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The efficiency of nuclear plasmid DNA delivery is a critical determinant of transgene expression at the single cell levelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The nuclear envelope that encloses the nucleus is a significant barrier to non-viral vectors and shrouds the relationship between the trafficking of plasmid DNA to the nucleus and expression of an encoded transgene. Here, we use a novel single cell approach to quantify nuclear import of plasmid DNA following non-viral transfection and correlate this with reporter gene expression.Through the fractionation of intact nuclei from HeLa cells, the intranuclear copy number of plasmid DNA was quantified after transfection with either polyethylenimine (PEI) or LipofectAMINE2000 (LFA). Importantly, the use of a reporter protein that...
Source: The Journal of Gene Medicine - November 4, 2009 Category: Genetics & Stem Cells Authors: Dominic J. Glover, Denisse L. Leyton, Gregory W. Moseley, David A. Jans Source Type: journals

Immunization with a DNA vaccine candidate in chronic hepatitis C patients is safe, well tolerated and does not impair immune response induction after anti-hepatitis B vaccinationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present study, we evaluated the safety of CIGB-230, a novel vaccine candidate based on the mixture of a plasmid for DNA immunization, expressing hepatitis C virus (HCV) structural antigens, with a recombinant HCV Core protein.Fifteen HCV chronically-infected volunteers with detectable levels of HCV RNA genotype 1b, who were nonresponders to previous treatment with interferon plus ribavirin, were intramuscularly injected with CIGB-230 on weeks 0, 4, 8, 12, 16 and 20. Individuals were also immunized at weeks 28, 32 and 36 with a recombinant vaccine against hepatitis B. Adverse events were recorded and analyzed. Blood ...
Source: The Journal of Gene Medicine - October 28, 2009 Category: Genetics & Stem Cells Authors: Marlen Castellanos, Zurina Cinza, Zaily Dorta, Gloria Veliz, Héctor Vega, Irma Lorenzo, Sergio Ojeda, Santiago Dueñas-Carrera, Liz Alvarez-Lajonchere, Gillian Martínez, Elena Ferrer, Miladys Limonta, Marbelis Linares, Odalis Ruiz, Boris Acevedo, Dinora Source Type: journals

Synergistic therapeutic effects of combined adenovirus-mediated interleukin-10 and interleukin-12 gene therapy on airway inflammation in asthmatic miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Asthma is a chronic disease characterized by airway inflammation caused by the dysregulated production of cytokines secreted by allergen-specific type 2 T helper (Th2) cells. Although the Th1-promoting cytokine, interleukin (IL)-12, is capable of inhibiting Th2-driven allergen-induced airway changes in mice, IL-12 also aggravates the Th1-driven inflammatory pulmonary pathology. Further, IL-10 was found to exert both anti-inflammatory and immunoregulatory activities. To avoid the side-effects of IL-12, we hypothesized that the low-dose expression of IL-10 with concomitant IL-12 administration in the airway may represent a m...
Source: The Journal of Gene Medicine - October 28, 2009 Category: Genetics & Stem Cells Authors: Chih-Yu Hsu, H. Eugene Liu, Feei-Yi Sheu, Sy-Jye Leu, Bor-Luen Chiang, George Hsiao, Yueh-Lun Lee Source Type: journals

Combination of alpha-1 antitrypsin and doxycycline suppresses collagen-induced arthritisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rheumatoid arthritis (RA) is a complex disease characterized by autoimmune inflammation and joint destruction. Despite recent advances in RA treatment, current therapies require further improvement to overcome adverse events and ineffectiveness in some cases. By targeting different pathways/molecules using drug combinations, a better treatment can be obtained, whereas adverse events are reduced. In order to develop a new treatment option, the present study employs a gene therapy-based combination therapy using doxycycline and human alpha-1 antitrypsin (hAAT).DBA/1 mice were immunized with type II collagen to induce arthrit...
Source: The Journal of Gene Medicine - October 28, 2009 Category: Genetics & Stem Cells Authors: Christian Grimstein, Young-Kook Choi, Minoru Satoh, Yuanqing Lu, Xueying Wang, Martha Campbell-Thompson, Sihong Song Source Type: journals

Multi-armed poly(L-glutamic acid)-graft-oligoethylenimine copolymers as efficient nonviral gene delivery vectorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The application of polyethylenimine (PEI) in gene delivery has been severely limited by significant cytotoxicity that results from a nondegradable methylene backbone and high cationic charge density. It is therefore necessary to develop novel biodegradable PEI derivates for low-toxic, highly efficient gene delivery.A series of novel cationic copolymers with various charge density were designed and synthesized by grafting different kinds of oligoethylenimine (OEI) onto a determinate multi-armed poly(L-glutamic acid) backbone. The molecular structures of multi-armed poly(L-glutamic acid)-graft-OEI (MP-g-OEI) copolymers were ...
Source: The Journal of Gene Medicine - October 19, 2009 Category: Genetics & Stem Cells Authors: Lei Chen, Huayu Tian, Jie Chen, Xuesi Chen, Yubin Huang, Xiabin Jing Source Type: journals

Selective tropism of the recombinant adeno-associated virus 9 serotype for rat cardiac tissueEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cardiac gene transfer may serve as a novel therapeutic approach for heart disease. Numerous serotypes of recombinant adeno-associated virus (rAAV) have been identified with variable tropisms to cardiac tissue.Both in vitro and in vivo experiments were undertaken to compare cardiac tropisms of rAAV-2, 5, 7, 8 and 9. For the in vitro studies, 107 vector genome (vg) of rAAV-2, 5, 7, 8 or 9 were used to transduce both rat neonatal cardiac myocytes (RNCM) and fibroblasts (RNCF). For the in vivo studies, 4 × 1010 vg of rAAV-2, 5, 7, 8 or 9, and 4 × 1011 vg of rAAV8 or 9 were administered in 5-day-old rats via a relatively non-...
Source: The Journal of Gene Medicine - October 14, 2009 Category: Genetics & Stem Cells Authors: Yanfei Qi, Xuan Liu, Hongwei Li, Vinayak Shenoy, Qiuhong Li, William W. Hauswirth, Colin Sumners, Michael J. Katovich Source Type: journals

