate analysis (OPLS-DA). A total of 92 peptide peaks we...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Kaneshiro N, Xiang Y, Nagai K, Kurokawa MS, Okamoto K, Arito M, Masuko K, Yudoh K, Yasuda T, Suematsu N, Kimura K, Kato T Tags: Rapid Commun Mass Spectrom Source Type: journals
Matrix-free thin-layer chromatography/laser desorption ionization mass spectrometry for facile separation and identification of medicinal alkaloids.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
This study integrates for the first time conventional silica gel TLC and laser desorption ionization mass spectrometry (LDI-MS) thus eliminating the need for any external matrix. Successful separation of berberine (R(f) = 0.56) and palmatine (R(f) = 0.46) from Berberis barandana including their identification by MS are demonstrated. Furthermore, a robust electrospray ionization (ESI)-MS method utilizing residual sample from TLC for quantification of berberine applying selected reaction monitoring and standard addition method is presented. The amount of berberine in the plant root prepared for the study was determined to be...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Shariatgorji M, Spacil Z, Maddalo G, Cardenas LB, Ilag LL Tags: Rapid Commun Mass Spectrom Source Type: journals
Phosphopeptide enrichment with stable spatial coordination on a titanium dioxide coated glass slide.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Recent advances in phosphoproteomics have established powerful tools to analyze phosphorylation events. However, their spatial localization is lost due to sample homogenization procedures prior to the analysis. Imaging mass spectrometry (IMS) has emerged as a method to visualize the spatial distribution of molecules in tissue samples, but its application is still limited to relatively abundant molecules. Due to low phosphorylation stoichiometry, direct detection and imaging of protein phosphorylation by MS has not been achieved yet. Therefore we have developed a novel phosphopeptide enrichment strategy as a potential t...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Imanishi SY, Kouvonen P, Smått JH, Heikkilä M, Peuhu E, Mikhailov A, Ritala M, Lindén M, Corthals GL, Eriksson JE Tags: Rapid Commun Mass Spectrom Source Type: journals
Electrospray ionization mass spectrometry studies of cyclodextrin-carboxylate ion inclusion complexes.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Aqueous solutions containing simple model aliphatic and alicyclic carboxylic acids (surrogates 1-4) were studied using negative ion electrospray mass spectrometry (ESI-MS) in the presence and absence of alpha-, beta-, and gamma-cyclodextrin. Molecular ions were detected corresponding to the parent carboxylic acids and complexed forms of the carboxylic acids; the latter corresponding to non-covalent inclusion complexes formed between carboxylic acid and cyclodextrin compounds (e.g., beta-CD, alpha-CD, and gamma-CD). The formation of 1:1 non-covalent inclusion cyclodextrin-carboxylic complexes and non-inclusion forms of ...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Mohamed MH, Wilson LD, Headley JV, Peru KM Tags: Rapid Commun Mass Spectrom Source Type: journals
Photocrosslinking of a novel alpha,omega-unsaturated copolyamide: mass spectrometric study on model compounds with benzophenone as photoinitiator.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The aim of this work was to understand the reactions involved in the photocrosslinking processes of a alpha,omega-unsaturated copolyamide foreseen as a new UV-curable powder coating. The crosslinking reaction was photoinitiated with benzophenone. In this paper, the photochemical reaction between benzophenone and several model compounds was investigated. The model compounds contained functional groups which could be present in copolyamide. The products resulting from UV curing were identified using a combination of high-resolution mass spectrometry and MS(n) experiments. The characterization of the products allowed loca...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Mintsa MN, Lecamp L, Loutelier-Bourhis C, Lange CM, Baumgarten S, Bunel C Tags: Rapid Commun Mass Spectrom Source Type: journals
Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip(R)) for peptide purification, rather than liquid chromatography/mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of a...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Buckley M, Collins M, Thomas-Oates J, Wilson JC Tags: Rapid Commun Mass Spectrom Source Type: journals
Isotope abundance analysis for improved sample identification with tandem mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Tandem mass spectrometry (MS/MS) is widely used for trace level sample analysis in complex mixtures. However, sample identification in MS/MS is challenging and not as trustworthy as with electron ionization (EI) mass spectral libraries. This paper presents a novel method for the combination of isotope abundance analysis (IAA) and EI-MS/MS for improved sample identification even at trace level in complex matrices. Accordingly, the first quadrupole is scanned in a narrow range around the molecular ion group of isotopomers such as M(+), [M+1](+) and [M+2](+), Q2 serves for collision-induced dissociation to produce product...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Alon T, Amirav A Tags: Rapid Commun Mass Spectrom Source Type: journals
Investigation of the photochemical behaviour of pyrethroids lacking the cyclopropane ring by photo-solid-phase microextraction and gas chromatography/mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The characterization of by-products arising from the UV photodegradation of two insecticide pyrethroids lacking the cyclopropane ring (flucythrinate and fenvalerate) has been investigated by gas chromatography coupled with mass spectrometry (GC/MS). Twenty photoproducts were tentatively identified mainly based on the interpretation of the experimental mass spectra or by using reference mass spectra. Some of these compounds had not previously been detected. Furthermore, the generation of some of the photoproducts might be a matter for concern due to their potential toxicity. The corresponding photodegradation routes, in...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Fernandez-Alvarez M, Llompart M, Garcia-Jares C, Dagnac T, Lores M Tags: Rapid Commun Mass Spectrom Source Type: journals
Control of origin of larch wood: discrimination between European (Austrian) and Siberian origin by stable isotope analysis.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Most of the coniferous wood of non-European origin that is imported into the European Union (EU) has to be inspected phytosanitarily, to prevent the introduction and spread of harmful pests in EU territory. Wood importers have been caught declaring Siberian larch wood as being of European origin to save the costs of the inspection. Therefore, an unequivocal method has to be developed in addition to the traditional analysis of the tree ring density to identify incorrect declarations of larch wood origin. For this purpose, delta(18)O and delta(13)C analyses have been carried out to check if Siberian larch wood can be dis...
