Uremia Impedes Skeletal Myocyte Myomixer Expression and Fusogenic Activity: Implication for Uremic Sarcopenia
Am J Pathol. 2024 May;194(5):759-771. doi: 10.1016/j.ajpath.2024.01.005.ABSTRACTIn patients with chronic kidney disease (CKD), skeletal muscle mass and function are known to occasionally decline. However, the muscle regeneration and differentiation process in uremia has not been extensively studied. In mice with CKD induced by adenine-containing diet, the tibialis anterior muscle injured using a barium chloride injection method recovered poorly as compared to control mice. In the cultured murine skeletal myocytes, stimulation with indoxyl sulfate (IS), a representative uremic toxin, morphologically jeopardized the differen...
Source: The American Journal of Pathology - April 18, 2024 Category: Pathology Authors: Takaaki Higashihara Motoki Odawara Hiroshi Nishi Takehito Sugasawa Yumika Suzuki Satoshi Kametaka Reiko Inagi Masaomi Nangaku Source Type: research
Stromal rigidity stress accelerates pancreatic intraepithelial neoplasia progression and chromosomal instability via nuclear PTK2 localization
Am J Pathol. 2024 Apr 15:S0002-9440(24)00132-9. doi: 10.1016/j.ajpath.2024.02.023. Online ahead of print.ABSTRACTSince the mechanotransduction by stromal stiffness stimulates the rupture and repair of the nuclear envelope in pancreatic progenitor cells, accumulated genomic aberrations are under selection in the tumor microenvironment. Analysis of cell growth, micronuclei, and γH2AX foci links to mechanotransduction pressure in vivo during serial orthotopic passages of mouse KrasLSL-G12D/+;Trp53flox/flox;Pdx1-Cre (KPC) cancer cells in the tumor and in migrating through the size-restricted 3μm micropores. To search for pan...
Source: The American Journal of Pathology - April 17, 2024 Category: Pathology Authors: Li-Yun Ding Chia-Jung Chang Szu-Ying Chen Kuan-Lin Chen Yueh-Shan Li Yun-Chieh Wu Ting-Yi Hsu Hsin-Yu Ying Hsin-Yi Wu Michael W Hughes Chia-Yih Wang Chih-Han Chang Ming-Jer Tang Woei-Jer Chuang Yan-Shen Shan Chia-Jung Chang Po-Hsien Huang Source Type: research
Stromal rigidity stress accelerates pancreatic intraepithelial neoplasia progression and chromosomal instability via nuclear PTK2 localization
Am J Pathol. 2024 Apr 15:S0002-9440(24)00132-9. doi: 10.1016/j.ajpath.2024.02.023. Online ahead of print.ABSTRACTSince the mechanotransduction by stromal stiffness stimulates the rupture and repair of the nuclear envelope in pancreatic progenitor cells, accumulated genomic aberrations are under selection in the tumor microenvironment. Analysis of cell growth, micronuclei, and γH2AX foci links to mechanotransduction pressure in vivo during serial orthotopic passages of mouse KrasLSL-G12D/+;Trp53flox/flox;Pdx1-Cre (KPC) cancer cells in the tumor and in migrating through the size-restricted 3μm micropores. To search for pan...
Source: The American Journal of Pathology - April 17, 2024 Category: Pathology Authors: Li-Yun Ding Chia-Jung Chang Szu-Ying Chen Kuan-Lin Chen Yueh-Shan Li Yun-Chieh Wu Ting-Yi Hsu Hsin-Yu Ying Hsin-Yi Wu Michael W Hughes Chia-Yih Wang Chih-Han Chang Ming-Jer Tang Woei-Jer Chuang Yan-Shen Shan Chia-Jung Chang Po-Hsien Huang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of Type 17 Mucosal-Associated Invariant T Cells in Circulation Contributes to the Severity of Sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RAR-related orphan receptor γt in patients with sepsis. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA-sequencing analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in patients with sepsis. Cell-culture experiments demonstrated that phosphoinositide 3-kinase-LDHA signaling was required for RAR-related orphan receptor γt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 cells in sepsis. In su...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
This Month in AJP
Am J Pathol. 2024 Apr 8:S0002-9440(24)00126-3. doi: 10.1016/j.ajpath.2024.04.001. Online ahead of print.NO ABSTRACTPMID:38599460 | DOI:10.1016/j.ajpath.2024.04.001 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Source Type: research
Differentiation of type 17 MAIT cells in circulation contributes to the severity of sepsis
This study identified a higher proportion of circulating MAIT17 with expression of IL-17A and RORγt in sepsis patients. The proportion of MAIT17 was correlated with the severity of sepsis. Single-cell RNA sequencing (scRNA-seq) analysis revealed an enhanced expression of lactate dehydrogenase A (LDHA) in MAIT17 in sepsis patients. Cell-culture experiments demonstrated that Phosphoinositide 3-kinase (PI3K)-LDHA signaling was required for RORγt expression in MAIT17. Finally, the elevated levels of plasma IL-18 promoted the differentiation of circulating MAIT17 in sepsis. In summary, this study reveals a new role of circula...
Source: The American Journal of Pathology - April 10, 2024 Category: Pathology Authors: Xinying Li Sicheng Fu Hao Cheng Min Ma Zijian Song Jun Li Shuang Wu Chong Zhang Xiaoxia Wang Maoyu Tang Xuexue Pu Qiang Ji Jinquan Liang Zhibin Zhao Heinrich K örner Bin Li Min Shao Hua Wang Source Type: research