Controlled systemic release of interleukin-12 after gene electrotransfer to muscle for cancer gene therapy alone or in combination with ionizing radiation in murine sarcomasEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study aimed to evaluate the antitumor effectiveness of systemic interleukin (IL)-12 gene therapy in murine sarcoma models, and to evaluate its interaction with the irradiation of tumors and metastases. To avoid toxic side-effects of IL-12 gene therapy, the objective was to achieve the controlled release of IL-12 after intramuscular gene electrotransfer.Gene electrotransfer of the plasmid pORF-mIL12 was performed into the tibialis cranialis in A/J and C57BL/6 mice. Systemic release of the IL-12 was monitored in the serum of mice after carrying out two sets of intramuscular IL-12 gene electrotransfer of two diffe...
Source: The Journal of Gene Medicine - September 22, 2009 Category: Genetics & Stem Cells Authors: Gregor Tevz, Simona Kranjc, Maja Cemazar, Urska Kamensek, Andrej Coer, Mojca Krzan, Suzana Vidic, Darja Pavlin, Gregor Sersa Source Type: journals

Engineering adeno-associated virus serotype 2-based targeting vectors using a new insertion site-position 453-and single point mutationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Genetic modification of capsid proteins by peptide insertion has created the possibility of using adeno-associated viral (AAV) vectors for receptor specific gene transfer (AAV targeting). The most common site used for insertion in AAV serotype 2 capsids are amino acid positions 587 and 588 located at the second highest capsid protrusion. Reasoning that peptide insertions at the most exposed position augments target receptor interaction, we explored position 453 as a new insertion site.Position 453 was identified in silico. Capsid mutants carrying the model ligand RGD-4C in position 453 with and without R585A/R588A substitu...
Source: The Journal of Gene Medicine - September 22, 2009 Category: Genetics & Stem Cells Authors: Jorge Boucas, Kerstin Lux, Anke Huber, Stephanie Schievenbusch, Miriam John von Freyend, Luca Perabo, Sibille Quadt-Humme, Margarete Odenthal, Michael Hallek, Hildegard Büning Source Type: journals

Mathematical modelling of the impact of haematopoietic stem cell-delivered gene therapy for HIVEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Gene therapy represents a new treatment paradigm for HIV that is potentially delivered by a safe, once-only therapeutic intervention.Using mathematical modelling, we assessed the possible impact of autologous haematopoietic stem cell (HSC) delivered, anti-HIV gene therapy. The therapy comprises a ribozyme construct (OZ1) directed to a conserved region of HIV-1 delivered by transduced HSC (OZ1+HSC). OZ1+HSC contributes to the CD4+ T lymphocyte and monocyte/macrophage cell pools that preferentially expand under the selective pressure of HIV infection. The model was used to predict the efficacy of OZ1 in a highly active antir...
Source: The Journal of Gene Medicine - September 22, 2009 Category: Genetics & Stem Cells Authors: John M. Murray, Greg C. Fanning, Janet L. Macpherson, Louise A. Evans, Susan M. Pond, Geoff P. Symonds Source Type: journals

Identification of the murine AAVrh32.33 capsid-specific CD8+ T cell epitopesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Adeno-associated virus (AAV) is an ideal gene therapy vector and is non-immunogenic in many small animal models. The stable gene expression commonly seen in murine models does not necessarily translate to nonhuman primates and higher-order species, highlighting the need for a better understanding of immune activation to these vectors. One capsid variant, AAVrh32.33, demonstrates a unique phenotype in murine muscle, reminiscent of what is often seen in higher-order species. AAVrh32.33 generates a strong CD8+ T-cell response to both capsid and encoded transgene antigens in a manner independent of transgene product or major h...
Source: The Journal of Gene Medicine - September 22, 2009 Category: Genetics & Stem Cells Authors: Lauren E. Mays, James M. Wilson Source Type: journals

Efficient gene delivery into mammalian cells by recombinant baculovirus containing a hybrid cytomegalovirus promoter/Semliki Forest virus repliconEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Baculovirus, which is widely utilized as an excellent tool for the production of recombinant protein in insect cells, has recently emerged as a novel and attractive gene delivery vehicle for mammalian cells. Alphavirus, such as Semliki Forest virus (SFV), has also received considerable attention for use as expression vectors because of its self-replicating property. In the present study, we investigated the characterization of recombinant baculovirus incorporating a hybrid cytomegalovirus (CMV) promoter/SFV replicon.Recombinant baculovirus containing the hybrid CMV promoter/SFV replicon was constructed. Using enhanced gree...
Source: The Journal of Gene Medicine - September 14, 2009 Category: Genetics & Stem Cells Authors: Yongfei Pan, Qian Zhao, Liurong Fang, Rui Luo, Huanchun Chen, Shaobo Xiao Source Type: journals

Liver-restricted expression of the canine factor VIII gene facilitates prevention of inhibitor formation in factor VIII-deficient miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Gene therapy for hemophilia A with adeno-associated virus (AAV) vectors involves difficulties in the efficient expression of factor VIII (FVIII) and in antibody formation against transgene-derived FVIII.AAV8 vectors carrying the canine B domain deleted FVIII (cFVIII) gene under the control of the ubiquitous [beta]-actin promoter, the liver-specific human [alpha]1 anti-trypsin promoter (HAAT) and the liver-specific hepatic control region (HCR) enhancer/human [alpha]1 anti-trypsin promoter complex (HCRHAAT) were used for the expression of cFVIII in FVIII deficient (fviii-/-) mice.Addition of the hepatic control region enhanc...
Source: The Journal of Gene Medicine - September 14, 2009 Category: Genetics & Stem Cells Authors: Akira Ishiwata, Jun Mimuro, Hiroaki Mizukami, Yuji Kashiwakura, Katsuhiro Takano, Tsukasa Ohmori, Seiji Madoiwa, Keiya Ozawa, Yoichi Sakata Source Type: journals