Source: Rapid Communications in Mass Spectrometry : RCM - November 9, 2009 Category: Chemistry Authors: Horacek M, Jakusch M, Krehan H Tags: Rapid Commun Mass Spectrom Source Type: journals
Simultaneous quantitation of testosterone, estradiol, ethinyl estradiol, and 11-ketotestosterone in fathead minnow fish plasma by liquid chromatography/positive atmospheric pressure photoionization tandem mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In the present work, for the first time, a rapid and sensitive liquid chromatography/positive atmospheric pressure photoionization tandem mass spectrometry (LC/APPI-MS/MS) method has been developed and validated for the simultaneous quantitation of testosterone, estradiol, ethinyl estradiol, and 11-ketotestosterone in fathead minnow fish plasma using no more than 10 microL of plasma. Compounds present in plasma were directly derivatized with dansyl chloride and 25 microL of the derivatized mixture was injected into the LC/APPI-MS/MS system. The gradient chromatographic elution was achieved on an Agilent Zorbax SB-C18 a...
Source: Rapid Communications in Mass Spectrometry : RCM - November 4, 2009 Category: Chemistry Authors: Zhang F, Bartels MJ, Geter DR, Carr MS, McClymount LE, Marino TA, Klecka GM Tags: Rapid Commun Mass Spectrom Source Type: journals
Differences in the stable isotope signatures of seabird egg membrane and albumen - implications for non-invasive studies.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study, we compared carbon and nitrogen stable isotopes of the membranes and albumen of individual eggs of three wild seabird species, the Southern Rockhopper penguin Eudyptes chrysocome, the Imperial shag Phalacrocorax atriceps albiventer, and the Thin-billed prion Pachyptila belcheri. We also included chicken eggs for comparison. Egg membranes were generally enriched in (13)C, compared with albumen. The difference varied between species, with 2.1 per thousand in Rockhopper penguins, 1.6 per thousand in Imperial shags, but only 0.5 per thousand in Thin-billed prions and 0.4 per thousand in chicken eggs. Egg membran...
Source: Rapid Communications in Mass Spectrometry : RCM - November 4, 2009 Category: Chemistry Authors: Quillfeldt P, McGill RA, Masello JF, Poisbleau M, van Noordwijk H, Demongin L, Furness RW Tags: Rapid Commun Mass Spectrom Source Type: journals
Rapid structural determination of alkaloids in a crude extract of Stemona saxorum by high-performance liquid chromatography/electrospray ionization coupled with tandem mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The electrospray ionization (ESI) mass spectrometric behavior of five Stemona alkaloids, stemokerrin, oxystemokerrin, oxystemokerrilactone, oxystemokerrin N-oxide and stemokerrin N-oxide, was studied using an ESI tandem mass technique (MS(n)). These compounds, isolated from Stemona saxorum endemic in Vietnam, represent a class of alkaloids containing a pyrido[1,2-a]azepine A,B-ring core with a 1-hydroxypropyl side chain attached to C-4. Their fragmentation pathways were elucidated by ESI-MS(n) results and the elemental composition of the major product ions was confirmed by accurate mass measurement. In order to rationa...
