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[Clinical Utility of Fluorescence in-situ Hybridization Profile Test in Detecting Genetic Aberrations in Acute Leukemia.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: FISH analysis as a profile test detected additional genetic aberrations in a significant proportion of acute leukemia, and was effective especially in detecting cryptic translocations, submicroscopic deletions and complex karyotypes. Our study supports the need to incorporate FISH profile test at initial work up in acute leukemia. PMID: 19893343 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Kim SR, Kim HJ, Kim SH Tags: Korean J Lab Med Source Type: journals

[Detection of PML/RARA Rearrangement by Reverse Transcriptase-PCR and Sequencing in a Case of Microgranular Acute Promyelocytic Leukemia Lacking t(15;17) on Karyotype and FISH.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a case of morphologically microgranular acute promyelocytic leukemia with PML/RARA fusion transcripts demonstrated by reverse transcriptase-PCR and cDNA sequencing, and no PML/RARA fusion detected by karyotype and FISH analyses. Karyotype was 47,XX,+8[19]/46,XX[1]. Although the newer FISH probes provide more accurate detections of t(15;17), it would be necessary to perform other molecular tests to further identify the masked PML/RARA fusions. PMID: 19893344 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Kim KE, Woo KS, Kim SH, Han JY Tags: Korean J Lab Med Source Type: journals

[A case of light chain deposition disease involving kidney and bone marrow with microangiopathic hemolytic anemia.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a case of light chain deposition disease in a 59-yr-old female showing deposition of monoclonal light chain in the kidney and bone marrow accompanied with a schistocytosis, the morphologic finding of microangiopathic hemolytic anemia. The immunofluorescence examination of the kidney revealed strongly stained kappa-light chain deposits on the glomerular mesangium and capillary wall, tubules, and vessel wall. The electron microscopy demonstrated electron-dense deposits on the glomerular basement membrane and mesangium. Anemia was observed with schistocytosis and Howell-Jolly body in the peripheral blood smears. The...
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Cho YU, Chi HS, Park CJ, Jang S, Cho YM, Park JS Tags: Korean J Lab Med Source Type: journals

[Two Cases of Acute Myeloid Leukemia with t(16;21)(p11;q22) and TLS/FUS-ERG Fusion Transcripts.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report here 2 AML patients with t(16;21)(p11;q22), proved by conventional cytogenetics and/or reverse transcription (RT)-PCR. Erythrophagocytosis by leukemic blasts was observed in both of the cases. One patient was a 24 yr-old male with acute myelomonocytic leukemia. His karyotype was 46,XY,t(16;21)(p11;q22),del(18)(p11.2) and RT-PCR revealed the TLS/FUS-ERG fusion transcripts. Although he received allogeneic peripheral blood stem cell transplantation after the first remission, he died 9 months after the initial diagnosis due to relapse of the disease and graft-versus-host disease. The other patient was a 72 yr-old mal...
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Chang WR, Park IJ, Lee HW, Park JS, Kim HC, Kim HJ, Han JH, Cho SR Tags: Korean J Lab Med Source Type: journals

Discrepant Immunophenotypic Characteristics between the Lymph Node and Bone Marrow in Two Mixed-Phenotype Acute Leukemia Patients.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The immunophenotypic profile of hematological malignancies is usually consistent among different sites of involvement; this consistency allows reliable diagnosis from peripheral blood, bone marrow, or lymph node, especially in cases of acute leukemia. Although in a minority of lymphoma patients, two or more different populations with discordant immunophenotypes have been described, either at the same or distinct sites. Here, we report two Korean patients with acute leukemia where the results of immunophenotypic analysis of the bone marrow specimen were different from those of immunohistochemical studies of a biopsy sam...
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Song MJ, Kim HJ, Ko YH, Kim SH Tags: Korean J Lab Med Source Type: journals

Concurrent langerhans cell histiocytosis and B-lineage lymphoid proliferation in the bone marrow.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report three rare cases of concurrent bone marrow involvement of LCH and B-lineage lymphoid proliferation, which strongly suggest lymphoid malignancy. Further, clonal changes should be studied to elucidate the common pathogenic mechanism between the two diseases. PMID: 19893348 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Kim M, Kang HJ, Shin HY, Ahn HS, Lee DS Tags: Korean J Lab Med Source Type: journals