Targeting of plasmid DNA-lipoplexes to cells with molecules anchored via a metal chelator lipidEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The ability to deliver plasmid DNA (pDNA) to specific cells in vivo is crucial for achieving efficient targeted transfection with nonviral vectors. We previously used stealth liposomes containing the chelator lipid 3(nitrilotriacetic acid)-ditetradecylamine (NTA3-DTDA) to target delivery of antigen and cytokines to immune cells in vivo. In the present study, we utilized liposomes containing NTA3-DTDA and the ionizable aminolipid 1,2-dioleoyl-3-dimethyl-ammonium-propane (DODAP) to incorporate pDNA into complexes for targeting to cells.Liposomes containing DODAP, NTA3-DTDA and helper lipids were acidified (pH 5.5) and mixed ...
Source: The Journal of Gene Medicine - September 14, 2009 Category: Genetics & Stem Cells Authors: Thomas P. Herringson, Ram R. Patlolla, Joseph G. Altin Source Type: journals

Evaluation of the muscle gene transfer activity of a series of amphiphilic triblock copolymersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Amphiphilic triblock copolymers such as the polyethylene oxide-polypropylene oxide-polyethylene oxide L64 (PEO13-PPO30-PEO13) significantly increase transgene expression after injection of DNA/polymer mixtures into skeletal muscles. To better understand the way such copolymers act, we studied the behaviour of different poloxamers, including L64, both in vitro and in vivo.The in vitro and in vivo transfection activity of five copolymers that differ either by their molecular weight or by their hydrophilic/hydrophobic balance was evaluated. Furthermore, we also studied the membrane permeabilizing properties of the poloxamers....
Source: The Journal of Gene Medicine - September 14, 2009 Category: Genetics & Stem Cells Authors: Debborah Alimi-Guez, Christian Leborgne, Gaelle Pembouong, Laetitia Van Wittenberghe, Nathalie Mignet, Daniel Scherman, Antoine Kichler Source Type: journals

Efficient adenovirus-mediated gene transfer to gastric tissue by oral administrationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recombinant adenoviruses (rAd) are well-characterized viral vectors and have been studied in many human diseases. However, there are no detailed methods for transferring genes to the stomach using rAd.Gastric epithelial cells were infected with rAd encoding green fluorescence protein (AdGFP) for different times, or with AdGFP that had been incubated in artificial gastric juice at different pH values for 1 h. Gene expression was detected by fluorescence microscope and flow cytometry. Mice were infected via oral administration with rAd encoding red fluorescence protein and [beta]-galactosidase (AdRFP-lacZ) or rAd encoding mo...
Source: The Journal of Gene Medicine - September 14, 2009 Category: Genetics & Stem Cells Authors: Xiao-Fei Liu, Yun Shi, Jin-Yu Zhang, Yuan Zhuang, Ke-Ran Jia, Xu-Hu Mao, Ying Guo, Tao Liu, Zhen Liu, Chao Wu, Wei-Jun Zhang, Wei-Ying Zhou, Gang Guo, Quan-Ming Zou Source Type: journals

A lentiviral vector-based adenovirus fiber-pseudotyping approach for expedited functional assessment of candidate retargeted fibersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Many studies aimed at retargeting adenovirus (Ad) rationally focus on genetic modification of fiber, which is the primary receptor-binding protein of Ad. Retargeted fibers ultimately require functional validation in the viral context.Lentiviral vectors (LV) were used to express fiber variants in cells. Infections with a fiber gene-deleted Ad vector yielded fiber-pseudotyped viruses. An enzyme-linked immunosorbent assay and slot blot-based assays probed target binding-ability of retargeted fibers. Differential treatments with an alkylating agent prior to western blot analysis allowed for examination of intra- and extracellu...
Source: The Journal of Gene Medicine - September 14, 2009 Category: Genetics & Stem Cells Authors: Taco G. Uil, Jeroen de Vrij, Jort Vellinga, Martijn J. W. E. Rabelink, Steve J. Cramer, On Ying A. Chan, Margherita Pugnali, Maria Magnusson, Leif Lindholm, Pierre Boulanger, Rob C. Hoeben Source Type: journals

Enhanced immunogenicity of an anti-caries vaccine encoding a cell-surface protein antigen of Streptococcus mutans by intranasal DNA prime-protein boost immunizationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The present study aimed to enhance the specific anti-caries immunity induced by DNA prime-protein boost strategy for an A-P fragment of a cell-surface protein antigen of Streptococcus mutans (PAc).BALB/c mice were immunized with DNA prime-protein boost, DNA-DNA or protein-protein regimens by the intranasal route, using combinations of plasmid vector (pCIA-P) that express PAc protein and a pure secretec recombinant PAc protein (rPAc). Then, a gnotobiotic mouse model was constructed 2 weeks after the last immunization, and specific immune responses in vivo and their protection against dental caries were observed.The present ...
Source: The Journal of Gene Medicine - August 28, 2009 Category: Genetics & Stem Cells Authors: Yuhong Li, Jie Jin, Yaping Yang, Zhuan Bian, Zhi Chen, Mingwen Fan Source Type: journals

FK506 as an adjuvant of tolerogenic DNA vaccination for the prevention of experimental autoimmune encephalomyelitisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
DNA vaccination is a strategy that has been developed primarily to elicit protective immunity against infection and cancer.DNA vaccine was used, in conjunction with an immunosuppressant, to tolerize harmful autoimmunity.Immunization of C57BL/6 mice with MOG35-55, a myelin oligodendrocyte glycoprotein-derived peptide, and FK506 (Tacrolimus) as a tolerogenic adjuvant stimulated regulatory dendritic cells, induced antigen-specific regulatory T cells (Treg), and protected the animals from subsequent induction of experimental autoimmune encephalomyelitis (EAE). After EAE induction, there were fewer lymphocytes, including fewer ...
Source: The Journal of Gene Medicine - August 17, 2009 Category: Genetics & Stem Cells Authors: Youmin Kang, Jia Zhao, Yue Liu, Aoshuang Chen, Guoxing Zheng, Yang Yu, Jianjie Mi, Qiang Zou, Bin Wang Source Type: journals