Source: Rapid Communications in Mass Spectrometry : RCM - November 4, 2009 Category: Chemistry Authors: Peng SY, Shi T, Wang YZ, Lin LG, Yang YM, Jiang HL, Ye Y Tags: Rapid Commun Mass Spectrom Source Type: journals
Ultraviolet photodissociation at 266 nm of phosphorylated peptide cations.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The objective of the experiments was to determine whether 266 nm UV irradiation on the phosphopeptide cations would induce unique peptide backbone dissociation. In addition, the general behavior of the phosphate loss (-80 or -98 Da) was monitored, particularly for those phosphopeptides with a phosphotyrosine residue that itself is a UV chromophore. For phosphopeptides with a UV chromophore, their photodissociation behavior was very similar to that of low-energy sustained off-resonance irradiation collisionally activated dissociation (SORI-CAD), with a few exceptions. For example, b- and y-type peptide backbone fragments we...
Source: Rapid Communications in Mass Spectrometry : RCM - November 4, 2009 Category: Chemistry Authors: Park S, Ahn WK, Lee S, Han SY, Rhee BK, Oh HB Tags: Rapid Commun Mass Spectrom Source Type: journals
Obituary: Dr William (Bill) R. Davidson: 1947-2009.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
PMID: 19844958 [PubMed - as supplied by publisher] (Source: Rapid Communications in Mass Spectrometry : RCM)
Source: Rapid Communications in Mass Spectrometry : RCM - October 19, 2009 Category: Chemistry Authors: Boyd B Tags: Rapid Commun Mass Spectrom Source Type: journals
Method development for direct detection of glycoproteins on aminophenylboronic acid functionalized self-assembled monolayers by matrix-assisted laser desorption/ionization mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
PMID: 19844961 [PubMed - as supplied by publisher] (Source: Rapid Communications in Mass Spectrometry : RCM)
Source: Rapid Communications in Mass Spectrometry : RCM - October 19, 2009 Category: Chemistry Authors: Jang KK, Kim YH, Lee KS, Choi IS, Kim S, Lee KB Tags: Rapid Commun Mass Spectrom Source Type: journals
Precursor ion scans for the targeted detection of stable-isotope-labeled peptides.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study we investigated the specific detection and identification of labeled proteins using acquisition methods based on Precursor Ion Scans (PIS) on a triple-quadrupole ion trap mass spectrometer. PIS-based methods were set to detect unique immonium ions originating from labeled peptides. Different labels and methods were tested in standard mixtures to optimize performance. We showed that, in comparison with an untargeted analysis on the same instrument, the approach allowed a several-fold increase in the specificity of detection of labeled proteins over unlabeled ones. The technique was applied to the identificatio...
Source: Rapid Communications in Mass Spectrometry : RCM - October 19, 2009 Category: Chemistry Authors: Colzani M, Bienvenut WV, Faes E, Quadroni M Tags: Rapid Commun Mass Spectrom Source Type: journals
Use of ion mobility mass spectrometry and a collision cross-section algorithm to study an organometallic ruthenium anticancer complex and its adducts with a DNA oligonucleotide.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
We report the development of an enhanced algorithm for the calculation of collision cross-sections in combination with Travelling-Wave ion mobility mass spectrometry technology and its optimisation and evaluation through the analysis of an organoruthenium anticancer complex [(eta(6)-biphenyl)Ru(II)(en)Cl](+). Excellent agreement was obtained between the experimentally determined and theoretically determined collision cross-sections of the complex and its major product ion formed via collision-induced dissociation. Collision cross-sections were also experimentally determined for adducts of this ruthenium complex with the si...
Source: Rapid Communications in Mass Spectrometry : RCM - October 19, 2009 Category: Chemistry Authors: Williams JP, Lough JA, Campuzano I, Richardson K, Sadler PJ Tags: Rapid Commun Mass Spectrom Source Type: journals
Rapid drug detection in oral samples by porous silicon assisted laser desorption/ionization mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study, the application of the technique is demonstrated with real samples from actual roadside testing. The analysis of oral samples resulted in detection of MA and MDMA with no extraction and analysis of THC after ethyl acetate extraction. We propose that, subject to miniaturization of a suitable mass spectrometer, this technique is well suited to underpin the deployment of oral fluid testing in the clinic, workplace and on the roadside. Copyright (c) 2009 John Wiley & Sons, Ltd.
PMID: 19844964 [PubMed - as supplied by publisher] (Source: Rapid Communications in Mass Spectrometry : RCM)
Source: Rapid Communications in Mass Spectrometry : RCM - October 19, 2009 Category: Chemistry Authors: Lowe RD, Guild GE, Harpas P, Kirkbride P, Hoffmann P, Voelcker NH, Kobus H Tags: Rapid Commun Mass Spectrom Source Type: journals