[Performance characteristics of glycated albumin and its clinical usefulness in diabetic patients on hemodialysis.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The claimed performance characteristic of Lucica GA-L were verified. WMG were better reflected by GA rather than HbA1c in patients on hemodialysis. PMID: 19893349 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Park HI, Kim YS, Lee J, Kim Y, Shin SJ Tags: Korean J Lab Med Source Type: journals

[Performance evaluation of affinity column mediated immunometric assay for tacrolimus.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The imprecision, linearity, detection limits, interference and correlation of Tac-CM with ACMIA were suitable for clinical use. Tac-CM with ACMIA could reduce turn around time and help clinicians to manage transplant patients on immunosuppressant therapy. PMID: 19893350 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Chung JW, An D, Song J, Chung HJ, Park HI, Lee W, Chun S, Min WK Tags: Korean J Lab Med Source Type: journals

[Analytical Performance Evaluation of Glucose Monitoring System Following ISO15197.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: This study showed that SureStep Flexx can provide reliable results for patients and clinicians to manage the diabetes mellitus, satisfying the ISO15197 criteria. PMID: 19893351 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: An D, Chung HJ, Lee HW, Lee W, Chun S, Min WK Tags: Korean J Lab Med Source Type: journals

[Performance Evaluation of the Piccolo xpress Point-of-care Chemistry Analyzer.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The Piccolo xpress Chemistry Analyzer can analyze multiple analytes with a minimal amount of whole blood in a short time. It showed an acceptable performance for precision, linearity and comparison with central laboratory analyzer. It can be useful as a screening tests modality in mobile clinics, ambulances, and field clinics for military use, and for pediatric patients from whom enough sample volume is difficult to obtain. PMID: 19893352 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Park H, Ko DH, Kim JQ, Song SH Tags: Korean J Lab Med Source Type: journals

[Performance Evaluation of BD GeneOhm MRSA PCR Assay for Detection of Nasal Colonization of Methicillin-Resistant Staphylococcus aureus at Endemic Intensive Care Units.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: GeneOhm MRSA PCR assay showed a relatively high negative predictive value. However, its low specificity and frequent occurrence of unresolved results would be problematic in the endemic areas with a high prevalence of MRSA. PMID: 19893353 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Park SH, Jang YH, Sung H, Kim MN, Kim JS, Park YJ Tags: Korean J Lab Med Source Type: journals

Comparison of 3 Phenotypic-detection Methods for Identifying Plasmid-mediated AmpC beta-lactamase-producing Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis Strains.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis. PMID: 19893354 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Lee W, Jung B, Hong SG, Song W, Jeong SH, Lee K, Kwak HS Tags: Korean J Lab Med Source Type: journals

Detection of Isoniazid and Rifampicin Resistance by Sequencing of katG, inhA, and rpoB Genes in Korea.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Detection of mutations in the katG codon 315, the inhA regulatory region, and the hot-spot region of rpoB would be useful for rapid detection of INH and RIF resistance in Korea. PMID: 19893355 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Cho EH, Bae HK, Kang SK, Lee EH Tags: Korean J Lab Med Source Type: journals

Case Report of Pediatric Gastroenteritis Due to CTX-M-15 Extended-Spectrum beta-Lactamase-Producing Salmonella enterica Serotype Enteritidis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A clinical isolate of Salmonella enterica serotype Enteritidis in Korea was found to produce the extended-spectrum beta-lactamase CTX-M-15. The isolate was recovered in 2008 from the stool of a 3-yr-old boy with gastroenteritis. This isolate was found to be resistant to multiple drugs, including ampicillin, piperacillin, cefotaxime, ceftazidime, cefepime, and aztreonam. The resistance to cefotaxime was transferred by conjugation to recipient Escherichia coli J53. The patient was eventually successfully treated with trimethoprim-sulfamethoxazole. This is the first report of the bla (CTX-M-15) gene in S. enterica serotyp...
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Lee KH, Song W, Jeong SH, Choi KY, Yoon HS, Park MJ Tags: Korean J Lab Med Source Type: journals