A chimeric adenovirus with an Ad 3 fiber knob modification augments glioma virotherapyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Malignant gliomas remain refractory to treatment despite advances in chemotherapy and surgical techniques. Viral vectors developed to treat gliomas have had low transduction capabilities, limiting their use. Gliomas over-express CD46, CD80, and CD86, all of which bind adenovirus serotype 3.To increase the infectivity and replication of oncolytic vectors in malignant brain tumors, we created a conditionally replicating adenovirus, CRAd-Survivin-5/3, which contains a survivin promoter-driving E1A and a chimeric fiber consisting of adenovirus serotype 3 knob.In vitro, this modified CRAd showed ten- to 100-fold increased cytot...
Source: The Journal of Gene Medicine - August 16, 2009 Category: Genetics & Stem Cells Authors: Suvobroto Nandi, Ilya V. Ulasov, Cleo E. Rolle, Yu Han, Maciej S. Lesniak Source Type: journals

Systemic adenoviral gene delivery to orthotopic murine breast tumors with ablation of coagulation factors, thrombocytes and Kupffer cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rapid clearance of adenoviruses from blood by macrophage lineage cells of the liver and spleen, and binding to platelets, hinder their successful systemic use for cancer gene therapy. Vitamin K dependent coagulation factors are important mediators for the adenovirus liver tropism. Here we aim to determine the effects of coagulation factor, thrombocyte and liver macrophage (Kupffer cell) ablation on biodistribution of serotype 5 adenoviruses in mice with orthotopic breast tumors.Prior to intravenous injection of adenoviruses, mice bearing orthotopic breast tumors were pretreated with warfarin to inhibit vitamin K dependent ...
Source: The Journal of Gene Medicine - August 10, 2009 Category: Genetics & Stem Cells Authors: Anniina Koski, Maria Rajecki, Kilian Guse, Anna Kanerva, Ari Ristimäki, Sari Pesonen, Sophie Escutenaire, Akseli Hemminki Source Type: journals

Purification of adenoviral vectors by combined anion exchange and gel filtration chromatographyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present a simple two-step chromatography process that is capable of producing high-quality adenovirus at a titre suitable for scale-up and clinical translation. Copyright © 2009 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - August 7, 2009 Category: Genetics & Stem Cells Authors: Marc N. Eglon, Aoife M. Duffy, Timothy O'Brien, Padraig M. Strappe Source Type: journals

Correction of mutant Fanconi anemia gene by homologous recombination in human hematopoietic cells using adeno-associated virus vectorEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined an ability of AAV vectors to repair a mutation in human hematopoietic cells by HR.We infected a human B-lymphoblastoid cell line (BCL) derived from a normal subject with an AAV, which disrupts the hypoxanthine phosphoribosyl transferase1 (HPRT1) locus, to measure the frequency of AAV-mediated HR in BCL cells. We subsequently constructed an AAV vector encoding the normal sequences from the Fanconi anemia group A (FANCA) locus to correct a mutation in the gene in BCL derived from a FANCA patient.Under optimal conditions, approximately 50% of BCL cells were transduced with an AAV serotype 2 (AAV-2) vector. In FANC...
Source: The Journal of Gene Medicine - August 3, 2009 Category: Genetics & Stem Cells Authors: Kittiphong Paiboonsukwong, Fumi Ohbayashi, Haruka Shiiba, Emi Aizawa, Takayuki Yamashita, Kohnosuke Mitani Source Type: journals

Immobilized HIV-1 Tat protein promotes gene transfer via a transactivation-independent mechanism which requires binding of Tat to viral particlesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Retroviral transduction of cells is improved upon virus adsorption onto immobilized fibronectin (FN) fragments. Because HIV-1 Tat possesses the same functional domains that lead to increased transduction efficiency in FN by colocalization of bound virus and cells, we hypothesized that Tat could enhance gene transfer by a similar mechanism.Single-cycle replication retro- or lentivirus carrying green fluorescent protein or cloramphenicol acetyltransferase as reporter genes were added to wells coated with Tat or Tat peptides. Wells were extensively washed to remove unbound virus and levels of transduction were detected by mea...
Source: The Journal of Gene Medicine - August 2, 2009 Category: Genetics & Stem Cells Authors: Filomena Nappi, Chiara Chiozzini, Valentina Bordignon, Alessandra Borsetti, Stefania Bellino, Marco Cippitelli, Giovanni Barillari, Antonella Caputo, Mudit Tyagi, Mauro Giacca, Barbara Ensoli Source Type: journals

Reversible neurochemical changes mediated by delayed intrastriatal glial cell line-derived neurotrophic factor gene delivery in a partial Parkinson's disease rat modelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Efficient protection of dopaminergic neurons against a subsequent 6-hydroxydopamine lesion by glial cell line-derived neurotrophic factor (GDNF) gene delivery has been demonstrated. By contrast, the neurorestorative effects of GDNF administered several weeks after the toxin have been less characterized. In particular, whether these were permanent or dependent on the continuous presence of GDNF remains elusive.A tetracycline-inducible adeno-associated virus (AAV)-1 vector expressing human GDNF cDNA was administered unilaterally in the rat striatum 5 weeks after 6-hydroxydopamine. Rats were treated with doxycycline (dox) or ...
Source: The Journal of Gene Medicine - July 28, 2009 Category: Genetics & Stem Cells Authors: Xin Yang, Birgit Mertens, Enni Lehtonen, Linda Vercammen, Olivier Bockstael, Abdelwahed Chtarto, Marc Levivier, Jacques Brotchi, Yvette Michotte, Veerle Baekelandt, Sophie Sarre, Liliane Tenenbaum Source Type: journals

Differences in gene expression between sonoporation in tumor and in muscleEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ultrasound (US) is a novel and effective tool for the local delivery of genes into target tissues. US can temporarily change the permeability of a cell membrane and thus enhance the delivery of naked DNA into cells. In the present study, the efficiencies of gene expression mediated by US delivery in orthotopic liver tumor, subcutaneous tumor and muscle tissue were evaluated by changing the contrast agent concentrations and US exposure durations.Plasmid DNA coding for luciferase, interleukin-12 or enhanced green fluorescence protein was mixed with SonoVue® and injected intratumorally or intramuscularly. The injection sites...
Source: The Journal of Gene Medicine - July 27, 2009 Category: Genetics & Stem Cells Authors: Kun-Che Tsai, Zhe-Kang Liao, Shu-Jyuan Yang, Win-Li Lin, Ming-Jium Shieh, Lih-Hwa Hwang, Wen-Shiang Chen Source Type: journals

Single-dose lentiviral gene transfer for lifetime airway gene expressionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cystic fibrosis (CF) is caused by a defect in cystic fibrosis transmembrane conductance regulator (CFTR) activity, often resulting in an incurable airway disease. Gene therapy into the conducting airway epithelium is a potential cure for CF; however, most gene vectors do not result in long-lived expression, and require re-dosing. Perversely, intrinsic host immune responses can then block renewed gene transfer.To investigate whether persistent gene expression could be achieved after a single dosing event, thus avoiding the issue of blocking host responses, we used a gene transfer protocol that combined an airway pretreatmen...
Source: The Journal of Gene Medicine - July 26, 2009 Category: Genetics & Stem Cells Authors: Alice G. Stocker, Karlea L. Kremer, Rachel Koldej, Darren S. Miller, Donald S. Anson, David W. Parsons Source Type: journals

Alkylcarboxylate grafting to polyethylenimine: a simple approach to producing a DNA nanocarrier with low toxicityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Various strategies have been examined to improve both transfection efficiency and cytotoxicity of polyethylenimine (PEI), a widely used polycationic nonviral gene vector. In the present study, we sought to improve PEI transfection efficiency by combining the osmotic burst mechanism for lysing endocytotic vesicles with the lipid depletion mechanism, which was accomplished by maintaining buffering capacity at the same time as adding a lipid-absorbing hydrophobic shell.PEI was altered via the substitution of various percentages of its primary amines with carboxylate-terminated short, moderate and long alkyl chains, by reactio...
Source: The Journal of Gene Medicine - July 24, 2009 Category: Genetics & Stem Cells Authors: Reza K. Oskuee, Ali Dehshahri, Wayne T. Shier, Mohammad Ramezani Source Type: journals

Stem cell antigen-1+ cell-based bone morphogenetic protein-4 gene transfer strategy in mice failed to promote endosteal bone formationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study assessed whether a Sca-1+ cell-based ex vivo gene transfer strategy, which has been shown to promote robust endosteal bone formation with a modified fibroblast growth factor-2 (FGF2) gene, can be extended to use with bone morphogenetic protein (BMP)2/4 hybrid gene.Sublethally irradiated recipient mice were transplanted with lentiviral (LV)-BMP2/4-transduced Sca-1+ cells. Bone parameters were monitored by pQCT and µCT. Gene expression was assessed by the real-time reverse transcriptase-polymerase chain reaction.Recipient mice of LV-BMP2/4-transduced Sca-1+ cells yielded high engraftment and increased BMP4 mRNA l...
Source: The Journal of Gene Medicine - July 22, 2009 Category: Genetics & Stem Cells Authors: Susan L. Hall, Shin-Tai Chen, Reinhard Gysin, Daila S. Gridley, Subburaman Mohan, K.-H. William Lau Source Type: journals

Impaired clearance of accumulated lysosomal glycogen in advanced Pompe disease despite high-level vector-mediated transgene expressionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Infantile-onset glycogen storage disease type II (GSD-II; Pompe disease; MIM 232300) causes death early in childhood from cardiorespiratory failure in the absence of effective treatment, whereas late-onset Pompe disease causes a progressive skeletal myopathy. The limitations of enzyme replacement therapy could potentially be addressed with adeno-associated virus (AAV) vector-mediated gene therapy.AAV vectors containing tissue-specific regulatory cassettes, either liver-specific or muscle-specific, were administered to 12- and 17-month-old Pompe disease mice to evaluate the efficacy of gene therapy in advanced Pompe disease...
Source: The Journal of Gene Medicine - July 20, 2009 Category: Genetics & Stem Cells Authors: Baodong Sun, Haoyue Zhang, Andrew Bird, Songtao Li, Sarah P. Young, Dwight D. Koeberl Source Type: journals

Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension culturesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Lentiviral vectors (LV) offer several advantages over other gene delivery vectors. Their potential for the integration and long-term expression of therapeutic genes renders them an interesting tool for gene and cell therapy interventions. However, large-scale LV production remains an important challenge for the translation of LV-based therapeutic strategies to the clinic. The development of robust processes for mass production of LV is needed.A suspension-grown HEK293 cell line was exploited for the production of green fluorescent protein-expressing LV by transient polyethylenimine (PEI)-based transfection with LV-encoding...
Source: The Journal of Gene Medicine - July 19, 2009 Category: Genetics & Stem Cells Authors: Sven Ansorge, Stéphane Lanthier, Julia Transfiguracion, Yves Durocher, Olivier Henry, Amine Kamen Source Type: journals