[Allergen Specific IgE Measurement with Polycheck Allergy: Comparison of Three Multiple Allergen Simultaneous Tests.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Since Polycheck Allergy showed similar or superior result to the others, it can be used for the detection of allergen specific IgE antibodies. PMID: 19893357 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Jang WR, Nahm CH, Kim JH, Lim DH, Jang TY, Moon YS, Kim JJ Tags: Korean J Lab Med Source Type: journals

[Comparative Evaluation of ELISA and Luminex Panel Reactive Antibody Assays for HLA Alloantibody Screening.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Different methods used to detect HLA antibodies showed discrepant results. As the Luminex method was more sensitive than ELISA for the detection of HLA antibodies, it can be used as a routine test in the transplantation laboratory. PMID: 19893358 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Jung S, Oh EJ, Yang CW, Ahn WS, Kim Y, Park YJ, Han K Tags: Korean J Lab Med Source Type: journals

[An Evaluation of Platelet Transfusion Response Using HLA Crossmatch-compatible Donors in Patients with Platelet Refractoriness.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Effective post-transfusion platelet increment using HLA crossmatch-compatible donors was attained in patients with platelet refractoriness due to HLA antibodies, and this method can be used effectively where HLA-typed platelet donor registry is not available. PMID: 19893359 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - October 1, 2009 Category: Laboratory Medicine Authors: Hyun J, Lim YM, Park KD, Han BY, Kim YH, Han KS, Park MH Tags: Korean J Lab Med Source Type: journals

[Detection of Putative T cell Clones Using T cell Receptor beta Chain Gene Clonality Assay in Korean Patients with Aplastic Anemia.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Putative pathogenetic T cell clones were detected in most of AA patients in the current study. T cell clonality assay would be useful for investigating the pathophysiology of acquired AA. PMID: 19726886 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Choi HJ, Shin MG, Kim HR, Kim HJ, Kook H, Kee SJ, Kim SH, Shin JH, Suh SP, Ryang DW Tags: Korean J Lab Med Source Type: journals

Clinical utility of chimerism status assessed by lineage-specific short tandem repeat analysis: experience from four cases of allogeneic stem cell transplantation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we serially monitored chimerism status by short tandem repeat-based PCR in nucleated cells (NC), T cells and natural killer (NK) cells after myeloablative allogeneic stem cell transplantation (SCT). Four patients with myeloid malignancies showed discrepant chimerism results among those three fractions. Three patients had mixed chimerism (MC) of donor/host T cells at a time point around the onset of chronic graft-versus-host disease (GVHD). In two patients with disease relapse, MC of NK cells preceded a morphological relapse or NK cells showed a higher percentage of patient cells compared to NC. Therefore, ou...
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Goh RY, Cho SS, Song YJ, Heo K, Oh SY, Kim SH, Kwon HC, Kim HJ, Han JY Tags: Korean J Lab Med Source Type: journals

A case of histiocytic sarcoma diagnosed by bone marrow biopsy in a patient suffering from Fever for 8 months.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a case of histiocytic sarcoma diagnosed by bone marrow biopsy. A 64-yr-old male was admitted for fever and weight loss that persisted for 8 months. The patient died undiagnosed on the 7th hospitalization day. A bone marrow biopsy performed just before the patient's death revealed diffuse proliferation of large pleomorphic neoplastic cells with large, round to oval nuclei, vesicular chromatin, and abundant foamy cytoplasm. These cells were positive for histiocytic markers, CD68, lysozyme, CD21, and S-100 protein, but negative for B-cell, T/NK-cell, and epithelial cell markers, thus confirming the presence of his...
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Jang YH, Park CJ, Huh JR, Jang S, Chi HS Tags: Korean J Lab Med Source Type: journals

[The Relationship between Thyroid Function and the Risk Factors of Cardiovascular Disease at Female Medical Checkups.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: For the interpretation of FT4, its reference interval needs to be divided into 4 quartiles, which can be used as one of the predicting factors of the metabolic syndrome. PMID: 19726889 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Nah EH, Lee JG Tags: Korean J Lab Med Source Type: journals

Resistance Trends of Bacteroides fragilis Group Over an 8-Year Period, 1997-2004, in Korea.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Piperacillin-tazobactam, cefoxitin, imipenem, chloramphenicol, and metronidazole are still active against B. fragilis group isolates, while clindamycin no longer has a value as an empirical therapeutic agent in Korea. Furthermore, this study identified the first imipenem-resistant B. fragilis group isolates in Korea. PMID: 19726890 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Roh KH, Kim S, Kim CK, Yum JH, Kim MS, Yong D, Lee K, Kim JM, Chong Y Tags: Korean J Lab Med Source Type: journals