Modification of dendritic cells with interferon-[gamma]-inducible protein-10 gene to enhance vaccine potencyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dendritic cell (DC)-based vaccines have become a promising modality in cancer immunotherapy. However, their ability to initiate tumor antigen-specific T cell immunity is limited in various negative-feedback mechanisms. The rapid down-regulation of chemokines, such as the interferon inducible protein of 10 kDa (IP-10), which chemoattracts activated antigen-specific CD8+ T cells, would represent negative-feedback regulation. Therefore, we attempted to improve DC vaccine potency by introducing the IP-10 gene retrovirally aiming to replenish the chemoattractive activity of DCs.We introduced IP-10 gene into DC2.4 cells, referre...
Source: The Journal of Gene Medicine - July 19, 2009 Category: Genetics & Stem Cells Authors: Tae Heung Kang, Hyun Cheol Bae, Seok-Ho Kim, Su Hong Seo, Sang Wook Son, Eun Young Choi, Seung-Yong Seong, Tae Woo Kim Source Type: journals

Intracellular small interfering RNA delivery using genetically engineered double-stranded RNA binding protein domainEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A variety of synthetic carriers, such as cationic polymers and lipids, have been used as nonviral carriers for small interfering RNA (siRNA) delivery. Although siRNA polyplexes and lipoplexes exhibited good gene silencing efficiencies, they often showed serious cytotoxicities, which are not useful for clinical applications. A double-stranded RNA binding cellular protein with highly specific siRNA binding property and noncytotoxicity was used for siRNA delivery.A double-stranded RNA binding domain (dsRBD) of human double-stranded RNA activated protein kinase R was genetically produced and utilized to complex siRNA for intra...
Source: The Journal of Gene Medicine - June 29, 2009 Category: Genetics & Stem Cells Authors: Juwon Kim, Soo Hyeon Lee, Joonho Choe, Tae Gwan Park Source Type: journals

Hyaluronic acid complexed to biodegradable poly L-arginine for targeted delivery of siRNAsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Small interfering RNA (siRNA) has been recognized as a new therapeutic drug to treat various diseases by inhibition of oncogene or viral gene expression. Because hyaluronic acid (HA) has been described as a biocompatible biomaterial, we tested the nanoparticles formed by electrostatic complexation of negatively-charged HA and cationic poly L-arginine (PLR) for siRNA delivery systems.Different electrostatic complexes of HA and PLR (HPs) were formulated: HP101 with 50% (w/w) HA and HP110 with 9% (w/w) HA.Gel retardation assays showed that HP101 and HP110 could form complexes with siRNAs. The diameters of these complexes were...
Source: The Journal of Gene Medicine - June 29, 2009 Category: Genetics & Stem Cells Authors: Eun-Joong Kim, Gayong Shim, Kwangmyeung Kim, Ick Chan Kwon, Yu-Kyoung Oh, Chang-Koo Shim Source Type: journals

A systematic study of the function of the human [beta]-globin introns on the expression of the human coagulation factor IX in cultured Chinese hamster ovary cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Intronic sequences have the potential to improve gene expression in eukaryotes by a variety of mechanisms. In this context, human [beta]-globin (hBG) introns were inserted into the human factor IX (hFIX) cDNA in cytomegalovirus (CMV)-regulated plasmids. The resulting construct was then used for further expression analysis in vitro.Seven hFIX-expressing plasmids with different combinations of the two hBG introns and the Kozak element were constructed and used for a systematic expression analysis in cultured Chinese hamster ovary (CHO) cells. In parallel, the hBG intronic sequences were analysed for the presence of possible ...
Source: The Journal of Gene Medicine - June 28, 2009 Category: Genetics & Stem Cells Authors: Aliakbar Haddad-Mashadrizeh, Alireza Zomorodipour, Mehrnaz Izadpanah, Mohammad Reza Sam, Fariba Ataei, Farzaneh Sabouni, Seyed Javad Hosseini Source Type: journals

Chemokine-derived peptides as carriers for gene delivery to CXCR4 expressing cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cell and tissue-specific DNA delivery can be achieved by derivatizing vehicles with a targeting ligand for certain receptor. CXCR4 is a receptor of chemokine stromal cell-derived factor (SDF)-1 and viral protein viral macrophage inflammatory protein (vMIP)-II. It is expressed on some types of stem and cancer cells. The present study aimed to design and characterize the group of CXCR4 targeted peptides for receptor-mediated gene delivery. We focused on bifunctional peptide carriers: two derived from N-terminal sequences of SDF-1 and one from vMIP-II.Three synthetic chemokine-derived peptides, designated long CDP (KPVSLSYRSP...
Source: The Journal of Gene Medicine - June 25, 2009 Category: Genetics & Stem Cells Authors: Anna Egorova, Anton Kiselev, Marika Hakli, Marika Ruponen, Vladislav Baranov, Arto Urtti Source Type: journals

Antifibrotic effect through the regulation of transcription factor using ring type-Sp1 decoy oligodeoxynucleotide in carbon tetrachloride-induced liver fibrosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Liver fibrosis is characterized by the excessive accumulation of extracellular matrix (ECM). Recent advances in the knowledge about the cellular, molecular and genetic aspects of fibrosis have opened a new era of research on liver cirrhosis. A transcription factor, Sp1, originally described as a ubiquitous transcription factor, is involved in the basal expression of ECM genes and may be important in the fibrotic processes.The chronic hepatic damage received intraperitoneal injection of carbon tetrachloride (2 mg/kg) dissolved in corn oil (1 : 3 ratio) three times a weekly for 8 weeks. The delivery of decoy oligodeoxynucleo...
Source: The Journal of Gene Medicine - June 24, 2009 Category: Genetics & Stem Cells Authors: Ji-Hyun Park, Ji-Ho Jo, Kyung-Hyun Kim, Soo-Jung Kim, Woo-Ram Lee, Kwan-Kyu Park, Jae-Bok Park Source Type: journals

Bipartite vectors for co-expression of a growth factor cDNA and short hairpin RNA against an apoptotic geneEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Although human islet transplantation is a promising approach for treating type I diabetes, its success is limited as a result of the poor survival rate of transplanted islets. Expression of a growth factor gene to promote revascularization and silencing of pro-apoptotic genes before transplantation may improve the outcome of islet transplantation.In the present study, we constructed bipartite plasmid vectors to co-express a vascular endothelial growth factor (VEGF) cDNA and short hairpin (sh)RNA targeting inducible NO synthase (iNOS) gene. First, we screened shRNA sequences against human iNOS by transfecting plasmids encod...
Source: The Journal of Gene Medicine - June 23, 2009 Category: Genetics & Stem Cells Authors: Feng Li, Ram I. Mahato Source Type: journals

Neuroprotection in a 6-hydroxydopamine-lesioned Parkinson model using lactoferrin-modified nanoparticlesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nonviral gene therapy of chronic degenerative diseases such as Parkinson's disease (PD) is a great challenge as a result of the low tranfection efficiency of nonviral gene vectors. We previously constructed a lactoferrin (Lf)-modified vector, which was demonstrated to be potential for brain gene delivery both in vitro and in vivo. In the present study, this type of vector was applied to load human glial cell line-derived neurotrophic factor gene (hGDNF).A rat PD model was constructed by the unilateral lesion of striatum using 6-hydroxydopamine (6-OHDA). Lf-modified nanoparticles (NPs) were prepared and characterized. Neuro...
Source: The Journal of Gene Medicine - June 23, 2009 Category: Genetics & Stem Cells Authors: Rongqin Huang, Liang Han, Jianhua Li, Feiliang Ren, Weilun Ke, Chen Jiang, Yuanying Pei Source Type: journals

Combining angiogenic gene and stem cell therapies for myocardial infarctionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Transplantation of stem cells from various sources into infarcted hearts has the potential to promote myocardial regeneration. However, the regenerative capacity is limited partly as a result of the low survival rate of the transplanted cells in the ischemic myocardium. In the present study, we tested the hypothesis that combining cell and angiogenic gene therapies would provide additive therapeutic effects via co-injection of bone marrow-derived mesenchymal stem cells (MSCs) with an adeno-associated viral vector (AAV), MLCVEGF, which expresses vascular endothelial growth factor (VEGF) in a cardiac-specific and hypoxia-ind...
Source: The Journal of Gene Medicine - June 23, 2009 Category: Genetics & Stem Cells Authors: Jennifer Pons, Yu Huang, Junya Takagawa, Janice Arakawa-Hoyt, Jianqin Ye, William Grossman, Yuet Wai Kan, Hua Su Source Type: journals

Specific inhibition of epithelial Na+ channels by antisense oligonucleotides for the treatment of Na+ hyperabsorption in cystic fibrosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cystic fibrosis (CF) respiratory epithelia are characterized by a defect Cl- secretion and an increased Na+ absorption through epithelial Na+ channels (ENaC). The present study aimed to find an effective inhibitor of human ENaC with respect to replacing amiloride therapy for CF patients. Therefore, we developed specific antisense oligonucleotides (AON) that efficiently suppress Na+ hyperabsorption by inhibiting the expression of the [alpha]-ENaC subunit.We heterologously expressed ENaC in oocytes of Xenopus laevis for mass screening of AON. Additionally, primary cultures of human nasal epithelia were transfected with AON a...
Source: The Journal of Gene Medicine - June 15, 2009 Category: Genetics & Stem Cells Authors: Katja Sobczak, Andrei Segal, Nadine Bangel-Ruland, Judith Semmler, Willy Van Driessche, Hermann Lindemann, Ralf Heermann, Wolf-Michael Weber Source Type: journals

Immunogenicity of intrathecal plasmid gene delivery: cytokine release and effects on transgene expressionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
One method for the delivery of therapeutic proteins to the spinal cord is to inject nonviral gene vectors including plasmid DNA into the cerebrospinal fluid (CSF) that surrounds the spinal cord (intrathecal space). This approach has produced therapeutic benefits in animal models of disease and several months of protein expression; however, there is little information available on the immune response to these treatments in the intrathecal space, the relevance of plasmid CpG sequences to any plasmid-induced immune response, or the effect of this immune response on transgene expression.In the present study, coding or noncodin...
Source: The Journal of Gene Medicine - June 15, 2009 Category: Genetics & Stem Cells Authors: Travis S. Hughes, Stephen J. Langer, Salla I. Virtanen, Raymond A. Chavez, Linda R. Watkins, Erin D. Milligan, Leslie A. Leinwand Source Type: journals

The deployment of adenovirus-containing gene activated matrices onto severed axons after central nervous system injury leads to transgene expression in target neuronal cell bodiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In previous studies, we showed that gene activated matrices (GAMs) containing nonviral vectors successfully deliver genes to neurons after optic nerve and spinal cord injury. In the present study, we evaluated whether adenoviral vectors delivered within a GAM increase the efficiency of local gene delivery to injured CNS neurons. Lyophilized GAMs containing collagen and adenoviral vectors were assessed in vitro and in vivo.We evaluated viral vector stability, release kinetics and efficiency of transduction for this GAM formulation in vitro using the quantitative polymerase chain reaction (qPCR), flow cytometry and fluoresce...
Source: The Journal of Gene Medicine - June 9, 2009 Category: Genetics & Stem Cells Authors: Ana Maria Gonzalez, Oscar Berlanga, Wendy E. Leadbeater, Lisa Cooper-Charles, Karen Sims, Ann Logan, Brian Eliceiri, Martin Berry, Andrew Baird Source Type: journals

Clinical grade preparation of human natural regulatory T-cells encoding the thymidine kinase suicide gene as a safety gene: authors' reponseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This Response refers to JGM 1286, which was published in The Journal of Gene Medicine 11:2. Please follow the link below to view the Letter to the Editor. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - June 8, 2009 Category: Genetics & Stem Cells Authors: Maude Guillot-Delost, Mustapha Cheraï, Yamina Hamel, Michelle Rosenzwajg, Claude Baillou, Ghislaine Simonin, Virginie Leclercq, Maria Encarnita Mariotti-Ferrandiz, Adrien Six, Véronique Bon-Durand, Sébastien Maury, Benoit L. Salomon, José L. Cohen, Da Source Type: journals