[Microbiological and Epidemiological Characteristics of Vancomycin-dependent Enterococci.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: VDE were not rare and seemed to emerge independently from VRE with a prolonged use of vancomycin. Vancomycin-dependence was reverted within several passages. PMID: 19726891 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Hwang K, Sung H, Namgoong S, Yoon NS, Kim MN Tags: Korean J Lab Med Source Type: journals

[Evaluation of Seeplex(TM) Pneumobacter Multiplex PCR Kit for the Detection of Respiratory Bacterial Pathogens in Pediatric Patients.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Seeplex(TM) Pneumobacter detection kit could be a useful screening tool for the rapid detection of respiratory bacterial pathogens. Further studies with lower respiratory tract specimens would be needed for the clinical evaluation of S. pneumoniae and H. influenzae detected by multiplex PCR. PMID: 19726892 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Park J, Kim JK, Rheem I, Kim J Tags: Korean J Lab Med Source Type: journals

Strain Typing of Mycobacterium tuberculosis Isolates from Korea by Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: MIRU-VNTR analysis by using well-selected loci can be useful in discriminating the clinical M. tuberculosis isolates in areas where the Beijing family is predominant. PMID: 19726893 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Yun KW, Song EJ, Choi GE, Hwang IK, Lee EY, Chang CL Tags: Korean J Lab Med Source Type: journals

The Evaluation of SD Bioline Strep A Rapid Antigen Test in Acute Pharyngitis in Pediatric Clinics.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The SD Bioline Strep A RAT kit can be useful as an alternative to throat cultures in the clinics for rapid diagnosis of GAS pharyngitis and for an early decision on the use of antibiotics. PMID: 19726894 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Kim S Tags: Korean J Lab Med Source Type: journals

The optimal wavelength of spectrophotometric broth microdilution antifungal susceptibility test for flucytosine and three azoles.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The range between 600 nm and 700 nm was the optimal wavelength for broth microdilution antifungal susceptibility testing, although any wavelength within the visible light spectrum can be used. PMID: 19726895 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Lee JY, Lee EJ, Kim SH, Jeong HS, Oh SH, Kim HR, Lee JN, Chang CL, Kho WG, Shin JH Tags: Korean J Lab Med Source Type: journals

[Comparison of Quantitative Results among Two Automated Rapid Plasma Reagin (RPR) Assays and a Manual RPR Test.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The two automated RPR assay kits could not be used as an alternative to manual RPR test for quantitative analysis of RPR titer. As Mediace RPR shows a prozone effect at relatively low RPR titer, caution is needed in the interpretation of the measured values. PMID: 19726896 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Kim YS, Lee J, Lee HK, Kim H, Kwon HJ, Min KO, Seo EJ, Kim SY Tags: Korean J Lab Med Source Type: journals

[Anti-inflammatory effect of near-infrared irradiated cell culture media.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: NIR-irradiated medium supported vascular endothelial cell proliferation and showed an anti-inflammatory effect on fibroblast culture. These results can be used as basic data for future research on the clinical application of NIR. PMID: 19726897 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Kim SG, Shin IH, Choi CH, Choe JY Tags: Korean J Lab Med Source Type: journals

[Meta-Analysis for the Pooled Sensitivity and Specificity of anti-Human Immunodeficiency Virus Ab Rapid Tests.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: This meta-analysis for HIV ICA rapid tests showed good performance. In consideration of low positive predictive values of HIV rapid tests, confirmation by enzyme immunoassay or Western blot is still needed. This study would be helpful in evaluating and establishing proper performance guideline for those kits not fully evaluated. PMID: 19726898 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Yoo SJ, Sohn YH, Choi SE, Oh HB Tags: Korean J Lab Med Source Type: journals

[Analysis of characteristics of mononuclear cells remaining in the leukoreduction system chamber of trima accel(r) and their differentiation into dendritic cells.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The mononuclear cells in LRS chambers of Trima Accel(R) are an excellent alternative source of viable and functional human blood cells, which can be used for research purposes. PMID: 19726899 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Lee Y, Kim S, Lee ST, Kim HS, Baek EJ, Kim HJ, Lee M, Kim HO Tags: Korean J Lab Med Source Type: journals

Primary anti-D Immunization by DEL Red Blood Cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Extremely weak D variants called DEL are serologically detectable only by adsorption-elution techniques. A nucleotide change of exon 9 in RHD gene, RHD (K409K, 1227G>A) allelic variant is present in almost all the DEL individuals of East Asians. No DEL phenotype has yet been shown to induce a primary alloanti-D immunization in East Asia. A 68-yr-old D-negative Korean man was negative for anti-D at admission, and he developed alloanti-D after transfusion of red blood cells (RBC) from 4 apparently D-negative donors. Four donors who typed D-negative by routine serologic test were analyzed by real-time PCR for RHD gene ...
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Kim KH, Kim KE, Woo KS, Han JY, Kim JM, Park KU Tags: Korean J Lab Med Source Type: journals

Prenatal diagnosis of pallister-killian syndrome associated with pulmonary stenosis and right ventricular dilatation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pallister-Killian syndrome (PKS) is a rare disorder characterized cytogenetically by tetrasomy 12p for isochromosome of the short arm of chromosome 12. PKS is diagnosed by prenatal genetic analysis through chorionic villous sampling, genetic amniocentesis, and cordocentesis, or by chromosomal analysis of skin fibroblasts, but is not usually detected by chromosomal analysis of peripheral blood cells. Herein, we report a case of a gravida at 23 weeks gestation with pulmonary stenosis and right ventricular dilation of the heart which were detected by sonography. Fluorescence in situ hybridization and a multicolor banding ...
Source: The Korean Journal of Laboratory Medicine - July 31, 2009 Category: Laboratory Medicine Authors: Park IY, Shin JC, Kwon JY, Koo BK, Kim M, Lim J, Kim Y, Han K Tags: Korean J Lab Med Source Type: journals

[Complete blood count reference values of donated cord blood from korean neonates.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: We established cord blood CBC reference values of healthy Korean neonates using a large-scale CB units. The established CBC reference values from our study will be useful as basic data for CBC interpretation and assessment of transplant suitability of donated CB. PMID: 19571613 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Lee HR, Shin S, Yoon JH, Kim BJ, Hwang KR, Kim JJ, Roh EY Tags: Korean J Lab Med Source Type: journals

[Incidence and causes of hyperosinophilia in the patients of a university hospital.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: We found a difference in the distribution of causes of hypereosinophilia in comparison with previous Korean studies, and the most common cause of hypereosinophilia in the current study was malignancy. A rare case of clonal eosinophilia (chronic eosinophilic leukemia) associated with multiple myeloma was confirmed using molecular studies. PMID: 19571614 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Kim da W, Shin MG, Yun HK, Kim SH, Shin JH, Suh SP, Ryang DW Tags: Korean J Lab Med Source Type: journals

A case of natural killer cell leukemia misdiagnosed as tuberculous lymphadenopathy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report here one case of NK cell leukemia. A 54-yr-old woman presented with a 2-month history of progressive left neck mass. Based on the positive result of tissue PCR for Mycobacterium tuberculosis, she was at first diagnosed with tuberculous lymphadenopathy. After two weeks, she developed generalized lymphadenopathy, hepatosplenomegaly, fever and anemia. Subsequent evaluation was performed including bone marrow aspiration and biopsy. Peripheral blood smear showed leukoerythroblastic features with 31% blasts. Bone marrow was packed with agranular blastoid cells, which were periodic acid-Schiff (PAS) positive and myelope...
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Lee AJ, Kim SG, Jeon CH, Suh HS, Yoon GS, Seo AN Tags: Korean J Lab Med Source Type: journals