Characterization of an alternative packaging system derived from the cat RD114 retrovirus for gene deliveryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe and characterize an alternative retroviral packaging system derived from the RD114 retrovirus.RD114-derived recombinant retroviruses were produced transiently by transfection of 293T cells, and viral titers were assessed on TE671 cells by measuring the percentage of infected green fluorescent protein (GFP) positive cells by fluorescence-activated cell sorter (FACS) analysis. Purified human hematopoietic cells (lymphocytes and CD34+ cells) were activated and transduced on retronectin-coated plates. Two days later, the percentage of GFP positive cells was evaluated by FACS analysis.We demonstrate that RD114 viral...
Source: The Journal of Gene Medicine - June 8, 2009 Category: Genetics & Stem Cells Authors: Karim Ghani, Sylvine Cottin, Pedro Otavio de Campos-Lima, Marie-Christine Caron, Manuel Caruso Source Type: journals

Stabilization of gammaretroviral and lentiviral vectors: from production to gene transferEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The low stability of gammaretroviral and lentiviral vectors affects their production, making high quality clinical preparations a difficult goal to achieve. Recently, our laboratory has shown that the main inactivation mechanism for both these vectors is the loss of their capacity to perform reverse transcription. The present study aimed to increase the stability of gammaretroviral and lentiviral at 37 °C and at 4 °C.Inactivation studies were performed with gammaretroviral and lentiviral vectors at 37 and 4 °C, with and without several stabilizing compounds. The residual viral infectivity and reverse transcription capac...
Source: The Journal of Gene Medicine - June 8, 2009 Category: Genetics & Stem Cells Authors: Marlene Carmo, Ana Alves, Ana Filipa Rodrigues, Ana Sofia Coroadinha, Manuel Jose Teixeira Carrondo, Paula Marques Alves, Pedro Estilita Cruz Source Type: journals

Treatment of disseminated ovarian cancer using nonviral interleukin-12 gene therapy delivered intraperitoneallyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The poor prognosis associated with ovarian cancer is primarily the result of delayed diagnosis and the lack of an effective treatment for advanced disease. Use of novel immunotherapy strategies are being evaluated that work to enhance local and systemic immune responses against cancer cells and can possibly work together with traditional cytotoxic chemotherapy regimens to produce more effective treatment options.In the present study, we describe a gene-based therapy whereby the anticancer cytokine interleukin-12 gene (pmIL-12) is formulated with a synthetic polymeric delivery vehicle (PPC) and administered intraperitoneall...
Source: The Journal of Gene Medicine - June 8, 2009 Category: Genetics & Stem Cells Authors: Jason G. Fewell, Majed M. Matar, Jennifer S. Rice, Elaine Brunhoeber, Gregory Slobodkin, Casey Pence, Miranda Worker, Danny H. Lewis, Khursheed Anwer Source Type: journals

TLR9-dependent systemic interferon-[beta] production by intravenous injection of plasmid DNA/cationic liposome complex in miceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The type I interferon (IFN) response to DNA/cationic liposome complex, or lipoplex, has been reported in cultured cells, but little is known about the response in vivo. Studies of the pro-inflammatory cytokine response to lipoplex have shown the importance of the unmethylated CpG dinucleotide (CpG motif) and its receptor, Toll-like receptor (TLR)-9.CpG- and non-CpG lipoplex consisting of CpG- or non-CpG plasmid DNA, respectively, and N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride/cholesterol liposomes were intravenously injected into mice. IFN-[beta] and interleukin (IL)-6 in the serum and organs were measur...
Source: The Journal of Gene Medicine - May 21, 2009 Category: Genetics & Stem Cells Authors: Hiroyuki Yoshida, Makiya Nishikawa, Sachiyo Yasuda, Yumiko Mizuno, Hiroyasu Toyota, Tsuyoshi Kiyota, Rei Takahashi, Yoshinobu Takakura Source Type: journals

Transient expression of genes delivered to newborn rat liver using recombinant adeno-associated virus 2/8 vectorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In vivo adeno-associated virus (AAV) delivery to adult liver results in sustained expression of the transgene. However, it has been suggested that AAV delivery to the newborn liver may result in transient expression. In the present study, we analysed transgene expression after AAV8 delivery of a therapeutic or a marker gene to newborn rat liver.Recombinant AAV 8 vectors carrying either the human UGT1A1 cDNA or the lacZ gene were injected intravenously in 2-day-old Gunn or Wistar rats. Serum bilirubin level was recorded in Gunn rats and [beta]-galactosidase expression was monitored by immunohistochemistry or enzyme activity...
Source: The Journal of Gene Medicine - May 19, 2009 Category: Genetics & Stem Cells Authors: Maude Flageul, Dominique Aubert, Virginie Pichard, Tuan Huy Nguyen, Ali Nowrouzi, Manfred Schmidt, Nicolas Ferry Source Type: journals

A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptideEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin.We inserted the ...
Source: The Journal of Gene Medicine - May 19, 2009 Category: Genetics & Stem Cells Authors: Kouki Morizono, Yiming Xie, Gustavo Helguera, Tracy R. Daniels, Timothy F. Lane, Manuel L. Penichet, Irvin S. Y. Chen Source Type: journals

Time dependency of ultrasound-facilitated gene transfectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The use of ultrasound (US)-facilitated gene therapy is increasing rapidly as a result of its high specificity and non-invasiveness. However, the acoustic parameters that produce the most efficient transfection have not been established. The present study investigated the effects of time parameters [including pulsing strategy (on- and off-times), exposure duration, pore opening time and expression duration] of US-facilitated gene transfection.Cervical cancer cells (HeLa cells) cultured with pCMViLUC plasmids were exposed to 1-MHz pulsed US, and gene transfection efficiency and cell viability were assessed. The ability of in...
Source: The Journal of Gene Medicine - May 19, 2009 Category: Genetics & Stem Cells Authors: Kun-Che Tsai, Shin-Yu Fang, Shu-Jyuan Yang, Ming-Jium Shieh, Win-Li Lin, Wen-Shiang Chen Source Type: journals