A Novel Translocation t(1;5)(p32;q31) that Was Not Associated with the TAL1 Rearrangement in a Case of T Lymphoblastic Leukemia/Lymphoma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chromosome 1 band p32 (1p32) aberrations are common in T lymphoblastic leukemia/lymphoma (T-ALL/LBL). Two types of 1p32 aberrations include translocations with different partners and submicroscopic interstitial deletion. Both aberrations are known to result in TAL1 gene deregulation. The t(1;5)(p32;q31) is a rare translocation of 1p32 in T-ALL. We now present the second case of t(1;5)(p32;q31) in T-ALL, which was present as a primary cytogenetic abnormality, with a review of the relevant literature. Interestingly, neither the translocation of the TAL1 gene nor aberrant expression of TAL1 protein was detected by fluores...
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Cho HS, Kim MK, Bae YK Tags: Korean J Lab Med Source Type: journals

Evaluation of Dipicolinic Acid-Based Mueller Hinton Agar Biplate for Detection of IMP-1 and VIM-2 type Metallo-beta-Lactamase in Imipenem Non-susceptible Gram Negative Bacilli.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Assays using DPA 300-MHA biplate were highly sensitive and specific for the detection of IMP-1 and VIM-2 type MBL-producing bacteria. In addition, it is easy to perform; so, it may be useful to apply this method for detection of IMP-1 and VIM-2 type MBL in clinical laboratories. PMID: 19571617 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Shin KS, Son BR, Koo SH, Lee SH, Ahn JB, Park SH, Hwang SY Tags: Korean J Lab Med Source Type: journals

[Evaluation of the BD Phoenix Automated Microbiology System SMIC/ID-2 Panel for Antimicrobial Susceptibility Testing of Streptococcus pneumoniae.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The Phoenix SMIC/ID-2 panel provided a simple and rapid susceptibility testing for S. pneumoniae, and the results were in a good agreement with those of Etest. The Phoenix system appears to be an effective automated system in clinical microbiology laboratories. PMID: 19571618 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Lee KK, Ryoo NH, Kim ST, Chae SL, Huh HJ Tags: Korean J Lab Med Source Type: journals

Prevalence of the Extended-Spectrum beta-Lactamase and qnr Genes in Clinical Isolates of Escherichia coli.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: CTX-M-type enzymes were the most common type of ESBL in E. coli isolates from Korea, and the qnr genes were not uncommon in ESBL-producing E. coli isolates. Dissemination of E. coli containing both ESBL and qnr genes could compromise the future usefulness of the expanded-spectrum antibiotics for the treatment of infections. PMID: 19571619 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Park Y, Kang HK, Bae IK, Kim J, Kim JS, Uh Y, Jeong SH, Lee K Tags: Korean J Lab Med Source Type: journals

[Analysis of Clinical Characteristics and S Gene Mutation of Hepatitis B Virus (HBV) in Patients with Hepatitis B Surface Antigen RIA Negative and HBV DNA Positive.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: HBV DNA-positive/HBsAg RIA-negative results were mostly observed in the patients treated with HBIG after liver transplantation, in whom HBIG escape mutations were found. Majority of these cases were positive in Architect HBsAg assay, and it is recommended to use other HBsAg immunoassay methods that are more sensitive than RIA in the detection limit as well as in the detection of escape mutant in hospitals performing liver transplantation. PMID: 19571620 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Sohn YH, Oh HB, Ko SY, Lim YS, Kwon OJ Tags: Korean J Lab Med Source Type: journals

Rapid ABO Genotyping Using Whole Blood without DNA Purification.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: This convenient multiplex ASPCR assay, performed using whole blood samples, provides a supplement to routine serological ABO typing and might also be useful in other genotyping applications. PMID: 19571621 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Lee SH, Park G, Yang YG, Lee SG, Kim SW Tags: Korean J Lab Med Source Type: journals

[A Hemolytic Transfusion Reaction due to Anti-Ku Antibody in a Patient with Knull Phenotype: The First Case in Korea.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This report is the first case of anti-Ku in a Knull phenotype person in Korea, who experienced a moderate hemolytic transfusion reaction. PMID: 19571622 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Kang MG, Lim YA, Lee KM Tags: Korean J Lab Med Source Type: journals

[Usefulness of Real-time Semi-quantitative PCR, JAK2 MutaScreen (TM) Kit for JAK2 V617F Screening.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: JAK2 MutaScreen(TM) kit that utilizes a real-time semi-quantitative PCR method is a useful tool for diagnosing MPDs precisely. It can be used to assess the grade of mutant allele burden as well as to screen JAK2 V617F simultaneously. PMID: 19571623 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Chae H, Lee JH, Lim J, Jung SW, Kim M, Kim Y, Han K, Cho BS, Cho SG, Lee JW, Min WS Tags: Korean J Lab Med Source Type: journals

[Comparative Quantification of Plasma hnRNP B1 mRNA in Non-small Cell Lung Cancer Patients by Real-time PCR.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Plasma hnRNP B1 mRNA was significantly increased in patients with lung cancer compared with that in patients with other benign lung diseases. Plasma hnRNP B1 mRNA may be useful as a potential marker for the detection of NSCLC. PMID: 19571624 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Kim JM, Hwang SH, Song EJ, Lee SY, Kim YD, Lee CH, Lee MK, Chang CL, Lee EY Tags: Korean J Lab Med Source Type: journals

[A Case of Lambda-expressing Pulmonary MALT Lymphoma with Dual Clonal Rearrangements of Kappa and Lambda Immunoglobulin Light Chain Gene.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report the first case in Korea of lambda-expressing MALT lymphoma that is shown to have dual clonal rearrangements of kappa and lambda immunoglobulin light chain gene by multiplex PCR. PMID: 19571625 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Oh HR, Lee MJ, Park G, Moon DS, Park YJ, Jang SJ Tags: Korean J Lab Med Source Type: journals

[Influence of the pre-analytical specimen storage conditions on the fecal occult blood test results.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: Buffer-tube minimizes false negative FOBT results during pre-analytical delay of specimen. The examinees using buffer-free containers need to be educated to hand in their specimens to laboratories as soon as possible. PMID: 19571626 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - May 31, 2009 Category: Laboratory Medicine Authors: Yoo SJ, Moon SJ, Gwack EH, Shin BM Tags: Korean J Lab Med Source Type: journals

Expansion and activation of natural killer cells for cancer immunotherapy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Natural killer (NK) cells can kill a wide range of cancer cells and are a promising tool for cell therapy of cancer. NK cells cytotoxicity is regulated by a balance between stimulatory and inhibitory signals. Interleukin-2 is known to increase NK cell cytotoxicity. Although many cytokines have been studied in efforts to induce durable NK cell expansions, most reports indicate a rather modest effect and the requirement for additional stimuli. We found that contact with the K562 myeloid leukemia cell line, genetically modified to express a membrane-bound form of interleukin-15 and the ligand for the costimulatory molecul...
Source: The Korean Journal of Laboratory Medicine - March 31, 2009 Category: Laboratory Medicine Authors: Cho D, Campana D Tags: Korean J Lab Med Source Type: journals

[A Case of a Korean Adult Affected by Type B Niemann-Pick Disease: Secondary Sea-blue Histiocytosis and Molecular Characterization.]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a case of a 32-yr-old female, who presented with thrombocytopenia without any neurologic involvement. Hepatosplenomegaly was detected by both physical examination and imaging studies, and a thoracic radiograph examination showed a pattern of interstitial lung disease. Biochemical tests revealed increased liver enzymes, cholesterol, triglyceride, and LDL-cholesterol, and decreased HDL-cholesterol. Sea-blue or foamy vacuolated histiocytes occurred in bone marrow and liver. Sequencing analysis of SMPD1 using genomic DNA from peripheral leukocytes identified a compound heterozygote of two mutations at exon 2: p.E246K...
Source: The Korean Journal of Laboratory Medicine - March 31, 2009 Category: Laboratory Medicine Authors: Cho YU, Chae JD, Lee WM, Woo JJ, Lee HB, Gong SJ, Park CJ, Kim GH, Yoo HW Tags: Korean J Lab Med Source Type: journals

The comparison of parathyroid hormone degradation effect by various protease inhibitors in blood specimen.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSIONS: The decrease in PTH levels in blood samples seemed to be due to the degradation of PTH by proteases. Various proteases, including especially serine proteases, would act together to degrade PTH in blood specimen. The PTH degradation may be inhibited in blood specimen with protease inhibitor cocktail. PMID: 19411775 [PubMed - in process] (Source: The Korean Journal of Laboratory Medicine)
Source: The Korean Journal of Laboratory Medicine - March 31, 2009 Category: Laboratory Medicine Authors: Kim YS, Kwon HJ, Lee HK Tags: Korean J Lab Med Source Type: journals